MD07G1197200.v1.1
HSP70 Family

Heat shock cognate 70 kDa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
N/A
Physical Location & Seq
Reverse (-)
27535072 .. 27536967
1896 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1197200.v1.1.491

Sequence Viewer

Length: 1200 bp
ATGGAGGGAAAAGGAGAAGGGCCAGCGATCGGTATAGATCTCGGAACTACTTACTCGTGCGTCGGTGTTTGTCAACACGACCGCGTCGAGATCATCGCTAATGATCATGGCAACCACACGACGCCGTCTTACGTGGCGTTTACTGATACGGAGAGGTTGATCGGCAATGTGGCCAAGGACCAGGTCGCCATGAACCCCGTCAACACCGTCCTGGATAAGAATCGAGATACTTTCTTTTTCTTGCGAAATTTTTTTATGAGATTCTTTGACCCTTCTGTTCAGAGTGACATGAAGCACTGGCCCTTCAAGGTCATTTCCGGCTCCGGTGACAAGCCCACGATTGTTGTCACATACAAGGGCGAAGAGAAGCAGTTCACTGCTGAAGAAATTTCCTCCATGGTTCTCGCCACAAAGGATGCTGGGGTCATTGACGGCCTTAATGTTCCGTGCATTATGAATGAGCCCACCGCTGCCGCTTTTGCATACGATCTTGACAAGAAGGTCACCAGCGTTGGCGAGAAGAATGTCTTGATTTTCGATCTTGGCGGAGGCACTTTTGATGTCTCACTGCTCACAATTGAAAAAAGGGTCTTTGAAGTGAAGGCAACCGCCGGAGACACCCACTTTGGAGGAGAAGATTTCAACAACAGAATGATGAATCACTTTGTTCAAGAGGTCAAGAGGAAGCACAAGAAGGTTATCAGCAGCAGCCCAAGTGCTCGTAGGCGGTTGAGAATTGCTTGTGAGAGGGCAAAAAGGACTCTCTCCTCCACTGCTCAGACCACAATTGAGATTGATTCTTTGTACGAGGGTGTTGATTTCTACTCAACCATTACCCGTCCTAGGTTTGAAGAGCTTAACATGGATCTTTTCAGAAAGTGTATGGGGCCGGTTGAGAAGTGTTTGAGAGATACAAAGATGGACAAGAGCTCGGTCCATGATGTTTTGCTAGTGGGCGGTTCTATTAGGATTCTGAAGGTGCAGCAGTTGTTGCAGGACTTCTTCTATGGCAAAGAGCTATGCAAGAGCTGTTGGAAGCTAAACAGCTCCTGGAGTCGAGGGATCGAAGGCAGCAAGGAGCTCCCGAGCATGGCTGTTATCTGCATTGAAGAAGAAAGAATAAATGAAGGGTTAAAACCTCCAAAGGTTATTTTTTTGTTTAAATGTATGTGTGAGCGACAAGTGTACACTCCAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko04010 ko04141 ko04144 ko04213 ko04612 ko04915 ko05134 ko05145 ko05162 ko05164 ko05169 map03040 map04010 map04141 map04144 map04213 map04612 map04915 map05134 map05145 map05162 map05164 map05169
Pfam Domains
Protein Families
HSP70

Protein Analysis

400

Amino Acids

44.9

Weight (kDa)

6.73

Isoelectric Point (pI)

