Prupe.1G479800_v2.0.a1

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
N/A
Physical Location & Seq
Reverse (-)
40101607 .. 40104579
2973 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G479800.1_v2.0.a1.CDS.3

Sequence Viewer

Length: 1008 bp
ATGACAGAGAAAATCCAAGCAGAGGATTTGGGTCCCTTTCAGGGTGTGGGAACCTGCTATAGACCTAGAGCATTACAGCTCCACAACTGCCCTAATCTTCAGCTTAGGGGACTCACTCATGTTAATAGCTCAAAAGGTCACATTGGTATAAGTTACAGCCAAAATGTAACTCTCTCTCATCTCAATATAAGTGCTCCTGAATCAAGCCCTAACACTGATGGAATTGATCTCTACTCCGCAACAAATGTGAGCATCCATCAAAGTACCATTACAACTGGGGATGATTGCATTGCTATTAAGGAAATTTTTTCTTATATCAATATTACGGGGGATTATGTGTGGCCCAGGGCATGGAATAAGCACAGGACGGATAAGAGAAAAACTTATGTGAAACTCGTGGGAAGCCTTGGAGCAGGTGGAGCATATGAAAGAGTTGAAGAAGTGTTTGTGGGAAATTGTACTTTTAAAGGGTCTATGTATGGAGCAAGGATCAAGACCTGGCAGGGCCTGCCCACAGTCAGCTTTCTGTTTTTGGTTATTGTTTCTTCATCTAGCTTTGGCATTGGATATGGGCAGAATGACACTTATAATGTGCTTGACATTGGTGCCTTTGGAGATGGCGGAGCAACACAAGTCCTAAACATCCCACAAGGGAAAACTTACTTGCTCAAACCTCTCAAATTTGTGGGTCCTTGCAATGCAAACAAGGTTCAAATTGAGCAAATCAGAGTCACAGAGACCAAACATCCGATCATCATTGGCCAAAACTATGTAGACGATAACAGAAGAGTGAAAGTGAGCGATATAACATTTAGTGGCTTTGAAAGAGCTTCGACTAGCCAGCAAGCAACAACACTGAATTGTTGTAGCTTGGGCTGCACCAACATTGTCATGGATCATGTCAAATTAAGTTCCATGGTGCCTGGAAAGCCACTCAGATCTTTATGCAAGAATGCCAATGGAACATCAATCTCTACATCCCCTCATGTTGATTGCCTATCCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

336

Amino Acids

36.51

Weight (kDa)

8.83

Isoelectric Point (pI)

