MD12G1026300.v1.1
HSP70 Family

Belongs to the heat shock protein 70 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
N/A
Physical Location & Seq
Reverse (-)
2911238 .. 2914912
3675 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1026300.v1.1.491

Sequence Viewer

Length: 1782 bp
ATGAGCTGCAACAGTTACTGTTGGAACAGGAAGAGAAAATACATCTTGATGAACAACGGAGTACTGCCATTAGGCAACCCATACAGCTGCCTCCACCGCGCATTATTTCACCTACACCACAGCGTATTATTTCTCCTCCCATCTTCTTTTGCTGGCAATATTATTTCATCTGCACCACCACGTATTAATAATAATATTTCCGATCATCTATTTCCCTCTCATGGCTGCCGCGAAGAAAACCATGCAGTTGGGATTGATCTGGGAACAACGCATTCCCGTGTAGCCGTGTGGCACAAAGATCATGTAGAAATCATACTGAACGATCAGGGCAACAGGAAGACTCCATCTTATGTTGCATTCTCTGAAACTGATGAGAGGTTGGTAGGTGACCAAGCATTTAATCAAGTCGTGAGAAACAGCGCCAACTCAATCTATGTTCAAGGATGCCAAAGGCGGTTCGCGATAGCAGATACAAAGCGGTTACTTGGTAGGAAATTCAGTGACACCTTTGTTCAAAGTCATGTCAATCTGTGGCCATTCAAGGTCATTGAAGGTCCAGCTGACAACCCAATGATTGTGGTTACCCACAATGGCCAAGAGAAACGAGTTTCTATTGAAGAAATCACATCCATGGTTCTGGAAAAGATGCGGAAGATTGCTGATACCTACCTGGGCTCAACTGTGAAAAATGCAGTTATCACTGTCCCCGCTTACTTCAATGACTCACAGCGTCAGGCTACAAAAGATGCTGGTGTTTCTGCTGGCTTAAATGTGATGTGCATTATTAACGAACCAAGTGCTGCTGCCATTGCTTATGGCCTTCACAAGAAGGCCGGTTGGACTCGCATGAGAAATGTGTTGATATTTGATTTGGGTGATGGTACTTTAGATGTGTCATTGCTTACCATGACTAGTTCTGGTGATTTTCAAGTCAAGGCAACGGTTGGAGACACTCACCTTGGGGGTGAAGACTTTGATAACAGAATGGTCAACTATTGTGTTGAGGAATTCAAGAGCAAGTATAAATTGGATGTTAGTAGAAACTTTAGAGCTCTTAGGAGGTTAAAAAATCAATGTGAGAAGGCAAAAAAGGGACTCTCATTTGAATCTGATATTGAAATTGAAATTGACTGTTTGTGCGAGAATACGCGTACTGATTTCACCATAATTATTATTCGTGCTCAATTTGAACACCTAAACTTGGATTTATTCATGAAGTGTATGGAGCCTGTTGAGAAGTGTTTGAGGGAGGCTAACATGAACATAAGCAGTGTGGATGACGTTGTTCTTGCTGGTGGCTCTTCTAGAATTCCAAAGGTGCAACAGCTACTACAGGAGGTTTTCAAGGGGAAGGAGCTGTGCAAGGGCATTAATCCAGATGAGGCTGTGGCATATGGCGCCGCTATTATGATTCAAGTTTTGGATTTGATTCCAAAATGCTCTAGAATTCCTGTGACGAACGTATTATATCTTTCTACCGTTAAGGACAACCAAGACACCATCGATTTCCGCATATATGAGGGCGAGAGTAGCATACCGGAGAATAGTTACTTTTTGGGTGAATGTAGCCTCCGGGACATTCCTCCAACACGAGAAATTTTTAGTTGTGACGGGAACACAAATGCACCCACTAAAGCTCATAAGTTTGATGTTTGCTTTAACATTGATGCTGATGGTATCTTGAGTGTGTTCGTTCAGGATAAGTCCACTGGCCACAAGAATAAGATCACAATCAACCGTGGCATACATAGAACAATTCACATTACATTGAACTGTGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko04010 ko04141 ko04144 ko04213 ko04612 ko04915 ko05134 ko05145 ko05162 ko05164 ko05169 map03040 map04010 map04141 map04144 map04213 map04612 map04915 map05134 map05145 map05162 map05164 map05169
Pfam Domains
Protein Families
HSP70

Protein Analysis

594

Amino Acids

66.42

Weight (kDa)

