MD03G1203500.v1.1
ERF Family

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin Fzo YdjA family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
N/A
Physical Location & Seq
Forward (+)
27822093 .. 27829107
7015 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1203500.v1.1.491

Sequence Viewer

Length: 339 bp
ATGACGATCATCTACGGGTTGGTGCTGATCGGCGTTGTTTTTGCGTATACTAGAAGTGCGGGCATCGTATTGCTCCTCGCTCTTGTTGGATATATCAGTCTCAAAGACTCCGAGAGGAAGAAGAACTCGTATCCTGCTTTGAATCTCGAAACGGTTCCGATCAGTTCGTGCTCGAAAGATCATCAGAAAATCTACCAGGAATGGTTTCGATTCGTTGATTCAGACGCTGATGGCCGCATCACCGGGAGTGATGCTATAAAGTTCTTCGGCATGGCCAATCTGAATCGGGAGGAGCTCAAGCAGACCGTTTGGAAAGTAGTCTGTTATCTTTGGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005886 GO:0005911 GO:0005929 GO:0006810 GO:0006886 GO:0006897 GO:0006950 GO:0006970 GO:0006972 GO:0006996 GO:0007275 GO:0007399 GO:0008104 GO:0008150 GO:0009506 GO:0009628 GO:0009651 GO:0009719 GO:0009987 GO:0010008 GO:0010033 GO:0010830 GO:0010831 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016192 GO:0016197 GO:0020016 GO:0020018 GO:0022008 GO:0022603 GO:0022607 GO:0030030 GO:0030031 GO:0030054 GO:0030154 GO:0030182 GO:0031090 GO:0031175 GO:0031253 GO:0031410 GO:0031901 GO:0031982 GO:0032386 GO:0032388 GO:0032456 GO:0032501 GO:0032502 GO:0032879 GO:0033036 GO:0033365 GO:0034613 GO:0042221 GO:0042538 GO:0042886 GO:0042995 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044440 GO:0044441 GO:0044444 GO:0044446 GO:0044459 GO:0044463 GO:0044464 GO:0044782 GO:0045184 GO:0045595 GO:0045597 GO:0046907 GO:0048468 GO:0048518 GO:0048522 GO:0048666 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051050 GO:0051094 GO:0051128 GO:0051130 GO:0051147 GO:0051149 GO:0051153 GO:0051155 GO:0051179 GO:0051234 GO:0051259 GO:0051260 GO:0051641 GO:0051649 GO:0051716 GO:0055037 GO:0055038 GO:0055044 GO:0060142 GO:0060143 GO:0060170 GO:0060271 GO:0060341 GO:0060627 GO:0061512 GO:0065003 GO:0065007 GO:0070727 GO:0070848 GO:0070887 GO:0070925 GO:0071310 GO:0071363 GO:0071495 GO:0071702 GO:0071705 GO:0071840 GO:0071944 GO:0097708 GO:0098588 GO:0098590 GO:0098657 GO:0098805 GO:0120025 GO:0120031 GO:0120036 GO:0120038 GO:1901739 GO:1901741 GO:1990089 GO:1990090 GO:2001135 GO:2001137
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Dynamin_N EF-hand_4 EHD_N

Protein Analysis

113

Amino Acids

12.76

Weight (kDa)

7.73

Isoelectric Point (pI)

