AT1G70110

Legume lectin domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
N/A
Physical Location & Seq
Reverse (-)
26406238 .. 26408343
2106 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G70110.1

Sequence Viewer

Length: 2001 bp
ATGGTGTTGTTGTTGTTTCTGGTACTGTTCTTCGTCCCCGAGAGCGTCGTATGTCAGAGACCAAACCCAAACGGTGTAGAGTTCAACACCAGTGGAAATATGTACACCAGTGGATCAGCATATATAAACAACAATGGTCTGATCAGACTCACAAATTCTACACCACAAACAACTGGTCAAGTTTTCTATAATGACCAACTTCGATTCAAGAACTCAGTGAACGGTACAGTTTCTTCCTTCTCCACCACTTTCGTTTTCTCTATTGAGTTTCATAATGGAATATATGGAGGATATGGAATTGCCTTTGTTATCTGTCCAACGAGAGATCTGTCTCCTACTTTTCCCACCACTTACCTTGGACTTTTCAATAGGAGCAACATGGGAGATCCAAAAAATCATATAGTAGCTGTTGAGCTCGATACTAAAGTTGACCAACAATTCGAGGACAAAGATGCTAACCATGTTGGCATTGATATCAATACTTTAGTTTCAGATACAGTTGCTTTAGCGGGTTATTACATGGATAACGGTACATTTCGAAGTCTCTTGCTTAACAGTGGACAACCTATGCAAATCTGGATTGAGTATGACAGTAAACAGAAACAGATTAATGTCACACTTCATCCTCTTTATGTCCCTAAGCCAAAGATTCCACTTCTTTCTCTTGAAAAGGATCTTTCTCCATATTTGCTTGAACTTATGTATGTTGGATTCACTTCAACTACAGGGGACCTAACTGCATCTCACTATATATTGGGATGGACGTTCAAGATGAATGGGACTACTCCAGATATAGACCCCTCTCGCCTTCCCAAAATTCCTCGTTATAATCAGCCATGGATCCAGAGTCCAAATGGGATTTTGACAATCAGTTTAACTGTATCAGGAGTCATCATACTCATTATCTTGAGTCTCAGTTTATGGCTTTTCCTGAAGAGGAAGAAGCTATTGGAAGTTCTTGAGGACTGGGAAGTTCAGTTTGGTCCTCATAGGTTTGCTTTCAAAGATCTGCATATTGCTACAAAGGGTTTCAAGGATACTGAGGTTCTTGGTAAAGGAGGATTTGGGAAAGTCTATAAAGGAACTTTACCAGTGTCCAACGTAGAGATAGCGGTGAAGATGGTTTCTCATGACTCAAGACAAGGAATGAGAGAGTTTATTGCTGAGATTGCAACCATTGGGCGTCTCAGACATCCTAACTTAGTTCGGCTTCAAGGCTACTGCAGACACAAAGGTGAACTCTACTTGGTGTATGATTGTATGGCCAAAGGAAGTCTTGATAAGTTCCTTTACCATCAACAGACTGGGAATCTTGATTGGTCACAGAGATTCAAGATCATCAAAGACGTAGCTTCTGGACTTTATTATTTGCACCAACAATGGGTGCAGGTCATTATCCACAGGGATATTAAACCAGCCAACATCCTTCTTGACGCTAATATGAATGCTAAACTTGGTGACTTTGGTCTCGCGAAGCTCTGCGATCATGGGACTGATCCTCAGACCTCTCATGTGGCAGGTACACTTGGGTATATTTCACCTGAGCTCTCAAGAACAGGAAAGGCAAGTACAAGATCAGATGTATTTGCGTTTGGAATAGTGATGCTAGAGATTGCTTGCGGGCGAAAACCCATATTGCCACGAGCATCACAAAGAGAGATGGTACTGACTGATTGGGTGCTAGAGTGTTGGGAGAATGAAGATATCATGCAGGTTCTTGATCATAAGATTGGTCAGGAATATGTTGAGGAGCAAGCGGCACTTGTTCTGAAACTTGGCTTATTCTGTTCTCATCCAGTAGCAGCCATCAGACCAAACATGTCTAGCGTCATTCAATTATTGGACAGTGTGGCTCAGCTTCCTCATAACTTACTTGACATTGTGCAAACTCGAGAAGTTCATCGAGGTACTGAAATCTCAGGTGAAGCAGCTGATTCTCCAGAGTCCTGTTCCATCGCTCCCTTGACCTTCACTGAATCCTTTGTCTCTCATGGTCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lectin_legB Pkinase

