AT1G66750
ERF Family

belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
N/A
Physical Location & Seq
Forward (+)
24894523 .. 24897259
2737 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G66750.1

Sequence Viewer

Length: 1047 bp
ATGTCGAAATCCGGCGATAATCAACCAGTTGATAGATACCTCCGCCGTCAAATCCTCGGAGAAGGAACATACGGTGTCGTTTACAAAGCCACCGACACAAAGACTGGGAAAACTGTAGCAGTGAAGAAGATAAGATTAGGAAACCAAAAGGAAGGAGTAAATTTCACAGCTTTAAGAGAAATCAAGCTACTAAAAGAGCTGAATCATCCACATATCGTTGAACTCATTGATGCTTTCCCTCACGATGGAAGTTTGCATCTAGTTTTTGAGTATATGCAAACAGATTTAGAAGCAGTGATTCGTGATCGTAATATCTTTTTATCTCCTGGTGATATTAAGTCTTATATGTTGATGACTTTGAAGGGTTTAGCTTATTGTCATAAGAAATGGGTTCTTCACAGGGATATGAAGCCGAATAATTTGTTGATTGGAGAGAATGGTTTGTTGAAGTTGGCTGATTTTGGTTTGGCGAGGTTGTTTGGGAGTCCGAATCGGAGGTTTACTCATCAGGTATTTGCTACATGGTACAGAGCGCCTGAGTTACTGTTTGGGAGTCGACAGTATGGAGCAGGAGTTGATGTTTGGGCTGCAGGCTGTATTTTTGCTGAGTTACTACTTCGTAGACCATTTCTTCCGGGTTCTACTGAGATTGATCAACTTGGAAAGATCTTTCAAGCGTTTGGTACTCCAGTACCTTCCCAATGGTCTGACATGATCTATCTCCCAGACTACATGGAATTCTCCTATACACCCGCTCCACCATTACGTACCATTTTCCCTATGGCAAGTGATGACGCTTTGGATCTTCTAGCAAAAATGTTCATCTACGACCCACGTCAAAGGATCACAATACAACAAGCTTTAGACCACAGGTATTTCTCGTCTTCTCCATCACCAACTGAGCCAGGGAAGCTTCAGATTCCAGCTTCCAAAGGAGACGCACTCGAACCAAAGGCCTCCGAGCAAAACCAACATGGAAATAGCCCAGCGGTACTGTCTCCTCCTGGTAAAATGAGGAGAGTGATGGGTCCTGAAGGATTCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000079 GO:0000428 GO:0001932 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004693 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005675 GO:0005737 GO:0006355 GO:0006357 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016301 GO:0016310 GO:0016591 GO:0016740 GO:0016772 GO:0016773 GO:0019219 GO:0019220 GO:0019222 GO:0019538 GO:0030880 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031974 GO:0031981 GO:0032268 GO:0032806 GO:0032991 GO:0036211 GO:0042325 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043549 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044798 GO:0045859 GO:0045893 GO:0045935 GO:0045944 GO:0048518 GO:0048522 GO:0048578 GO:0048580 GO:0048582 GO:0048583 GO:0048584 GO:0048586 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0051094 GO:0051171 GO:0051173 GO:0051174 GO:0051239 GO:0051240 GO:0051246 GO:0051252 GO:0051254 GO:0051338 GO:0051726 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0065009 GO:0070013 GO:0070985 GO:0071704 GO:0071900 GO:0080090 GO:0090575 GO:0097472 GO:0140096 GO:1901564 GO:1902494 GO:1902554 GO:1902680 GO:1902911 GO:1903506 GO:1903508 GO:1904029 GO:1990234 GO:2000026 GO:2000028 GO:2000112 GO:2000241 GO:2000243 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03022 ko03420 ko04110 map03022 map03420 map04110
Pfam Domains
Protein Families
Pkinase

Protein Analysis

348

Amino Acids

39.2

Weight (kDa)

9.11

Isoelectric Point (pI)

