AT4G12350
MYB Family

myb domain protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
N/A
Physical Location & Seq
Forward (+)
7324756 .. 7326867
2112 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G12350.1

Sequence Viewer

Length: 861 bp
ATGGGAAGGCAGCCATGTTGTGACAAGCTCATGGTGAAGAAGGGGCCGTGGACGGCGGAGGAAGACAAGAAACTGATAAACTTTATCTTGACCAACGGCCACTGTTGCTGGAGGGCTTTGCCGAAGCTGGCCGGTCTCCGTCGCTGTGGGAAGAGCTGCCGTCTACGGTGGACCAATTATCTCCGACCTGACTTGAAGAGAGGTCTTCTCTCCGACGCCGAGGAACAGCTTGTCATCGACCTTCATGCTCTTCTCGGCAACAGATGGTCCAAGATCGCTGCAAGATTACCAGGAAGAACAGACAACGAAATAAAAAATCATTGGAATACTCATATCAAGAAGAAGCTCCTTAAGATGGAAATCGATCCTTCGACCCATCAACCTTTAAACAAAGTATTTACCGATACAAACTTAGTCGATAAATCTGAAACTTCATCGAAAGCCGACAATGTAAATGATAATAAAATCGTAGAGATCGATGGGACAACGACAAATACAATAGATGATAGCATTATCACTCATCAAAATAGTTCAAATGATGATTATGAATTACTTGGTGATATAATTCATAATTATGGAGATTTATTTAATATTCTATGGACCAACGATGAACCTCCTCTAGTCGATGATGCATCATGGAGCAATCATAACGTTGGTATTGGAGGAACAGCTGCAGTTGCAGCCTCAGACAAGAACAACACTGCTGCCGAGGAAGATTTCCCGGAAAGATCATTTGAAAAACAGAACGGCGAAAGTTGGATGTTCTTGGATTATTGCCAAGAATTTGGTGTTGAAGATTTTGGGTTCGAGTGTTACCATGGTTTTGGTCAAAGCTCCATGAAGACGGGTCACAAGGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

286

Amino Acids

32.4

Weight (kDa)

5.49

Isoelectric Point (pI)

