FvH4_1g22970

wall-associated receptor kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
N/A
Physical Location & Seq
Reverse (-)
14866169 .. 14867604
1436 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g22970.t1

Sequence Viewer

Length: 879 bp
ATGTACTTTAACGCCCGTAACAATGGCTACTCGATGTTTCTGCTCAGACGGCTATCAGGGAAATCCGTATATTCTTCAGGGATGTCAAGTTGTGTGCTGCACCCTTGGAATATCCTTTCTAATCTTAGTGGCACGGTGGTTGAAAAGGTTCATCAAGAAATGGAAACAGAGAAAGAGCAGGGACAACAATTATCTACCGGCAAAGACCATGTTGTGAAAATTAAGTTGTCACCTCCAAGGAGTTGCTCAAAGCCACCTGCGAGTTTAATTCAAGGTACTGTGTACAAAGGAATGTTGGAAGATGGAATATTTTTTGCTGTCAAGAAGTCCAAAAGAGTTGATGCAGGCGACCGCGAAGTGGAACAGTTCATCAATGAAATTGTCATTCTTTCGCAAATCAACCACAGAAATGTCGTCAAGCAATTAGGTTGTTGCTTAGAGACAGAAGTTCCTCTTTTGGTTTATGAATTCATACTCAATGGAAGCCTTTCTCAATACATTCATCATCACAATGACTTTCATCTGACATGGGAAATGCGCTTACAAATTTTTATGCAAGTTGCCGGAGCTCTTTCCTACTTGCATGCATCTACTTGCTTGCCAATTTACCATCGAGACACCAAGTCTGATCCATATATTACCATTGACAAAACGCACCTTACAACACTAGTGAAAGGAGCATTTGGTTATATAGATCCAGGATATTTTCAATCCAGCCAATTTACGGACAAGAGTGACGTCTATAGCTTTGGAGTGGTCCTTGTTGAGCTCCTGACTGGACAAAAACCAGTTTTCTTGATACAATCTGATGAAGAATGGAAAAGCCTAGCAACTCATTTCATTGAGTCCATGGAAGAAAATAGTGTTTGGCATTATTGA

Protein Analysis

293

Amino Acids

33.28

Weight (kDa)

6.35

Isoelectric Point (pI)

