AT1G74490
ERF Family

belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
N/A
Physical Location & Seq
Reverse (-)
27994652 .. 27996794
2143 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G74490.1

Sequence Viewer

Length: 1200 bp
ATGGGGAATTGCTTTGAATCTTGTTCGAAGAGAAATGATAATGAGGACGATAATCTTGACTCCTCAGTGAACTCGAAACCCTTTTCACGAGCTAATAGTGATACTGGAAGAAGCTCGAATCTGTCATATCCATGGAGTTTAAAACCTCTCATTACAAGAAAATGTGAAGCTATCAGTGCTCTACCTCCACCGCACAAAGAAGGAGACATAATGCATTCTCAATATTTGAAGTCCTTTACCTTAGATGAGCTAAAGAATGCTACAGGAAACTTCTGTCCAGAGAGTCTAATTGGAGAAGGAGGGTTCGGGTTTGTTCACAAAGGCTGTATTAATGGTGGACCTGGAATCGAACTCGCGGTTGCTGTAAAGAAGCTTAAAACCGAAGGGCTTCAGGGACATAAGGAGTGGCTGAGGGAAGTGAATTATCTTGGAAGATTGCATCATCCGAATCTAGTGAAACTGATTGGTTACTCCTTGGAAAACGAACACCGCCTTTTGGTTTACGAGCATTTGCCTAATGGTAGTCTTGAGAATCATTTGTTTGAAAGGAGTTCCAGTGTGCTTTCTTGGTCACTGCGTATGAAAGTCGCGATTGGTGCTGCTCGAGGGCTATGTTTTTTGCATGAAGCTAATGACCAAGTCATTTACAGAGACTTCAAAGCAGCAAATATCCTTCTCGATTCAGGATTCAACGCCAAACTCTCAGACTTTGGATTGGCCAAAGAAGGTCCAAAAGATAACCGTTCACATGTGACAACAGAAGTCATGGGTACAGAAGGTTACGCAGCTCCAGAGTATCTAGCAACAGGTCATCTAACAACGAAATGTGATGTCTACAGCTTCGGTGTAGTGTTGCTAGAGATTCTCTCTGGGCGTCGAGTCATAGACAAATCCAAATCACGGGAAGAAGAAAACCTAGTGGATTGGGCAACACCTTACTTGCGAGACAAGCGAAAAGTGTTTAGGATAATGGACACAAAGCTTGTGGGTCAATACCCTCAGAAAGCAGCGTTTATGATGTCTTTCTTGGCTTTACAATGTATCGGAGATGTTAAGGTTAGACCATCGATGCTTGAGGTCGTATCTCTTCTTGAGAAAGTTCCAATTCCAAGACATAGAAAGAGTAGAAGCAAAGGTTTTGCTTGTACTAACTCTGCTTCTATGCCTTCAAAACGGTTTCTCAGACATCCTAAGGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

399

Amino Acids

44.65

Weight (kDa)

8.98

Isoelectric Point (pI)

