Prupe.1G318900_v2.0.a1

Serine threonine-protein kinase At1g18390

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
N/A
Physical Location & Seq
Reverse (-)
30631259 .. 30632968
1710 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G318900.1_v2.0.a1.CDS.4

Sequence Viewer

Length: 1227 bp
ATGACAAGGAAAGAACTATTTATTGCTTCAGTTATTCTCTCTTCTGTGGTGATCTATCAATCCATCAATGAAGTTGAAGCCAGAATTCTTGAGCAAGTTTGTCCTTCTTCTTCATGTGGAGATATCCAAAACATCAGCTTCCCTTTTCACCTAAAGGGTGATCCAGTAGGCTGCGGCTATCCGGCCTATGAACTTTCTTGTGTGAACAACAAAACCATCTTGGAATACGATCCAGGAACATACTATGTCAAGAACATTTCCTATAATGAACATTCAGTGGATGAGTTAACCGACGATTTTCCTTATAGGCCACTAGGGCATCTCTCATATATCAGTTTTGCAAACTGCTCCACAAACATAAGCAACCTAGCTTCTATAGAATTTCCTTGTTTCAGAAGAAATGGCACTCATCAAGAAAAATCCTGTTCTCTCATTTCAATTGCTCCTGCAGATTCTGTAGATGTCAAGTTCCCTTCTTATGAAGATGTCATGAAAGTGTTGCAGGCAGGGTTCAATCTTGGATGGGAGTATTATGTATGGTTCGTATATGTGAGCTTCGACTTATTCTGGAACATTCCATTAGCCCTAATTCTTATTATTAGACATATCTGTGCTCCTTTGGCTATATTTGTGTTCCTCATCCACAAATATTGTACAACACGAAAGAGAGTAGATAACAGGGAGATAGTTGTGCTGCACAATGAGCAATCCTTGATGCCCACAAGGTACTCTTACACGGATGTAGTTGCAATGACAAACAACTTTGAAGATAAACTAGGCCTGCAGGGTGGGCTTGGGTCAGTATATAAAGGGCAGCTTCCAGATGGTTGCCTAATTGTTGTCAAAATGCTGGAGAACTCTAGATTTCAGTCATTTAAGTGGGAAAAGCTGCATGAGTTTGCTCTGCGAATCGCTCGAGGGATCGAGTATCTACACAAGGGAAGTGATGTGTGCATTCTTCATTTAGAAATGAAGCCTCAAAATGTCCTGCTAGACCACAATTTCATCCCCAAAATATCAGATGCTGGCCTTGCAAAATTTTACCCGGAGGACTATGATTTTGTTTTTGTTAGTACTTTAAGAGGAACAGCAGGGTACATAGCGCCTGAACTGAATTCTAGGAATGTTGGAACTGTTTCTAGCAAATCAGATGTTTATAGCTTTGGGATGTTGATGTTGGGAATGGCAGGAGGAAGAATGGATGTCAACCCAAAGGCAAGCAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind WAK_assoc

Protein Analysis

409

Amino Acids

46.1

Weight (kDa)

5.9

Isoelectric Point (pI)

