Prupe.1G173500_v2.0.a1

Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
N/A
Physical Location & Seq
Reverse (-)
14554516 .. 14559917
5402 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G173500.1_v2.0.a1.CDS.5

Sequence Viewer

Length: 765 bp
ATGTCTACAAGAAAGGCTAGTACGGATGTCCATGAATTTGAGGGAATGTTTCTAGGGTTTCATGTGCATTTTAAAGTGACATCGGTTATTGGGCATGTTTTCAGGTTAATTTTTCTCCCGAAGCTATCACCTTTGAAATCTAGCAATCCTTCCCAAATGTTAAATTGTCAATGCTATCTGCTACGGGCCATGACAGACTCCCACCCTTGGTTTTGCGTACAACGTTATCTGCAGATAAATACATTCAAACCGGAAAAGTTTTGGGCTGTACGCCCTTACATAATACAAAAGGGCTATGAACTTCAGCTAGAATGGGAACGTAACAAGTTGTTTGACTACGATGTTGCTGTAATGTTTCAAAAGTTAGTAATGGAAGATGGAATTCTAGAAGTGACTGAGATGGTAGAAAAGCAAGAAAGCAAAAGTCGTCCTTTTGGTCTTAATACAGTCAATCTTCTGAAGGTTGCTTCAAGTGCACTCAGCTATCCGCGTACAGAGAGCACAGCTTATCCTTCTTCTTTTGACTTTAGAGGCACACTTCGTGCGCAAGTAAATAACCCAACATGGGCCAGTTATGTTCAGAGGCTGCTTGCTGATGGTTATCAAAAGCCACGATCCGGGACAGATGTAGGTGACCATCCTCCTGTTACTCCATTGAGGTCTGCAACTGAGGACATGCTAGGAAATGATGCTGGGAGACTATATGAGTACATTTGTACACACTTCATAGGGACTGTATCTCCTGACTGCAAATATGTAAGGTAA

Protein Analysis

255

Amino Acids

29.23

Weight (kDa)

8.51

Isoelectric Point (pI)

