Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

N/A

Physical Location & Seq

Genomic Coordinates Forward (+)

28779126 .. 28782698

Gene Structure 3573 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1201500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 942 bp

ATGCGAACGATCAAGGCGCCCTCCTGGGTCGCATTCACTGATACGGTGCGTCTGATCGGAGAGGCGGCCACGAATCAAGCCCCTCTCAACGCAGAACGCACCATTTCTGACGTCAAGCGGCTCATTGGGCGAAAGAGACTGATTTTATGTGTATGGGAAGAATTTAAGCAACTTTACATGGTACTTGGATTGGAAGATGTTGTGGATTATCTGTGTGTTCGTGGTGGGAATTTCCGTGGGGAATGCTTCCTGGCCGACGAGCACACAGCTCCCCGAGAGGCTGTGAACGAAGATGGGAAGCCTTACATGCATGTGAACGTTAAAGGTGAAACCAAGGTTTTTAGCCCTGAGGAAATAAGTGCTGTGATTTTGGGTAAGATGAAGGAAACCGCCGGAGGCCTACCTCGGGAAGAAAATCAAAGACGCCGTGCTTATTTCAATGATGCGCAGAGGCAGGTTACCAAGGATGCGGGCATCATTGCCGGGCTCAATGTGAGGTCTATGAGTGGAGACACTCACTTGGGAGGAGAGGACTTCGACCATGGAGTGATGGACTACGTCATCAAGTTCATCAAGAAGAAATATAACAAGGATATTAGCAAGGACCCAAGGCTCTCGGGAAACTTCGAAAGGAACATGGACTTGTTCAAGAAGATCATGAGACCGGTAAAGAAGGCTTTAGAAGATGCAGGCCTGAAGAAGAAAGATATTCACGAGATTGTCCTCGTTGGAGGGAGTACCCGAATTCCTAAGGTGCAAGAGATGTTGAAAGACTTCTTCGATGGGAAAGAGCCAAGCAAGGGGGTCAATCCTGATAAGGCTGTTGCATACAGTGCTGCAGTTCAAGGTGGAGTTCTCAGTGGCGAAGGCGGGGAAGAAACCAAGGGTAATTGGACTGTTTCAATTGACATTTTGTATAATATTTTGTTCCCGATATGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005783 GO:0005788 GO:0008150 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0012505 GO:0016043 GO:0016049 GO:0016592 GO:0022414 GO:0030154 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032989 GO:0032991 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044432 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0044706 GO:0048468 GO:0048588 GO:0048589 GO:0048856 GO:0048868 GO:0048869 GO:0051704 GO:0060560 GO:0070013 GO:0071840

KEGG Pathways

Metabolic & Signaling

ko03060 ko04141 ko04918 ko05020 map03060 map04141 map04918 map05020

Pfam Domains

Protein Families

HSP70

Protein Analysis

314

Amino Acids

34.97

Weight (kDa)

8.49

Isoelectric Point (pI)

