FvH4_6g41150
HSP70 Family

Heat shock cognate 70 kDa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
N/A
Physical Location & Seq
Reverse (-)
32427889 .. 32428873
985 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g41150.t1

Sequence Viewer

Length: 549 bp
ATGGAGATAATCAGTGATCATGATATTGTTTTTGCTTGTGGCTCTTCTAGAATTCCAAAGGTCCAGCAACTACTTCAAGGTAAAGAGTTATGCAAGGGCATCCATCCTGATGAGGCTATTGAGCAAGGTGCTGCTGTTCAAGCTGCCGTTTTGAATAGTAGCAATTTCTCTGGGAAGCTTCAAAATTCACTCTTTTGGATGGAAACAACGTTTGATAACAACTACTTGGGTGAATTTGATATTGATGGCATTCCTCCAGCTCCTGCGGGTGAAGCAAAATTTGATGTATGCTTCAACATTGATGCAAATGGAATCCTGAGTGTTACTGCTAAGGATATATCCACTGGCCGGGAGGAAGGGATCACAATTAACTGTTTCCAGAAGCTAACCATAGTAGAAAAGAGTGGCAAACAAAGAAAGAAACAAAATCCAACGCGGAAGAAGACCGAGAAAAATATTTCGGAAACAGACGAAAGACGGCGATTTTTTGTGGATGCCTTTCTACTGAGAGAAGCTGACAAGATATATGTACTTGACTTGACTTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko04010 ko04141 ko04144 ko04213 ko04612 ko04915 ko05134 ko05145 ko05162 ko05164 ko05169 map03040 map04010 map04141 map04144 map04213 map04612 map04915 map05134 map05145 map05162 map05164 map05169
Pfam Domains
Protein Families
HSP70

Protein Analysis

183

Amino Acids

20.39

Weight (kDa)

5.31

Isoelectric Point (pI)

