AT4G08545

leucine-rich repeat receptor-like serine threonine-protein kinase At3g14840

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
N/A
Physical Location & Seq
Forward (+)
5436600 .. 5439167
2568 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G08545.1

Sequence Viewer

Length: 426 bp
ATGGTAACATCGCCTAGAATCTCGGAGGCAAAATCTTATGACAGTGCATTGGCTTATGTGGAGAGTTGTGTTCATTACTCAAACCCTCAATCTGTGTGTCATCTATGGAAGCATAACAACTTGGCGCAATTTGTAGATCTGCGACTACAAAGCTATGACATGGATGAGGTAATGAAGACAATCAAAGTTGGGATCTTCTATACGAGTCGTGCAGCTTCTGATAGGCCACCAATGTTAGCAGTAGTTAGTATGTTAGAGGGTCTTCTAAGTGTAGAAGTCGAGATGCAAGCGCTTATGGCGGTTCTCAGTGATGAGGAGCAATCTCCGGATCTGTTGAGGATGATGGCGGACATTGACGAAGAATTCGAATTACAGGAGATTATAGAGACTCTCGAGAGGTATCCTGTCTCGACAAAGGGAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_8 Malectin Pkinase Pkinase_Tyr

Protein Analysis

141

Amino Acids

16.03

Weight (kDa)

4.58

Isoelectric Point (pI)