7.25

Isoelectric Point (pI)

38.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 82 - 474 4.1e-146 Hsp70 protein
MreB_Mbl PF06723 208 - 475 7.5e-15 MreB/Mbl protein
HSP70 PF00012 476 - 580 7.1e-11 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1397
AccII CGCG 4 cut(s) 99, 231, 461, 1150
AciI CCGC 8 cut(s) 97, 229, 454, 478, 649, 708, 1401, 1510
AcoI YGGCCR 3 cut(s) 533, 592, 1711
AcsI RAATTY 5 cut(s) 494, 1007, 1308, 1446, 1596
AcyI GRCGYC 1 cut(s) 1398
AfaI GTAC 3 cut(s) 63, 883, 1153
AfiI CCNNNNNNNGG 2 cut(s) 221, 1201
AflIII ACRYGT 1 cut(s) 1148
AhlI ACTAGT 1 cut(s) 911
AjnI CCWGG 1 cut(s) 669
AluBI AGCT 7 cut(s) 6, 87, 560, 1052, 1327, 1357, 1637
AluI AGCT 7 cut(s) 6, 87, 560, 1052, 1327, 1357, 1637
Alw21I GWGCWC 2 cut(s) 1054, 1183
Alw26I GTCTC 1 cut(s) 942
AlwNI CAGNNNCTG 1 cut(s) 18
AoxI GGCC 5 cut(s) 533, 592, 817, 831, 1711
ApeKI GCWGC 5 cut(s) 6, 87, 225, 800, 803
ApoI RAATTY 5 cut(s) 494, 1007, 1308, 1446, 1596
AseI ATTAAT 2 cut(s) 186, 1371
AspLEI GCGC 3 cut(s) 101, 422, 1400
AspS9I GGNCC 1 cut(s) 554
AsuC2I CCSGG 1 cut(s) 1574
AsuHPI GGTGA 8 cut(s) 101, 398, 887, 932, 947, 977, 1153, 1571
AvaII GGWCC 1 cut(s) 554
BalI TGGCCA 3 cut(s) 535, 594, 1713
BanI GGYRCC 1 cut(s) 1397
BanII GRGCYC 2 cut(s) 677, 1054
BarI GAAGNNNNNNTAC 2 cut(s) 23, 55
BauI CACGAG 1 cut(s) 1590
BbsI GAAGAC 2 cut(s) 344, 975
Bbv12I GWGCWC 2 cut(s) 1054, 1183
BbvI GCAGC 4 cut(s) 74, 212, 787, 790
BccI CCATC 5 cut(s) 148, 352, 872, 1508, 1667
BceAI ACGGC 1 cut(s) 269
BcgI CGANNNNNNTGC 2 cut(s) 779, 813
BciT130I CCWGG 1 cut(s) 671
BcnI CCSGG 1 cut(s) 1574
BcoDI GTCTC 1 cut(s) 942
BcuI ACTAGT 1 cut(s) 911
BfaI CTAG 3 cut(s) 912, 1305, 1443
BfmI CTRYAG 1 cut(s) 1331
BfoI RGCGCY 2 cut(s) 423, 1401
BisI GCNGC 7 cut(s) 7, 88, 226, 229, 801, 804, 1401
BlsI GCNGC 7 cut(s) 8, 89, 227, 230, 802, 805, 1402
BmcAI AGTACT 1 cut(s) 63
Bme1390I CCNGG 2 cut(s) 671, 1574
Bme18I GGWCC 1 cut(s) 554
BmgT120I GGNCC 1 cut(s) 554
BmiI GGNNCC 2 cut(s) 1227, 1399
BmrFI CCNGG 2 cut(s) 671, 1574
BmsI GCATC 4 cut(s) 434, 636, 736, 1657
BpiI GAAGAC 2 cut(s) 344, 975
BpuEI CTTGAG 1 cut(s) 1702
BpuMI CCSGG 1 cut(s) 1574
Bsa29I ATCGAT 1 cut(s) 1503
BsaAI YACGTR 1 cut(s) 182
BsaBI GATNNNNATC 1 cut(s) 1730
BsaHI GRCGYC 1 cut(s) 1398
BsaJI CCNNGG 4 cut(s) 630, 670, 958, 1738
BsaWI WCCGGW 1 cut(s) 1537
Bsc4I CCNNNNNNNGG 2 cut(s) 221, 1201
Bse118I RCCGGY 1 cut(s) 833
