Prupe.1G481400_v2.0.a1

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
N/A
Physical Location & Seq
Forward (+)
40187908 .. 40189006
1099 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G481400.1_v2.0.a1.CDS.3

Sequence Viewer

Length: 966 bp
ATGCTTTCCCCAACGCCATTTTCTCTAGTCTGCGCCATTCTTCTCTGTTTGCCTCTCACCATAATCTTCACCATCACAAGCCCAACTGCCACCACAACCACCACCATCCCCACCCAAATCTCTCAATCCCCAAAACTCACCAAAACCTACCAAAAAATCAACCTAATTTCACCGCCAAAATCCCTCCCATTAGATGACGACTCGCTTTTCCGCCTAGCCGCCCGTGTCAATTCCAGCCCGCCTTGCTCCGATCGCCCCAAAATCGCCTTCCTCTTCCTAACCACCACCCTTCTCCCCTTCTCCCCTTCTCCCATCTCTGGGAACGCTTCTTCAACTAAACTCAAAAACCCATTTCTCTATCTACCGACTCGTCGTTTGCTTGCCCACGAGCTCCTCAATGACCCCAAGAACGCAATGTTCGCGTGTCTCTCTCCTTCCTGCATCCTCATCCAGTCATTTAACTTCACATACCAAACCCTATCCCGATCGAAAAAGAGCTTCATCGAGATTCTGGACAACGAGATCTGGGCGACGCGTGGGGAGGACGCGATGCTGCCACAGGTGAAGCTGGAGAAGTTTCGGATCTGGTCCCAATTCTGGATCCTGAAACGGAAGCACGTGAGGGTAGTTGTGGGTGACCACTGGCTCTGGTCCAAGTTCAAGCTACCATGTCAGCATTGGGACACGTGTTACCGGGAGGAAAATTACTTTCCTACCCTCCTAAACATGAGAGACCCAGGCAAGTGCGTGCCCGTTACCCTCACACACCGCGGGAGATTCGATGGCCACCCCTGCACATATGAGGCCTCCGAGGTGGGGCTCGATTTGATCACCGCCCTGAGAAATGACAGGGTGAAGTACGGTGTCGAGAAGGAGAGCAGCAACGGCTCTGATTGGTCCGTGATGGAAAGGCGAGATCCATTTCTGTTCACGAGAAAGTTCCCACCGGATGCGATTGACCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

322

Amino Acids

36.69

Weight (kDa)

9.26

Isoelectric Point (pI)