39.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 94 - 307 2.8e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 96 - 301 3.5e-42 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 834
AccII CGCG 2 cut(s) 356, 590
AciI CCGC 3 cut(s) 191, 356, 490
AcoI YGGCCR 1 cut(s) 717
AcuI CTGAAG 1 cut(s) 374
AcyI GRCGYC 1 cut(s) 874
AfaI GTAC 2 cut(s) 772, 1147
AfiI CCNNNNNNNGG 2 cut(s) 496, 900
AflIII ACRYGT 1 cut(s) 748
AgsI TTSAA 6 cut(s) 17, 229, 545, 658, 691, 1170
AjnI CCWGG 1 cut(s) 340
AluBI AGCT 9 cut(s) 92, 114, 170, 250, 373, 629, 788, 840, 982
AluI AGCT 9 cut(s) 92, 114, 170, 250, 373, 629, 788, 840, 982
Alw21I GWGCWC 1 cut(s) 181
Alw26I GTCTC 3 cut(s) 198, 645, 939
Ama87I CYCGRG 1 cut(s) 603
AoxI GGCC 1 cut(s) 717
ApeKI GCWGC 4 cut(s) 599, 662, 785, 1007
AseI ATTAAT 1 cut(s) 330
Asp700I GAANNNNTTC 1 cut(s) 387
AspS9I GGNCC 2 cut(s) 338, 728
AsuII TTCGAA 1 cut(s) 26
AvaI CYCGRG 1 cut(s) 603
AvaII GGWCC 2 cut(s) 338, 728
AxyI CCTNAGG 1 cut(s) 1191
BalI TGGCCA 1 cut(s) 719
BauI CACGAG 1 cut(s) 87
Bbv12I GWGCWC 1 cut(s) 181
BbvCI CCTCAGC 1 cut(s) 410
BbvI GCAGC 4 cut(s) 586, 674, 797, 1019
BccI CCATC 1 cut(s) 1072
BciT130I CCWGG 1 cut(s) 342
BcoDI GTCTC 3 cut(s) 198, 645, 939
BfaI CTAG 4 cut(s) 452, 800, 857, 917
BfmI CTRYAG 2 cut(s) 261, 835
BisI GCNGC 4 cut(s) 600, 663, 786, 1008
BlsI GCNGC 4 cut(s) 601, 664, 787, 1009
Bme1390I CCNGG 1 cut(s) 342
Bme18I GGWCC 2 cut(s) 338, 728
BmeT110I CYCGRG 1 cut(s) 603
BmgT120I GGNCC 2 cut(s) 338, 728
BmrFI CCNGG 1 cut(s) 342
BmsI GCATC 2 cut(s) 448, 1059
BplI GAGNNNNNCTC 2 cut(s) 851, 883
BpmI CTGGAG 1 cut(s) 774
Bpu10I CCTNAGC 1 cut(s) 410
Bpu14I TTCGAA 1 cut(s) 26
BpuEI CTTGAG 3 cut(s) 548, 1094, 1112
Bsa29I ATCGAT 1 cut(s) 1067
BsaHI GRCGYC 1 cut(s) 874
BsaJI CCNNGG 2 cut(s) 131, 474
BsaXI ACNNNNNCTCC 2 cut(s) 541, 571
Bsc4I CCNNNNNNNGG 2 cut(s) 496, 900
Bse1I ACTGG 2 cut(s) 109, 555
Bse21I CCTNAGG 1 cut(s) 1191
BseBI CCWGG 1 cut(s) 342
BseCI ATCGAT 1 cut(s) 1067
BseDI CCNNGG 2 cut(s) 131, 474
BseGI GGATG 2 cut(s) 442, 1186
BseLI CCNNNNNNNGG 2 cut(s) 496, 900
BseMII CTCAG 5 cut(s) 78, 401, 717, 1013, 1195
BseNI ACTGG 2 cut(s) 109, 555
BseRI GAGGAG 1 cut(s) 52