Bse1I ACTGG 1 cut(s) 1714
Bse3DI GCAATG 2 cut(s) 807, 896
Bse8I GATNNNNATC 1 cut(s) 1730
BseBI CCWGG 1 cut(s) 671
BseCI ATCGAT 1 cut(s) 1503
BseDI CCNNGG 4 cut(s) 630, 670, 958, 1738
BseGI GGATG 4 cut(s) 449, 626, 1036, 1282
BseJI GATNNNNATC 1 cut(s) 1730
BseLI CCNNNNNNNGG 2 cut(s) 221, 1201
BseMI GCAATG 2 cut(s) 807, 896
BseNI ACTGG 1 cut(s) 1714
BseRI GAGGAG 1 cut(s) 125
BseXI GCAGC 4 cut(s) 74, 212, 787, 790
BsgI GTGCAG 1 cut(s) 156
Bsh1236I CGCG 4 cut(s) 99, 231, 461, 1150
BshFI GGCC 5 cut(s) 535, 594, 819, 833, 1713
BshNI GGYRCC 1 cut(s) 1397
BshVI ATCGAT 1 cut(s) 1503
BsiHKAI GWGCWC 2 cut(s) 1054, 1183
BsiSI CCGG 3 cut(s) 834, 1538, 1573
BslFI GGGAC 3 cut(s) 689, 1107, 1589
BslI CCNNNNNNNGG 2 cut(s) 221, 1201
BsmAI GTCTC 1 cut(s) 942
BsmFI GGGAC 3 cut(s) 689, 1107, 1589
BsmI GAATGC 2 cut(s) 271, 356
BsnI GGCC 5 cut(s) 535, 594, 819, 833, 1713
Bsp1286I GDGCHC 3 cut(s) 677, 1054, 1183
Bsp143I GATC 5 cut(s) 202, 256, 298, 322, 1725
Bsp19I CCATGG 1 cut(s) 630
Bsp68I TCGCGA 1 cut(s) 461
BspACI CCGC 8 cut(s) 97, 229, 454, 478, 649, 708, 1401, 1510
BspANI GGCC 5 cut(s) 535, 594, 819, 833, 1713
BspDI ATCGAT 1 cut(s) 1503
BspFNI CGCG 4 cut(s) 99, 231, 461, 1150
BspHI TCATGA 1 cut(s) 1212
BspLI GGNNCC 2 cut(s) 1227, 1399
BspQI GCTCTTC 1 cut(s) 1306
BspT107I GGYRCC 1 cut(s) 1397
BsrDI GCAATG 2 cut(s) 807, 896
BsrFI RCCGGY 1 cut(s) 833
BsrI ACTGG 1 cut(s) 1714
BssAI RCCGGY 1 cut(s) 833
BssECI CCNNGG 4 cut(s) 630, 670, 958, 1738
BssMI GATC 5 cut(s) 202, 256, 298, 322, 1725
BssNI GRCGYC 1 cut(s) 1398
BssSI CACGAG 1 cut(s) 1590
BssT1I CCWWGG 2 cut(s) 630, 958
Bst2BI CACGAG 1 cut(s) 1590
Bst2UI CCWGG 1 cut(s) 671
Bst4CI ACNGT 9 cut(s) 14, 20, 682, 703, 943, 1133, 1480, 1739, 1775
Bst6I CTCTTC 2 cut(s) 26, 1306
BstACI GRCGYC 1 cut(s) 1398
BstBAI YACGTR 1 cut(s) 182
BstC8I GCNNGC 2 cut(s) 154, 763
BstDEI CTNAG 1 cut(s) 1055
BstDSI CCRYGG 2 cut(s) 630, 1738
BstEII GGTNACC 2 cut(s) 386, 580
BstF5I GGATG 4 cut(s) 449, 626, 1036, 1282
BstFNI CGCG 4 cut(s) 99, 231, 461, 1150
BstH2I RGCGCY 2 cut(s) 423, 1401
BstHHI GCGC 3 cut(s) 101, 422, 1400
BstKTI GATC 5 cut(s) 205, 259, 301, 325, 1728
BstMAI GTCTC 1 cut(s) 942
BstMBI GATC 5 cut(s) 202, 256, 298, 322, 1725
BstMWI GCNNNNNNNGC 4 cut(s) 96, 809, 1397, 1530
BstNI CCWGG 1 cut(s) 671
BstPI GGTNACC 2 cut(s) 386, 580
BstSCI CCNGG 2 cut(s) 669, 1572
BstSFI CTRYAG 1 cut(s) 1331
