FvH4_2g32480
HSP70 Family

Heat shock cognate 70 kDa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
N/A
Physical Location & Seq
Reverse (-)
24618736 .. 24620690
1955 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g32480.t1

Sequence Viewer

Length: 1158 bp
ATGGCTTTAGCGAGAAAAGGAGAGGATCCAGTTATTGGAATAGATCTTGGAACAACTTATTCTTGCGTCGGTGTTTGGCAACACGATCGCGTAGAGATCATAGCCAACGATCAAGGCAACAGAACCACACCTTCCTATGTTGCTTTCACTAATACAGAGCGTTTGATTGGTGATGCCGCTAAGAACCAAGCCGACGTCAACCCAATAAACACTGTTTTTGGCATAAAGAGATTGATTGGCAGGAAATTTAGTGATACATGTGTTAAGAGTGATATGAAATTATGGCCTTTCAAGGTTCGTGGTGTGGGGGACAAGCCCATGATTGTTATCGACTACAATAATGAAAAGAAGCAATTCACACCTGAGAAAATATCTTCAATGGTTCTTAGTAAGATGCGTGAAATAGCAGAGACCTACCTTGGAACGACTGCTACTGCGGGAGATACACATATTGGGGGTGAAGATTTTGATGATAGAATGGTGGACCATTTTGTGCAAGAGTTCAAGAAGAAGAAAATTGTTGACGATGTTGTCCTTGTTGGTGGATCTACTAGAATTCCCAAGGTACAACAATTATTGCATGAGTTCTTTAACGGTAAAGAGCTATGTAAGAGCATTAATTCTGACGAAGCAGTTGCGTATGGTGCTGCCCTACAAGCTGCAGTTTTGCGCGGTGGCATTGAGAAGGTGCAAGACTTACTCATGTTGGATGTAACTTCACATTCCCTTGGCTATATGCGGTACACAGGAGATTTTGATGTTTTTATCCCAAAAAACACAACCATTCCCACCAAGAAATCTAAAGTTTACACAACTATTGAACAAAACCAAACTGGTTTAATAGTTGATGTGTATGAGGGCGAAGAAGCAAGAGCTATAGACAACAAACTGTTGGGTCATTTTGTGCTTGATGGAATCCCCCCTGGTCCAAAAGGGAAACCTCTTGTTACTGTTAGCTACGAGATCGATGCCAATGGAATTTTAACTGTGACCGCAGAGGAAAAACGACTCCGACTTAAGAAGAAAATTGTAATCTCCGATTATAGTTACGGATTATCTAAAATGGAAATTGAGAAGATAAAGTTAGAAGATGAGGAACATAACAAGAGAGTGGAGGCCAGATATGCATTGGAAGACTGCTCAACTAATCAAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko04010 ko04141 ko04144 ko04213 ko04612 ko04915 ko05134 ko05145 ko05162 ko05164 ko05169 map03040 map04010 map04141 map04144 map04213 map04612 map04915 map05134 map05145 map05162 map05164 map05169
Pfam Domains
Protein Families
HSP70

Protein Analysis

386

Amino Acids

42.95

Weight (kDa)

6.06

Isoelectric Point (pI)

