MD01G1129900.v1.1
HSP70 Family

Belongs to the heat shock protein 70 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
N/A
Physical Location & Seq
Forward (+)
24059958 .. 24060486
529 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1129900.v1.1.491

Sequence Viewer

Length: 462 bp
ATGGCTCACGCAGACATGAATAGAACACTGGATTCCAGGGGAAACATAATTTCTTTGAGGATGTTCTCTGTTTACTCCATACGCGCACTCACCCTGAGCTCTGTTAACGTGTTATTTTCAGATTCAAAGTGTTTAATAGGAAGGAGATTTAGTGATCCTTCTGTTCAAAATTATAGGAAGCTGTGGCCATTCAAGGTCACTGAAGGTCCAGCTGAGAAGCCCATGATTGTGATGCGGGAGATTGCCGAGGCCTATCTCGGCTCAACTGTGAAGAATGCTGTGATCAAAGTCCCTGCTTACTTCACTGACTCGCAGCTTCAGGCTACAAAAGATGCTGGTACCACGGTTGGCCTAAATGTCATCCAATCATCAAAGAACCAACTGTGGCAGCGACTTGACAAGAAACCCGGTTGGTATAGCAAGAGAAACGTCAAGATGTTTGATTTGGGTGGTGCTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko04010 ko04141 ko04144 ko04213 ko04612 ko04915 ko05134 ko05145 ko05162 ko05164 ko05169 map03040 map04010 map04141 map04144 map04213 map04612 map04915 map05134 map05145 map05162 map05164 map05169
Pfam Domains
Protein Families
HSP70

Protein Analysis

154

Amino Acids

17.18

Weight (kDa)

9.95

Isoelectric Point (pI)

