MD13G1230800.v1.1
TCP Family

Belongs to the chaperonin (HSP60) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
N/A
Physical Location & Seq
Reverse (-)
22675068 .. 22678921
3854 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1230800.v1.1.491

Sequence Viewer

Length: 1263 bp
ATGGAGAGGCTGCTCCCAAGTCTCGAACCCGAGACCTACCTCACATGGGCGAAGGCACTTGCCTATACAATTATATCTACTGGTGGGCGAAATGTGGTTATTGAACAAAGCTTTGGGGCACCCAAAGTGACAAAAGATGGTGTAACAGTTGCAAAAAGTATAGAGTTTAAGGATAAAGTCAAGAATGTTGGTGCTAGCCTAGTGAAACAGGTTGCTAATGCTATCAATGATGTAGCGGGTGATGGTACAACTTGTGCTACAATCTTGACCAAGGCTATTTCTACTGAAGGATGCAAGTCAGTTGCTGCCAGGATGAACGCAATGGACCTGCGACGTGGTATCACTTCGGCAGTTGATTCTGTGGTAACGAACTTGAAGAGCAGAGCACGAATGATCAGCACTTCTGAAGAGATAGCACAGGTTGGAACAATATCAGTGAATGGAGAGAGAGAAATTGGTGAGTTGATTGCAAAGGCCGTGGAGAAGGTTGGAAAAGAGGGAGTAATCACAATCTCTGATGGGAAAACATTGTACAATGACACTGTAATTCTTGATGTTGCTGGTGACAAGAAGGCCATTGATGAAAGATCTGAACAGATTAGGTCGGCAATTGAATTAAGCACTTCCGACTATGACAAGGAAAAGTTACAGGAGAGACTCGCAAAGCTTTCTGGTGGTGTTGCCGTTTTGAAGTTGTTGTTAACAATCATAATAACGATAAAAGTGAATCAACAAATGTCTGTGGCTGAGTTTCCTTGTATTAGTTGTATGTTGAGATTCGCTTATATTGCAATTGGAGGAGCTAGTGAAGCAGAAGTTAGTGATAAGAAGGATAGGGTTACCGATGCCTTAAATGCCACTAAAGCAGCCGTTGAAGAGGGTACAGTACCAGGTAATTTATTTTGTCATTTTGGCCGCTTTTGTTTGATGTTGCCTATTTGTGTTGGAGTTCAGATTATTCAGAATGCTCGAAAGGCACCTGTTCATACAATTGCTTCTAATGCGGGAGTTGAGGGAGCTGTTGTTGTTGGTAAGCTATTGGAGCAAGATAACACTGATCTTGGCTATGATGCAGCAAAAGGTGAATATGTTGACATGATTAAAGCTGGAGTAATCGATCCATTGAAAGTAATTAGAACTGCTTTAGTGGATGCTGCAAGTGTGTCGTCTTTGCTCACAACTACTGAAGCTGTTGTAGTTGAGCTTCCCAGTGAAAAGGAGACCCCGGCAATGGGTGGCGGCATGGGTGGAATGGATTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003735 GO:0005198 GO:0005488 GO:0005507 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0005773 GO:0005774 GO:0005829 GO:0005840 GO:0005844 GO:0005886 GO:0006457 GO:0006458 GO:0006605 GO:0006626 GO:0006810 GO:0006839 GO:0006886 GO:0006950 GO:0006996 GO:0007005 GO:0008104 GO:0008144 GO:0008150 GO:0009266 GO:0009408 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009719 GO:0009725 GO:0009735 GO:0009941 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0017038 GO:0017076 GO:0022626 GO:0030554 GO:0031090 GO:0031967 GO:0031974 GO:0031975 GO:0032553 GO:0032555 GO:0032559 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0035639 GO:0036094 GO:0042221 GO:0042788 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044183 GO:0044422 GO:0044424 GO:0044429 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0044743 GO:0045041 GO:0045184 GO:0046686 GO:0046872 GO:0046907 GO:0046914 GO:0050896 GO:0051082 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055085 GO:0061077 GO:0065002 GO:0070013 GO:0070585 GO:0070727 GO:0071702 GO:0071705 GO:0071806 GO:0071840 GO:0071944 GO:0072594 GO:0072655 GO:0097159 GO:0097367 GO:0098588 GO:0098805 GO:1901265 GO:1901363 GO:1990542 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03018 ko04212 ko04940 ko05134 ko05152 map03018 map04212 map04940 map05134 map05152
Pfam Domains
Protein Families
Cpn60_TCP1