BstUI CGCG 4 cut(s) 99, 231, 461, 1150
BstV1I GCAGC 4 cut(s) 74, 212, 787, 790
BstV2I GAAGAC 2 cut(s) 344, 975
BstXI CCANNNNNNTGG 2 cut(s) 248, 637
Bsu15I ATCGAT 1 cut(s) 1503
BsuRI GGCC 5 cut(s) 535, 594, 819, 833, 1713
BsuTUI ATCGAT 1 cut(s) 1503
BtgI CCRYGG 2 cut(s) 630, 1738
BtsCI GGATG 4 cut(s) 449, 626, 1036, 1282
BtsI GCAGTG 1 cut(s) 1276
BtsIMutI CAGTG 4 cut(s) 505, 699, 1276, 1707
BtuMI TCGCGA 1 cut(s) 461
Cac8I GCNNGC 2 cut(s) 154, 763
CaiI CAGNNNCTG 1 cut(s) 18
CciI TCATGA 1 cut(s) 1212
CfoI GCGC 3 cut(s) 101, 422, 1400
Cfr10I RCCGGY 1 cut(s) 833
Cfr13I GGNCC 1 cut(s) 554
ClaI ATCGAT 1 cut(s) 1503
CseI GACGC 1 cut(s) 719
Csp6I GTAC 3 cut(s) 62, 882, 1152
CspCI CAANNNNNGTGG 2 cut(s) 558, 593
CviAII CATG 9 cut(s) 221, 242, 302, 521, 631, 847, 907, 1213, 1258
CviQI GTAC 3 cut(s) 62, 882, 1152
DdeI CTNAG 1 cut(s) 1055
DinI GGCGCC 1 cut(s) 1399
DpnI GATC 5 cut(s) 204, 258, 300, 324, 1727
DpnII GATC 5 cut(s) 202, 256, 298, 322, 1725
EaeI YGGCCR 3 cut(s) 533, 592, 1711
Eam1104I CTCTTC 2 cut(s) 26, 1306
EarI CTCTTC 2 cut(s) 26, 1306
Ecl136II GAGCTC 1 cut(s) 1052
Eco130I CCWWGG 2 cut(s) 630, 958
Eco24I GRGCYC 2 cut(s) 677, 1054
Eco47I GGWCC 1 cut(s) 554
Eco53kI GAGCTC 1 cut(s) 1052
Eco91I GGTNACC 2 cut(s) 386, 580
EcoICRI GAGCTC 1 cut(s) 1052
EcoO65I GGTNACC 2 cut(s) 386, 580
EcoRI GAATTC 3 cut(s) 1007, 1308, 1446
EcoRII CCWGG 1 cut(s) 669
EcoT14I CCWWGG 2 cut(s) 630, 958
EcoT38I GRGCYC 2 cut(s) 677, 1054
EgeI GGCGCC 1 cut(s) 1399
EheI GGCGCC 1 cut(s) 1399
ErhI CCWWGG 2 cut(s) 630, 958
FaeI CATG 9 cut(s) 224, 245, 305, 524, 634, 850, 910, 1216, 1261
FaqI GGGAC 3 cut(s) 689, 1107, 1589
FatI CATG 9 cut(s) 220, 241, 301, 520, 630, 846, 906, 1212, 1257
FauI CCCGC 1 cut(s) 715
FauNDI CATATG 1 cut(s) 1393
Fnu4HI GCNGC 7 cut(s) 7, 88, 226, 229, 801, 804, 1401
FokI GGATG 4 cut(s) 456, 613, 1043, 1289
FriOI GRGCYC 2 cut(s) 677, 1054
Fsp4HI GCNGC 7 cut(s) 7, 88, 226, 229, 801, 804, 1401
FspBI CTAG 3 cut(s) 912, 1305, 1443
GlaI GCGC 3 cut(s) 100, 421, 1399
GluI GCNGC 7 cut(s) 7, 88, 226, 229, 801, 804, 1401
HaeII RGCGCY 2 cut(s) 423, 1401
HaeIII GGCC 5 cut(s) 535, 594, 819, 833, 1713
HapII CCGG 3 cut(s) 834, 1538, 1573
HgaI GACGC 1 cut(s) 719
HhaI GCGC 3 cut(s) 101, 422, 1400
Hin1I GRCGYC 1 cut(s) 1398
Hin1II CATG 9 cut(s) 224, 245, 305, 524, 634, 850, 910, 1216, 1261
Hin6I GCGC 3 cut(s) 99, 420, 1398
HinP1I GCGC 3 cut(s) 99, 420, 1398
