Belongs to the protein kinase superfamily. Ser Thr protein kinase family
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No orthologs found for this gene in the current database.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 576, 1745 |
| AccII | CGCG | 4 cut(s) | 191, 464, 2431, 2614 |
| AciI | CCGC | 5 cut(s) | 74, 189, 1031, 1718, 2429 |
| AclI | AACGTT | 1 cut(s) | 51 |
| AclWI | GGATC | 7 cut(s) | 131, 144, 187, 508, 965, 1046, 1082 |
| AcsI | RAATTY | 4 cut(s) | 593, 604, 1024, 1321 |
| AcuI | CTGAAG | 1 cut(s) | 383 |
| AcvI | CACGTG | 1 cut(s) | 620 |
| AfaI | GTAC | 9 cut(s) | 961, 1084, 1207, 1284, 1537, 1871, 2113, 2283, 2484 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 70, 202, 239, 1061 |
| AflIII | ACRYGT | 3 cut(s) | 619, 984, 2463 |
| AjnI | CCWGG | 2 cut(s) | 955, 2420 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 719, 751 |
| Alw21I | GWGCWC | 1 cut(s) | 1224 |
| Alw26I | GTCTC | 9 cut(s) | 87, 772, 815, 935, 1170, 1846, 2120, 2363, 2476 |
| AlwI | GGATC | 7 cut(s) | 131, 144, 187, 508, 965, 1046, 1082 |
| Ama87I | CYCGRG | 1 cut(s) | 1934 |
| AoxI | GGCC | 1 cut(s) | 1928 |
| ApeKI | GCWGC | 2 cut(s) | 718, 835 |
| ApoI | RAATTY | 4 cut(s) | 593, 604, 1024, 1321 |
| AseI | ATTAAT | 1 cut(s) | 1179 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 542, 731, 779 |
| AspS9I | GGNCC | 4 cut(s) | 126, 293, 740, 881 |
| AsuC2I | CCSGG | 4 cut(s) | 130, 508, 518, 1019 |
| AsuHPI | GGTGA | 5 cut(s) | 58, 178, 451, 1033, 1289 |
| AsuII | TTCGAA | 1 cut(s) | 1259 |
| AvaI | CYCGRG | 1 cut(s) | 1934 |
| AvaII | GGWCC | 4 cut(s) | 126, 293, 740, 881 |
| AxyI | CCTNAGG | 1 cut(s) | 369 |
| BamHI | GGATCC | 1 cut(s) | 136 |
| BanII | GRGCYC | 3 cut(s) | 65, 1128, 1224 |
| BbrPI | CACGTG | 1 cut(s) | 620 |
| Bbv12I | GWGCWC | 1 cut(s) | 1224 |
| BbvI | GCAGC | 2 cut(s) | 705, 822 |
| BccI | CCATC | 2 cut(s) | 1059, 1697 |
| BceAI | ACGGC | 2 cut(s) | 915, 1147 |
| BciT130I | CCWGG | 2 cut(s) | 957, 2422 |
| BcnI | CCSGG | 4 cut(s) | 130, 508, 518, 1019 |
| BcoDI | GTCTC | 9 cut(s) | 87, 772, 815, 935, 1170, 1846, 2120, 2363, 2476 |
| BfaI | CTAG | 8 cut(s) | 549, 1365, 1494, 1580, 1794, 1814, 1944, 1974 |
| BglII | AGATCT | 1 cut(s) | 2026 |
| BisI | GCNGC | 2 cut(s) | 719, 836 |
| BlpI | GCTNAGC | 1 cut(s) | 2397 |
| BlsI | GCNGC | 2 cut(s) | 720, 837 |
| Bme1390I | CCNGG | 6 cut(s) | 130, 508, 518, 957, 1019, 2422 |
| Bme18I | GGWCC | 4 cut(s) | 126, 293, 740, 881 |
