MD09G1218200.v1.1
HSP70 Family

Belongs to the heat shock protein 70 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
N/A
Physical Location & Seq
Reverse (-)
21965878 .. 21972737
6860 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1218200.v1.1.491

Sequence Viewer

Length: 1128 bp
ATGGCCGCCGCTGCTATGATCCGTAACTTCCGGCGCCGCGACGTCGCCTCGGCCTCCCTTTCCGCCTACTGTTCCTTACTTCTAGACCTGCTGGGAATGATTGGGATTGACTTGGGCACAACAAACTCATGTGTTGTTGTGATGGAGGGAAACGTAAACCCCTCGGACCACACCATCAGTAGTTACTATGAACCAGAAAGGAGAACTACTTGTTGGTGCTCCAACAAAACGTCAAGCTGTGACGAACCCAGCAAACGTCAAGCTGTGACGAACCCAACAAACACAGTTTCTGGAACCAAGCGTCTGATTGGTATACACTATGACGATGCCCAATCACAAAAGGAATTGAAGAAGGTTCCATACAAGATTGTTAAAGCTCCAAATGGAGATGCATGGGTTGAAGTCAATCGACAGCAGTATGCTCCAGGCCAGATTGGAGCTTTTGTTCTGACAAAGATGAAAGAAACTGCTGAGGCTTACCTTGGAAAGTCTGTTTCGAGAGCTGTGATCATGGTGAAAGCAACAAATGGTGACACATTCTCGGGAGGAGAGGATTTTGACAATGCTTTGCTGGACTTCTTAGTGACCGATGCTTCGGGTGCCAAGCATCTGCACATCACGCTGACCCGATCAAAGTTTGAAAGCCTGGTTAATCACCTGATTGAAAGGACAAAGGTGCCCTGCAAGAATTGTTTGAAGGATGCCAACATGTCCATTAAGGATGTGGATGAGGTCCTTCTCGTCGGAGGGATGACTCGCGTTCCTAAAGTGCAAGAGGTGGTTGCAGAAATATTTGGAAAGAGCCCAAGCAAAGGAGTCAATCCCGATGAGGCAGTTGCTTTGGGAGCTGCTATCCAAGGTGGTATTCTTTACGGTGATGTTATGGAGTTGCTTCTTCTCGATGTTAATCCTCTCTCACTCGGTATTTCGACAGCAGCTGACAACCAAACCCAGGTCGGTATCAAGGTGCTACAAGGAGAGCGCAAAATGGCTAACAAGCTTCTTGGAGAGTTTGCCCTTCAAGGCATCCCTCCAGCACAGAGGGGCTTGCCTCAGATTGAAGTTACATTTGACATTGACGCCAAAGGTATTGTAACTGTTTTCTGCAAGGGACAAGGCAACTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03018 ko04212 ko05152 map03018 map04212 map05152
Pfam Domains
Protein Families
HSP70

Protein Analysis

376

Amino Acids

40.52

Weight (kDa)

6.95

Isoelectric Point (pI)