49.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 422, 535, 548, 771
AciI CCGC 7 cut(s) 173, 211, 219, 239, 769, 771, 834
AclWI GGATC 4 cut(s) 590, 595, 608, 911
AcoI YGGCCR 1 cut(s) 784
AcvI CACGTG 2 cut(s) 619, 687
AfaI GTAC 1 cut(s) 860
AfiI CCNNNNNNNGG 4 cut(s) 317, 318, 597, 816
AflIII ACRYGT 3 cut(s) 533, 684, 686
AgsI TTSAA 2 cut(s) 333, 661
AjnI CCWGG 1 cut(s) 736
AjuI GAANNNNNNNTTGG 3 cut(s) 36, 251, 283
AluBI AGCT 4 cut(s) 391, 498, 568, 664
AluI AGCT 4 cut(s) 391, 498, 568, 664
Alw21I GWGCWC 1 cut(s) 393
Alw26I GTCTC 2 cut(s) 431, 726
AlwI GGATC 4 cut(s) 590, 595, 608, 911
AoxI GGCC 2 cut(s) 784, 804
ApeKI GCWGC 2 cut(s) 553, 879
ArsI GACNNNNNNTTYG 2 cut(s) 358, 390
AspLEI GCGC 1 cut(s) 35
AspS9I GGNCC 3 cut(s) 588, 651, 897
AsuC2I CCSGG 1 cut(s) 695
AsuHPI GGTGA 8 cut(s) 49, 61, 130, 162, 574, 647, 823, 865
AvaII GGWCC 3 cut(s) 588, 651, 897
BaeGI GKGCMC 1 cut(s) 753
BalI TGGCCA 1 cut(s) 786
BamHI GGATCC 1 cut(s) 600
BanII GRGCYC 2 cut(s) 393, 822
BauI CACGAG 2 cut(s) 386, 931
BbrPI CACGTG 2 cut(s) 619, 687
Bbv12I GWGCWC 1 cut(s) 393
BbvI GCAGC 2 cut(s) 540, 891
BccI CCATC 5 cut(s) 80, 113, 320, 776, 898
BceAI ACGGC 1 cut(s) 901
BciT130I CCWGG 1 cut(s) 738
BclI TGATCA 1 cut(s) 828
BcnI CCSGG 1 cut(s) 695
BcoDI GTCTC 2 cut(s) 431, 726
BfaI CTAG 2 cut(s) 26, 215
BglII AGATCT 1 cut(s) 522
BisI GCNGC 3 cut(s) 219, 554, 880
BlsI GCNGC 3 cut(s) 220, 555, 881
Bme1390I CCNGG 2 cut(s) 695, 738
Bme18I GGWCC 3 cut(s) 588, 651, 897
BmgT120I GGNCC 3 cut(s) 588, 651, 897
BmiI GGNNCC 2 cut(s) 590, 602
BmrFI CCNGG 2 cut(s) 695, 738
BmsI GCATC 3 cut(s) 450, 540, 940
BpmI CTGGAG 1 cut(s) 590
BpuMI CCSGG 1 cut(s) 695
BsaAI YACGTR 2 cut(s) 619, 687
BsaI GGTCTC 1 cut(s) 726
BsaJI CCNNGG 3 cut(s) 736, 769, 810
BsaWI WCCGGW 1 cut(s) 946
Bsc4I CCNNNNNNNGG 4 cut(s) 317, 318, 597, 816
Bse1I ACTGG 2 cut(s) 451, 647
Bse3DI GCAATG 1 cut(s) 420
BseBI CCWGG 1 cut(s) 738
BseDI CCNNGG 3 cut(s) 736, 769, 810
BseGI GGATG 4 cut(s) 105, 441, 447, 955
BseLI CCNNNNNNNGG 4 cut(s) 317, 318, 597, 816
BseMI GCAATG 1 cut(s) 420
BseMII CTCAG 1 cut(s) 830
BseNI ACTGG 2 cut(s) 451, 647
BseRI GAGGAG 1 cut(s) 383
BseSI GKGCMC 1 cut(s) 753
BseXI GCAGC 2 cut(s) 540, 891
BsgI GTGCAG 1 cut(s) 778
Bsh1236I CGCG 4 cut(s) 422, 535, 548, 771
Bsh1285I CGRYCG 2 cut(s) 253, 488