39.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 92 - 206 8.1e-19 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 92 - 210 6.8e-17 Protein kinase domain
Pkinase PF00069 221 - 278 9e-06 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 228 - 273 1.3e-06 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 265
AatII GACGTC 1 cut(s) 741
Acc36I ACCTGC 1 cut(s) 265
AccII CGCG 1 cut(s) 354
AciI CCGC 1 cut(s) 352
AclWI GGATC 2 cut(s) 623, 689
AcsI RAATTY 2 cut(s) 467, 546
AcuI CTGAAG 1 cut(s) 60
AcyI GRCGYC 1 cut(s) 738
AfaI GTAC 3 cut(s) 5, 277, 284
AfiI CCNNNNNNNGG 2 cut(s) 358, 724
AgsI TTSAA 3 cut(s) 143, 272, 710
AhdI GACNNNNNGTC 1 cut(s) 622
AhlI ACTAGT 1 cut(s) 667
AjnI CCWGG 1 cut(s) 697
AluBI AGCT 3 cut(s) 569, 747, 769
AluI AGCT 3 cut(s) 569, 747, 769
Alw21I GWGCWC 2 cut(s) 571, 771
Alw26I GTCTC 2 cut(s) 434, 609
AlwI GGATC 2 cut(s) 623, 689
ApeKI GCWGC 1 cut(s) 97
ApoI RAATTY 2 cut(s) 467, 546
Asp700I GAANNNNTTC 2 cut(s) 147, 487
AspLEI GCGC 1 cut(s) 540
AspS9I GGNCC 1 cut(s) 757
AsuHPI GGTGA 1 cut(s) 222
AvaII GGWCC 1 cut(s) 757
BanII GRGCYC 2 cut(s) 571, 771
Bbv12I GWGCWC 2 cut(s) 571, 771
BbvI GCAGC 1 cut(s) 84
BccI CCATC 2 cut(s) 296, 618
BceAI ACGGC 1 cut(s) 65
BcgI CGANNNNNNTGC 2 cut(s) 22, 56
BciT130I CCWGG 1 cut(s) 699
BcoDI GTCTC 2 cut(s) 434, 609
BcuI ACTAGT 1 cut(s) 667
BfaI CTAG 2 cut(s) 668, 827
BfmI CTRYAG 1 cut(s) 742
BfuAI ACCTGC 1 cut(s) 265
BisI GCNGC 1 cut(s) 98
BlsI GCNGC 1 cut(s) 99
Bme1390I CCNGG 1 cut(s) 699
Bme18I GGWCC 1 cut(s) 757
BmeRI GACNNNNNGTC 1 cut(s) 622
BmgT120I GGNCC 1 cut(s) 757
BmrFI CCNGG 1 cut(s) 699
BmsI GCATC 2 cut(s) 331, 596
BsaHI GRCGYC 1 cut(s) 738
BsaJI CCNNGG 3 cut(s) 104, 236, 849
Bsc4I CCNNNNNNNGG 2 cut(s) 358, 724
Bse118I RCCGGY 1 cut(s) 197
Bse1I ACTGG 2 cut(s) 781, 788
BseBI CCWGG 1 cut(s) 699
BseDI CCNNGG 3 cut(s) 104, 236, 849
BseGI GGATG 1 cut(s) 87
BseLI CCNNNNNNNGG 2 cut(s) 358, 724
BseMII CTCAG 1 cut(s) 58
BseNI ACTGG 2 cut(s) 781, 788
BseXI GCAGC 1 cut(s) 84
BsgI GTGCAG 1 cut(s) 83
Bsh1236I CGCG 1 cut(s) 354
Bsh1285I CGRYCG 1 cut(s) 352
BsiEI CGRYCG 1 cut(s) 352
BsiHKAI GWGCWC 2 cut(s) 571, 771
BsiSI CCGG 2 cut(s) 198, 564
BslFI GGGAC 1 cut(s) 195
BslI CCNNNNNNNGG 2 cut(s) 358, 724
BsmAI GTCTC 2 cut(s) 434, 609
BsmFI GGGAC 1 cut(s) 195
Bsp1286I GDGCHC 2 cut(s) 571, 771
Bsp1407I TGTACA 1 cut(s) 282
Bsp143I GATC 2 cut(s) 628, 694
Bsp19I CCATGG 1 cut(s) 849
BspACI CCGC 1 cut(s) 352
BspCNI CTCAG 1 cut(s) 57
BspFNI CGCG 1 cut(s) 354
BspMI ACCTGC 1 cut(s) 265
BspPI GGATC 2 cut(s) 623, 689
BsrFI RCCGGY 1 cut(s) 197
BsrGI TGTACA 1 cut(s) 282
BsrI ACTGG 2 cut(s) 781, 788
BssAI RCCGGY 1 cut(s) 197
BssECI CCNNGG 3 cut(s) 104, 236, 849
BssMI GATC 2 cut(s) 628, 694