Protein Analysis

421

Amino Acids

44.49

Weight (kDa)

5.36

Isoelectric Point (pI)

28.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cpn60_TCP1 PF00118 29 - 166 8.5e-20 TCP-1/cpn60 chaperonin family
Cpn60_TCP1 PF00118 316 - 400 3.2e-12 TCP-1/cpn60 chaperonin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 336
AccB1I GGYRCC 2 cut(s) 118, 976
AciI CCGC 4 cut(s) 236, 916, 1004, 1239
AclWI GGATC 1 cut(s) 1112
AcoI YGGCCR 1 cut(s) 913
AcuI CTGAAG 3 cut(s) 306, 426, 1206
AfaI GTAC 4 cut(s) 247, 533, 883, 888
AfiI CCNNNNNNNGG 3 cut(s) 46, 1231, 1232
AgsI TTSAA 6 cut(s) 104, 376, 614, 691, 875, 1126
AjiI CACGTC 1 cut(s) 335
AjnI CCWGG 2 cut(s) 308, 889
AjuI GAANNNNNNNTTGG 2 cut(s) 96, 128
AluBI AGCT 8 cut(s) 111, 667, 803, 1019, 1036, 1106, 1190, 1204
AluI AGCT 8 cut(s) 111, 667, 803, 1019, 1036, 1106, 1190, 1204
Alw21I GWGCWC 1 cut(s) 388
Alw26I GTCTC 4 cut(s) 26, 26, 649, 1214
AlwI GGATC 1 cut(s) 1112
AlwNI CAGNNNCTG 1 cut(s) 305
Ama87I CYCGRG 1 cut(s) 29
AoxI GGCC 3 cut(s) 474, 573, 913
ApeKI GCWGC 5 cut(s) 10, 305, 866, 1073, 1154
AspS9I GGNCC 1 cut(s) 325
AsuC2I CCSGG 1 cut(s) 1226
AsuHPI GGTGA 4 cut(s) 251, 470, 575, 1094
AsuNHI GCTAGC 1 cut(s) 194
AvaI CYCGRG 1 cut(s) 29
AvaII GGWCC 1 cut(s) 325
BaeGI GKGCMC 1 cut(s) 121
BanI GGYRCC 2 cut(s) 118, 976
BarI GAAGNNNNNNTAC 2 cut(s) 1188, 1220
Bbv12I GWGCWC 1 cut(s) 388
BbvI GCAGC 4 cut(s) 292, 878, 1085, 1141
BccI CCATC 3 cut(s) 131, 236, 512
BceAI ACGGC 3 cut(s) 461, 668, 854
BcgI CGANNNNNNTGC 2 cut(s) 1146, 1180
BciT130I CCWGG 2 cut(s) 310, 891
BclI TGATCA 1 cut(s) 393
BcnI CCSGG 1 cut(s) 1226
BcoDI GTCTC 4 cut(s) 26, 26, 649, 1214
BfaI CTAG 3 cut(s) 195, 200, 804
BfuAI ACCTGC 1 cut(s) 336
BglII AGATCT 1 cut(s) 587
BisI GCNGC 7 cut(s) 11, 306, 867, 916, 1074, 1155, 1240
BlsI GCNGC 7 cut(s) 12, 307, 868, 917, 1075, 1156, 1241
Bme1390I CCNGG 3 cut(s) 310, 891, 1226
Bme18I GGWCC 1 cut(s) 325
BmeT110I CYCGRG 1 cut(s) 29
BmgBI CACGTC 1 cut(s) 335
BmgT120I GGNCC 1 cut(s) 325
BmiI GGNNCC 2 cut(s) 120, 978
BmrFI CCNGG 3 cut(s) 310, 891, 1226
BmrI ACTGGG 1 cut(s) 1203
BmsI GCATC 4 cut(s) 281, 835, 1060, 1141
BmtI GCTAGC 1 cut(s) 198
BmuI ACTGGG 1 cut(s) 1203
BpmI CTGGAG 1 cut(s) 1128
BpuMI CCSGG 1 cut(s) 1226
Bsa29I ATCGAT 1 cut(s) 1116
BsaI GGTCTC 2 cut(s) 26, 1214
BsaJI CCNNGG 3 cut(s) 270, 477, 1224
BsaXI ACNNNNNCTCC 2 cut(s) 1008, 1038
Bsc4I CCNNNNNNNGG 3 cut(s) 46, 1231, 1232
Bse1I ACTGG 2 cut(s) 85, 1209
