Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

N/A

Physical Location & Seq

Genomic Coordinates Forward (+)

5870055 .. 5876852

Gene Structure 6798 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1043500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2268 bp

ATGAAAGGGGAAAAACCGAGTCCTTTGGGTCATTCTAGGGACAAAGGAAGTGGAAGTTACAGGTTTAAAGAGGAAAAACAAAGATCAATGGAAGCACTGGTTAATGGAAAGTTTAAGGCTCTGATCAGTCAGCTACTTAGATCCGCGGGTGTTGCATCTTATGAGGAGGGCGGTGAGAGTTGGGTGGAAGTTATTGCTTCTTTGTCATGGGAAGCTGCTTCACTTTTGAAACCTGATGCTATTGTTGCCAAAGCAAGGGATCTAGATGGCTACGTGAAAGTAAAATGCATTGCAACTGGTGTTCGCAGCCAAAGTCAACTAGTAAAAGGTTTGGTCTTCAAAAAGCACGCTGCACACAAGCTTATGCCGACCAAGTACGAGAATCCAAGGTTGTTGCTAATCCAGGGAGTACTTGGTCAGTCTTCAGGCGGATTGTCATCCTTTGATTCAATGGAGCTGAGTAAAACGTTAATGTTTATTGAGGACTCTTGTACATGCCTGGGCTGTACGGCTTTGCTGAAAGGAGCACAGAGTGATGCATTGAAGATCAAGTCTGTAGTGCAGTGTGCAGTTTTTCTGGCATATCATTTAATTCTCGAGACTGCTTTCTTTGTTGACCAGAGAGCTACACTTTCATATGCTGATCTAGCAACTGATTTGCCCACTGATACGGAAGCCCATACTTTAGGATCTATTAACTCAGGTGTTCCTCAGAATACGGACACTAGTGCTGAAAATGAATCTGATGCGGTCGATATTCTCATTTCCAATGAATCCCATGAAGGATGTTCTGGCAGTTCTACCTTGGAATTCAAAGGCAACTCCACATTCGACGTACCACGCAATCTTGCCCTAATTTCTGGGTTCTCGTCCATTTTGGTGGCACTAAGGAAGGTTATGGGGGAAGGTTACAGGACTCTCTGGTTTCAGAATGTGGATAGGCTAAGCGAGCTGCCTTTCGACATCGTACGCCATATTCTCTCCCGACTCGCACCTAGGGACATAGCATGCGCCTGCACTCTTTCGAAGACATGGCATGGGTGGTTGACTACCCTTTGCCATGATATTGGCTCTTTAAGGGAGAGTGAATTTCGAAGTTTGGCTGAAGCAAAACTCAGTCTCAGCTTGGAAGGCCCTCTTGTCAAGTGCACTGTAGATTGTCACAAGCATACCGAGCCAAACAGATTGAACGATCTCGTCGATCGCATTTTGGAAGGAGGTGTGCGGATCTTGGGGATTTCCATAGCCCCCTACCTCGGGGACTTTTATGAGCTTCCAGCCCGAGTTGGTACAAGTTCATCTGTACAAGTTATGGAGCTAGCTAGGATGAGGGTTGATCTTCCAGCAGGTTGTTTGAAAAATCTGTCAGTACTACGTATTGATCTGAGTTATGCTTCGGACCCTTCGATGCGAAGCTTCTTCCACAATTGTCCTCGTTTGAAAGTCCTTCACATTGAAGGACAGATATGCGAAGTGCCGAAAGAGAAACAAATGATCAACATTAATTCCATAAGTTTGATGGAATTACGGATCCGCTTGCACATTCAGTTGGACAATCGCGATGCCATTGGTATGCGGACCTCATATGCATTCAAGGTAGCTGCTCCAAATTTGGGTCTCTTGGATTTGGATGACACCACTTGTGCAGCCTTCGGCTTTCAAGGCTCCGTAATGCGTGAGGCTAGAGTTCAGGTCGGCTTATTCCTCGAGGGGCTGTCTCGTCCATTGGAAAGCGACAAGGACGTGTTAGAGCAGATATGTGAAGAGGAAGTAGAAGAAGTTGGGAGCACTGTGTTTGGTTTATCCGAGAGCCCATGTCTTAACTCACTCATCCTGGGCGATAGAACGATGGGGGCGTTAGGACATATTTTTGTCTGGGACGTTCTACGTTATGAGCCCCTTTGGACGGACTCTGATGTGGACATTGCTCGGAGGTACAAAAGGAGATTTCCTGTTTTTCCGGCTGTGAAGAGCCTCACAGTGCAGATCTCACATGGGTATGGATGGCTGTTGTTGCCATTCCTGGTGACGAGCTTTACAATGCTGGAAGTACTGTCTATAGAAAAGGTGGTGAGGGAGCATGTGAGCATGAGTTTTGACTGGAGACCACCGGAAGAACCAACGCCAGCATGCTTGTTGACAAGTGTCCGGGAGATCAACCTCATTGGATTTCAGGAATCGAAGGATGAGGCTGATAGGTTGATGGCGTATTTCAAGAGTATTCTGAAGTTTAGAAGAGACCAGGGATTCGAAATCAAGCTTCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000285 GO:0000322 GO:0000323 GO:0000324 GO:0000329 GO:0003674 GO:0003824 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005942 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006810 GO:0008150 GO:0008152 GO:0008289 GO:0009987 GO:0010008 GO:0012505 GO:0016020 GO:0016192 GO:0016197 GO:0016301 GO:0016307 GO:0016310 GO:0016482 GO:0016740 GO:0016772 GO:0016773 GO:0019637 GO:0019898 GO:0030258 GO:0031090 GO:0031410 GO:0031982 GO:0032266 GO:0032991 GO:0035032 GO:0035091 GO:0042147 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044255 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0046486 GO:0046488 GO:0046834 GO:0046854 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061695 GO:0070772 GO:0071704 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0098852 GO:1901981 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00562 ko04070 ko04145 ko04810 map00562 map04070 map04145 map04810

