Prupe.1G070400_v2.0.a1

RNA helicase SDE3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
N/A
Physical Location & Seq
Forward (+)
4994699 .. 4999583
4885 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G070400.1_v2.0.a1.CDS.3

Sequence Viewer

Length: 1914 bp
ATGACTCACTCTTCCATTTCTGACCAAGTTATTATCTTCCCCTCTAAGCATGAGAGAAATGAAATGGCCTGCAATCAAGAAACAAAACCGGAGTTGTTGGAACACCGTGGGCCCCTTTTGCTGATTTGGCAAGACCCGGTTAGGGGTGGGGCTGGTGAGCGTGTACATTATTATGGAAGAATCTTCAGTCTTCAGACTTTTCAACTTTCCAAAACCAAAAGGAAAGCGACGTTGAGTCGTCGGCTGGTCAACACTCAGCAACAAGCTCAGCTGCTGCTGCATTGCATTCGAAAGAAGAAAAGCATGTCATTTTTTCGTAAACTCTTGAAAGGGCTTCTTGGTGGTGAAGATGAGCATGAATGTTATTATGGTGAAGATGTGCCTGAATTTTATTATGGTTGTTACAATCAGCTTCCCGAATTACACTATCGTGATTATCGTTACGATAATTCCCACAGATACCTCTTGCCACCCCCTAAACCCCCATCAACTCCATCTAAACCATCCTGTCTCCCTTTAATCCACCCTTCATCTTCCCCTAAACCTCCTACATTGTGTAGAAAACCGCAATCTTCTTCCCTCGATACATCTTCGTCATCTCCTAAATTCGTAACGTCTTTATCTAAACCACTTCGGGCTTCATTATTTAGGCCACGTCCTTCTTCATCTAAGCCACATCCATTATCCCCAAAACCTCCAACATCGTCTTCAAGACCATCTCCATCTTCCCCTAATAGAACTCCATTTTCCTCTGGACCACCCTTTTCTTCAGATAAGCTCCCTCCAATCTTTAAGACATTTCTATCCCCCGGGAAGAAAAATTATGTATGGGTTGACAAGGGTTCATTACCTATATTCATGATTCCTGGCGATATCAAAGATTTGATCAAGAATGACATTGTGCCTAAAGTTCTTAACCAGCCTCTGTCTCTCACGACATACAAGGATTACTTTGCTGTTCTGTTATATGCTGAAGAGTTCTACTTTGAGCGTATCGAGGAGCAGTCTTTGATCGAGGGGCTTTATATATCGAGGGGATTTGAATTTGAAGATGACTTCTACGTTCATCATGATTCGACTCAAAAATATGATGTCAGCTTCTCATTCAATAGAGTCTGTTTGAAACGAGCTTACCAAGCAGTTCAATATGCATCAGATGCTTTGTTTCGGAACTTCCTCTTTCCCGATTGTATTTCCCCGCTGCTCTCTGCCTGTCATAAACTTGATGCTGATCAATTATCTGCAGTTCGCCATATTTTAAGCATTCAGGGCTCACCACCTTACTTGGTGGCTGGCCAGCTATGTGTCGAAAGAAATGCTTTTTGGCCATCCAAAACTGGAGTGGTTGTTTGTGAAGCAGTGCACCAGTTATGTCAAATGTCAACAGAAAATAGGATTCTTATACGTGCTCATAATAACCACTGTTGTGATGTGCTCATGAGGAGCTTGTTGAAAGTGATTCCAGAGTCAGATATGTTTCGAGCCAATGCTGCATTCCGTGAGATAGATGGGGTACCTGAAGACATCCTCCCATCATGCCTTTACAAAGAGCCTTATTTCTCTTGTCCTCCAACTGAGGAGCTTAAAAAATTCAGGGTGATTTTCTCGACTTTCACGAGTAGCTTTCGGCTACATGACGAAGGCCTAAATGCAGGACATTTCAGTCATATTTTTCTGGTGGATGCTTCGTCTGCTATCGAGCCAGAAACAGTAGTGGCCCTGACTTTTGCTGACAAGAATACTACTGTTATAGTTACTGGTGAACGAGGAAACCGTTCACATTGGGTTCGAGCTGACATTGCAAGGGAAAAGGGGCTGAAGATTTCATTGCCATATAGAAGCCTCAATCCAATGTTCATCACGCAACTGGATCTGCATAGCAAGAGTCGGACAACATCCAAAGGCTATAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

638

Amino Acids

72.46

Weight (kDa)

9.0

Isoelectric Point (pI)