39.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 78 - 153 3e-16 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 338
AccB1I GGYRCC 1 cut(s) 338
AccII CGCG 1 cut(s) 84
AciI CCGC 1 cut(s) 235
AclWI GGATC 1 cut(s) 149
AcoI YGGCCR 1 cut(s) 185
AcuI CTGAAG 2 cut(s) 222, 302
AfaI GTAC 1 cut(s) 340
AflIII ACRYGT 1 cut(s) 108
AgsI TTSAA 3 cut(s) 126, 167, 193
AjnI CCWGG 1 cut(s) 35
AluBI AGCT 4 cut(s) 99, 181, 212, 316
AluI AGCT 4 cut(s) 99, 181, 212, 316
Alw21I GWGCWC 1 cut(s) 101
AlwI GGATC 1 cut(s) 149
AoxI GGCC 3 cut(s) 185, 249, 349
ApeKI GCWGC 2 cut(s) 313, 388
Asp718I GGTACC 1 cut(s) 338
AspLEI GCGC 1 cut(s) 86
AspS9I GGNCC 1 cut(s) 206
AsuC2I CCSGG 1 cut(s) 408
AsuHPI GGTGA 1 cut(s) 82
AvaII GGWCC 1 cut(s) 206
BaeI ACNNNNGTAYC 2 cut(s) 330, 363
BalI TGGCCA 1 cut(s) 187
BanI GGYRCC 1 cut(s) 338
BanII GRGCYC 1 cut(s) 101
Bbv12I GWGCWC 1 cut(s) 101
BbvI GCAGC 2 cut(s) 325, 400
BciT130I CCWGG 1 cut(s) 37
BclI TGATCA 1 cut(s) 282
BcnI CCSGG 1 cut(s) 408
BisI GCNGC 2 cut(s) 314, 389
BlsI GCNGC 2 cut(s) 315, 390
Bme1390I CCNGG 2 cut(s) 37, 408
Bme18I GGWCC 1 cut(s) 206
BmgT120I GGNCC 1 cut(s) 206
BmiI GGNNCC 1 cut(s) 340
BmrFI CCNGG 2 cut(s) 37, 408
BmsI GCATC 2 cut(s) 222, 322
Bpu10I CCTNAGC 1 cut(s) 95
BpuMI CCSGG 1 cut(s) 408
BsaJI CCNNGG 3 cut(s) 36, 246, 342
BsaXI ACNNNNNCTCC 2 cut(s) 136, 166
Bse1I ACTGG 1 cut(s) 33
BseBI CCWGG 1 cut(s) 37
BseDI CCNNGG 3 cut(s) 36, 246, 342
BseGI GGATG 2 cut(s) 66, 360
BseMII CTCAG 2 cut(s) 86, 204
BseNI ACTGG 1 cut(s) 33
BseXI GCAGC 2 cut(s) 325, 400
Bsh1236I CGCG 1 cut(s) 84
BshFI GGCC 3 cut(s) 187, 251, 351
BshNI GGYRCC 1 cut(s) 338
BsiHKAI GWGCWC 1 cut(s) 101
BsiSI CCGG 1 cut(s) 408
BslFI GGGAC 1 cut(s) 275
BsmFI GGGAC 1 cut(s) 275
BsmI GAATGC 1 cut(s) 280
BsnI GGCC 3 cut(s) 187, 251, 351
Bsp1286I GDGCHC 1 cut(s) 101
Bsp143I GATC 2 cut(s) 154, 282
BspACI CCGC 1 cut(s) 235
BspANI GGCC 3 cut(s) 187, 251, 351
BspCNI CTCAG 2 cut(s) 87, 205
BspFNI CGCG 1 cut(s) 84
BspLI GGNNCC 1 cut(s) 340
BspPI GGATC 1 cut(s) 149
BspT107I GGYRCC 1 cut(s) 338
BsrI ACTGG 1 cut(s) 33
BssECI CCNNGG 3 cut(s) 36, 246, 342
BssMI GATC 2 cut(s) 154, 282
Bst2UI CCWGG 1 cut(s) 37
Bst4CI ACNGT 3 cut(s) 268, 346, 384
BstDEI CTNAG 2 cut(s) 95, 213
BstDSI CCRYGG 1 cut(s) 342
BstF5I GGATG 2 cut(s) 66, 360
BstFNI CGCG 1 cut(s) 84
BstHHI GCGC 1 cut(s) 86
BstKTI GATC 2 cut(s) 157, 285
BstMBI GATC 2 cut(s) 154, 282
BstNI CCWGG 1 cut(s) 37
BstSCI CCNGG 2 cut(s) 35, 406
BstUI CGCG 1 cut(s) 84
BstV1I GCAGC 2 cut(s) 325, 400
BsuRI GGCC 3 cut(s) 187, 251, 351
BtgI CCRYGG 1 cut(s) 342
BtsCI GGATG 2 cut(s) 66, 360
BtsIMutI CAGTG 3 cut(s) 26, 198, 303
CfoI GCGC 1 cut(s) 86
Cfr13I GGNCC 1 cut(s) 206
Csp6I GTAC 1 cut(s) 339
CviAII CATG 2 cut(s) 16, 223
CviQI GTAC 1 cut(s) 339
DdeI CTNAG 2 cut(s) 95, 213
DpnI GATC 2 cut(s) 156, 284
DpnII GATC 2 cut(s) 154, 282
EaeI YGGCCR 1 cut(s) 185
Ecl136II GAGCTC 1 cut(s) 99
Eco147I AGGCCT 1 cut(s) 251
Eco24I GRGCYC 1 cut(s) 101
Eco47I GGWCC 1 cut(s) 206
Eco53kI GAGCTC 1 cut(s) 99
Eco57I CTGAAG 2 cut(s) 222, 302
EcoICRI GAGCTC 1 cut(s) 99
EcoRII CCWGG 1 cut(s) 35
EcoT38I GRGCYC 1 cut(s) 101
FaeI CATG 2 cut(s) 19, 226
FaiI YATR 6 cut(s) 17, 47, 80, 174, 224, 417
FaqI GGGAC 1 cut(s) 275
FatI CATG 2 cut(s) 15, 222
