AT3G52530
NAC Family

Inactive serine threonine-protein kinase At1g67470-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
N/A
Physical Location & Seq
Reverse (-)
19484567 .. 19486130
1564 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G52530.1

Sequence Viewer

Length: 1056 bp
ATGAAGGGTTTTTTCAAAACGGAGAGTGAGACAAGGAAACATTCAGACAAGAACGGGAGCTTGTTGCACGAGGAGCTAATCGCGTGTTCAGATGGTAAATACAATCCAATCCGAATGTTTTCGTCTGATCAAATTCTTAAAGCAACAAACAACTTCGACGCAGATCATATCATAGCAAAAGATAGATTTATATGGTACAAGGGTACTATAGAAGAACGGAGAGTACTCATCAAGAAATGGGAAGGAGATTATGTCCTTTTTTCTTCTCCTGAAAATGTTTACCGCGATATAGCGGTTTTGTCGATGATGAGCAGTCACAAGAACGTTTTGAAGCTGTTGGGTTGCTGTGTGGAGTTTTATAAACCGGTTCTGGTTTGTGAGTTAGCAGAGAAAGGACCTCTAAAACTCGAGGACATGGACGGTACTCCGTTACCTTGGAGTGCAAGGTTAAAGATCGGGAAGGATATTGCAAATGCGGTAGCTTATCTTCACACAGCTTTTCCTCGAGTCATAATCAACAGGGATGTAAGACCTCAAAACATATTCTTGGACGAAGATGGGACTGCGAAGCTCTCTTCTTTCTGTTTGAGAATATCGATCCCTGAGGGAGAATCATCTGTGTATGATGATAAAGTAGTTTATGGGGTATCCGTTGATCCGGAGTATAATGGAACAGGTTTAGTTTCCGAAAAGTTTGATGTCTATAGCTTTGGAGTTACAATGCTGTTTCTTCTAGGCGGAGAGCTTGGCCTAACCTGGTTATCTGCTATCATTGGGGAGTTTGGATTCCCATTTCCTGGTTGTGGTGAAGAATTAGCGGATCAGTTCATGTATGTCATAGATTCAAACATATGGAACGGTGAGAGTGAAGCATCTGCTGTGCAAGTGGAAACGTTTTTTGGGCTTGCTTTGAGATGTATCAGATTCTGGCCAGGACAAGACGTGCTTACAATGATCGATGTCGCCAAAGAGCTTAAGGGGATAGAAGAACTTTTCAAAGCGTCTTCATCCGAACAGGACAAAGAACAAATTGATCAAGTCAACTACTCTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase_Tyr

Protein Analysis

351

Amino Acids

39.54

Weight (kDa)

4.86

Isoelectric Point (pI)