| BmeT110I | CYCGRG | 1 cut(s) | 1934 |
| BmgT120I | GGNCC | 4 cut(s) | 126, 293, 740, 881 |
| BmiI | GGNNCC | 4 cut(s) | 138, 281, 903, 2427 |
| BmrFI | CCNGG | 6 cut(s) | 130, 508, 518, 957, 1019, 2422 |
| BmrI | ACTGGG | 1 cut(s) | 1326 |
| BmsI | GCATC | 8 cut(s) | 624, 1370, 1449, 1464, 1486, 1495, 1615, 2391 |
| BmuI | ACTGGG | 1 cut(s) | 1326 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 2208, 2240 |
| BpmI | CTGGAG | 2 cut(s) | 939, 2404 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 2397 |
| Bpu14I | TTCGAA | 1 cut(s) | 1259 |
| BpuMI | CCSGG | 4 cut(s) | 130, 508, 518, 1019 |
| BsaAI | YACGTR | 1 cut(s) | 620 |
| BsaBI | GATNNNNATC | 2 cut(s) | 197, 420 |
| BsaI | GGTCTC | 4 cut(s) | 815, 935, 2120, 2476 |
| BsaJI | CCNNGG | 2 cut(s) | 42, 2421 |
| BsaWI | WCCGGW | 2 cut(s) | 737, 2177 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 719, 749, 1126, 1156 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 70, 202, 239, 1061 |
| Bse1I | ACTGG | 5 cut(s) | 128, 1090, 1254, 1321, 1543 |
| Bse21I | CCTNAGG | 1 cut(s) | 369 |
| Bse3DI | GCAATG | 4 cut(s) | 36, 1351, 1852, 2358 |
| Bse8I | GATNNNNATC | 2 cut(s) | 197, 420 |
| BseBI | CCWGG | 2 cut(s) | 957, 2422 |
| BseDI | CCNNGG | 2 cut(s) | 42, 2421 |
| BseGI | GGATG | 6 cut(s) | 1070, 1258, 1477, 1630, 1972, 2220 |
| BseJI | GATNNNNATC | 2 cut(s) | 197, 420 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 70, 202, 239, 1061 |
| BseMI | GCAATG | 4 cut(s) | 36, 1351, 1852, 2358 |
| BseMII | CTCAG | 7 cut(s) | 80, 231, 360, 1202, 2262, 2388, 2389 |
| BseNI | ACTGG | 5 cut(s) | 128, 1090, 1254, 1321, 1543 |
| BseRI | GAGGAG | 3 cut(s) | 1680, 1992, 2538 |
| BseXI | GCAGC | 2 cut(s) | 705, 822 |
| BseYI | CCCAGC | 2 cut(s) | 311, 1373 |
| Bsh1236I | CGCG | 4 cut(s) | 191, 464, 2431, 2614 |
| BshFI | GGCC | 1 cut(s) | 1930 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1224 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1934 |
| BsiSI | CCGG | 7 cut(s) | 129, 507, 518, 738, 1019, 2095, 2178 |
| BslFI | GGGAC | 3 cut(s) | 218, 350, 1897 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 70, 202, 239, 1061 |
| BsmAI | GTCTC | 9 cut(s) | 87, 772, 815, 935, 1170, 1846, 2120, 2363, 2476 |
| BsmBI | CGTCTC | 1 cut(s) | 1170 |
| BsmFI | GGGAC | 3 cut(s) | 218, 350, 1897 |
| BsnI | GGCC | 1 cut(s) | 1930 |
| Bso31I | GGTCTC | 4 cut(s) | 815, 935, 2120, 2476 |
| BsoBI | CYCGRG | 1 cut(s) | 1934 |
| Bsp119I | TTCGAA | 1 cut(s) | 1259 |