Protein Analysis

666

Amino Acids

74.58

Weight (kDa)

6.47

Isoelectric Point (pI)

36.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lectin_legB PF00139 28 - 271 3.6e-71 Legume lectin domain
Lectin_L-type_dom PF18483 31 - 250 4.6e-07 Legume lectin beta-barrel domain
Pkinase PF00069 345 - 550 2.3e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 347 - 612 6e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 828
Acc36I ACCTGC 3 cut(s) 1378, 1508, 1702
AccII CGCG 1 cut(s) 1472
AciI CCGC 4 cut(s) 509, 1112, 1620, 1757
AclWI GGATC 6 cut(s) 121, 380, 681, 835, 848, 1490
AcoI YGGCCR 1 cut(s) 1263
AcsI RAATTY 2 cut(s) 154, 816
AcuI CTGAAG 1 cut(s) 953
AcyI GRCGYC 1 cut(s) 1183
AfaI GTAC 8 cut(s) 24, 104, 226, 532, 1522, 1570, 1665, 1909
AfiI CCNNNNNNNGG 1 cut(s) 1381
AflIII ACRYGT 1 cut(s) 1818
AjuI GAANNNNNNNTTGG 4 cut(s) 932, 963, 964, 995
AleI CACNNNNGTG 1 cut(s) 1233
AluBI AGCT 8 cut(s) 407, 415, 946, 1352, 1477, 1546, 1858, 1931
AluI AGCT 8 cut(s) 407, 415, 946, 1352, 1477, 1546, 1858, 1931
Alw21I GWGCWC 2 cut(s) 417, 1548
Alw26I GTCTC 7 cut(s) 52, 336, 548, 917, 1190, 1472, 1990
AlwI GGATC 6 cut(s) 121, 380, 681, 835, 848, 1490
Ama87I CYCGRG 2 cut(s) 38, 1890
AoxI GGCC 1 cut(s) 1263
ApeKI GCWGC 2 cut(s) 1802, 1928
ApoI RAATTY 2 cut(s) 154, 816
ArsI GACNNNNNNTTYG 4 cut(s) 160, 192, 422, 454
AseI ATTAAT 1 cut(s) 609
AspS9I GGNCC 2 cut(s) 730, 983
AsuHPI GGTGA 5 cut(s) 1126, 1247, 1469, 1530, 1934
AsuII TTCGAA 1 cut(s) 538
AvaI CYCGRG 2 cut(s) 38, 1890
AvaII GGWCC 2 cut(s) 730, 983
BaeI ACNNNNGTAYC 4 cut(s) 411, 444, 1029, 1062
BalI TGGCCA 1 cut(s) 1265
BamHI GGATCC 1 cut(s) 840
BanII GRGCYC 2 cut(s) 417, 1548
BarI GAAGNNNNNNTAC 2 cut(s) 217, 249
BauI CACGAG 1 cut(s) 1641
Bbv12I GWGCWC 2 cut(s) 417, 1548
BbvI GCAGC 2 cut(s) 1814, 1940
BccI CCATC 6 cut(s) 753, 1114, 1302, 1654, 1814, 1961
BciVI GTATCC 1 cut(s) 1030
BclI TGATCA 2 cut(s) 141, 1720
BcoDI GTCTC 7 cut(s) 52, 336, 548, 917, 1190, 1472, 1990
BfaI CTAG 3 cut(s) 1607, 1682, 1824
BfmI CTRYAG 2 cut(s) 723, 1222
BfuAI ACCTGC 3 cut(s) 1378, 1508, 1702
BfuI GTATCC 1 cut(s) 1030
BglII AGATCT 2 cut(s) 325, 1006
BisI GCNGC 3 cut(s) 1758, 1803, 1929