31.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 546
AccI GTMKAC 1 cut(s) 5
AccII CGCG 1 cut(s) 490
AciI CCGC 1 cut(s) 488
AclI AACGTT 1 cut(s) 223
AclWI GGATC 1 cut(s) 609
AcsI RAATTY 2 cut(s) 35, 381
AcuI CTGAAG 2 cut(s) 287, 479
AdeI CACNNNGTG 1 cut(s) 542
AfaI GTAC 6 cut(s) 22, 219, 270, 493, 710, 718
AfiI CCNNNNNNNGG 3 cut(s) 207, 565, 617
AgsI TTSAA 4 cut(s) 136, 247, 359, 471
AluBI AGCT 4 cut(s) 124, 307, 483, 506
AluI AGCT 4 cut(s) 124, 307, 483, 506
Alw21I GWGCWC 2 cut(s) 478, 503
Alw26I GTCTC 1 cut(s) 691
Alw44I GTGCAC 1 cut(s) 474
AlwI GGATC 1 cut(s) 609
AlwNI CAGNNNCTG 1 cut(s) 586
AoxI GGCC 2 cut(s) 186, 567
ApaLI GTGCAC 1 cut(s) 474
ApeKI GCWGC 1 cut(s) 586
ApoI RAATTY 2 cut(s) 35, 381
AspLEI GCGC 1 cut(s) 547
AspS9I GGNCC 2 cut(s) 186, 567
AsuC2I CCSGG 1 cut(s) 619
AsuHPI GGTGA 2 cut(s) 120, 644
BaeGI GKGCMC 1 cut(s) 478
Bbv12I GWGCWC 2 cut(s) 478, 503
BbvI GCAGC 1 cut(s) 573
BccI CCATC 4 cut(s) 371, 394, 590, 645
BcnI CCSGG 1 cut(s) 619
BcoDI GTCTC 1 cut(s) 691
BfaI CTAG 6 cut(s) 18, 53, 141, 308, 386, 680
BfmI CTRYAG 1 cut(s) 230
BisI GCNGC 1 cut(s) 587
BlsI GCNGC 1 cut(s) 588
Bme1390I CCNGG 1 cut(s) 619
BmgT120I GGNCC 2 cut(s) 186, 567
BmrFI CCNGG 1 cut(s) 619
BmsI GCATC 1 cut(s) 679
BpuMI CCSGG 1 cut(s) 619
BsaBI GATNNNNATC 1 cut(s) 600
BsaJI CCNNGG 1 cut(s) 206
BsaWI WCCGGW 1 cut(s) 250
BsaXI ACNNNNNCTCC 2 cut(s) 724, 754
Bsc4I CCNNNNNNNGG 3 cut(s) 207, 565, 617
Bse1I ACTGG 1 cut(s) 570
Bse8I GATNNNNATC 1 cut(s) 600
BseDI CCNNGG 1 cut(s) 206
BseGI GGATG 2 cut(s) 31, 637
BseJI GATNNNNATC 1 cut(s) 600
BseLI CCNNNNNNNGG 3 cut(s) 207, 565, 617
BseMII CTCAG 3 cut(s) 387, 493, 660
BseNI ACTGG 1 cut(s) 570
BseSI GKGCMC 1 cut(s) 478
BseXI GCAGC 1 cut(s) 573
BseYI CCCAGC 1 cut(s) 692
Bsh1236I CGCG 1 cut(s) 490
BshFI GGCC 2 cut(s) 188, 569
BsiHKAI GWGCWC 2 cut(s) 478, 503
BsiSI CCGG 2 cut(s) 251, 618
BslFI GGGAC 2 cut(s) 634, 745
BslI CCNNNNNNNGG 3 cut(s) 207, 565, 617
BsmAI GTCTC 1 cut(s) 691
BsmFI GGGAC 2 cut(s) 634, 745
BsnI GGCC 2 cut(s) 188, 569
Bsp1286I GDGCHC 2 cut(s) 478, 503
Bsp1407I TGTACA 1 cut(s) 716
Bsp143I GATC 1 cut(s) 614
BspACI CCGC 1 cut(s) 488
BspANI GGCC 2 cut(s) 188, 569
BspCNI CTCAG 3 cut(s) 388, 492, 661
BspFNI CGCG 1 cut(s) 490
BspMAI CTGCAG 1 cut(s) 234
BspPI GGATC 1 cut(s) 609
BsrGI TGTACA 1 cut(s) 716
BsrI ACTGG 1 cut(s) 570
BssECI CCNNGG 1 cut(s) 206
BssMI GATC 1 cut(s) 614
BssT1I CCWWGG 1 cut(s) 206
Bst4CI ACNGT 2 cut(s) 448, 736
BstAUI TGTACA 1 cut(s) 716
BstC8I GCNNGC 1 cut(s) 591
BstDEI CTNAG 3 cut(s) 396, 479, 669
BstEII GGTNACC 1 cut(s) 632
BstF5I GGATG 2 cut(s) 31, 637
BstFNI CGCG 1 cut(s) 490
BstHHI GCGC 1 cut(s) 547
BstKTI GATC 1 cut(s) 617
BstMAI GTCTC 1 cut(s) 691
BstMBI GATC 1 cut(s) 614
BstMWI GCNNNNNNNGC 1 cut(s) 473
BstNSI RCATGY 2 cut(s) 98, 679
BstPI GGTNACC 1 cut(s) 632
BstSCI CCNGG 1 cut(s) 617
BstSFI CTRYAG 1 cut(s) 230
BstSLI GKGCMC 1 cut(s) 478
BstUI CGCG 1 cut(s) 490
BstV1I GCAGC 1 cut(s) 573
BsuRI GGCC 2 cut(s) 188, 569
BtsCI GGATG 2 cut(s) 31, 637
Cac8I GCNNGC 1 cut(s) 591
CaiI CAGNNNCTG 1 cut(s) 586
CfoI GCGC 1 cut(s) 547
Cfr13I GGNCC 2 cut(s) 186, 567
Csp6I GTAC 6 cut(s) 21, 218, 269, 492, 709, 717
CviAII CATG 6 cut(s) 32, 62, 95, 190, 564, 676
CviQI GTAC 6 cut(s) 21, 218, 269, 492, 709, 717
DdeI CTNAG 3 cut(s) 396, 479, 669
DpnI GATC 1 cut(s) 616
DpnII GATC 1 cut(s) 614
DraI TTTAAA 1 cut(s) 73
DraIII CACNNNGTG 1 cut(s) 542
Eco130I CCWWGG 1 cut(s) 206
Eco57I CTGAAG 2 cut(s) 287, 479
Eco91I GGTNACC 1 cut(s) 632