34.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 70 - 242 9e-20 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 74 - 248 1.4e-17 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 360
AccB7I CCANNNNNTGG 1 cut(s) 797
AccII CGCG 2 cut(s) 83, 285
AccIII TCCGGA 1 cut(s) 658
AciI CCGC 5 cut(s) 283, 293, 476, 738, 818
AclI AACGTT 2 cut(s) 324, 893
AclWI GGATC 3 cut(s) 592, 650, 828
AcoI YGGCCR 1 cut(s) 929
AcsI RAATTY 1 cut(s) 132
AfaI GTAC 4 cut(s) 197, 205, 225, 424
AfiI CCNNNNNNNGG 2 cut(s) 797, 803
AflII CTTAAG 1 cut(s) 974
AgeI ACCGGT 1 cut(s) 364
AgsI TTSAA 4 cut(s) 16, 331, 846, 997
AjiI CACGTC 1 cut(s) 943
AjnI CCWGG 3 cut(s) 755, 796, 931
AjuI GAANNNNNNNTTGG 2 cut(s) 882, 914
AluBI AGCT 9 cut(s) 60, 76, 334, 482, 497, 571, 708, 745, 973
AluI AGCT 9 cut(s) 60, 76, 334, 482, 497, 571, 708, 745, 973
Alw26I GTCTC 1 cut(s) 23
AlwI GGATC 3 cut(s) 592, 650, 828
AlwNI CAGNNNCTG 1 cut(s) 927
Ama87I CYCGRG 2 cut(s) 407, 504
Aor13HI TCCGGA 1 cut(s) 658
AoxI GGCC 2 cut(s) 748, 929
ApoI RAATTY 1 cut(s) 132
AsiGI ACCGGT 1 cut(s) 364
Asp700I GAANNNNTTC 1 cut(s) 118
AspS9I GGNCC 1 cut(s) 395
AsuHPI GGTGA 2 cut(s) 818, 872
AvaI CYCGRG 2 cut(s) 407, 504
AvaII GGWCC 1 cut(s) 395
AxyI CCTNAGG 1 cut(s) 603
BaeI ACNNNNGTAYC 2 cut(s) 187, 220
BalI TGGCCA 1 cut(s) 931
BarI GAAGNNNNNNTAC 2 cut(s) 471, 503
BauI CACGAG 1 cut(s) 68
BbsI GAAGAC 1 cut(s) 996
BccI CCATC 2 cut(s) 86, 551
BciT130I CCWGG 3 cut(s) 757, 798, 933
BciVI GTATCC 1 cut(s) 658
BclI TGATCA 2 cut(s) 127, 1033
BcoDI GTCTC 1 cut(s) 23
BfaI CTAG 1 cut(s) 734
BfmI CTRYAG 2 cut(s) 207, 703
BfrI CTTAAG 1 cut(s) 974
BfuI GTATCC 1 cut(s) 658
BmcAI AGTACT 1 cut(s) 225
Bme1390I CCNGG 3 cut(s) 757, 798, 933
Bme18I GGWCC 1 cut(s) 395
BmeT110I CYCGRG 2 cut(s) 407, 504
BmgBI CACGTC 1 cut(s) 943
BmgT120I GGNCC 1 cut(s) 395
BmrFI CCNGG 3 cut(s) 757, 798, 933
BmsI GCATC 1 cut(s) 881
BpiI GAAGAC 1 cut(s) 996
Bsa29I ATCGAT 2 cut(s) 596, 957
BsaJI CCNNGG 1 cut(s) 434
BsaWI WCCGGW 2 cut(s) 364, 658
BsaXI ACNNNNNCTCC 2 cut(s) 237, 267
Bsc4I CCNNNNNNNGG 2 cut(s) 797, 803
Bse118I RCCGGY 1 cut(s) 364
Bse21I CCTNAGG 1 cut(s) 603
BseAI TCCGGA 1 cut(s) 658
BseBI CCWGG 3 cut(s) 757, 798, 933
BseCI ATCGAT 2 cut(s) 596, 957