BseXI GCAGC 4 cut(s) 586, 674, 797, 1019
Bsh1236I CGCG 2 cut(s) 356, 590
BshFI GGCC 1 cut(s) 719
BshVI ATCGAT 1 cut(s) 1067
BsiHKAI GWGCWC 1 cut(s) 181
BsiHKCI CYCGRG 1 cut(s) 603
BslFI GGGAC 1 cut(s) 408
BslI CCNNNNNNNGG 2 cut(s) 496, 900
BsmAI GTCTC 3 cut(s) 198, 645, 939
BsmFI GGGAC 1 cut(s) 408
BsmI GAATGC 2 cut(s) 214, 262
BsnI GGCC 1 cut(s) 719
BsoBI CYCGRG 1 cut(s) 603
Bsp119I TTCGAA 1 cut(s) 26
Bsp1286I GDGCHC 1 cut(s) 181
Bsp19I CCATGG 1 cut(s) 131
Bsp68I TCGCGA 1 cut(s) 590
BspACI CCGC 3 cut(s) 191, 356, 490
BspANI GGCC 1 cut(s) 719
BspCNI CTCAG 5 cut(s) 77, 402, 716, 1012, 1194
BspDI ATCGAT 1 cut(s) 1067
BspFNI CGCG 2 cut(s) 356, 590
BspT104I TTCGAA 1 cut(s) 26
BsrI ACTGG 2 cut(s) 109, 555
BssECI CCNNGG 2 cut(s) 131, 474
BssNI GRCGYC 1 cut(s) 874
BssSI CACGAG 1 cut(s) 87
BssT1I CCWWGG 2 cut(s) 131, 474
Bst2BI CACGAG 1 cut(s) 87
Bst2UI CCWGG 1 cut(s) 342
Bst4CI ACNGT 2 cut(s) 743, 1176
Bst6I CTCTTC 2 cut(s) 23, 1092
BstACI GRCGYC 1 cut(s) 874
BstBI TTCGAA 1 cut(s) 26
BstDEI CTNAG 7 cut(s) 64, 241, 410, 703, 999, 1181, 1191
BstDSI CCRYGG 1 cut(s) 131
BstF5I GGATG 2 cut(s) 442, 1186
BstFNI CGCG 2 cut(s) 356, 590
BstMAI GTCTC 3 cut(s) 198, 645, 939
BstMWI GCNNNNNNNGC 3 cut(s) 176, 596, 949
BstNI CCWGG 1 cut(s) 342
BstNSI RCATGY 1 cut(s) 752
BstSCI CCNGG 1 cut(s) 340
BstSFI CTRYAG 2 cut(s) 261, 835
BstUI CGCG 2 cut(s) 356, 590
BstV1I GCAGC 4 cut(s) 586, 674, 797, 1019
Bsu15I ATCGAT 1 cut(s) 1067
Bsu36I CCTNAGG 1 cut(s) 1191
BsuRI GGCC 1 cut(s) 719
BsuTUI ATCGAT 1 cut(s) 1067
BtgI CCRYGG 1 cut(s) 131
BtsCI GGATG 2 cut(s) 442, 1186
BtsI GCAGTG 1 cut(s) 572
BtsIMutI CAGTG 4 cut(s) 72, 181, 562, 572
BtuMI TCGCGA 1 cut(s) 590
Cfr13I GGNCC 2 cut(s) 338, 728
ClaI ATCGAT 1 cut(s) 1067
CseI GACGC 1 cut(s) 863
Csp6I GTAC 2 cut(s) 771, 1146
CspCI CAANNNNNGTGG 2 cut(s) 966, 1001
CviAII CATG 4 cut(s) 132, 623, 749, 766
CviQI GTAC 2 cut(s) 771, 1146
DdeI CTNAG 7 cut(s) 64, 241, 410, 703, 999, 1181, 1191
DraI TTTAAA 1 cut(s) 141
EaeI YGGCCR 1 cut(s) 717
Eam1104I CTCTTC 2 cut(s) 23, 1092
EarI CTCTTC 2 cut(s) 23, 1092
Eco130I CCWWGG 2 cut(s) 131, 474
Eco47I GGWCC 2 cut(s) 338, 728
Eco57I CTGAAG 1 cut(s) 374