EcoO65I GGTNACC 1 cut(s) 632
EcoRI GAATTC 1 cut(s) 381
EcoT14I CCWWGG 1 cut(s) 206
ErhI CCWWGG 1 cut(s) 206
FaeI CATG 6 cut(s) 35, 65, 98, 193, 567, 679
FalI AAGNNNNNCTT 2 cut(s) 415, 447
FaqI GGGAC 2 cut(s) 634, 745
FatI CATG 6 cut(s) 31, 61, 94, 189, 563, 675
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 1 cut(s) 587
FokI GGATG 2 cut(s) 38, 624
Fsp4HI GCNGC 1 cut(s) 587
FspBI CTAG 6 cut(s) 18, 53, 141, 308, 386, 680
FspI TGCGCA 1 cut(s) 546
GlaI GCGC 1 cut(s) 546
GluI GCNGC 1 cut(s) 587
GsaI CCCAGC 1 cut(s) 696
HaeIII GGCC 2 cut(s) 188, 569
HapII CCGG 2 cut(s) 251, 618
HhaI GCGC 1 cut(s) 547
Hin1II CATG 6 cut(s) 35, 65, 98, 193, 567, 679
Hin6I GCGC 1 cut(s) 545
HinP1I GCGC 1 cut(s) 545
HinfI GANTC 1 cut(s) 197
HpaII CCGG 2 cut(s) 251, 618
HphI GGTGA 2 cut(s) 120, 644
Hpy166II GTNNAC 3 cut(s) 6, 476, 719
Hpy188I TCNGA 2 cut(s) 459, 582
Hpy188III TCNNGA 3 cut(s) 118, 386, 743
Hpy8I GTNNAC 3 cut(s) 6, 476, 719
HpyAV CCTTC 3 cut(s) 159, 454, 522
HpyCH4III ACNGT 2 cut(s) 448, 736
HpyCH4IV ACGT 2 cut(s) 223, 319
HpyCH4V TGCA 5 cut(s) 67, 232, 476, 665, 750
HpyF10VI GCNNNNNNNGC 1 cut(s) 473
HpyF3I CTNAG 3 cut(s) 396, 479, 669
HpySE526I ACGT 2 cut(s) 223, 319
Hsp92II CATG 6 cut(s) 35, 65, 98, 193, 567, 679
HspAI GCGC 1 cut(s) 545
Kzo9I GATC 1 cut(s) 614
LpnPI CCDG 7 cut(s) 88, 264, 583, 631, 657, 678, 756
Lsp1109I GCAGC 1 cut(s) 573
LweI GCATC 1 cut(s) 679
MaeI CTAG 6 cut(s) 18, 53, 141, 308, 386, 680
MaeII ACGT 2 cut(s) 223, 319
MaeIII GTNAC 5 cut(s) 76, 320, 391, 632, 646
MalI GATC 1 cut(s) 616
MboI GATC 1 cut(s) 614
MboII GAAGA 3 cut(s) 386, 446, 507
MhlI GDGCHC 2 cut(s) 478, 503
MluCI AATT 4 cut(s) 35, 108, 163, 381
MlyI GAGTC 1 cut(s) 191
MnlI CCTC 6 cut(s) 34, 524, 576, 651, 651, 664
MseI TTAA 4 cut(s) 72, 107, 161, 441
MspI CCGG 2 cut(s) 251, 618
MspR9I CCNGG 1 cut(s) 619
MvnI CGCG 1 cut(s) 490
MwoI GCNNNNNNNGC 1 cut(s) 473
NciI CCSGG 1 cut(s) 619
NdeII GATC 1 cut(s) 614
NlaIII CATG 6 cut(s) 35, 65, 98, 193, 567, 679
NmuCI GTSAC 3 cut(s) 76, 391, 632
NsbI TGCGCA 1 cut(s) 546
NspI RCATGY 2 cut(s) 98, 679
PfoI TCCNGGA 1 cut(s) 617
PkrI GCNGC 1 cut(s) 588
PleI GAGTC 1 cut(s) 191
PpsI GAGTC 1 cut(s) 191
Psp1406I AACGTT 1 cut(s) 223
PspEI GGTNACC 1 cut(s) 632
PspFI CCCAGC 1 cut(s) 692
PspPI GGNCC 2 cut(s) 186, 567
PstI CTGCAG 1 cut(s) 234
PstNI CAGNNNCTG 1 cut(s) 586
RsaI GTAC 6 cut(s) 22, 219, 270, 493, 710, 718
RsaNI GTAC 6 cut(s) 21, 218, 269, 492, 709, 717
SaqAI TTAA 4 cut(s) 72, 107, 161, 441
SatI GCNGC 1 cut(s) 587
Sau3AI GATC 1 cut(s) 614
Sau96I GGNCC 2 cut(s) 186, 567
SchI GAGTC 1 cut(s) 191
ScrFI CCNGG 1 cut(s) 619
SduI GDGCHC 2 cut(s) 478, 503
SfaNI GCATC 1 cut(s) 679
SfcI CTRYAG 1 cut(s) 230
Sse9I AATT 4 cut(s) 35, 108, 163, 381
SsiI CCGC 1 cut(s) 488
SspMI CTAG 6 cut(s) 18, 53, 141, 308, 386, 680
StyD4I CCNGG 1 cut(s) 617
StyI CCWWGG 1 cut(s) 206
TaaI ACNGT 2 cut(s) 448, 736
TaiI ACGT 2 cut(s) 226, 322
TasI AATT 4 cut(s) 35, 108, 163, 381
TatI WGTACW 2 cut(s) 708, 716
Tru1I TTAA 4 cut(s) 72, 107, 161, 441
Tru9I TTAA 4 cut(s) 72, 107, 161, 441
TseFI GTSAC 3 cut(s) 76, 391, 632
TseI GCWGC 1 cut(s) 586
Tsp45I GTSAC 3 cut(s) 76, 391, 632
TspDTI ATGAA 4 cut(s) 48, 50, 312, 715
TspGWI ACGGA 1 cut(s) 38
VneI GTGCAC 1 cut(s) 474
XapI RAATTY 2 cut(s) 35, 381
XbaI TCTAGA 1 cut(s) 385
XceI RCATGY 2 cut(s) 98, 679
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 6 cut(s) 18, 53, 141, 308, 386, 680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.