39.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 7 - 53 1e-10 Hsp70 protein
HSP70 PF00012 69 - 155 4.2e-16 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 436
AciI CCGC 2 cut(s) 266, 436
AclI AACGTT 1 cut(s) 209
AclWI GGATC 1 cut(s) 368
AcoI YGGCCR 1 cut(s) 346
AcsI RAATTY 4 cut(s) 51, 184, 233, 278
AfaI GTAC 1 cut(s) 531
AfiI CCNNNNNNNGG 1 cut(s) 348
AgsI TTSAA 5 cut(s) 77, 140, 154, 182, 295
AluBI AGCT 5 cut(s) 143, 178, 260, 385, 515
AluI AGCT 5 cut(s) 143, 178, 260, 385, 515
AlwI GGATC 1 cut(s) 368
AlwNI CAGNNNCTG 1 cut(s) 263
AoxI GGCC 1 cut(s) 346
ApeKI GCWGC 2 cut(s) 131, 143
ApoI RAATTY 4 cut(s) 51, 184, 233, 278
AspS9I GGNCC 1 cut(s) 61
AsuC2I CCSGG 1 cut(s) 350
AsuHPI GGTGA 2 cut(s) 242, 281
AvaII GGWCC 1 cut(s) 61
BbsI GAAGAC 1 cut(s) 449
BbvI GCAGC 2 cut(s) 118, 130
BccI CCATC 3 cut(s) 111, 193, 239
BceAI ACGGC 2 cut(s) 131, 494
BclI TGATCA 1 cut(s) 16
BcnI CCSGG 1 cut(s) 350
BfaI CTAG 1 cut(s) 48
BisI GCNGC 2 cut(s) 132, 144
BlsI GCNGC 2 cut(s) 133, 145
Bme1390I CCNGG 1 cut(s) 350
Bme18I GGWCC 1 cut(s) 61
BmgT120I GGNCC 1 cut(s) 61
BmrFI CCNGG 1 cut(s) 350
BmsI GCATC 3 cut(s) 108, 292, 484
BpiI GAAGAC 1 cut(s) 449
BpmI CTGGAG 1 cut(s) 240
Bpu10I CCTNAGC 1 cut(s) 330
BpuMI CCSGG 1 cut(s) 350
Bsc4I CCNNNNNNNGG 1 cut(s) 348
Bse1I ACTGG 1 cut(s) 349
BseGI GGATG 4 cut(s) 99, 103, 204, 499
BseLI CCNNNNNNNGG 1 cut(s) 348
BseMII CTCAG 2 cut(s) 308, 497
BseNI ACTGG 1 cut(s) 349
BseXI GCAGC 2 cut(s) 118, 130
Bsh1236I CGCG 1 cut(s) 436
BshFI GGCC 1 cut(s) 348
BsiSI CCGG 1 cut(s) 349
BslI CCNNNNNNNGG 1 cut(s) 348
BsmI GAATGC 1 cut(s) 249
BsnI GGCC 1 cut(s) 348
Bsp143I GATC 2 cut(s) 16, 360
BspACI CCGC 2 cut(s) 266, 436
BspANI GGCC 1 cut(s) 348
BspCNI CTCAG 2 cut(s) 309, 498
BspFNI CGCG 1 cut(s) 436
BspHI TCATGA 1 cut(s) 19
BspPI GGATC 1 cut(s) 368
BspQI GCTCTTC 1 cut(s) 49
BsrI ACTGG 1 cut(s) 349
BssMI GATC 2 cut(s) 16, 360
Bst4CI ACNGT 1 cut(s) 374
Bst6I CTCTTC 1 cut(s) 49
BstDEI CTNAG 3 cut(s) 317, 330, 506
BstF5I GGATG 4 cut(s) 99, 103, 204, 499
BstFNI CGCG 1 cut(s) 436
BstKTI GATC 2 cut(s) 19, 363
BstMBI GATC 2 cut(s) 16, 360
BstMWI GCNNNNNNNGC 2 cut(s) 140, 272
BstSCI CCNGG 1 cut(s) 348
BstUI CGCG 1 cut(s) 436
BstV1I GCAGC 2 cut(s) 118, 130
BstV2I GAAGAC 1 cut(s) 449
BsuRI GGCC 1 cut(s) 348
BtsCI GGATG 4 cut(s) 99, 103, 204, 499
BtsIMutI CAGTG 2 cut(s) 19, 342
CaiI CAGNNNCTG 1 cut(s) 263
CciI TCATGA 1 cut(s) 19
Cfr13I GGNCC 1 cut(s) 61
Csp6I GTAC 1 cut(s) 530
CviAII CATG 1 cut(s) 20
CviJI RGCY 8 cut(s) 42, 116, 143, 178, 260, 348, 385, 515
CviKI_1 RGCY 8 cut(s) 42, 116, 143, 178, 260, 348, 385, 515
CviQI GTAC 1 cut(s) 530
DdeI CTNAG 3 cut(s) 317, 330, 506
DpnI GATC 2 cut(s) 18, 362
DpnII GATC 2 cut(s) 16, 360
EaeI YGGCCR 1 cut(s) 346
Eam1104I CTCTTC 1 cut(s) 49
EarI CTCTTC 1 cut(s) 49
Eco47I GGWCC 1 cut(s) 61
EcoRI GAATTC 1 cut(s) 51