25.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 47
AciI CCGC 2 cut(s) 59, 235
AcoI YGGCCR 2 cut(s) 232, 273
AgsI TTSAA 1 cut(s) 142
AjnI CCWGG 1 cut(s) 195
AluBI AGCT 1 cut(s) 295
AluI AGCT 1 cut(s) 295
Alw21I GWGCWC 2 cut(s) 173, 297
Alw26I GTCTC 1 cut(s) 104
AlwNI CAGNNNCTG 1 cut(s) 227
AoxI GGCC 2 cut(s) 232, 273
Asp700I GAANNNNTTC 2 cut(s) 153, 204
AsuC2I CCSGG 1 cut(s) 244
AsuHPI GGTGA 1 cut(s) 232
BalI TGGCCA 1 cut(s) 275
BanII GRGCYC 1 cut(s) 297
Bbv12I GWGCWC 2 cut(s) 173, 297
BccI CCATC 1 cut(s) 224
BciT130I CCWGG 1 cut(s) 197
BciVI GTATCC 1 cut(s) 141
BcnI CCSGG 1 cut(s) 244
BcoDI GTCTC 1 cut(s) 104
BfaI CTAG 1 cut(s) 51
BfuI GTATCC 1 cut(s) 141
BisI GCNGC 1 cut(s) 235
BlsI GCNGC 1 cut(s) 236
Bme1390I CCNGG 2 cut(s) 197, 244
BmiI GGNNCC 1 cut(s) 156
BmrFI CCNGG 2 cut(s) 197, 244
BmsI GCATC 3 cut(s) 72, 241, 246
BpuEI CTTGAG 1 cut(s) 281
BpuMI CCSGG 1 cut(s) 244
BseBI CCWGG 1 cut(s) 197
BseRI GAGGAG 2 cut(s) 65, 305
BshFI GGCC 2 cut(s) 234, 275
BsiHKAI GWGCWC 2 cut(s) 173, 297
BsiSI CCGG 1 cut(s) 243
BsmAI GTCTC 1 cut(s) 104
BsnI GGCC 2 cut(s) 234, 275
Bsp1286I GDGCHC 2 cut(s) 173, 297
Bsp143I GATC 4 cut(s) 6, 27, 159, 178
BspACI CCGC 2 cut(s) 59, 235
BspANI GGCC 2 cut(s) 234, 275
BspLI GGNNCC 1 cut(s) 156
BssMI GATC 4 cut(s) 6, 27, 159, 178
BssNAI GTATAC 1 cut(s) 48
Bst1107I GTATAC 1 cut(s) 48
Bst2UI CCWGG 1 cut(s) 197
Bst4CI ACNGT 2 cut(s) 154, 307
BstC8I GCNNGC 1 cut(s) 61
BstKTI GATC 4 cut(s) 9, 30, 162, 181
BstMAI GTCTC 1 cut(s) 104
BstMBI GATC 4 cut(s) 6, 27, 159, 178
BstNI CCWGG 1 cut(s) 197
BstSCI CCNGG 2 cut(s) 195, 242
BstZ17I GTATAC 1 cut(s) 48
BsuI GTATCC 1 cut(s) 141
BsuRI GGCC 2 cut(s) 234, 275
Cac8I GCNNGC 1 cut(s) 61
CaiI CAGNNNCTG 1 cut(s) 227
CseI GACGC 1 cut(s) 233
CviAII CATG 1 cut(s) 271
CviJI RGCY 3 cut(s) 234, 275, 295
CviKI_1 RGCY 3 cut(s) 234, 275, 295
DpnI GATC 4 cut(s) 8, 29, 161, 180
DpnII GATC 4 cut(s) 6, 27, 159, 178
EaeI YGGCCR 2 cut(s) 232, 273
Ecl136II GAGCTC 1 cut(s) 295
Eco24I GRGCYC 1 cut(s) 297
Eco53kI GAGCTC 1 cut(s) 295
EcoICRI GAGCTC 1 cut(s) 295
EcoRII CCWGG 1 cut(s) 195
EcoT38I GRGCYC 1 cut(s) 297
FaeI CATG 1 cut(s) 274
FaiI YATR 4 cut(s) 48, 93, 257, 272
FatI CATG 1 cut(s) 270
FauI CCCGC 1 cut(s) 52