35.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 33 - 172 1e-35 Hsp70 protein
HSP70 PF00012 197 - 312 4.4e-49 Hsp70 protein
FGGY_C PF02782 226 - 289 7.5e-06 FGGY family of carbohydrate kinases, C-terminal domain
HSP70 PF00012 310 - 370 1.1e-19 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 45
Acc36I ACCTGC 1 cut(s) 96
AccB1I GGYRCC 3 cut(s) 33, 599, 676
AccI GTMKAC 1 cut(s) 313
AccII CGCG 2 cut(s) 39, 759
AciI CCGC 4 cut(s) 6, 9, 37, 63
AclWI GGATC 1 cut(s) 13
AcoI YGGCCR 1 cut(s) 3
AcyI GRCGYC 3 cut(s) 34, 42, 1080
AfiI CCNNNNNNNGG 2 cut(s) 812, 952
AflIII ACRYGT 1 cut(s) 708
AgsI TTSAA 7 cut(s) 349, 401, 641, 665, 697, 1022, 1061
AjnI CCWGG 3 cut(s) 424, 645, 951
AjuI GAANNNNNNNTTGG 4 cut(s) 196, 228, 849, 881
AloI GAACNNNNNNTCC 2 cut(s) 429, 461
AluBI AGCT 8 cut(s) 237, 263, 377, 440, 503, 848, 938, 1000
AluI AGCT 8 cut(s) 237, 263, 377, 440, 503, 848, 938, 1000
Alw21I GWGCWC 1 cut(s) 221
AlwI GGATC 1 cut(s) 13
AlwNI CAGNNNCTG 2 cut(s) 290, 938
Ama87I CYCGRG 1 cut(s) 541
AoxI GGCC 3 cut(s) 3, 51, 427
ApeKI GCWGC 3 cut(s) 11, 848, 935
ArsI GACNNNNNNTTYG 2 cut(s) 577, 609
AspLEI GCGC 2 cut(s) 36, 984
AspS9I GGNCC 2 cut(s) 166, 733
AsuHPI GGTGA 4 cut(s) 526, 542, 647, 887
AvaI CYCGRG 1 cut(s) 541
AvaII GGWCC 2 cut(s) 166, 733
BaeGI GKGCMC 2 cut(s) 119, 681
BanI GGYRCC 3 cut(s) 33, 599, 676
BanII GRGCYC 1 cut(s) 806
BarI GAAGNNNNNNTAC 2 cut(s) 344, 376
Bbv12I GWGCWC 1 cut(s) 221
BbvCI CCTCAGC 1 cut(s) 471
BbvI GCAGC 2 cut(s) 835, 947
BccI CCATC 2 cut(s) 136, 182
BciT130I CCWGG 3 cut(s) 426, 647, 953
BclI TGATCA 1 cut(s) 507
BfaI CTAG 1 cut(s) 83
BfoI RGCGCY 1 cut(s) 37
BfuAI ACCTGC 1 cut(s) 96
BisI GCNGC 6 cut(s) 6, 9, 12, 37, 849, 936
BlsI GCNGC 6 cut(s) 7, 10, 13, 38, 850, 937
Bme1390I CCNGG 3 cut(s) 426, 647, 953
Bme18I GGWCC 2 cut(s) 166, 733
BmeT110I CYCGRG 1 cut(s) 541
BmgT120I GGNCC 2 cut(s) 166, 733
BmiI GGNNCC 5 cut(s) 35, 295, 357, 601, 678
BmrFI CCNGG 3 cut(s) 426, 647, 953
BmsI GCATC 6 cut(s) 316, 379, 580, 616, 691, 1035
BpmI CTGGAG 2 cut(s) 408, 1017
Bpu10I CCTNAGC 1 cut(s) 471
BsaBI GATNNNNATC 1 cut(s) 906
BsaHI GRCGYC 3 cut(s) 34, 42, 1080
BsaJI CCNNGG 5 cut(s) 48, 162, 481, 856, 951
BsaXI ACNNNNNCTCC 2 cut(s) 429, 459
Bsc4I CCNNNNNNNGG 2 cut(s) 812, 952
Bse1I ACTGG 1 cut(s) 1127
Bse8I GATNNNNATC 1 cut(s) 906
BseBI CCWGG 3 cut(s) 426, 647, 953
