Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

N/A

Physical Location & Seq

Genomic Coordinates Forward (+)

944466 .. 947133

Gene Structure 2668 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1009400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1794 bp

ATGCACCACCAATTGTGTCCTCATACAAAGAGAAAATCCGTCCTTTTGCTCGACGCAATTGACAAACTTTGTAACTTAAGGATAATGGAAGAAGGTATTCCGCTCTCCACAATTGTAGTGGTTGGTGATCAATCTTCCGGTAAATCAAGTGTGCTGGAATCGTTGGCCGGCATTAGCCTCCTTAGGGGTCAAGGTATCTGTACAAGGGTACCTCTTATAATGAGGTTTCAACACAGTCCTCAGCCTGAGCTTTCTTTGGAGTGTAACAGCAAAGTTGATCGTACCGACGAAAACAATATTTCTGATGATATTGTCAAAGCCACTAATTCTATTGCAGGAGGAGGGATATCTAATACCCCATTGACATTGCTGGTTAAAAAGAACGGCGTTCCTGATTTGACGATGGTCGATCTTCCTGGGATCACTAGAGTCCCAGTTCAGGGTCAGCCTGAAAATATTTATGATCAAATCAAGGATATGATCATGCAATATATCAAGCCTGAGGAGAGTATAATTCTTAGACCTAGTTTGGGAGCTTATTTTGGAAGCTGCTGTGAGAATAAGCTTAAATCAAAGAAAAAAGCTAAAGCTGCTATTTGTAGCTTTGGAAAACTGGCTTTTTTTCAAAGCACACGGAGCTACAATGCTTCTTTAATGAAAAGACCCAATACTAGACTGCTTTTTTTTCCAAAAGCACTTTTACAAAAAAGTTTACCAAACACTCTGCTGATTTATTTCACAGCCGCTTATTCTCACAGCATAGCCGCTTATTCTCACAGCAGCTTTTTTTCAAAGCACAGCAATACCAAACCAGCCCTTAATGTCCTGTCTGCTAGTGTTGATTTTGCCACTTGCGAATCGATTAGGATGTCACAGAGCGTGGACAAGACCGGTGAAAGGACTCTGGCGCTAGTGATGAAGGTCGATAAGGCACCCGAAGGATTGCTTGAGAAGGTGACAGGAGACGATGTCAATATTGGGCTAGGTTATGTCTGCGTGAGGAACCGCATTGGTGATGAAACATACGAGGAGGCAGGGGCTATTACTCAGGATTTATTTCAATCTCATCCTCTTCTATCTAATATCGAAAAATCTATGGTTATTCCAGTTTTAGCACAAAGGTTGGTGCAGATTCAGGCTATTGGCATAGCGAGAAATTTACCAGAGATAGTAAAGAAAATCAACGAGAAGTTGAATTCCAGCCTCGCAGAGCTCAACAAGATGCCTAAGAGTCTGACATCCGTTGCTGAGGCTATGACAGCTTTCATGCAGATCATTGGATCGGCGAAAAAATCACTCAGGAAATTTCTTCTGAGAGGCGAATTTGAAGAGTATTCTGATGATCGCCACATGCATTGCACTGTGCGAATGAGCAAAGTGAACGGAATTTCAAGCATTCCTCTTGGGTTTGTGGAGAAGATCTGGAGTTATATAGAGGATGTTGTTGTTTCAGTGTTAATGCGACATTTAGAAAATTATTATAAGCTTCAGCTGTCCGCCAAACGAGTAGGCCAGAATCTTATGTCGAAGATGAAAGAAAGGTCGATTACTTGGATGACGGAGATAGTTGAAATTGAGAAAGTTACTGATTATACATGTAATCCGGAGTACCTCTCTGAATGGAATAGATTGATAGGTCAGCATGACGCTTTCGTTAACGGAGTCTTAAAAGACAAAAACCGCCCAGCTAAGATAACTATAGAGGGCATAGGGGAGGTTGAAGTTGAAGCTCTTAGGAAGAATCTGAACACTCTTTCGGCGGCGTATAACCTGAAAATGCGGATGATTGTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000266 GO:0003674 GO:0003824 GO:0003924 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006996 GO:0007005 GO:0008017 GO:0008092 GO:0008150 GO:0009987 GO:0015631 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017111 GO:0044424 GO:0044464 GO:0048285 GO:0071840

