MD11G1263700.v1.1

Rab3 gtpase-activating protein non-catalytic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
N/A
Physical Location & Seq
Reverse (-)
37883572 .. 37886274
2703 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1263700.v1.1.491

Sequence Viewer

Length: 543 bp
ATGGCGAAGCGAACTCATACGACGGAGCTGGGCAGCATCGCCTGCGAGGAGCTCGGCGAGCTCGGCGCGGGAAAGGAAGGCTGGTTCGACAACCCCAACCTCCTCTGCGCCCTGGACACCCAGTCGCTCGCTCTCGCCAACCGCTCCGTTATTGTGTTTCTCGGGTGGGCCGACTCCGACGGGACCCCCCTCAAAATTCGACCCGAATTGTCCCCGATCGAGGCGGAGTTCGTCACGGCGGTGGAGTGGTTGGTGTTTGACGAGGTAAGGGTGGTTGTGGCCGGGACTTCGTCTGGGTATCTCCTATTTTTCTCTTTGGATGGAGATTTGATTCATAAGCAGATTTTGCATGTTGGAAAGGGAAATGGAGAAATGGGGTTTCCGTGCACGATAAGGAGGATGAAGGTGGGGTTTCGGTCTGGATTTAGGTTAGGGTGCAGGATAAGGAGGAGGAAGAGGAGGGTGGGGTTTAGGATGGATGTGCAGAGGTTGGGTAGGTGCAGAGAAGAGAAGGGAGGGGGGTCGGGGTGGGGAAGACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RAB3GAP2_N

Protein Analysis

181

Amino Acids

20.0

Weight (kDa)

9.33

Isoelectric Point (pI)