Pfam Domains

Protein Families

Cpn60_TCP1 PIP5K

Protein Analysis

756

Amino Acids

83.81

Weight (kDa)

5.94

Isoelectric Point (pI)

45.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cpn60_TCP1	PF00118	58 - 139	3.8e-06	TCP-1/cpn60 chaperonin family
F-box	PF00646	315 - 349	3.9e-06	F-box domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AbsI	CCTCGAGG	1 cut(s)	1706
Acc36I	ACCTGC	1 cut(s)	1337
AccII	CGCG	2 cut(s)	146, 1560
AciI	CCGC	8 cut(s)	144, 146, 171, 429, 749, 1225, 1534, 1576
AclI	AACGTT	1 cut(s)	467
AclWI	GGATC	6 cut(s)	135, 267, 697, 1235, 1525, 1538
AcsI	RAATTY	3 cut(s)	809, 1088, 1609
AcuI	CTGAAG	3 cut(s)	408, 1125, 2246
AdeI	CACNNNGTG	1 cut(s)	533
AfiI	CCNNNNNNNGG	5 cut(s)	255, 1256, 1257, 1613, 1906
AflIII	ACRYGT	1 cut(s)	1743
AhlI	ACTAGT	2 cut(s)	319, 725
AjiI	CACGTC	1 cut(s)	1744
AjnI	CCWGG	5 cut(s)	402, 498, 1833, 2022, 2241
Alw21I	GWGCWC	3 cut(s)	529, 1151, 1790
Alw26I	GTCTC	6 cut(s)	593, 1124, 1622, 1722, 2098, 2232
Alw44I	GTGCAC	1 cut(s)	1147
AlwI	GGATC	6 cut(s)	135, 267, 697, 1235, 1525, 1538
Ama87I	CYCGRG	4 cut(s)	596, 1256, 1281, 1706
AoxI	GGCC	1 cut(s)	1132
ApaLI	GTGCAC	1 cut(s)	1147
ApeKI	GCWGC	6 cut(s)	215, 306, 350, 952, 1601, 1646
ApoI	RAATTY	3 cut(s)	809, 1088, 1609
ArsI	GACNNNNNNTTYG	2 cut(s)	2231, 2263
AseI	ATTAAT	1 cut(s)	1503
AspA2I	CCTAGG	1 cut(s)	995
AspLEI	GCGC	1 cut(s)	1013
AspS9I	GGNCC	3 cut(s)	1133, 1399, 1578
AsuC2I	CCSGG	1 cut(s)	2150
AsuHPI	GGTGA	3 cut(s)	185, 2038, 2083
AsuII	TTCGAA	3 cut(s)	1025, 1093, 2250
AsuNHI	GCTAGC	1 cut(s)	1318
AvaI	CYCGRG	4 cut(s)	596, 1256, 1281, 1706
AvaII	GGWCC	2 cut(s)	1399, 1578
AvrII	CCTAGG	1 cut(s)	995
BaeGI	GKGCMC	1 cut(s)	1151
BamHI	GGATCC	1 cut(s)	1530
BanII	GRGCYC	2 cut(s)	1814, 1899
BbsI	GAAGAC	3 cut(s)	328, 414, 1034
Bbv12I	GWGCWC	3 cut(s)	529, 1151, 1790
BbvI	GCAGC	6 cut(s)	202, 318, 337, 939, 1588, 1658
BccI	CCATC	5 cut(s)	260, 1513, 1843, 1998, 2197
BceAI	ACGGC	1 cut(s)	525
BciT130I	CCWGG	5 cut(s)	404, 500, 1835, 2024, 2243
BclI	TGATCA	2 cut(s)	123, 1494
