Prupe.1G022200_v2.0.a1

Perakine reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0029160
Physical Location & Seq
Reverse (-)
1518144 .. 1519835
1692 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G022200.1_v2.0.a1.CDS.5

Sequence Viewer

Length: 894 bp
ATGGAGGGCAAGCCCCAAGTTCAAATTCCAACAGTTAAACTGGGTAGTCAGGGCTTAGAGGTTTCTAGATTGGGCTATGGATACAGCGGGCTATCTGGAAAATACAACTCTCCTCTCTCTCATGAGGCTGGATGTTCAGTTATCAAGGAAGCATTTGAGAGGGGTATCACCTTCTTTGATACTTCAGATATTTATGGCCAAAATCATGATAATGAAATCTTGGTTGGCAAGGGGACCCCAGAATATGTAAGAGCATGTTGTGAAGCTAGTCTTAAACGGCTGGCTGTCAGCTACATTGATCTGTACTATCAGCACCGTGTGGATGTTTCAGTGCCAATTGAGGATACTATAGAGGAGCTTAAGAAGCTGGTAAATGAAGGAAAGATAAGATACATTGGTCTATCAGAAGCCAGTGTTGACACAATAAGTAGAGCTCATGCTGTTCATCCCATCACTGCTGTACAAATGGAGTATTCTCTGTGGAGTCGAGAAATCGAAAACGAGATAACTCAACTTTGCAGGAAGCTTGGAATTGGGATCGTGTCATATAGCCCGCTTGGTCGTGGGCTCTTTGGTGGGAAGGCAGTTGTAGAGAGCTTGCCTGCAGACTGTTTGTTGTCTAAACATCCAAGGTTCAATGGGGAGAACTTGGAAAAGAACAAACTTCTCTACAGTAAACTTGCCACCCTTGCTGCAAAGCATGCATGCACTGCTCCACAATTAGCTCTGGCGTGGCTACTTCACCATGGAAATGACAACATCCCAATCCCCGGGACGACTAAAGTTAAGAACCTTGATACCATCGTTGGATCATTGGATGTGGAGCTTACAAAAGAGGATCTGAAAGAAATTTGTGATGCTGTGAAGATTGATGAAGTTAGGGGCGACCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

298

Amino Acids

32.69

Weight (kDa)

6.02

Isoelectric Point (pI)

25.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029160)