41.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 9 - 136 1.5e-36 Hsp70 protein
HSP70 PF00012 136 - 343 1.2e-73 Hsp70 protein
MreB_Mbl PF06723 143 - 333 3.2e-06 MreB/Mbl protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 500
AccII CGCG 1 cut(s) 84
AciI CCGC 7 cut(s) 82, 468, 474, 546, 609, 727, 957
AclWI GGATC 2 cut(s) 873, 1070
AcoI YGGCCR 1 cut(s) 171
AcsI RAATTY 2 cut(s) 247, 387
AcuI CTGAAG 2 cut(s) 402, 995
AcyI GRCGYC 1 cut(s) 122
AfaI GTAC 2 cut(s) 806, 1187
AfiI CCNNNNNNNGG 3 cut(s) 29, 843, 1090
AgsI TTSAA 7 cut(s) 307, 581, 596, 643, 671, 851, 1109
AjnI CCWGG 3 cut(s) 180, 210, 1049
AluBI AGCT 7 cut(s) 856, 930, 1018, 1029, 1039, 1047, 1081
AluI AGCT 7 cut(s) 856, 930, 1018, 1029, 1039, 1047, 1081
Alw21I GWGCWC 3 cut(s) 721, 932, 1083
Alw26I GTCTC 2 cut(s) 568, 609
AlwI GGATC 2 cut(s) 873, 1070
AlwNI CAGNNNCTG 1 cut(s) 1050
Ama87I CYCGRG 1 cut(s) 1084
AoxI GGCC 5 cut(s) 20, 171, 299, 433, 887
ApeKI GCWGC 5 cut(s) 470, 705, 708, 982, 1071
ApoI RAATTY 2 cut(s) 247, 387
ArsI GACNNNNNNTTYG 2 cut(s) 608, 640
Asp700I GAANNNNTTC 1 cut(s) 371
AspA2I CCTAGG 1 cut(s) 842
AspS9I GGNCC 5 cut(s) 20, 178, 300, 887, 934
AsuHPI GGTGA 2 cut(s) 338, 496
AvaI CYCGRG 1 cut(s) 1084
AvaII GGWCC 2 cut(s) 178, 934
AvrII CCTAGG 1 cut(s) 842
BalI TGGCCA 1 cut(s) 173
BanII GRGCYC 3 cut(s) 465, 932, 1083
BauI CACGAG 1 cut(s) 55
Bbv12I GWGCWC 3 cut(s) 721, 932, 1083
BbvI GCAGC 5 cut(s) 457, 717, 720, 994, 1083
BccI CCATC 1 cut(s) 913
BceAI ACGGC 2 cut(s) 109, 448
BciT130I CCWGG 3 cut(s) 182, 212, 1051
BclI TGATCA 1 cut(s) 103
BcoDI GTCTC 2 cut(s) 568, 609
BfaI CTAG 2 cut(s) 843, 950
BglII AGATCT 1 cut(s) 37
BisI GCNGC 6 cut(s) 471, 474, 706, 709, 983, 1072
BlnI CCTAGG 1 cut(s) 842
BlsI GCNGC 6 cut(s) 472, 475, 707, 710, 984, 1073
Bme1390I CCNGG 3 cut(s) 182, 212, 1051
Bme18I GGWCC 2 cut(s) 178, 934
BmeT110I CYCGRG 1 cut(s) 1084
BmgT120I GGNCC 5 cut(s) 20, 178, 300, 887, 934
BmiI GGNNCC 2 cut(s) 322, 888
BmrFI CCNGG 3 cut(s) 182, 212, 1051
BmsI GCATC 1 cut(s) 406
BpmI CTGGAG 2 cut(s) 1072, 1176
BsaAI YACGTR 1 cut(s) 133
BsaBI GATNNNNATC 1 cut(s) 219
BsaHI GRCGYC 1 cut(s) 122
BsaJI CCNNGG 3 cut(s) 174, 396, 842
BsaWI WCCGGW 1 cut(s) 323
Bsc4I CCNNNNNNNGG 3 cut(s) 29, 843, 1090
Bse118I RCCGGY 1 cut(s) 889