BcnI	CCSGG	1 cut(s)	2150
BcoDI	GTCTC	6 cut(s)	593, 1124, 1622, 1722, 2098, 2232
BcuI	ACTAGT	2 cut(s)	319, 725
BfaI	CTAG	9 cut(s)	36, 263, 320, 647, 726, 996, 1319, 1323, 1683
BfmI	CTRYAG	3 cut(s)	555, 1152, 2058
BfuAI	ACCTGC	1 cut(s)	1337
BglII	AGATCT	1 cut(s)	1986
BisI	GCNGC	6 cut(s)	216, 307, 351, 953, 1602, 1647
BlnI	CCTAGG	1 cut(s)	995
BlpI	GCTNAGC	1 cut(s)	944
BlsI	GCNGC	6 cut(s)	217, 308, 352, 954, 1603, 1648
BmcAI	AGTACT	3 cut(s)	411, 1371, 2052
Bme1390I	CCNGG	6 cut(s)	404, 500, 1835, 2024, 2150, 2243
Bme18I	GGWCC	2 cut(s)	1399, 1578
BmeT110I	CYCGRG	4 cut(s)	596, 1256, 1281, 1706
BmgBI	CACGTC	1 cut(s)	1744
BmgT120I	GGNCC	3 cut(s)	1133, 1399, 1578
BmiI	GGNNCC	3 cut(s)	1401, 1532, 1666
BmrFI	CCNGG	6 cut(s)	404, 500, 1835, 2024, 2150, 2243
BmsI	GCATC	6 cut(s)	164, 226, 526, 736, 1398, 1552
BmtI	GCTAGC	1 cut(s)	1322
BoxI	GACNNNNGTC	1 cut(s)	2144
BpiI	GAAGAC	3 cut(s)	328, 414, 1034
BpmI	CTGGAG	1 cut(s)	2122
Bpu1102I	GCTNAGC	1 cut(s)	944
Bpu14I	TTCGAA	3 cut(s)	1025, 1093, 2250
BpuMI	CCSGG	1 cut(s)	2150
BsaAI	YACGTR	2 cut(s)	274, 1376
BsaBI	GATNNNNATC	1 cut(s)	436
BsaI	GGTCTC	3 cut(s)	1622, 2098, 2232
BsaJI	CCNNGG	9 cut(s)	144, 386, 403, 499, 804, 995, 1255, 1834, 2242
BsaWI	WCCGGW	1 cut(s)	2110
Bsc4I	CCNNNNNNNGG	5 cut(s)	255, 1256, 1257, 1613, 1906
Bse1I	ACTGG	3 cut(s)	102, 301, 2105
Bse3DI	GCAATG	2 cut(s)	288, 1923
Bse8I	GATNNNNATC	1 cut(s)	436
BseBI	CCWGG	5 cut(s)	404, 500, 1835, 2024, 2243
BseDI	CCNNGG	9 cut(s)	144, 386, 403, 499, 804, 995, 1255, 1834, 2242
BseGI	GGATG	7 cut(s)	437, 791, 1332, 1636, 1830, 2009, 2191
BseJI	GATNNNNATC	1 cut(s)	436
BseLI	CCNNNNNNNGG	5 cut(s)	255, 1256, 1257, 1613, 1906
BseMI	GCAATG	2 cut(s)	288, 1923
BseMII	CTCAG	6 cut(s)	449, 714, 725, 1129, 1135, 1376
BseNI	ACTGG	3 cut(s)	102, 301, 2105
BseRI	GAGGAG	1 cut(s)	179
BseSI	GKGCMC	1 cut(s)	1151
BseXI	GCAGC	6 cut(s)	202, 318, 337, 939, 1588, 1658
BsgI	GTGCAG	6 cut(s)	336, 581, 588, 1000, 1665, 2003
Bsh1236I	CGCG	2 cut(s)	146, 1560
Bsh1285I	CGRYCG	2 cut(s)	753, 1204
