Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

N/A

Physical Location & Seq

Genomic Coordinates Reverse (-)

41075431 .. 41076306

Gene Structure 876 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1176700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 876 bp

ATGTCTGAGGTGGACGAGGAGTCGAAGCAGGTCTCGTACAAGGTCGTAAGGGATGCGAATGGGAATGTCAAGATCGACTGCCCTGTTATCGGCAAGCAGTTCGCCGCTGAAGAAATTTCAGCTCAGGTGTTGAGGAAGCTTGTGGATGATGCTTCAAAGTTTTTGAATGATAAAGTTGGTAAAGCAGTGATCACTGTGCCTGCTTACTTTAATGACTCTCAAAGAACTGCTACAAAAGATGCCGGCCGTATTGCTGGTTTGGAAGTTCTTAGGATTATAAATGAACCTACTGCTGCTTCTTTAGCATACGGCTTTGACAGGAAGAACAATGAAACTATCTTGGTATTTGACCTTGGAGGTGGTACTTTCGATGTTTCAGTACTTGAGGTTGGTGATGGTGTGTTTGAAGTGCTATCTATTTCAGGAGATACGCATTTGGGGGGCAAAACCGTCAACAGCACTAGGAAGACTTCAGGAGTCATTGTTTGTGGAAGTGGGATGAATTTAGTGTTTCTGGGAACTGAGGTTGGTCCATGGAGTAAAACTGGTGGACTTGGTGATGTTCTTGGAGGCCCCCCACCGGCTATGGCTGCAAACGGGCATCGAGTGATGACGATTTCTCCGCGTTATGATCAGTACAAGGATGCATGGGGTACAGAAGTCACAGTTGAGCTTAAAGTGGGAGATAAAACCGAAACAGTTCGATTTTTTTTTTTCACTGCTACAAACGAGGAGTTGATCGTGTTTTCGTGGATCACCCATTGTTTCTCGAGAGGGTATAGGGGAAAACTGCATCCAAAATTTATGGTCCGGTCGCTGGAGTCGATTTCAAGGACAATCAACGTAGTTTTAGCCTTCTATACCAGGCAGCTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0006605 GO:0006810 GO:0006886 GO:0006950 GO:0008104 GO:0008150 GO:0009266 GO:0009408 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009579 GO:0009628 GO:0009941 GO:0010035 GO:0010038 GO:0015031 GO:0015833 GO:0031967 GO:0031975 GO:0033036 GO:0033365 GO:0034613 GO:0042221 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045036 GO:0045184 GO:0046686 GO:0046907 GO:0048046 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070727 GO:0071702 GO:0071705 GO:0072594 GO:0072596 GO:0072598

KEGG Pathways

Metabolic & Signaling

ko03040 ko04010 ko04141 ko04144 ko04213 ko04612 ko04915 ko05134 ko05145 ko05162 ko05164 ko05169 map03040 map04010 map04141 map04144 map04213 map04612 map04915 map05134 map05145 map05162 map05164 map05169

Pfam Domains

Protein Families

HSP70

Protein Analysis

292

Amino Acids

31.72

Weight (kDa)

6.46

Isoelectric Point (pI)

