AT4G29654

receptor protein kinase TMK1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
N/A
Physical Location & Seq
Reverse (-)
14531601 .. 14532454
854 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G29654.1

Sequence Viewer

Length: 477 bp
ATGCATTTTAAAATAGTTGGTTTGAGGATATTTTGGTTTGACCCGATCTACAGCAGAGGGCAACGAATCAATCATGACAAAGTCTATCCTCGGCACATATGCTTAAACTTTGTAAGTGCATTGATTGGAAGGGTAACTACAAAAGCTGATGTCTACAGTTTTGGAGTTATTCTCATGGAGTTAGTCACAAGCAAGGAAGCTTTAGACAAGAAGCGATCAGATGCTGAACATCACATTCTCAAGAACCGCAACATCATTGACGACGTAGCGAGACTAGCAATTCACTGCTGCGCCAAGGAGCTCACTCAGAGACCAGAGATGAAGTATGTTGTCTCTACACTTACCTCACTCTGTGACACTTGGAAGCCTAGTGAAATCGTAGACAACGACAGGGAGGACACAAGTGTATTAGAACTTGTAAAGAGATGGAAGGAGCAAGAGATGGAGGGAAGCAGCAGCGGTATCATTGTGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

158

Amino Acids

18.25

Weight (kDa)

7.71

Isoelectric Point (pI)