BshFI	GGCC	1 cut(s)	1134
BsiEI	CGRYCG	2 cut(s)	753, 1204
BsiHKAI	GWGCWC	3 cut(s)	529, 1151, 1790
BsiHKCI	CYCGRG	4 cut(s)	596, 1256, 1281, 1706
BsiSI	CCGG	3 cut(s)	1961, 2111, 2149
BsiWI	CGTACG	1 cut(s)	967
BslFI	GGGAC	4 cut(s)	53, 1013, 1274, 1892
BslI	CCNNNNNNNGG	5 cut(s)	255, 1256, 1257, 1613, 1906
BsmAI	GTCTC	6 cut(s)	593, 1124, 1622, 1722, 2098, 2232
BsmFI	GGGAC	4 cut(s)	53, 1013, 1274, 1892
BsmI	GAATGC	1 cut(s)	1589
BsnI	GGCC	1 cut(s)	1134
Bso31I	GGTCTC	3 cut(s)	1622, 2098, 2232
BsoBI	CYCGRG	4 cut(s)	596, 1256, 1281, 1706
Bsp119I	TTCGAA	3 cut(s)	1025, 1093, 2250
Bsp1286I	GDGCHC	5 cut(s)	529, 1151, 1790, 1814, 1899
Bsp1407I	TGTACA	2 cut(s)	491, 1303
Bsp1720I	GCTNAGC	1 cut(s)	944
Bsp68I	TCGCGA	1 cut(s)	1560
BspACI	CCGC	8 cut(s)	144, 146, 171, 429, 749, 1225, 1534, 1576
BspANI	GGCC	1 cut(s)	1134
BspCNI	CTCAG	6 cut(s)	450, 713, 724, 1128, 1134, 1377
BspFNI	CGCG	2 cut(s)	146, 1560
BspLI	GGNNCC	3 cut(s)	1401, 1532, 1666
BspMI	ACCTGC	1 cut(s)	1337
BspOI	GCTAGC	1 cut(s)	1322
BspPI	GGATC	6 cut(s)	135, 267, 697, 1235, 1525, 1538
BspQI	GCTCTTC	1 cut(s)	1964
BspT104I	TTCGAA	3 cut(s)	1025, 1093, 2250
BspTNI	GGTCTC	3 cut(s)	1622, 2098, 2232
BsrDI	GCAATG	2 cut(s)	288, 1923
BsrGI	TGTACA	2 cut(s)	491, 1303
BsrI	ACTGG	3 cut(s)	102, 301, 2105
BssECI	CCNNGG	9 cut(s)	144, 386, 403, 499, 804, 995, 1255, 1834, 2242
BssT1I	CCWWGG	3 cut(s)	386, 804, 995
Bst2UI	CCWGG	5 cut(s)	404, 500, 1835, 2024, 2243
Bst4CI	ACNGT	4 cut(s)	1153, 1792, 1981, 2055
Bst6I	CTCTTC	3 cut(s)	1758, 1964, 2230
BstAUI	TGTACA	2 cut(s)	491, 1303
BstBAI	YACGTR	2 cut(s)	274, 1376
BstBI	TTCGAA	3 cut(s)	1025, 1093, 2250
BstC8I	GCNNGC	8 cut(s)	348, 950, 1009, 1015, 1320, 1538, 2127, 2131
BstDEI	CTNAG	9 cut(s)	137, 458, 700, 711, 887, 944, 1115, 1121, 1385
BstDSI	CCRYGG	1 cut(s)	144
BstF5I	GGATG	7 cut(s)	437, 791, 1332, 1636, 1830, 2009, 2191
BstFNI	CGCG	2 cut(s)	146, 1560
BstHHI	GCGC	1 cut(s)	1013
BstMAI	GTCTC	6 cut(s)	593, 1124, 1622, 1722, 2098, 2232
BstMCI	CGRYCG	2 cut(s)	753, 1204