Bse3DI GCAATG 2 cut(s) 327, 1236
BseBI CCWGG 2 cut(s) 310, 891
BseCI ATCGAT 1 cut(s) 1116
BseDI CCNNGG 3 cut(s) 270, 477, 1224
BseGI GGATG 3 cut(s) 296, 318, 1156
BseLI CCNNNNNNNGG 3 cut(s) 46, 1231, 1232
BseMI GCAATG 2 cut(s) 327, 1236
BseMII CTCAG 1 cut(s) 738
BseNI ACTGG 2 cut(s) 85, 1209
BseRI GAGGAG 1 cut(s) 813
BseSI GKGCMC 1 cut(s) 121
BseXI GCAGC 4 cut(s) 292, 878, 1085, 1141
BshFI GGCC 3 cut(s) 476, 575, 915
BshNI GGYRCC 2 cut(s) 118, 976
BshVI ATCGAT 1 cut(s) 1116
BsiHKAI GWGCWC 1 cut(s) 388
BsiHKCI CYCGRG 1 cut(s) 29
BsiSI CCGG 1 cut(s) 1226
BslI CCNNNNNNNGG 3 cut(s) 46, 1231, 1232
BsmAI GTCTC 4 cut(s) 26, 26, 649, 1214
BsmI GAATGC 1 cut(s) 970
BsnI GGCC 3 cut(s) 476, 575, 915
Bso31I GGTCTC 2 cut(s) 26, 1214
BsoBI CYCGRG 1 cut(s) 29
Bsp1286I GDGCHC 2 cut(s) 121, 388
Bsp1407I TGTACA 1 cut(s) 531
Bsp143I GATC 4 cut(s) 393, 587, 1057, 1117
BspACI CCGC 4 cut(s) 236, 916, 1004, 1239
BspANI GGCC 3 cut(s) 476, 575, 915
BspCNI CTCAG 1 cut(s) 739
BspDI ATCGAT 1 cut(s) 1116
BspLI GGNNCC 2 cut(s) 120, 978
BspMI ACCTGC 1 cut(s) 336
BspOI GCTAGC 1 cut(s) 198
BspPI GGATC 1 cut(s) 1112
BspQI GCTCTTC 1 cut(s) 371
BspT107I GGYRCC 2 cut(s) 118, 976
BspTNI GGTCTC 2 cut(s) 26, 1214
BsrDI GCAATG 2 cut(s) 327, 1236
BsrGI TGTACA 1 cut(s) 531
BsrI ACTGG 2 cut(s) 85, 1209
BssECI CCNNGG 3 cut(s) 270, 477, 1224
BssMI GATC 4 cut(s) 393, 587, 1057, 1117
BssT1I CCWWGG 1 cut(s) 270
Bst2UI CCWGG 2 cut(s) 310, 891
Bst4CI ACNGT 3 cut(s) 148, 544, 886
Bst6I CTCTTC 3 cut(s) 371, 402, 870
BstAUI TGTACA 1 cut(s) 531
BstC8I GCNNGC 1 cut(s) 196
BstDEI CTNAG 1 cut(s) 747
BstDSI CCRYGG 1 cut(s) 477
BstEII GGTNACC 1 cut(s) 838
BstF5I GGATG 3 cut(s) 296, 318, 1156
BstKTI GATC 4 cut(s) 396, 590, 1060, 1120
BstMAI GTCTC 4 cut(s) 26, 26, 649, 1214
BstMBI GATC 4 cut(s) 393, 587, 1057, 1117
BstMWI GCNNNNNNNGC 7 cut(s) 788, 809, 854, 863, 974, 1001, 1042
BstNI CCWGG 2 cut(s) 310, 891
BstPI GGTNACC 1 cut(s) 838
BstSCI CCNGG 3 cut(s) 308, 889, 1224
BstSLI GKGCMC 1 cut(s) 121
BstV1I GCAGC 4 cut(s) 292, 878, 1085, 1141
BstX2I RGATCY 1 cut(s) 587
BstYI RGATCY 1 cut(s) 587
Bsu15I ATCGAT 1 cut(s) 1116
BsuRI GGCC 3 cut(s) 476, 575, 915
BsuTUI ATCGAT 1 cut(s) 1116
BtgI CCRYGG 1 cut(s) 477
BtrI CACGTC 1 cut(s) 335
BtsCI GGATG 3 cut(s) 296, 318, 1156
BtsIMutI CAGTG 4 cut(s) 441, 540, 1053, 1216
BveI ACCTGC 1 cut(s) 336
Cac8I GCNNGC 1 cut(s) 196
CaiI CAGNNNCTG 1 cut(s) 305
Cfr13I GGNCC 1 cut(s) 325
ClaI ATCGAT 1 cut(s) 1116