KEGG Pathways

Metabolic & Signaling

ko04139 ko04214 ko04217 ko04621 ko04668 ko05162 ko05164 ko05165 map04139 map04214 map04217 map04621 map04668 map05162 map05164 map05165

Pfam Domains

Protein Families

Dynamin_M Dynamin_N GED

Protein Analysis

598

Amino Acids

66.57

Weight (kDa)

9.04

Isoelectric Point (pI)

40.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Dynamin_N	PF00350	38 - 173	8.3e-28	Dynamin family
Dynamin_M	PF01031	315 - 459	3.2e-27	Dynamin central region
Dynamin_M	PF01031	459 - 543	1.6e-12	Dynamin central region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	218, 1482
Acc65I	GGTACC	1 cut(s)	208
AccB1I	GGYRCC	2 cut(s)	208, 931
AccBSI	CCGCTC	1 cut(s)	103
AccIII	TCCGGA	1 cut(s)	1603
AciI	CCGC	8 cut(s)	101, 744, 765, 1006, 1497, 1681, 1760, 1780
AclWI	GGATC	2 cut(s)	428, 1288
AcoI	YGGCCR	1 cut(s)	165
AcsI	RAATTY	5 cut(s)	1156, 1195, 1304, 1322, 1386
AcuI	CTGAAG	1 cut(s)	1472
AfaI	GTAC	4 cut(s)	202, 210, 283, 1610
AfiI	CCNNNNNNNGG	5 cut(s)	184, 439, 440, 530, 897
AflII	CTTAAG	1 cut(s)	76
AflIII	ACRYGT	1 cut(s)	1595
AgeI	ACCGGT	1 cut(s)	890
AjnI	CCWGG	1 cut(s)	415
Alw21I	GWGCWC	1 cut(s)	1215
Alw26I	GTCTC	1 cut(s)	957
AlwI	GGATC	2 cut(s)	428, 1288
Aor13HI	TCCGGA	1 cut(s)	1603
AoxI	GGCC	2 cut(s)	165, 1510
ApeKI	GCWGC	3 cut(s)	549, 590, 780
ApoI	RAATTY	5 cut(s)	1156, 1195, 1304, 1322, 1386
AsiGI	ACCGGT	1 cut(s)	890
Asp700I	GAANNNNTTC	1 cut(s)	96
Asp718I	GGTACC	1 cut(s)	208
AspLEI	GCGC	1 cut(s)	910
AsuHPI	GGTGA	4 cut(s)	137, 905, 967, 1025
AxyI	CCTNAGG	2 cut(s)	182, 501
BaeI	ACNNNNGTAYC	2 cut(s)	192, 225
BanI	GGYRCC	2 cut(s)	208, 931
BanII	GRGCYC	1 cut(s)	1215
Bbv12I	GWGCWC	1 cut(s)	1215
BbvCI	CCTCAGC	2 cut(s)	240, 1248
BbvI	GCAGC	3 cut(s)	536, 577, 792
BccI	CCATC	1 cut(s)	397
BceAI	ACGGC	1 cut(s)	400
BciT130I	CCWGG	1 cut(s)	417
BclI	TGATCA	3 cut(s)	127, 463, 480
BcoDI	GTCTC	1 cut(s)	957
BfaI	CTAG	6 cut(s)	426, 525, 672, 834, 911, 983
BfmI	CTRYAG	1 cut(s)	1698
BfoI	RGCGCY	1 cut(s)	911
BfrI	CTTAAG	1 cut(s)	76
BglII	AGATCT	1 cut(s)	1419
BisI	GCNGC	6 cut(s)	550, 591, 744, 765, 781, 1761
BlsI	GCNGC	6 cut(s)	551, 592, 745, 766, 782, 1762
Bme1390I	CCNGG	1 cut(s)	417
BmiI	GGNNCC	3 cut(s)	210, 933, 1004