Species Orthologous Gene IDs
prunus_persica Prupe.1G022200_v2.0.a1 Prupe.1G022200_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 87, 554
AclWI GGATC 3 cut(s) 545, 817, 846
AcoI YGGCCR 1 cut(s) 196
AcsI RAATTY 2 cut(s) 24, 849
AcuI CTGAAG 1 cut(s) 168
AdeI CACNNNGTG 1 cut(s) 319
AfaI GTAC 2 cut(s) 305, 462
AfiI CCNNNNNNNGG 1 cut(s) 770
AflII CTTAAG 1 cut(s) 359
AgsI TTSAA 2 cut(s) 23, 637
AjuI GAANNNNNNNTTGG 4 cut(s) 9, 41, 207, 239
AluBI AGCT 9 cut(s) 266, 291, 358, 367, 434, 526, 597, 725, 826
AluI AGCT 9 cut(s) 266, 291, 358, 367, 434, 526, 597, 725, 826
Alw21I GWGCWC 1 cut(s) 436
AlwI GGATC 3 cut(s) 545, 817, 846
Ama87I CYCGRG 1 cut(s) 770
AoxI GGCC 1 cut(s) 196
ApeKI GCWGC 1 cut(s) 692
ApoI RAATTY 2 cut(s) 24, 849
AspS9I GGNCC 1 cut(s) 234
AsuC2I CCSGG 2 cut(s) 771, 772
AsuHPI GGTGA 2 cut(s) 160, 734
AvaI CYCGRG 1 cut(s) 770
AvaII GGWCC 1 cut(s) 234
BaeI ACNNNNGTAYC 4 cut(s) 382, 415, 789, 822
BalI TGGCCA 1 cut(s) 198
BanII GRGCYC 2 cut(s) 436, 570
Bbv12I GWGCWC 1 cut(s) 436
BbvI GCAGC 1 cut(s) 679
BccI CCATC 2 cut(s) 458, 809
BceAI ACGGC 1 cut(s) 293
BciVI GTATCC 2 cut(s) 74, 337
BcnI CCSGG 2 cut(s) 771, 772
BfaI CTAG 2 cut(s) 66, 267
BfmI CTRYAG 3 cut(s) 348, 603, 670
BfrI CTTAAG 1 cut(s) 359
BfuI GTATCC 2 cut(s) 74, 337
BisI GCNGC 1 cut(s) 693
BlsI GCNGC 1 cut(s) 694
Bme1390I CCNGG 2 cut(s) 771, 772
Bme18I GGWCC 1 cut(s) 234
BmeT110I CYCGRG 1 cut(s) 770
BmgT120I GGNCC 1 cut(s) 234
BmiI GGNNCC 2 cut(s) 235, 236
BmrFI CCNGG 2 cut(s) 771, 772
BmrI ACTGGG 1 cut(s) 50
BmsI GCATC 1 cut(s) 847
BmuI ACTGGG 1 cut(s) 50
BpuMI CCSGG 2 cut(s) 771, 772
BsaJI CCNNGG 4 cut(s) 629, 745, 769, 770
Bsc4I CCNNNNNNNGG 1 cut(s) 770
Bse1I ACTGG 2 cut(s) 45, 411
BseDI CCNNGG 4 cut(s) 629, 745, 769, 770
BseGI GGATG 6 cut(s) 137, 328, 445, 625, 759, 823
BseLI CCNNNNNNNGG 1 cut(s) 770
BseNI ACTGG 2 cut(s) 45, 411
BseRI GAGGAG 2 cut(s) 102, 368
BseXI GCAGC 1 cut(s) 679
Bsh1285I CGRYCG 1 cut(s) 889
BshFI GGCC 1 cut(s) 198
BsiEI CGRYCG 1 cut(s) 889
BsiHKAI GWGCWC 1 cut(s) 436
BsiHKCI CYCGRG 1 cut(s) 770
BsiSI CCGG 1 cut(s) 771
BslFI GGGAC 2 cut(s) 247, 787
BslI CCNNNNNNNGG 1 cut(s) 770
BsmFI GGGAC 2 cut(s) 247, 787
BsnI GGCC 1 cut(s) 198
BsoBI CYCGRG 1 cut(s) 770
Bsp1286I GDGCHC 2 cut(s) 436, 570
Bsp1407I TGTACA 1 cut(s) 460
Bsp143I GATC 4 cut(s) 298, 537, 809, 838
Bsp19I CCATGG 1 cut(s) 745
BspACI CCGC 2 cut(s) 87, 554
BspANI GGCC 1 cut(s) 198
BspHI TCATGA 2 cut(s) 121, 205
BspLI GGNNCC 2 cut(s) 235, 236
BspMAI CTGCAG 1 cut(s) 607
BspPI GGATC 3 cut(s) 545, 817, 846
BspTI CTTAAG 1 cut(s) 359
BsrGI TGTACA 1 cut(s) 460
BsrI ACTGG 2 cut(s) 45, 411
BssECI CCNNGG 4 cut(s) 629, 745, 769, 770
BssMI GATC 4 cut(s) 298, 537, 809, 838
BssT1I CCWWGG 2 cut(s) 629, 745
Bst4CI ACNGT 4 cut(s) 34, 317, 611, 674
BstAFI CTTAAG 1 cut(s) 359