| Bsp1286I | GDGCHC | 3 cut(s) | 65, 1128, 1224 |
| Bsp1407I | TGTACA | 1 cut(s) | 2482 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 2397 |
| Bsp68I | TCGCGA | 1 cut(s) | 2614 |
| BspACI | CCGC | 5 cut(s) | 74, 189, 1031, 1718, 2429 |
| BspANI | GGCC | 1 cut(s) | 1930 |
| BspCNI | CTCAG | 7 cut(s) | 81, 230, 361, 1201, 2263, 2388, 2389 |
| BspFNI | CGCG | 4 cut(s) | 191, 464, 2431, 2614 |
| BspHI | TCATGA | 2 cut(s) | 78, 273 |
| BspLI | GGNNCC | 4 cut(s) | 138, 281, 903, 2427 |
| BspPI | GGATC | 7 cut(s) | 131, 144, 187, 508, 965, 1046, 1082 |
| BspT104I | TTCGAA | 1 cut(s) | 1259 |
| BspTNI | GGTCTC | 4 cut(s) | 815, 935, 2120, 2476 |
| BsrDI | GCAATG | 4 cut(s) | 36, 1351, 1852, 2358 |
| BsrGI | TGTACA | 1 cut(s) | 2482 |
| BsrI | ACTGG | 5 cut(s) | 128, 1090, 1254, 1321, 1543 |
| BssECI | CCNNGG | 2 cut(s) | 42, 2421 |
| BssT1I | CCWWGG | 1 cut(s) | 42 |
| Bst2UI | CCWGG | 2 cut(s) | 957, 2422 |
| Bst4CI | ACNGT | 6 cut(s) | 284, 334, 570, 1102, 1334, 1950 |
| Bst6I | CTCTTC | 7 cut(s) | 402, 533, 826, 1173, 2176, 2361, 2556 |
| BstAUI | TGTACA | 1 cut(s) | 2482 |
| BstBAI | YACGTR | 1 cut(s) | 620 |
| BstBI | TTCGAA | 1 cut(s) | 1259 |
| BstC8I | GCNNGC | 5 cut(s) | 175, 1224, 2067, 2100, 2403 |
| BstEII | GGTNACC | 2 cut(s) | 184, 1013 |
| BstF5I | GGATG | 6 cut(s) | 1070, 1258, 1477, 1630, 1972, 2220 |
| BstFNI | CGCG | 4 cut(s) | 191, 464, 2431, 2614 |
| BstMAI | GTCTC | 9 cut(s) | 87, 772, 815, 935, 1170, 1846, 2120, 2363, 2476 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 174, 643, 1160, 1842, 2075, 2402 |
| BstNI | CCWGG | 2 cut(s) | 957, 2422 |
| BstNSI | RCATGY | 4 cut(s) | 407, 1876, 2065, 2069 |
| BstPI | GGTNACC | 2 cut(s) | 184, 1013 |
| BstSCI | CCNGG | 6 cut(s) | 128, 506, 516, 955, 1017, 2420 |
| BstUI | CGCG | 4 cut(s) | 191, 464, 2431, 2614 |
| BstV1I | GCAGC | 2 cut(s) | 705, 822 |
| BstX2I | RGATCY | 3 cut(s) | 136, 513, 2026 |
| BstYI | RGATCY | 3 cut(s) | 136, 513, 2026 |
| Bsu36I | CCTNAGG | 1 cut(s) | 369 |
| BsuRI | GGCC | 1 cut(s) | 1930 |
| BtsCI | GGATG | 6 cut(s) | 1070, 1258, 1477, 1630, 1972, 2220 |
| BtsI | GCAGTG | 2 cut(s) | 2178, 2515 |
| BtsIMutI | CAGTG | 4 cut(s) | 1247, 1567, 2178, 2515 |
| BtuMI | TCGCGA | 1 cut(s) | 2614 |
| Cac8I | GCNNGC | 5 cut(s) | 175, 1224, 2067, 2100, 2403 |
| CciI | TCATGA | 2 cut(s) | 78, 273 |
| Cfr13I | GGNCC | 4 cut(s) | 126, 293, 740, 881 |
| CseI | GACGC | 3 cut(s) | 179, 1152, 1580 |
| Csp6I | GTAC | 9 cut(s) | 960, 1083, 1206, 1283, 1536, 1870, 2112, 2282, 2483 |