Bse1I ACTGG 1 cut(s) 302
Bse3DI GCAATG 1 cut(s) 172
Bse8I GATNNNNATC 1 cut(s) 219
BseBI CCWGG 3 cut(s) 182, 212, 1051
BseDI CCNNGG 3 cut(s) 174, 396, 842
BseGI GGATG 1 cut(s) 421
BseJI GATNNNNATC 1 cut(s) 219
BseLI CCNNNNNNNGG 3 cut(s) 29, 843, 1090
BseMI GCAATG 1 cut(s) 172
BseMII CTCAG 1 cut(s) 791
BseNI ACTGG 1 cut(s) 302
BseRI GAGGAG 2 cut(s) 645, 757
BseXI GCAGC 5 cut(s) 457, 717, 720, 994, 1083
BseYI CCCAGC 1 cut(s) 419
BsgI GTGCAG 1 cut(s) 1001
Bsh1236I CGCG 1 cut(s) 84
Bsh1285I CGRYCG 2 cut(s) 30, 82
BshFI GGCC 5 cut(s) 22, 173, 301, 435, 889
BsiEI CGRYCG 2 cut(s) 30, 82
BsiHKAI GWGCWC 3 cut(s) 721, 932, 1083
BsiHKCI CYCGRG 1 cut(s) 1084
BsiSI CCGG 4 cut(s) 318, 324, 612, 890
BslI CCNNNNNNNGG 3 cut(s) 29, 843, 1090
BsmAI GTCTC 2 cut(s) 568, 609
BsnI GGCC 5 cut(s) 22, 173, 301, 435, 889
BsoBI CYCGRG 1 cut(s) 1084
Bsp1286I GDGCHC 4 cut(s) 465, 721, 932, 1083
Bsp1407I TGTACA 1 cut(s) 1185
Bsp143I GATC 9 cut(s) 27, 37, 90, 103, 159, 487, 538, 865, 1062
Bsp19I CCATGG 1 cut(s) 396
BspACI CCGC 7 cut(s) 82, 468, 474, 546, 609, 727, 957
BspANI GGCC 5 cut(s) 22, 173, 301, 435, 889
BspCNI CTCAG 1 cut(s) 790
BspFNI CGCG 1 cut(s) 84
BspLI GGNNCC 2 cut(s) 322, 888
BspPI GGATC 2 cut(s) 873, 1070
BspQI GCTCTTC 1 cut(s) 846
BsrDI GCAATG 1 cut(s) 172
BsrFI RCCGGY 1 cut(s) 889
BsrGI TGTACA 1 cut(s) 1185
BsrI ACTGG 1 cut(s) 302
BssAI RCCGGY 1 cut(s) 889
BssECI CCNNGG 3 cut(s) 174, 396, 842
BssMI GATC 9 cut(s) 27, 37, 90, 103, 159, 487, 538, 865, 1062
BssNI GRCGYC 1 cut(s) 122
BssSI CACGAG 1 cut(s) 55
BssT1I CCWWGG 3 cut(s) 174, 396, 842
Bst2BI CACGAG 1 cut(s) 55
Bst2UI CCWGG 3 cut(s) 182, 212, 1051
Bst4CI ACNGT 1 cut(s) 208
Bst6I CTCTTC 2 cut(s) 357, 846
BstACI GRCGYC 1 cut(s) 122
BstAPI GCANNNNNTGC 1 cut(s) 991
BstAUI TGTACA 1 cut(s) 1185
BstBAI YACGTR 1 cut(s) 133
BstC8I GCNNGC 1 cut(s) 24
BstDEI CTNAG 1 cut(s) 777
BstDSI CCRYGG 1 cut(s) 396
BstEII GGTNACC 1 cut(s) 502
BstF5I GGATG 1 cut(s) 421
BstFNI CGCG 1 cut(s) 84
BstKTI GATC 9 cut(s) 30, 40, 93, 106, 162, 490, 541, 868, 1065
BstMAI GTCTC 2 cut(s) 568, 609
BstMBI GATC 9 cut(s) 27, 37, 90, 103, 159, 487, 538, 865, 1062
BstMCI CGRYCG 2 cut(s) 30, 82
BstMWI GCNNNNNNNGC 2 cut(s) 479, 991
BstNI CCWGG 3 cut(s) 182, 212, 1051
BstPI GGTNACC 1 cut(s) 502