FaeI CATG 1 cut(s) 23
FaiI YATR 7 cut(s) 21, 91, 289, 338, 392, 526, 528
FatI CATG 1 cut(s) 19
FauI CCCGC 1 cut(s) 259
FbaI TGATCA 1 cut(s) 16
Fnu4HI GCNGC 2 cut(s) 132, 144
FokI GGATG 4 cut(s) 86, 90, 211, 506
Fsp4HI GCNGC 2 cut(s) 132, 144
FspBI CTAG 1 cut(s) 48
GluI GCNGC 2 cut(s) 132, 144
GsuI CTGGAG 1 cut(s) 240
HaeIII GGCC 1 cut(s) 348
HapII CCGG 1 cut(s) 349
Hin1II CATG 1 cut(s) 23
HindIII AAGCTT 1 cut(s) 176
HinfI GANTC 1 cut(s) 312
HpaII CCGG 1 cut(s) 349
HphI GGTGA 2 cut(s) 242, 281
Hpy188I TCNGA 1 cut(s) 463
Hpy188III TCNNGA 5 cut(s) 20, 48, 107, 316, 379
HpyAV CCTTC 1 cut(s) 350
HpyCH4III ACNGT 1 cut(s) 374
HpyCH4IV ACGT 1 cut(s) 209
HpyCH4V TGCA 2 cut(s) 93, 305
HpyF10VI GCNNNNNNNGC 2 cut(s) 140, 272
HpyF3I CTNAG 3 cut(s) 317, 330, 506
HpySE526I ACGT 1 cut(s) 209
Hsp92II CATG 1 cut(s) 23
Ksp22I TGATCA 1 cut(s) 16
Kzo9I GATC 2 cut(s) 16, 360
LguI GCTCTTC 1 cut(s) 49
LmnI GCTCC 1 cut(s) 265
LpnPI CCDG 9 cut(s) 77, 120, 156, 270, 276, 329, 330, 362, 392
Lsp1109I GCAGC 2 cut(s) 118, 130
LweI GCATC 3 cut(s) 108, 292, 484
MaeI CTAG 1 cut(s) 48
MaeII ACGT 1 cut(s) 209
MaeIII GTNAC 1 cut(s) 322
MalI GATC 2 cut(s) 18, 362
MboI GATC 2 cut(s) 16, 360
MboII GAAGA 3 cut(s) 36, 451, 454
MluCI AATT 6 cut(s) 51, 163, 184, 233, 278, 366
MmeI TCCRAC 1 cut(s) 455
MnlI CCTC 3 cut(s) 106, 264, 346
MseI TTAA 1 cut(s) 369
MslI CAYNNNNRTG 1 cut(s) 108
MspI CCGG 1 cut(s) 349
MspR9I CCNGG 1 cut(s) 350
Mva1269I GAATGC 1 cut(s) 249
MvnI CGCG 1 cut(s) 436
MwoI GCNNNNNNNGC 2 cut(s) 140, 272
NciI CCSGG 1 cut(s) 350
NdeII GATC 2 cut(s) 16, 360
NlaIII CATG 1 cut(s) 23
PagI TCATGA 1 cut(s) 19
PciSI GCTCTTC 1 cut(s) 49
PctI GAATGC 1 cut(s) 249
PfeI GAWTC 1 cut(s) 312
PkrI GCNGC 2 cut(s) 133, 145
Psp1406I AACGTT 1 cut(s) 209
PspPI GGNCC 1 cut(s) 61
PstNI CAGNNNCTG 1 cut(s) 263
RsaI GTAC 1 cut(s) 531
RsaNI GTAC 1 cut(s) 530
RseI CAYNNNNRTG 1 cut(s) 108
SapI GCTCTTC 1 cut(s) 49
SaqAI TTAA 1 cut(s) 369
SatI GCNGC 2 cut(s) 132, 144
Sau3AI GATC 2 cut(s) 16, 360
Sau96I GGNCC 1 cut(s) 61
ScrFI CCNGG 1 cut(s) 350
SetI ASST 9 cut(s) 63, 82, 130, 145, 180, 212, 262, 387, 517
SfaNI GCATC 3 cut(s) 108, 292, 484
SinI GGWCC 1 cut(s) 61
SmiMI CAYNNNNRTG 1 cut(s) 108
Sse9I AATT 6 cut(s) 51, 163, 184, 233, 278, 366
SsiI CCGC 2 cut(s) 266, 436
SspI AATATT 1 cut(s) 457
SspMI CTAG 1 cut(s) 48
StyD4I CCNGG 1 cut(s) 348
TaaI ACNGT 1 cut(s) 374
TaiI ACGT 1 cut(s) 212
TaqII GACCGA 1 cut(s) 461
TasI AATT 6 cut(s) 51, 163, 184, 233, 278, 366
TatI WGTACW 1 cut(s) 529
TfiI GAWTC 1 cut(s) 312
Tru1I TTAA 1 cut(s) 369
Tru9I TTAA 1 cut(s) 369
TscAI CASTG 2 cut(s) 19, 349
TseI GCWGC 2 cut(s) 131, 143
TspRI CASTG 2 cut(s) 19, 349
VpaK11BI GGWCC 1 cut(s) 61
XapI RAATTY 4 cut(s) 51, 184, 233, 278
XbaI TCTAGA 1 cut(s) 47
XspI CTAG 1 cut(s) 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.