CsiI ACCWGGT 1 cut(s) 889
Csp6I GTAC 4 cut(s) 246, 532, 882, 887
CspCI CAANNNNNGTGG 2 cut(s) 459, 494
CviAII CATG 3 cut(s) 45, 1096, 1243
CviQI GTAC 4 cut(s) 246, 532, 882, 887
DdeI CTNAG 1 cut(s) 747
DpnI GATC 4 cut(s) 395, 589, 1059, 1119
DpnII GATC 4 cut(s) 393, 587, 1057, 1117
EaeI YGGCCR 1 cut(s) 913
Eam1104I CTCTTC 3 cut(s) 371, 402, 870
EarI CTCTTC 3 cut(s) 371, 402, 870
Eco130I CCWWGG 1 cut(s) 270
Eco31I GGTCTC 2 cut(s) 26, 1214
Eco47I GGWCC 1 cut(s) 325
Eco57I CTGAAG 3 cut(s) 306, 426, 1206
Eco88I CYCGRG 1 cut(s) 29
Eco91I GGTNACC 1 cut(s) 838
EcoO65I GGTNACC 1 cut(s) 838
EcoRII CCWGG 2 cut(s) 308, 889
EcoT14I CCWWGG 1 cut(s) 270
ErhI CCWWGG 1 cut(s) 270
FaeI CATG 3 cut(s) 48, 1099, 1246
FatI CATG 3 cut(s) 44, 1095, 1242
FauI CCCGC 2 cut(s) 229, 997
FbaI TGATCA 1 cut(s) 393
Fnu4HI GCNGC 7 cut(s) 11, 306, 867, 916, 1074, 1155, 1240
FokI GGATG 3 cut(s) 303, 325, 1163
Fsp4HI GCNGC 7 cut(s) 11, 306, 867, 916, 1074, 1155, 1240
FspBI CTAG 3 cut(s) 195, 200, 804
GluI GCNGC 7 cut(s) 11, 306, 867, 916, 1074, 1155, 1240
GsuI CTGGAG 1 cut(s) 1128
HaeIII GGCC 3 cut(s) 476, 575, 915
HapII CCGG 1 cut(s) 1226
Hin1II CATG 3 cut(s) 48, 1099, 1246
HincII GTYRAC 2 cut(s) 702, 1093
HindII GTYRAC 2 cut(s) 702, 1093
HindIII AAGCTT 2 cut(s) 109, 665
HinfI GANTC 4 cut(s) 356, 657, 727, 777
HpaI GTTAAC 1 cut(s) 702
HpaII CCGG 1 cut(s) 1226
HphI GGTGA 4 cut(s) 251, 470, 575, 1094
Hpy166II GTNNAC 2 cut(s) 702, 1093
Hpy188I TCNGA 6 cut(s) 406, 517, 592, 628, 954, 963
Hpy188III TCNNGA 4 cut(s) 23, 181, 265, 551
Hpy8I GTNNAC 2 cut(s) 702, 1093
Hpy99I CGWCG 1 cut(s) 336
HpyAV CCTTC 5 cut(s) 46, 281, 478, 565, 823
HpyCH4III ACNGT 3 cut(s) 148, 544, 886
HpyCH4IV ACGT 1 cut(s) 334
HpyCH4V TGCA 6 cut(s) 152, 294, 470, 791, 1073, 1157
HpyF10VI GCNNNNNNNGC 7 cut(s) 788, 809, 854, 863, 974, 1001, 1042
HpyF3I CTNAG 1 cut(s) 747
HpySE526I ACGT 1 cut(s) 334
Hsp92II CATG 3 cut(s) 48, 1099, 1246
Ksp22I TGATCA 1 cut(s) 393
KspAI GTTAAC 1 cut(s) 702
Kzo9I GATC 4 cut(s) 393, 587, 1057, 1117
LguI GCTCTTC 1 cut(s) 371
LmnI GCTCC 4 cut(s) 18, 800, 1016, 1042
Lsp1109I GCAGC 4 cut(s) 292, 878, 1085, 1141
LweI GCATC 4 cut(s) 281, 835, 1060, 1141
MabI ACCWGGT 1 cut(s) 889
MaeI CTAG 3 cut(s) 195, 200, 804
MaeII ACGT 1 cut(s) 334
MaeIII GTNAC 6 cut(s) 127, 142, 364, 563, 645, 838
MalI GATC 4 cut(s) 395, 589, 1059, 1119
MboI GATC 4 cut(s) 393, 587, 1057, 1117
MboII GAAGA 3 cut(s) 388, 419, 887