45.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 15 - 293 3.9e-73 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 17 - 239 5.5e-32 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 755
AccI GTMKAC 2 cut(s) 556, 622
AciI CCGC 3 cut(s) 43, 753, 989
AclWI GGATC 2 cut(s) 810, 851
AcsI RAATTY 2 cut(s) 160, 737
AcuI CTGAAG 1 cut(s) 899
AfaI GTAC 5 cut(s) 527, 685, 693, 769, 993
AfiI CCNNNNNNNGG 1 cut(s) 245
AgsI TTSAA 4 cut(s) 221, 361, 448, 674
AjiI CACGTC 1 cut(s) 836
AjnI CCWGG 3 cut(s) 325, 904, 1003
AluBI AGCT 7 cut(s) 170, 187, 199, 371, 860, 913, 926
AluI AGCT 7 cut(s) 170, 187, 199, 371, 860, 913, 926
Alw26I GTCTC 2 cut(s) 930, 1002
AlwI GGATC 2 cut(s) 810, 851
AoxI GGCC 1 cut(s) 954
ApeKI GCWGC 1 cut(s) 587
ApoI RAATTY 2 cut(s) 160, 737
AspLEI GCGC 1 cut(s) 535
AspS9I GGNCC 1 cut(s) 1028
AsuC2I CCSGG 1 cut(s) 636
AsuHPI GGTGA 2 cut(s) 341, 885
AvaII GGWCC 1 cut(s) 1028
BaeI ACNNNNGTAYC 4 cut(s) 675, 675, 708, 708
BbsI GAAGAC 1 cut(s) 876
BbvI GCAGC 1 cut(s) 574
BccI CCATC 3 cut(s) 239, 898, 1018
BceAI ACGGC 1 cut(s) 30
BciT130I CCWGG 3 cut(s) 327, 906, 1005
BclI TGATCA 1 cut(s) 652
BcnI CCSGG 1 cut(s) 636
BcoDI GTCTC 2 cut(s) 930, 1002
BfaI CTAG 2 cut(s) 260, 809
BfmI CTRYAG 2 cut(s) 114, 588
BfoI RGCGCY 1 cut(s) 536
BglII AGATCT 1 cut(s) 666
BisI GCNGC 1 cut(s) 588
BlsI GCNGC 1 cut(s) 589
Bme1390I CCNGG 4 cut(s) 327, 636, 906, 1005
Bme18I GGWCC 1 cut(s) 1028
BmgBI CACGTC 1 cut(s) 836
BmgT120I GGNCC 1 cut(s) 1028
BmiI GGNNCC 1 cut(s) 1029
BmrFI CCNGG 4 cut(s) 327, 636, 906, 1005
BmrI ACTGGG 1 cut(s) 114
BmsI GCATC 2 cut(s) 220, 265
BmuI ACTGGG 1 cut(s) 114
BoxI GACNNNNGTC 1 cut(s) 834
BpiI GAAGAC 1 cut(s) 876
BplI GAGNNNNNCTC 4 cut(s) 487, 519, 927, 959
BpmI CTGGAG 1 cut(s) 672
BpuMI CCSGG 1 cut(s) 636
BsaAI YACGTR 1 cut(s) 767
BsaJI CCNNGG 2 cut(s) 55, 905
BsaXI ACNNNNNCTCC 6 cut(s) 558, 564, 588, 594, 739, 769
Bsc4I CCNNNNNNNGG 1 cut(s) 245
Bse1I ACTGG 3 cut(s) 26, 109, 689
BseBI CCWGG 3 cut(s) 327, 906, 1005
BseDI CCNNGG 2 cut(s) 55, 905
BseGI GGATG 1 cut(s) 205
BseLI CCNNNNNNNGG 1 cut(s) 245
BseMII CTCAG 4 cut(s) 528, 597, 636, 891
BseNI ACTGG 3 cut(s) 26, 109, 689
BseRI GAGGAG 2 cut(s) 990, 1030
BseXI GCAGC 1 cut(s) 574
BseYI CCCAGC 1 cut(s) 985
BshFI GGCC 1 cut(s) 956
BsiSI CCGG 2 cut(s) 12, 635
BslI CCNNNNNNNGG 1 cut(s) 245
BsmAI GTCTC 2 cut(s) 930, 1002
BsmBI CGTCTC 1 cut(s) 930
BsnI GGCC 1 cut(s) 956
Bsp143I GATC 6 cut(s) 304, 652, 666, 714, 802, 843
BspACI CCGC 3 cut(s) 43, 753, 989
BspANI GGCC 1 cut(s) 956
BspCNI CTCAG 4 cut(s) 529, 598, 637, 892
BspLI GGNNCC 1 cut(s) 1029
BspMAI CTGCAG 1 cut(s) 592
BspPI GGATC 2 cut(s) 810, 851
BsrBI CCGCTC 1 cut(s) 755
BsrI ACTGG 3 cut(s) 26, 109, 689
BssECI CCNNGG 2 cut(s) 55, 905
