Prupe.1G167200_v2.0.a1

Rapid ALkalinization Factor (RALF)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
N/A
Physical Location & Seq
Reverse (-)
13595895 .. 13596263
369 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G167200.1_v2.0.a1.CDS.1

Sequence Viewer

Length: 369 bp
TTTGACAAACAAATGAAGATGAAGAGTAGGGTTAGAAGAACCTTTCCCCTATTCTTCATGCTTGCAGCACTTCTAGTGCCACTATTTCTTCAAGCTCTGGCCTCTCATTCAAGAGCCAGTGCTACAGAAACCGGCACCCATGGATATAAAGAACATTGCAGTGGTGAAAATGGTGAAGAGTTTAAAATGGAGTACGAAACAAGTAGGAGGTTTCTTGCAGTTCAAAAATTTCTGTCTCCAGGAGTTCTCAAGAGGGATCTGCCAGTTTGTAAGGGTCCACGAGGAGAACCCTACGGCATCAGTTGTCTCCCTCCACCATCAAACCCTAGGTATAGAGCCTGCTCCAAAATCTACAGGTGCAGAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RALF

Protein Analysis

123

Amino Acids

13.89

Weight (kDa)

9.82

Isoelectric Point (pI)

50.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 134
AclWI GGATC 1 cut(s) 264
AcsI RAATTY 1 cut(s) 227
AfaI GTAC 1 cut(s) 194
AgsI TTSAA 3 cut(s) 92, 111, 224
AjnI CCWGG 1 cut(s) 238
AluBI AGCT 2 cut(s) 95, 365
AluI AGCT 2 cut(s) 95, 365
Alw26I GTCTC 2 cut(s) 240, 311
AlwI GGATC 1 cut(s) 264
AoxI GGCC 1 cut(s) 99
ApeKI GCWGC 1 cut(s) 65
ApoI RAATTY 1 cut(s) 227
AspA2I CCTAGG 1 cut(s) 326
AspS9I GGNCC 1 cut(s) 275
AsuHPI GGTGA 2 cut(s) 176, 185
AvaII GGWCC 1 cut(s) 275
AvrII CCTAGG 1 cut(s) 326
BanI GGYRCC 1 cut(s) 134
BauI CACGAG 1 cut(s) 279
BbvI GCAGC 1 cut(s) 77
BccI CCATC 1 cut(s) 325
BceAI ACGGC 1 cut(s) 310
BciT130I CCWGG 1 cut(s) 240
BcoDI GTCTC 2 cut(s) 240, 311
BfaI CTAG 2 cut(s) 74, 327
BfmI CTRYAG 2 cut(s) 123, 352
BisI GCNGC 1 cut(s) 66
BlnI CCTAGG 1 cut(s) 326
BlsI GCNGC 1 cut(s) 67
Bme1390I CCNGG 1 cut(s) 240
Bme18I GGWCC 1 cut(s) 275
BmgT120I GGNCC 1 cut(s) 275
BmiI GGNNCC 2 cut(s) 136, 276
BmrFI CCNGG 1 cut(s) 240
BmsI GCATC 1 cut(s) 306
BpmI CTGGAG 1 cut(s) 222
BpuEI CTTGAG 1 cut(s) 233
BsaJI CCNNGG 2 cut(s) 139, 326
Bse118I RCCGGY 1 cut(s) 131
Bse1I ACTGG 2 cut(s) 117, 263
Bse3DI GCAATG 1 cut(s) 154
BseBI CCWGG 1 cut(s) 240
BseDI CCNNGG 2 cut(s) 139, 326
BseMI GCAATG 1 cut(s) 154
BseNI ACTGG 2 cut(s) 117, 263
BseRI GAGGAG 1 cut(s) 297
BseXI GCAGC 1 cut(s) 77
BshFI GGCC 1 cut(s) 101
BshNI GGYRCC 1 cut(s) 134
BsiSI CCGG 1 cut(s) 132
BsmAI GTCTC 2 cut(s) 240, 311
BsnI GGCC 1 cut(s) 101
Bsp143I GATC 1 cut(s) 256
Bsp19I CCATGG 1 cut(s) 139
BspANI GGCC 1 cut(s) 101
BspLI GGNNCC 2 cut(s) 136, 276
BspPI GGATC 1 cut(s) 264
BspT107I GGYRCC 1 cut(s) 134
BsrDI GCAATG 1 cut(s) 154
BsrFI RCCGGY 1 cut(s) 131
BsrI ACTGG 2 cut(s) 117, 263
BssAI RCCGGY 1 cut(s) 131
BssECI CCNNGG 2 cut(s) 139, 326
BssMI GATC 1 cut(s) 256
BssSI CACGAG 1 cut(s) 279
BssT1I CCWWGG 2 cut(s) 139, 326
Bst2BI CACGAG 1 cut(s) 279
Bst2UI CCWGG 1 cut(s) 240
Bst6I CTCTTC 2 cut(s) 17, 171
BstC8I GCNNGC 2 cut(s) 63, 340
BstDSI CCRYGG 1 cut(s) 139
BstKTI GATC 1 cut(s) 259
BstMAI GTCTC 2 cut(s) 240, 311
BstMBI GATC 1 cut(s) 256
BstNI CCWGG 1 cut(s) 240
BstSCI CCNGG 1 cut(s) 238