33.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 588
AarI CACCTGC 1 cut(s) 404
Acc36I ACCTGC 2 cut(s) 62, 404
AccB1I GGYRCC 2 cut(s) 605, 919
AccB7I CCANNNNNTGG 1 cut(s) 351
AccI GTMKAC 1 cut(s) 774
AciI CCGC 2 cut(s) 237, 621
AclWI GGATC 2 cut(s) 497, 903
AcoI YGGCCR 1 cut(s) 760
AcsI RAATTY 2 cut(s) 303, 680
AcuI CTGAAG 1 cut(s) 83
AfaI GTAC 2 cut(s) 265, 460
AfiI CCNNNNNNNGG 3 cut(s) 22, 41, 351
AgsI TTSAA 3 cut(s) 437, 713, 824
AjnI CCWGG 3 cut(s) 344, 497, 922
AluBI AGCT 7 cut(s) 79, 103, 129, 522, 555, 830, 870
AluI AGCT 7 cut(s) 79, 103, 129, 522, 555, 830, 870
Alw21I GWGCWC 1 cut(s) 196
Alw26I GTCTC 1 cut(s) 731
AlwI GGATC 2 cut(s) 497, 903
AlwNI CAGNNNCTG 1 cut(s) 508
AoxI GGCC 3 cut(s) 341, 505, 760
ApeKI GCWGC 1 cut(s) 876
ApoI RAATTY 2 cut(s) 303, 680
AspS9I GGNCC 4 cut(s) 32, 342, 505, 689
AvaII GGWCC 2 cut(s) 32, 689
BalI TGGCCA 1 cut(s) 762
BanI GGYRCC 2 cut(s) 605, 919
BauI CACGAG 1 cut(s) 395
Bbv12I GWGCWC 1 cut(s) 196
BbvI GCAGC 1 cut(s) 863
BccI CCATC 3 cut(s) 212, 264, 611
BciT130I CCWGG 3 cut(s) 346, 499, 924
BcoDI GTCTC 1 cut(s) 731
BfaI CTAG 3 cut(s) 66, 552, 837
BfmI CTRYAG 1 cut(s) 58
BfuAI ACCTGC 2 cut(s) 62, 404
BglII AGATCT 1 cut(s) 938
BisI GCNGC 1 cut(s) 877
BlsI GCNGC 1 cut(s) 878
Bme1390I CCNGG 3 cut(s) 346, 499, 924
Bme18I GGWCC 2 cut(s) 32, 689
BmgT120I GGNCC 4 cut(s) 32, 342, 505, 689
BmiI GGNNCC 6 cut(s) 33, 34, 52, 607, 690, 921
BmrFI CCNGG 3 cut(s) 346, 499, 924
BmrI ACTGGG 1 cut(s) 285
BmsI GCATC 1 cut(s) 261
BmuI ACTGGG 1 cut(s) 285
Bpu10I CCTNAGC 1 cut(s) 104
BsaI GGTCTC 1 cut(s) 731
BsaJI CCNNGG 4 cut(s) 344, 345, 406, 915
Bsc4I CCNNNNNNNGG 3 cut(s) 22, 41, 351
Bse1I ACTGG 1 cut(s) 280
Bse3DI GCAATG 2 cut(s) 288, 703
BseBI CCWGG 3 cut(s) 346, 499, 924
BseDI CCNNGG 4 cut(s) 344, 345, 406, 915
BseGI GGATG 5 cut(s) 252, 286, 642, 745, 977
BseLI CCNNNNNNNGG 3 cut(s) 22, 41, 351
BseMI GCAATG 2 cut(s) 288, 703
BseMII CTCAG 1 cut(s) 949
BseNI ACTGG 1 cut(s) 280
BseXI GCAGC 1 cut(s) 863
BsgI GTGCAG 1 cut(s) 862
BshFI GGCC 3 cut(s) 343, 507, 762
BshNI GGYRCC 2 cut(s) 605, 919
BsiHKAI GWGCWC 1 cut(s) 196
BslFI GGGAC 2 cut(s) 18, 123
BslI CCNNNNNNNGG 3 cut(s) 22, 41, 351
BsmAI GTCTC 1 cut(s) 731
BsmFI GGGAC 2 cut(s) 18, 123
BsmI GAATGC 1 cut(s) 958
BsnI GGCC 3 cut(s) 343, 507, 762
Bso31I GGTCTC 1 cut(s) 731
Bsp1286I GDGCHC 1 cut(s) 196
Bsp143I GATC 5 cut(s) 226, 489, 750, 895, 938
Bsp19I CCATGG 1 cut(s) 915
BspACI CCGC 2 cut(s) 237, 621
BspANI GGCC 3 cut(s) 343, 507, 762
BspCNI CTCAG 1 cut(s) 948
BspLI GGNNCC 6 cut(s) 33, 34, 52, 607, 690, 921
BspMI ACCTGC 2 cut(s) 62, 404
BspPI GGATC 2 cut(s) 497, 903
BspT107I GGYRCC 2 cut(s) 605, 919
BspTNI GGTCTC 1 cut(s) 731
BsrDI GCAATG 2 cut(s) 288, 703
BsrI ACTGG 1 cut(s) 280
