MD12G1030400.v1.1
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
N/A
Physical Location & Seq
Forward (+)
3305860 .. 3307370
1511 bp
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UTR
Exon/CDS
Intron
MD12G1030400.v1.1.491

Sequence Viewer

Length: 564 bp
ATGCCCATTACTGTTTTGTGTCTTCTTTTTTTAACATTGGGCACCATAGATGGTGCCCTCTATGGAATGAGACTGTTTAAGCTTTTTGGTTGTCAAACAGACACAAAATTCTCAATTGTACCCTTTGTTTTGTACCCAAAGCTCATTTTTGTTGTAGTGTATGCCGCTGCCGGAGAGGCGATCCATGGAGATCCGAAGCAGTGGTTCTTTTTCATCCCAAGACAAGAAAGTGAAGCTCGAGGAGGGAGACCGAGACAACTCACAACAACTGGGTATTGGAAAGCAACAGGATCTCCAACCACTGTTTACTCTTCCAATTCCAAACACTATCGTGCCATCAGCCTCAAAAGAACCATGGTTTTCTATACTGGCAGAGCTCCCCATGGAAAGAAAACGGAGTGGAAGATGAATGAAACAAAGCCATTGAAATTCATGCAAATGATAATAATCAACCATCAATGGCCGCCTCCTCACCAAACCCTAGTACTCCTACTCCCACGGTACATACGTATTAAGGCAAGAATTCAGCTTATGTCGAGTATACAAGAAATCGAAATGCCTTAG

Protein Analysis

188

Amino Acids

21.54

Weight (kDa)

10.05

Isoelectric Point (pI)