MfeI CAATTG 3 cut(s) 609, 792, 990
MflI RGATCY 1 cut(s) 587
MhlI GDGCHC 2 cut(s) 121, 388
MluCI AATT 9 cut(s) 69, 453, 546, 609, 614, 792, 895, 990, 1131
MlyI GAGTC 1 cut(s) 651
MmeI TCCRAC 4 cut(s) 403, 469, 651, 925
MnlI CCTC 5 cut(s) 50, 490, 791, 871, 1006
MseI TTAA 5 cut(s) 168, 617, 701, 851, 1101
MspI CCGG 1 cut(s) 1226
MspR9I CCNGG 3 cut(s) 310, 891, 1226
MunI CAATTG 3 cut(s) 609, 792, 990
Mva1269I GAATGC 1 cut(s) 970
MvaI CCWGG 2 cut(s) 310, 891
MwoI GCNNNNNNNGC 7 cut(s) 788, 809, 854, 863, 974, 1001, 1042
NciI CCSGG 1 cut(s) 1226
NdeII GATC 4 cut(s) 393, 587, 1057, 1117
NheI GCTAGC 1 cut(s) 194
NlaIII CATG 3 cut(s) 48, 1099, 1246
NlaIV GGNNCC 2 cut(s) 120, 978
NmuCI GTSAC 2 cut(s) 127, 563
PciSI GCTCTTC 1 cut(s) 371
PctI GAATGC 1 cut(s) 970
PfeI GAWTC 3 cut(s) 356, 727, 777
PkrI GCNGC 7 cut(s) 12, 307, 868, 917, 1075, 1156, 1241
PleI GAGTC 1 cut(s) 651
PpsI GAGTC 1 cut(s) 651
Psp6I CCWGG 2 cut(s) 308, 889
PspEI GGTNACC 1 cut(s) 838
PspGI CCWGG 2 cut(s) 308, 889
PspN4I GGNNCC 2 cut(s) 120, 978
PspPI GGNCC 1 cut(s) 325
PstNI CAGNNNCTG 1 cut(s) 305
PsuI RGATCY 1 cut(s) 587
RsaI GTAC 4 cut(s) 247, 533, 883, 888
RsaNI GTAC 4 cut(s) 246, 532, 882, 887
SapI GCTCTTC 1 cut(s) 371
SaqAI TTAA 5 cut(s) 168, 617, 701, 851, 1101
SatI GCNGC 7 cut(s) 11, 306, 867, 916, 1074, 1155, 1240
Sau3AI GATC 4 cut(s) 393, 587, 1057, 1117
Sau96I GGNCC 1 cut(s) 325
SchI GAGTC 1 cut(s) 651
ScrFI CCNGG 3 cut(s) 310, 891, 1226
SduI GDGCHC 2 cut(s) 121, 388
SexAI ACCWGGT 1 cut(s) 889
SfaNI GCATC 4 cut(s) 281, 835, 1060, 1141
SinI GGWCC 1 cut(s) 325
Sse9I AATT 9 cut(s) 69, 453, 546, 609, 614, 792, 895, 990, 1131
SsiI CCGC 4 cut(s) 236, 916, 1004, 1239
SspMI CTAG 3 cut(s) 195, 200, 804
StyD4I CCNGG 3 cut(s) 308, 889, 1224
StyI CCWWGG 1 cut(s) 270
TaaI ACNGT 3 cut(s) 148, 544, 886
TaiI ACGT 1 cut(s) 337
TaqI TCGA 3 cut(s) 24, 970, 1116
TasI AATT 9 cut(s) 69, 453, 546, 609, 614, 792, 895, 990, 1131
TatI WGTACW 1 cut(s) 531
TauI GCSGC 2 cut(s) 918, 1242
TfiI GAWTC 3 cut(s) 356, 727, 777
Tru1I TTAA 5 cut(s) 168, 617, 701, 851, 1101
Tru9I TTAA 5 cut(s) 168, 617, 701, 851, 1101
TscAI CASTG 4 cut(s) 441, 547, 1060, 1216
TseFI GTSAC 2 cut(s) 127, 563
TseI GCWGC 5 cut(s) 10, 305, 866, 1073, 1154
Tsp45I GTSAC 2 cut(s) 127, 563
TspDTI ATGAA 3 cut(s) 329, 597, 974
TspRI CASTG 4 cut(s) 441, 547, 1060, 1216
VpaK11BI GGWCC 1 cut(s) 325
XspI CTAG 3 cut(s) 195, 200, 804
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.