| CviQI | GTAC | 9 cut(s) | 960, 1083, 1206, 1283, 1536, 1870, 2112, 2282, 2483 |
| DraI | TTTAAA | 1 cut(s) | 1771 |
| Eam1104I | CTCTTC | 7 cut(s) | 402, 533, 826, 1173, 2176, 2361, 2556 |
| EarI | CTCTTC | 7 cut(s) | 402, 533, 826, 1173, 2176, 2361, 2556 |
| Ecl136II | GAGCTC | 1 cut(s) | 1222 |
| Eco130I | CCWWGG | 1 cut(s) | 42 |
| Eco147I | AGGCCT | 1 cut(s) | 1930 |
| Eco24I | GRGCYC | 3 cut(s) | 65, 1128, 1224 |
| Eco31I | GGTCTC | 4 cut(s) | 815, 935, 2120, 2476 |
| Eco32I | GATATC | 2 cut(s) | 1096, 2524 |
| Eco47I | GGWCC | 4 cut(s) | 126, 293, 740, 881 |
| Eco53kI | GAGCTC | 1 cut(s) | 1222 |
| Eco57I | CTGAAG | 1 cut(s) | 383 |
| Eco72I | CACGTG | 1 cut(s) | 620 |
| Eco81I | CCTNAGG | 1 cut(s) | 369 |
| Eco88I | CYCGRG | 1 cut(s) | 1934 |
| Eco91I | GGTNACC | 2 cut(s) | 184, 1013 |
| EcoICRI | GAGCTC | 1 cut(s) | 1222 |
| EcoO65I | GGTNACC | 2 cut(s) | 184, 1013 |
| EcoRII | CCWGG | 2 cut(s) | 955, 2420 |
| EcoRV | GATATC | 2 cut(s) | 1096, 2524 |
| EcoT14I | CCWWGG | 1 cut(s) | 42 |
| EcoT22I | ATGCAT | 2 cut(s) | 1479, 2067 |
| EcoT38I | GRGCYC | 3 cut(s) | 65, 1128, 1224 |
| ErhI | CCWWGG | 1 cut(s) | 42 |
| Esp3I | CGTCTC | 1 cut(s) | 1170 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 2271, 2303 |
| FaqI | GGGAC | 3 cut(s) | 218, 350, 1897 |
| Fnu4HI | GCNGC | 2 cut(s) | 719, 836 |
| FokI | GGATG | 6 cut(s) | 1077, 1265, 1464, 1637, 1979, 2207 |
| FriOI | GRGCYC | 3 cut(s) | 65, 1128, 1224 |
| Fsp4HI | GCNGC | 2 cut(s) | 719, 836 |
| FspBI | CTAG | 8 cut(s) | 549, 1365, 1494, 1580, 1794, 1814, 1944, 1974 |
| GluI | GCNGC | 2 cut(s) | 719, 836 |
| GsaI | CCCAGC | 2 cut(s) | 315, 1377 |
| GsuI | CTGGAG | 2 cut(s) | 939, 2404 |
| HaeIII | GGCC | 1 cut(s) | 1930 |
| HapII | CCGG | 7 cut(s) | 129, 507, 518, 738, 1019, 2095, 2178 |
| HgaI | GACGC | 3 cut(s) | 179, 1152, 1580 |
| HincII | GTYRAC | 5 cut(s) | 493, 982, 1309, 2362, 2461 |
| HindII | GTYRAC | 5 cut(s) | 493, 982, 1309, 2362, 2461 |
| HindIII | AAGCTT | 1 cut(s) | 662 |
| HpaI | GTTAAC | 1 cut(s) | 1309 |
| HpaII | CCGG | 7 cut(s) | 129, 507, 518, 738, 1019, 2095, 2178 |
| HphI | GGTGA | 5 cut(s) | 58, 178, 451, 1033, 1289 |
| Hpy99I | CGWCG | 1 cut(s) | 173 |
| HpyAV | CCTTC | 8 cut(s) | 648, 1835, 1906, 1913, 2046, 2102, 2619, 2628 |
| HpyCH4III | ACNGT | 6 cut(s) | 284, 334, 570, 1102, 1334, 1950 |
| HpyCH4IV | ACGT | 5 cut(s) | 51, 619, 986, 2454, 2463 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 174, 643, 1160, 1842, 2075, 2402 |