BssNI GRCGYC 1 cut(s) 738
BssT1I CCWWGG 3 cut(s) 104, 236, 849
Bst2UI CCWGG 1 cut(s) 699
Bst4CI ACNGT 3 cut(s) 136, 280, 366
BstACI GRCGYC 1 cut(s) 738
BstAUI TGTACA 1 cut(s) 282
BstC8I GCNNGC 3 cut(s) 346, 585, 599
BstDEI CTNAG 3 cut(s) 44, 125, 436
BstDSI CCRYGG 1 cut(s) 849
BstF5I GGATG 1 cut(s) 87
BstFNI CGCG 1 cut(s) 354
BstHHI GCGC 1 cut(s) 540
BstKTI GATC 2 cut(s) 631, 697
BstMAI GTCTC 2 cut(s) 434, 609
BstMBI GATC 2 cut(s) 628, 694
BstMCI CGRYCG 1 cut(s) 352
BstMWI GCNNNNNNNGC 1 cut(s) 49
BstNI CCWGG 1 cut(s) 699
BstNSI RCATGY 1 cut(s) 587
BstSCI CCNGG 1 cut(s) 697
BstSFI CTRYAG 1 cut(s) 742
BstUI CGCG 1 cut(s) 354
BstV1I GCAGC 1 cut(s) 84
BstX2I RGATCY 1 cut(s) 694
BstYI RGATCY 1 cut(s) 694
BtgI CCRYGG 1 cut(s) 849
BtsCI GGATG 1 cut(s) 87
BveI ACCTGC 1 cut(s) 265
Cac8I GCNNGC 3 cut(s) 346, 585, 599
CfoI GCGC 1 cut(s) 540
Cfr10I RCCGGY 1 cut(s) 197
Cfr13I GGNCC 1 cut(s) 757
Csp6I GTAC 3 cut(s) 4, 276, 283
CviAII CATG 4 cut(s) 209, 528, 584, 850
CviJI RGCY 9 cut(s) 27, 52, 253, 486, 569, 717, 747, 769, 825
CviKI_1 RGCY 9 cut(s) 27, 52, 253, 486, 569, 717, 747, 769, 825
CviQI GTAC 3 cut(s) 4, 276, 283
DdeI CTNAG 3 cut(s) 44, 125, 436
DpnI GATC 2 cut(s) 630, 696
DpnII GATC 2 cut(s) 628, 694
DriI GACNNNNNGTC 1 cut(s) 622
Eam1105I GACNNNNNGTC 1 cut(s) 622
Ecl136II GAGCTC 2 cut(s) 569, 769
Eco130I CCWWGG 3 cut(s) 104, 236, 849
Eco24I GRGCYC 2 cut(s) 571, 771
Eco47I GGWCC 1 cut(s) 757
Eco53kI GAGCTC 2 cut(s) 569, 769
Eco57I CTGAAG 1 cut(s) 60
EcoICRI GAGCTC 2 cut(s) 569, 769
EcoRI GAATTC 1 cut(s) 467
EcoRII CCWGG 1 cut(s) 697
EcoT14I CCWWGG 3 cut(s) 104, 236, 849
EcoT22I ATGCAT 1 cut(s) 589
EcoT38I GRGCYC 2 cut(s) 571, 771
ErhI CCWWGG 3 cut(s) 104, 236, 849
FaeI CATG 4 cut(s) 212, 531, 587, 853
FalI AAGNNNNNCTT 2 cut(s) 438, 470
FaqI GGGAC 1 cut(s) 195
FatI CATG 4 cut(s) 208, 527, 583, 849
Fnu4HI GCNGC 1 cut(s) 98
FokI GGATG 1 cut(s) 94
FriOI GRGCYC 2 cut(s) 571, 771
Fsp4HI GCNGC 1 cut(s) 98
FspBI CTAG 2 cut(s) 668, 827
GlaI GCGC 1 cut(s) 539
GluI GCNGC 1 cut(s) 98
HapII CCGG 2 cut(s) 198, 564
HhaI GCGC 1 cut(s) 540
Hin1I GRCGYC 1 cut(s) 738
Hin1II CATG 4 cut(s) 212, 531, 587, 853
Hin6I GCGC 1 cut(s) 538
HinP1I GCGC 1 cut(s) 538
HinfI GANTC 1 cut(s) 845
HpaII CCGG 2 cut(s) 198, 564
HphI GGTGA 1 cut(s) 222
Hpy166II GTNNAC 1 cut(s) 283
Hpy188I TCNGA 4 cut(s) 47, 525, 628, 808
Hpy188III TCNNGA 5 cut(s) 155, 322, 614, 772, 796
Hpy8I GTNNAC 1 cut(s) 283
HpyCH4III ACNGT 3 cut(s) 136, 280, 366
HpyCH4IV ACGT 1 cut(s) 738
HpyCH4V TGCA 5 cut(s) 100, 344, 556, 583, 587
HpyF10VI GCNNNNNNNGC 1 cut(s) 49
HpyF3I CTNAG 3 cut(s) 44, 125, 436
HpySE526I ACGT 1 cut(s) 738
Hsp92I GRCGYC 1 cut(s) 738
Hsp92II CATG 4 cut(s) 212, 531, 587, 853