41.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 14 - 61 1e-14 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 17 - 76 1.1e-08 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 71 - 111 7e-16 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 71 - 123 1.9e-07 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 163
AciI CCGC 1 cut(s) 56
AclI AACGTT 1 cut(s) 651
AclWI GGATC 1 cut(s) 359
AcoI YGGCCR 2 cut(s) 97, 129
AcsI RAATTY 1 cut(s) 782
AcyI GRCGYC 1 cut(s) 216
AfiI CCNNNNNNNGG 1 cut(s) 355
AflII CTTAAG 1 cut(s) 350
AgsI TTSAA 4 cut(s) 196, 534, 737, 794
AjnI CCWGG 1 cut(s) 289
AluBI AGCT 7 cut(s) 28, 127, 156, 229, 346, 671, 834
AluI AGCT 7 cut(s) 28, 127, 156, 229, 346, 671, 834
Alw26I GTCTC 1 cut(s) 140
AlwI GGATC 1 cut(s) 359
AoxI GGCC 3 cut(s) 44, 97, 129
ApeKI GCWGC 6 cut(s) 10, 156, 278, 671, 680, 704
ApoI RAATTY 1 cut(s) 782
AspS9I GGNCC 4 cut(s) 44, 171, 267, 600
AsuC2I CCSGG 1 cut(s) 722
AsuHPI GGTGA 2 cut(s) 46, 569
AvaII GGWCC 3 cut(s) 171, 267, 600
BbsI GAAGAC 3 cut(s) 69, 197, 848
BbvI GCAGC 6 cut(s) 22, 143, 265, 658, 691, 692
BccI CCATC 4 cut(s) 258, 349, 384, 473
BceAI ACGGC 5 cut(s) 31, 69, 112, 144, 763
BcgI CGANNNNNNTGC 2 cut(s) 227, 261
BciT130I CCWGG 1 cut(s) 291
BcnI CCSGG 1 cut(s) 722
BcoDI GTCTC 1 cut(s) 140
BfaI CTAG 2 cut(s) 620, 859
BfmI CTRYAG 1 cut(s) 672
BfrI CTTAAG 1 cut(s) 350
BisI GCNGC 6 cut(s) 11, 157, 279, 672, 681, 705
BlsI GCNGC 6 cut(s) 12, 158, 280, 673, 682, 706
Bme1390I CCNGG 2 cut(s) 291, 722
Bme18I GGWCC 3 cut(s) 171, 267, 600
BmgT120I GGNCC 4 cut(s) 44, 171, 267, 600
BmiI GGNNCC 1 cut(s) 45
BmrFI CCNGG 2 cut(s) 291, 722
BmsI GCATC 2 cut(s) 619, 641
BpiI GAAGAC 3 cut(s) 69, 197, 848
BplI GAGNNNNNCTC 2 cut(s) 192, 224
BpmI CTGGAG 1 cut(s) 130
BpuMI CCSGG 1 cut(s) 722
Bsa29I ATCGAT 2 cut(s) 363, 477
BsaBI GATNNNNATC 1 cut(s) 359
BsaHI GRCGYC 1 cut(s) 216
BsaI GGTCTC 1 cut(s) 140
BsaJI CCNNGG 4 cut(s) 47, 219, 708, 817
Bsc4I CCNNNNNNNGG 1 cut(s) 355
Bse118I RCCGGY 1 cut(s) 131
Bse8I GATNNNNATC 1 cut(s) 359
BseBI CCWGG 1 cut(s) 291
BseCI ATCGAT 2 cut(s) 363, 477
BseDI CCNNGG 4 cut(s) 47, 219, 708, 817
BseGI GGATG 1 cut(s) 765
BseJI GATNNNNATC 1 cut(s) 359
BseLI CCNNNNNNNGG 1 cut(s) 355
BseMII CTCAG 1 cut(s) 699
BseRI GAGGAG 1 cut(s) 606
BseXI GCAGC 6 cut(s) 22, 143, 265, 658, 691, 692
BshFI GGCC 3 cut(s) 46, 99, 131
BshVI ATCGAT 2 cut(s) 363, 477
BsiSI CCGG 2 cut(s) 132, 722
BslFI GGGAC 1 cut(s) 496
BslI CCNNNNNNNGG 1 cut(s) 355
BsmAI GTCTC 1 cut(s) 140
BsmFI GGGAC 1 cut(s) 496
BsnI GGCC 3 cut(s) 46, 99, 131
Bso31I GGTCTC 1 cut(s) 140
Bsp143I GATC 4 cut(s) 273, 364, 474, 728
Bsp19I CCATGG 1 cut(s) 817
BspACI CCGC 1 cut(s) 56
BspANI GGCC 3 cut(s) 46, 99, 131
BspCNI CTCAG 1 cut(s) 698
BspDI ATCGAT 2 cut(s) 363, 477
BspLI GGNNCC 1 cut(s) 45
BspMAI CTGCAG 1 cut(s) 676