HincII GTYRAC 1 cut(s) 991
HindII GTYRAC 1 cut(s) 991
HinfI GANTC 7 cut(s) 340, 722, 841, 1095, 1106, 1411, 1429
HpaII CCGG 3 cut(s) 834, 1538, 1573
HphI GGTGA 8 cut(s) 101, 398, 887, 932, 947, 977, 1153, 1571
Hpy166II GTNNAC 2 cut(s) 991, 1707
Hpy188I TCNGA 3 cut(s) 202, 364, 1111
Hpy8I GTNNAC 2 cut(s) 991, 1707
HpyAV CCTTC 5 cut(s) 545, 823, 830, 1075, 1345
HpyCH4III ACNGT 9 cut(s) 14, 20, 682, 703, 943, 1133, 1480, 1739, 1775
HpyCH4IV ACGT 3 cut(s) 181, 1281, 1461
HpyCH4V TGCA 9 cut(s) 9, 173, 245, 356, 692, 780, 1321, 1362, 1625
HpyF10VI GCNNNNNNNGC 4 cut(s) 96, 809, 1397, 1530
HpyF3I CTNAG 1 cut(s) 1055
HpySE526I ACGT 3 cut(s) 181, 1281, 1461
Hsp92I GRCGYC 1 cut(s) 1398
Hsp92II CATG 9 cut(s) 224, 245, 305, 524, 634, 850, 910, 1216, 1261
HspAI GCGC 3 cut(s) 99, 420, 1398
KasI GGCGCC 1 cut(s) 1397
Kzo9I GATC 5 cut(s) 202, 256, 298, 322, 1725
LguI GCTCTTC 1 cut(s) 1306
LmnI GCTCC 2 cut(s) 1225, 1354
Lsp1109I GCAGC 4 cut(s) 74, 212, 787, 790
LweI GCATC 4 cut(s) 434, 636, 736, 1657
MaeI CTAG 3 cut(s) 912, 1305, 1443
MaeII ACGT 3 cut(s) 181, 1281, 1461
MaeIII GTNAC 8 cut(s) 14, 386, 480, 500, 580, 1453, 1547, 1607
MalI GATC 5 cut(s) 204, 258, 300, 324, 1727
MboI GATC 5 cut(s) 202, 256, 298, 322, 1725
MboII GAAGA 8 cut(s) 43, 135, 245, 349, 629, 664, 980, 1293
MhlI GDGCHC 3 cut(s) 677, 1054, 1183
MlsI TGGCCA 3 cut(s) 535, 594, 1713
MluI ACGCGT 1 cut(s) 1148
MluNI TGGCCA 3 cut(s) 535, 594, 1713
Mly113I GGCGCC 1 cut(s) 1398
MlyI GAGTC 4 cut(s) 334, 716, 835, 1089
MmeI TCCRAC 3 cut(s) 818, 925, 1610
Mox20I TGGCCA 3 cut(s) 535, 594, 1713
MscI TGGCCA 3 cut(s) 535, 594, 1713
MseI TTAA 8 cut(s) 186, 399, 767, 786, 1064, 1371, 1482, 1659
MslI CAYNNNNRTG 3 cut(s) 47, 276, 629
Msp20I TGGCCA 3 cut(s) 535, 594, 1713
MspA1I CMGCKG 2 cut(s) 87, 560
MspI CCGG 3 cut(s) 834, 1538, 1573
MspR9I CCNGG 2 cut(s) 671, 1574
Mva1269I GAATGC 2 cut(s) 271, 356
MvaI CCWGG 1 cut(s) 671
MvnI CGCG 4 cut(s) 99, 231, 461, 1150
MwoI GCNNNNNNNGC 4 cut(s) 96, 809, 1397, 1530
NarI GGCGCC 1 cut(s) 1398
NciI CCSGG 1 cut(s) 1574
NcoI CCATGG 1 cut(s) 630
NdeI CATATG 1 cut(s) 1393
NdeII GATC 5 cut(s) 202, 256, 298, 322, 1725
NlaIII CATG 9 cut(s) 224, 245, 305, 524, 634, 850, 910, 1216, 1261
NlaIV GGNNCC 2 cut(s) 1227, 1399
NmuCI GTSAC 4 cut(s) 386, 500, 1453, 1607
NruI TCGCGA 1 cut(s) 461
PagI TCATGA 1 cut(s) 1212
PciSI GCTCTTC 1 cut(s) 1306
PctI GAATGC 2 cut(s) 271, 356