BseDI CCNNGG 5 cut(s) 48, 162, 481, 856, 951
BseGI GGATG 5 cut(s) 706, 727, 733, 756, 1026
BseJI GATNNNNATC 1 cut(s) 906
BseLI CCNNNNNNNGG 2 cut(s) 812, 952
BseMII CTCAG 2 cut(s) 462, 1067
BseNI ACTGG 1 cut(s) 1127
BseRI GAGGAG 1 cut(s) 561
BseSI GKGCMC 2 cut(s) 119, 681
BseXI GCAGC 2 cut(s) 835, 947
BseYI CCCAGC 2 cut(s) 91, 248
BsgI GTGCAG 1 cut(s) 596
Bsh1236I CGCG 2 cut(s) 39, 759
BshFI GGCC 3 cut(s) 5, 53, 429
BshNI GGYRCC 3 cut(s) 33, 599, 676
BsiHKAI GWGCWC 1 cut(s) 221
BsiHKCI CYCGRG 1 cut(s) 541
BsiSI CCGG 1 cut(s) 31
BslI CCNNNNNNNGG 2 cut(s) 812, 952
BsnI GGCC 3 cut(s) 5, 53, 429
BsoBI CYCGRG 1 cut(s) 541
Bsp1286I GDGCHC 4 cut(s) 119, 221, 681, 806
Bsp143I GATC 3 cut(s) 18, 507, 629
BspACI CCGC 4 cut(s) 6, 9, 37, 63
BspANI GGCC 3 cut(s) 5, 53, 429
BspCNI CTCAG 2 cut(s) 463, 1066
BspFNI CGCG 2 cut(s) 39, 759
BspLI GGNNCC 5 cut(s) 35, 295, 357, 601, 678
BspMI ACCTGC 1 cut(s) 96
BspPI GGATC 1 cut(s) 13
BspT107I GGYRCC 3 cut(s) 33, 599, 676
BsrI ACTGG 1 cut(s) 1127
BssECI CCNNGG 5 cut(s) 48, 162, 481, 856, 951
BssMI GATC 3 cut(s) 18, 507, 629
BssNAI GTATAC 1 cut(s) 314
BssNI GRCGYC 3 cut(s) 34, 42, 1080
BssT1I CCWWGG 2 cut(s) 481, 856
Bst1107I GTATAC 1 cut(s) 314
Bst2UI CCWGG 3 cut(s) 426, 647, 953
Bst4CI ACNGT 4 cut(s) 71, 286, 875, 1099
BstACI GRCGYC 3 cut(s) 34, 42, 1080
BstC8I GCNNGC 1 cut(s) 1049
BstDEI CTNAG 3 cut(s) 471, 580, 1053
BstF5I GGATG 5 cut(s) 706, 727, 733, 756, 1026
BstFNI CGCG 2 cut(s) 39, 759
BstH2I RGCGCY 1 cut(s) 37
BstHHI GCGC 2 cut(s) 36, 984
BstKTI GATC 3 cut(s) 21, 510, 632
BstMBI GATC 3 cut(s) 18, 507, 629
BstMWI GCNNNNNNNGC 4 cut(s) 11, 599, 619, 845
BstNI CCWGG 3 cut(s) 426, 647, 953
BstNSI RCATGY 1 cut(s) 712
BstSCI CCNGG 3 cut(s) 424, 645, 951
BstSLI GKGCMC 2 cut(s) 119, 681
BstUI CGCG 2 cut(s) 39, 759
BstV1I GCAGC 2 cut(s) 835, 947
BstZ17I GTATAC 1 cut(s) 314
BsuRI GGCC 3 cut(s) 5, 53, 429
BtsCI GGATG 5 cut(s) 706, 727, 733, 756, 1026
BveI ACCTGC 1 cut(s) 96
Cac8I GCNNGC 1 cut(s) 1049
CaiI CAGNNNCTG 2 cut(s) 290, 938
CfoI GCGC 2 cut(s) 36, 984
Cfr13I GGNCC 2 cut(s) 166, 733
CseI GACGC 2 cut(s) 290, 1088
CviAII CATG 4 cut(s) 129, 393, 511, 709
DdeI CTNAG 3 cut(s) 471, 580, 1053
DinI GGCGCC 1 cut(s) 35
DpnI GATC 3 cut(s) 20, 509, 631
DpnII GATC 3 cut(s) 18, 507, 629
EaeI YGGCCR 1 cut(s) 3
EciI GGCGGA 1 cut(s) 52
Eco130I CCWWGG 2 cut(s) 481, 856