BspPI GGATC 1 cut(s) 359
BspQI GCTCTTC 2 cut(s) 146, 255
BspTI CTTAAG 1 cut(s) 350
BspTNI GGTCTC 1 cut(s) 140
BsrFI RCCGGY 1 cut(s) 131
BssAI RCCGGY 1 cut(s) 131
BssECI CCNNGG 4 cut(s) 47, 219, 708, 817
BssMI GATC 4 cut(s) 273, 364, 474, 728
BssNI GRCGYC 1 cut(s) 216
BssT1I CCWWGG 1 cut(s) 817
Bst2UI CCWGG 1 cut(s) 291
Bst4CI ACNGT 2 cut(s) 104, 168
Bst6I CTCTTC 3 cut(s) 146, 191, 255
BstACI GRCGYC 1 cut(s) 216
BstAFI CTTAAG 1 cut(s) 350
BstC8I GCNNGC 1 cut(s) 129
BstDEI CTNAG 2 cut(s) 412, 685
BstDSI CCRYGG 2 cut(s) 47, 817
BstF5I GGATG 1 cut(s) 765
BstKTI GATC 4 cut(s) 276, 367, 477, 731
BstMAI GTCTC 1 cut(s) 140
BstMBI GATC 4 cut(s) 273, 364, 474, 728
BstMWI GCNNNNNNNGC 3 cut(s) 105, 677, 680
BstNI CCWGG 1 cut(s) 291
BstSCI CCNGG 2 cut(s) 289, 720
BstSFI CTRYAG 1 cut(s) 672
BstV1I GCAGC 6 cut(s) 22, 143, 265, 658, 691, 692
BstV2I GAAGAC 3 cut(s) 69, 197, 848
BstXI CCANNNNNNTGG 2 cut(s) 785, 824
Bsu15I ATCGAT 2 cut(s) 363, 477
BsuRI GGCC 3 cut(s) 46, 99, 131
BsuTUI ATCGAT 2 cut(s) 363, 477
BtgI CCRYGG 2 cut(s) 47, 817
BtsCI GGATG 1 cut(s) 765
BtsI GCAGTG 1 cut(s) 699
BtsIMutI CAGTG 2 cut(s) 100, 699
Cac8I GCNNGC 1 cut(s) 129
Cfr10I RCCGGY 1 cut(s) 131
Cfr13I GGNCC 4 cut(s) 44, 171, 267, 600
ClaI ATCGAT 2 cut(s) 363, 477
CseI GACGC 1 cut(s) 224
CviAII CATG 6 cut(s) 15, 31, 245, 636, 818, 838
DdeI CTNAG 2 cut(s) 412, 685
DpnI GATC 4 cut(s) 275, 366, 476, 730
DpnII GATC 4 cut(s) 273, 364, 474, 728
DraI TTTAAA 1 cut(s) 387
EaeI YGGCCR 2 cut(s) 97, 129
Eam1104I CTCTTC 3 cut(s) 146, 191, 255
EarI CTCTTC 3 cut(s) 146, 191, 255
EciI GGCGGA 1 cut(s) 71
Eco130I CCWWGG 1 cut(s) 817
Eco31I GGTCTC 1 cut(s) 140
Eco47I GGWCC 3 cut(s) 171, 267, 600
EcoRII CCWGG 1 cut(s) 289
EcoT14I CCWWGG 1 cut(s) 817
EcoT22I ATGCAT 1 cut(s) 634
ErhI CCWWGG 1 cut(s) 817
FaeI CATG 6 cut(s) 18, 34, 248, 639, 821, 841
FaqI GGGAC 1 cut(s) 496
FatI CATG 6 cut(s) 14, 30, 244, 635, 817, 837
FblI GTMKAC 1 cut(s) 163
Fnu4HI GCNGC 6 cut(s) 11, 157, 279, 672, 681, 705
FokI GGATG 1 cut(s) 772
Fsp4HI GCNGC 6 cut(s) 11, 157, 279, 672, 681, 705
FspBI CTAG 2 cut(s) 620, 859
GluI GCNGC 6 cut(s) 11, 157, 279, 672, 681, 705
GsuI CTGGAG 1 cut(s) 130
HaeIII GGCC 3 cut(s) 46, 99, 131
HapII CCGG 2 cut(s) 132, 722
HgaI GACGC 1 cut(s) 224
Hin1I GRCGYC 1 cut(s) 216
Hin1II CATG 6 cut(s) 18, 34, 248, 639, 821, 841
HpaII CCGG 2 cut(s) 132, 722
HphI GGTGA 2 cut(s) 46, 569
Hpy166II GTNNAC 3 cut(s) 51, 164, 171
Hpy188I TCNGA 4 cut(s) 185, 214, 427, 688
Hpy188III TCNNGA 2 cut(s) 88, 337
Hpy8I GTNNAC 3 cut(s) 51, 164, 171
Hpy99I CGWCG 2 cut(s) 144, 218
HpyAV CCTTC 3 cut(s) 34, 251, 378
HpyCH4III ACNGT 2 cut(s) 104, 168
HpyCH4IV ACGT 1 cut(s) 651
HpyCH4V TGCA 4 cut(s) 281, 632, 674, 680
HpyF10VI GCNNNNNNNGC 3 cut(s) 105, 677, 680
HpyF3I CTNAG 2 cut(s) 412, 685
HpySE526I ACGT 1 cut(s) 651