BshFI GGCC 2 cut(s) 786, 806
BsiEI CGRYCG 2 cut(s) 253, 488
BsiHKAI GWGCWC 1 cut(s) 393
BsiSI CCGG 2 cut(s) 694, 947
BslFI GGGAC 2 cut(s) 574, 695
BslI CCNNNNNNNGG 4 cut(s) 317, 318, 597, 816
BsmAI GTCTC 2 cut(s) 431, 726
BsmFI GGGAC 2 cut(s) 574, 695
BsnI GGCC 2 cut(s) 786, 806
Bso31I GGTCTC 1 cut(s) 726
Bsp1286I GDGCHC 3 cut(s) 393, 753, 822
Bsp143I GATC 7 cut(s) 250, 485, 522, 582, 600, 828, 916
BspACI CCGC 7 cut(s) 173, 211, 219, 239, 769, 771, 834
BspANI GGCC 2 cut(s) 786, 806
BspCNI CTCAG 1 cut(s) 831
BspFNI CGCG 4 cut(s) 422, 535, 548, 771
BspLI GGNNCC 2 cut(s) 590, 602
BspPI GGATC 4 cut(s) 590, 595, 608, 911
BspTNI GGTCTC 1 cut(s) 726
BsrDI GCAATG 1 cut(s) 420
BsrI ACTGG 2 cut(s) 451, 647
BssECI CCNNGG 3 cut(s) 736, 769, 810
BssMI GATC 7 cut(s) 250, 485, 522, 582, 600, 828, 916
BssSI CACGAG 2 cut(s) 386, 931
Bst2BI CACGAG 2 cut(s) 386, 931
Bst2UI CCWGG 1 cut(s) 738
Bst4CI ACNGT 2 cut(s) 863, 962
Bst6I CTCTTC 1 cut(s) 278
BstBAI YACGTR 2 cut(s) 619, 687
BstC8I GCNNGC 3 cut(s) 239, 381, 749
BstDEI CTNAG 1 cut(s) 839
BstDSI CCRYGG 1 cut(s) 769
BstEII GGTNACC 1 cut(s) 635
BstF5I GGATG 4 cut(s) 105, 441, 447, 955
BstFNI CGCG 4 cut(s) 422, 535, 548, 771
BstHHI GCGC 1 cut(s) 35
BstKTI GATC 7 cut(s) 253, 488, 525, 585, 603, 831, 919
BstMAI GTCTC 2 cut(s) 431, 726
BstMBI GATC 7 cut(s) 250, 485, 522, 582, 600, 828, 916
BstMCI CGRYCG 2 cut(s) 253, 488
BstMWI GCNNNNNNNGC 5 cut(s) 243, 252, 419, 792, 885
BstNI CCWGG 1 cut(s) 738
BstPI GGTNACC 1 cut(s) 635
BstSCI CCNGG 2 cut(s) 693, 736
BstSLI GKGCMC 1 cut(s) 753
BstUI CGCG 4 cut(s) 422, 535, 548, 771
BstV1I GCAGC 2 cut(s) 540, 891
BstX2I RGATCY 4 cut(s) 522, 582, 600, 916
BstYI RGATCY 4 cut(s) 522, 582, 600, 916
BsuRI GGCC 2 cut(s) 786, 806
BtgI CCRYGG 1 cut(s) 769
BtgZI GCGATG 1 cut(s) 563
BtsCI GGATG 4 cut(s) 105, 441, 447, 955
BtsIMutI CAGTG 1 cut(s) 640
Cac8I GCNNGC 3 cut(s) 239, 381, 749
CfoI GCGC 1 cut(s) 35
Cfr13I GGNCC 3 cut(s) 588, 651, 897
Cfr42I CCGCGG 1 cut(s) 772
CseI GACGC 2 cut(s) 541, 554
Csp6I GTAC 1 cut(s) 859
CviAII CATG 2 cut(s) 669, 727
CviQI GTAC 1 cut(s) 859
DdeI CTNAG 1 cut(s) 839
DpnI GATC 7 cut(s) 252, 487, 524, 584, 602, 830, 918
DpnII GATC 7 cut(s) 250, 485, 522, 582, 600, 828, 916
EaeI YGGCCR 1 cut(s) 784
Eam1104I CTCTTC 1 cut(s) 278
EarI CTCTTC 1 cut(s) 278
EciI GGCGGA 1 cut(s) 200