BstMWI	GCNNNNNNNGC	8 cut(s)	152, 245, 647, 949, 1131, 1174, 1662, 2014
BstNI	CCWGG	5 cut(s)	404, 500, 1835, 2024, 2243
BstNSI	RCATGY	4 cut(s)	498, 1011, 2084, 2133
BstPAI	GACNNNNGTC	1 cut(s)	2144
BstSCI	CCNGG	6 cut(s)	402, 498, 1833, 2022, 2148, 2241
BstSFI	CTRYAG	3 cut(s)	555, 1152, 2058
BstSLI	GKGCMC	1 cut(s)	1151
BstSNI	TACGTA	1 cut(s)	1376
BstUI	CGCG	2 cut(s)	146, 1560
BstV1I	GCAGC	6 cut(s)	202, 318, 337, 939, 1588, 1658
BstV2I	GAAGAC	3 cut(s)	328, 414, 1034
BstX2I	RGATCY	6 cut(s)	140, 259, 689, 1227, 1530, 1986
BstXI	CCANNNNNNTGG	2 cut(s)	880, 1067
BstYI	RGATCY	6 cut(s)	140, 259, 689, 1227, 1530, 1986
BsuRI	GGCC	1 cut(s)	1134
BtgI	CCRYGG	1 cut(s)	144
BtgZI	GCGATG	1 cut(s)	1575
BtrI	CACGTC	1 cut(s)	1744
BtsCI	GGATG	7 cut(s)	437, 791, 1332, 1636, 1830, 2009, 2191
BtsI	GCAGTG	1 cut(s)	569
BtsIMutI	CAGTG	6 cut(s)	95, 569, 663, 1149, 1788, 1986
BtuMI	TCGCGA	1 cut(s)	1560
BveI	ACCTGC	1 cut(s)	1337
Cac8I	GCNNGC	8 cut(s)	348, 950, 1009, 1015, 1320, 1538, 2127, 2131
CfoI	GCGC	1 cut(s)	1013
Cfr13I	GGNCC	3 cut(s)	1133, 1399, 1578
Cfr42I	CCGCGG	1 cut(s)	147
CspCI	CAANNNNNGTGG	2 cut(s)	31, 66
DdeI	CTNAG	9 cut(s)	137, 458, 700, 711, 887, 944, 1115, 1121, 1385
DraI	TTTAAA	1 cut(s)	67
DraIII	CACNNNGTG	1 cut(s)	533
Eam1104I	CTCTTC	3 cut(s)	1758, 1964, 2230
EarI	CTCTTC	3 cut(s)	1758, 1964, 2230
EciI	GGCGGA	1 cut(s)	444
Eco105I	TACGTA	1 cut(s)	1376
Eco130I	CCWWGG	3 cut(s)	386, 804, 995
Eco24I	GRGCYC	2 cut(s)	1814, 1899
Eco31I	GGTCTC	3 cut(s)	1622, 2098, 2232
Eco47I	GGWCC	2 cut(s)	1399, 1578
Eco57I	CTGAAG	3 cut(s)	408, 1125, 2246
Eco88I	CYCGRG	4 cut(s)	596, 1256, 1281, 1706
EcoO109I	RGGNCCY	1 cut(s)	1133
EcoRI	GAATTC	1 cut(s)	809
EcoRII	CCWGG	5 cut(s)	402, 498, 1833, 2022, 2241
EcoT14I	CCWWGG	3 cut(s)	386, 804, 995
EcoT22I	ATGCAT	3 cut(s)	290, 541, 1591
EcoT38I	GRGCYC	2 cut(s)	1814, 1899
ErhI	CCWWGG	3 cut(s)	386, 804, 995
FalI	AAGNNNNNCTT	2 cut(s)	1122, 1154
FaqI	GGGAC	4 cut(s)	53, 1013, 1274, 1892
FauI	CCCGC	1 cut(s)	139
FauNDI	CATATG	2 cut(s)	637, 1585
FbaI	TGATCA	2 cut(s)	123, 1494