Hsp92I GRCGYC 1 cut(s) 216
Hsp92II CATG 6 cut(s) 18, 34, 248, 639, 821, 841
Kzo9I GATC 4 cut(s) 273, 364, 474, 728
LguI GCTCTTC 2 cut(s) 146, 255
LmnI GCTCC 3 cut(s) 351, 639, 839
LpnPI CCDG 7 cut(s) 94, 113, 145, 201, 276, 303, 735
Lsp1109I GCAGC 6 cut(s) 22, 143, 265, 658, 691, 692
LweI GCATC 2 cut(s) 619, 641
MaeI CTAG 2 cut(s) 620, 859
MaeII ACGT 1 cut(s) 651
MaeIII GTNAC 3 cut(s) 20, 812, 848
MalI GATC 4 cut(s) 275, 366, 476, 730
MboI GATC 4 cut(s) 273, 364, 474, 728
MluCI AATT 5 cut(s) 175, 548, 564, 571, 782
MmeI TCCRAC 3 cut(s) 208, 237, 737
MnlI CCTC 9 cut(s) 52, 105, 194, 214, 624, 627, 656, 694, 703
Mph1103I ATGCAT 1 cut(s) 634
MseI TTAA 3 cut(s) 351, 386, 588
MslI CAYNNNNRTG 1 cut(s) 573
MspA1I CMGCKG 1 cut(s) 671
MspCI CTTAAG 1 cut(s) 350
MspI CCGG 2 cut(s) 132, 722
MspR9I CCNGG 2 cut(s) 291, 722
MvaI CCWGG 1 cut(s) 291
MwoI GCNNNNNNNGC 3 cut(s) 105, 677, 680
NciI CCSGG 1 cut(s) 722
NcoI CCATGG 1 cut(s) 817
NdeII GATC 4 cut(s) 273, 364, 474, 728
NlaIII CATG 6 cut(s) 18, 34, 248, 639, 821, 841
NlaIV GGNNCC 1 cut(s) 45
NmeAIII GCCGAG 3 cut(s) 234, 244, 733
NmuCI GTSAC 2 cut(s) 20, 848
NsiI ATGCAT 1 cut(s) 634
PciSI GCTCTTC 2 cut(s) 146, 255
PcsI WCGNNNNNNNCGW 1 cut(s) 474
PfoI TCCNGGA 1 cut(s) 720
PkrI GCNGC 6 cut(s) 12, 158, 280, 673, 682, 706
Psp1406I AACGTT 1 cut(s) 651
Psp6I CCWGG 1 cut(s) 289
PspGI CCWGG 1 cut(s) 289
PspN4I GGNNCC 1 cut(s) 45
PspPI GGNCC 4 cut(s) 44, 171, 267, 600
PstI CTGCAG 1 cut(s) 676
PvuII CAGCTG 1 cut(s) 671
RseI CAYNNNNRTG 1 cut(s) 573
SapI GCTCTTC 2 cut(s) 146, 255
SaqAI TTAA 3 cut(s) 351, 386, 588
SatI GCNGC 6 cut(s) 11, 157, 279, 672, 681, 705
Sau3AI GATC 4 cut(s) 273, 364, 474, 728
Sau96I GGNCC 4 cut(s) 44, 171, 267, 600
ScrFI CCNGG 2 cut(s) 291, 722
SfaNI GCATC 2 cut(s) 619, 641
SfcI CTRYAG 1 cut(s) 672
SinI GGWCC 3 cut(s) 171, 267, 600
SmiMI CAYNNNNRTG 1 cut(s) 573
SmlI CTYRAG 1 cut(s) 350
SmoI CTYRAG 1 cut(s) 350
Sse9I AATT 5 cut(s) 175, 548, 564, 571, 782
SsiI CCGC 1 cut(s) 56
SspI AATATT 1 cut(s) 592
SspMI CTAG 2 cut(s) 620, 859
StyD4I CCNGG 2 cut(s) 289, 720
StyI CCWWGG 1 cut(s) 817
TaaI ACNGT 2 cut(s) 104, 168
TaiI ACGT 1 cut(s) 654
TaqI TCGA 8 cut(s) 237, 363, 371, 417, 437, 477, 624, 807
TasI AATT 5 cut(s) 175, 548, 564, 571, 782
Tru1I TTAA 3 cut(s) 351, 386, 588
Tru9I TTAA 3 cut(s) 351, 386, 588
TscAI CASTG 2 cut(s) 107, 706
TseFI GTSAC 2 cut(s) 20, 848
TseI GCWGC 6 cut(s) 10, 156, 278, 671, 680, 704
Tsp45I GTSAC 2 cut(s) 20, 848
TspDTI ATGAA 6 cut(s) 233, 423, 557, 561, 624, 854
TspGWI ACGGA 1 cut(s) 128
TspRI CASTG 2 cut(s) 107, 706
Vha464I CTTAAG 1 cut(s) 350
VpaK11BI GGWCC 3 cut(s) 171, 267, 600
XapI RAATTY 1 cut(s) 782
XmiI GTMKAC 1 cut(s) 163
XspI CTAG 2 cut(s) 620, 859
Zsp2I ATGCAT 1 cut(s) 634
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.