63.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 325
AciI CCGC 2 cut(s) 299, 347
AclWI GGATC 2 cut(s) 200, 336
AcsI RAATTY 1 cut(s) 362
AfeI AGCGCT 1 cut(s) 291
AluBI AGCT 2 cut(s) 153, 215
AluI AGCT 2 cut(s) 153, 215
Alw26I GTCTC 2 cut(s) 380, 412
AlwI GGATC 2 cut(s) 200, 336
AlwNI CAGNNNCTG 1 cut(s) 218
Ama87I CYCGRG 1 cut(s) 392
Aor13HI TCCGGA 1 cut(s) 325
Aor51HI AGCGCT 1 cut(s) 291
AoxI GGCC 1 cut(s) 224
ApeKI GCWGC 1 cut(s) 212
ApoI RAATTY 1 cut(s) 362
ArsI GACNNNNNNTTYG 2 cut(s) 347, 379
AspLEI GCGC 2 cut(s) 127, 292
AsuII TTCGAA 1 cut(s) 366
AvaI CYCGRG 1 cut(s) 392
BbsI GAAGAC 2 cut(s) 182, 254
BbvI GCAGC 1 cut(s) 224
BccI CCATC 1 cut(s) 337
BciVI GTATCC 1 cut(s) 411
BcoDI GTCTC 2 cut(s) 380, 412
BfaI CTAG 1 cut(s) 15
BfoI RGCGCY 1 cut(s) 293
BfuI GTATCC 1 cut(s) 411
BglII AGATCT 1 cut(s) 136
BisI GCNGC 1 cut(s) 213
BlsI GCNGC 1 cut(s) 214
BmeT110I CYCGRG 1 cut(s) 392
BmsI GCATC 1 cut(s) 273
BpiI GAAGAC 2 cut(s) 182, 254
Bpu14I TTCGAA 1 cut(s) 366
BsaWI WCCGGW 1 cut(s) 325
BseAI TCCGGA 1 cut(s) 325
BseGI GGATG 2 cut(s) 169, 345
BseMII CTCAG 1 cut(s) 319
BseRI GAGGAG 1 cut(s) 329
BseXI GCAGC 1 cut(s) 224
BsgI GTGCAG 1 cut(s) 231
BshFI GGCC 1 cut(s) 226
BsiHKCI CYCGRG 1 cut(s) 392
BsiSI CCGG 1 cut(s) 326
BsmAI GTCTC 2 cut(s) 380, 412
BsnI GGCC 1 cut(s) 226
BsoBI CYCGRG 1 cut(s) 392
Bsp119I TTCGAA 1 cut(s) 366
Bsp13I TCCGGA 1 cut(s) 325
Bsp143I GATC 3 cut(s) 136, 192, 328
BspACI CCGC 2 cut(s) 299, 347
BspANI GGCC 1 cut(s) 226
BspCNI CTCAG 1 cut(s) 318
BspEI TCCGGA 1 cut(s) 325
BspPI GGATC 2 cut(s) 200, 336
BspT104I TTCGAA 1 cut(s) 366
BssMI GATC 3 cut(s) 136, 192, 328
Bst4CI ACNGT 1 cut(s) 44
BstBI TTCGAA 1 cut(s) 366
BstC8I GCNNGC 1 cut(s) 288
BstDEI CTNAG 2 cut(s) 266, 305
BstF5I GGATG 2 cut(s) 169, 345
BstH2I RGCGCY 1 cut(s) 293
BstHHI GCGC 2 cut(s) 127, 292
BstKTI GATC 3 cut(s) 139, 195, 331
BstMAI GTCTC 2 cut(s) 380, 412
BstMBI GATC 3 cut(s) 136, 192, 328
BstMWI GCNNNNNNNGC 1 cut(s) 296
BstV1I GCAGC 1 cut(s) 224
BstV2I GAAGAC 2 cut(s) 182, 254
BstX2I RGATCY 3 cut(s) 136, 192, 328
BstYI RGATCY 3 cut(s) 136, 192, 328
BsuI GTATCC 1 cut(s) 411
BsuRI GGCC 1 cut(s) 226
BtsCI GGATG 2 cut(s) 169, 345
BtsIMutI CAGTG 2 cut(s) 49, 313
Cac8I GCNNGC 1 cut(s) 288
CaiI CAGNNNCTG 1 cut(s) 218
CfoI GCGC 2 cut(s) 127, 292
CviAII CATG 1 cut(s) 160
CviJI RGCY 4 cut(s) 53, 153, 215, 226
CviKI_1 RGCY 4 cut(s) 53, 153, 215, 226
DdeI CTNAG 2 cut(s) 266, 305
DpnI GATC 3 cut(s) 138, 194, 330
DpnII GATC 3 cut(s) 136, 192, 328
EciI GGCGGA 1 cut(s) 362
Eco47III AGCGCT 1 cut(s) 291
Eco88I CYCGRG 1 cut(s) 392
EcoRI GAATTC 1 cut(s) 362
FaeI CATG 1 cut(s) 163
FatI CATG 1 cut(s) 159
Fnu4HI GCNGC 1 cut(s) 213
FokI GGATG 2 cut(s) 176, 352
Fsp4HI GCNGC 1 cut(s) 213
FspBI CTAG 1 cut(s) 15
GlaI GCGC 2 cut(s) 126, 291
GluI GCNGC 1 cut(s) 213
HaeII RGCGCY 1 cut(s) 293
HaeIII GGCC 1 cut(s) 226
HapII CCGG 1 cut(s) 326
HhaI GCGC 2 cut(s) 127, 292
Hin1II CATG 1 cut(s) 163
Hin6I GCGC 2 cut(s) 125, 290
HinP1I GCGC 2 cut(s) 125, 290
HinfI GANTC 3 cut(s) 18, 205, 388
HpaII CCGG 1 cut(s) 326
Hpy188I TCNGA 2 cut(s) 25, 220
Hpy188III TCNNGA 5 cut(s) 280, 326, 392, 394, 409
HpyCH4III ACNGT 1 cut(s) 44
HpyCH4V TGCA 3 cut(s) 47, 212, 286
HpyF10VI GCNNNNNNNGC 1 cut(s) 296
HpyF3I CTNAG 2 cut(s) 266, 305
Hsp92II CATG 1 cut(s) 163
HspAI GCGC 2 cut(s) 125, 290
Kpn2I TCCGGA 1 cut(s) 325
Kzo9I GATC 3 cut(s) 136, 192, 328
LmnI GCTCC 1 cut(s) 316
LpnPI CCDG 3 cut(s) 339, 359, 417
Lsp1109I GCAGC 1 cut(s) 224
LweI GCATC 1 cut(s) 273
MaeI CTAG 1 cut(s) 15
MaeIII GTNAC 1 cut(s) 4
MalI GATC 3 cut(s) 138, 194, 330
MboI GATC 3 cut(s) 136, 192, 328
MboII GAAGA 4 cut(s) 187, 187, 254, 371
MflI RGATCY 3 cut(s) 136, 192, 328
MluCI AATT 3 cut(s) 128, 362, 368
MlyI GAGTC 2 cut(s) 214, 382
MnlI CCTC 7 cut(s) 19, 96, 160, 250, 307, 330, 390
MroI TCCGGA 1 cut(s) 325
MspI CCGG 1 cut(s) 326
MwoI GCNNNNNNNGC 1 cut(s) 296
NdeII GATC 3 cut(s) 136, 192, 328
NlaIII CATG 1 cut(s) 163
NspV TTCGAA 1 cut(s) 366
PaeR7I CTCGAG 1 cut(s) 392
PcsI WCGNNNNNNNCGW 1 cut(s) 363
PfeI GAWTC 1 cut(s) 18
PkrI GCNGC 1 cut(s) 214
PleI GAGTC 2 cut(s) 213, 382
PpsI GAGTC 2 cut(s) 213, 382
PstNI CAGNNNCTG 1 cut(s) 218
PsuI RGATCY 3 cut(s) 136, 192, 328
SatI GCNGC 1 cut(s) 213
Sau3AI GATC 3 cut(s) 136, 192, 328
SchI GAGTC 2 cut(s) 214, 382
SetI ASST 4 cut(s) 155, 171, 217, 401
SfaNI GCATC 1 cut(s) 273
Sfr274I CTCGAG 1 cut(s) 392
SfuI TTCGAA 1 cut(s) 366
SlaI CTCGAG 1 cut(s) 392
SmlI CTYRAG 1 cut(s) 392
SmoI CTYRAG 1 cut(s) 392
Sse9I AATT 3 cut(s) 128, 362, 368
SsiI CCGC 2 cut(s) 299, 347
SspMI CTAG 1 cut(s) 15
TaaI ACNGT 1 cut(s) 44
TaqI TCGA 4 cut(s) 279, 366, 393, 410
TasI AATT 3 cut(s) 128, 362, 368
TfiI GAWTC 1 cut(s) 18
TscAI CASTG 2 cut(s) 49, 313
TseI GCWGC 1 cut(s) 212
TspDTI ATGAA 2 cut(s) 62, 188
TspRI CASTG 2 cut(s) 49, 313
XapI RAATTY 1 cut(s) 362
XhoI CTCGAG 1 cut(s) 392
XspI CTAG 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.