BseDI CCNNGG 1 cut(s) 434
BseGI GGATG 2 cut(s) 529, 1007
BseLI CCNNNNNNNGG 2 cut(s) 797, 803
BseMII CTCAG 1 cut(s) 594
BseRI GAGGAG 1 cut(s) 86
Bsh1236I CGCG 2 cut(s) 83, 285
BshFI GGCC 2 cut(s) 750, 931
BshTI ACCGGT 1 cut(s) 364
BshVI ATCGAT 2 cut(s) 596, 957
BsiHKCI CYCGRG 2 cut(s) 407, 504
BsiSI CCGG 2 cut(s) 365, 659
BslFI GGGAC 1 cut(s) 574
BslI CCNNNNNNNGG 2 cut(s) 797, 803
BsmAI GTCTC 1 cut(s) 23
BsmFI GGGAC 1 cut(s) 574
BsnI GGCC 2 cut(s) 750, 931
BsoBI CYCGRG 2 cut(s) 407, 504
Bsp13I TCCGGA 1 cut(s) 658
Bsp143I GATC 8 cut(s) 127, 163, 453, 597, 655, 820, 954, 1033
BspACI CCGC 5 cut(s) 283, 293, 476, 738, 818
BspANI GGCC 2 cut(s) 750, 931
BspCNI CTCAG 1 cut(s) 595
BspDI ATCGAT 2 cut(s) 596, 957
BspEI TCCGGA 1 cut(s) 658
BspFNI CGCG 2 cut(s) 83, 285
BspPI GGATC 3 cut(s) 592, 650, 828
BspTI CTTAAG 1 cut(s) 974
BsrFI RCCGGY 1 cut(s) 364
BssAI RCCGGY 1 cut(s) 364
BssECI CCNNGG 1 cut(s) 434
BssMI GATC 8 cut(s) 127, 163, 453, 597, 655, 820, 954, 1033
BssSI CACGAG 1 cut(s) 68
BssT1I CCWWGG 1 cut(s) 434
Bst2BI CACGAG 1 cut(s) 68
Bst2UI CCWGG 3 cut(s) 757, 798, 933
Bst4CI ACNGT 2 cut(s) 422, 860
Bst6I CTCTTC 1 cut(s) 580
BstAFI CTTAAG 1 cut(s) 974
BstC8I GCNNGC 1 cut(s) 906
BstDEI CTNAG 1 cut(s) 603
BstF5I GGATG 2 cut(s) 529, 1007
BstFNI CGCG 2 cut(s) 83, 285
BstKTI GATC 8 cut(s) 130, 166, 456, 600, 658, 823, 957, 1036
BstMAI GTCTC 1 cut(s) 23
BstMBI GATC 8 cut(s) 127, 163, 453, 597, 655, 820, 954, 1033
BstMWI GCNNNNNNNGC 1 cut(s) 73
BstNI CCWGG 3 cut(s) 757, 798, 933
BstSCI CCNGG 3 cut(s) 755, 796, 931
BstSFI CTRYAG 2 cut(s) 207, 703
BstUI CGCG 2 cut(s) 83, 285
BstV2I GAAGAC 1 cut(s) 996
Bsu15I ATCGAT 2 cut(s) 596, 957
Bsu36I CCTNAGG 1 cut(s) 603
BsuI GTATCC 1 cut(s) 658
BsuRI GGCC 2 cut(s) 750, 931
BsuTUI ATCGAT 2 cut(s) 596, 957
BtrI CACGTC 1 cut(s) 943
BtsCI GGATG 2 cut(s) 529, 1007
Cac8I GCNNGC 1 cut(s) 906
CaiI CAGNNNCTG 1 cut(s) 927
Cfr10I RCCGGY 1 cut(s) 364
Cfr13I GGNCC 1 cut(s) 395
ClaI ATCGAT 2 cut(s) 596, 957
CseI GACGC 2 cut(s) 167, 990
CsiI ACCWGGT 1 cut(s) 755
Csp6I GTAC 4 cut(s) 196, 204, 224, 423
CspAI ACCGGT 1 cut(s) 364
CviAII CATG 2 cut(s) 415, 829
CviQI GTAC 4 cut(s) 196, 204, 224, 423
DdeI CTNAG 1 cut(s) 603
DpnI GATC 8 cut(s) 129, 165, 455, 599, 657, 822, 956, 1035