Fnu4HI	GCNGC	6 cut(s)	216, 307, 351, 953, 1602, 1647
FokI	GGATG	7 cut(s)	424, 798, 1339, 1643, 1817, 2016, 2198
FriOI	GRGCYC	2 cut(s)	1814, 1899
Fsp4HI	GCNGC	6 cut(s)	216, 307, 351, 953, 1602, 1647
FspBI	CTAG	9 cut(s)	36, 263, 320, 647, 726, 996, 1319, 1323, 1683
GlaI	GCGC	1 cut(s)	1012
GluI	GCNGC	6 cut(s)	216, 307, 351, 953, 1602, 1647
GsuI	CTGGAG	1 cut(s)	2122
HaeIII	GGCC	1 cut(s)	1134
HapII	CCGG	3 cut(s)	1961, 2111, 2149
HhaI	GCGC	1 cut(s)	1013
Hin6I	GCGC	1 cut(s)	1011
HinP1I	GCGC	1 cut(s)	1011
HincII	GTYRAC	4 cut(s)	317, 616, 1047, 2139
HindII	GTYRAC	4 cut(s)	317, 616, 1047, 2139
HindIII	AAGCTT	3 cut(s)	359, 1414, 2258
HpaII	CCGG	3 cut(s)	1961, 2111, 2149
HphI	GGTGA	3 cut(s)	185, 2038, 2083
Hpy166II	GTNNAC	6 cut(s)	317, 616, 1047, 1149, 1921, 2139
Hpy188III	TCNNGA	7 cut(s)	263, 596, 598, 984, 1559, 2174, 2215
Hpy8I	GTNNAC	6 cut(s)	317, 616, 1047, 1149, 1921, 2139
Hpy99I	CGWCG	2 cut(s)	836, 1202
HpyCH4III	ACNGT	4 cut(s)	1153, 1792, 1981, 2055
HpyCH4IV	ACGT	7 cut(s)	273, 467, 834, 1375, 1743, 1881, 1888
HpyF10VI	GCNNNNNNNGC	8 cut(s)	152, 245, 647, 949, 1131, 1174, 1662, 2014
HpyF3I	CTNAG	9 cut(s)	137, 458, 700, 711, 887, 944, 1115, 1121, 1385
HpySE526I	ACGT	7 cut(s)	273, 467, 834, 1375, 1743, 1881, 1888
HspAI	GCGC	1 cut(s)	1011
Ksp22I	TGATCA	2 cut(s)	123, 1494
KspI	CCGCGG	1 cut(s)	147
LguI	GCTCTTC	1 cut(s)	1964
LmnI	GCTCC	7 cut(s)	454, 524, 1315, 1609, 1670, 1785, 2077
Lsp1109I	GCAGC	6 cut(s)	202, 318, 337, 939, 1588, 1658
LweI	GCATC	6 cut(s)	164, 226, 526, 736, 1398, 1552
MaeI	CTAG	9 cut(s)	36, 263, 320, 647, 726, 996, 1319, 1323, 1683
MaeII	ACGT	7 cut(s)	273, 467, 834, 1375, 1743, 1881, 1888
MaeIII	GTNAC	4 cut(s)	56, 908, 1160, 2026
MfeI	CAATTG	1 cut(s)	1426
MflI	RGATCY	6 cut(s)	140, 259, 689, 1227, 1530, 1986
MhlI	GDGCHC	5 cut(s)	529, 1151, 1790, 1814, 1899
MluCI	AATT	8 cut(s)	591, 809, 855, 1088, 1426, 1504, 1523, 1609
MlyI	GAGTC	5 cut(s)	28, 479, 910, 981, 1904
MmeI	TCCRAC	1 cut(s)	1530
Mph1103I	ATGCAT	3 cut(s)	290, 541, 1591
