MD07G1196600.v1.1
HSP70 Family

Belongs to the heat shock protein 70 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
N/A
Physical Location & Seq
Reverse (-)
27483018 .. 27486955
3938 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1196600.v1.1.491

Sequence Viewer

Length: 861 bp
ATGGCGGGAAAGGGAGAGGGGCCAGCGATCGGTATAGATCTCGGAACTACTTACTCGTGCGTCGGTGTTTGGCAACACGACCGCGTCGAGATCATCGTTAATGATCAGAGTAACCGCACGACGCCGTCTTACGTGGCGTTTACTGATACGGAGATGCTGATCGGCGATGCGGCCAAGAACCAGGTCGCCGTGAACCCCGTCAACACCGTCCTGGGTAAGAATCGAGATACTTTCTTTTTCTTGACGGAAACAAGCTTGCTGAGGCTGACGAGTTTGAGGAAAAGAAGAAGGAGCTGGAGAGCATCTGCAATTCGATCAGTGCCGAGATGTACCAGGGAACAGGGTGCTGGGGGAACTTTCCTCCTGCTGCTGGCAGCGGTGCTGGTCCCAATATTATTATCGAAGAAGTTGAGTAAGCGGTTTTTGTTGATCCAAAGTGTTTGCTTTGAAGAAAGTGGTGAACGTATTTTTCTGTTTCTTAGTTTAAGATGTAGGATGTTTGGTGATTTTGGTCATTTTGTTCAATATCGTATTCCGTGCAGCCTGACCCAAACACGTTTGGATTCAAAGCCCTTCGAAAGCCCAAAAGTCAAGTCACAGCCCACACGCAAAACGCCACCGTCCTGTGAAACGTTTTCCAACAAGGAAATTGACGAGAAGACAAATTTCCGACGACGAAGGGAGTGGACACGAAGTTTCGATCAAAAAGTAAAGAAACCAACCCCGCGAAGCAAATCAAATCATCACCGTCCACGACACTCGAATCCAACGGCCGTTATCAAACCGAACATTCTGGACACCCTCCCACCAAAAATCGTCAATATCCTCACGAACATTCTAGACAAGTGTCCTACTATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP70

Protein Analysis

287

Amino Acids

32.49

Weight (kDa)

10.19

Isoelectric Point (pI)