BstSCI CCNGG 3 cut(s) 180, 210, 1049
BstUI CGCG 1 cut(s) 84
BstV1I GCAGC 5 cut(s) 457, 717, 720, 994, 1083
BstX2I RGATCY 2 cut(s) 37, 865
BstYI RGATCY 2 cut(s) 37, 865
BsuRI GGCC 5 cut(s) 22, 173, 301, 435, 889
BtgI CCRYGG 1 cut(s) 396
BtgZI GCGATG 1 cut(s) 79
BtsCI GGATG 1 cut(s) 421
BtsI GCAGTG 3 cut(s) 375, 566, 771
BtsIMutI CAGTG 4 cut(s) 295, 375, 566, 771
Cac8I GCNNGC 1 cut(s) 24
CaiI CAGNNNCTG 1 cut(s) 1050
Cfr10I RCCGGY 1 cut(s) 889
Cfr13I GGNCC 5 cut(s) 20, 178, 300, 887, 934
CseI GACGC 3 cut(s) 49, 73, 130
CsiI ACCWGGT 1 cut(s) 180
Csp6I GTAC 2 cut(s) 805, 1186
CspCI CAANNNNNGTGG 2 cut(s) 325, 360
CviAII CATG 7 cut(s) 107, 190, 289, 397, 862, 938, 1090
CviQI GTAC 2 cut(s) 805, 1186
DdeI CTNAG 1 cut(s) 777
DpnI GATC 9 cut(s) 29, 39, 92, 105, 161, 489, 540, 867, 1064
DpnII GATC 9 cut(s) 27, 37, 90, 103, 159, 487, 538, 865, 1062
DraI TTTAAA 1 cut(s) 1162
DrdI GACNNNNNNGTC 1 cut(s) 500
DseDI GACNNNNNNGTC 1 cut(s) 500
EaeI YGGCCR 1 cut(s) 171
Eam1104I CTCTTC 2 cut(s) 357, 846
EarI CTCTTC 2 cut(s) 357, 846
EciI GGCGGA 1 cut(s) 561
Ecl136II GAGCTC 2 cut(s) 930, 1081
Eco130I CCWWGG 3 cut(s) 174, 396, 842
Eco24I GRGCYC 3 cut(s) 465, 932, 1083
Eco47I GGWCC 2 cut(s) 178, 934
Eco53kI GAGCTC 2 cut(s) 930, 1081
Eco57I CTGAAG 2 cut(s) 402, 995
Eco88I CYCGRG 1 cut(s) 1084
Eco91I GGTNACC 1 cut(s) 502
EcoICRI GAGCTC 2 cut(s) 930, 1081
EcoO65I GGTNACC 1 cut(s) 502
EcoRII CCWGG 3 cut(s) 180, 210, 1049
EcoT14I CCWWGG 3 cut(s) 174, 396, 842
EcoT38I GRGCYC 3 cut(s) 465, 932, 1083
ErhI CCWWGG 3 cut(s) 174, 396, 842
FaeI CATG 7 cut(s) 110, 193, 292, 400, 865, 941, 1093
FalI AAGNNNNNCTT 2 cut(s) 512, 544
FatI CATG 7 cut(s) 106, 189, 288, 396, 861, 937, 1089
FbaI TGATCA 1 cut(s) 103
Fnu4HI GCNGC 6 cut(s) 471, 474, 706, 709, 983, 1072
FokI GGATG 1 cut(s) 428
FriOI GRGCYC 3 cut(s) 465, 932, 1083
Fsp4HI GCNGC 6 cut(s) 471, 474, 706, 709, 983, 1072
FspBI CTAG 2 cut(s) 843, 950
GluI GCNGC 6 cut(s) 471, 474, 706, 709, 983, 1072
GsaI CCCAGC 1 cut(s) 423
GsuI CTGGAG 2 cut(s) 1072, 1176
HaeIII GGCC 5 cut(s) 22, 173, 301, 435, 889
HapII CCGG 4 cut(s) 318, 324, 612, 890
HgaI GACGC 3 cut(s) 49, 73, 130
Hin1I GRCGYC 1 cut(s) 122
Hin1II CATG 7 cut(s) 110, 193, 292, 400, 865, 941, 1093
HincII GTYRAC 2 cut(s) 74, 202