FblI GTMKAC 1 cut(s) 47
Fnu4HI GCNGC 1 cut(s) 235
FriOI GRGCYC 1 cut(s) 297
Fsp4HI GCNGC 1 cut(s) 235
FspBI CTAG 1 cut(s) 51
GluI GCNGC 1 cut(s) 235
HaeIII GGCC 2 cut(s) 234, 275
HapII CCGG 1 cut(s) 243
HgaI GACGC 1 cut(s) 233
Hin1II CATG 1 cut(s) 274
HinfI GANTC 5 cut(s) 107, 142, 210, 218, 283
HpaII CCGG 1 cut(s) 243
HphI GGTGA 1 cut(s) 232
Hpy166II GTNNAC 1 cut(s) 48
Hpy188I TCNGA 5 cut(s) 112, 159, 186, 223, 282
Hpy188III TCNNGA 2 cut(s) 146, 287
Hpy8I GTNNAC 1 cut(s) 48
HpyCH4III ACNGT 2 cut(s) 154, 307
Hsp92II CATG 1 cut(s) 274
Kzo9I GATC 4 cut(s) 6, 27, 159, 178
LmnI GCTCC 2 cut(s) 78, 292
LpnPI CCDG 4 cut(s) 147, 182, 209, 256
LweI GCATC 3 cut(s) 72, 241, 246
MaeI CTAG 1 cut(s) 51
MalI GATC 4 cut(s) 8, 29, 161, 180
MboI GATC 4 cut(s) 6, 27, 159, 178
MboII GAAGA 3 cut(s) 130, 133, 256
MhlI GDGCHC 2 cut(s) 173, 297
MlsI TGGCCA 1 cut(s) 275
MluNI TGGCCA 1 cut(s) 275
MlyI GAGTC 1 cut(s) 101
MmeI TCCRAC 1 cut(s) 67
MnlI CCTC 3 cut(s) 86, 108, 283
Mox20I TGGCCA 1 cut(s) 275
MroXI GAANNNNTTC 2 cut(s) 153, 204
MscI TGGCCA 1 cut(s) 275
Msp20I TGGCCA 1 cut(s) 275
MspI CCGG 1 cut(s) 243
MspR9I CCNGG 2 cut(s) 197, 244
MvaI CCWGG 1 cut(s) 197
NciI CCSGG 1 cut(s) 244
NdeII GATC 4 cut(s) 6, 27, 159, 178
NlaIII CATG 1 cut(s) 274
NlaIV GGNNCC 1 cut(s) 156
PdmI GAANNNNTTC 2 cut(s) 153, 204
PfeI GAWTC 4 cut(s) 142, 210, 218, 283
PkrI GCNGC 1 cut(s) 236
PleI GAGTC 1 cut(s) 101
PpsI GAGTC 1 cut(s) 101
Psp124BI GAGCTC 1 cut(s) 297
Psp6I CCWGG 1 cut(s) 195
PspGI CCWGG 1 cut(s) 195
PspN4I GGNNCC 1 cut(s) 156
PstNI CAGNNNCTG 1 cut(s) 227
SacI GAGCTC 1 cut(s) 297
SatI GCNGC 1 cut(s) 235
Sau3AI GATC 4 cut(s) 6, 27, 159, 178
SchI GAGTC 1 cut(s) 101
ScrFI CCNGG 2 cut(s) 197, 244
SduI GDGCHC 2 cut(s) 173, 297
SetI ASST 1 cut(s) 297
SfaNI GCATC 3 cut(s) 72, 241, 246
SmlI CTYRAG 1 cut(s) 296
SmoI CTYRAG 1 cut(s) 296
SsiI CCGC 2 cut(s) 59, 235
SspMI CTAG 1 cut(s) 51
SstI GAGCTC 1 cut(s) 297
StyD4I CCNGG 2 cut(s) 195, 242
TaaI ACNGT 2 cut(s) 154, 307
TaqI TCGA 3 cut(s) 147, 173, 208
TauI GCSGC 1 cut(s) 237
TfiI GAWTC 4 cut(s) 142, 210, 218, 283
XmiI GTMKAC 1 cut(s) 47
XmnI GAANNNNTTC 2 cut(s) 153, 204
XspI CTAG 1 cut(s) 51
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.