34.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HSP70	PF00012	6 - 46	3.8e-09	Hsp70 protein
HSP70	PF00012	78 - 165	1.8e-14	Hsp70 protein
HSP70	PF00012	163 - 205	8.5e-09	Hsp70 protein
HSP70	PF00012	212 - 289	2.1e-31	Hsp70 protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	114
Acc16I	TGCGCA	1 cut(s)	447
Acc36I	ACCTGC	1 cut(s)	445
AccB1I	GGYRCC	1 cut(s)	16
AciI	CCGC	5 cut(s)	65, 118, 390, 470, 870
AclI	AACGTT	1 cut(s)	318
AcoI	YGGCCR	2 cut(s)	66, 252
AcsI	RAATTY	3 cut(s)	161, 229, 744
AcuI	CTGAAG	1 cut(s)	716
AcyI	GRCGYC	3 cut(s)	17, 111, 424
AfaI	GTAC	2 cut(s)	183, 739
AfiI	CCNNNNNNNGG	2 cut(s)	406, 800
AgeI	ACCGGT	1 cut(s)	664
AgsI	TTSAA	5 cut(s)	439, 649, 769, 845, 903
AjnI	CCWGG	2 cut(s)	23, 249
AluBI	AGCT	1 cut(s)	269
AluI	AGCT	1 cut(s)	269
Alw21I	GWGCWC	1 cut(s)	264
Alw26I	GTCTC	3 cut(s)	130, 504, 655
Ama87I	CYCGRG	3 cut(s)	273, 405, 616
AoxI	GGCC	4 cut(s)	66, 252, 397, 691
ApeKI	GCWGC	1 cut(s)	836
ApoI	RAATTY	3 cut(s)	161, 229, 744
AsiGI	ACCGGT	1 cut(s)	664
Asp700I	GAANNNNTTC	1 cut(s)	773
AspLEI	GCGC	2 cut(s)	19, 448
AspS9I	GGNCC	1 cut(s)	604
AsuC2I	CCSGG	1 cut(s)	484
AsuHPI	GGTGA	1 cut(s)	338
AsuII	TTCGAA	1 cut(s)	627
AvaI	CYCGRG	3 cut(s)	273, 405, 616
AvaII	GGWCC	1 cut(s)	604
AxyI	CCTNAGG	2 cut(s)	348, 750
BanI	GGYRCC	1 cut(s)	16
BanII	GRGCYC	1 cut(s)	489
BauI	CACGAG	1 cut(s)	713
Bbv12I	GWGCWC	1 cut(s)	264
BbvI	GCAGC	1 cut(s)	823
BccI	CCATC	3 cut(s)	287, 544, 776
BceAI	ACGGC	1 cut(s)	411
BciT130I	CCWGG	2 cut(s)	25, 251
BcnI	CCSGG	1 cut(s)	484
BcoDI	GTCTC	3 cut(s)	130, 504, 655
BfmI	CTRYAG	1 cut(s)	837
BfoI	RGCGCY	1 cut(s)	20
BfuAI	ACCTGC	1 cut(s)	445
BisI	GCNGC	3 cut(s)	66, 119, 837
BlsI	GCNGC	3 cut(s)	67, 120, 838
Bme1390I	CCNGG	3 cut(s)	25, 251, 484
Bme18I	GGWCC	1 cut(s)	604
BmeT110I	CYCGRG	3 cut(s)	273, 405, 616
BmgT120I	GGNCC	1 cut(s)	604
BmiI	GGNNCC	2 cut(s)	18, 606
BmrFI	CCNGG	3 cut(s)	25, 251, 484
BmsI	GCATC	4 cut(s)	433, 457, 483, 676
Bpu14I	TTCGAA	1 cut(s)	627
BpuMI	CCSGG	1 cut(s)	484
BsaHI	GRCGYC	3 cut(s)	17, 111, 424
BsaI	GGTCTC	1 cut(s)	655
BsaJI	CCNNGG	8 cut(s)	24, 235, 333, 404, 462, 541, 608, 882
BsaWI	WCCGGW	1 cut(s)	664
Bsc4I	CCNNNNNNNGG	2 cut(s)	406, 800
Bse118I	RCCGGY	1 cut(s)	664
Bse21I	CCTNAGG	2 cut(s)	348, 750
Bse3DI	GCAATG	1 cut(s)	477
BseBI	CCWGG	2 cut(s)	25, 251
BseDI	CCNNGG	8 cut(s)	24, 235, 333, 404, 462, 541, 608, 882
BseGI	GGATG	1 cut(s)	472
BseLI	CCNNNNNNNGG	2 cut(s)	406, 800
BseMI	GCAATG	1 cut(s)	477
BseMII	CTCAG	2 cut(s)	339, 871
BseRI	GAGGAG	1 cut(s)	540