HindII GTYRAC 2 cut(s) 74, 202
HinfI GANTC 7 cut(s) 220, 261, 658, 760, 797, 970, 1054
HpaII CCGG 4 cut(s) 318, 324, 612, 890
HphI GGTGA 2 cut(s) 338, 496
Hpy166II GTNNAC 6 cut(s) 74, 141, 202, 375, 1186, 1188
Hpy188I TCNGA 5 cut(s) 44, 282, 780, 875, 975
Hpy188III TCNNGA 8 cut(s) 88, 224, 491, 529, 671, 679, 1084, 1193
Hpy8I GTNNAC 6 cut(s) 74, 141, 202, 375, 1186, 1188
Hpy99I CGWCG 3 cut(s) 65, 89, 124
HpyAV CCTTC 9 cut(s) 11, 282, 313, 493, 595, 688, 970, 1061, 1121
HpyCH4III ACNGT 1 cut(s) 208
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 6 cut(s) 450, 482, 982, 994, 1023, 1104
HpyF10VI GCNNNNNNNGC 2 cut(s) 479, 991
HpyF3I CTNAG 1 cut(s) 777
HpySE526I ACGT 1 cut(s) 132
Hsp92I GRCGYC 1 cut(s) 122
Hsp92II CATG 7 cut(s) 110, 193, 292, 400, 865, 941, 1093
Ksp22I TGATCA 1 cut(s) 103
Kzo9I GATC 9 cut(s) 27, 37, 90, 103, 159, 487, 538, 865, 1062
LguI GCTCTTC 1 cut(s) 846
LmnI GCTCC 4 cut(s) 326, 1052, 1078, 1086
Lsp1109I GCAGC 5 cut(s) 457, 717, 720, 994, 1083
LweI GCATC 1 cut(s) 406
MabI ACCWGGT 1 cut(s) 180
MaeI CTAG 2 cut(s) 843, 950
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 4 cut(s) 284, 326, 346, 502
MalI GATC 9 cut(s) 29, 39, 92, 105, 161, 489, 540, 867, 1064
MboI GATC 9 cut(s) 27, 37, 90, 103, 159, 487, 538, 865, 1062
MboII GAAGA 8 cut(s) 374, 395, 532, 647, 863, 994, 1121, 1124
MfeI CAATTG 2 cut(s) 576, 786
MflI RGATCY 2 cut(s) 37, 865
MhlI GDGCHC 4 cut(s) 465, 721, 932, 1083
MlsI TGGCCA 1 cut(s) 173
MluCI AATT 5 cut(s) 247, 387, 576, 735, 786
MluNI TGGCCA 1 cut(s) 173
MlyI GAGTC 2 cut(s) 754, 1063
MmeI TCCRAC 1 cut(s) 1013
Mox20I TGGCCA 1 cut(s) 173
MroXI GAANNNNTTC 1 cut(s) 371
MscI TGGCCA 1 cut(s) 173
MseI TTAA 4 cut(s) 438, 858, 1133, 1161
Msp20I TGGCCA 1 cut(s) 173
MspA1I CMGCKG 1 cut(s) 470
MspI CCGG 4 cut(s) 318, 324, 612, 890
MspR9I CCNGG 3 cut(s) 182, 212, 1051
MunI CAATTG 2 cut(s) 576, 786
MvaI CCWGG 3 cut(s) 182, 212, 1051
MvnI CGCG 1 cut(s) 84
MwoI GCNNNNNNNGC 2 cut(s) 479, 991
NcoI CCATGG 1 cut(s) 396
NdeII GATC 9 cut(s) 27, 37, 90, 103, 159, 487, 538, 865, 1062
NlaIII CATG 7 cut(s) 110, 193, 292, 400, 865, 941, 1093
NlaIV GGNNCC 2 cut(s) 322, 888
NmuCI GTSAC 4 cut(s) 284, 326, 346, 502
PciSI GCTCTTC 1 cut(s) 846
PcsI WCGNNNNNNNCGW 1 cut(s) 84
PdmI GAANNNNTTC 1 cut(s) 371