55.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1662
Acc65I GGTACC 1 cut(s) 1513
AccB1I GGYRCC 1 cut(s) 1513
AciI CCGC 2 cut(s) 566, 1199
AclWI GGATC 1 cut(s) 1878
AcoI YGGCCR 2 cut(s) 1294, 1325
AcsI RAATTY 4 cut(s) 386, 605, 1043, 1589
AcuI CTGAAG 6 cut(s) 169, 176, 753, 993, 1539, 1838
AfaI GTAC 2 cut(s) 165, 1515
AfiI CCNNNNNNNGG 2 cut(s) 142, 143
AgsI TTSAA 9 cut(s) 203, 328, 711, 1043, 1049, 1108, 1123, 1145, 1453
AhdI GACNNNNNGTC 1 cut(s) 234
AjiI CACGTC 1 cut(s) 656
AjnI CCWGG 1 cut(s) 865
AjuI GAANNNNNNNTTGG 6 cut(s) 691, 723, 1306, 1338, 1478, 1510
AleI CACNNNNGTG 2 cut(s) 429, 1425
Alw21I GWGCWC 3 cut(s) 1365, 1411, 1437
Alw26I GTCTC 2 cut(s) 515, 933
Alw44I GTGCAC 1 cut(s) 1361
AlwI GGATC 1 cut(s) 1878
AlwNI CAGNNNCTG 2 cut(s) 274, 925
Ama87I CYCGRG 1 cut(s) 809
AoxI GGCC 7 cut(s) 66, 110, 650, 1294, 1325, 1642, 1716
ApaI GGGCCC 1 cut(s) 114
ApaLI GTGCAC 1 cut(s) 1361
ApeKI GCWGC 5 cut(s) 271, 274, 277, 1201, 1490
ApoI RAATTY 4 cut(s) 386, 605, 1043, 1589
Asp700I GAANNNNTTC 1 cut(s) 1774
Asp718I GGTACC 1 cut(s) 1513
AspS9I GGNCC 4 cut(s) 110, 111, 755, 1717
AsuC2I CCSGG 3 cut(s) 137, 810, 811
AsuHPI GGTGA 6 cut(s) 167, 356, 383, 1266, 1609, 1772
AsuII TTCGAA 1 cut(s) 289
AvaI CYCGRG 1 cut(s) 809
AvaII GGWCC 1 cut(s) 755
BaeGI GKGCMC 2 cut(s) 114, 1365
BalI TGGCCA 2 cut(s) 1296, 1327
BanI GGYRCC 1 cut(s) 1513
BanII GRGCYC 2 cut(s) 114, 1274
BauI CACGAG 1 cut(s) 1615
BbsI GAAGAC 3 cut(s) 182, 699, 1527
Bbv12I GWGCWC 3 cut(s) 1365, 1411, 1437
BbvI GCAGC 5 cut(s) 258, 261, 264, 1188, 1477
BccI CCATC 8 cut(s) 493, 502, 511, 724, 730, 1336, 1502, 1540
BcgI CGANNNNNNTGC 4 cut(s) 269, 303, 1298, 1332
BciT130I CCWGG 1 cut(s) 867
BclI TGATCA 2 cut(s) 885, 1231
BcnI CCSGG 3 cut(s) 137, 810, 811
BcoDI GTCTC 2 cut(s) 515, 933
BfaI CTAG 1 cut(s) 1912
BfmI CTRYAG 1 cut(s) 1242
BisI GCNGC 5 cut(s) 272, 275, 278, 1202, 1491
BlpI GCTNAGC 1 cut(s) 267
BlsI GCNGC 5 cut(s) 273, 276, 279, 1203, 1492
Bme1390I CCNGG 4 cut(s) 137, 810, 811, 867
Bme18I GGWCC 1 cut(s) 755
BmeRI GACNNNNNGTC 1 cut(s) 234
BmeT110I CYCGRG 1 cut(s) 809
BmgBI CACGTC 1 cut(s) 656
BmgT120I GGNCC 4 cut(s) 110, 111, 755, 1717
BmiI GGNNCC 3 cut(s) 112, 113, 1515
BmrFI CCNGG 4 cut(s) 137, 810, 811, 867
BmsI GCATC 4 cut(s) 1147, 1160, 1216, 1672
BpiI GAAGAC 3 cut(s) 182, 699, 1527
BpmI CTGGAG 1 cut(s) 1359
Bpu1102I GCTNAGC 1 cut(s) 267