BmrFI	CCNGG	1 cut(s)	417
BmrI	ACTGGG	1 cut(s)	428
BmsI	GCATC	1 cut(s)	1212
BmuI	ACTGGG	1 cut(s)	428
BoxI	GACNNNNGTC	1 cut(s)	404
BplI	GAGNNNNNCTC	2 cut(s)	1598, 1630
BpmI	CTGGAG	1 cut(s)	1444
Bpu10I	CCTNAGC	3 cut(s)	240, 246, 1248
BpuEI	CTTGAG	1 cut(s)	968
Bsa29I	ATCGAT	1 cut(s)	860
BsaJI	CCNNGG	1 cut(s)	416
BsaWI	WCCGGW	3 cut(s)	137, 890, 1603
BsaXI	ACNNNNNCTCC	2 cut(s)	954, 984
Bsc4I	CCNNNNNNNGG	5 cut(s)	184, 439, 440, 530, 897
Bse118I	RCCGGY	2 cut(s)	167, 890
Bse1I	ACTGG	3 cut(s)	434, 618, 1106
Bse21I	CCTNAGG	2 cut(s)	182, 501
Bse3DI	GCAATG	2 cut(s)	365, 1354
BseAI	TCCGGA	1 cut(s)	1603
BseBI	CCWGG	1 cut(s)	417
BseCI	ATCGAT	1 cut(s)	860
BseDI	CCNNGG	1 cut(s)	416
BseGI	GGATG	6 cut(s)	873, 1066, 1238, 1444, 1560, 1788
BseLI	CCNNNNNNNGG	5 cut(s)	184, 439, 440, 530, 897
BseMI	GCAATG	2 cut(s)	365, 1354
BseMII	CTCAG	7 cut(s)	237, 254, 492, 1061, 1239, 1304, 1312
BseNI	ACTGG	3 cut(s)	434, 618, 1106
BseRI	GAGGAG	3 cut(s)	354, 518, 1043
BseXI	GCAGC	3 cut(s)	536, 577, 792
BseYI	CCCAGC	1 cut(s)	1684
BsgI	GTGCAG	1 cut(s)	1148
BshFI	GGCC	2 cut(s)	167, 1512
BshNI	GGYRCC	2 cut(s)	208, 931
BshTI	ACCGGT	1 cut(s)	890
BshVI	ATCGAT	1 cut(s)	860
BsiHKAI	GWGCWC	1 cut(s)	1215
BsiSI	CCGG	4 cut(s)	138, 168, 891, 1604
BslFI	GGGAC	1 cut(s)	416
BslI	CCNNNNNNNGG	5 cut(s)	184, 439, 440, 530, 897
BsmAI	GTCTC	1 cut(s)	957
BsmBI	CGTCTC	1 cut(s)	957
BsmFI	GGGAC	1 cut(s)	416
BsmI	GAATGC	1 cut(s)	1395
BsnI	GGCC	2 cut(s)	167, 1512
Bsp1286I	GDGCHC	1 cut(s)	1215
Bsp13I	TCCGGA	1 cut(s)	1603
Bsp1407I	TGTACA	1 cut(s)	200
BspACI	CCGC	8 cut(s)	101, 744, 765, 1006, 1497, 1681, 1760, 1780
BspANI	GGCC	2 cut(s)	167, 1512
BspCNI	CTCAG	7 cut(s)	238, 253, 493, 1060, 1240, 1305, 1311
BspDI	ATCGAT	1 cut(s)	860
BspEI	TCCGGA	1 cut(s)	1603
BspLI	GGNNCC	3 cut(s)	210, 933, 1004
BspPI	GGATC	2 cut(s)	428, 1288
BspT107I	GGYRCC	2 cut(s)	208, 931
BspTI	CTTAAG	1 cut(s)	76
BsrBI	CCGCTC	1 cut(s)	103
BsrDI	GCAATG	2 cut(s)	365, 1354
BsrFI	RCCGGY	2 cut(s)	167, 890
BsrGI	TGTACA	1 cut(s)	200
BsrI	ACTGG	3 cut(s)	434, 618, 1106
BssAI	RCCGGY	2 cut(s)	167, 890
BssECI	CCNNGG	1 cut(s)	416
Bst2UI	CCWGG	1 cut(s)	417
Bst4CI	ACNGT	2 cut(s)	236, 1363
Bst6I	CTCTTC	2 cut(s)	1077, 1323