Eco24I GRGCYC 1 cut(s) 806
Eco47I GGWCC 2 cut(s) 166, 733
Eco88I CYCGRG 1 cut(s) 541
EcoO109I RGGNCCY 1 cut(s) 733
EcoRII CCWGG 3 cut(s) 424, 645, 951
EcoT14I CCWWGG 2 cut(s) 481, 856
EcoT22I ATGCAT 1 cut(s) 394
EcoT38I GRGCYC 1 cut(s) 806
EgeI GGCGCC 1 cut(s) 35
EheI GGCGCC 1 cut(s) 35
ErhI CCWWGG 2 cut(s) 481, 856
FaeI CATG 4 cut(s) 132, 396, 514, 712
FatI CATG 4 cut(s) 128, 392, 510, 708
FbaI TGATCA 1 cut(s) 507
FblI GTMKAC 1 cut(s) 313
Fnu4HI GCNGC 6 cut(s) 6, 9, 12, 37, 849, 936
FokI GGATG 5 cut(s) 713, 734, 740, 763, 1013
FriOI GRGCYC 1 cut(s) 806
Fsp4HI GCNGC 6 cut(s) 6, 9, 12, 37, 849, 936
FspBI CTAG 1 cut(s) 83
GlaI GCGC 2 cut(s) 35, 983
GluI GCNGC 6 cut(s) 6, 9, 12, 37, 849, 936
GsaI CCCAGC 2 cut(s) 95, 252
GsuI CTGGAG 2 cut(s) 408, 1017
HaeII RGCGCY 1 cut(s) 37
HaeIII GGCC 3 cut(s) 5, 53, 429
HapII CCGG 1 cut(s) 31
HgaI GACGC 2 cut(s) 290, 1088
HhaI GCGC 2 cut(s) 36, 984
Hin1I GRCGYC 3 cut(s) 34, 42, 1080
Hin1II CATG 4 cut(s) 132, 396, 514, 712
Hin6I GCGC 2 cut(s) 34, 982
HinP1I GCGC 2 cut(s) 34, 982
HindIII AAGCTT 1 cut(s) 998
HinfI GANTC 2 cut(s) 754, 816
HpaII CCGG 1 cut(s) 31
HphI GGTGA 4 cut(s) 526, 542, 647, 887
Hpy166II GTNNAC 2 cut(s) 157, 314
Hpy188I TCNGA 5 cut(s) 166, 306, 450, 746, 1056
Hpy188III TCNNGA 6 cut(s) 83, 291, 498, 543, 824, 899
Hpy8I GTNNAC 2 cut(s) 157, 314
Hpy99I CGWCG 3 cut(s) 44, 47, 746
HpyAV CCTTC 4 cut(s) 346, 691, 746, 1028
HpyCH4III ACNGT 4 cut(s) 71, 286, 875, 1099
HpyCH4IV ACGT 4 cut(s) 42, 153, 230, 256
HpyCH4V TGCA 6 cut(s) 392, 613, 684, 772, 785, 1107
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 599, 619, 845
HpyF3I CTNAG 3 cut(s) 471, 580, 1053
HpySE526I ACGT 4 cut(s) 42, 153, 230, 256
Hsp92I GRCGYC 3 cut(s) 34, 42, 1080
Hsp92II CATG 4 cut(s) 132, 396, 514, 712
HspAI GCGC 2 cut(s) 34, 982
KasI GGCGCC 1 cut(s) 33
Ksp22I TGATCA 1 cut(s) 507
Kzo9I GATC 3 cut(s) 18, 507, 629
LmnI GCTCC 5 cut(s) 224, 382, 427, 437, 845
Lsp1109I GCAGC 2 cut(s) 835, 947
LweI GCATC 6 cut(s) 316, 379, 580, 616, 691, 1035
MaeI CTAG 1 cut(s) 83
MaeII ACGT 4 cut(s) 42, 153, 230, 256
MaeIII GTNAC 8 cut(s) 23, 182, 239, 265, 530, 583, 1063, 1093
MalI GATC 3 cut(s) 20, 509, 631
MboI GATC 3 cut(s) 18, 507, 629
MboII GAAGA 2 cut(s) 361, 887
MhlI GDGCHC 4 cut(s) 119, 221, 681, 806
MluCI AATT 2 cut(s) 344, 688
Mly113I GGCGCC 1 cut(s) 34
MlyI GAGTC 2 cut(s) 748, 825