DpnII GATC 8 cut(s) 127, 163, 453, 597, 655, 820, 954, 1033
EaeI YGGCCR 1 cut(s) 929
Eam1104I CTCTTC 1 cut(s) 580
EarI CTCTTC 1 cut(s) 580
EciI GGCGGA 1 cut(s) 753
Eco130I CCWWGG 1 cut(s) 434
Eco47I GGWCC 1 cut(s) 395
Eco81I CCTNAGG 1 cut(s) 603
Eco88I CYCGRG 2 cut(s) 407, 504
EcoO109I RGGNCCY 1 cut(s) 395
EcoRII CCWGG 3 cut(s) 755, 796, 931
EcoT14I CCWWGG 1 cut(s) 434
ErhI CCWWGG 1 cut(s) 434
FaeI CATG 2 cut(s) 418, 832
FalI AAGNNNNNCTT 2 cut(s) 930, 962
FaqI GGGAC 1 cut(s) 574
FatI CATG 2 cut(s) 414, 828
FauNDI CATATG 1 cut(s) 851
FbaI TGATCA 2 cut(s) 127, 1033
FokI GGATG 2 cut(s) 536, 994
FspBI CTAG 1 cut(s) 734
HaeIII GGCC 2 cut(s) 750, 931
HapII CCGG 2 cut(s) 365, 659
HgaI GACGC 2 cut(s) 167, 990
Hin1II CATG 2 cut(s) 418, 832
HincII GTYRAC 1 cut(s) 1042
HindII GTYRAC 1 cut(s) 1042
HinfI GANTC 5 cut(s) 507, 611, 786, 842, 924
HpaII CCGG 2 cut(s) 365, 659
HphI GGTGA 2 cut(s) 818, 872
Hpy166II GTNNAC 2 cut(s) 280, 1042
Hpy188I TCNGA 7 cut(s) 46, 91, 113, 127, 688, 923, 1012
Hpy188III TCNNGA 4 cut(s) 232, 269, 457, 659
Hpy8I GTNNAC 2 cut(s) 280, 1042
Hpy99I CGWCG 1 cut(s) 161
HpyAV CCTTC 2 cut(s) 236, 454
HpyCH4III ACNGT 2 cut(s) 422, 860
HpyCH4IV ACGT 3 cut(s) 324, 893, 942
HpyCH4V TGCA 4 cut(s) 67, 443, 470, 883
HpyF10VI GCNNNNNNNGC 1 cut(s) 73
HpyF3I CTNAG 1 cut(s) 603
HpySE526I ACGT 3 cut(s) 324, 893, 942
Hsp92II CATG 2 cut(s) 418, 832
Kpn2I TCCGGA 1 cut(s) 658
Ksp22I TGATCA 2 cut(s) 127, 1033
Kzo9I GATC 8 cut(s) 127, 163, 453, 597, 655, 820, 954, 1033
LmnI GCTCC 2 cut(s) 57, 73
LweI GCATC 1 cut(s) 881
MabI ACCWGGT 1 cut(s) 755
MaeI CTAG 1 cut(s) 734
MaeII ACGT 3 cut(s) 324, 893, 942
MaeIII GTNAC 3 cut(s) 314, 429, 715
MalI GATC 8 cut(s) 129, 165, 455, 599, 657, 822, 956, 1035
MboI GATC 8 cut(s) 127, 163, 453, 597, 655, 820, 954, 1033
MboII GAAGA 9 cut(s) 224, 255, 479, 566, 567, 722, 821, 996, 998
MlsI TGGCCA 1 cut(s) 931
MluCI AATT 3 cut(s) 132, 812, 1029
MluNI TGGCCA 1 cut(s) 931
MlyI GAGTC 1 cut(s) 516
MnlI CCTC 6 cut(s) 64, 403, 408, 513, 543, 598
Mox20I TGGCCA 1 cut(s) 931
MroI TCCGGA 1 cut(s) 658
MroXI GAANNNNTTC 1 cut(s) 118
MscI TGGCCA 1 cut(s) 931
MseI TTAA 3 cut(s) 138, 449, 975
Msp20I TGGCCA 1 cut(s) 931