Ecl136II GAGCTC 1 cut(s) 391
Eco147I AGGCCT 1 cut(s) 806
Eco24I GRGCYC 2 cut(s) 393, 822
Eco31I GGTCTC 1 cut(s) 726
Eco47I GGWCC 3 cut(s) 588, 651, 897
Eco53kI GAGCTC 1 cut(s) 391
Eco72I CACGTG 2 cut(s) 619, 687
Eco91I GGTNACC 1 cut(s) 635
EcoICRI GAGCTC 1 cut(s) 391
EcoO65I GGTNACC 1 cut(s) 635
EcoRII CCWGG 1 cut(s) 736
EcoT38I GRGCYC 2 cut(s) 393, 822
FaeI CATG 2 cut(s) 672, 730
FaiI YATR 6 cut(s) 62, 469, 670, 728, 799, 801
FaqI GGGAC 2 cut(s) 574, 695
FatI CATG 2 cut(s) 668, 726
FauI CCCGC 2 cut(s) 246, 764
FauNDI CATATG 1 cut(s) 799
FbaI TGATCA 1 cut(s) 828
Fnu4HI GCNGC 3 cut(s) 219, 554, 880
FokI GGATG 4 cut(s) 92, 428, 434, 962
FriOI GRGCYC 2 cut(s) 393, 822
Fsp4HI GCNGC 3 cut(s) 219, 554, 880
FspBI CTAG 2 cut(s) 26, 215
GlaI GCGC 1 cut(s) 34
GluI GCNGC 3 cut(s) 219, 554, 880
GsuI CTGGAG 1 cut(s) 590
HaeIII GGCC 2 cut(s) 786, 806
HapII CCGG 2 cut(s) 694, 947
HgaI GACGC 2 cut(s) 541, 554
HhaI GCGC 1 cut(s) 35
Hin1II CATG 2 cut(s) 672, 730
Hin6I GCGC 1 cut(s) 33
HinP1I GCGC 1 cut(s) 33
HinfI GANTC 4 cut(s) 200, 367, 508, 777
HpaII CCGG 2 cut(s) 694, 947
HphI GGTGA 8 cut(s) 49, 61, 130, 162, 574, 647, 823, 865
Hpy166II GTNNAC 1 cut(s) 930
Hpy188I TCNGA 4 cut(s) 250, 582, 811, 892
Hpy188III TCNNGA 7 cut(s) 483, 505, 512, 598, 604, 868, 931
Hpy8I GTNNAC 1 cut(s) 930
Hpy99I CGWCG 2 cut(s) 375, 535
HpyAV CCTTC 6 cut(s) 277, 299, 307, 315, 444, 865
HpyCH4III ACNGT 2 cut(s) 863, 962
HpyCH4IV ACGT 2 cut(s) 618, 686
HpyCH4V TGCA 2 cut(s) 441, 795
HpyF10VI GCNNNNNNNGC 5 cut(s) 243, 252, 419, 792, 885
HpyF3I CTNAG 1 cut(s) 839
HpySE526I ACGT 2 cut(s) 618, 686
Hsp92II CATG 2 cut(s) 672, 730
HspAI GCGC 1 cut(s) 33
Ksp22I TGATCA 1 cut(s) 828
KspI CCGCGG 1 cut(s) 772
Kzo9I GATC 7 cut(s) 250, 485, 522, 582, 600, 828, 916
LmnI GCTCC 2 cut(s) 251, 396
Lsp1109I GCAGC 2 cut(s) 540, 891
LweI GCATC 3 cut(s) 450, 540, 940
MaeI CTAG 2 cut(s) 26, 215
MaeII ACGT 2 cut(s) 618, 686
MaeIII GTNAC 3 cut(s) 635, 689, 754
MalI GATC 7 cut(s) 252, 487, 524, 584, 602, 830, 918
MboI GATC 7 cut(s) 250, 485, 522, 582, 600, 828, 916
MboII GAAGA 4 cut(s) 32, 58, 265, 321
MflI RGATCY 4 cut(s) 522, 582, 600, 916
MhlI GDGCHC 3 cut(s) 393, 753, 822
MlsI TGGCCA 1 cut(s) 786
MluCI AATT 4 cut(s) 165, 229, 593, 703
MluI ACGCGT 1 cut(s) 533
MluNI TGGCCA 1 cut(s) 786
MlyI GAGTC 2 cut(s) 194, 361