46.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 174
AclWI GGATC 3 cut(s) 155, 224, 929
AcsI RAATTY 4 cut(s) 84, 380, 1037, 1114
AcuI CTGAAG 1 cut(s) 12
AfaI GTAC 4 cut(s) 655, 728, 1075, 1097
AgsI TTSAA 4 cut(s) 77, 438, 514, 767
AjnI CCWGG 1 cut(s) 232
AluBI AGCT 7 cut(s) 138, 371, 555, 817, 889, 1161, 1224
AluI AGCT 7 cut(s) 138, 371, 555, 817, 889, 1161, 1224
Alw21I GWGCWC 1 cut(s) 616
AlwI GGATC 3 cut(s) 155, 224, 929
AlwNI CAGNNNCTG 2 cut(s) 455, 1025
Ama87I CYCGRG 1 cut(s) 915
AoxI GGCC 4 cut(s) 183, 308, 778, 1027
ApeKI GCWGC 5 cut(s) 171, 694, 814, 889, 1221
ApoI RAATTY 4 cut(s) 84, 380, 1037, 1114
ArsI GACNNNNNNTTYG 2 cut(s) 1043, 1075
Asp700I GAANNNNTTC 1 cut(s) 1135
AspLEI GCGC 1 cut(s) 1105
AsuC2I CCSGG 1 cut(s) 1046
AsuHPI GGTGA 3 cut(s) 61, 140, 170
AvaI CYCGRG 1 cut(s) 915
Bbv12I GWGCWC 1 cut(s) 616
BbvI GCAGC 4 cut(s) 158, 681, 826, 876
BccI CCATC 4 cut(s) 71, 224, 516, 818
BciT130I CCWGG 1 cut(s) 234
BcnI CCSGG 1 cut(s) 1046
BfaI CTAG 7 cut(s) 314, 368, 776, 861, 992, 1119, 1140
BfmI CTRYAG 4 cut(s) 375, 447, 456, 782
BfoI RGCGCY 1 cut(s) 1106
BglI GCCNNNNNGGC 1 cut(s) 316
BisI GCNGC 6 cut(s) 172, 175, 695, 815, 890, 1222
BlsI GCNGC 6 cut(s) 173, 176, 696, 816, 891, 1223
BmcAI AGTACT 1 cut(s) 1075
Bme1390I CCNGG 2 cut(s) 234, 1046
BmeT110I CYCGRG 1 cut(s) 915
BmrFI CCNGG 2 cut(s) 234, 1046
BmsI GCATC 3 cut(s) 328, 705, 1012
BpmI CTGGAG 1 cut(s) 872
BpuEI CTTGAG 1 cut(s) 110
BpuMI CCSGG 1 cut(s) 1046
Bse1I ACTGG 1 cut(s) 164
Bse3DI GCAATG 1 cut(s) 756
BseBI CCWGG 1 cut(s) 234
BseGI GGATG 7 cut(s) 286, 527, 639, 745, 1005, 1173, 1207
BseMI GCAATG 1 cut(s) 756
BseNI ACTGG 1 cut(s) 164
BseXI GCAGC 4 cut(s) 158, 681, 826, 876
BsgI GTGCAG 1 cut(s) 680
BshFI GGCC 4 cut(s) 185, 310, 780, 1029
BsiHKAI GWGCWC 1 cut(s) 616
BsiHKCI CYCGRG 1 cut(s) 915
BsiSI CCGG 2 cut(s) 182, 1046
BsmI GAATGC 1 cut(s) 954
BsnI GGCC 4 cut(s) 185, 310, 780, 1029
BsoBI CYCGRG 1 cut(s) 915
Bsp1286I GDGCHC 1 cut(s) 616
Bsp1407I TGTACA 1 cut(s) 653
Bsp143I GATC 4 cut(s) 51, 160, 229, 921
BspACI CCGC 1 cut(s) 174
BspANI GGCC 4 cut(s) 185, 310, 780, 1029
BspHI TCATGA 1 cut(s) 489
BspMAI CTGCAG 2 cut(s) 451, 786
BspPI GGATC 3 cut(s) 155, 224, 929
BsrDI GCAATG 1 cut(s) 756
BsrGI TGTACA 1 cut(s) 653
BsrI ACTGG 1 cut(s) 164
BssMI GATC 4 cut(s) 51, 160, 229, 921
Bst2UI CCWGG 1 cut(s) 234
Bst4CI ACNGT 1 cut(s) 1135
Bst6I CTCTTC 1 cut(s) 46
BstAUI TGTACA 1 cut(s) 653
BstC8I GCNNGC 4 cut(s) 504, 782, 1027, 1219
BstF5I GGATG 7 cut(s) 286, 527, 639, 745, 1005, 1173, 1207
BstH2I RGCGCY 1 cut(s) 1106
BstHHI GCGC 1 cut(s) 1105
BstKTI GATC 4 cut(s) 54, 163, 232, 924