BstAFI	CTTAAG	1 cut(s)	76
BstAUI	TGTACA	1 cut(s)	200
BstC8I	GCNNGC	1 cut(s)	169
BstF5I	GGATG	6 cut(s)	873, 1066, 1238, 1444, 1560, 1788
BstH2I	RGCGCY	1 cut(s)	911
BstHHI	GCGC	1 cut(s)	910
BstMAI	GTCTC	1 cut(s)	957
BstMWI	GCNNNNNNNGC	3 cut(s)	590, 636, 1259
BstNI	CCWGG	1 cut(s)	417
BstNSI	RCATGY	2 cut(s)	1354, 1599
BstPAI	GACNNNNGTC	1 cut(s)	404
BstSCI	CCNGG	1 cut(s)	415
BstSFI	CTRYAG	1 cut(s)	1698
BstV1I	GCAGC	3 cut(s)	536, 577, 792
BstX2I	RGATCY	1 cut(s)	1419
BstYI	RGATCY	1 cut(s)	1419
Bsu15I	ATCGAT	1 cut(s)	860
Bsu36I	CCTNAGG	2 cut(s)	182, 501
BsuRI	GGCC	2 cut(s)	167, 1512
BsuTUI	ATCGAT	1 cut(s)	860
BtsCI	GGATG	6 cut(s)	873, 1066, 1238, 1444, 1560, 1788
BtsIMutI	CAGTG	2 cut(s)	1359, 1458
Cac8I	GCNNGC	1 cut(s)	169
CfoI	GCGC	1 cut(s)	910
Cfr10I	RCCGGY	2 cut(s)	167, 890
ClaI	ATCGAT	1 cut(s)	860
CseI	GACGC	2 cut(s)	62, 1655
Csp6I	GTAC	4 cut(s)	201, 209, 282, 1609
CspAI	ACCGGT	1 cut(s)	890
CspCI	CAANNNNNGTGG	4 cut(s)	99, 134, 1337, 1372
CviAII	CATG	5 cut(s)	484, 1267, 1351, 1596, 1643
CviQI	GTAC	4 cut(s)	201, 209, 282, 1609
EaeI	YGGCCR	1 cut(s)	165
Eam1104I	CTCTTC	2 cut(s)	1077, 1323
EarI	CTCTTC	2 cut(s)	1077, 1323
EciI	GGCGGA	1 cut(s)	1486
Ecl136II	GAGCTC	1 cut(s)	1213
Eco24I	GRGCYC	1 cut(s)	1215
Eco32I	GATATC	1 cut(s)	348
Eco53kI	GAGCTC	1 cut(s)	1213
Eco57I	CTGAAG	1 cut(s)	1472
Eco81I	CCTNAGG	2 cut(s)	182, 501
EcoICRI	GAGCTC	1 cut(s)	1213
EcoRI	GAATTC	1 cut(s)	1195
EcoRII	CCWGG	1 cut(s)	415
EcoRV	GATATC	1 cut(s)	348
EcoT22I	ATGCAT	1 cut(s)	1356
EcoT38I	GRGCYC	1 cut(s)	1215
Esp3I	CGTCTC	1 cut(s)	957
FaeI	CATG	5 cut(s)	487, 1270, 1354, 1599, 1646
FalI	AAGNNNNNCTT	2 cut(s)	930, 962
FaqI	GGGAC	1 cut(s)	416
FatI	CATG	5 cut(s)	483, 1266, 1350, 1595, 1642
FbaI	TGATCA	3 cut(s)	127, 463, 480
Fnu4HI	GCNGC	6 cut(s)	550, 591, 744, 765, 781, 1761
FokI	GGATG	5 cut(s)	880, 1053, 1225, 1451, 1567
FriOI	GRGCYC	1 cut(s)	1215
Fsp4HI	GCNGC	6 cut(s)	550, 591, 744, 765, 781, 1761
FspBI	CTAG	6 cut(s)	426, 525, 672, 834, 911, 983
GlaI	GCGC	1 cut(s)	909
GluI	GCNGC	6 cut(s)	550, 591, 744, 765, 781, 1761
GsaI	CCCAGC	1 cut(s)	1688
GsuI	CTGGAG	1 cut(s)	1444
HaeII	RGCGCY	1 cut(s)	911
HaeIII	GGCC	2 cut(s)	167, 1512
HapII	CCGG	4 cut(s)	138, 168, 891, 1604