Bpu14I TTCGAA 1 cut(s) 289
BpuMI CCSGG 3 cut(s) 137, 810, 811
BsaAI YACGTR 1 cut(s) 1406
BsaBI GATNNNNATC 1 cut(s) 1230
BsaJI CCNNGG 3 cut(s) 106, 808, 809
BsaWI WCCGGW 1 cut(s) 88
BsaXI ACNNNNNCTCC 2 cut(s) 1332, 1362
Bsc4I CCNNNNNNNGG 2 cut(s) 142, 143
Bse1I ACTGG 4 cut(s) 1342, 1366, 1762, 1872
Bse3DI GCAATG 3 cut(s) 280, 1797, 1826
Bse8I GATNNNNATC 1 cut(s) 1230
BseBI CCWGG 1 cut(s) 867
BseDI CCNNGG 3 cut(s) 106, 808, 809
BseGI GGATG 6 cut(s) 503, 676, 1328, 1524, 1687, 1895
BseJI GATNNNNATC 1 cut(s) 1230
BseLI CCNNNNNNNGG 2 cut(s) 142, 143
BseMI GCAATG 3 cut(s) 280, 1797, 1826
BseMII CTCAG 3 cut(s) 269, 281, 1566
BseNI ACTGG 4 cut(s) 1342, 1366, 1762, 1872
BseRI GAGGAG 3 cut(s) 1013, 1456, 1592
BseSI GKGCMC 2 cut(s) 114, 1365
BseXI GCAGC 5 cut(s) 258, 261, 264, 1188, 1477
BshFI GGCC 7 cut(s) 68, 112, 652, 1296, 1327, 1644, 1718
BshNI GGYRCC 1 cut(s) 1513
BsiHKAI GWGCWC 3 cut(s) 1365, 1411, 1437
BsiHKCI CYCGRG 1 cut(s) 809
BsiSI CCGG 3 cut(s) 89, 137, 810
BslI CCNNNNNNNGG 2 cut(s) 142, 143
BsmAI GTCTC 2 cut(s) 515, 933
BsmI GAATGC 3 cut(s) 285, 1263, 1493
BsnI GGCC 7 cut(s) 68, 112, 652, 1296, 1327, 1644, 1718
BsoBI CYCGRG 1 cut(s) 809
Bsp119I TTCGAA 1 cut(s) 289
Bsp120I GGGCCC 1 cut(s) 110
Bsp1286I GDGCHC 5 cut(s) 114, 1274, 1365, 1411, 1437
Bsp1407I TGTACA 1 cut(s) 163
Bsp143I GATC 4 cut(s) 885, 1011, 1231, 1870
Bsp1720I GCTNAGC 1 cut(s) 267
BspACI CCGC 2 cut(s) 566, 1199
BspANI GGCC 7 cut(s) 68, 112, 652, 1296, 1327, 1644, 1718
BspCNI CTCAG 3 cut(s) 268, 280, 1567
BspHI TCATGA 3 cut(s) 858, 1069, 1437
BspLI GGNNCC 3 cut(s) 112, 113, 1515
BspMAI CTGCAG 1 cut(s) 1246
BspPI GGATC 1 cut(s) 1878
BspT104I TTCGAA 1 cut(s) 289
BspT107I GGYRCC 1 cut(s) 1513
BsrDI GCAATG 3 cut(s) 280, 1797, 1826
BsrGI TGTACA 1 cut(s) 163
BsrI ACTGG 4 cut(s) 1342, 1366, 1762, 1872
BssECI CCNNGG 3 cut(s) 106, 808, 809
BssMI GATC 4 cut(s) 885, 1011, 1231, 1870
BssSI CACGAG 1 cut(s) 1615
Bst2BI CACGAG 1 cut(s) 1615
Bst2UI CCWGG 1 cut(s) 867
Bst4CI ACNGT 5 cut(s) 107, 1424, 1711, 1747, 1775
Bst6I CTCTTC 2 cut(s) 16, 969
BstAPI GCANNNNNTGC 1 cut(s) 1157
BstAUI TGTACA 1 cut(s) 163
BstBAI YACGTR 1 cut(s) 1406
BstBI TTCGAA 1 cut(s) 289
BstC8I GCNNGC 3 cut(s) 70, 1294, 1298
BstDEI CTNAG 5 cut(s) 45, 255, 267, 669, 1575
BstDSI CCRYGG 1 cut(s) 106
BstF5I GGATG 6 cut(s) 503, 676, 1328, 1524, 1687, 1895