BstSFI CTRYAG 2 cut(s) 123, 352
BstV1I GCAGC 1 cut(s) 77
BstX2I RGATCY 1 cut(s) 256
BstYI RGATCY 1 cut(s) 256
BsuRI GGCC 1 cut(s) 101
BtgI CCRYGG 1 cut(s) 139
BtsI GCAGTG 1 cut(s) 166
BtsIMutI CAGTG 2 cut(s) 124, 166
Cac8I GCNNGC 2 cut(s) 63, 340
Cfr10I RCCGGY 1 cut(s) 131
Cfr13I GGNCC 1 cut(s) 275
Csp6I GTAC 1 cut(s) 193
CviAII CATG 2 cut(s) 58, 140
CviJI RGCY 5 cut(s) 95, 101, 116, 338, 365
CviKI_1 RGCY 5 cut(s) 95, 101, 116, 338, 365
CviQI GTAC 1 cut(s) 193
DpnI GATC 1 cut(s) 258
DpnII GATC 1 cut(s) 256
DraI TTTAAA 1 cut(s) 184
Eam1104I CTCTTC 2 cut(s) 17, 171
EarI CTCTTC 2 cut(s) 17, 171
Eco130I CCWWGG 2 cut(s) 139, 326
Eco47I GGWCC 1 cut(s) 275
EcoRII CCWGG 1 cut(s) 238
EcoT14I CCWWGG 2 cut(s) 139, 326
ErhI CCWWGG 2 cut(s) 139, 326
FaeI CATG 2 cut(s) 61, 143
FaiI YATR 4 cut(s) 59, 141, 147, 333
FatI CATG 2 cut(s) 57, 139
Fnu4HI GCNGC 1 cut(s) 66
Fsp4HI GCNGC 1 cut(s) 66
FspBI CTAG 2 cut(s) 74, 327
GluI GCNGC 1 cut(s) 66
GsuI CTGGAG 1 cut(s) 222
HaeIII GGCC 1 cut(s) 101
HapII CCGG 1 cut(s) 132
Hin1II CATG 2 cut(s) 61, 143
HpaII CCGG 1 cut(s) 132
HphI GGTGA 2 cut(s) 176, 185
Hpy166II GTNNAC 1 cut(s) 278
Hpy188III TCNNGA 2 cut(s) 111, 250
Hpy8I GTNNAC 1 cut(s) 278
HpyCH4V TGCA 4 cut(s) 65, 159, 218, 360
Hsp92II CATG 2 cut(s) 61, 143
Kzo9I GATC 1 cut(s) 256
LmnI GCTCC 1 cut(s) 347
LpnPI CCDG 8 cut(s) 83, 130, 145, 225, 252, 276, 340, 352
Lsp1109I GCAGC 1 cut(s) 77
LweI GCATC 1 cut(s) 306
MaeI CTAG 2 cut(s) 74, 327
MalI GATC 1 cut(s) 258
MboI GATC 1 cut(s) 256
MboII GAAGA 6 cut(s) 28, 34, 46, 48, 80, 188
MflI RGATCY 1 cut(s) 256
MluCI AATT 1 cut(s) 227
MnlI CCTC 5 cut(s) 112, 201, 246, 275, 321
MseI TTAA 1 cut(s) 183
MslI CAYNNNNRTG 1 cut(s) 159
MspI CCGG 1 cut(s) 132
MspR9I CCNGG 1 cut(s) 240
MvaI CCWGG 1 cut(s) 240
NcoI CCATGG 1 cut(s) 139
NdeII GATC 1 cut(s) 256
NlaIII CATG 2 cut(s) 61, 143
NlaIV GGNNCC 2 cut(s) 136, 276
PfoI TCCNGGA 1 cut(s) 238
PkrI GCNGC 1 cut(s) 67
Psp6I CCWGG 1 cut(s) 238
PspGI CCWGG 1 cut(s) 238
PspN4I GGNNCC 2 cut(s) 136, 276
PspPI GGNCC 1 cut(s) 275
PsuI RGATCY 1 cut(s) 256
RsaI GTAC 1 cut(s) 194
RsaNI GTAC 1 cut(s) 193
RseI CAYNNNNRTG 1 cut(s) 159
SaqAI TTAA 1 cut(s) 183
SatI GCNGC 1 cut(s) 66
Sau3AI GATC 1 cut(s) 256
Sau96I GGNCC 1 cut(s) 275
ScrFI CCNGG 1 cut(s) 240
SetI ASST 6 cut(s) 44, 97, 212, 332, 359, 367
SfaNI GCATC 1 cut(s) 306
SfcI CTRYAG 2 cut(s) 123, 352
SinI GGWCC 1 cut(s) 275
SmiMI CAYNNNNRTG 1 cut(s) 159
SmlI CTYRAG 1 cut(s) 248
SmoI CTYRAG 1 cut(s) 248
Sse9I AATT 1 cut(s) 227
SspMI CTAG 2 cut(s) 74, 327
StyD4I CCNGG 1 cut(s) 238
StyI CCWWGG 2 cut(s) 139, 326
TasI AATT 1 cut(s) 227
Tru1I TTAA 1 cut(s) 183
Tru9I TTAA 1 cut(s) 183
TscAI CASTG 2 cut(s) 124, 166
TseI GCWGC 1 cut(s) 65
TspDTI ATGAA 3 cut(s) 29, 35, 46
TspRI CASTG 2 cut(s) 124, 166
VpaK11BI GGWCC 1 cut(s) 275
XapI RAATTY 1 cut(s) 227
XmaJI CCTAGG 1 cut(s) 326
XspI CTAG 2 cut(s) 74, 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.