PfeI GAWTC 5 cut(s) 220, 261, 658, 797, 970
PflFI GACNNNGTC 3 cut(s) 83, 124, 182
PfoI TCCNGGA 2 cut(s) 210, 1049
PkrI GCNGC 6 cut(s) 472, 475, 707, 710, 984, 1073
Ple19I CGATCG 1 cut(s) 30
PleI GAGTC 2 cut(s) 754, 1062
PpsI GAGTC 2 cut(s) 754, 1062
Ppu21I YACGTR 1 cut(s) 133
Psp124BI GAGCTC 2 cut(s) 932, 1083
Psp6I CCWGG 3 cut(s) 180, 210, 1049
PspEI GGTNACC 1 cut(s) 502
PspFI CCCAGC 1 cut(s) 419
PspGI CCWGG 3 cut(s) 180, 210, 1049
PspN4I GGNNCC 2 cut(s) 322, 888
PspPI GGNCC 5 cut(s) 20, 178, 300, 887, 934
PstNI CAGNNNCTG 1 cut(s) 1050
PsuI RGATCY 2 cut(s) 37, 865
PsyI GACNNNGTC 3 cut(s) 83, 124, 182
PvuI CGATCG 1 cut(s) 30
RsaI GTAC 2 cut(s) 806, 1187
RsaNI GTAC 2 cut(s) 805, 1186
SacI GAGCTC 2 cut(s) 932, 1083
SapI GCTCTTC 1 cut(s) 846
SaqAI TTAA 4 cut(s) 438, 858, 1133, 1161
SatI GCNGC 6 cut(s) 471, 474, 706, 709, 983, 1072
Sau3AI GATC 9 cut(s) 27, 37, 90, 103, 159, 487, 538, 865, 1062
Sau96I GGNCC 5 cut(s) 20, 178, 300, 887, 934
SchI GAGTC 2 cut(s) 754, 1063
ScrFI CCNGG 3 cut(s) 182, 212, 1051
SduI GDGCHC 4 cut(s) 465, 721, 932, 1083
SexAI ACCWGGT 1 cut(s) 180
SfaNI GCATC 1 cut(s) 406
SinI GGWCC 2 cut(s) 178, 934
Sse9I AATT 5 cut(s) 247, 387, 576, 735, 786
SsiI CCGC 7 cut(s) 82, 468, 474, 546, 609, 727, 957
SspMI CTAG 2 cut(s) 843, 950
SstI GAGCTC 2 cut(s) 932, 1083
StyD4I CCNGG 3 cut(s) 180, 210, 1049
StyI CCWWGG 3 cut(s) 174, 396, 842
TaaI ACNGT 1 cut(s) 208
TaiI ACGT 1 cut(s) 135
TaqI TCGA 5 cut(s) 87, 223, 537, 1057, 1065
TaqII GACCGA 1 cut(s) 922
TasI AATT 5 cut(s) 247, 387, 576, 735, 786
TatI WGTACW 1 cut(s) 1185
TauI GCSGC 1 cut(s) 476
TfiI GAWTC 5 cut(s) 220, 261, 658, 797, 970
Tru1I TTAA 4 cut(s) 438, 858, 1133, 1161
Tru9I TTAA 4 cut(s) 438, 858, 1133, 1161
TscAI CASTG 4 cut(s) 302, 382, 573, 778
TseFI GTSAC 4 cut(s) 284, 326, 346, 502
TseI GCWGC 5 cut(s) 470, 705, 708, 982, 1071
Tsp45I GTSAC 4 cut(s) 284, 326, 346, 502
TspDTI ATGAA 5 cut(s) 206, 305, 470, 671, 1140
TspGWI ACGGA 2 cut(s) 164, 435
TspRI CASTG 4 cut(s) 302, 382, 573, 778
Tth111I GACNNNGTC 3 cut(s) 83, 124, 182
VpaK11BI GGWCC 2 cut(s) 178, 934
XapI RAATTY 2 cut(s) 247, 387
XmaJI CCTAGG 1 cut(s) 842
XmnI GAANNNNTTC 1 cut(s) 371
XspI CTAG 2 cut(s) 843, 950
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.