BstMBI GATC 4 cut(s) 51, 160, 229, 921
BstMWI GCNNNNNNNGC 5 cut(s) 316, 620, 703, 790, 1031
BstNI CCWGG 1 cut(s) 234
BstSCI CCNGG 2 cut(s) 232, 1044
BstSFI CTRYAG 4 cut(s) 375, 447, 456, 782
BstV1I GCAGC 4 cut(s) 158, 681, 826, 876
BsuRI GGCC 4 cut(s) 185, 310, 780, 1029
BtsCI GGATG 7 cut(s) 286, 527, 639, 745, 1005, 1173, 1207
BtsIMutI CAGTG 1 cut(s) 282
Cac8I GCNNGC 4 cut(s) 504, 782, 1027, 1219
CaiI CAGNNNCTG 2 cut(s) 455, 1025
CciI TCATGA 1 cut(s) 489
CfoI GCGC 1 cut(s) 1105
Csp6I GTAC 4 cut(s) 654, 727, 1074, 1096
CspCI CAANNNNNGTGG 2 cut(s) 632, 667
CviAII CATG 3 cut(s) 114, 490, 893
CviQI GTAC 4 cut(s) 654, 727, 1074, 1096
DpnI GATC 4 cut(s) 53, 162, 231, 923
DpnII GATC 4 cut(s) 51, 160, 229, 921
Eam1104I CTCTTC 1 cut(s) 46
EarI CTCTTC 1 cut(s) 46
Eco147I AGGCCT 1 cut(s) 780
Eco32I GATATC 1 cut(s) 124
Eco57I CTGAAG 1 cut(s) 12
Eco88I CYCGRG 1 cut(s) 915
EcoRI GAATTC 2 cut(s) 84, 1114
EcoRII CCWGG 1 cut(s) 232
EcoRV GATATC 1 cut(s) 124
FaeI CATG 3 cut(s) 117, 493, 896
FalI AAGNNNNNCTT 4 cut(s) 715, 747, 801, 833
FatI CATG 3 cut(s) 113, 489, 892
Fnu4HI GCNGC 6 cut(s) 172, 175, 695, 815, 890, 1222
FokI GGATG 7 cut(s) 293, 534, 626, 752, 992, 1180, 1214
Fsp4HI GCNGC 6 cut(s) 172, 175, 695, 815, 890, 1222
FspBI CTAG 7 cut(s) 314, 368, 776, 861, 992, 1119, 1140
GlaI GCGC 1 cut(s) 1104
GluI GCNGC 6 cut(s) 172, 175, 695, 815, 890, 1222
GsuI CTGGAG 1 cut(s) 872
HaeII RGCGCY 1 cut(s) 1106
HaeIII GGCC 4 cut(s) 185, 310, 780, 1029
HapII CCGG 2 cut(s) 182, 1046
HhaI GCGC 1 cut(s) 1105
Hin1II CATG 3 cut(s) 117, 493, 896
Hin6I GCGC 1 cut(s) 1103
HinP1I GCGC 1 cut(s) 1103
HincII GTYRAC 2 cut(s) 288, 1207
HindII GTYRAC 2 cut(s) 288, 1207
HinfI GANTC 2 cut(s) 452, 909
HpaI GTTAAC 1 cut(s) 288
HpaII CCGG 2 cut(s) 182, 1046
HphI GGTGA 3 cut(s) 61, 140, 170
Hpy166II GTNNAC 3 cut(s) 205, 288, 1207
Hpy188I TCNGA 3 cut(s) 395, 1021, 1150
Hpy188III TCNNGA 7 cut(s) 89, 250, 413, 490, 568, 821, 861
Hpy8I GTNNAC 3 cut(s) 205, 288, 1207
Hpy99I CGWCG 1 cut(s) 296
HpyAV CCTTC 2 cut(s) 114, 483
HpyCH4III ACNGT 1 cut(s) 1135
HpyCH4V TGCA 9 cut(s) 341, 449, 502, 697, 749, 784, 892, 954, 1034
HpyF10VI GCNNNNNNNGC 5 cut(s) 316, 620, 703, 790, 1031
Hsp92II CATG 3 cut(s) 117, 493, 896
HspAI GCGC 1 cut(s) 1103
KspAI GTTAAC 1 cut(s) 288
Kzo9I GATC 4 cut(s) 51, 160, 229, 921
LmnI GCTCC 3 cut(s) 353, 448, 619
Lsp1109I GCAGC 4 cut(s) 158, 681, 826, 876
LweI GCATC 3 cut(s) 328, 705, 1012
MaeI CTAG 7 cut(s) 314, 368, 776, 861, 992, 1119, 1140
MalI GATC 4 cut(s) 53, 162, 231, 923
MboI GATC 4 cut(s) 51, 160, 229, 921
MboII GAAGA 8 cut(s) 33, 99, 102, 408, 494, 779, 950, 1206