BstKTI GATC 4 cut(s) 888, 1014, 1234, 1873
BstMAI GTCTC 2 cut(s) 515, 933
BstMBI GATC 4 cut(s) 885, 1011, 1231, 1870
BstMWI GCNNNNNNNGC 9 cut(s) 118, 127, 277, 1136, 1157, 1269, 1490, 1691, 1799
BstNI CCWGG 1 cut(s) 867
BstNSI RCATGY 1 cut(s) 307
BstSCI CCNGG 4 cut(s) 135, 808, 809, 865
BstSFI CTRYAG 1 cut(s) 1242
BstSLI GKGCMC 2 cut(s) 114, 1365
BstV1I GCAGC 5 cut(s) 258, 261, 264, 1188, 1477
BstV2I GAAGAC 3 cut(s) 182, 699, 1527
BstX2I RGATCY 1 cut(s) 1870
BstYI RGATCY 1 cut(s) 1870
BsuRI GGCC 7 cut(s) 68, 112, 652, 1296, 1327, 1644, 1718
BtgI CCRYGG 1 cut(s) 106
BtrI CACGTC 1 cut(s) 656
BtsCI GGATG 6 cut(s) 503, 676, 1328, 1524, 1687, 1895
BtsI GCAGTG 1 cut(s) 1365
BtsIMutI CAGTG 2 cut(s) 1365, 1420
Cac8I GCNNGC 3 cut(s) 70, 1294, 1298
CaiI CAGNNNCTG 2 cut(s) 274, 925
CciI TCATGA 3 cut(s) 858, 1069, 1437
Cfr13I GGNCC 4 cut(s) 110, 111, 755, 1717
Cfr9I CCCGGG 1 cut(s) 809
Csp6I GTAC 2 cut(s) 164, 1514
CspCI CAANNNNNGTGG 2 cut(s) 1266, 1301
CviAII CATG 8 cut(s) 50, 304, 356, 859, 1070, 1438, 1536, 1634
CviQI GTAC 2 cut(s) 164, 1514
DdeI CTNAG 5 cut(s) 45, 255, 267, 669, 1575
DpnI GATC 4 cut(s) 887, 1013, 1233, 1872
DpnII GATC 4 cut(s) 885, 1011, 1231, 1870
DrdI GACNNNNNNGTC 1 cut(s) 1662
DriI GACNNNNNGTC 1 cut(s) 234
DseDI GACNNNNNNGTC 1 cut(s) 1662
EaeI YGGCCR 2 cut(s) 1294, 1325
Eam1104I CTCTTC 2 cut(s) 16, 969
Eam1105I GACNNNNNGTC 1 cut(s) 234
EarI CTCTTC 2 cut(s) 16, 969
Eco147I AGGCCT 1 cut(s) 1644
Eco24I GRGCYC 2 cut(s) 114, 1274
Eco32I GATATC 1 cut(s) 874
Eco47I GGWCC 1 cut(s) 755
Eco57I CTGAAG 6 cut(s) 169, 176, 753, 993, 1539, 1838
Eco88I CYCGRG 1 cut(s) 809
EcoO109I RGGNCCY 1 cut(s) 111
EcoRII CCWGG 1 cut(s) 865
EcoRV GATATC 1 cut(s) 874
EcoT22I ATGCAT 1 cut(s) 1153
EcoT38I GRGCYC 2 cut(s) 114, 1274
FaeI CATG 8 cut(s) 53, 307, 359, 862, 1073, 1441, 1539, 1637
FalI AAGNNNNNCTT 6 cut(s) 321, 353, 935, 967, 1303, 1335
FatI CATG 8 cut(s) 49, 303, 355, 858, 1069, 1437, 1535, 1633
FauI CCCGC 1 cut(s) 1206
FbaI TGATCA 2 cut(s) 885, 1231
Fnu4HI GCNGC 5 cut(s) 272, 275, 278, 1202, 1491
FokI GGATG 6 cut(s) 490, 663, 1315, 1511, 1694, 1882
FriOI GRGCYC 2 cut(s) 114, 1274
Fsp4HI GCNGC 5 cut(s) 272, 275, 278, 1202, 1491
FspBI CTAG 1 cut(s) 1912
GluI GCNGC 5 cut(s) 272, 275, 278, 1202, 1491
GsuI CTGGAG 1 cut(s) 1359
HaeIII GGCC 7 cut(s) 68, 112, 652, 1296, 1327, 1644, 1718