BssECI CCNNGG 4 cut(s) 344, 345, 406, 915
BssMI GATC 5 cut(s) 226, 489, 750, 895, 938
BssSI CACGAG 1 cut(s) 395
BssT1I CCWWGG 2 cut(s) 406, 915
Bst2BI CACGAG 1 cut(s) 395
Bst2UI CCWGG 3 cut(s) 346, 499, 924
Bst4CI ACNGT 1 cut(s) 517
Bst6I CTCTTC 1 cut(s) 781
BstAPI GCANNNNNTGC 1 cut(s) 508
BstC8I GCNNGC 3 cut(s) 509, 842, 846
BstDEI CTNAG 2 cut(s) 104, 935
BstDSI CCRYGG 1 cut(s) 915
BstF5I GGATG 5 cut(s) 252, 286, 642, 745, 977
BstKTI GATC 5 cut(s) 229, 492, 753, 898, 941
BstMAI GTCTC 1 cut(s) 731
BstMBI GATC 5 cut(s) 226, 489, 750, 895, 938
BstMWI GCNNNNNNNGC 4 cut(s) 419, 508, 876, 928
BstNI CCWGG 3 cut(s) 346, 499, 924
BstSCI CCNGG 3 cut(s) 344, 497, 922
BstSFI CTRYAG 1 cut(s) 58
BstV1I GCAGC 1 cut(s) 863
BstX2I RGATCY 1 cut(s) 938
BstYI RGATCY 1 cut(s) 938
BsuRI GGCC 3 cut(s) 343, 507, 762
BtgI CCRYGG 1 cut(s) 915
BtsCI GGATG 5 cut(s) 252, 286, 642, 745, 977
BtsIMutI CAGTG 2 cut(s) 213, 854
BveI ACCTGC 2 cut(s) 62, 404
Cac8I GCNNGC 3 cut(s) 509, 842, 846
CaiI CAGNNNCTG 1 cut(s) 508
Cfr13I GGNCC 4 cut(s) 32, 342, 505, 689
Csp6I GTAC 2 cut(s) 264, 459
CviAII CATG 6 cut(s) 119, 351, 892, 899, 916, 986
CviQI GTAC 2 cut(s) 264, 459
DdeI CTNAG 2 cut(s) 104, 935
DpnI GATC 5 cut(s) 228, 491, 752, 897, 940
DpnII GATC 5 cut(s) 226, 489, 750, 895, 938
DraI TTTAAA 1 cut(s) 466
EaeI YGGCCR 1 cut(s) 760
Eam1104I CTCTTC 1 cut(s) 781
EarI CTCTTC 1 cut(s) 781
EciI GGCGGA 1 cut(s) 636
Eco130I CCWWGG 2 cut(s) 406, 915
Eco31I GGTCTC 1 cut(s) 731
Eco47I GGWCC 2 cut(s) 32, 689
Eco57I CTGAAG 1 cut(s) 83
EcoO109I RGGNCCY 3 cut(s) 32, 505, 689
EcoRII CCWGG 3 cut(s) 344, 497, 922
EcoT14I CCWWGG 2 cut(s) 406, 915
ErhI CCWWGG 2 cut(s) 406, 915
FaeI CATG 6 cut(s) 122, 354, 895, 902, 919, 989
FaqI GGGAC 2 cut(s) 18, 123
FatI CATG 6 cut(s) 118, 350, 891, 898, 915, 985
FauNDI CATATG 1 cut(s) 424
FblI GTMKAC 1 cut(s) 774
Fnu4HI GCNGC 1 cut(s) 877
FokI GGATG 5 cut(s) 239, 293, 629, 732, 964
Fsp4HI GCNGC 1 cut(s) 877
FspBI CTAG 3 cut(s) 66, 552, 837
GluI GCNGC 1 cut(s) 877
HaeIII GGCC 3 cut(s) 343, 507, 762
Hin1II CATG 6 cut(s) 122, 354, 895, 902, 919, 989
HinfI GANTC 3 cut(s) 111, 200, 729
Hpy166II GTNNAC 1 cut(s) 775
Hpy188I TCNGA 3 cut(s) 728, 750, 938
Hpy188III TCNNGA 2 cut(s) 197, 493
Hpy8I GTNNAC 1 cut(s) 775
HpyCH4III ACNGT 1 cut(s) 517
HpyCH4V TGCA 5 cut(s) 288, 696, 701, 879, 948
HpyF10VI GCNNNNNNNGC 4 cut(s) 419, 508, 876, 928
HpyF3I CTNAG 2 cut(s) 104, 935
Hsp92II CATG 6 cut(s) 122, 354, 895, 902, 919, 989
KflI GGGWCCC 1 cut(s) 32
Kzo9I GATC 5 cut(s) 226, 489, 750, 895, 938
LmnI GCTCC 6 cut(s) 84, 199, 410, 419, 482, 623
Lsp1109I GCAGC 1 cut(s) 863
LweI GCATC 1 cut(s) 261
MaeI CTAG 3 cut(s) 66, 552, 837
MaeIII GTNAC 4 cut(s) 137, 152, 166, 730