BstAPI GCANNNNNTGC 2 cut(s) 701, 710
BstAUI TGTACA 1 cut(s) 460
BstC8I GCNNGC 8 cut(s) 11, 89, 282, 554, 599, 603, 702, 706
BstDEI CTNAG 1 cut(s) 55
BstDSI CCRYGG 1 cut(s) 745
BstF5I GGATG 6 cut(s) 137, 328, 445, 625, 759, 823
BstKTI GATC 4 cut(s) 301, 540, 812, 841
BstMBI GATC 4 cut(s) 298, 537, 809, 838
BstMCI CGRYCG 1 cut(s) 889
BstMWI GCNNNNNNNGC 4 cut(s) 364, 689, 701, 710
BstNSI RCATGY 3 cut(s) 258, 704, 708
BstSCI CCNGG 2 cut(s) 769, 770
BstSFI CTRYAG 3 cut(s) 348, 603, 670
BstV1I GCAGC 1 cut(s) 679
BstX2I RGATCY 1 cut(s) 838
BstYI RGATCY 1 cut(s) 838
BsuI GTATCC 2 cut(s) 74, 337
BsuRI GGCC 1 cut(s) 198
BtgI CCRYGG 1 cut(s) 745
BtsCI GGATG 6 cut(s) 137, 328, 445, 625, 759, 823
BtsI GCAGTG 2 cut(s) 453, 708
BtsIMutI CAGTG 4 cut(s) 336, 418, 453, 708
Cac8I GCNNGC 8 cut(s) 11, 89, 282, 554, 599, 603, 702, 706
CciI TCATGA 2 cut(s) 121, 205
Cfr13I GGNCC 1 cut(s) 234
Cfr9I CCCGGG 1 cut(s) 770
Csp6I GTAC 2 cut(s) 304, 461
CviAII CATG 7 cut(s) 122, 206, 255, 437, 701, 705, 746
CviQI GTAC 2 cut(s) 304, 461
DdeI CTNAG 1 cut(s) 55
DpnI GATC 4 cut(s) 300, 539, 811, 840
DpnII GATC 4 cut(s) 298, 537, 809, 838
DraIII CACNNNGTG 1 cut(s) 319
EaeI YGGCCR 1 cut(s) 196
Ecl136II GAGCTC 1 cut(s) 434
Eco130I CCWWGG 2 cut(s) 629, 745
Eco24I GRGCYC 2 cut(s) 436, 570
Eco47I GGWCC 1 cut(s) 234
Eco53kI GAGCTC 1 cut(s) 434
Eco57I CTGAAG 1 cut(s) 168
Eco88I CYCGRG 1 cut(s) 770
EcoICRI GAGCTC 1 cut(s) 434
EcoO109I RGGNCCY 1 cut(s) 234
EcoT14I CCWWGG 2 cut(s) 629, 745
EcoT22I ATGCAT 1 cut(s) 706
EcoT38I GRGCYC 2 cut(s) 436, 570
ErhI CCWWGG 2 cut(s) 629, 745
FaeI CATG 7 cut(s) 125, 209, 258, 440, 704, 708, 749
FalI AAGNNNNNCTT 2 cut(s) 255, 287
FaqI GGGAC 2 cut(s) 247, 787
FatI CATG 7 cut(s) 121, 205, 254, 436, 700, 704, 745
FauI CCCGC 2 cut(s) 80, 561
Fnu4HI GCNGC 1 cut(s) 693
FokI GGATG 6 cut(s) 144, 335, 432, 612, 746, 830
FriOI GRGCYC 2 cut(s) 436, 570
Fsp4HI GCNGC 1 cut(s) 693
FspBI CTAG 2 cut(s) 66, 267
GluI GCNGC 1 cut(s) 693
HaeIII GGCC 1 cut(s) 198
HapII CCGG 1 cut(s) 771
Hin1II CATG 7 cut(s) 125, 209, 258, 440, 704, 708, 749
HincII GTYRAC 1 cut(s) 418
HindII GTYRAC 1 cut(s) 418
HindIII AAGCTT 1 cut(s) 524
HinfI GANTC 1 cut(s) 484
HpaII CCGG 1 cut(s) 771
HphI GGTGA 2 cut(s) 160, 734
Hpy166II GTNNAC 2 cut(s) 418, 677
Hpy188I TCNGA 3 cut(s) 187, 406, 843
Hpy188III TCNNGA 5 cut(s) 66, 96, 122, 206, 488
Hpy8I GTNNAC 2 cut(s) 418, 677
HpyAV CCTTC 3 cut(s) 181, 371, 574
HpyCH4III ACNGT 4 cut(s) 34, 317, 611, 674
HpyCH4V TGCA 5 cut(s) 519, 605, 695, 704, 708
HpyF10VI GCNNNNNNNGC 4 cut(s) 364, 689, 701, 710
HpyF3I CTNAG 1 cut(s) 55
Hsp92II CATG 7 cut(s) 125, 209, 258, 440, 704, 708, 749
KflI GGGWCCC 1 cut(s) 234
Kzo9I GATC 4 cut(s) 298, 537, 809, 838
LmnI GCTCC 3 cut(s) 355, 718, 823
Lsp1109I GCAGC 1 cut(s) 679
LweI GCATC 1 cut(s) 847