HapII CCGG 3 cut(s) 89, 137, 810
Hin1II CATG 8 cut(s) 53, 307, 359, 862, 1073, 1441, 1539, 1637
HincII GTYRAC 3 cut(s) 250, 835, 1383
HindII GTYRAC 3 cut(s) 250, 835, 1383
HpaII CCGG 3 cut(s) 89, 137, 810
HphI GGTGA 6 cut(s) 167, 356, 383, 1266, 1609, 1772
Hpy166II GTNNAC 8 cut(s) 164, 250, 320, 835, 1363, 1383, 1763, 1778
Hpy188I TCNGA 7 cut(s) 22, 195, 772, 1156, 1170, 1471, 1890
Hpy8I GTNNAC 8 cut(s) 164, 250, 320, 835, 1363, 1383, 1763, 1778
Hpy99I CGWCG 2 cut(s) 232, 243
HpyAV CCTTC 3 cut(s) 537, 669, 1634
HpyCH4III ACNGT 5 cut(s) 107, 1424, 1711, 1747, 1775
HpyCH4IV ACGT 5 cut(s) 230, 614, 655, 1062, 1405
HpyF10VI GCNNNNNNNGC 9 cut(s) 118, 127, 277, 1136, 1157, 1269, 1490, 1691, 1799
HpyF3I CTNAG 5 cut(s) 45, 255, 267, 669, 1575
HpySE526I ACGT 5 cut(s) 230, 614, 655, 1062, 1405
Hsp92II CATG 8 cut(s) 53, 307, 359, 862, 1073, 1441, 1539, 1637
KpnI GGTACC 1 cut(s) 1517
Ksp22I TGATCA 2 cut(s) 885, 1231
Kzo9I GATC 4 cut(s) 885, 1011, 1231, 1870
LmnI GCTCC 4 cut(s) 783, 1000, 1443, 1579
Lsp1109I GCAGC 5 cut(s) 258, 261, 264, 1188, 1477
LweI GCATC 4 cut(s) 1147, 1160, 1216, 1672
MaeI CTAG 1 cut(s) 1912
MaeII ACGT 5 cut(s) 230, 614, 655, 1062, 1405
MaeIII GTNAC 4 cut(s) 401, 440, 610, 1753
MalI GATC 4 cut(s) 887, 1013, 1233, 1872
MboI GATC 4 cut(s) 885, 1011, 1231, 1870
MflI RGATCY 1 cut(s) 1870
MhlI GDGCHC 5 cut(s) 114, 1274, 1365, 1411, 1437
MlsI TGGCCA 2 cut(s) 1296, 1327
MluCI AATT 8 cut(s) 386, 419, 448, 605, 820, 1043, 1235, 1589
MluNI TGGCCA 2 cut(s) 1296, 1327
MlyI GAGTC 5 cut(s) 244, 1072, 1122, 1475, 1894
MmeI TCCRAC 4 cut(s) 78, 722, 1595, 1868
Mox20I TGGCCA 2 cut(s) 1296, 1327
Mph1103I ATGCAT 1 cut(s) 1153
MroXI GAANNNNTTC 1 cut(s) 1774
MscI TGGCCA 2 cut(s) 1296, 1327
MseI TTAA 5 cut(s) 518, 792, 915, 1259, 1584
MslI CAYNNNNRTG 3 cut(s) 171, 429, 1425
Msp20I TGGCCA 2 cut(s) 1296, 1327
MspA1I CMGCKG 2 cut(s) 271, 1201
MspI CCGG 3 cut(s) 89, 137, 810
MspR9I CCNGG 4 cut(s) 137, 810, 811, 867
Mva1269I GAATGC 3 cut(s) 285, 1263, 1493
MvaI CCWGG 1 cut(s) 867
MwoI GCNNNNNNNGC 9 cut(s) 118, 127, 277, 1136, 1157, 1269, 1490, 1691, 1799
NciI CCSGG 3 cut(s) 137, 810, 811
NdeII GATC 4 cut(s) 885, 1011, 1231, 1870
NlaIII CATG 8 cut(s) 53, 307, 359, 862, 1073, 1441, 1539, 1637
NlaIV GGNNCC 3 cut(s) 112, 113, 1515
NsiI ATGCAT 1 cut(s) 1153
NspI RCATGY 1 cut(s) 307