MseI	TTAA	9 cut(s)	66, 102, 114, 470, 590, 696, 1076, 1503, 1821
MslI	CAYNNNNRTG	3 cut(s)	878, 1571, 1998
MspA1I	CMGCKG	1 cut(s)	146
MspI	CCGG	3 cut(s)	1961, 2111, 2149
MspR9I	CCNGG	6 cut(s)	404, 500, 1835, 2024, 2150, 2243
MunI	CAATTG	1 cut(s)	1426
Mva1269I	GAATGC	1 cut(s)	1589
MvaI	CCWGG	5 cut(s)	404, 500, 1835, 2024, 2243
MvnI	CGCG	2 cut(s)	146, 1560
MwoI	GCNNNNNNNGC	8 cut(s)	152, 245, 647, 949, 1131, 1174, 1662, 2014
NciI	CCSGG	1 cut(s)	2150
NdeI	CATATG	2 cut(s)	637, 1585
NheI	GCTAGC	1 cut(s)	1318
NlaIV	GGNNCC	3 cut(s)	1401, 1532, 1666
NmuCI	GTSAC	2 cut(s)	1160, 2026
NruI	TCGCGA	1 cut(s)	1560
NsiI	ATGCAT	3 cut(s)	290, 541, 1591
NspI	RCATGY	4 cut(s)	498, 1011, 2084, 2133
NspV	TTCGAA	3 cut(s)	1025, 1093, 2250
PaeI	GCATGC	2 cut(s)	1011, 2133
PaeR7I	CTCGAG	2 cut(s)	596, 1706
PciSI	GCTCTTC	1 cut(s)	1964
PcsI	WCGNNNNNNNCGW	3 cut(s)	1197, 1403, 1853
PctI	GAATGC	1 cut(s)	1589
PfeI	GAWTC	6 cut(s)	382, 446, 740, 773, 2177, 2247
Pfl23II	CGTACG	1 cut(s)	967
PfoI	TCCNGGA	1 cut(s)	2148
PkrI	GCNGC	6 cut(s)	217, 308, 352, 954, 1603, 1648
Ple19I	CGATCG	1 cut(s)	1204
PleI	GAGTC	5 cut(s)	27, 479, 910, 981, 1904
PpsI	GAGTC	5 cut(s)	27, 479, 910, 981, 1904
Ppu21I	YACGTR	2 cut(s)	274, 1376
PshAI	GACNNNNGTC	1 cut(s)	2144
PshBI	ATTAAT	1 cut(s)	1503
Psp1406I	AACGTT	1 cut(s)	467
Psp6I	CCWGG	5 cut(s)	402, 498, 1833, 2022, 2241
PspGI	CCWGG	5 cut(s)	402, 498, 1833, 2022, 2241
PspLI	CGTACG	1 cut(s)	967
PspN4I	GGNNCC	3 cut(s)	1401, 1532, 1666
PspPI	GGNCC	3 cut(s)	1133, 1399, 1578
PspXI	VCTCGAGB	1 cut(s)	1706
PsuI	RGATCY	6 cut(s)	140, 259, 689, 1227, 1530, 1986
PvuI	CGATCG	1 cut(s)	1204
RruI	TCGCGA	1 cut(s)	1560
RseI	CAYNNNNRTG	3 cut(s)	878, 1571, 1998
SacII	CCGCGG	1 cut(s)	147
SapI	GCTCTTC	1 cut(s)	1964
SaqAI	TTAA	9 cut(s)	66, 102, 114, 470, 590, 696, 1076, 1503, 1821
SatI	GCNGC	6 cut(s)	216, 307, 351, 953, 1602, 1647
Sau96I	GGNCC	3 cut(s)	1133, 1399, 1578
ScaI	AGTACT	3 cut(s)	411, 1371, 2052
SchI	GAGTC	5 cut(s)	28, 479, 910, 981, 1904
ScrFI	CCNGG	6 cut(s)	404, 500, 1835, 2024, 2150, 2243