50.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 9 - 72 3.2e-25 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 84, 727
AciI CCGC 7 cut(s) 5, 82, 115, 170, 377, 418, 725
AclI AACGTT 1 cut(s) 632
AclWI GGATC 1 cut(s) 424
AcoI YGGCCR 2 cut(s) 171, 771
AcsI RAATTY 1 cut(s) 664
AcyI GRCGYC 1 cut(s) 122
AfaI GTAC 1 cut(s) 331
AfiI CCNNNNNNNGG 2 cut(s) 29, 370
AflIII ACRYGT 1 cut(s) 554
AgsI TTSAA 3 cut(s) 449, 524, 567
AjnI CCWGG 3 cut(s) 180, 210, 332
AluBI AGCT 2 cut(s) 255, 294
AluI AGCT 2 cut(s) 255, 294
AlwI GGATC 1 cut(s) 424
AoxI GGCC 3 cut(s) 20, 171, 771
ApeKI GCWGC 3 cut(s) 367, 374, 540
ApoI RAATTY 1 cut(s) 664
ArsI GACNNNNNNTTYG 2 cut(s) 578, 610
AspS9I GGNCC 2 cut(s) 20, 385
AsuHPI GGTGA 3 cut(s) 470, 515, 737
AsuII TTCGAA 1 cut(s) 576
AvaII GGWCC 1 cut(s) 385
BauI CACGAG 1 cut(s) 55
BbsI GAAGAC 1 cut(s) 665
BbvCI CCTCAGC 1 cut(s) 260
BbvI GCAGC 3 cut(s) 354, 386, 552
BceAI ACGGC 4 cut(s) 109, 173, 758, 786
BciT130I CCWGG 3 cut(s) 182, 212, 334
BclI TGATCA 1 cut(s) 103
BfaI CTAG 1 cut(s) 839
BglII AGATCT 1 cut(s) 37
BisI GCNGC 4 cut(s) 171, 368, 375, 541
BlsI GCNGC 4 cut(s) 172, 369, 376, 542
Bme1390I CCNGG 3 cut(s) 182, 212, 334
Bme18I GGWCC 1 cut(s) 385
BmgT120I GGNCC 2 cut(s) 20, 385
BmiI GGNNCC 2 cut(s) 21, 387
BmrFI CCNGG 3 cut(s) 182, 212, 334
BmsI GCATC 3 cut(s) 144, 157, 311
BoxI GACNNNNGTC 1 cut(s) 846
BpiI GAAGAC 1 cut(s) 665
BpmI CTGGAG 1 cut(s) 316
Bpu10I CCTNAGC 1 cut(s) 260
Bpu14I TTCGAA 1 cut(s) 576
BsaAI YACGTR 1 cut(s) 133
BsaBI GATNNNNATC 1 cut(s) 158
BsaHI GRCGYC 1 cut(s) 122
BsaJI CCNNGG 2 cut(s) 211, 333
Bsc4I CCNNNNNNNGG 2 cut(s) 29, 370
Bse8I GATNNNNATC 1 cut(s) 158
BseBI CCWGG 3 cut(s) 182, 212, 334
BseDI CCNNGG 2 cut(s) 211, 333
BseGI GGATG 1 cut(s) 501
BseJI GATNNNNATC 1 cut(s) 158
BseLI CCNNNNNNNGG 2 cut(s) 29, 370
BseMII CTCAG 1 cut(s) 251
BseX3I CGGCCG 1 cut(s) 771
BseXI GCAGC 3 cut(s) 354, 386, 552
BseYI CCCAGC 1 cut(s) 347
BsgI GTGCAG 1 cut(s) 559
Bsh1236I CGCG 2 cut(s) 84, 727
Bsh1285I CGRYCG 3 cut(s) 30, 82, 774
BshFI GGCC 3 cut(s) 22, 173, 773
BsiEI CGRYCG 3 cut(s) 30, 82, 774
BslFI GGGAC 1 cut(s) 371
BslI CCNNNNNNNGG 2 cut(s) 29, 370
BsmFI GGGAC 1 cut(s) 371
BsnI GGCC 3 cut(s) 22, 173, 773
Bsp119I TTCGAA 1 cut(s) 576
Bsp143I GATC 8 cut(s) 27, 37, 90, 103, 159, 314, 429, 700
BspACI CCGC 7 cut(s) 5, 82, 115, 170, 377, 418, 725
BspANI GGCC 3 cut(s) 22, 173, 773
BspCNI CTCAG 1 cut(s) 252
BspFNI CGCG 2 cut(s) 84, 727
BspLI GGNNCC 2 cut(s) 21, 387
BspPI GGATC 1 cut(s) 424
BspT104I TTCGAA 1 cut(s) 576
BssECI CCNNGG 2 cut(s) 211, 333