| HpySE526I | ACGT | 5 cut(s) | 51, 619, 986, 2454, 2463 |
| KspAI | GTTAAC | 1 cut(s) | 1309 |
| LmnI | GCTCC | 3 cut(s) | 907, 2230, 2423 |
| Lsp1109I | GCAGC | 2 cut(s) | 705, 822 |
| LweI | GCATC | 8 cut(s) | 624, 1370, 1449, 1464, 1486, 1495, 1615, 2391 |
| MaeI | CTAG | 8 cut(s) | 549, 1365, 1494, 1580, 1794, 1814, 1944, 1974 |
| MaeII | ACGT | 5 cut(s) | 51, 619, 986, 2454, 2463 |
| MaeIII | GTNAC | 9 cut(s) | 184, 328, 712, 1013, 1235, 1496, 2173, 2185, 2510 |
| MfeI | CAATTG | 2 cut(s) | 1521, 2121 |
| MflI | RGATCY | 3 cut(s) | 136, 513, 2026 |
| MhlI | GDGCHC | 3 cut(s) | 65, 1128, 1224 |
| MlyI | GAGTC | 6 cut(s) | 89, 313, 553, 608, 2226, 2507 |
| MmeI | TCCRAC | 2 cut(s) | 114, 636 |
| Mph1103I | ATGCAT | 2 cut(s) | 1479, 2067 |
| MroXI | GAANNNNTTC | 3 cut(s) | 542, 731, 779 |
| MslI | CAYNNNNRTG | 1 cut(s) | 2225 |
| MspI | CCGG | 7 cut(s) | 129, 507, 518, 738, 1019, 2095, 2178 |
| MspR9I | CCNGG | 6 cut(s) | 130, 508, 518, 957, 1019, 2422 |
| MunI | CAATTG | 2 cut(s) | 1521, 2121 |
| MvaI | CCWGG | 2 cut(s) | 957, 2422 |
| MvnI | CGCG | 4 cut(s) | 191, 464, 2431, 2614 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 174, 643, 1160, 1842, 2075, 2402 |
| NciI | CCSGG | 4 cut(s) | 130, 508, 518, 1019 |
| NlaIV | GGNNCC | 4 cut(s) | 138, 281, 903, 2427 |
| NmuCI | GTSAC | 4 cut(s) | 184, 1496, 2173, 2510 |
| NruI | TCGCGA | 1 cut(s) | 2614 |
| NsiI | ATGCAT | 2 cut(s) | 1479, 2067 |
| NspI | RCATGY | 4 cut(s) | 407, 1876, 2065, 2069 |
| NspV | TTCGAA | 1 cut(s) | 1259 |
| PaeI | GCATGC | 1 cut(s) | 2069 |
| PaeR7I | CTCGAG | 1 cut(s) | 1934 |
| PagI | TCATGA | 2 cut(s) | 78, 273 |
| PceI | AGGCCT | 1 cut(s) | 1930 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 2460 |
| PdmI | GAANNNNTTC | 3 cut(s) | 542, 731, 779 |
| PfoI | TCCNGGA | 2 cut(s) | 506, 516 |
| PkrI | GCNGC | 2 cut(s) | 720, 837 |
| PleI | GAGTC | 6 cut(s) | 89, 312, 553, 608, 2225, 2507 |
| PmaCI | CACGTG | 1 cut(s) | 620 |
| PmlI | CACGTG | 1 cut(s) | 620 |
| PpsI | GAGTC | 6 cut(s) | 89, 312, 553, 608, 2225, 2507 |
| Ppu21I | YACGTR | 1 cut(s) | 620 |
| PshBI | ATTAAT | 1 cut(s) | 1179 |
| PsiI | TTATAA | 2 cut(s) | 576, 1745 |
| Psp124BI | GAGCTC | 1 cut(s) | 1224 |
| Psp1406I | AACGTT | 1 cut(s) | 51 |
| Psp6I | CCWGG | 2 cut(s) | 955, 2420 |
| PspCI | CACGTG | 1 cut(s) | 620 |
| PspEI | GGTNACC | 2 cut(s) | 184, 1013 |
| PspFI | CCCAGC | 2 cut(s) | 311, 1373 |