SduI	GDGCHC	5 cut(s)	529, 1151, 1790, 1814, 1899
SfaNI	GCATC	6 cut(s)	164, 226, 526, 736, 1398, 1552
SfcI	CTRYAG	3 cut(s)	555, 1152, 2058
Sfr274I	CTCGAG	2 cut(s)	596, 1706
Sfr303I	CCGCGG	1 cut(s)	147
SfuI	TTCGAA	3 cut(s)	1025, 1093, 2250
SgrBI	CCGCGG	1 cut(s)	147
SinI	GGWCC	2 cut(s)	1399, 1578
SlaI	CTCGAG	2 cut(s)	596, 1706
SmiMI	CAYNNNNRTG	3 cut(s)	878, 1571, 1998
SmlI	CTYRAG	2 cut(s)	596, 1706
SmoI	CTYRAG	2 cut(s)	596, 1706
SnaBI	TACGTA	1 cut(s)	1376
SpeI	ACTAGT	2 cut(s)	319, 725
SphI	GCATGC	2 cut(s)	1011, 2133
Sse9I	AATT	8 cut(s)	591, 809, 855, 1088, 1426, 1504, 1523, 1609
SsiI	CCGC	8 cut(s)	144, 146, 171, 429, 749, 1225, 1534, 1576
SspMI	CTAG	9 cut(s)	36, 263, 320, 647, 726, 996, 1319, 1323, 1683
StyD4I	CCNGG	6 cut(s)	402, 498, 1833, 2022, 2148, 2241
StyI	CCWWGG	3 cut(s)	386, 804, 995
TaaI	ACNGT	4 cut(s)	1153, 1792, 1981, 2055
TaiI	ACGT	7 cut(s)	276, 470, 837, 1378, 1746, 1884, 1891
TasI	AATT	8 cut(s)	591, 809, 855, 1088, 1426, 1504, 1523, 1609
TatI	WGTACW	5 cut(s)	409, 491, 1303, 1369, 2050
TfiI	GAWTC	6 cut(s)	382, 446, 740, 773, 2177, 2247
Tru1I	TTAA	9 cut(s)	66, 102, 114, 470, 590, 696, 1076, 1503, 1821
Tru9I	TTAA	9 cut(s)	66, 102, 114, 470, 590, 696, 1076, 1503, 1821
TscAI	CASTG	6 cut(s)	102, 569, 670, 1156, 1795, 1986
TseFI	GTSAC	2 cut(s)	1160, 2026
TseI	GCWGC	6 cut(s)	215, 306, 350, 952, 1601, 1646
Tsp45I	GTSAC	2 cut(s)	1160, 2026
TspDTI	ATGAA	6 cut(s)	17, 624, 753, 786, 795, 1287
TspGWI	ACGGA	5 cut(s)	686, 734, 1543, 1657, 1922
TspRI	CASTG	6 cut(s)	102, 569, 670, 1156, 1795, 1986
VneI	GTGCAC	1 cut(s)	1147
VpaK11BI	GGWCC	2 cut(s)	1399, 1578
VspI	ATTAAT	1 cut(s)	1503
XapI	RAATTY	3 cut(s)	809, 1088, 1609
XbaI	TCTAGA	1 cut(s)	262
XceI	RCATGY	4 cut(s)	498, 1011, 2084, 2133
XcmI	CCANNNNNNNNNTGG	2 cut(s)	410, 1516
XhoI	CTCGAG	2 cut(s)	596, 1706
XmaJI	CCTAGG	1 cut(s)	995
XspI	CTAG	9 cut(s)	36, 263, 320, 647, 726, 996, 1319, 1323, 1683
ZrmI	AGTACT	3 cut(s)	411, 1371, 2052
Zsp2I	ATGCAT	3 cut(s)	290, 541, 1591

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.