20.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 11 - 142 4.5e-50 Hsp70 protein
HSP70 PF00012 143 - 175 3.4e-08 Hsp70 protein
HSP70 PF00012 171 - 375 3.6e-64 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 530
AatII GACGTC 1 cut(s) 198
AccB7I CCANNNNNTGG 1 cut(s) 35
AccII CGCG 2 cut(s) 90, 672
AciI CCGC 5 cut(s) 177, 437, 672, 739, 993
AclWI GGATC 3 cut(s) 20, 33, 553
AcsI RAATTY 3 cut(s) 245, 555, 978
AcyI GRCGYC 1 cut(s) 195
AfaI GTAC 2 cut(s) 567, 743
AfiI CCNNNNNNNGG 1 cut(s) 35
AflII CTTAAG 1 cut(s) 1016
AflIII ACRYGT 1 cut(s) 257
AgsI TTSAA 4 cut(s) 292, 378, 505, 821
AjnI CCWGG 1 cut(s) 922
AluBI AGCT 4 cut(s) 604, 659, 875, 957
AluI AGCT 4 cut(s) 604, 659, 875, 957
Alw26I GTCTC 1 cut(s) 404
AlwI GGATC 3 cut(s) 20, 33, 553
AoxI GGCC 2 cut(s) 284, 1116
ApeKI GCWGC 2 cut(s) 647, 659
ApoI RAATTY 3 cut(s) 245, 555, 978
AseI ATTAAT 1 cut(s) 618
Asp700I GAANNNNTTC 1 cut(s) 353
AspLEI GCGC 1 cut(s) 672
AspS9I GGNCC 2 cut(s) 484, 926
AsuHPI GGTGA 2 cut(s) 182, 470
AvaII GGWCC 2 cut(s) 484, 926
BaeI ACNNNNGTAYC 2 cut(s) 246, 279
BamHI GGATCC 1 cut(s) 25
BbsI GAAGAC 1 cut(s) 1140
BbvI GCAGC 2 cut(s) 634, 646
BccI CCATC 1 cut(s) 905
BcgI CGANNNNNNTGC 6 cut(s) 68, 102, 617, 651, 950, 984
BciT130I CCWGG 1 cut(s) 924
BcoDI GTCTC 1 cut(s) 404
BfaI CTAG 1 cut(s) 552
BfmI CTRYAG 2 cut(s) 660, 876
BfrI CTTAAG 1 cut(s) 1016
BglII AGATCT 1 cut(s) 43
BisI GCNGC 3 cut(s) 177, 648, 660
BlsI GCNGC 3 cut(s) 178, 649, 661
Bme1390I CCNGG 1 cut(s) 924
Bme18I GGWCC 2 cut(s) 484, 926
BmgT120I GGNCC 2 cut(s) 484, 926
BmiI GGNNCC 1 cut(s) 27
BmrFI CCNGG 1 cut(s) 924
BmsI GCATC 3 cut(s) 163, 384, 958
BpiI GAAGAC 1 cut(s) 1140
Bsa29I ATCGAT 1 cut(s) 966
BsaBI GATNNNNATC 1 cut(s) 326
BsaHI GRCGYC 1 cut(s) 195
BsaI GGTCTC 1 cut(s) 404
BsaJI CCNNGG 4 cut(s) 418, 561, 727, 922
Bsc4I CCNNNNNNNGG 1 cut(s) 35
Bse1I ACTGG 2 cut(s) 29, 838
Bse8I GATNNNNATC 1 cut(s) 326
BseBI CCWGG 1 cut(s) 924
BseCI ATCGAT 1 cut(s) 966
BseDI CCNNGG 4 cut(s) 418, 561, 727, 922
BseGI GGATG 1 cut(s) 715
BseJI GATNNNNATC 1 cut(s) 326
BseLI CCNNNNNNNGG 1 cut(s) 35
BseMII CTCAG 1 cut(s) 354
BseNI ACTGG 2 cut(s) 29, 838
BseXI GCAGC 2 cut(s) 634, 646