BlpI GCTNAGC 1 cut(s) 1854
BlsI GCNGC 3 cut(s) 1759, 1804, 1930
Bme18I GGWCC 2 cut(s) 730, 983
BmeT110I CYCGRG 2 cut(s) 38, 1890
BmgT120I GGNCC 2 cut(s) 730, 983
BmiI GGNNCC 2 cut(s) 731, 842
BmrI ACTGGG 2 cut(s) 976, 1314
BmsI GCATC 4 cut(s) 442, 749, 1593, 1655
BmuI ACTGGG 2 cut(s) 976, 1314
BoxI GACNNNNGTC 1 cut(s) 1464
BpmI CTGGAG 2 cut(s) 771, 1923
Bpu10I CCTNAGC 2 cut(s) 639, 1542
Bpu1102I GCTNAGC 1 cut(s) 1854
Bpu14I TTCGAA 1 cut(s) 538
BpuEI CTTGAG 4 cut(s) 928, 980, 1120, 1534
BsaHI GRCGYC 1 cut(s) 1183
BsaI GGTCTC 2 cut(s) 52, 1472
BsaJI CCNNGG 2 cut(s) 355, 836
Bsc4I CCNNNNNNNGG 1 cut(s) 1381
Bse1I ACTGG 7 cut(s) 90, 108, 178, 971, 1091, 1309, 1796
BseDI CCNNGG 2 cut(s) 355, 836
BseGI GGATG 5 cut(s) 622, 764, 1192, 1422, 1792
BseLI CCNNNNNNNGG 1 cut(s) 1381
BseMII CTCAG 9 cut(s) 228, 928, 1032, 1155, 1201, 1514, 1533, 1868, 1932
BseNI ACTGG 7 cut(s) 90, 108, 178, 971, 1091, 1309, 1796
BseRI GAGGAG 1 cut(s) 1763
BseXI GCAGC 2 cut(s) 1814, 1940
BsgI GTGCAG 1 cut(s) 1406
Bsh1236I CGCG 1 cut(s) 1472
BshFI GGCC 1 cut(s) 1265
BsiHKAI GWGCWC 2 cut(s) 417, 1548
BsiHKCI CYCGRG 2 cut(s) 38, 1890
BslFI GGGAC 5 cut(s) 20, 620, 743, 793, 1504
BslI CCNNNNNNNGG 1 cut(s) 1381
BsmAI GTCTC 7 cut(s) 52, 336, 548, 917, 1190, 1472, 1990
BsmBI CGTCTC 1 cut(s) 1190
BsmFI GGGAC 5 cut(s) 20, 620, 743, 793, 1504
BsmI GAATGC 1 cut(s) 1450
BsnI GGCC 1 cut(s) 1265
Bso31I GGTCTC 2 cut(s) 52, 1472
BsoBI CYCGRG 2 cut(s) 38, 1890
Bsp119I TTCGAA 1 cut(s) 538
Bsp1286I GDGCHC 2 cut(s) 417, 1548
Bsp1407I TGTACA 1 cut(s) 102
Bsp1720I GCTNAGC 1 cut(s) 1854
Bsp19I CCATGG 1 cut(s) 836
Bsp68I TCGCGA 1 cut(s) 1472
BspACI CCGC 4 cut(s) 509, 1112, 1620, 1757
BspANI GGCC 1 cut(s) 1265
BspCNI CTCAG 9 cut(s) 227, 927, 1033, 1156, 1200, 1513, 1534, 1867, 1931
BspFNI CGCG 1 cut(s) 1472
BspHI TCATGA 1 cut(s) 1129
BspLI GGNNCC 2 cut(s) 731, 842
BspMAI CTGCAG 1 cut(s) 1226
BspMI ACCTGC 3 cut(s) 1378, 1508, 1702
BspPI GGATC 6 cut(s) 121, 380, 681, 835, 848, 1490
BspT104I TTCGAA 1 cut(s) 538
BspTNI GGTCTC 2 cut(s) 52, 1472
BsrGI TGTACA 1 cut(s) 102
BsrI ACTGG 7 cut(s) 90, 108, 178, 971, 1091, 1309, 1796
BssECI CCNNGG 2 cut(s) 355, 836