49.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RAB3GAP2_N PF14655 28 - 118 3.9e-17 Rab3 GTPase-activating protein regulatory subunit N-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 144
AccII CGCG 1 cut(s) 68
AciI CCGC 4 cut(s) 68, 142, 224, 239
AcoI YGGCCR 1 cut(s) 279
AcsI RAATTY 1 cut(s) 195
AfiI CCNNNNNNNGG 1 cut(s) 220
AhdI GACNNNNNGTC 1 cut(s) 121
AjnI CCWGG 1 cut(s) 111
AleI CACNNNNGTG 1 cut(s) 239
AloI GAACNNNNNNTCC 2 cut(s) 68, 100
AluBI AGCT 3 cut(s) 28, 52, 61
AluI AGCT 3 cut(s) 28, 52, 61
Alw21I GWGCWC 3 cut(s) 54, 63, 389
Alw44I GTGCAC 1 cut(s) 385
Ama87I CYCGRG 1 cut(s) 161
AoxI GGCC 2 cut(s) 168, 279
ApaLI GTGCAC 1 cut(s) 385
ApeKI GCWGC 1 cut(s) 33
ApoI RAATTY 1 cut(s) 195
AspLEI GCGC 2 cut(s) 68, 110
AspS9I GGNCC 2 cut(s) 168, 183
AsuC2I CCSGG 1 cut(s) 283
AvaI CYCGRG 1 cut(s) 161
AvaII GGWCC 1 cut(s) 183
BaeGI GKGCMC 1 cut(s) 389
BanII GRGCYC 2 cut(s) 54, 63
Bbv12I GWGCWC 3 cut(s) 54, 63, 389
BbvI GCAGC 1 cut(s) 45
BccI CCATC 2 cut(s) 314, 469
BceAI ACGGC 1 cut(s) 252
BciT130I CCWGG 1 cut(s) 113
BcnI CCSGG 1 cut(s) 283
BisI GCNGC 1 cut(s) 34
BlsI GCNGC 1 cut(s) 35
Bme1390I CCNGG 2 cut(s) 113, 283
Bme18I GGWCC 1 cut(s) 183
BmeRI GACNNNNNGTC 1 cut(s) 121
BmeT110I CYCGRG 1 cut(s) 161
BmgT120I GGNCC 2 cut(s) 168, 183
BmiI GGNNCC 2 cut(s) 184, 185
BmrFI CCNGG 2 cut(s) 113, 283
BmrI ACTGGG 1 cut(s) 115
BmsI GCATC 1 cut(s) 45
BmuI ACTGGG 1 cut(s) 115
BpuMI CCSGG 1 cut(s) 283
BsaJI CCNNGG 1 cut(s) 111
Bsc4I CCNNNNNNNGG 1 cut(s) 220
Bse1I ACTGG 1 cut(s) 121
BseBI CCWGG 1 cut(s) 113
BseDI CCNNGG 1 cut(s) 111
BseGI GGATG 4 cut(s) 325, 405, 480, 484
BseLI CCNNNNNNNGG 1 cut(s) 220
BseNI ACTGG 1 cut(s) 121
BseRI GAGGAG 4 cut(s) 62, 92, 463, 472
BseSI GKGCMC 1 cut(s) 389
BseXI GCAGC 1 cut(s) 45
BseYI CCCAGC 1 cut(s) 28
BsgI GTGCAG 3 cut(s) 457, 503, 520
Bsh1236I CGCG 1 cut(s) 68
Bsh1285I CGRYCG 1 cut(s) 219
BshFI GGCC 2 cut(s) 170, 281
BsiEI CGRYCG 1 cut(s) 219
BsiHKAI GWGCWC 3 cut(s) 54, 63, 389
BsiHKCI CYCGRG 1 cut(s) 161
BsiSI CCGG 1 cut(s) 282
BslFI GGGAC 3 cut(s) 196, 196, 298
BslI CCNNNNNNNGG 1 cut(s) 220
BsmFI GGGAC 3 cut(s) 196, 196, 298
BsnI GGCC 2 cut(s) 170, 281
BsoBI CYCGRG 1 cut(s) 161
Bsp1286I GDGCHC 3 cut(s) 54, 63, 389
Bsp143I GATC 1 cut(s) 216
BspACI CCGC 4 cut(s) 68, 142, 224, 239
BspANI GGCC 2 cut(s) 170, 281
BspFNI CGCG 1 cut(s) 68
BspLI GGNNCC 2 cut(s) 184, 185
BsrBI CCGCTC 1 cut(s) 144
BsrI ACTGG 1 cut(s) 121
BssECI CCNNGG 1 cut(s) 111
BssMI GATC 1 cut(s) 216
Bst2UI CCWGG 1 cut(s) 113
Bst6I CTCTTC 2 cut(s) 449, 501
BstAPI GCANNNNNTGC 2 cut(s) 42, 346
BstC8I GCNNGC 3 cut(s) 43, 59, 129
BstF5I GGATG 4 cut(s) 325, 405, 480, 484
BstFNI CGCG 1 cut(s) 68
BstHHI GCGC 2 cut(s) 68, 110
BstKTI GATC 1 cut(s) 219
BstMBI GATC 1 cut(s) 216
BstMCI CGRYCG 1 cut(s) 219
BstMWI GCNNNNNNNGC 4 cut(s) 42, 58, 63, 346
BstNI CCWGG 1 cut(s) 113
BstNSI RCATGY 1 cut(s) 353
BstSCI CCNGG 2 cut(s) 111, 281
BstSLI GKGCMC 1 cut(s) 389
BstUI CGCG 1 cut(s) 68
BstV1I GCAGC 1 cut(s) 45
BsuRI GGCC 2 cut(s) 170, 281
BtgZI GCGATG 1 cut(s) 22
BtsCI GGATG 4 cut(s) 325, 405, 480, 484
Cac8I GCNNGC 3 cut(s) 43, 59, 129
CfoI GCGC 2 cut(s) 68, 110
Cfr13I GGNCC 2 cut(s) 168, 183
CviAII CATG 1 cut(s) 350
CviJI RGCY 6 cut(s) 28, 52, 61, 81, 170, 281
CviKI_1 RGCY 6 cut(s) 28, 52, 61, 81, 170, 281
DpnI GATC 1 cut(s) 218
DpnII GATC 1 cut(s) 216
DriI GACNNNNNGTC 1 cut(s) 121
EaeI YGGCCR 1 cut(s) 279
Eam1104I CTCTTC 2 cut(s) 449, 501