38.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 44 - 114 2.4e-07 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 153, 381
AciI CCGC 2 cut(s) 247, 459
AdeI CACNNNGTG 1 cut(s) 353
AluBI AGCT 3 cut(s) 146, 200, 301
AluI AGCT 3 cut(s) 146, 200, 301
Alw21I GWGCWC 1 cut(s) 303
Alw26I GTCTC 3 cut(s) 265, 304, 337
AlwNI CAGNNNCTG 1 cut(s) 224
ApeKI GCWGC 3 cut(s) 288, 453, 456
AspLEI GCGC 1 cut(s) 293
BanII GRGCYC 1 cut(s) 303
BarI GAAGNNNNNNTAC 2 cut(s) 121, 153
Bbv12I GWGCWC 1 cut(s) 303
BbvI GCAGC 3 cut(s) 275, 465, 468
BccI CCATC 2 cut(s) 420, 436
BcoDI GTCTC 3 cut(s) 265, 304, 337
BfaI CTAG 2 cut(s) 275, 369
BfmI CTRYAG 2 cut(s) 49, 154
BisI GCNGC 3 cut(s) 289, 454, 457
BlsI GCNGC 3 cut(s) 290, 455, 458
BmsI GCATC 1 cut(s) 211
BplI GAGNNNNNCTC 2 cut(s) 156, 188
BpuEI CTTGAG 1 cut(s) 224
BsaI GGTCTC 1 cut(s) 304
BsaJI CCNNGG 2 cut(s) 89, 294
BseDI CCNNGG 2 cut(s) 89, 294
BseMII CTCAG 1 cut(s) 320
BseXI GCAGC 3 cut(s) 275, 465, 468
BsiHKAI GWGCWC 1 cut(s) 303
BsmAI GTCTC 3 cut(s) 265, 304, 337
Bso31I GGTCTC 1 cut(s) 304
Bsp1286I GDGCHC 1 cut(s) 303
Bsp143I GATC 2 cut(s) 45, 215
BspACI CCGC 2 cut(s) 247, 459
BspCNI CTCAG 1 cut(s) 319
BspHI TCATGA 1 cut(s) 73
BspTNI GGTCTC 1 cut(s) 304
BssECI CCNNGG 2 cut(s) 89, 294
BssMI GATC 2 cut(s) 45, 215
BssT1I CCWWGG 1 cut(s) 294
Bst4CI ACNGT 1 cut(s) 158
BstDEI CTNAG 1 cut(s) 306
BstHHI GCGC 1 cut(s) 293
BstKTI GATC 2 cut(s) 48, 218
BstMAI GTCTC 3 cut(s) 265, 304, 337
BstMBI GATC 2 cut(s) 45, 215
BstMWI GCNNNNNNNGC 1 cut(s) 275
BstSFI CTRYAG 2 cut(s) 49, 154
BstV1I GCAGC 3 cut(s) 275, 465, 468
BtsI GCAGTG 1 cut(s) 283
BtsIMutI CAGTG 1 cut(s) 283
CaiI CAGNNNCTG 1 cut(s) 224
CciI TCATGA 1 cut(s) 73
CfoI GCGC 1 cut(s) 293
CviAII CATG 2 cut(s) 74, 175
CviJI RGCY 4 cut(s) 146, 200, 301, 367
CviKI_1 RGCY 4 cut(s) 146, 200, 301, 367
DdeI CTNAG 1 cut(s) 306
DpnI GATC 2 cut(s) 47, 217
DpnII GATC 2 cut(s) 45, 215
DraI TTTAAA 1 cut(s) 10
DraIII CACNNNGTG 1 cut(s) 353
Ecl136II GAGCTC 1 cut(s) 301
Eco130I CCWWGG 1 cut(s) 294
Eco24I GRGCYC 1 cut(s) 303
Eco31I GGTCTC 1 cut(s) 304
Eco53kI GAGCTC 1 cut(s) 301
EcoICRI GAGCTC 1 cut(s) 301
EcoT14I CCWWGG 1 cut(s) 294
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 303
ErhI CCWWGG 1 cut(s) 294
FaeI CATG 2 cut(s) 77, 178
FaiI YATR 5 cut(s) 75, 98, 100, 176, 327
FatI CATG 2 cut(s) 73, 174
FauNDI CATATG 1 cut(s) 98
FblI GTMKAC 2 cut(s) 153, 381
Fnu4HI GCNGC 3 cut(s) 289, 454, 457
FriOI GRGCYC 1 cut(s) 303
Fsp4HI GCNGC 3 cut(s) 289, 454, 457
FspBI CTAG 2 cut(s) 275, 369
GlaI GCGC 1 cut(s) 292
GluI GCNGC 3 cut(s) 289, 454, 457
HhaI GCGC 1 cut(s) 293
Hin1II CATG 2 cut(s) 77, 178
Hin6I GCGC 1 cut(s) 291
HinP1I GCGC 1 cut(s) 291
HindIII AAGCTT 1 cut(s) 198
HinfI GANTC 1 cut(s) 66
Hpy166II GTNNAC 2 cut(s) 154, 382
Hpy188I TCNGA 2 cut(s) 220, 309
Hpy188III TCNNGA 2 cut(s) 74, 241
Hpy8I GTNNAC 2 cut(s) 154, 382
Hpy99I CGWCG 1 cut(s) 266
HpyAV CCTTC 2 cut(s) 123, 424
HpyCH4III ACNGT 1 cut(s) 158
HpyCH4IV ACGT 1 cut(s) 264
HpyCH4V TGCA 2 cut(s) 4, 119
HpyF10VI GCNNNNNNNGC 1 cut(s) 275
HpyF3I CTNAG 1 cut(s) 306
HpySE526I ACGT 1 cut(s) 264
Hsp92II CATG 2 cut(s) 77, 178
HspAI GCGC 1 cut(s) 291
Kzo9I GATC 2 cut(s) 45, 215
LmnI GCTCC 2 cut(s) 298, 433
LpnPI CCDG 2 cut(s) 327, 376
Lsp1109I GCAGC 3 cut(s) 275, 465, 468
LweI GCATC 1 cut(s) 211
MaeI CTAG 2 cut(s) 275, 369
MaeII ACGT 1 cut(s) 264
MaeIII GTNAC 3 cut(s) 133, 184, 353
MalI GATC 2 cut(s) 47, 217
MboI GATC 2 cut(s) 45, 215
MhlI GDGCHC 1 cut(s) 303
MluCI AATT 1 cut(s) 279
MnlI CCTC 6 cut(s) 18, 50, 99, 355, 388, 439
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 2 cut(s) 9, 104
MspA1I CMGCKG 1 cut(s) 459
MwoI GCNNNNNNNGC 1 cut(s) 275
NdeI CATATG 1 cut(s) 98
NdeII GATC 2 cut(s) 45, 215
NlaIII CATG 2 cut(s) 77, 178
NmeAIII GCCGAG 1 cut(s) 70
NmuCI GTSAC 2 cut(s) 184, 353
NsiI ATGCAT 1 cut(s) 6
PagI TCATGA 1 cut(s) 73
PcsI WCGNNNNNNNCGW 1 cut(s) 384
PfeI GAWTC 1 cut(s) 66
PflFI GACNNNGTC 1 cut(s) 80
PkrI GCNGC 3 cut(s) 290, 455, 458
Psp124BI GAGCTC 1 cut(s) 303
PstNI CAGNNNCTG 1 cut(s) 224
PsyI GACNNNGTC 1 cut(s) 80
SacI GAGCTC 1 cut(s) 303
SaqAI TTAA 2 cut(s) 9, 104
SatI GCNGC 3 cut(s) 289, 454, 457
Sau3AI GATC 2 cut(s) 45, 215
SduI GDGCHC 1 cut(s) 303
SetI ASST 5 cut(s) 148, 202, 267, 303, 347
SfaNI GCATC 1 cut(s) 211
SfcI CTRYAG 2 cut(s) 49, 154
SmlI CTYRAG 1 cut(s) 239
SmoI CTYRAG 1 cut(s) 239
Sse9I AATT 1 cut(s) 279
SsiI CCGC 2 cut(s) 247, 459
SspMI CTAG 2 cut(s) 275, 369
SstI GAGCTC 1 cut(s) 303
StyI CCWWGG 1 cut(s) 294
TaaI ACNGT 1 cut(s) 158
TaiI ACGT 1 cut(s) 267
TasI AATT 1 cut(s) 279
TfiI GAWTC 1 cut(s) 66
Tru1I TTAA 2 cut(s) 9, 104
Tru9I TTAA 2 cut(s) 9, 104
TscAI CASTG 1 cut(s) 290
TseFI GTSAC 2 cut(s) 184, 353
TseI GCWGC 3 cut(s) 288, 453, 456
Tsp45I GTSAC 2 cut(s) 184, 353
TspDTI ATGAA 1 cut(s) 335
TspRI CASTG 1 cut(s) 290
Tth111I GACNNNGTC 1 cut(s) 80
XmiI GTMKAC 2 cut(s) 153, 381
XspI CTAG 2 cut(s) 275, 369
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.