Bsh1236I CGCG 2 cut(s) 90, 672
Bsh1285I CGRYCG 1 cut(s) 88
BshFI GGCC 2 cut(s) 286, 1118
BshVI ATCGAT 1 cut(s) 966
BsiEI CGRYCG 1 cut(s) 88
BslFI GGGAC 1 cut(s) 323
BslI CCNNNNNNNGG 1 cut(s) 35
BsmAI GTCTC 1 cut(s) 404
BsmFI GGGAC 1 cut(s) 323
BsnI GGCC 2 cut(s) 286, 1118
Bso31I GGTCTC 1 cut(s) 404
Bsp143I GATC 7 cut(s) 25, 43, 85, 96, 109, 545, 963
BspACI CCGC 5 cut(s) 177, 437, 672, 739, 993
BspANI GGCC 2 cut(s) 286, 1118
BspCNI CTCAG 1 cut(s) 355
BspDI ATCGAT 1 cut(s) 966
BspFNI CGCG 2 cut(s) 90, 672
BspLI GGNNCC 1 cut(s) 27
BspMAI CTGCAG 1 cut(s) 664
BspPI GGATC 3 cut(s) 20, 33, 553
BspTI CTTAAG 1 cut(s) 1016
BspTNI GGTCTC 1 cut(s) 404
BsrI ACTGG 2 cut(s) 29, 838
BssECI CCNNGG 4 cut(s) 418, 561, 727, 922
BssMI GATC 7 cut(s) 25, 43, 85, 96, 109, 545, 963
BssNI GRCGYC 1 cut(s) 195
BssT1I CCWWGG 3 cut(s) 418, 561, 727
Bst2UI CCWGG 1 cut(s) 924
Bst4CI ACNGT 5 cut(s) 214, 596, 891, 952, 988
BstACI GRCGYC 1 cut(s) 195
BstAFI CTTAAG 1 cut(s) 1016
BstDEI CTNAG 3 cut(s) 180, 363, 386
BstF5I GGATG 1 cut(s) 715
BstFNI CGCG 2 cut(s) 90, 672
BstHHI GCGC 1 cut(s) 672
BstKTI GATC 7 cut(s) 28, 46, 88, 99, 112, 548, 966
BstMAI GTCTC 1 cut(s) 404
BstMBI GATC 7 cut(s) 25, 43, 85, 96, 109, 545, 963
BstMCI CGRYCG 1 cut(s) 88
BstMWI GCNNNNNNNGC 3 cut(s) 644, 656, 1124
BstNI CCWGG 1 cut(s) 924
BstNSI RCATGY 1 cut(s) 261
BstSCI CCNGG 1 cut(s) 922
BstSFI CTRYAG 2 cut(s) 660, 876
BstUI CGCG 2 cut(s) 90, 672
BstV1I GCAGC 2 cut(s) 634, 646
BstV2I GAAGAC 1 cut(s) 1140
BstX2I RGATCY 3 cut(s) 25, 43, 545
BstYI RGATCY 3 cut(s) 25, 43, 545
Bsu15I ATCGAT 1 cut(s) 966
BsuRI GGCC 2 cut(s) 286, 1118
BsuTUI ATCGAT 1 cut(s) 966
BtsCI GGATG 1 cut(s) 715
BtsIMutI CAGTG 1 cut(s) 210
CfoI GCGC 1 cut(s) 672
Cfr13I GGNCC 2 cut(s) 484, 926
ClaI ATCGAT 1 cut(s) 966
CseI GACGC 1 cut(s) 55
Csp6I GTAC 2 cut(s) 566, 742
CspCI CAANNNNNGTGG 2 cut(s) 280, 315
CviAII CATG 4 cut(s) 258, 319, 581, 703
CviQI GTAC 2 cut(s) 566, 742
DdeI CTNAG 3 cut(s) 180, 363, 386
DpnI GATC 7 cut(s) 27, 45, 87, 98, 111, 547, 965
DpnII GATC 7 cut(s) 25, 43, 85, 96, 109, 545, 963
DrdI GACNNNNNNGTC 1 cut(s) 530
DseDI GACNNNNNNGTC 1 cut(s) 530