25.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HSP70	PF00012	3 - 151	5.3e-68	Hsp70 protein
MreB_Mbl	PF06723	14 - 144	8.8e-12	MreB/Mbl protein
Glyco_transf_5	PF08323	169 - 238	3.5e-13	Starch synthase catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	278
Acc36I	ACCTGC	1 cut(s)	19
AccII	CGCG	1 cut(s)	625
AciI	CCGC	2 cut(s)	105, 623
AclWI	GGATC	1 cut(s)	761
AcoI	YGGCCR	1 cut(s)	244
AcsI	RAATTY	3 cut(s)	114, 502, 800
AcuI	CTGAAG	2 cut(s)	129, 456
AfaI	GTAC	5 cut(s)	38, 364, 381, 638, 655
AfiI	CCNNNNNNNGG	3 cut(s)	89, 580, 817
AgsI	TTSAA	4 cut(s)	156, 166, 407, 831
AhdI	GACNNNNNGTC	1 cut(s)	19
AjnI	CCWGG	1 cut(s)	863
AluBI	AGCT	4 cut(s)	122, 139, 673, 871
AluI	AGCT	4 cut(s)	122, 139, 673, 871
Alw26I	GTCTC	1 cut(s)	37
AlwI	GGATC	1 cut(s)	761
Ama87I	CYCGRG	1 cut(s)	769
AoxI	GGCC	2 cut(s)	244, 571
ApeKI	GCWGC	3 cut(s)	293, 590, 868
ApoI	RAATTY	3 cut(s)	114, 502, 800
Asp700I	GAANNNNTTC	1 cut(s)	699
AspS9I	GGNCC	3 cut(s)	530, 572, 808
AsuHPI	GGTGA	3 cut(s)	404, 569, 748
AvaI	CYCGRG	1 cut(s)	769
AvaII	GGWCC	2 cut(s)	530, 808
BaeI	ACNNNNGTAYC	2 cut(s)	645, 678
BbsI	GAAGAC	1 cut(s)	473
BbvI	GCAGC	2 cut(s)	280, 577
BccI	CCATC	1 cut(s)	389
BceAI	ACGGC	2 cut(s)	231, 325
BcgI	CGANNNNNNTGC	2 cut(s)	82, 116
BciT130I	CCWGG	1 cut(s)	865
BclI	TGATCA	2 cut(s)	189, 631
BcoDI	GTCTC	1 cut(s)	37
BfaI	CTAG	2 cut(s)	462, 874
BfuAI	ACCTGC	1 cut(s)	19
BisI	GCNGC	4 cut(s)	105, 294, 591, 869
BlsI	GCNGC	4 cut(s)	106, 295, 592, 870
BmcAI	AGTACT	1 cut(s)	381
Bme1390I	CCNGG	1 cut(s)	865
Bme18I	GGWCC	2 cut(s)	530, 808
BmeRI	GACNNNNNGTC	1 cut(s)	19
BmeT110I	CYCGRG	1 cut(s)	769
BmgT120I	GGNCC	3 cut(s)	530, 572, 808
BmiI	GGNNCC	1 cut(s)	574
BmrFI	CCNGG	1 cut(s)	865
BmsI	GCATC	6 cut(s)	43, 139, 229, 610, 634, 802
BpiI	GAAGAC	1 cut(s)	473
BpmI	CTGGAG	1 cut(s)	839
Bpu10I	CCTNAGC	1 cut(s)	123
BpuEI	CTTGAG	1 cut(s)	404
BsaI	GGTCTC	1 cut(s)	37
BsaJI	CCNNGG	2 cut(s)	352, 533
BsaWI	WCCGGW	1 cut(s)	810
BsaXI	ACNNNNNCTCC	4 cut(s)	468, 498, 604, 634
Bsc4I	CCNNNNNNNGG	3 cut(s)	89, 580, 817
Bse118I	RCCGGY	2 cut(s)	242, 580
Bse1I	ACTGG	1 cut(s)	550
BseBI	CCWGG	1 cut(s)	865
BseDI	CCNNGG	2 cut(s)	352, 533
BseGI	GGATG	5 cut(s)	58, 151, 504, 649, 793