BseXI	GCAGC	1 cut(s)	823
BshFI	GGCC	4 cut(s)	68, 254, 399, 693
BshNI	GGYRCC	1 cut(s)	16
BshTI	ACCGGT	1 cut(s)	664
BsiHKAI	GWGCWC	1 cut(s)	264
BsiHKCI	CYCGRG	3 cut(s)	273, 405, 616
BsiSI	CCGG	3 cut(s)	393, 483, 665
BslI	CCNNNNNNNGG	2 cut(s)	406, 800
BsmAI	GTCTC	3 cut(s)	130, 504, 655
BsmI	GAATGC	2 cut(s)	32, 248
BsnI	GGCC	4 cut(s)	68, 254, 399, 693
Bso31I	GGTCTC	1 cut(s)	655
BsoBI	CYCGRG	3 cut(s)	273, 405, 616
Bsp119I	TTCGAA	1 cut(s)	627
Bsp1286I	GDGCHC	2 cut(s)	264, 489
Bsp143I	GATC	3 cut(s)	9, 54, 654
Bsp19I	CCATGG	1 cut(s)	541
BspACI	CCGC	5 cut(s)	65, 118, 390, 470, 870
BspANI	GGCC	4 cut(s)	68, 254, 399, 693
BspCNI	CTCAG	2 cut(s)	340, 870
BspHI	TCATGA	1 cut(s)	657
BspLI	GGNNCC	2 cut(s)	18, 606
BspMAI	CTGCAG	1 cut(s)	841
BspMI	ACCTGC	1 cut(s)	445
BspT104I	TTCGAA	1 cut(s)	627
BspT107I	GGYRCC	1 cut(s)	16
BspTNI	GGTCTC	1 cut(s)	655
BsrDI	GCAATG	1 cut(s)	477
BsrFI	RCCGGY	1 cut(s)	664
BssAI	RCCGGY	1 cut(s)	664
BssECI	CCNNGG	8 cut(s)	24, 235, 333, 404, 462, 541, 608, 882
BssMI	GATC	3 cut(s)	9, 54, 654
BssNI	GRCGYC	3 cut(s)	17, 111, 424
BssSI	CACGAG	1 cut(s)	713
BssT1I	CCWWGG	5 cut(s)	333, 462, 541, 608, 882
Bst2BI	CACGAG	1 cut(s)	713
Bst2UI	CCWGG	2 cut(s)	25, 251
Bst4CI	ACNGT	3 cut(s)	46, 833, 898
BstACI	GRCGYC	3 cut(s)	17, 111, 424
BstAPI	GCANNNNNTGC	1 cut(s)	833
BstBI	TTCGAA	1 cut(s)	627
BstC8I	GCNNGC	2 cut(s)	472, 691
BstDEI	CTNAG	3 cut(s)	348, 750, 857
BstDSI	CCRYGG	2 cut(s)	235, 541
BstEII	GGTNACC	1 cut(s)	457
BstF5I	GGATG	1 cut(s)	472
BstH2I	RGCGCY	1 cut(s)	20
BstHHI	GCGC	2 cut(s)	19, 448
BstKTI	GATC	3 cut(s)	12, 57, 657
BstMAI	GTCTC	3 cut(s)	130, 504, 655
BstMBI	GATC	3 cut(s)	9, 54, 654
BstMWI	GCNNNNNNNGC	3 cut(s)	127, 307, 833
BstNI	CCWGG	2 cut(s)	25, 251
BstNSI	RCATGY	2 cut(s)	310, 314
BstPI	GGTNACC	1 cut(s)	457
BstSCI	CCNGG	3 cut(s)	23, 249, 482
BstSFI	CTRYAG	1 cut(s)	837
BstV1I	GCAGC	1 cut(s)	823
Bsu36I	CCTNAGG	2 cut(s)	348, 750
BsuRI	GGCC	4 cut(s)	68, 254, 399, 693
BtgI	CCRYGG	2 cut(s)	235, 541
BtsCI	GGATG	1 cut(s)	472
BtsIMutI	CAGTG	3 cut(s)	36, 838, 865
BveI	ACCTGC	1 cut(s)	445
Cac8I	GCNNGC	2 cut(s)	472, 691
CciI	TCATGA	1 cut(s)	657
CfoI	GCGC	2 cut(s)	19, 448
Cfr10I	RCCGGY	1 cut(s)	664
Cfr13I	GGNCC	1 cut(s)	604
CseI	GACGC	2 cut(s)	38, 432
Csp6I	GTAC	2 cut(s)	182, 738
CspAI	ACCGGT	1 cut(s)	664
CviAII	CATG	6 cut(s)	178, 307, 311, 542, 637, 658
CviQI	GTAC	2 cut(s)	182, 738
DdeI	CTNAG	3 cut(s)	348, 750, 857
DinI	GGCGCC	1 cut(s)	18
DpnI	GATC	3 cut(s)	11, 56, 656
DpnII	GATC	3 cut(s)	9, 54, 654