FauI CCCGC 1 cut(s) 228
FbaI TGATCA 1 cut(s) 282
Fnu4HI GCNGC 2 cut(s) 314, 389
FokI GGATG 2 cut(s) 73, 347
FriOI GRGCYC 1 cut(s) 101
Fsp4HI GCNGC 2 cut(s) 314, 389
GlaI GCGC 1 cut(s) 85
GluI GCNGC 2 cut(s) 314, 389
HaeIII GGCC 3 cut(s) 187, 251, 351
HapII CCGG 1 cut(s) 408
HhaI GCGC 1 cut(s) 86
Hin1II CATG 2 cut(s) 19, 226
Hin6I GCGC 1 cut(s) 84
HinP1I GCGC 1 cut(s) 84
HincII GTYRAC 1 cut(s) 106
HindII GTYRAC 1 cut(s) 106
HinfI GANTC 3 cut(s) 32, 122, 308
HpaI GTTAAC 1 cut(s) 106
HpaII CCGG 1 cut(s) 408
HphI GGTGA 1 cut(s) 82
Hpy166II GTNNAC 2 cut(s) 73, 106
Hpy188I TCNGA 1 cut(s) 121
Hpy188III TCNNGA 1 cut(s) 433
Hpy8I GTNNAC 2 cut(s) 73, 106
HpyAV CCTTC 3 cut(s) 135, 168, 197
HpyCH4III ACNGT 3 cut(s) 268, 346, 384
HpyCH4IV ACGT 2 cut(s) 108, 429
HpyF3I CTNAG 2 cut(s) 95, 213
HpySE526I ACGT 2 cut(s) 108, 429
Hsp92II CATG 2 cut(s) 19, 226
HspAI GCGC 1 cut(s) 84
KpnI GGTACC 1 cut(s) 342
Ksp22I TGATCA 1 cut(s) 282
KspAI GTTAAC 1 cut(s) 106
Kzo9I GATC 2 cut(s) 154, 282
LpnPI CCDG 9 cut(s) 14, 22, 49, 107, 222, 305, 306, 321, 421
Lsp1109I GCAGC 2 cut(s) 325, 400
LweI GCATC 2 cut(s) 222, 322
MaeII ACGT 2 cut(s) 108, 429
MaeIII GTNAC 1 cut(s) 196
MalI GATC 2 cut(s) 156, 284
MboI GATC 2 cut(s) 154, 282
MboII GAAGA 1 cut(s) 283
MhlI GDGCHC 1 cut(s) 101
MlsI TGGCCA 1 cut(s) 187
MluCI AATT 2 cut(s) 48, 169
MluNI TGGCCA 1 cut(s) 187
MlyI GAGTC 1 cut(s) 302
MnlI CCTC 2 cut(s) 51, 241
Mox20I TGGCCA 1 cut(s) 187
MscI TGGCCA 1 cut(s) 187
MseI TTAA 3 cut(s) 105, 134, 460
MslI CAYNNNNRTG 1 cut(s) 227
Msp20I TGGCCA 1 cut(s) 187
MspA1I CMGCKG 1 cut(s) 212
MspI CCGG 1 cut(s) 408
MspR9I CCNGG 2 cut(s) 37, 408
Mva1269I GAATGC 1 cut(s) 280
MvaI CCWGG 1 cut(s) 37
MvnI CGCG 1 cut(s) 84
NciI CCSGG 1 cut(s) 408
NdeII GATC 2 cut(s) 154, 282
NlaIII CATG 2 cut(s) 19, 226
NlaIV GGNNCC 1 cut(s) 340
NmeAIII GCCGAG 2 cut(s) 237, 271
NmuCI GTSAC 1 cut(s) 196
PceI AGGCCT 1 cut(s) 251
PctI GAATGC 1 cut(s) 280
PfeI GAWTC 2 cut(s) 32, 122
PkrI GCNGC 2 cut(s) 315, 390
PleI GAGTC 1 cut(s) 302
PpsI GAGTC 1 cut(s) 302
Psp124BI GAGCTC 1 cut(s) 101
Psp6I CCWGG 1 cut(s) 35
PspGI CCWGG 1 cut(s) 35
PspN4I GGNNCC 1 cut(s) 340
PspPI GGNCC 1 cut(s) 206
PvuII CAGCTG 1 cut(s) 212
RsaI GTAC 1 cut(s) 340
RsaNI GTAC 1 cut(s) 339
RseI CAYNNNNRTG 1 cut(s) 227
SacI GAGCTC 1 cut(s) 101
SaqAI TTAA 3 cut(s) 105, 134, 460
SatI GCNGC 2 cut(s) 314, 389
Sau3AI GATC 2 cut(s) 154, 282
Sau96I GGNCC 1 cut(s) 206
SchI GAGTC 1 cut(s) 302
ScrFI CCNGG 2 cut(s) 37, 408
SduI GDGCHC 1 cut(s) 101
SetI ASST 8 cut(s) 101, 111, 183, 198, 208, 214, 318, 432
SfaNI GCATC 2 cut(s) 222, 322
SinI GGWCC 1 cut(s) 206
SmiMI CAYNNNNRTG 1 cut(s) 227
Sse9I AATT 2 cut(s) 48, 169
SseBI AGGCCT 1 cut(s) 251
SsiI CCGC 1 cut(s) 235
SstI GAGCTC 1 cut(s) 101
StuI AGGCCT 1 cut(s) 251
StyD4I CCNGG 2 cut(s) 35, 406
TaaI ACNGT 3 cut(s) 268, 346, 384
TaiI ACGT 2 cut(s) 111, 432
TasI AATT 2 cut(s) 48, 169
TfiI GAWTC 2 cut(s) 32, 122
Tru1I TTAA 3 cut(s) 105, 134, 460
Tru9I TTAA 3 cut(s) 105, 134, 460
TscAI CASTG 3 cut(s) 33, 205, 310
TseFI GTSAC 1 cut(s) 196
TseI GCWGC 2 cut(s) 313, 388
Tsp45I GTSAC 1 cut(s) 196
TspDTI ATGAA 1 cut(s) 32
TspRI CASTG 3 cut(s) 33, 205, 310
VpaK11BI GGWCC 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.