MmeI TCCRAC 2 cut(s) 246, 724
Mph1103I ATGCAT 1 cut(s) 394
MseI TTAA 4 cut(s) 372, 651, 717, 906
MspA1I CMGCKG 2 cut(s) 11, 938
MspI CCGG 1 cut(s) 31
MspR9I CCNGG 3 cut(s) 426, 647, 953
MvaI CCWGG 3 cut(s) 426, 647, 953
MvnI CGCG 2 cut(s) 39, 759
MwoI GCNNNNNNNGC 4 cut(s) 11, 599, 619, 845
NarI GGCGCC 1 cut(s) 34
NdeII GATC 3 cut(s) 18, 507, 629
NlaIII CATG 4 cut(s) 132, 396, 514, 712
NlaIV GGNNCC 5 cut(s) 35, 295, 357, 601, 678
NmeAIII GCCGAG 1 cut(s) 29
NmuCI GTSAC 4 cut(s) 239, 265, 530, 583
NsiI ATGCAT 1 cut(s) 394
NspI RCATGY 1 cut(s) 712
PciI ACATGT 1 cut(s) 708
PkrI GCNGC 6 cut(s) 7, 10, 13, 38, 850, 937
PleI GAGTC 2 cut(s) 748, 824
PluTI GGCGCC 1 cut(s) 37
PpsI GAGTC 2 cut(s) 748, 824
PpuMI RGGWCCY 1 cut(s) 733
PscI ACATGT 1 cut(s) 708
Psp5II RGGWCCY 1 cut(s) 733
Psp6I CCWGG 3 cut(s) 424, 645, 951
PspFI CCCAGC 2 cut(s) 91, 248
PspGI CCWGG 3 cut(s) 424, 645, 951
PspN4I GGNNCC 5 cut(s) 35, 295, 357, 601, 678
PspPI GGNCC 2 cut(s) 166, 733
PspPPI RGGWCCY 1 cut(s) 733
PstNI CAGNNNCTG 2 cut(s) 290, 938
PvuII CAGCTG 1 cut(s) 938
SaqAI TTAA 4 cut(s) 372, 651, 717, 906
SatI GCNGC 6 cut(s) 6, 9, 12, 37, 849, 936
Sau3AI GATC 3 cut(s) 18, 507, 629
Sau96I GGNCC 2 cut(s) 166, 733
SchI GAGTC 2 cut(s) 748, 825
ScrFI CCNGG 3 cut(s) 426, 647, 953
SduI GDGCHC 4 cut(s) 119, 221, 681, 806
SfaNI GCATC 6 cut(s) 316, 379, 580, 616, 691, 1035
SfoI GGCGCC 1 cut(s) 35
SinI GGWCC 2 cut(s) 166, 733
Sse9I AATT 2 cut(s) 344, 688
SsiI CCGC 4 cut(s) 6, 9, 37, 63
SspDI GGCGCC 1 cut(s) 33
SspI AATATT 1 cut(s) 792
SspMI CTAG 1 cut(s) 83
StyD4I CCNGG 3 cut(s) 424, 645, 951
StyI CCWWGG 2 cut(s) 481, 856
TaaI ACNGT 4 cut(s) 71, 286, 875, 1099
TaiI ACGT 4 cut(s) 45, 156, 233, 259
TaqI TCGA 4 cut(s) 409, 497, 900, 929
TaqII GACCGA 1 cut(s) 602
TasI AATT 2 cut(s) 344, 688
TauI GCSGC 3 cut(s) 8, 11, 39
Tru1I TTAA 4 cut(s) 372, 651, 717, 906
Tru9I TTAA 4 cut(s) 372, 651, 717, 906
TseFI GTSAC 4 cut(s) 239, 265, 530, 583
TseI GCWGC 3 cut(s) 11, 848, 935
Tsp45I GTSAC 4 cut(s) 239, 265, 530, 583
TspDTI ATGAA 2 cut(s) 204, 473
TspGWI ACGGA 1 cut(s) 11
VpaK11BI GGWCC 2 cut(s) 166, 733
XbaI TCTAGA 1 cut(s) 82
XceI RCATGY 1 cut(s) 712
XcmI CCANNNNNNNNNTGG 1 cut(s) 721
XmiI GTMKAC 1 cut(s) 313
XspI CTAG 1 cut(s) 83
ZraI GACGTC 1 cut(s) 43
Zsp2I ATGCAT 1 cut(s) 394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.