MD08G1166300.v1.1

prolyl-tRNA synthetase associated domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
N/A
Physical Location & Seq
Forward (+)
19729013 .. 19730436
1424 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1166300.v1.1.491

Sequence Viewer

Length: 354 bp
ATGATTTGTTTTCATCGGGCCAAATATGTTGGAAATCTGGGAGGTGGACTGAGTGAAAATTTATTCTTGACGGACAAGAAAAGAAGGTTTTATAATTTTTCCGCTTCGGCAGATGCTAAATTAGATTTGAAAGCGGTTCTATCTGTGGGGCTTGGTTTGGGAAGAGGGGGCTTGAGAATGGCTCCTGAAGAAGCACTTGGAGAAATACTTCAGGTCCCTCTGGGTTGCGTTATGCCTTTTTCCCTGGTGAATGAATCTGGACGCCATGTCTCTCTGTTGTTGGATCACAAATTTAAAAGTCAGGAGTGGTGCTTCTTCCACCCTTTATCGAATGACATGTCAATCTGTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2 tRNA_edit

Protein Analysis

118

Amino Acids

12.95

Weight (kDa)

8.76

Isoelectric Point (pI)

47.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_edit PF04073 9 - 114 4.8e-14 Aminoacyl-tRNA editing domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 93
AciI CCGC 2 cut(s) 102, 134
AclWI GGATC 1 cut(s) 291
AcsI RAATTY 2 cut(s) 58, 290
AcuI CTGAAG 2 cut(s) 194, 207
AcyI GRCGYC 1 cut(s) 262
AflIII ACRYGT 1 cut(s) 336
AgsI TTSAA 1 cut(s) 130
AhdI GACNNNNNGTC 1 cut(s) 266
AjnI CCWGG 1 cut(s) 243
AjuI GAANNNNNNNTTGG 2 cut(s) 180, 212
Alw26I GTCTC 1 cut(s) 274
AlwI GGATC 1 cut(s) 291
AoxI GGCC 1 cut(s) 18
ApoI RAATTY 2 cut(s) 58, 290
ArsI GACNNNNNNTTYG 1 cut(s) 323
Asp700I GAANNNNTTC 1 cut(s) 207
AspS9I GGNCC 2 cut(s) 18, 214
AsuHPI GGTGA 1 cut(s) 259
AvaII GGWCC 1 cut(s) 214
BciT130I CCWGG 1 cut(s) 245
BcoDI GTCTC 1 cut(s) 274
Bme1390I CCNGG 1 cut(s) 245
Bme18I GGWCC 1 cut(s) 214
BmeRI GACNNNNNGTC 1 cut(s) 266
BmgT120I GGNCC 2 cut(s) 18, 214
BmiI GGNNCC 2 cut(s) 183, 216
BmrFI CCNGG 1 cut(s) 245
BmsI GCATC 1 cut(s) 103
BplI GAGNNNNNCTC 2 cut(s) 166, 198
BpuEI CTTGAG 1 cut(s) 193
BsaHI GRCGYC 1 cut(s) 262
BsaJI CCNNGG 1 cut(s) 243
BseBI CCWGG 1 cut(s) 245
BseDI CCNNGG 1 cut(s) 243
BseMII CTCAG 1 cut(s) 41
BshFI GGCC 1 cut(s) 20
BslFI GGGAC 1 cut(s) 200
BsmAI GTCTC 1 cut(s) 274
BsmFI GGGAC 1 cut(s) 200
BsnI GGCC 1 cut(s) 20
Bsp143I GATC 1 cut(s) 283
BspACI CCGC 2 cut(s) 102, 134
BspANI GGCC 1 cut(s) 20
BspCNI CTCAG 1 cut(s) 42
BspLI GGNNCC 2 cut(s) 183, 216
BspPI GGATC 1 cut(s) 291
BssECI CCNNGG 1 cut(s) 243
BssMI GATC 1 cut(s) 283
BssNI GRCGYC 1 cut(s) 262
Bst2UI CCWGG 1 cut(s) 245
Bst6I CTCTTC 1 cut(s) 157
BstACI GRCGYC 1 cut(s) 262
BstDEI CTNAG 1 cut(s) 50
BstKTI GATC 1 cut(s) 286
BstMAI GTCTC 1 cut(s) 274
BstMBI GATC 1 cut(s) 283
BstNI CCWGG 1 cut(s) 245
BstNSI RCATGY 1 cut(s) 340
BstSCI CCNGG 1 cut(s) 243