PfeI GAWTC 3 cut(s) 1106, 1411, 1429
PfoI TCCNGGA 1 cut(s) 1572
PkrI GCNGC 7 cut(s) 8, 89, 227, 230, 802, 805, 1402
PleI GAGTC 4 cut(s) 334, 716, 835, 1089
PluTI GGCGCC 1 cut(s) 1401
PpsI GAGTC 4 cut(s) 334, 716, 835, 1089
Ppu21I YACGTR 1 cut(s) 182
PshBI ATTAAT 2 cut(s) 186, 1371
Psp124BI GAGCTC 1 cut(s) 1054
Psp6I CCWGG 1 cut(s) 669
PspEI GGTNACC 2 cut(s) 386, 580
PspGI CCWGG 1 cut(s) 669
PspN4I GGNNCC 2 cut(s) 1227, 1399
PspPI GGNCC 1 cut(s) 554
PstNI CAGNNNCTG 1 cut(s) 18
PvuII CAGCTG 2 cut(s) 87, 560
RruI TCGCGA 1 cut(s) 461
RsaI GTAC 3 cut(s) 63, 883, 1153
RsaNI GTAC 3 cut(s) 62, 882, 1152
RseI CAYNNNNRTG 3 cut(s) 47, 276, 629
SacI GAGCTC 1 cut(s) 1054
SapI GCTCTTC 1 cut(s) 1306
SaqAI TTAA 8 cut(s) 186, 399, 767, 786, 1064, 1371, 1482, 1659
SatI GCNGC 7 cut(s) 7, 88, 226, 229, 801, 804, 1401
Sau3AI GATC 5 cut(s) 202, 256, 298, 322, 1725
Sau96I GGNCC 1 cut(s) 554
ScaI AGTACT 1 cut(s) 63
SchI GAGTC 4 cut(s) 334, 716, 835, 1089
ScrFI CCNGG 2 cut(s) 671, 1574
SduI GDGCHC 3 cut(s) 677, 1054, 1183
SfaNI GCATC 4 cut(s) 434, 636, 736, 1657
SfcI CTRYAG 1 cut(s) 1331
SfoI GGCGCC 1 cut(s) 1399
SinI GGWCC 1 cut(s) 554
SmiMI CAYNNNNRTG 3 cut(s) 47, 276, 629
SmlI CTYRAG 1 cut(s) 1681
SmoI CTYRAG 1 cut(s) 1681
SpeI ACTAGT 1 cut(s) 911
SsiI CCGC 8 cut(s) 97, 229, 454, 478, 649, 708, 1401, 1510
SspDI GGCGCC 1 cut(s) 1397
SspI AATATT 2 cut(s) 160, 196
SspMI CTAG 3 cut(s) 912, 1305, 1443
SstI GAGCTC 1 cut(s) 1054
StyD4I CCNGG 2 cut(s) 669, 1572
StyI CCWWGG 2 cut(s) 630, 958
TaaI ACNGT 9 cut(s) 14, 20, 682, 703, 943, 1133, 1480, 1739, 1775
TaiI ACGT 3 cut(s) 184, 1284, 1464
TaqI TCGA 1 cut(s) 1503
TatI WGTACW 1 cut(s) 61
TauI GCSGC 2 cut(s) 231, 1403
TfiI GAWTC 3 cut(s) 1106, 1411, 1429
Tru1I TTAA 8 cut(s) 186, 399, 767, 786, 1064, 1371, 1482, 1659
Tru9I TTAA 8 cut(s) 186, 399, 767, 786, 1064, 1371, 1482, 1659
TscAI CASTG 4 cut(s) 505, 706, 1276, 1714
TseFI GTSAC 4 cut(s) 386, 500, 1453, 1607
TseI GCWGC 5 cut(s) 6, 87, 225, 800, 803
Tsp45I GTSAC 4 cut(s) 386, 500, 1453, 1607
TspDTI ATGAA 5 cut(s) 65, 156, 1201, 1229, 1274
TspGWI ACGGA 1 cut(s) 72
TspRI CASTG 4 cut(s) 505, 706, 1276, 1714
VpaK11BI GGWCC 1 cut(s) 554
VspI ATTAAT 2 cut(s) 186, 1371
XapI RAATTY 5 cut(s) 494, 1007, 1308, 1446, 1596
XbaI TCTAGA 2 cut(s) 1304, 1442
XspI CTAG 3 cut(s) 912, 1305, 1443
ZrmI AGTACT 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.