BssMI GATC 8 cut(s) 27, 37, 90, 103, 159, 314, 429, 700
BssNI GRCGYC 1 cut(s) 122
BssSI CACGAG 1 cut(s) 55
Bst2BI CACGAG 1 cut(s) 55
Bst2UI CCWGG 3 cut(s) 182, 212, 334
Bst4CI ACNGT 3 cut(s) 208, 621, 749
BstACI GRCGYC 1 cut(s) 122
BstBAI YACGTR 1 cut(s) 133
BstBI TTCGAA 1 cut(s) 576
BstC8I GCNNGC 3 cut(s) 24, 257, 372
BstDEI CTNAG 2 cut(s) 260, 479
BstF5I GGATG 1 cut(s) 501
BstFNI CGCG 2 cut(s) 84, 727
BstKTI GATC 8 cut(s) 30, 40, 93, 106, 162, 317, 432, 703
BstMBI GATC 8 cut(s) 27, 37, 90, 103, 159, 314, 429, 700
BstMCI CGRYCG 3 cut(s) 30, 82, 774
BstNI CCWGG 3 cut(s) 182, 212, 334
BstPAI GACNNNNGTC 1 cut(s) 846
BstSCI CCNGG 3 cut(s) 180, 210, 332
BstUI CGCG 2 cut(s) 84, 727
BstV1I GCAGC 3 cut(s) 354, 386, 552
BstV2I GAAGAC 1 cut(s) 665
BstX2I RGATCY 1 cut(s) 37
BstYI RGATCY 1 cut(s) 37
BstZI CGGCCG 1 cut(s) 771
BsuRI GGCC 3 cut(s) 22, 173, 773
BtgZI GCGATG 1 cut(s) 180
BtsCI GGATG 1 cut(s) 501
BtsIMutI CAGTG 1 cut(s) 324
Cac8I GCNNGC 3 cut(s) 24, 257, 372
Cfr13I GGNCC 2 cut(s) 20, 385
CseI GACGC 3 cut(s) 49, 73, 130
CsiI ACCWGGT 1 cut(s) 180
Csp6I GTAC 1 cut(s) 330
CviQI GTAC 1 cut(s) 330
DdeI CTNAG 2 cut(s) 260, 479
DpnI GATC 8 cut(s) 29, 39, 92, 105, 161, 316, 431, 702
DpnII GATC 8 cut(s) 27, 37, 90, 103, 159, 314, 429, 700
EaeI YGGCCR 2 cut(s) 171, 771
EagI CGGCCG 1 cut(s) 771
EclXI CGGCCG 1 cut(s) 771
Eco47I GGWCC 1 cut(s) 385
Eco52I CGGCCG 1 cut(s) 771
EcoRII CCWGG 3 cut(s) 180, 210, 332
FaiI YATR 3 cut(s) 35, 857, 859
FaqI GGGAC 1 cut(s) 371
FauI CCCGC 1 cut(s) 732
FbaI TGATCA 1 cut(s) 103
Fnu4HI GCNGC 4 cut(s) 171, 368, 375, 541
FokI GGATG 1 cut(s) 508
Fsp4HI GCNGC 4 cut(s) 171, 368, 375, 541
FspBI CTAG 1 cut(s) 839
GluI GCNGC 4 cut(s) 171, 368, 375, 541
GsaI CCCAGC 1 cut(s) 351
GsuI CTGGAG 1 cut(s) 316
HaeIII GGCC 3 cut(s) 22, 173, 773
HgaI GACGC 3 cut(s) 49, 73, 130
Hin1I GRCGYC 1 cut(s) 122
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HindIII AAGCTT 1 cut(s) 253
HinfI GANTC 3 cut(s) 220, 563, 763
HphI GGTGA 3 cut(s) 470, 515, 737
Hpy166II GTNNAC 6 cut(s) 141, 193, 202, 461, 687, 752
Hpy188I TCNGA 3 cut(s) 44, 108, 671
Hpy188III TCNNGA 6 cut(s) 88, 224, 241, 794, 829, 839
Hpy8I GTNNAC 6 cut(s) 141, 193, 202, 461, 687, 752
Hpy99I CGWCG 5 cut(s) 65, 89, 124, 675, 678
HpyAV CCTTC 3 cut(s) 282, 583, 672
HpyCH4III ACNGT 3 cut(s) 208, 621, 749
HpyCH4IV ACGT 4 cut(s) 132, 463, 556, 632
HpyCH4V TGCA 2 cut(s) 308, 540
HpyF3I CTNAG 2 cut(s) 260, 479
HpySE526I ACGT 4 cut(s) 132, 463, 556, 632
Hsp92I GRCGYC 1 cut(s) 122
Ksp22I TGATCA 1 cut(s) 103
Kzo9I GATC 8 cut(s) 27, 37, 90, 103, 159, 314, 429, 700