Eco81I CCTNAGG 1 cut(s) 1191
Eco88I CYCGRG 1 cut(s) 603
EcoRII CCWGG 1 cut(s) 340
EcoT14I CCWWGG 2 cut(s) 131, 474
EcoT22I ATGCAT 1 cut(s) 216
ErhI CCWWGG 2 cut(s) 131, 474
FaeI CATG 4 cut(s) 135, 626, 752, 769
FaqI GGGAC 1 cut(s) 408
FatI CATG 4 cut(s) 131, 622, 748, 765
FblI GTMKAC 1 cut(s) 834
Fnu4HI GCNGC 4 cut(s) 600, 663, 786, 1008
FokI GGATG 2 cut(s) 429, 1173
Fsp4HI GCNGC 4 cut(s) 600, 663, 786, 1008
FspBI CTAG 4 cut(s) 452, 800, 857, 917
GluI GCNGC 4 cut(s) 600, 663, 786, 1008
GsuI CTGGAG 1 cut(s) 774
HaeIII GGCC 1 cut(s) 719
HgaI GACGC 1 cut(s) 863
Hin1I GRCGYC 1 cut(s) 874
Hin1II CATG 4 cut(s) 135, 626, 752, 769
HindIII AAGCTT 2 cut(s) 371, 980
Hpy166II GTNNAC 6 cut(s) 70, 316, 338, 502, 746, 835
Hpy188I TCNGA 5 cut(s) 447, 706, 1002, 1046, 1184
Hpy188III TCNNGA 9 cut(s) 56, 87, 278, 527, 589, 677, 684, 791, 1091
Hpy8I GTNNAC 6 cut(s) 70, 316, 338, 502, 746, 835
Hpy99I CGWCG 1 cut(s) 879
HpyAV CCTTC 7 cut(s) 194, 290, 377, 683, 719, 770, 1176
HpyCH4III ACNGT 2 cut(s) 743, 1176
HpyCH4V TGCA 3 cut(s) 214, 439, 622
HpyF10VI GCNNNNNNNGC 3 cut(s) 176, 596, 949
HpyF3I CTNAG 7 cut(s) 64, 241, 410, 703, 999, 1181, 1191
Hsp92I GRCGYC 1 cut(s) 874
Hsp92II CATG 4 cut(s) 135, 626, 752, 769
LmnI GCTCC 1 cut(s) 793
Lsp1109I GCAGC 4 cut(s) 586, 674, 797, 1019
LweI GCATC 2 cut(s) 448, 1059
MaeI CTAG 4 cut(s) 452, 800, 857, 917
MaeIII GTNAC 4 cut(s) 467, 570, 751, 779
MboII GAAGA 6 cut(s) 40, 120, 444, 917, 920, 1079
MhlI GDGCHC 1 cut(s) 181
MlsI TGGCCA 1 cut(s) 719
MluCI AATT 4 cut(s) 7, 288, 421, 1104
MluNI TGGCCA 1 cut(s) 719
MlyI GAGTC 3 cut(s) 53, 292, 888
MnlI CCTC 9 cut(s) 37, 73, 156, 195, 293, 405, 599, 1008, 1069
Mox20I TGGCCA 1 cut(s) 719
Mph1103I ATGCAT 1 cut(s) 216
MroXI GAANNNNTTC 1 cut(s) 387
MscI TGGCCA 1 cut(s) 719
MseI TTAA 4 cut(s) 140, 330, 375, 1053
MslI CAYNNNNRTG 1 cut(s) 130
Msp20I TGGCCA 1 cut(s) 719
MspR9I CCNGG 1 cut(s) 342
Mva1269I GAATGC 2 cut(s) 214, 262
MvaI CCWGG 1 cut(s) 342
MvnI CGCG 2 cut(s) 356, 590
MwoI GCNNNNNNNGC 3 cut(s) 176, 596, 949
NcoI CCATGG 1 cut(s) 131
NlaIII CATG 4 cut(s) 135, 626, 752, 769
NmuCI GTSAC 2 cut(s) 570, 751
NruI TCGCGA 1 cut(s) 590