BseLI	CCNNNNNNNGG	3 cut(s)	89, 580, 817
BseMII	CTCAG	2 cut(s)	137, 513
BseNI	ACTGG	1 cut(s)	550
BseRI	GAGGAG	2 cut(s)	32, 746
BseX3I	CGGCCG	1 cut(s)	244
BseXI	GCAGC	2 cut(s)	280, 577
Bsh1236I	CGCG	1 cut(s)	625
Bsh1285I	CGRYCG	2 cut(s)	247, 815
BshFI	GGCC	2 cut(s)	246, 573
BsiEI	CGRYCG	2 cut(s)	247, 815
BsiHKCI	CYCGRG	1 cut(s)	769
BsiSI	CCGG	3 cut(s)	243, 581, 811
BslI	CCNNNNNNNGG	3 cut(s)	89, 580, 817
BsmAI	GTCTC	1 cut(s)	37
BsnI	GGCC	2 cut(s)	246, 573
Bso31I	GGTCTC	1 cut(s)	37
BsoBI	CYCGRG	1 cut(s)	769
Bsp143I	GATC	5 cut(s)	72, 189, 631, 738, 753
Bsp19I	CCATGG	1 cut(s)	533
BspACI	CCGC	2 cut(s)	105, 623
BspANI	GGCC	2 cut(s)	246, 573
BspCNI	CTCAG	2 cut(s)	136, 514
BspFNI	CGCG	1 cut(s)	625
BspLI	GGNNCC	1 cut(s)	574
BspMI	ACCTGC	1 cut(s)	19
BspPI	GGATC	1 cut(s)	761
BspTNI	GGTCTC	1 cut(s)	37
BsrFI	RCCGGY	2 cut(s)	242, 580
BsrI	ACTGG	1 cut(s)	550
BssAI	RCCGGY	2 cut(s)	242, 580
BssECI	CCNNGG	2 cut(s)	352, 533
BssMI	GATC	5 cut(s)	72, 189, 631, 738, 753
BssT1I	CCWWGG	2 cut(s)	352, 533
Bst2UI	CCWGG	1 cut(s)	865
Bst4CI	ACNGT	4 cut(s)	196, 451, 667, 700
BstC8I	GCNNGC	3 cut(s)	95, 201, 244
BstDEI	CTNAG	4 cut(s)	6, 123, 269, 522
BstDSI	CCRYGG	1 cut(s)	533
BstF5I	GGATG	5 cut(s)	58, 151, 504, 649, 793
BstFNI	CGCG	1 cut(s)	625
BstKTI	GATC	5 cut(s)	75, 192, 634, 741, 756
BstMAI	GTCTC	1 cut(s)	37
BstMBI	GATC	5 cut(s)	72, 189, 631, 738, 753
BstMCI	CGRYCG	2 cut(s)	247, 815
BstMWI	GCNNNNNNNGC	2 cut(s)	302, 590
BstNI	CCWGG	1 cut(s)	865
BstSCI	CCNGG	1 cut(s)	863
BstUI	CGCG	1 cut(s)	625
BstV1I	GCAGC	2 cut(s)	280, 577
BstV2I	GAAGAC	1 cut(s)	473
BstZI	CGGCCG	1 cut(s)	244
BsuRI	GGCC	2 cut(s)	246, 573
BtgI	CCRYGG	1 cut(s)	533
BtsCI	GGATG	5 cut(s)	58, 151, 504, 649, 793
BtsI	GCAGTG	2 cut(s)	192, 717
BtsIMutI	CAGTG	3 cut(s)	192, 192, 717
BveI	ACCTGC	1 cut(s)	19
Cac8I	GCNNGC	3 cut(s)	95, 201, 244
Cfr10I	RCCGGY	2 cut(s)	242, 580
Cfr13I	GGNCC	3 cut(s)	530, 572, 808
Csp6I	GTAC	5 cut(s)	37, 363, 380, 637, 654
CviAII	CATG	2 cut(s)	534, 648
CviQI	GTAC	5 cut(s)	37, 363, 380, 637, 654
DdeI	CTNAG	4 cut(s)	6, 123, 269, 522
DpnI	GATC	5 cut(s)	74, 191, 633, 740, 755
DpnII	GATC	5 cut(s)	72, 189, 631, 738, 753
DriI	GACNNNNNGTC	1 cut(s)	19