BssNI GRCGYC 1 cut(s) 1183
BssSI CACGAG 1 cut(s) 1641
BssT1I CCWWGG 2 cut(s) 355, 836
Bst2BI CACGAG 1 cut(s) 1641
Bst6I CTCTTC 1 cut(s) 929
BstACI GRCGYC 1 cut(s) 1183
BstAUI TGTACA 1 cut(s) 102
BstBI TTCGAA 1 cut(s) 538
BstC8I GCNNGC 3 cut(s) 1618, 1622, 1755
BstDSI CCRYGG 1 cut(s) 836
BstF5I GGATG 5 cut(s) 622, 764, 1192, 1422, 1792
BstFNI CGCG 1 cut(s) 1472
BstMAI GTCTC 7 cut(s) 52, 336, 548, 917, 1190, 1472, 1990
BstMWI GCNNNNNNNGC 1 cut(s) 1169
BstNSI RCATGY 1 cut(s) 1822
BstPAI GACNNNNGTC 1 cut(s) 1464
BstSFI CTRYAG 2 cut(s) 723, 1222
BstUI CGCG 1 cut(s) 1472
BstV1I GCAGC 2 cut(s) 1814, 1940
BstX2I RGATCY 5 cut(s) 325, 385, 673, 840, 1006
BstYI RGATCY 5 cut(s) 325, 385, 673, 840, 1006
BsuI GTATCC 1 cut(s) 1030
BsuRI GGCC 1 cut(s) 1265
BtgI CCRYGG 1 cut(s) 836
BtgZI GCGATG 1 cut(s) 1939
BtsCI GGATG 5 cut(s) 622, 764, 1192, 1422, 1792
BtsIMutI CAGTG 7 cut(s) 97, 115, 222, 562, 1098, 1852, 1971
BtuMI TCGCGA 1 cut(s) 1472
BveI ACCTGC 3 cut(s) 1378, 1508, 1702
Cac8I GCNNGC 3 cut(s) 1618, 1622, 1755
CciI TCATGA 1 cut(s) 1129
Cfr13I GGNCC 2 cut(s) 730, 983
CseI GACGC 4 cut(s) 34, 1172, 1442, 1816
Csp6I GTAC 8 cut(s) 23, 103, 225, 531, 1521, 1569, 1664, 1908
CspCI CAANNNNNGTGG 4 cut(s) 73, 108, 337, 372
CviQI GTAC 8 cut(s) 23, 103, 225, 531, 1521, 1569, 1664, 1908
EaeI YGGCCR 1 cut(s) 1263
Eam1104I CTCTTC 1 cut(s) 929
EarI CTCTTC 1 cut(s) 929
Ecl136II GAGCTC 2 cut(s) 415, 1546
Eco130I CCWWGG 2 cut(s) 355, 836
Eco24I GRGCYC 2 cut(s) 417, 1548
Eco31I GGTCTC 2 cut(s) 52, 1472
Eco32I GATATC 2 cut(s) 475, 1705
Eco47I GGWCC 2 cut(s) 730, 983
Eco53kI GAGCTC 2 cut(s) 415, 1546
Eco57I CTGAAG 1 cut(s) 953
Eco88I CYCGRG 2 cut(s) 38, 1890
EcoICRI GAGCTC 2 cut(s) 415, 1546
EcoO109I RGGNCCY 1 cut(s) 730
EcoRV GATATC 2 cut(s) 475, 1705
EcoT14I CCWWGG 2 cut(s) 355, 836
EcoT38I GRGCYC 2 cut(s) 417, 1548
ErhI CCWWGG 2 cut(s) 355, 836
Esp3I CGTCTC 1 cut(s) 1190
FalI AAGNNNNNCTT 4 cut(s) 1260, 1292, 1746, 1778
FaqI GGGAC 5 cut(s) 20, 620, 743, 793, 1504
FauI CCCGC 2 cut(s) 502, 1613
FbaI TGATCA 2 cut(s) 141, 1720
Fnu4HI GCNGC 3 cut(s) 1758, 1803, 1929
FokI GGATG 5 cut(s) 609, 771, 1179, 1409, 1779
FriOI GRGCYC 2 cut(s) 417, 1548