Eam1105I GACNNNNNGTC 1 cut(s) 121
EarI CTCTTC 2 cut(s) 449, 501
EciI GGCGGA 1 cut(s) 239
Ecl136II GAGCTC 2 cut(s) 52, 61
Eco24I GRGCYC 2 cut(s) 54, 63
Eco47I GGWCC 1 cut(s) 183
Eco53kI GAGCTC 2 cut(s) 52, 61
Eco88I CYCGRG 1 cut(s) 161
EcoICRI GAGCTC 2 cut(s) 52, 61
EcoO109I RGGNCCY 1 cut(s) 183
EcoRII CCWGG 1 cut(s) 111
EcoT38I GRGCYC 2 cut(s) 54, 63
FaeI CATG 1 cut(s) 353
FaiI YATR 3 cut(s) 18, 336, 351
FaqI GGGAC 3 cut(s) 196, 196, 298
FatI CATG 1 cut(s) 349
FauI CCCGC 1 cut(s) 61
Fnu4HI GCNGC 1 cut(s) 34
FokI GGATG 4 cut(s) 332, 412, 487, 491
FriOI GRGCYC 2 cut(s) 54, 63
Fsp4HI GCNGC 1 cut(s) 34
GlaI GCGC 2 cut(s) 67, 109
GluI GCNGC 1 cut(s) 34
GsaI CCCAGC 1 cut(s) 32
HaeIII GGCC 2 cut(s) 170, 281
HapII CCGG 1 cut(s) 282
HhaI GCGC 2 cut(s) 68, 110
Hin1II CATG 1 cut(s) 353
Hin6I GCGC 2 cut(s) 66, 108
HinP1I GCGC 2 cut(s) 66, 108
HinfI GANTC 2 cut(s) 173, 331
HpaII CCGG 1 cut(s) 282
Hpy166II GTNNAC 1 cut(s) 387
Hpy188I TCNGA 1 cut(s) 178
Hpy188III TCNNGA 1 cut(s) 420
Hpy8I GTNNAC 1 cut(s) 387
Hpy99I CGWCG 2 cut(s) 25, 182
HpyAV CCTTC 3 cut(s) 71, 397, 505
HpyCH4V TGCA 5 cut(s) 349, 387, 438, 484, 501
HpyF10VI GCNNNNNNNGC 4 cut(s) 42, 58, 63, 346
Hsp92II CATG 1 cut(s) 353
HspAI GCGC 2 cut(s) 66, 108
KflI GGGWCCC 1 cut(s) 183
Kzo9I GATC 1 cut(s) 216
LmnI GCTCC 3 cut(s) 25, 49, 149
Lsp1109I GCAGC 1 cut(s) 45
LweI GCATC 1 cut(s) 45
MaeIII GTNAC 1 cut(s) 232
MalI GATC 1 cut(s) 218
MbiI CCGCTC 1 cut(s) 144
MboI GATC 1 cut(s) 216
MboII GAAGA 2 cut(s) 466, 518
MhlI GDGCHC 3 cut(s) 54, 63, 389
MluCI AATT 2 cut(s) 195, 206
MlyI GAGTC 1 cut(s) 167
MmeI TCCRAC 2 cut(s) 201, 334
MslI CAYNNNNRTG 1 cut(s) 239
MspI CCGG 1 cut(s) 282
MspR9I CCNGG 2 cut(s) 113, 283
MvaI CCWGG 1 cut(s) 113
MvnI CGCG 1 cut(s) 68
MwoI GCNNNNNNNGC 4 cut(s) 42, 58, 63, 346
NciI CCSGG 1 cut(s) 283
NdeII GATC 1 cut(s) 216
NlaIII CATG 1 cut(s) 353
NlaIV GGNNCC 2 cut(s) 184, 185
NmeAIII GCCGAG 2 cut(s) 33, 42
NmuCI GTSAC 1 cut(s) 232
NspI RCATGY 1 cut(s) 353
OliI CACNNNNGTG 1 cut(s) 239
PcsI WCGNNNNNNNCGW 1 cut(s) 168
PfeI GAWTC 1 cut(s) 331
PflFI GACNNNGTC 1 cut(s) 289
PkrI GCNGC 1 cut(s) 35
Ple19I CGATCG 1 cut(s) 219
PleI GAGTC 1 cut(s) 167
PpsI GAGTC 1 cut(s) 167
PpuMI RGGWCCY 1 cut(s) 183
Psp124BI GAGCTC 2 cut(s) 54, 63
Psp5II RGGWCCY 1 cut(s) 183
Psp6I CCWGG 1 cut(s) 111
PspFI CCCAGC 1 cut(s) 28
PspGI CCWGG 1 cut(s) 111
PspN4I GGNNCC 2 cut(s) 184, 185
PspPI GGNCC 2 cut(s) 168, 183
PspPPI RGGWCCY 1 cut(s) 183
PsyI GACNNNGTC 1 cut(s) 289
PvuI CGATCG 1 cut(s) 219
RseI CAYNNNNRTG 1 cut(s) 239
SacI GAGCTC 2 cut(s) 54, 63
SatI GCNGC 1 cut(s) 34
Sau3AI GATC 1 cut(s) 216
Sau96I GGNCC 2 cut(s) 168, 183
SchI GAGTC 1 cut(s) 167
ScrFI CCNGG 2 cut(s) 113, 283
SduI GDGCHC 3 cut(s) 54, 63, 389
SetI ASST 9 cut(s) 30, 54, 63, 102, 267, 408, 431, 491, 500
SfaNI GCATC 1 cut(s) 45
SinI GGWCC 1 cut(s) 183
SmiMI CAYNNNNRTG 1 cut(s) 239
Sse9I AATT 2 cut(s) 195, 206
SsiI CCGC 4 cut(s) 68, 142, 224, 239
SstI GAGCTC 2 cut(s) 54, 63
StyD4I CCNGG 2 cut(s) 111, 281
TaqI TCGA 3 cut(s) 87, 199, 219
TaqII GACCGA 1 cut(s) 405
TasI AATT 2 cut(s) 195, 206
TfiI GAWTC 1 cut(s) 331
TseFI GTSAC 1 cut(s) 232
TseI GCWGC 1 cut(s) 33
Tsp45I GTSAC 1 cut(s) 232
TspDTI ATGAA 2 cut(s) 323, 416
TspGWI ACGGA 3 cut(s) 38, 136, 372
Tth111I GACNNNGTC 1 cut(s) 289
VneI GTGCAC 1 cut(s) 385
VpaK11BI GGWCC 1 cut(s) 183
XapI RAATTY 1 cut(s) 195
XceI RCATGY 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.