MfeI CAATTG 1 cut(s) 438
MhlI GDGCHC 1 cut(s) 616
MluCI AATT 8 cut(s) 84, 380, 438, 588, 834, 1000, 1037, 1114
MmeI TCCRAC 1 cut(s) 1108
MnlI CCTC 6 cut(s) 647, 911, 987, 1042, 1076, 1184
MroXI GAANNNNTTC 1 cut(s) 1135
MseI TTAA 3 cut(s) 287, 876, 1079
MslI CAYNNNNRTG 3 cut(s) 494, 609, 877
MspI CCGG 2 cut(s) 182, 1046
MspR9I CCNGG 2 cut(s) 234, 1046
MunI CAATTG 1 cut(s) 438
Mva1269I GAATGC 1 cut(s) 954
MvaI CCWGG 1 cut(s) 234
MwoI GCNNNNNNNGC 5 cut(s) 316, 620, 703, 790, 1031
NciI CCSGG 1 cut(s) 1046
NdeII GATC 4 cut(s) 51, 160, 229, 921
NlaIII CATG 3 cut(s) 117, 493, 896
PaeR7I CTCGAG 1 cut(s) 915
PagI TCATGA 1 cut(s) 489
PceI AGGCCT 1 cut(s) 780
PctI GAATGC 1 cut(s) 954
PdmI GAANNNNTTC 1 cut(s) 1135
PfeI GAWTC 2 cut(s) 452, 909
PfoI TCCNGGA 1 cut(s) 232
PkrI GCNGC 6 cut(s) 173, 176, 696, 816, 891, 1223
Psp6I CCWGG 1 cut(s) 232
PspGI CCWGG 1 cut(s) 232
PspXI VCTCGAGB 1 cut(s) 915
PstI CTGCAG 2 cut(s) 451, 786
PstNI CAGNNNCTG 2 cut(s) 455, 1025
RsaI GTAC 4 cut(s) 655, 728, 1075, 1097
RsaNI GTAC 4 cut(s) 654, 727, 1074, 1096
RseI CAYNNNNRTG 3 cut(s) 494, 609, 877
SaqAI TTAA 3 cut(s) 287, 876, 1079
SatI GCNGC 6 cut(s) 172, 175, 695, 815, 890, 1222
Sau3AI GATC 4 cut(s) 51, 160, 229, 921
SbfI CCTGCAGG 1 cut(s) 786
ScaI AGTACT 1 cut(s) 1075
ScrFI CCNGG 2 cut(s) 234, 1046
SdaI CCTGCAGG 1 cut(s) 786
SduI GDGCHC 1 cut(s) 616
SfaNI GCATC 3 cut(s) 328, 705, 1012
SfcI CTRYAG 4 cut(s) 375, 447, 456, 782
Sfr274I CTCGAG 1 cut(s) 915
SlaI CTCGAG 1 cut(s) 915
SmiMI CAYNNNNRTG 3 cut(s) 494, 609, 877
SmlI CTYRAG 2 cut(s) 89, 915
SmoI CTYRAG 2 cut(s) 89, 915
Sse8387I CCTGCAGG 1 cut(s) 786
Sse9I AATT 8 cut(s) 84, 380, 438, 588, 834, 1000, 1037, 1114
SseBI AGGCCT 1 cut(s) 780
SsiI CCGC 1 cut(s) 174
SspI AATATT 1 cut(s) 650
SspMI CTAG 7 cut(s) 314, 368, 776, 861, 992, 1119, 1140
StuI AGGCCT 1 cut(s) 780
StyD4I CCNGG 2 cut(s) 232, 1044
TaaI ACNGT 1 cut(s) 1135
TaqI TCGA 3 cut(s) 558, 916, 924
TasI AATT 8 cut(s) 84, 380, 438, 588, 834, 1000, 1037, 1114
TatI WGTACW 2 cut(s) 653, 1073
TauI GCSGC 1 cut(s) 177
TfiI GAWTC 2 cut(s) 452, 909
Tru1I TTAA 3 cut(s) 287, 876, 1079
Tru9I TTAA 3 cut(s) 287, 876, 1079
TscAI CASTG 1 cut(s) 282
TseI GCWGC 5 cut(s) 171, 694, 814, 889, 1221
TspDTI ATGAA 9 cut(s) 84, 102, 204, 282, 495, 506, 950, 986, 994
TspGWI ACGGA 1 cut(s) 752
TspRI CASTG 1 cut(s) 282
XapI RAATTY 4 cut(s) 84, 380, 1037, 1114
XbaI TCTAGA 1 cut(s) 860
XhoI CTCGAG 1 cut(s) 915
XmnI GAANNNNTTC 1 cut(s) 1135
XspI CTAG 7 cut(s) 314, 368, 776, 861, 992, 1119, 1140
ZrmI AGTACT 1 cut(s) 1075
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.