Eco130I CCWWGG 3 cut(s) 418, 561, 727
Eco31I GGTCTC 1 cut(s) 404
Eco47I GGWCC 2 cut(s) 484, 926
EcoRI GAATTC 1 cut(s) 555
EcoRII CCWGG 1 cut(s) 922
EcoT14I CCWWGG 3 cut(s) 418, 561, 727
EcoT22I ATGCAT 1 cut(s) 1129
ErhI CCWWGG 3 cut(s) 418, 561, 727
FaeI CATG 4 cut(s) 261, 322, 584, 706
FaqI GGGAC 1 cut(s) 323
FatI CATG 4 cut(s) 257, 318, 580, 702
FauI CCCGC 1 cut(s) 430
Fnu4HI GCNGC 3 cut(s) 177, 648, 660
FokI GGATG 1 cut(s) 722
Fsp4HI GCNGC 3 cut(s) 177, 648, 660
FspBI CTAG 1 cut(s) 552
GlaI GCGC 1 cut(s) 671
GluI GCNGC 3 cut(s) 177, 648, 660
HaeIII GGCC 2 cut(s) 286, 1118
HgaI GACGC 1 cut(s) 55
HhaI GCGC 1 cut(s) 672
Hin1I GRCGYC 1 cut(s) 195
Hin1II CATG 4 cut(s) 261, 322, 584, 706
Hin6I GCGC 1 cut(s) 670
HinP1I GCGC 1 cut(s) 670
HincII GTYRAC 2 cut(s) 199, 523
HindII GTYRAC 2 cut(s) 199, 523
HinfI GANTC 2 cut(s) 915, 1008
HphI GGTGA 2 cut(s) 182, 470
Hpy166II GTNNAC 5 cut(s) 199, 484, 523, 744, 808
Hpy188I TCNGA 3 cut(s) 625, 1013, 1039
Hpy188III TCNNGA 1 cut(s) 505
Hpy8I GTNNAC 5 cut(s) 199, 484, 523, 744, 808
Hpy99I CGWCG 2 cut(s) 71, 197
HpyAV CCTTC 2 cut(s) 141, 679
HpyCH4III ACNGT 5 cut(s) 214, 596, 891, 952, 988
HpyCH4IV ACGT 1 cut(s) 195
HpyCH4V TGCA 5 cut(s) 496, 580, 662, 691, 1127
HpyF10VI GCNNNNNNNGC 3 cut(s) 644, 656, 1124
HpyF3I CTNAG 3 cut(s) 180, 363, 386
HpySE526I ACGT 1 cut(s) 195
Hsp92I GRCGYC 1 cut(s) 195
Hsp92II CATG 4 cut(s) 261, 322, 584, 706
HspAI GCGC 1 cut(s) 670
Kzo9I GATC 7 cut(s) 25, 43, 85, 96, 109, 545, 963
LpnPI CCDG 8 cut(s) 42, 226, 375, 732, 819, 909, 936, 1132
Lsp1109I GCAGC 2 cut(s) 634, 646
LweI GCATC 3 cut(s) 163, 384, 958
MaeI CTAG 1 cut(s) 552
MaeII ACGT 1 cut(s) 195
MaeIII GTNAC 4 cut(s) 712, 946, 988, 1046
MalI GATC 7 cut(s) 27, 45, 87, 98, 111, 547, 965
MboI GATC 7 cut(s) 25, 43, 85, 96, 109, 545, 963
MboII GAAGA 9 cut(s) 366, 473, 520, 523, 875, 1033, 1087, 1100, 1145
MflI RGATCY 3 cut(s) 25, 43, 545
MlyI GAGTC 1 cut(s) 1002
MmeI TCCRAC 2 cut(s) 687, 1036
MnlI CCTC 6 cut(s) 16, 850, 951, 991, 1087, 1108
Mph1103I ATGCAT 1 cut(s) 1129
MroXI GAANNNNTTC 1 cut(s) 353
MseI TTAA 6 cut(s) 264, 591, 618, 839, 983, 1017
MspCI CTTAAG 1 cut(s) 1016
MspR9I CCNGG 1 cut(s) 924