EaeI	YGGCCR	2 cut(s)	66, 252
Eco130I	CCWWGG	5 cut(s)	333, 462, 541, 608, 882
Eco147I	AGGCCT	2 cut(s)	399, 693
Eco24I	GRGCYC	1 cut(s)	489
Eco31I	GGTCTC	1 cut(s)	655
Eco47I	GGWCC	1 cut(s)	604
Eco57I	CTGAAG	1 cut(s)	716
Eco81I	CCTNAGG	2 cut(s)	348, 750
Eco88I	CYCGRG	3 cut(s)	273, 405, 616
Eco91I	GGTNACC	1 cut(s)	457
EcoO109I	RGGNCCY	1 cut(s)	604
EcoO65I	GGTNACC	1 cut(s)	457
EcoRI	GAATTC	1 cut(s)	744
EcoRII	CCWGG	2 cut(s)	23, 249
EcoT14I	CCWWGG	5 cut(s)	333, 462, 541, 608, 882
EcoT22I	ATGCAT	1 cut(s)	312
EcoT38I	GRGCYC	1 cut(s)	489
EgeI	GGCGCC	1 cut(s)	18
EheI	GGCGCC	1 cut(s)	18
ErhI	CCWWGG	5 cut(s)	333, 462, 541, 608, 882
FaeI	CATG	6 cut(s)	181, 310, 314, 545, 640, 661
FatI	CATG	6 cut(s)	177, 306, 310, 541, 636, 657
FauI	CCCGC	2 cut(s)	463, 863
Fnu4HI	GCNGC	3 cut(s)	66, 119, 837
FokI	GGATG	1 cut(s)	479
FriOI	GRGCYC	1 cut(s)	489
Fsp4HI	GCNGC	3 cut(s)	66, 119, 837
FspI	TGCGCA	1 cut(s)	447
GlaI	GCGC	2 cut(s)	18, 447
GluI	GCNGC	3 cut(s)	66, 119, 837
HaeII	RGCGCY	1 cut(s)	20
HaeIII	GGCC	4 cut(s)	68, 254, 399, 693
HapII	CCGG	3 cut(s)	393, 483, 665
HgaI	GACGC	2 cut(s)	38, 432
HhaI	GCGC	2 cut(s)	19, 448
Hin1I	GRCGYC	3 cut(s)	17, 111, 424
Hin1II	CATG	6 cut(s)	181, 310, 314, 545, 640, 661
Hin6I	GCGC	2 cut(s)	17, 446
HinP1I	GCGC	2 cut(s)	17, 446
HinfI	GANTC	1 cut(s)	73
HpaII	CCGG	3 cut(s)	393, 483, 665
HphI	GGTGA	1 cut(s)	338
Hpy166II	GTNNAC	2 cut(s)	286, 316
Hpy188I	TCNGA	3 cut(s)	54, 59, 109
Hpy188III	TCNNGA	8 cut(s)	407, 574, 618, 649, 658, 713, 812, 931
Hpy8I	GTNNAC	2 cut(s)	286, 316
Hpy99I	CGWCG	1 cut(s)	260
HpyAV	CCTTC	3 cut(s)	376, 667, 860
HpyCH4III	ACNGT	3 cut(s)	46, 833, 898
HpyCH4IV	ACGT	3 cut(s)	111, 318, 558
HpyCH4V	TGCA	5 cut(s)	310, 689, 757, 827, 839
HpyF10VI	GCNNNNNNNGC	3 cut(s)	127, 307, 833
HpyF3I	CTNAG	3 cut(s)	348, 750, 857
HpySE526I	ACGT	3 cut(s)	111, 318, 558
Hsp92I	GRCGYC	3 cut(s)	17, 111, 424
Hsp92II	CATG	6 cut(s)	181, 310, 314, 545, 640, 661
HspAI	GCGC	2 cut(s)	17, 446
KasI	GGCGCC	1 cut(s)	16
Kzo9I	GATC	3 cut(s)	9, 54, 654
LmnI	GCTCC	1 cut(s)	274
Lsp1109I	GCAGC	1 cut(s)	823
LweI	GCATC	4 cut(s)	433, 457, 483, 676
MaeII	ACGT	3 cut(s)	111, 318, 558
MaeIII	GTNAC	1 cut(s)	457
MalI	GATC	3 cut(s)	11, 56, 656
MboI	GATC	3 cut(s)	9, 54, 654
MfeI	CAATTG	1 cut(s)	903
MhlI	GDGCHC	2 cut(s)	264, 489
MluCI	AATT	5 cut(s)	161, 229, 744, 889, 903
Mly113I	GGCGCC	1 cut(s)	17
MmeI	TCCRAC	1 cut(s)	709
Mph1103I	ATGCAT	1 cut(s)	312
MroXI	GAANNNNTTC	1 cut(s)	773
MseI	TTAA	3 cut(s)	165, 321, 940