EaeI	YGGCCR	1 cut(s)	244
EagI	CGGCCG	1 cut(s)	244
Eam1105I	GACNNNNNGTC	1 cut(s)	19
EclXI	CGGCCG	1 cut(s)	244
Eco130I	CCWWGG	2 cut(s)	352, 533
Eco31I	GGTCTC	1 cut(s)	37
Eco47I	GGWCC	2 cut(s)	530, 808
Eco52I	CGGCCG	1 cut(s)	244
Eco57I	CTGAAG	2 cut(s)	129, 456
Eco88I	CYCGRG	1 cut(s)	769
EcoO109I	RGGNCCY	1 cut(s)	572
EcoRII	CCWGG	1 cut(s)	863
EcoT14I	CCWWGG	2 cut(s)	352, 533
EcoT22I	ATGCAT	1 cut(s)	649
ErhI	CCWWGG	2 cut(s)	352, 533
FaeI	CATG	2 cut(s)	537, 651
FaiI	YATR	9 cut(s)	278, 307, 535, 587, 630, 649, 780, 806, 861
FatI	CATG	2 cut(s)	533, 647
FbaI	TGATCA	2 cut(s)	189, 631
Fnu4HI	GCNGC	4 cut(s)	105, 294, 591, 869
FokI	GGATG	5 cut(s)	65, 158, 511, 656, 780
Fsp4HI	GCNGC	4 cut(s)	105, 294, 591, 869
FspBI	CTAG	2 cut(s)	462, 874
GluI	GCNGC	4 cut(s)	105, 294, 591, 869
GsuI	CTGGAG	1 cut(s)	839
HaeIII	GGCC	2 cut(s)	246, 573
HapII	CCGG	3 cut(s)	243, 581, 811
Hin1II	CATG	2 cut(s)	537, 651
HincII	GTYRAC	1 cut(s)	454
HindII	GTYRAC	1 cut(s)	454
HindIII	AAGCTT	1 cut(s)	137
HinfI	GANTC	4 cut(s)	20, 215, 477, 821
HpaII	CCGG	3 cut(s)	243, 581, 811
HphI	GGTGA	3 cut(s)	404, 569, 748
Hpy166II	GTNNAC	3 cut(s)	13, 454, 551
Hpy188I	TCNGA	1 cut(s)	7
Hpy188III	TCNNGA	5 cut(s)	70, 423, 474, 769, 771
Hpy8I	GTNNAC	3 cut(s)	13, 454, 551
HpyAV	CCTTC	1 cut(s)	865
HpyCH4III	ACNGT	4 cut(s)	196, 451, 667, 700
HpyCH4IV	ACGT	1 cut(s)	843
HpyCH4V	TGCA	3 cut(s)	593, 647, 793
HpyF10VI	GCNNNNNNNGC	2 cut(s)	302, 590
HpyF3I	CTNAG	4 cut(s)	6, 123, 269, 522
HpySE526I	ACGT	1 cut(s)	843
Hsp92II	CATG	2 cut(s)	537, 651
KroI	GCCGGC	1 cut(s)	242
KroNI	GCCGGC	1 cut(s)	244
Ksp22I	TGATCA	2 cut(s)	189, 631
Kzo9I	GATC	5 cut(s)	72, 189, 631, 738, 753
Lsp1109I	GCAGC	2 cut(s)	280, 577
LweI	GCATC	6 cut(s)	43, 139, 229, 610, 634, 802
MaeI	CTAG	2 cut(s)	462, 874
MaeII	ACGT	1 cut(s)	843
MaeIII	GTNAC	1 cut(s)	661
MalI	GATC	5 cut(s)	74, 191, 633, 740, 755
MboI	GATC	5 cut(s)	72, 189, 631, 738, 753
MboII	GAAGA	3 cut(s)	122, 334, 478
MluCI	AATT	3 cut(s)	114, 502, 800
MlyI	GAGTC	4 cut(s)	29, 209, 486, 830
MnlI	CCTC	8 cut(s)	10, 126, 350, 379, 517, 563, 724, 767
Mph1103I	ATGCAT	1 cut(s)	649
MroNI	GCCGGC	1 cut(s)	242
MroXI	GAANNNNTTC	1 cut(s)	699
MseI	TTAA	2 cut(s)	210, 675