Fsp4HI GCNGC 3 cut(s) 1758, 1803, 1929
FspBI CTAG 3 cut(s) 1607, 1682, 1824
GluI GCNGC 3 cut(s) 1758, 1803, 1929
GsuI CTGGAG 2 cut(s) 771, 1923
HaeIII GGCC 1 cut(s) 1265
HgaI GACGC 4 cut(s) 34, 1172, 1442, 1816
Hin1I GRCGYC 1 cut(s) 1183
HincII GTYRAC 1 cut(s) 430
HindII GTYRAC 1 cut(s) 430
HphI GGTGA 5 cut(s) 1126, 1247, 1469, 1530, 1934
Hpy166II GTNNAC 7 cut(s) 105, 220, 430, 560, 596, 1238, 1523
Hpy188I TCNGA 9 cut(s) 57, 141, 146, 493, 1190, 1503, 1579, 1770, 1811
Hpy8I GTNNAC 7 cut(s) 105, 220, 430, 560, 596, 1238, 1523
Hpy99I CGWCG 1 cut(s) 50
HpyAV CCTTC 4 cut(s) 247, 818, 1436, 1978
HpyCH4IV ACGT 3 cut(s) 764, 1101, 1347
HpyCH4V TGCA 9 cut(s) 571, 740, 1012, 1172, 1224, 1372, 1387, 1711, 1885
HpyF10VI GCNNNNNNNGC 1 cut(s) 1169
HpySE526I ACGT 3 cut(s) 764, 1101, 1347
Hsp92I GRCGYC 1 cut(s) 1183
Ksp22I TGATCA 2 cut(s) 141, 1720
LmnI GCTCC 3 cut(s) 372, 1750, 1963
Lsp1109I GCAGC 2 cut(s) 1814, 1940
LweI GCATC 4 cut(s) 442, 749, 1593, 1655
MaeI CTAG 3 cut(s) 1607, 1682, 1824
MaeII ACGT 3 cut(s) 764, 1101, 1347
MaeIII GTNAC 3 cut(s) 613, 1320, 1457
MboII GAAGA 6 cut(s) 22, 225, 946, 952, 1129, 1712
MflI RGATCY 5 cut(s) 325, 385, 673, 840, 1006
MhlI GDGCHC 2 cut(s) 417, 1548
MlsI TGGCCA 1 cut(s) 1265
MluCI AATT 5 cut(s) 154, 297, 437, 816, 1835
MluNI TGGCCA 1 cut(s) 1265
MlyI GAGTC 6 cut(s) 141, 856, 897, 919, 1127, 1952
MmeI TCCRAC 3 cut(s) 341, 688, 1122
Mox20I TGGCCA 1 cut(s) 1265
MscI TGGCCA 1 cut(s) 1265
MseI TTAA 4 cut(s) 552, 609, 875, 1410
MslI CAYNNNNRTG 1 cut(s) 1233
Msp20I TGGCCA 1 cut(s) 1265
MspA1I CMGCKG 1 cut(s) 1931
Mva1269I GAATGC 1 cut(s) 1450
MvnI CGCG 1 cut(s) 1472
MwoI GCNNNNNNNGC 1 cut(s) 1169
NcoI CCATGG 1 cut(s) 836
NlaIV GGNNCC 2 cut(s) 731, 842
NmuCI GTSAC 3 cut(s) 613, 1320, 1457
NruI TCGCGA 1 cut(s) 1472
NspI RCATGY 1 cut(s) 1822
NspV TTCGAA 1 cut(s) 538
OliI CACNNNNGTG 1 cut(s) 1233
PaeR7I CTCGAG 1 cut(s) 1890
PagI TCATGA 1 cut(s) 1129
PciI ACATGT 1 cut(s) 1818
PctI GAATGC 1 cut(s) 1450
PfeI GAWTC 7 cut(s) 204, 649, 711, 1309, 1329, 1934, 1976
PkrI GCNGC 3 cut(s) 1759, 1804, 1930
PleI GAGTC 6 cut(s) 141, 855, 896, 918, 1127, 1951
PpsI GAGTC 6 cut(s) 141, 855, 896, 918, 1127, 1951
