Prupe.1G342000_v2.0.a1

membrane protein At1g16860-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
N/A
Physical Location & Seq
Reverse (-)
32118674 .. 32120268
1595 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G342000.1_v2.0.a1.CDS.3

Sequence Viewer

Length: 825 bp
ATGCCTGGTCCAGCTTCCTTAAAGAAGTCAAATTCTGGACCGCTAAATAAGCATGGGAAACCTCTTAAGAAGTCGTCTGGTCCTCAATCTGGTGGGGTAACACCAACTGGCCGCCAAAACTCTGGACCTCTTCCTCCTGTTCTCCCTGCAACAGGTCTCATTACATCTGGACCCATTTCTTCTGGCCCACTAAATGCTTCTGGGGCCCCTCTTAAGGTATCTGGTCCTTTGGAGTCAATGGGATCAATAAAAGTACAAGGTTCCTCCATTGTTCACAATCAGGCCATTACTACTCTTAGCCAAGGTGCAGTCCACAATGCATATCTCCTCATTGTGGTTGTGATCCTTTTTGGTGCAGTTGCTACACTATTCACATGGAATACTTATTGGGGAAGACGAGCTATTATAGGTTACATCGCTAGTTATTCAGATTCTGAGCTGAGGACTGCAAAGAATGGTTTTCACAAGTTATTTCTGGTTCTGTTTTTGAAGGTGGTCACTTGTGGCAATCTGCCCCTTGAGTTGTCCTTTCAGAAGGTTCCTAGATGTGTGTATACATCTACAAGTTTGTACGAGTATCGAGGATGGGATTCAAAAGCTGCCAACCCTACACATCATCGCTTCACTTGGGGGCTCAGGTCCATAGAAAGGCGTGTGGTAGATTTCTACATCTCTGATTTCCAATCTGGGTTAAGAGCATTGGTTAAGACTGGCTCTGGAGCAAGGGTGACTCCATATGTTGATGATTCGATTGTCATTGATGTGAACCCTGAAAATGAAGAGCTGTCTCCAGAGTTTATTGGGAGAGAGGAACCTTTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

275

Amino Acids

29.57

Weight (kDa)

9.64

Isoelectric Point (pI)