HgaI	GACGC	2 cut(s)	62, 1655
HhaI	GCGC	1 cut(s)	910
Hin1II	CATG	5 cut(s)	487, 1270, 1354, 1599, 1646
Hin6I	GCGC	1 cut(s)	908
HinP1I	GCGC	1 cut(s)	908
HincII	GTYRAC	1 cut(s)	1657
HindII	GTYRAC	1 cut(s)	1657
HindIII	AAGCTT	2 cut(s)	563, 1484
HinfI	GANTC	9 cut(s)	158, 429, 857, 901, 1132, 1231, 1516, 1662, 1741
HpaI	GTTAAC	1 cut(s)	1657
HpaII	CCGG	4 cut(s)	138, 168, 891, 1604
HphI	GGTGA	4 cut(s)	137, 905, 967, 1025
Hpy166II	GTNNAC	4 cut(s)	713, 883, 1381, 1657
Hpy188I	TCNGA	6 cut(s)	304, 1236, 1314, 1339, 1618, 1746
Hpy188III	TCNNGA	5 cut(s)	392, 1049, 1300, 1423, 1604
Hpy8I	GTNNAC	4 cut(s)	713, 883, 1381, 1657
Hpy99I	CGWCG	2 cut(s)	56, 290
HpyAV	CCTTC	4 cut(s)	86, 913, 932, 946
HpyCH4III	ACNGT	2 cut(s)	236, 1363
HpyCH4V	TGCA	7 cut(s)	4, 335, 487, 1129, 1270, 1354, 1359
HpyF10VI	GCNNNNNNNGC	3 cut(s)	590, 636, 1259
Hsp92II	CATG	5 cut(s)	487, 1270, 1354, 1599, 1646
HspAI	GCGC	1 cut(s)	908
Kpn2I	TCCGGA	1 cut(s)	1603
KpnI	GGTACC	1 cut(s)	212
KroI	GCCGGC	1 cut(s)	167
KroNI	GCCGGC	1 cut(s)	169
Ksp22I	TGATCA	3 cut(s)	127, 463, 480
KspAI	GTTAAC	1 cut(s)	1657
LmnI	GCTCC	2 cut(s)	533, 636
Lsp1109I	GCAGC	3 cut(s)	536, 577, 792
LweI	GCATC	1 cut(s)	1212
MaeI	CTAG	6 cut(s)	426, 525, 672, 834, 911, 983
MaeIII	GTNAC	5 cut(s)	71, 263, 870, 955, 1582
MbiI	CCGCTC	1 cut(s)	103
MboII	GAAGA	9 cut(s)	101, 126, 404, 1064, 1301, 1340, 1429, 1540, 1750
MfeI	CAATTG	3 cut(s)	11, 57, 111
MflI	RGATCY	1 cut(s)	1419
MhlI	GDGCHC	1 cut(s)	1215
MlyI	GAGTC	4 cut(s)	438, 895, 1240, 1671
Mph1103I	ATGCAT	1 cut(s)	1356
MroI	TCCGGA	1 cut(s)	1603
MroNI	GCCGGC	1 cut(s)	167
MroXI	GAANNNNTTC	1 cut(s)	96
MseI	TTAA	8 cut(s)	77, 375, 567, 653, 819, 1457, 1656, 1667
MspA1I	CMGCKG	1 cut(s)	1492
MspCI	CTTAAG	1 cut(s)	76
MspI	CCGG	4 cut(s)	138, 168, 891, 1604
MspR9I	CCNGG	1 cut(s)	417
MunI	CAATTG	3 cut(s)	11, 57, 111
Mva1269I	GAATGC	1 cut(s)	1395
MvaI	CCWGG	1 cut(s)	417
MwoI	GCNNNNNNNGC	3 cut(s)	590, 636, 1259
NaeI	GCCGGC	1 cut(s)	169
NgoMIV	GCCGGC	1 cut(s)	167
NlaIII	CATG	5 cut(s)	487, 1270, 1354, 1599, 1646
NlaIV	GGNNCC	3 cut(s)	210, 933, 1004
NmuCI	GTSAC	2 cut(s)	870, 955
NsiI	ATGCAT	1 cut(s)	1356
NspI	RCATGY	2 cut(s)	1354, 1599
PciI	ACATGT	1 cut(s)	1595