MslI	CAYNNNNRTG	1 cut(s)	311
MspI	CCGG	3 cut(s)	393, 483, 665
MspR9I	CCNGG	3 cut(s)	25, 251, 484
MunI	CAATTG	1 cut(s)	903
Mva1269I	GAATGC	2 cut(s)	32, 248
MvaI	CCWGG	2 cut(s)	25, 251
MwoI	GCNNNNNNNGC	3 cut(s)	127, 307, 833
NarI	GGCGCC	1 cut(s)	17
NciI	CCSGG	1 cut(s)	484
NcoI	CCATGG	1 cut(s)	541
NdeII	GATC	3 cut(s)	9, 54, 654
NlaIII	CATG	6 cut(s)	181, 310, 314, 545, 640, 661
NlaIV	GGNNCC	2 cut(s)	18, 606
NsbI	TGCGCA	1 cut(s)	447
NsiI	ATGCAT	1 cut(s)	312
NspI	RCATGY	2 cut(s)	310, 314
NspV	TTCGAA	1 cut(s)	627
PagI	TCATGA	1 cut(s)	657
PceI	AGGCCT	2 cut(s)	399, 693
PctI	GAATGC	2 cut(s)	32, 248
PdmI	GAANNNNTTC	1 cut(s)	773
PfeI	GAWTC	1 cut(s)	73
PflFI	GACNNNGTC	1 cut(s)	557
PinAI	ACCGGT	1 cut(s)	664
PkrI	GCNGC	3 cut(s)	67, 120, 838
PluTI	GGCGCC	1 cut(s)	20
PpuMI	RGGWCCY	1 cut(s)	604
Psp1406I	AACGTT	1 cut(s)	318
Psp5II	RGGWCCY	1 cut(s)	604
Psp6I	CCWGG	2 cut(s)	23, 249
PspEI	GGTNACC	1 cut(s)	457
PspGI	CCWGG	2 cut(s)	23, 249
PspN4I	GGNNCC	2 cut(s)	18, 606
PspPI	GGNCC	1 cut(s)	604
PspPPI	RGGWCCY	1 cut(s)	604
PstI	CTGCAG	1 cut(s)	841
PsyI	GACNNNGTC	1 cut(s)	557
RsaI	GTAC	2 cut(s)	183, 739
RsaNI	GTAC	2 cut(s)	182, 738
RseI	CAYNNNNRTG	1 cut(s)	311
SaqAI	TTAA	3 cut(s)	165, 321, 940
SatI	GCNGC	3 cut(s)	66, 119, 837
Sau3AI	GATC	3 cut(s)	9, 54, 654
Sau96I	GGNCC	1 cut(s)	604
ScrFI	CCNGG	3 cut(s)	25, 251, 484
SduI	GDGCHC	2 cut(s)	264, 489
SfaNI	GCATC	4 cut(s)	433, 457, 483, 676
SfcI	CTRYAG	1 cut(s)	837
SfoI	GGCGCC	1 cut(s)	18
SfuI	TTCGAA	1 cut(s)	627
SinI	GGWCC	1 cut(s)	604
SmiMI	CAYNNNNRTG	1 cut(s)	311
Sse9I	AATT	5 cut(s)	161, 229, 744, 889, 903
SseBI	AGGCCT	2 cut(s)	399, 693
SsiI	CCGC	5 cut(s)	65, 118, 390, 470, 870
SspDI	GGCGCC	1 cut(s)	16
SspI	AATATT	1 cut(s)	922
StuI	AGGCCT	2 cut(s)	399, 693
StyD4I	CCNGG	3 cut(s)	23, 249, 482
StyI	CCWWGG	5 cut(s)	333, 462, 541, 608, 882
TaaI	ACNGT	3 cut(s)	46, 833, 898
TaiI	ACGT	3 cut(s)	114, 321, 561
TaqI	TCGA	3 cut(s)	537, 627, 780
TasI	AATT	5 cut(s)	161, 229, 744, 889, 903
TauI	GCSGC	2 cut(s)	68, 121
TfiI	GAWTC	1 cut(s)	73
Tru1I	TTAA	3 cut(s)	165, 321, 940
Tru9I	TTAA	3 cut(s)	165, 321, 940
TscAI	CASTG	3 cut(s)	43, 838, 865
TseI	GCWGC	1 cut(s)	836
TspDTI	ATGAA	2 cut(s)	395, 559
TspGWI	ACGGA	1 cut(s)	224
TspRI	CASTG	3 cut(s)	43, 838, 865
Tth111I	GACNNNGTC	1 cut(s)	557
VpaK11BI	GGWCC	1 cut(s)	604
XapI	RAATTY	3 cut(s)	161, 229, 744
XceI	RCATGY	2 cut(s)	310, 314
XmnI	GAANNNNTTC	1 cut(s)	773
ZraI	GACGTC	1 cut(s)	112
Zsp2I	ATGCAT	1 cut(s)	312

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.