| PspGI | CCWGG | 2 cut(s) | 955, 2420 |
| PspN4I | GGNNCC | 4 cut(s) | 138, 281, 903, 2427 |
| PspPI | GGNCC | 4 cut(s) | 126, 293, 740, 881 |
| PsuI | RGATCY | 3 cut(s) | 136, 513, 2026 |
| RruI | TCGCGA | 1 cut(s) | 2614 |
| RsaI | GTAC | 9 cut(s) | 961, 1084, 1207, 1284, 1537, 1871, 2113, 2283, 2484 |
| RsaNI | GTAC | 9 cut(s) | 960, 1083, 1206, 1283, 1536, 1870, 2112, 2282, 2483 |
| RseI | CAYNNNNRTG | 1 cut(s) | 2225 |
| SacI | GAGCTC | 1 cut(s) | 1224 |
| SatI | GCNGC | 2 cut(s) | 719, 836 |
| Sau96I | GGNCC | 4 cut(s) | 126, 293, 740, 881 |
| SchI | GAGTC | 6 cut(s) | 89, 313, 553, 608, 2226, 2507 |
| ScrFI | CCNGG | 6 cut(s) | 130, 508, 518, 957, 1019, 2422 |
| SduI | GDGCHC | 3 cut(s) | 65, 1128, 1224 |
| SfaNI | GCATC | 8 cut(s) | 624, 1370, 1449, 1464, 1486, 1495, 1615, 2391 |
| Sfr274I | CTCGAG | 1 cut(s) | 1934 |
| SfuI | TTCGAA | 1 cut(s) | 1259 |
| SinI | GGWCC | 4 cut(s) | 126, 293, 740, 881 |
| SlaI | CTCGAG | 1 cut(s) | 1934 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 2225 |
| SmlI | CTYRAG | 1 cut(s) | 1934 |
| SmoI | CTYRAG | 1 cut(s) | 1934 |
| SphI | GCATGC | 1 cut(s) | 2069 |
| SseBI | AGGCCT | 1 cut(s) | 1930 |
| SsiI | CCGC | 5 cut(s) | 74, 189, 1031, 1718, 2429 |
| SspI | AATATT | 2 cut(s) | 1597, 2044 |
| SspMI | CTAG | 8 cut(s) | 549, 1365, 1494, 1580, 1794, 1814, 1944, 1974 |
| SstI | GAGCTC | 1 cut(s) | 1224 |
| StuI | AGGCCT | 1 cut(s) | 1930 |
| StyD4I | CCNGG | 6 cut(s) | 128, 506, 516, 955, 1017, 2420 |
| StyI | CCWWGG | 1 cut(s) | 42 |
| TaaI | ACNGT | 6 cut(s) | 284, 334, 570, 1102, 1334, 1950 |
| TaiI | ACGT | 5 cut(s) | 54, 622, 989, 2457, 2466 |
| TaqI | TCGA | 8 cut(s) | 59, 267, 531, 1259, 1641, 1739, 1935, 2245 |
| TatI | WGTACW | 5 cut(s) | 1082, 1535, 1869, 2281, 2482 |
| TscAI | CASTG | 4 cut(s) | 1254, 1567, 2178, 2515 |
| TseFI | GTSAC | 4 cut(s) | 184, 1496, 2173, 2510 |
| TseI | GCWGC | 2 cut(s) | 718, 835 |
| Tsp45I | GTSAC | 4 cut(s) | 184, 1496, 2173, 2510 |
| TspDTI | ATGAA | 7 cut(s) | 25, 148, 617, 656, 1814, 2005, 2462 |
| TspRI | CASTG | 4 cut(s) | 1254, 1567, 2178, 2515 |
| VpaK11BI | GGWCC | 4 cut(s) | 126, 293, 740, 881 |
| VspI | ATTAAT | 1 cut(s) | 1179 |
| XapI | RAATTY | 4 cut(s) | 593, 604, 1024, 1321 |
| XceI | RCATGY | 4 cut(s) | 407, 1876, 2065, 2069 |
| XhoI | CTCGAG | 1 cut(s) | 1934 |
| XmnI | GAANNNNTTC | 3 cut(s) | 542, 731, 779 |
| XspI | CTAG | 8 cut(s) | 549, 1365, 1494, 1580, 1794, 1814, 1944, 1974 |
| Zsp2I | ATGCAT | 2 cut(s) | 1479, 2067 |