MaeI CTAG 2 cut(s) 66, 267
MalI GATC 4 cut(s) 300, 539, 811, 840
MboI GATC 4 cut(s) 298, 537, 809, 838
MboII GAAGA 1 cut(s) 877
MfeI CAATTG 1 cut(s) 336
MflI RGATCY 1 cut(s) 838
MhlI GDGCHC 2 cut(s) 436, 570
MlsI TGGCCA 1 cut(s) 198
MluCI AATT 5 cut(s) 24, 336, 531, 719, 849
MluNI TGGCCA 1 cut(s) 198
MlyI GAGTC 1 cut(s) 493
MmeI TCCRAC 2 cut(s) 53, 787
MnlI CCTC 7 cut(s) 52, 118, 123, 153, 334, 346, 829
Mox20I TGGCCA 1 cut(s) 198
Mph1103I ATGCAT 1 cut(s) 706
MscI TGGCCA 1 cut(s) 198
MseI TTAA 4 cut(s) 36, 273, 360, 786
MslI CAYNNNNRTG 2 cut(s) 210, 750
Msp20I TGGCCA 1 cut(s) 198
MspA1I CMGCKG 1 cut(s) 87
MspCI CTTAAG 1 cut(s) 359
MspI CCGG 1 cut(s) 771
MspR9I CCNGG 2 cut(s) 771, 772
MunI CAATTG 1 cut(s) 336
MwoI GCNNNNNNNGC 4 cut(s) 364, 689, 701, 710
NciI CCSGG 2 cut(s) 771, 772
NcoI CCATGG 1 cut(s) 745
NdeII GATC 4 cut(s) 298, 537, 809, 838
NlaIII CATG 7 cut(s) 125, 209, 258, 440, 704, 708, 749
NlaIV GGNNCC 2 cut(s) 235, 236
NsiI ATGCAT 1 cut(s) 706
NspI RCATGY 3 cut(s) 258, 704, 708
PaeI GCATGC 2 cut(s) 704, 708
PagI TCATGA 2 cut(s) 121, 205
PkrI GCNGC 1 cut(s) 694
PleI GAGTC 1 cut(s) 492
PpsI GAGTC 1 cut(s) 492
PpuMI RGGWCCY 1 cut(s) 234
Psp124BI GAGCTC 1 cut(s) 436
Psp5II RGGWCCY 1 cut(s) 234
PspN4I GGNNCC 2 cut(s) 235, 236
PspPI GGNCC 1 cut(s) 234
PspPPI RGGWCCY 1 cut(s) 234
PstI CTGCAG 1 cut(s) 607
PsuI RGATCY 1 cut(s) 838
RsaI GTAC 2 cut(s) 305, 462
RsaNI GTAC 2 cut(s) 304, 461
RseI CAYNNNNRTG 2 cut(s) 210, 750
SacI GAGCTC 1 cut(s) 436
SaqAI TTAA 4 cut(s) 36, 273, 360, 786
SatI GCNGC 1 cut(s) 693
Sau3AI GATC 4 cut(s) 298, 537, 809, 838
Sau96I GGNCC 1 cut(s) 234
SchI GAGTC 1 cut(s) 493
ScrFI CCNGG 2 cut(s) 771, 772
SduI GDGCHC 2 cut(s) 436, 570
SfaNI GCATC 1 cut(s) 847
SfcI CTRYAG 3 cut(s) 348, 603, 670
SinI GGWCC 1 cut(s) 234
SmaI CCCGGG 1 cut(s) 772
SmiMI CAYNNNNRTG 2 cut(s) 210, 750
SmlI CTYRAG 1 cut(s) 359
SmoI CTYRAG 1 cut(s) 359
SphI GCATGC 2 cut(s) 704, 708
Sse9I AATT 5 cut(s) 24, 336, 531, 719, 849
SsiI CCGC 2 cut(s) 87, 554
SspMI CTAG 2 cut(s) 66, 267
SstI GAGCTC 1 cut(s) 436
StyD4I CCNGG 2 cut(s) 769, 770
StyI CCWWGG 2 cut(s) 629, 745
TaaI ACNGT 4 cut(s) 34, 317, 611, 674
TaqI TCGA 2 cut(s) 487, 495
TasI AATT 5 cut(s) 24, 336, 531, 719, 849
TatI WGTACW 2 cut(s) 303, 460
Tru1I TTAA 4 cut(s) 36, 273, 360, 786
Tru9I TTAA 4 cut(s) 36, 273, 360, 786
TscAI CASTG 4 cut(s) 336, 418, 460, 715
TseI GCWGC 1 cut(s) 692
TspDTI ATGAA 4 cut(s) 228, 390, 434, 888
TspMI CCCGGG 1 cut(s) 770
TspRI CASTG 4 cut(s) 336, 418, 460, 715
Vha464I CTTAAG 1 cut(s) 359
VpaK11BI GGWCC 1 cut(s) 234
XapI RAATTY 2 cut(s) 24, 849
XbaI TCTAGA 1 cut(s) 65
XceI RCATGY 3 cut(s) 258, 704, 708
XmaI CCCGGG 1 cut(s) 770
XspI CTAG 2 cut(s) 66, 267
Zsp2I ATGCAT 1 cut(s) 706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.