PctI	GAATGC	1 cut(s)	1395
PdiI	GCCGGC	1 cut(s)	169
PdmI	GAANNNNTTC	1 cut(s)	96
PfeI	GAWTC	5 cut(s)	158, 857, 1132, 1516, 1741
PflFI	GACNNNGTC	1 cut(s)	968
PinAI	ACCGGT	1 cut(s)	890
PkrI	GCNGC	6 cut(s)	551, 592, 745, 766, 782, 1762
PleI	GAGTC	4 cut(s)	437, 895, 1239, 1670
PpsI	GAGTC	4 cut(s)	437, 895, 1239, 1670
PscI	ACATGT	1 cut(s)	1595
PshAI	GACNNNNGTC	1 cut(s)	404
PsiI	TTATAA	2 cut(s)	218, 1482
Psp124BI	GAGCTC	1 cut(s)	1215
Psp6I	CCWGG	1 cut(s)	415
PspFI	CCCAGC	1 cut(s)	1684
PspGI	CCWGG	1 cut(s)	415
PspN4I	GGNNCC	3 cut(s)	210, 933, 1004
PsuI	RGATCY	1 cut(s)	1419
PsyI	GACNNNGTC	1 cut(s)	968
PvuII	CAGCTG	1 cut(s)	1492
RsaI	GTAC	4 cut(s)	202, 210, 283, 1610
RsaNI	GTAC	4 cut(s)	201, 209, 282, 1609
SacI	GAGCTC	1 cut(s)	1215
SaqAI	TTAA	8 cut(s)	77, 375, 567, 653, 819, 1457, 1656, 1667
SatI	GCNGC	6 cut(s)	550, 591, 744, 765, 781, 1761
SchI	GAGTC	4 cut(s)	438, 895, 1240, 1671
ScrFI	CCNGG	1 cut(s)	417
SduI	GDGCHC	1 cut(s)	1215
SfaNI	GCATC	1 cut(s)	1212
SfcI	CTRYAG	1 cut(s)	1698
SmlI	CTYRAG	2 cut(s)	76, 947
SmoI	CTYRAG	2 cut(s)	76, 947
SsiI	CCGC	8 cut(s)	101, 744, 765, 1006, 1497, 1681, 1760, 1780
SspI	AATATT	3 cut(s)	298, 457, 976
SspMI	CTAG	6 cut(s)	426, 525, 672, 834, 911, 983
SstI	GAGCTC	1 cut(s)	1215
StyD4I	CCNGG	1 cut(s)	415
TaaI	ACNGT	2 cut(s)	236, 1363
TaqI	TCGA	7 cut(s)	51, 408, 860, 924, 1086, 1526, 1544
TatI	WGTACW	1 cut(s)	200
TauI	GCSGC	3 cut(s)	746, 767, 1763
TfiI	GAWTC	5 cut(s)	158, 857, 1132, 1516, 1741
Tru1I	TTAA	8 cut(s)	77, 375, 567, 653, 819, 1457, 1656, 1667
Tru9I	TTAA	8 cut(s)	77, 375, 567, 653, 819, 1457, 1656, 1667
TscAI	CASTG	2 cut(s)	1366, 1458
TseFI	GTSAC	2 cut(s)	870, 955
TseI	GCWGC	3 cut(s)	549, 590, 780
Tsp45I	GTSAC	2 cut(s)	870, 955
TspDTI	ATGAA	5 cut(s)	671, 932, 1032, 1255, 1547
TspGWI	ACGGA	6 cut(s)	28, 649, 1231, 1398, 1574, 1674
TspRI	CASTG	2 cut(s)	1366, 1458
Tth111I	GACNNNGTC	1 cut(s)	968
Vha464I	CTTAAG	1 cut(s)	76
XapI	RAATTY	5 cut(s)	1156, 1195, 1304, 1322, 1386
XceI	RCATGY	2 cut(s)	1354, 1599
XcmI	CCANNNNNNNNNTGG	1 cut(s)	115
XmnI	GAANNNNTTC	1 cut(s)	96
XspI	CTAG	6 cut(s)	426, 525, 672, 834, 911, 983
Zsp2I	ATGCAT	1 cut(s)	1356

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.