LmnI GCTCC 1 cut(s) 291
Lsp1109I GCAGC 3 cut(s) 354, 386, 552
LweI GCATC 3 cut(s) 144, 157, 311
MabI ACCWGGT 1 cut(s) 180
MaeI CTAG 1 cut(s) 839
MaeII ACGT 4 cut(s) 132, 463, 556, 632
MaeIII GTNAC 2 cut(s) 110, 594
MalI GATC 8 cut(s) 29, 39, 92, 105, 161, 316, 431, 702
MboI GATC 8 cut(s) 27, 37, 90, 103, 159, 314, 429, 700
MboII GAAGA 4 cut(s) 297, 415, 461, 670
MflI RGATCY 1 cut(s) 37
MluCI AATT 3 cut(s) 309, 648, 664
MmeI TCCRAC 3 cut(s) 663, 694, 791
MnlI CCTC 6 cut(s) 10, 255, 270, 371, 812, 836
MseI TTAA 2 cut(s) 99, 485
MspA1I CMGCKG 1 cut(s) 377
MspR9I CCNGG 3 cut(s) 182, 212, 334
MvaI CCWGG 3 cut(s) 182, 212, 334
MvnI CGCG 2 cut(s) 84, 727
NdeII GATC 8 cut(s) 27, 37, 90, 103, 159, 314, 429, 700
NlaIV GGNNCC 2 cut(s) 21, 387
NmeAIII GCCGAG 1 cut(s) 348
NmuCI GTSAC 1 cut(s) 594
NspV TTCGAA 1 cut(s) 576
PcsI WCGNNNNNNNCGW 2 cut(s) 84, 93
PfeI GAWTC 3 cut(s) 220, 563, 763
PflFI GACNNNGTC 2 cut(s) 83, 124
PkrI GCNGC 4 cut(s) 172, 369, 376, 542
Ple19I CGATCG 1 cut(s) 30
Ppu21I YACGTR 1 cut(s) 133
PshAI GACNNNNGTC 1 cut(s) 846
Psp1406I AACGTT 1 cut(s) 632
Psp6I CCWGG 3 cut(s) 180, 210, 332
PspFI CCCAGC 1 cut(s) 347
PspGI CCWGG 3 cut(s) 180, 210, 332
PspN4I GGNNCC 2 cut(s) 21, 387
PspPI GGNCC 2 cut(s) 20, 385
PsuI RGATCY 1 cut(s) 37
PsyI GACNNNGTC 2 cut(s) 83, 124
PvuI CGATCG 1 cut(s) 30
RsaI GTAC 1 cut(s) 331
RsaNI GTAC 1 cut(s) 330
SaqAI TTAA 2 cut(s) 99, 485
SatI GCNGC 4 cut(s) 171, 368, 375, 541
Sau3AI GATC 8 cut(s) 27, 37, 90, 103, 159, 314, 429, 700
Sau96I GGNCC 2 cut(s) 20, 385
ScrFI CCNGG 3 cut(s) 182, 212, 334
SetI ASST 7 cut(s) 135, 186, 257, 296, 466, 559, 635
SexAI ACCWGGT 1 cut(s) 180
SfaNI GCATC 3 cut(s) 144, 157, 311
SfuI TTCGAA 1 cut(s) 576
SinI GGWCC 1 cut(s) 385
Sse9I AATT 3 cut(s) 309, 648, 664
SsiI CCGC 7 cut(s) 5, 82, 115, 170, 377, 418, 725
SspI AATATT 1 cut(s) 393
SspMI CTAG 1 cut(s) 839
StyD4I CCNGG 3 cut(s) 180, 210, 332
TaaI ACNGT 3 cut(s) 208, 621, 749
TaiI ACGT 4 cut(s) 135, 466, 559, 635
TaqI TCGA 7 cut(s) 87, 223, 313, 401, 576, 699, 761
TasI AATT 3 cut(s) 309, 648, 664
TauI GCSGC 1 cut(s) 173
TfiI GAWTC 3 cut(s) 220, 563, 763
Tru1I TTAA 2 cut(s) 99, 485
Tru9I TTAA 2 cut(s) 99, 485
TscAI CASTG 1 cut(s) 324
TseFI GTSAC 1 cut(s) 594
TseI GCWGC 3 cut(s) 367, 374, 540
Tsp45I GTSAC 1 cut(s) 594
TspGWI ACGGA 3 cut(s) 164, 260, 525
TspRI CASTG 1 cut(s) 324
Tth111I GACNNNGTC 2 cut(s) 83, 124
VpaK11BI GGWCC 1 cut(s) 385
XapI RAATTY 1 cut(s) 664
XbaI TCTAGA 1 cut(s) 838
XspI CTAG 1 cut(s) 839
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.