PpuMI RGGWCCY 1 cut(s) 730
PscI ACATGT 1 cut(s) 1818
PshAI GACNNNNGTC 1 cut(s) 1464
PshBI ATTAAT 1 cut(s) 609
PsiI TTATAA 1 cut(s) 828
Psp124BI GAGCTC 2 cut(s) 417, 1548
Psp5II RGGWCCY 1 cut(s) 730
PspN4I GGNNCC 2 cut(s) 731, 842
PspPI GGNCC 2 cut(s) 730, 983
PspPPI RGGWCCY 1 cut(s) 730
PstI CTGCAG 1 cut(s) 1226
PsuI RGATCY 5 cut(s) 325, 385, 673, 840, 1006
PvuII CAGCTG 1 cut(s) 1931
RruI TCGCGA 1 cut(s) 1472
RsaI GTAC 8 cut(s) 24, 104, 226, 532, 1522, 1570, 1665, 1909
RsaNI GTAC 8 cut(s) 23, 103, 225, 531, 1521, 1569, 1664, 1908
RseI CAYNNNNRTG 1 cut(s) 1233
SacI GAGCTC 2 cut(s) 417, 1548
SaqAI TTAA 4 cut(s) 552, 609, 875, 1410
SatI GCNGC 3 cut(s) 1758, 1803, 1929
Sau96I GGNCC 2 cut(s) 730, 983
SchI GAGTC 6 cut(s) 141, 856, 897, 919, 1127, 1952
SduI GDGCHC 2 cut(s) 417, 1548
SfaNI GCATC 4 cut(s) 442, 749, 1593, 1655
SfcI CTRYAG 2 cut(s) 723, 1222
Sfr274I CTCGAG 1 cut(s) 1890
SfuI TTCGAA 1 cut(s) 538
SinI GGWCC 2 cut(s) 730, 983
SlaI CTCGAG 1 cut(s) 1890
SmiMI CAYNNNNRTG 1 cut(s) 1233
SmlI CTYRAG 5 cut(s) 907, 959, 1135, 1549, 1890
SmoI CTYRAG 5 cut(s) 907, 959, 1135, 1549, 1890
Sse9I AATT 5 cut(s) 154, 297, 437, 816, 1835
SsiI CCGC 4 cut(s) 509, 1112, 1620, 1757
SspMI CTAG 3 cut(s) 1607, 1682, 1824
SstI GAGCTC 2 cut(s) 417, 1548
StyI CCWWGG 2 cut(s) 355, 836
TaiI ACGT 3 cut(s) 767, 1104, 1350
TaqI TCGA 6 cut(s) 202, 417, 441, 538, 1891, 1903
TasI AATT 5 cut(s) 154, 297, 437, 816, 1835
TatI WGTACW 2 cut(s) 102, 1568
TauI GCSGC 1 cut(s) 1760
TfiI GAWTC 7 cut(s) 204, 649, 711, 1309, 1329, 1934, 1976
Tru1I TTAA 4 cut(s) 552, 609, 875, 1410
Tru9I TTAA 4 cut(s) 552, 609, 875, 1410
TscAI CASTG 7 cut(s) 97, 115, 222, 562, 1098, 1852, 1978
TseFI GTSAC 3 cut(s) 613, 1320, 1457
TseI GCWGC 2 cut(s) 1802, 1928
Tsp45I GTSAC 3 cut(s) 613, 1320, 1457
TspDTI ATGAA 6 cut(s) 260, 611, 788, 1457, 1713, 1889
TspRI CASTG 7 cut(s) 97, 115, 222, 562, 1098, 1852, 1978
VpaK11BI GGWCC 2 cut(s) 730, 983
VspI ATTAAT 1 cut(s) 609
XapI RAATTY 2 cut(s) 154, 816
XceI RCATGY 1 cut(s) 1822
XcmI CCANNNNNNNNNTGG 2 cut(s) 851, 1301
XhoI CTCGAG 1 cut(s) 1890
XspI CTAG 3 cut(s) 1607, 1682, 1824
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.