34.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 554
AciI CCGC 2 cut(s) 41, 112
AclWI GGATC 2 cut(s) 250, 337
AcoI YGGCCR 1 cut(s) 109
AcsI RAATTY 1 cut(s) 31
AfaI GTAC 2 cut(s) 255, 572
AfiI CCNNNNNNNGG 5 cut(s) 89, 152, 214, 334, 648
AflII CTTAAG 2 cut(s) 65, 212
AgsI TTSAA 3 cut(s) 490, 594, 820
AjnI CCWGG 1 cut(s) 4
AluBI AGCT 5 cut(s) 14, 401, 439, 599, 784
AluI AGCT 5 cut(s) 14, 401, 439, 599, 784
Alw26I GTCTC 2 cut(s) 161, 792
AlwI GGATC 2 cut(s) 250, 337
AlwNI CAGNNNCTG 1 cut(s) 434
AoxI GGCC 4 cut(s) 109, 184, 204, 282
ApaI GGGCCC 1 cut(s) 208
ApeKI GCWGC 1 cut(s) 599
ApoI RAATTY 1 cut(s) 31
AsuHPI GGTGA 1 cut(s) 739
AvaII GGWCC 7 cut(s) 8, 38, 80, 125, 170, 224, 639
BaeGI GKGCMC 1 cut(s) 208
BanII GRGCYC 2 cut(s) 208, 636
BbsI GAAGAC 1 cut(s) 400
BbvCI CCTCAGC 1 cut(s) 440
BbvI GCAGC 1 cut(s) 586
BccI CCATC 1 cut(s) 579
BciT130I CCWGG 1 cut(s) 6
BcoDI GTCTC 2 cut(s) 161, 792
BfaI CTAG 2 cut(s) 420, 543
BfrI CTTAAG 2 cut(s) 65, 212
BisI GCNGC 2 cut(s) 112, 600
BlsI GCNGC 2 cut(s) 113, 601
Bme1390I CCNGG 1 cut(s) 6
Bme18I GGWCC 7 cut(s) 8, 38, 80, 125, 170, 224, 639
BmiI GGNNCC 7 cut(s) 172, 205, 206, 207, 262, 540, 813
BmrFI CCNGG 1 cut(s) 6
BpiI GAAGAC 1 cut(s) 400
BpmI CTGGAG 2 cut(s) 738, 774
Bpu10I CCTNAGC 2 cut(s) 440, 635
BpuEI CTTGAG 1 cut(s) 539
BsaI GGTCTC 1 cut(s) 161
BsaJI CCNNGG 1 cut(s) 301
BsaXI ACNNNNNCTCC 2 cut(s) 711, 741
Bsc4I CCNNNNNNNGG 5 cut(s) 89, 152, 214, 334, 648
Bse1I ACTGG 2 cut(s) 112, 715
BseBI CCWGG 1 cut(s) 6
BseDI CCNNGG 1 cut(s) 301
BseGI GGATG 1 cut(s) 590
BseLI CCNNNNNNNGG 5 cut(s) 89, 152, 214, 334, 648
BseMII CTCAG 3 cut(s) 426, 431, 649
BseNI ACTGG 2 cut(s) 112, 715
BseRI GAGGAG 1 cut(s) 317
BseSI GKGCMC 1 cut(s) 208
BseXI GCAGC 1 cut(s) 586
BsgI GTGCAG 2 cut(s) 327, 375
BshFI GGCC 4 cut(s) 111, 186, 206, 284
BslI CCNNNNNNNGG 5 cut(s) 89, 152, 214, 334, 648
BsmAI GTCTC 2 cut(s) 161, 792
BsnI GGCC 4 cut(s) 111, 186, 206, 284
Bso31I GGTCTC 1 cut(s) 161
Bsp120I GGGCCC 1 cut(s) 204
Bsp1286I GDGCHC 2 cut(s) 208, 636
Bsp143I GATC 2 cut(s) 242, 342
BspACI CCGC 2 cut(s) 41, 112
BspANI GGCC 4 cut(s) 111, 186, 206, 284
BspCNI CTCAG 3 cut(s) 427, 432, 648
BspLI GGNNCC 7 cut(s) 172, 205, 206, 207, 262, 540, 813
BspPI GGATC 2 cut(s) 250, 337
BspQI GCTCTTC 1 cut(s) 774
BspTI CTTAAG 2 cut(s) 65, 212
BspTNI GGTCTC 1 cut(s) 161
BsrI ACTGG 2 cut(s) 112, 715
BssECI CCNNGG 1 cut(s) 301
BssMI GATC 2 cut(s) 242, 342
BssNAI GTATAC 1 cut(s) 555
BssT1I CCWWGG 1 cut(s) 301
Bst1107I GTATAC 1 cut(s) 555
Bst2UI CCWGG 1 cut(s) 6
Bst6I CTCTTC 2 cut(s) 135, 774
BstAFI CTTAAG 2 cut(s) 65, 212
BstDEI CTNAG 4 cut(s) 296, 435, 440, 635
BstENI CCTNNNNNAGG 1 cut(s) 150
BstF5I GGATG 1 cut(s) 590
BstKTI GATC 2 cut(s) 245, 345
BstMAI GTCTC 2 cut(s) 161, 792
BstMBI GATC 2 cut(s) 242, 342
BstMWI GCNNNNNNNGC 2 cut(s) 49, 203
BstNI CCWGG 1 cut(s) 6
BstSCI CCNGG 1 cut(s) 4
BstSLI GKGCMC 1 cut(s) 208
BstV1I GCAGC 1 cut(s) 586
BstV2I GAAGAC 1 cut(s) 400
BstXI CCANNNNNNTGG 1 cut(s) 122
BstZ17I GTATAC 1 cut(s) 555
BsuRI GGCC 4 cut(s) 111, 186, 206, 284
BtgZI GCGATG 2 cut(s) 400, 602
BtsCI GGATG 1 cut(s) 590
CaiI CAGNNNCTG 1 cut(s) 434
Csp6I GTAC 2 cut(s) 254, 571
CviAII CATG 2 cut(s) 53, 375
CviQI GTAC 2 cut(s) 254, 571
DdeI CTNAG 4 cut(s) 296, 435, 440, 635
DpnI GATC 2 cut(s) 244, 344
DpnII GATC 2 cut(s) 242, 342
EaeI YGGCCR 1 cut(s) 109
Eam1104I CTCTTC 2 cut(s) 135, 774
EarI CTCTTC 2 cut(s) 135, 774
Eco130I CCWWGG 1 cut(s) 301