Mox20I TGGCCA 1 cut(s) 786
MscI TGGCCA 1 cut(s) 786
MseI TTAA 1 cut(s) 459
Msp20I TGGCCA 1 cut(s) 786
MspA1I CMGCKG 1 cut(s) 771
MspI CCGG 2 cut(s) 694, 947
MspR9I CCNGG 2 cut(s) 695, 738
MvaI CCWGG 1 cut(s) 738
MvnI CGCG 4 cut(s) 422, 535, 548, 771
MwoI GCNNNNNNNGC 5 cut(s) 243, 252, 419, 792, 885
NciI CCSGG 1 cut(s) 695
NdeI CATATG 1 cut(s) 799
NdeII GATC 7 cut(s) 250, 485, 522, 582, 600, 828, 916
NlaIII CATG 2 cut(s) 672, 730
NlaIV GGNNCC 2 cut(s) 590, 602
NmuCI GTSAC 1 cut(s) 635
PceI AGGCCT 1 cut(s) 806
PfeI GAWTC 2 cut(s) 508, 777
PkrI GCNGC 3 cut(s) 220, 555, 881
Ple19I CGATCG 2 cut(s) 253, 488
PleI GAGTC 2 cut(s) 194, 361
PmaCI CACGTG 2 cut(s) 619, 687
PmlI CACGTG 2 cut(s) 619, 687
PpsI GAGTC 2 cut(s) 194, 361
Ppu21I YACGTR 2 cut(s) 619, 687
Psp124BI GAGCTC 1 cut(s) 393
Psp6I CCWGG 1 cut(s) 736
PspCI CACGTG 2 cut(s) 619, 687
PspEI GGTNACC 1 cut(s) 635
PspGI CCWGG 1 cut(s) 736
PspN4I GGNNCC 2 cut(s) 590, 602
PspPI GGNCC 3 cut(s) 588, 651, 897
PsuI RGATCY 4 cut(s) 522, 582, 600, 916
PvuI CGATCG 2 cut(s) 253, 488
RsaI GTAC 1 cut(s) 860
RsaNI GTAC 1 cut(s) 859
SacI GAGCTC 1 cut(s) 393
SacII CCGCGG 1 cut(s) 772
SaqAI TTAA 1 cut(s) 459
SatI GCNGC 3 cut(s) 219, 554, 880
Sau3AI GATC 7 cut(s) 250, 485, 522, 582, 600, 828, 916
Sau96I GGNCC 3 cut(s) 588, 651, 897
SchI GAGTC 2 cut(s) 194, 361
ScrFI CCNGG 2 cut(s) 695, 738
SduI GDGCHC 3 cut(s) 393, 753, 822
SfaNI GCATC 3 cut(s) 450, 540, 940
Sfr303I CCGCGG 1 cut(s) 772
SgrBI CCGCGG 1 cut(s) 772
SinI GGWCC 3 cut(s) 588, 651, 897
Sse9I AATT 4 cut(s) 165, 229, 593, 703
SseBI AGGCCT 1 cut(s) 806
SsiI CCGC 7 cut(s) 173, 211, 219, 239, 769, 771, 834
SspMI CTAG 2 cut(s) 26, 215
SstI GAGCTC 1 cut(s) 393
StuI AGGCCT 1 cut(s) 806
StyD4I CCNGG 2 cut(s) 693, 736
TaaI ACNGT 2 cut(s) 863, 962
TaiI ACGT 2 cut(s) 621, 689
TaqI TCGA 5 cut(s) 488, 504, 780, 822, 867
TasI AATT 4 cut(s) 165, 229, 593, 703
TauI GCSGC 1 cut(s) 221
TfiI GAWTC 2 cut(s) 508, 777
Tru1I TTAA 1 cut(s) 459
Tru9I TTAA 1 cut(s) 459
TscAI CASTG 1 cut(s) 647
TseFI GTSAC 1 cut(s) 635
TseI GCWGC 2 cut(s) 553, 879
Tsp45I GTSAC 1 cut(s) 635
TspDTI ATGAA 1 cut(s) 490
TspGWI ACGGA 2 cut(s) 625, 889
TspRI CASTG 1 cut(s) 647
VpaK11BI GGWCC 3 cut(s) 588, 651, 897
XcmI CCANNNNNNNNNTGG 1 cut(s) 675
XspI CTAG 2 cut(s) 26, 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.