HspAI GCGC 1 cut(s) 538
Kzo9I GATC 2 cut(s) 628, 694
LmnI GCTCC 3 cut(s) 566, 677, 774
Lsp1109I GCAGC 1 cut(s) 84
LweI GCATC 2 cut(s) 331, 596
MaeI CTAG 2 cut(s) 668, 827
MaeII ACGT 1 cut(s) 738
MaeIII GTNAC 3 cut(s) 17, 228, 734
MalI GATC 2 cut(s) 630, 696
MboI GATC 2 cut(s) 628, 694
MboII GAAGA 4 cut(s) 66, 311, 824, 866
MflI RGATCY 1 cut(s) 694
MhlI GDGCHC 2 cut(s) 571, 771
MluCI AATT 9 cut(s) 188, 219, 267, 378, 422, 467, 546, 603, 719
MlyI GAGTC 1 cut(s) 854
MmeI TCCRAC 1 cut(s) 276
MnlI CCTC 2 cut(s) 243, 462
Mph1103I ATGCAT 1 cut(s) 589
MroXI GAANNNNTTC 2 cut(s) 147, 487
MseI TTAA 3 cut(s) 9, 222, 266
MslI CAYNNNNRTG 2 cut(s) 408, 510
MspI CCGG 2 cut(s) 198, 564
MspR9I CCNGG 1 cut(s) 699
MvaI CCWGG 1 cut(s) 699
MvnI CGCG 1 cut(s) 354
MwoI GCNNNNNNNGC 1 cut(s) 49
NcoI CCATGG 1 cut(s) 849
NdeII GATC 2 cut(s) 628, 694
NlaIII CATG 4 cut(s) 212, 531, 587, 853
NmuCI GTSAC 2 cut(s) 228, 734
NsiI ATGCAT 1 cut(s) 589
NspI RCATGY 1 cut(s) 587
PaeI GCATGC 1 cut(s) 587
PaqCI CACCTGC 1 cut(s) 265
PdmI GAANNNNTTC 2 cut(s) 147, 487
PfoI TCCNGGA 1 cut(s) 697
PkrI GCNGC 1 cut(s) 99
PleI GAGTC 1 cut(s) 853
PpsI GAGTC 1 cut(s) 853
Psp124BI GAGCTC 2 cut(s) 571, 771
Psp6I CCWGG 1 cut(s) 697
PspGI CCWGG 1 cut(s) 697
PspPI GGNCC 1 cut(s) 757
PsuI RGATCY 1 cut(s) 694
RsaI GTAC 3 cut(s) 5, 277, 284
RsaNI GTAC 3 cut(s) 4, 276, 283
RseI CAYNNNNRTG 2 cut(s) 408, 510
SacI GAGCTC 2 cut(s) 571, 771
SaqAI TTAA 3 cut(s) 9, 222, 266
SatI GCNGC 1 cut(s) 98
Sau3AI GATC 2 cut(s) 628, 694
Sau96I GGNCC 1 cut(s) 757
SchI GAGTC 1 cut(s) 854
ScrFI CCNGG 1 cut(s) 699
SduI GDGCHC 2 cut(s) 571, 771
SfaNI GCATC 2 cut(s) 331, 596
SfcI CTRYAG 1 cut(s) 742
SinI GGWCC 1 cut(s) 757
SmiMI CAYNNNNRTG 2 cut(s) 408, 510
SpeI ACTAGT 1 cut(s) 667
SphI GCATGC 1 cut(s) 587
Sse9I AATT 9 cut(s) 188, 219, 267, 378, 422, 467, 546, 603, 719
SsiI CCGC 1 cut(s) 352
SspI AATATT 1 cut(s) 309
SspMI CTAG 2 cut(s) 668, 827
SstI GAGCTC 2 cut(s) 571, 771
StyD4I CCNGG 1 cut(s) 697
StyI CCWWGG 3 cut(s) 104, 236, 849
TaaI ACNGT 3 cut(s) 136, 280, 366
TaiI ACGT 1 cut(s) 741
TaqI TCGA 2 cut(s) 32, 613
TasI AATT 9 cut(s) 188, 219, 267, 378, 422, 467, 546, 603, 719
TatI WGTACW 2 cut(s) 3, 282
Tru1I TTAA 3 cut(s) 9, 222, 266
Tru9I TTAA 3 cut(s) 9, 222, 266
TseFI GTSAC 2 cut(s) 228, 734
TseI GCWGC 1 cut(s) 97
Tsp45I GTSAC 2 cut(s) 228, 734
TspDTI ATGAA 9 cut(s) 140, 358, 390, 460, 480, 491, 509, 825, 829
TspGWI ACGGA 2 cut(s) 55, 740
VpaK11BI GGWCC 1 cut(s) 757
XapI RAATTY 2 cut(s) 467, 546
XceI RCATGY 1 cut(s) 587
XmnI GAANNNNTTC 2 cut(s) 147, 487
XspI CTAG 2 cut(s) 668, 827
ZraI GACGTC 1 cut(s) 739
Zsp2I ATGCAT 1 cut(s) 589
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.