Eco24I GRGCYC 2 cut(s) 208, 636
Eco31I GGTCTC 1 cut(s) 161
Eco47I GGWCC 7 cut(s) 8, 38, 80, 125, 170, 224, 639
EcoNI CCTNNNNNAGG 1 cut(s) 150
EcoO109I RGGNCCY 2 cut(s) 204, 205
EcoRII CCWGG 1 cut(s) 4
EcoT14I CCWWGG 1 cut(s) 301
EcoT22I ATGCAT 1 cut(s) 322
EcoT38I GRGCYC 2 cut(s) 208, 636
ErhI CCWWGG 1 cut(s) 301
FaeI CATG 2 cut(s) 56, 378
FaiI YATR 8 cut(s) 54, 322, 376, 407, 555, 644, 736, 738
FatI CATG 2 cut(s) 52, 374
FauNDI CATATG 1 cut(s) 736
FblI GTMKAC 1 cut(s) 554
Fnu4HI GCNGC 2 cut(s) 112, 600
FokI GGATG 1 cut(s) 597
FriOI GRGCYC 2 cut(s) 208, 636
Fsp4HI GCNGC 2 cut(s) 112, 600
FspBI CTAG 2 cut(s) 420, 543
GluI GCNGC 2 cut(s) 112, 600
GsuI CTGGAG 2 cut(s) 738, 774
HaeIII GGCC 4 cut(s) 111, 186, 206, 284
Hin1II CATG 2 cut(s) 56, 378
HinfI GANTC 5 cut(s) 233, 431, 590, 730, 746
HphI GGTGA 1 cut(s) 739
Hpy166II GTNNAC 4 cut(s) 274, 313, 555, 766
Hpy188I TCNGA 4 cut(s) 430, 436, 534, 676
Hpy188III TCNNGA 5 cut(s) 36, 123, 168, 717, 791
Hpy8I GTNNAC 4 cut(s) 274, 313, 555, 766
HpyAV CCTTC 2 cut(s) 484, 529
HpyCH4V TGCA 5 cut(s) 149, 308, 320, 356, 449
HpyF10VI GCNNNNNNNGC 2 cut(s) 49, 203
HpyF3I CTNAG 4 cut(s) 296, 435, 440, 635
Hsp92II CATG 2 cut(s) 56, 378
Kzo9I GATC 2 cut(s) 242, 342
LguI GCTCTTC 1 cut(s) 774
LmnI GCTCC 1 cut(s) 719
Lsp1109I GCAGC 1 cut(s) 586
MaeI CTAG 2 cut(s) 420, 543
MaeIII GTNAC 4 cut(s) 97, 410, 496, 727
MalI GATC 2 cut(s) 244, 344
MboI GATC 2 cut(s) 242, 342
MboII GAAGA 4 cut(s) 122, 171, 405, 791
MhlI GDGCHC 2 cut(s) 208, 636
MluCI AATT 1 cut(s) 31
MlyI GAGTC 2 cut(s) 242, 724
Mph1103I ATGCAT 1 cut(s) 322
MseI TTAA 5 cut(s) 20, 66, 213, 692, 705
MslI CAYNNNNRTG 1 cut(s) 761
MspCI CTTAAG 2 cut(s) 65, 212
MspR9I CCNGG 1 cut(s) 6
MvaI CCWGG 1 cut(s) 6
MwoI GCNNNNNNNGC 2 cut(s) 49, 203
NdeI CATATG 1 cut(s) 736
NdeII GATC 2 cut(s) 242, 342
NlaIII CATG 2 cut(s) 56, 378
NlaIV GGNNCC 7 cut(s) 172, 205, 206, 207, 262, 540, 813
NmuCI GTSAC 2 cut(s) 496, 727
NsiI ATGCAT 1 cut(s) 322
PciSI GCTCTTC 1 cut(s) 774
PfeI GAWTC 3 cut(s) 431, 590, 746
PkrI GCNGC 2 cut(s) 113, 601
PleI GAGTC 2 cut(s) 241, 724
PpsI GAGTC 2 cut(s) 241, 724
Psp6I CCWGG 1 cut(s) 4
PspGI CCWGG 1 cut(s) 4
PspN4I GGNNCC 7 cut(s) 172, 205, 206, 207, 262, 540, 813
PspOMI GGGCCC 1 cut(s) 204
PstNI CAGNNNCTG 1 cut(s) 434
RsaI GTAC 2 cut(s) 255, 572
RsaNI GTAC 2 cut(s) 254, 571
RseI CAYNNNNRTG 1 cut(s) 761
SapI GCTCTTC 1 cut(s) 774
SaqAI TTAA 5 cut(s) 20, 66, 213, 692, 705
SatI GCNGC 2 cut(s) 112, 600
Sau3AI GATC 2 cut(s) 242, 342
SchI GAGTC 2 cut(s) 242, 724
ScrFI CCNGG 1 cut(s) 6
SduI GDGCHC 2 cut(s) 208, 636
SinI GGWCC 7 cut(s) 8, 38, 80, 125, 170, 224, 639
SmiMI CAYNNNNRTG 1 cut(s) 761
SmlI CTYRAG 3 cut(s) 65, 212, 518
SmoI CTYRAG 3 cut(s) 65, 212, 518
Sse9I AATT 1 cut(s) 31
SsiI CCGC 2 cut(s) 41, 112
SspMI CTAG 2 cut(s) 420, 543
StyD4I CCNGG 1 cut(s) 4
StyI CCWWGG 1 cut(s) 301
TaqI TCGA 2 cut(s) 580, 749
TasI AATT 1 cut(s) 31
TatI WGTACW 1 cut(s) 253
TauI GCSGC 1 cut(s) 114
TfiI GAWTC 3 cut(s) 431, 590, 746
Tru1I TTAA 5 cut(s) 20, 66, 213, 692, 705
Tru9I TTAA 5 cut(s) 20, 66, 213, 692, 705
TseFI GTSAC 2 cut(s) 496, 727
TseI GCWGC 1 cut(s) 599
Tsp45I GTSAC 2 cut(s) 496, 727
TspDTI ATGAA 1 cut(s) 792
Vha464I CTTAAG 2 cut(s) 65, 212
VpaK11BI GGWCC 7 cut(s) 8, 38, 80, 125, 170, 224, 639
XagI CCTNNNNNAGG 1 cut(s) 150
XapI RAATTY 1 cut(s) 31
XmiI GTMKAC 1 cut(s) 554
XspI CTAG 2 cut(s) 420, 543
Zsp2I ATGCAT 1 cut(s) 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.