35.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 63 - 138 2.5e-16 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 41, 53
AccI GTMKAC 1 cut(s) 541
AciI CCGC 2 cut(s) 165, 464
AclWI GGATC 3 cut(s) 175, 185, 298
AcoI YGGCCR 1 cut(s) 461
AcsI RAATTY 3 cut(s) 107, 428, 522
AfaI GTAC 4 cut(s) 120, 134, 486, 503
AgsI TTSAA 1 cut(s) 427
AleI CACNNNNGTG 1 cut(s) 330
AluBI AGCT 5 cut(s) 82, 142, 236, 377, 529
AluI AGCT 5 cut(s) 82, 142, 236, 377, 529
Alw21I GWGCWC 1 cut(s) 379
Alw26I GTCTC 3 cut(s) 64, 241, 247
AlwI GGATC 3 cut(s) 175, 185, 298
Ama87I CYCGRG 1 cut(s) 237
AoxI GGCC 1 cut(s) 461
ApeKI GCWGC 1 cut(s) 167
ApoI RAATTY 3 cut(s) 107, 428, 522
AsuHPI GGTGA 1 cut(s) 464
AvaI CYCGRG 1 cut(s) 237
BaeGI GKGCMC 2 cut(s) 44, 58
BaeI ACNNNNGTAYC 2 cut(s) 493, 526
BanI GGYRCC 2 cut(s) 41, 53
BanII GRGCYC 1 cut(s) 379
BbsI GAAGAC 1 cut(s) 14
Bbv12I GWGCWC 1 cut(s) 379
BbvI GCAGC 1 cut(s) 154
BccI CCATC 3 cut(s) 44, 344, 462
BcoDI GTCTC 3 cut(s) 64, 241, 247
BfaI CTAG 1 cut(s) 482
BglI GCCNNNNNGGC 1 cut(s) 176
BisI GCNGC 3 cut(s) 165, 168, 464
BlsI GCNGC 3 cut(s) 166, 169, 465
BmcAI AGTACT 1 cut(s) 486
BmeT110I CYCGRG 1 cut(s) 237
BmiI GGNNCC 2 cut(s) 43, 55
BmrI ACTGGG 1 cut(s) 279
BmuI ACTGGG 1 cut(s) 279
BpiI GAAGAC 1 cut(s) 14
BsaAI YACGTR 1 cut(s) 509
BsaBI GATNNNNATC 1 cut(s) 446
BsaI GGTCTC 1 cut(s) 241
BsaJI CCNNGG 4 cut(s) 184, 354, 382, 497
BsaXI ACNNNNNCTCC 4 cut(s) 277, 307, 477, 507
Bse1I ACTGG 2 cut(s) 274, 373
Bse8I GATNNNNATC 1 cut(s) 446
BseDI CCNNGG 4 cut(s) 184, 354, 382, 497
BseGI GGATG 1 cut(s) 213
BseJI GATNNNNATC 1 cut(s) 446
BseNI ACTGG 2 cut(s) 274, 373
BseRI GAGGAG 2 cut(s) 255, 459
BseSI GKGCMC 2 cut(s) 44, 58
BseXI GCAGC 1 cut(s) 154
BshFI GGCC 1 cut(s) 463
BshNI GGYRCC 2 cut(s) 41, 53
BsiHKAI GWGCWC 1 cut(s) 379
BsiHKCI CYCGRG 1 cut(s) 237
BsiSI CCGG 1 cut(s) 171
BsmAI GTCTC 3 cut(s) 64, 241, 247
BsnI GGCC 1 cut(s) 463
Bso31I GGTCTC 1 cut(s) 241
BsoBI CYCGRG 1 cut(s) 237
Bsp1286I GDGCHC 3 cut(s) 44, 58, 379
Bsp143I GATC 3 cut(s) 180, 190, 290
Bsp19I CCATGG 3 cut(s) 184, 354, 382
BspACI CCGC 2 cut(s) 165, 464
BspANI GGCC 1 cut(s) 463
BspLI GGNNCC 2 cut(s) 43, 55
BspPI GGATC 3 cut(s) 175, 185, 298
BspT107I GGYRCC 2 cut(s) 41, 53
BspTNI GGTCTC 1 cut(s) 241
BsrI ACTGG 2 cut(s) 274, 373
BssECI CCNNGG 4 cut(s) 184, 354, 382, 497
BssMI GATC 3 cut(s) 180, 190, 290
BssNAI GTATAC 1 cut(s) 542
BssT1I CCWWGG 3 cut(s) 184, 354, 382
Bst1107I GTATAC 1 cut(s) 542
Bst4CI ACNGT 4 cut(s) 13, 75, 304, 501
Bst6I CTCTTC 1 cut(s) 316
BstBAI YACGTR 1 cut(s) 509
BstDEI CTNAG 1 cut(s) 561
BstDSI CCRYGG 4 cut(s) 184, 354, 382, 497
BstF5I GGATG 1 cut(s) 213
BstKTI GATC 3 cut(s) 183, 193, 293
BstMAI GTCTC 3 cut(s) 64, 241, 247
BstMBI GATC 3 cut(s) 180, 190, 290
BstMWI GCNNNNNNNGC 1 cut(s) 176
BstSLI GKGCMC 2 cut(s) 44, 58
BstSNI TACGTA 1 cut(s) 509
BstV1I GCAGC 1 cut(s) 154
BstV2I GAAGAC 1 cut(s) 14
BstX2I RGATCY 2 cut(s) 190, 290
BstYI RGATCY 2 cut(s) 190, 290
BstZ17I GTATAC 1 cut(s) 542
BsuRI GGCC 1 cut(s) 463
BtgI CCRYGG 4 cut(s) 184, 354, 382, 497
BtsCI GGATG 1 cut(s) 213
BtsI GCAGTG 1 cut(s) 206
BtsIMutI CAGTG 2 cut(s) 206, 300
Csp6I GTAC 4 cut(s) 119, 133, 485, 502
CviAII CATG 4 cut(s) 185, 355, 383, 433
CviJI RGCY 8 cut(s) 82, 142, 236, 342, 377, 421, 463, 529
CviKI_1 RGCY 8 cut(s) 82, 142, 236, 342, 377, 421, 463, 529
CviQI GTAC 4 cut(s) 119, 133, 485, 502
DdeI CTNAG 1 cut(s) 561
DpnI GATC 3 cut(s) 182, 192, 292
DpnII GATC 3 cut(s) 180, 190, 290
EaeI YGGCCR 1 cut(s) 461
Eam1104I CTCTTC 1 cut(s) 316