NspV TTCGAA 1 cut(s) 289
OliI CACNNNNGTG 2 cut(s) 429, 1425
PagI TCATGA 3 cut(s) 858, 1069, 1437
PceI AGGCCT 1 cut(s) 1644
PcsI WCGNNNNNNNCGW 3 cut(s) 436, 1613, 1771
PctI GAATGC 3 cut(s) 285, 1263, 1493
PdmI GAANNNNTTC 1 cut(s) 1774
PfeI GAWTC 5 cut(s) 180, 862, 1073, 1396, 1459
PkrI GCNGC 5 cut(s) 273, 276, 279, 1203, 1492
PleI GAGTC 5 cut(s) 243, 1072, 1121, 1474, 1893
PpsI GAGTC 5 cut(s) 243, 1072, 1121, 1474, 1893
Ppu21I YACGTR 1 cut(s) 1406
Psp6I CCWGG 1 cut(s) 865
PspGI CCWGG 1 cut(s) 865
PspN4I GGNNCC 3 cut(s) 112, 113, 1515
PspOMI GGGCCC 1 cut(s) 110
PspPI GGNCC 4 cut(s) 110, 111, 755, 1717
PstI CTGCAG 1 cut(s) 1246
PstNI CAGNNNCTG 2 cut(s) 274, 925
PsuI RGATCY 1 cut(s) 1870
PvuII CAGCTG 1 cut(s) 271
RsaI GTAC 2 cut(s) 165, 1515
RsaNI GTAC 2 cut(s) 164, 1514
RseI CAYNNNNRTG 3 cut(s) 171, 429, 1425
SaqAI TTAA 5 cut(s) 518, 792, 915, 1259, 1584
SatI GCNGC 5 cut(s) 272, 275, 278, 1202, 1491
Sau3AI GATC 4 cut(s) 885, 1011, 1231, 1870
Sau96I GGNCC 4 cut(s) 110, 111, 755, 1717
SchI GAGTC 5 cut(s) 244, 1072, 1122, 1475, 1894
ScrFI CCNGG 4 cut(s) 137, 810, 811, 867
SduI GDGCHC 5 cut(s) 114, 1274, 1365, 1411, 1437
SfaNI GCATC 4 cut(s) 1147, 1160, 1216, 1672
SfcI CTRYAG 1 cut(s) 1242
SfuI TTCGAA 1 cut(s) 289
SinI GGWCC 1 cut(s) 755
SmaI CCCGGG 1 cut(s) 811
SmiMI CAYNNNNRTG 3 cut(s) 171, 429, 1425
Sse9I AATT 8 cut(s) 386, 419, 448, 605, 820, 1043, 1235, 1589
SseBI AGGCCT 1 cut(s) 1644
SsiI CCGC 2 cut(s) 566, 1199
SspMI CTAG 1 cut(s) 1912
StuI AGGCCT 1 cut(s) 1644
StyD4I CCNGG 4 cut(s) 135, 808, 809, 865
TaaI ACNGT 5 cut(s) 107, 1424, 1711, 1747, 1775
TaiI ACGT 5 cut(s) 233, 617, 658, 1065, 1408
TasI AATT 8 cut(s) 386, 419, 448, 605, 820, 1043, 1235, 1589
TatI WGTACW 1 cut(s) 163
TfiI GAWTC 5 cut(s) 180, 862, 1073, 1396, 1459
Tru1I TTAA 5 cut(s) 518, 792, 915, 1259, 1584
Tru9I TTAA 5 cut(s) 518, 792, 915, 1259, 1584
TscAI CASTG 2 cut(s) 1365, 1427
TseI GCWGC 5 cut(s) 271, 274, 277, 1201, 1490
TspGWI ACGGA 1 cut(s) 1487
TspMI CCCGGG 1 cut(s) 809
TspRI CASTG 2 cut(s) 1365, 1427
VneI GTGCAC 1 cut(s) 1361
VpaK11BI GGWCC 1 cut(s) 755
XapI RAATTY 4 cut(s) 386, 605, 1043, 1589
XceI RCATGY 1 cut(s) 307
XcmI CCANNNNNNNNNTGG 1 cut(s) 1339
XmaI CCCGGG 1 cut(s) 809
XmnI GAANNNNTTC 1 cut(s) 1774
XspI CTAG 1 cut(s) 1912
Zsp2I ATGCAT 1 cut(s) 1153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.