MspCI CTTAAG 1 cut(s) 974
MspI CCGG 2 cut(s) 365, 659
MspR9I CCNGG 3 cut(s) 757, 798, 933
MvaI CCWGG 3 cut(s) 757, 798, 933
MvnI CGCG 2 cut(s) 83, 285
MwoI GCNNNNNNNGC 1 cut(s) 73
NdeI CATATG 1 cut(s) 851
NdeII GATC 8 cut(s) 127, 163, 453, 597, 655, 820, 954, 1033
NlaIII CATG 2 cut(s) 418, 832
NmuCI GTSAC 1 cut(s) 314
PaeR7I CTCGAG 2 cut(s) 407, 504
PdmI GAANNNNTTC 1 cut(s) 118
PfeI GAWTC 4 cut(s) 611, 786, 842, 924
PflMI CCANNNNNTGG 1 cut(s) 797
PinAI ACCGGT 1 cut(s) 364
PleI GAGTC 1 cut(s) 515
PpsI GAGTC 1 cut(s) 515
PpuMI RGGWCCY 1 cut(s) 395
PsiI TTATAA 1 cut(s) 360
Psp1406I AACGTT 2 cut(s) 324, 893
Psp5II RGGWCCY 1 cut(s) 395
Psp6I CCWGG 3 cut(s) 755, 796, 931
PspGI CCWGG 3 cut(s) 755, 796, 931
PspPI GGNCC 1 cut(s) 395
PspPPI RGGWCCY 1 cut(s) 395
PspXI VCTCGAGB 2 cut(s) 407, 504
PsrI GAACNNNNNNTAC 2 cut(s) 207, 239
PstNI CAGNNNCTG 1 cut(s) 927
RsaI GTAC 4 cut(s) 197, 205, 225, 424
RsaNI GTAC 4 cut(s) 196, 204, 224, 423
SaqAI TTAA 3 cut(s) 138, 449, 975
Sau3AI GATC 8 cut(s) 127, 163, 453, 597, 655, 820, 954, 1033
Sau96I GGNCC 1 cut(s) 395
ScaI AGTACT 1 cut(s) 225
SchI GAGTC 1 cut(s) 516
ScrFI CCNGG 3 cut(s) 757, 798, 933
SexAI ACCWGGT 1 cut(s) 755
SfaNI GCATC 1 cut(s) 881
SfcI CTRYAG 2 cut(s) 207, 703
Sfr274I CTCGAG 2 cut(s) 407, 504
SinI GGWCC 1 cut(s) 395
SlaI CTCGAG 2 cut(s) 407, 504
SmlI CTYRAG 3 cut(s) 407, 504, 974
SmoI CTYRAG 3 cut(s) 407, 504, 974
Sse9I AATT 3 cut(s) 132, 812, 1029
SsiI CCGC 5 cut(s) 283, 293, 476, 738, 818
SspMI CTAG 1 cut(s) 734
StyD4I CCNGG 3 cut(s) 755, 796, 931
StyI CCWWGG 1 cut(s) 434
TaaI ACNGT 2 cut(s) 422, 860
TaiI ACGT 3 cut(s) 327, 896, 945
TaqI TCGA 6 cut(s) 156, 302, 408, 505, 596, 957
TasI AATT 3 cut(s) 132, 812, 1029
TatI WGTACW 1 cut(s) 223
TfiI GAWTC 4 cut(s) 611, 786, 842, 924
Tru1I TTAA 3 cut(s) 138, 449, 975
Tru9I TTAA 3 cut(s) 138, 449, 975
TseFI GTSAC 1 cut(s) 314
Tsp45I GTSAC 1 cut(s) 314
TspDTI ATGAA 3 cut(s) 17, 817, 996
TspGWI ACGGA 4 cut(s) 35, 232, 417, 640
Van91I CCANNNNNTGG 1 cut(s) 797
Vha464I CTTAAG 1 cut(s) 974
VpaK11BI GGWCC 1 cut(s) 395
XapI RAATTY 1 cut(s) 132
XhoI CTCGAG 2 cut(s) 407, 504
XmnI GAANNNNTTC 1 cut(s) 118
XspI CTAG 1 cut(s) 734
ZrmI AGTACT 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.