BssMI GATC 6 cut(s) 304, 652, 666, 714, 802, 843
Bst2UI CCWGG 3 cut(s) 327, 906, 1005
Bst4CI ACNGT 5 cut(s) 74, 115, 546, 561, 996
BstBAI YACGTR 1 cut(s) 767
BstC8I GCNNGC 1 cut(s) 592
BstDEI CTNAG 4 cut(s) 537, 606, 645, 900
BstF5I GGATG 1 cut(s) 205
BstH2I RGCGCY 1 cut(s) 536
BstHHI GCGC 1 cut(s) 535
BstKTI GATC 6 cut(s) 307, 655, 669, 717, 805, 846
BstMAI GTCTC 2 cut(s) 930, 1002
BstMBI GATC 6 cut(s) 304, 652, 666, 714, 802, 843
BstMWI GCNNNNNNNGC 1 cut(s) 910
BstNI CCWGG 3 cut(s) 327, 906, 1005
BstPAI GACNNNNGTC 1 cut(s) 834
BstSCI CCNGG 4 cut(s) 325, 634, 904, 1003
BstSFI CTRYAG 2 cut(s) 114, 588
BstSNI TACGTA 1 cut(s) 767
BstV1I GCAGC 1 cut(s) 574
BstV2I GAAGAC 1 cut(s) 876
BstX2I RGATCY 2 cut(s) 666, 802
BstYI RGATCY 2 cut(s) 666, 802
BsuRI GGCC 1 cut(s) 956
BtrI CACGTC 1 cut(s) 836
BtsCI GGATG 1 cut(s) 205
BtsI GCAGTG 2 cut(s) 126, 300
BtsIMutI CAGTG 2 cut(s) 126, 300
Cac8I GCNNGC 1 cut(s) 592
CfoI GCGC 1 cut(s) 535
Cfr13I GGNCC 1 cut(s) 1028
CseI GACGC 2 cut(s) 803, 947
Csp6I GTAC 5 cut(s) 526, 684, 692, 768, 992
CviAII CATG 4 cut(s) 522, 712, 733, 974
CviQI GTAC 5 cut(s) 526, 684, 692, 768, 992
DdeI CTNAG 4 cut(s) 537, 606, 645, 900
DpnI GATC 6 cut(s) 306, 654, 668, 716, 804, 845
DpnII GATC 6 cut(s) 304, 652, 666, 714, 802, 843
EciI GGCGGA 1 cut(s) 32
Eco105I TACGTA 1 cut(s) 767
Eco147I AGGCCT 1 cut(s) 956
Eco47I GGWCC 1 cut(s) 1028
Eco57I CTGAAG 1 cut(s) 899
EcoO109I RGGNCCY 1 cut(s) 1028
EcoRI GAATTC 1 cut(s) 737
EcoRII CCWGG 3 cut(s) 325, 904, 1003
Esp3I CGTCTC 1 cut(s) 930
FaeI CATG 4 cut(s) 525, 715, 736, 977
FatI CATG 4 cut(s) 521, 711, 732, 973
FauI CCCGC 1 cut(s) 760
FbaI TGATCA 1 cut(s) 652
FblI GTMKAC 2 cut(s) 556, 622
Fnu4HI GCNGC 1 cut(s) 588
FokI GGATG 1 cut(s) 192
Fsp4HI GCNGC 1 cut(s) 588
FspBI CTAG 2 cut(s) 260, 809
GlaI GCGC 1 cut(s) 534
GluI GCNGC 1 cut(s) 588
GsaI CCCAGC 1 cut(s) 989
GsuI CTGGAG 1 cut(s) 672
HaeII RGCGCY 1 cut(s) 536
HaeIII GGCC 1 cut(s) 956
HapII CCGG 2 cut(s) 12, 635
HgaI GACGC 2 cut(s) 803, 947
HhaI GCGC 1 cut(s) 535
Hin1II CATG 4 cut(s) 525, 715, 736, 977
Hin6I GCGC 1 cut(s) 533
HinP1I GCGC 1 cut(s) 533
HincII GTYRAC 1 cut(s) 557
HindII GTYRAC 1 cut(s) 557
HindIII AAGCTT 2 cut(s) 858, 911
HinfI GANTC 7 cut(s) 202, 298, 484, 490, 553, 919, 1038
HpaII CCGG 2 cut(s) 12, 635
HphI GGTGA 2 cut(s) 341, 885
Hpy166II GTNNAC 4 cut(s) 82, 501, 557, 623
Hpy188I TCNGA 6 cut(s) 59, 489, 495, 709, 918, 961
Hpy188III TCNNGA 3 cut(s) 242, 302, 1031
Hpy8I GTNNAC 4 cut(s) 82, 501, 557, 623
HpyAV CCTTC 5 cut(s) 56, 146, 355, 705, 1028
HpyCH4III ACNGT 5 cut(s) 74, 115, 546, 561, 996
HpyCH4IV ACGT 2 cut(s) 766, 835
HpyCH4V TGCA 3 cut(s) 256, 277, 590
HpyF10VI GCNNNNNNNGC 1 cut(s) 910
HpyF3I CTNAG 4 cut(s) 537, 606, 645, 900