BsuRI GGCC 1 cut(s) 20
Cfr13I GGNCC 2 cut(s) 18, 214
CseI GACGC 1 cut(s) 270
CviAII CATG 2 cut(s) 266, 337
CviJI RGCY 4 cut(s) 20, 151, 171, 182
CviKI_1 RGCY 4 cut(s) 20, 151, 171, 182
DdeI CTNAG 1 cut(s) 50
DpnI GATC 1 cut(s) 285
DpnII GATC 1 cut(s) 283
DraI TTTAAA 1 cut(s) 295
DriI GACNNNNNGTC 1 cut(s) 266
Eam1104I CTCTTC 1 cut(s) 157
Eam1105I GACNNNNNGTC 1 cut(s) 266
EarI CTCTTC 1 cut(s) 157
Eco47I GGWCC 1 cut(s) 214
Eco57I CTGAAG 2 cut(s) 194, 207
EcoO109I RGGNCCY 1 cut(s) 214
EcoRII CCWGG 1 cut(s) 243
FaeI CATG 2 cut(s) 269, 340
FaiI YATR 5 cut(s) 27, 93, 233, 267, 338
FalI AAGNNNNNCTT 2 cut(s) 180, 212
FaqI GGGAC 1 cut(s) 200
FatI CATG 2 cut(s) 265, 336
HaeIII GGCC 1 cut(s) 20
HgaI GACGC 1 cut(s) 270
Hin1I GRCGYC 1 cut(s) 262
Hin1II CATG 2 cut(s) 269, 340
HinfI GANTC 1 cut(s) 254
HphI GGTGA 1 cut(s) 259
Hpy166II GTNNAC 1 cut(s) 47
Hpy188III TCNNGA 4 cut(s) 67, 185, 258, 302
Hpy8I GTNNAC 1 cut(s) 47
HpyAV CCTTC 1 cut(s) 78
HpyF3I CTNAG 1 cut(s) 50
Hsp92I GRCGYC 1 cut(s) 262
Hsp92II CATG 2 cut(s) 269, 340
Kzo9I GATC 1 cut(s) 283
LmnI GCTCC 1 cut(s) 187
LpnPI CCDG 8 cut(s) 23, 197, 198, 206, 230, 243, 257, 287
LweI GCATC 1 cut(s) 103
MalI GATC 1 cut(s) 285
MboI GATC 1 cut(s) 283
MboII GAAGA 3 cut(s) 174, 200, 307
MluCI AATT 4 cut(s) 58, 94, 119, 290
MmeI TCCRAC 2 cut(s) 10, 261
MnlI CCTC 3 cut(s) 35, 158, 228
MroXI GAANNNNTTC 1 cut(s) 207
MseI TTAA 1 cut(s) 294
MspR9I CCNGG 1 cut(s) 245
MvaI CCWGG 1 cut(s) 245
NdeII GATC 1 cut(s) 283
NlaIII CATG 2 cut(s) 269, 340
NlaIV GGNNCC 2 cut(s) 183, 216
NspI RCATGY 1 cut(s) 340
PciI ACATGT 1 cut(s) 336
PdmI GAANNNNTTC 1 cut(s) 207
PfeI GAWTC 1 cut(s) 254
PpuMI RGGWCCY 1 cut(s) 214
PscI ACATGT 1 cut(s) 336
PsiI TTATAA 1 cut(s) 93
Psp5II RGGWCCY 1 cut(s) 214
Psp6I CCWGG 1 cut(s) 243
PspGI CCWGG 1 cut(s) 243
PspN4I GGNNCC 2 cut(s) 183, 216
PspPI GGNCC 2 cut(s) 18, 214
PspPPI RGGWCCY 1 cut(s) 214
SaqAI TTAA 1 cut(s) 294
Sau3AI GATC 1 cut(s) 283
Sau96I GGNCC 2 cut(s) 18, 214
ScrFI CCNGG 1 cut(s) 245
SetI ASST 3 cut(s) 46, 89, 216
SfaNI GCATC 1 cut(s) 103
SinI GGWCC 1 cut(s) 214
SmlI CTYRAG 1 cut(s) 172
SmoI CTYRAG 1 cut(s) 172
Sse9I AATT 4 cut(s) 58, 94, 119, 290
SsiI CCGC 2 cut(s) 102, 134
StyD4I CCNGG 1 cut(s) 243
TaqI TCGA 1 cut(s) 329
TasI AATT 4 cut(s) 58, 94, 119, 290
TfiI GAWTC 1 cut(s) 254
Tru1I TTAA 1 cut(s) 294
Tru9I TTAA 1 cut(s) 294
TspDTI ATGAA 1 cut(s) 267
TspGWI ACGGA 1 cut(s) 86
VpaK11BI GGWCC 1 cut(s) 214
XapI RAATTY 2 cut(s) 58, 290
XceI RCATGY 1 cut(s) 340
XmnI GAANNNNTTC 1 cut(s) 207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.