EarI CTCTTC 1 cut(s) 316
Ecl136II GAGCTC 1 cut(s) 377
Eco105I TACGTA 1 cut(s) 509
Eco130I CCWWGG 3 cut(s) 184, 354, 382
Eco24I GRGCYC 1 cut(s) 379
Eco31I GGTCTC 1 cut(s) 241
Eco53kI GAGCTC 1 cut(s) 377
Eco88I CYCGRG 1 cut(s) 237
EcoICRI GAGCTC 1 cut(s) 377
EcoRI GAATTC 1 cut(s) 522
EcoT14I CCWWGG 3 cut(s) 184, 354, 382
EcoT38I GRGCYC 1 cut(s) 379
ErhI CCWWGG 3 cut(s) 184, 354, 382
FaeI CATG 4 cut(s) 188, 358, 386, 436
FatI CATG 4 cut(s) 184, 354, 382, 432
FblI GTMKAC 1 cut(s) 541
Fnu4HI GCNGC 3 cut(s) 165, 168, 464
FokI GGATG 1 cut(s) 200
FriOI GRGCYC 1 cut(s) 379
Fsp4HI GCNGC 3 cut(s) 165, 168, 464
FspBI CTAG 1 cut(s) 482
GluI GCNGC 3 cut(s) 165, 168, 464
HaeIII GGCC 1 cut(s) 463
HapII CCGG 1 cut(s) 171
Hin1II CATG 4 cut(s) 188, 358, 386, 436
HindIII AAGCTT 1 cut(s) 80
HpaII CCGG 1 cut(s) 171
HphI GGTGA 1 cut(s) 464
Hpy166II GTNNAC 2 cut(s) 307, 542
Hpy188I TCNGA 1 cut(s) 195
Hpy8I GTNNAC 2 cut(s) 307, 542
HpyCH4III ACNGT 4 cut(s) 13, 75, 304, 501
HpyCH4IV ACGT 1 cut(s) 508
HpyCH4V TGCA 1 cut(s) 436
HpyF10VI GCNNNNNNNGC 1 cut(s) 176
HpyF3I CTNAG 1 cut(s) 561
HpySE526I ACGT 1 cut(s) 508
Hsp92II CATG 4 cut(s) 188, 358, 386, 436
Kzo9I GATC 3 cut(s) 180, 190, 290
LmnI GCTCC 1 cut(s) 382
LpnPI CCDG 4 cut(s) 184, 255, 273, 354
Lsp1109I GCAGC 1 cut(s) 154
MaeI CTAG 1 cut(s) 482
MaeII ACGT 1 cut(s) 508
MalI GATC 3 cut(s) 182, 192, 292
MboI GATC 3 cut(s) 180, 190, 290
MboII GAAGA 3 cut(s) 14, 303, 415
MfeI CAATTG 1 cut(s) 114
MflI RGATCY 2 cut(s) 190, 290
MhlI GDGCHC 3 cut(s) 44, 58, 379
MluCI AATT 5 cut(s) 107, 114, 316, 428, 522
MmeI TCCRAC 1 cut(s) 320
MnlI CCTC 7 cut(s) 68, 169, 233, 236, 353, 477, 480
MseI TTAA 3 cut(s) 32, 78, 513
MslI CAYNNNNRTG 2 cut(s) 330, 437
MspA1I CMGCKG 1 cut(s) 167
MspI CCGG 1 cut(s) 171
MunI CAATTG 1 cut(s) 114
MwoI GCNNNNNNNGC 1 cut(s) 176
NcoI CCATGG 3 cut(s) 184, 354, 382
NdeII GATC 3 cut(s) 180, 190, 290
NlaIII CATG 4 cut(s) 188, 358, 386, 436
NlaIV GGNNCC 2 cut(s) 43, 55
OliI CACNNNNGTG 1 cut(s) 330
PaeR7I CTCGAG 1 cut(s) 237
PcsI WCGNNNNNNNCGW 1 cut(s) 505
PkrI GCNGC 3 cut(s) 166, 169, 465
Ppu21I YACGTR 1 cut(s) 509
Psp124BI GAGCTC 1 cut(s) 379
PspN4I GGNNCC 2 cut(s) 43, 55
PspXI VCTCGAGB 1 cut(s) 237
PsuI RGATCY 2 cut(s) 190, 290
RsaI GTAC 4 cut(s) 120, 134, 486, 503
RsaNI GTAC 4 cut(s) 119, 133, 485, 502
RseI CAYNNNNRTG 2 cut(s) 330, 437
SacI GAGCTC 1 cut(s) 379
SaqAI TTAA 3 cut(s) 32, 78, 513
SatI GCNGC 3 cut(s) 165, 168, 464
Sau3AI GATC 3 cut(s) 180, 190, 290
ScaI AGTACT 1 cut(s) 486
SduI GDGCHC 3 cut(s) 44, 58, 379
SetI ASST 6 cut(s) 84, 144, 238, 379, 511, 531
Sfr274I CTCGAG 1 cut(s) 237
SlaI CTCGAG 1 cut(s) 237
SmiMI CAYNNNNRTG 2 cut(s) 330, 437
SmlI CTYRAG 1 cut(s) 237
SmoI CTYRAG 1 cut(s) 237
SnaBI TACGTA 1 cut(s) 509
Sse9I AATT 5 cut(s) 107, 114, 316, 428, 522
SsiI CCGC 2 cut(s) 165, 464
SspMI CTAG 1 cut(s) 482
SstI GAGCTC 1 cut(s) 379
StyI CCWWGG 3 cut(s) 184, 354, 382
TaaI ACNGT 4 cut(s) 13, 75, 304, 501
TaiI ACGT 1 cut(s) 511
TaqI TCGA 3 cut(s) 238, 536, 552
TaqII GACCGA 1 cut(s) 265
TasI AATT 5 cut(s) 107, 114, 316, 428, 522
TatI WGTACW 1 cut(s) 484
TauI GCSGC 2 cut(s) 167, 466
Tru1I TTAA 3 cut(s) 32, 78, 513
Tru9I TTAA 3 cut(s) 32, 78, 513
TscAI CASTG 2 cut(s) 206, 307
TseI GCWGC 1 cut(s) 167
TspDTI ATGAA 4 cut(s) 202, 421, 422, 426
TspGWI ACGGA 1 cut(s) 410
TspRI CASTG 2 cut(s) 206, 307
XapI RAATTY 3 cut(s) 107, 428, 522
XhoI CTCGAG 1 cut(s) 237
XmiI GTMKAC 1 cut(s) 541
XspI CTAG 1 cut(s) 482
ZrmI AGTACT 1 cut(s) 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.