HpySE526I ACGT 2 cut(s) 766, 835
Hsp92II CATG 4 cut(s) 525, 715, 736, 977
HspAI GCGC 1 cut(s) 533
Ksp22I TGATCA 1 cut(s) 652
Kzo9I GATC 6 cut(s) 304, 652, 666, 714, 802, 843
LmnI GCTCC 2 cut(s) 566, 760
Lsp1109I GCAGC 1 cut(s) 574
LweI GCATC 2 cut(s) 220, 265
MaeI CTAG 2 cut(s) 260, 809
MaeII ACGT 2 cut(s) 766, 835
MaeIII GTNAC 2 cut(s) 540, 609
MalI GATC 6 cut(s) 306, 654, 668, 716, 804, 845
MbiI CCGCTC 1 cut(s) 755
MboI GATC 6 cut(s) 304, 652, 666, 714, 802, 843
MboII GAAGA 6 cut(s) 136, 139, 386, 623, 797, 876
MflI RGATCY 2 cut(s) 666, 802
MluCI AATT 3 cut(s) 160, 418, 737
MlyI GAGTC 2 cut(s) 493, 562
MnlI CCTC 8 cut(s) 50, 65, 249, 465, 489, 967, 1008, 1011
MseI TTAA 2 cut(s) 173, 336
MspA1I CMGCKG 1 cut(s) 989
MspI CCGG 2 cut(s) 12, 635
MspR9I CCNGG 4 cut(s) 327, 636, 906, 1005
MvaI CCWGG 3 cut(s) 327, 906, 1005
MwoI GCNNNNNNNGC 1 cut(s) 910
NciI CCSGG 1 cut(s) 636
NdeII GATC 6 cut(s) 304, 652, 666, 714, 802, 843
NlaIII CATG 4 cut(s) 525, 715, 736, 977
NlaIV GGNNCC 1 cut(s) 1029
PceI AGGCCT 1 cut(s) 956
PfeI GAWTC 5 cut(s) 202, 298, 490, 919, 1038
PkrI GCNGC 1 cut(s) 589
PleI GAGTC 2 cut(s) 492, 561
PpsI GAGTC 2 cut(s) 492, 561
Ppu21I YACGTR 1 cut(s) 767
PpuMI RGGWCCY 1 cut(s) 1028
PshAI GACNNNNGTC 1 cut(s) 834
Psp5II RGGWCCY 1 cut(s) 1028
Psp6I CCWGG 3 cut(s) 325, 904, 1003
PspFI CCCAGC 1 cut(s) 985
PspGI CCWGG 3 cut(s) 325, 904, 1003
PspN4I GGNNCC 1 cut(s) 1029
PspPI GGNCC 1 cut(s) 1028
PspPPI RGGWCCY 1 cut(s) 1028
PstI CTGCAG 1 cut(s) 592
PsuI RGATCY 2 cut(s) 666, 802
RsaI GTAC 5 cut(s) 527, 685, 693, 769, 993
RsaNI GTAC 5 cut(s) 526, 684, 692, 768, 992
SalI GTCGAC 1 cut(s) 555
SaqAI TTAA 2 cut(s) 173, 336
SatI GCNGC 1 cut(s) 588
Sau3AI GATC 6 cut(s) 304, 652, 666, 714, 802, 843
Sau96I GGNCC 1 cut(s) 1028
SchI GAGTC 2 cut(s) 493, 562
ScrFI CCNGG 4 cut(s) 327, 636, 906, 1005
SfaNI GCATC 2 cut(s) 220, 265
SfcI CTRYAG 2 cut(s) 114, 588
SinI GGWCC 1 cut(s) 1028
SnaBI TACGTA 1 cut(s) 767
Sse9I AATT 3 cut(s) 160, 418, 737
SseBI AGGCCT 1 cut(s) 956
SsiI CCGC 3 cut(s) 43, 753, 989
SspMI CTAG 2 cut(s) 260, 809
StuI AGGCCT 1 cut(s) 956
StyD4I CCNGG 4 cut(s) 325, 634, 904, 1003
TaaI ACNGT 5 cut(s) 74, 115, 546, 561, 996
TaiI ACGT 2 cut(s) 769, 838
TaqI TCGA 3 cut(s) 5, 556, 945
TasI AATT 3 cut(s) 160, 418, 737
TfiI GAWTC 5 cut(s) 202, 298, 490, 919, 1038
Tru1I TTAA 2 cut(s) 173, 336
Tru9I TTAA 2 cut(s) 173, 336
TscAI CASTG 2 cut(s) 126, 300
TseI GCWGC 1 cut(s) 587
TspDTI ATGAA 2 cut(s) 422, 811
TspRI CASTG 2 cut(s) 126, 300
VpaK11BI GGWCC 1 cut(s) 1028
XapI RAATTY 2 cut(s) 160, 737
XcmI CCANNNNNNNNNTGG 1 cut(s) 778
XmiI GTMKAC 2 cut(s) 556, 622
XspI CTAG 2 cut(s) 260, 809
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.