NsiI ATGCAT 1 cut(s) 216
NspI RCATGY 1 cut(s) 752
NspV TTCGAA 1 cut(s) 26
PaeR7I CTCGAG 1 cut(s) 603
PciI ACATGT 1 cut(s) 748
PctI GAATGC 2 cut(s) 214, 262
PdmI GAANNNNTTC 1 cut(s) 387
PfeI GAWTC 8 cut(s) 17, 118, 345, 448, 532, 680, 687, 862
PflFI GACNNNGTC 1 cut(s) 638
PkrI GCNGC 4 cut(s) 601, 664, 787, 1009
PleI GAGTC 3 cut(s) 53, 291, 887
PpsI GAGTC 3 cut(s) 53, 291, 887
PscI ACATGT 1 cut(s) 748
PshBI ATTAAT 1 cut(s) 330
Psp6I CCWGG 1 cut(s) 340
PspGI CCWGG 1 cut(s) 340
PspPI GGNCC 2 cut(s) 338, 728
PspXI VCTCGAGB 1 cut(s) 603
PsyI GACNNNGTC 1 cut(s) 638
RruI TCGCGA 1 cut(s) 590
RsaI GTAC 2 cut(s) 772, 1147
RsaNI GTAC 2 cut(s) 771, 1146
RseI CAYNNNNRTG 1 cut(s) 130
SaqAI TTAA 4 cut(s) 140, 330, 375, 1053
SatI GCNGC 4 cut(s) 600, 663, 786, 1008
Sau96I GGNCC 2 cut(s) 338, 728
SchI GAGTC 3 cut(s) 53, 292, 888
ScrFI CCNGG 1 cut(s) 342
SduI GDGCHC 1 cut(s) 181
SfaNI GCATC 2 cut(s) 448, 1059
SfcI CTRYAG 2 cut(s) 261, 835
Sfr274I CTCGAG 1 cut(s) 603
SfuI TTCGAA 1 cut(s) 26
SinI GGWCC 2 cut(s) 338, 728
SlaI CTCGAG 1 cut(s) 603
SmiMI CAYNNNNRTG 1 cut(s) 130
SmlI CTYRAG 4 cut(s) 527, 603, 1073, 1091
SmoI CTYRAG 4 cut(s) 527, 603, 1073, 1091
Sse9I AATT 4 cut(s) 7, 288, 421, 1104
SsiI CCGC 3 cut(s) 191, 356, 490
SspI AATATT 1 cut(s) 224
SspMI CTAG 4 cut(s) 452, 800, 857, 917
StyD4I CCNGG 1 cut(s) 340
StyI CCWWGG 2 cut(s) 131, 474
TaaI ACNGT 2 cut(s) 743, 1176
TaqI TCGA 8 cut(s) 26, 74, 116, 348, 604, 678, 877, 1067
TasI AATT 4 cut(s) 7, 288, 421, 1104
TatI WGTACW 1 cut(s) 1145
TfiI GAWTC 8 cut(s) 17, 118, 345, 448, 532, 680, 687, 862
Tru1I TTAA 4 cut(s) 140, 330, 375, 1053
Tru9I TTAA 4 cut(s) 140, 330, 375, 1053
TscAI CASTG 4 cut(s) 72, 181, 562, 579
TseFI GTSAC 2 cut(s) 570, 751
TseI GCWGC 4 cut(s) 599, 662, 785, 1007
Tsp45I GTSAC 2 cut(s) 570, 751
TspDTI ATGAA 2 cut(s) 596, 639
TspRI CASTG 4 cut(s) 72, 181, 562, 579
Tth111I GACNNNGTC 1 cut(s) 638
VpaK11BI GGWCC 2 cut(s) 338, 728
VspI ATTAAT 1 cut(s) 330
XceI RCATGY 1 cut(s) 752
XhoI CTCGAG 1 cut(s) 603
XmiI GTMKAC 1 cut(s) 834
XmnI GAANNNNTTC 1 cut(s) 387
XspI CTAG 4 cut(s) 452, 800, 857, 917
Zsp2I ATGCAT 1 cut(s) 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.