MalI GATC 5 cut(s) 228, 491, 752, 897, 940
MboI GATC 5 cut(s) 226, 489, 750, 895, 938
MboII GAAGA 4 cut(s) 89, 449, 537, 798
MflI RGATCY 1 cut(s) 938
MhlI GDGCHC 1 cut(s) 196
MlsI TGGCCA 1 cut(s) 762
MluCI AATT 7 cut(s) 222, 303, 454, 680, 714, 859, 905
MluNI TGGCCA 1 cut(s) 762
MlyI GAGTC 2 cut(s) 105, 738
MnlI CCTC 3 cut(s) 16, 684, 993
Mox20I TGGCCA 1 cut(s) 762
MscI TGGCCA 1 cut(s) 762
MseI TTAA 4 cut(s) 123, 297, 465, 908
MslI CAYNNNNRTG 1 cut(s) 890
Msp20I TGGCCA 1 cut(s) 762
MspR9I CCNGG 3 cut(s) 346, 499, 924
Mva1269I GAATGC 1 cut(s) 958
MvaI CCWGG 3 cut(s) 346, 499, 924
MwoI GCNNNNNNNGC 4 cut(s) 419, 508, 876, 928
NcoI CCATGG 1 cut(s) 915
NdeI CATATG 1 cut(s) 424
NdeII GATC 5 cut(s) 226, 489, 750, 895, 938
NlaIII CATG 6 cut(s) 122, 354, 895, 902, 919, 989
NlaIV GGNNCC 6 cut(s) 33, 34, 52, 607, 690, 921
NmuCI GTSAC 2 cut(s) 137, 730
PaqCI CACCTGC 1 cut(s) 404
PasI CCCWGGG 1 cut(s) 345
PctI GAATGC 1 cut(s) 958
PfeI GAWTC 1 cut(s) 200
PflMI CCANNNNNTGG 1 cut(s) 351
PkrI GCNGC 1 cut(s) 878
PleI GAGTC 2 cut(s) 105, 737
PpsI GAGTC 2 cut(s) 105, 737
PpuMI RGGWCCY 2 cut(s) 32, 689
PsiI TTATAA 1 cut(s) 588
Psp5II RGGWCCY 2 cut(s) 32, 689
Psp6I CCWGG 3 cut(s) 344, 497, 922
PspGI CCWGG 3 cut(s) 344, 497, 922
PspN4I GGNNCC 6 cut(s) 33, 34, 52, 607, 690, 921
PspPI GGNCC 4 cut(s) 32, 342, 505, 689
PspPPI RGGWCCY 2 cut(s) 32, 689
PstNI CAGNNNCTG 1 cut(s) 508
PsuI RGATCY 1 cut(s) 938
RsaI GTAC 2 cut(s) 265, 460
RsaNI GTAC 2 cut(s) 264, 459
RseI CAYNNNNRTG 1 cut(s) 890
SaqAI TTAA 4 cut(s) 123, 297, 465, 908
SatI GCNGC 1 cut(s) 877
Sau3AI GATC 5 cut(s) 226, 489, 750, 895, 938
Sau96I GGNCC 4 cut(s) 32, 342, 505, 689
SchI GAGTC 2 cut(s) 105, 738
ScrFI CCNGG 3 cut(s) 346, 499, 924
SduI GDGCHC 1 cut(s) 196
SfaNI GCATC 1 cut(s) 261
SfcI CTRYAG 1 cut(s) 58
SinI GGWCC 2 cut(s) 32, 689
SmiMI CAYNNNNRTG 1 cut(s) 890
Sse9I AATT 7 cut(s) 222, 303, 454, 680, 714, 859, 905
SsiI CCGC 2 cut(s) 237, 621
SspI AATATT 1 cut(s) 322
SspMI CTAG 3 cut(s) 66, 552, 837
StyD4I CCNGG 3 cut(s) 344, 497, 922
StyI CCWWGG 2 cut(s) 406, 915
TaaI ACNGT 1 cut(s) 517
TaqI TCGA 1 cut(s) 833
TasI AATT 7 cut(s) 222, 303, 454, 680, 714, 859, 905
TatI WGTACW 1 cut(s) 458
TfiI GAWTC 1 cut(s) 200
Tru1I TTAA 4 cut(s) 123, 297, 465, 908
Tru9I TTAA 4 cut(s) 123, 297, 465, 908
TscAI CASTG 2 cut(s) 220, 861
TseFI GTSAC 2 cut(s) 137, 730
TseI GCWGC 1 cut(s) 876
Tsp45I GTSAC 2 cut(s) 137, 730
TspDTI ATGAA 2 cut(s) 441, 537
TspGWI ACGGA 1 cut(s) 383
TspRI CASTG 2 cut(s) 220, 861
Van91I CCANNNNNTGG 1 cut(s) 351
VpaK11BI GGWCC 2 cut(s) 32, 689
XapI RAATTY 2 cut(s) 303, 680
XcmI CCANNNNNNNNNTGG 1 cut(s) 889
XmiI GTMKAC 1 cut(s) 774
XspI CTAG 3 cut(s) 66, 552, 837
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.