MspA1I	CMGCKG	1 cut(s)	107
MspI	CCGG	3 cut(s)	243, 581, 811
MspR9I	CCNGG	1 cut(s)	865
MvaI	CCWGG	1 cut(s)	865
MvnI	CGCG	1 cut(s)	625
MwoI	GCNNNNNNNGC	2 cut(s)	302, 590
NaeI	GCCGGC	1 cut(s)	244
NcoI	CCATGG	1 cut(s)	533
NdeII	GATC	5 cut(s)	72, 189, 631, 738, 753
NgoMIV	GCCGGC	1 cut(s)	242
NlaIII	CATG	2 cut(s)	537, 651
NlaIV	GGNNCC	1 cut(s)	574
NmuCI	GTSAC	1 cut(s)	661
NsiI	ATGCAT	1 cut(s)	649
PaeR7I	CTCGAG	1 cut(s)	769
PcsI	WCGNNNNNNNCGW	1 cut(s)	821
PdiI	GCCGGC	1 cut(s)	244
PdmI	GAANNNNTTC	1 cut(s)	699
PkrI	GCNGC	4 cut(s)	106, 295, 592, 870
PleI	GAGTC	4 cut(s)	28, 209, 485, 829
PpsI	GAGTC	4 cut(s)	28, 209, 485, 829
PsiI	TTATAA	1 cut(s)	278
Psp6I	CCWGG	1 cut(s)	863
PspGI	CCWGG	1 cut(s)	863
PspN4I	GGNNCC	1 cut(s)	574
PspPI	GGNCC	3 cut(s)	530, 572, 808
RsaI	GTAC	5 cut(s)	38, 364, 381, 638, 655
RsaNI	GTAC	5 cut(s)	37, 363, 380, 637, 654
SaqAI	TTAA	2 cut(s)	210, 675
SatI	GCNGC	4 cut(s)	105, 294, 591, 869
Sau3AI	GATC	5 cut(s)	72, 189, 631, 738, 753
Sau96I	GGNCC	3 cut(s)	530, 572, 808
ScaI	AGTACT	1 cut(s)	381
SchI	GAGTC	4 cut(s)	29, 209, 486, 830
ScrFI	CCNGG	1 cut(s)	865
SfaNI	GCATC	6 cut(s)	43, 139, 229, 610, 634, 802
Sfr274I	CTCGAG	1 cut(s)	769
SinI	GGWCC	2 cut(s)	530, 808
SlaI	CTCGAG	1 cut(s)	769
SmlI	CTYRAG	2 cut(s)	383, 769
SmoI	CTYRAG	2 cut(s)	383, 769
Sse9I	AATT	3 cut(s)	114, 502, 800
SsiI	CCGC	2 cut(s)	105, 623
SspMI	CTAG	2 cut(s)	462, 874
StyD4I	CCNGG	1 cut(s)	863
StyI	CCWWGG	2 cut(s)	352, 533
TaaI	ACNGT	4 cut(s)	196, 451, 667, 700
TaiI	ACGT	1 cut(s)	846
TaqI	TCGA	7 cut(s)	23, 75, 369, 604, 703, 770, 824
TasI	AATT	3 cut(s)	114, 502, 800
TatI	WGTACW	2 cut(s)	379, 636
TauI	GCSGC	1 cut(s)	107
Tru1I	TTAA	2 cut(s)	210, 675
Tru9I	TTAA	2 cut(s)	210, 675
TscAI	CASTG	3 cut(s)	192, 199, 724
TseFI	GTSAC	1 cut(s)	661
TseI	GCWGC	3 cut(s)	293, 590, 868
Tsp45I	GTSAC	1 cut(s)	661
TspDTI	ATGAA	3 cut(s)	297, 345, 515
TspRI	CASTG	3 cut(s)	192, 199, 724
VpaK11BI	GGWCC	2 cut(s)	530, 808
XapI	RAATTY	3 cut(s)	114, 502, 800
XhoI	CTCGAG	1 cut(s)	769
XmnI	GAANNNNTTC	1 cut(s)	699
XspI	CTAG	2 cut(s)	462, 874
ZrmI	AGTACT	1 cut(s)	381
Zsp2I	ATGCAT	1 cut(s)	649

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.