MvaI CCWGG 1 cut(s) 924
MvnI CGCG 2 cut(s) 90, 672
MwoI GCNNNNNNNGC 3 cut(s) 644, 656, 1124
NdeII GATC 7 cut(s) 25, 43, 85, 96, 109, 545, 963
NlaIII CATG 4 cut(s) 261, 322, 584, 706
NlaIV GGNNCC 1 cut(s) 27
NmuCI GTSAC 1 cut(s) 988
NsiI ATGCAT 1 cut(s) 1129
NspI RCATGY 1 cut(s) 261
PciI ACATGT 1 cut(s) 257
PdmI GAANNNNTTC 1 cut(s) 353
PfeI GAWTC 1 cut(s) 915
PflMI CCANNNNNTGG 1 cut(s) 35
PkrI GCNGC 3 cut(s) 178, 649, 661
Ple19I CGATCG 1 cut(s) 88
PleI GAGTC 1 cut(s) 1002
PpsI GAGTC 1 cut(s) 1002
PscI ACATGT 1 cut(s) 257
PshBI ATTAAT 1 cut(s) 618
Psp6I CCWGG 1 cut(s) 922
PspGI CCWGG 1 cut(s) 922
PspN4I GGNNCC 1 cut(s) 27
PspPI GGNCC 2 cut(s) 484, 926
PsrI GAACNNNNNNTAC 2 cut(s) 415, 447
PstI CTGCAG 1 cut(s) 664
PsuI RGATCY 3 cut(s) 25, 43, 545
PvuI CGATCG 1 cut(s) 88
RsaI GTAC 2 cut(s) 567, 743
RsaNI GTAC 2 cut(s) 566, 742
SaqAI TTAA 6 cut(s) 264, 591, 618, 839, 983, 1017
SatI GCNGC 3 cut(s) 177, 648, 660
Sau3AI GATC 7 cut(s) 25, 43, 85, 96, 109, 545, 963
Sau96I GGNCC 2 cut(s) 484, 926
SchI GAGTC 1 cut(s) 1002
ScrFI CCNGG 1 cut(s) 924
SfaNI GCATC 3 cut(s) 163, 384, 958
SfcI CTRYAG 2 cut(s) 660, 876
SinI GGWCC 2 cut(s) 484, 926
SmlI CTYRAG 1 cut(s) 1016
SmoI CTYRAG 1 cut(s) 1016
SsiI CCGC 5 cut(s) 177, 437, 672, 739, 993
SspMI CTAG 1 cut(s) 552
StyD4I CCNGG 1 cut(s) 922
StyI CCWWGG 3 cut(s) 418, 561, 727
TaaI ACNGT 5 cut(s) 214, 596, 891, 952, 988
TaiI ACGT 1 cut(s) 198
TaqI TCGA 2 cut(s) 330, 966
TauI GCSGC 1 cut(s) 179
TfiI GAWTC 1 cut(s) 915
Tru1I TTAA 6 cut(s) 264, 591, 618, 839, 983, 1017
Tru9I TTAA 6 cut(s) 264, 591, 618, 839, 983, 1017
TscAI CASTG 1 cut(s) 217
TseFI GTSAC 1 cut(s) 988
TseI GCWGC 2 cut(s) 647, 659
Tsp45I GTSAC 1 cut(s) 988
TspDTI ATGAA 2 cut(s) 290, 357
TspGWI ACGGA 1 cut(s) 1065
TspRI CASTG 1 cut(s) 217
Van91I CCANNNNNTGG 1 cut(s) 35
Vha464I CTTAAG 1 cut(s) 1016
VpaK11BI GGWCC 2 cut(s) 484, 926
VspI ATTAAT 1 cut(s) 618
XapI RAATTY 3 cut(s) 245, 555, 978
XceI RCATGY 1 cut(s) 261
XcmI CCANNNNNNNNNTGG 1 cut(s) 1126
XmnI GAANNNNTTC 1 cut(s) 353
XspI CTAG 1 cut(s) 552
ZraI GACGTC 1 cut(s) 196
Zsp2I ATGCAT 1 cut(s) 1129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.