Prupe.1G497300_v2.0.a1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
N/A
Physical Location & Seq
Forward (+)
41194943 .. 41197579
2637 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G497300.1_v2.0.a1.CDS.5

Sequence Viewer

Length: 1176 bp
ATGGAGTCTCCCAGAATTACTATCAAAGTTATCATTTCTCTATTTTGCTCTTATATGCTTTTGTCACATTATATCACCACTTTGAGCCCTGCCTTGGCTTTGGAAGACTGTCACTTTCCAGCAATCTTTAACTTTGGAGACTCAAATTCAGATACTGGTGGAATGGCTGCGGCCATTTATCGACCACCACCACCCTCTGGGAAAACCCATTTTCATACGCCAGCGGGAAGATTCTCAGATGGCAGGCTCATAATCGATTTTCTTGCCAAGAGTCTTGGTCACCCCTTTCTAAGCGCATATCTGGATTCAATTGGGACCAACTTCTCGCATGGTGCAAATTTTGCCACTGCATCTTCCACCATCAGATTGCCAGATCCCATTATACCAGCTCCTGGAGGATTTAGTCCCTTCACCCTCAATATTCAATGCTCGCAGTTCTTGCAACTAAAATCCAGATCACAACTCATAAGGCATCGAGGGGGAATATTTGCAAGTTTGATGCCCAAGAAGAAGTATTTTGCCAAAGCCTTATACACATTTGACATTGGCCAGAATGACCTTCCAGAAGGGTTTTTTGGTAATTTGACTGTTCAAGAAGTCAATGCATCTGTTCCTAATATAGTCAGTACGTTCTCAGCAAATATTAAGAAAATATATGATTCGGGAGGGAGATCATTTTGGATACACAACACTGGGCCAATTGGGTGTATCCCTTACATCTTAGTTAATTTCCCAGCTCAAAAGGATGAGGTTGGCTGTGCAAAGTTATACAATGAAGTAGCTCAGTATTTTAACCACAAGCTGAAGGAGGCCACAGTTCAACTTAGGAAGGATCTACCTTTGGCTGCAATAACCTATGTAGACATATATTCAGTCAAATACTCTCTTTACAAGGAACCCCAAAAATATAGGTTTGAGCAACCACTTGTTTCTTGTTGTGGCACTGGTGGCAAGTACAACTTTAACAGTAAAACTGTTGAATGTGGAAGAACAGTTACAGTTAATGGAAGACAAATATTTGCTGACTCATGCAAACGTCCTTATGTTAAAGTGAACTGGGATGGGATTCATTATACTGATGCAGCTGCCAAGATTATTTTTGATAAAATTTCAACCGGAGCATTTTCCGATCCACCCCTTCCTTTGAAGCAAGCATGTCACAGGAGTTTGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005488 GO:0006950 GO:0006952 GO:0008150 GO:0016787 GO:0016788 GO:0019863 GO:0019865 GO:0044877 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

392

Amino Acids

43.28

Weight (kDa)

9.08

Isoelectric Point (pI)

44.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 197, 392
AccI GTMKAC 1 cut(s) 861
AciI CCGC 2 cut(s) 170, 224
AclWI GGATC 3 cut(s) 368, 840, 1124
AcoI YGGCCR 2 cut(s) 171, 547
AcsI RAATTY 3 cut(s) 145, 337, 1107
AcuI CTGAAG 1 cut(s) 824
AfaI GTAC 2 cut(s) 628, 956
AfiI CCNNNNNNNGG 3 cut(s) 94, 197, 392
AgsI TTSAA 7 cut(s) 309, 425, 593, 821, 980, 1113, 1147
AjnI CCWGG 1 cut(s) 391
AjuI GAANNNNNNNTTGG 2 cut(s) 558, 590
AluBI AGCT 5 cut(s) 389, 737, 782, 802, 1085
AluI AGCT 5 cut(s) 389, 737, 782, 802, 1085
Alw26I GTCTC 2 cut(s) 12, 132
AlwI GGATC 3 cut(s) 368, 840, 1124
AlwNI CAGNNNCTG 2 cut(s) 155, 392
AoxI GGCC 4 cut(s) 171, 547, 695, 810
ApeKI GCWGC 4 cut(s) 167, 845, 1082, 1085
ApoI RAATTY 3 cut(s) 145, 337, 1107
AspLEI GCGC 1 cut(s) 296
AspS9I GGNCC 2 cut(s) 315, 695
AsuHPI GGTGA 3 cut(s) 67, 272, 403
AvaII GGWCC 1 cut(s) 315
BalI TGGCCA 1 cut(s) 549
BanII GRGCYC 1 cut(s) 89
BarI GAAGNNNNNNTAC 2 cut(s) 979, 1011
BbsI GAAGAC 2 cut(s) 111, 1015
BbvI GCAGC 4 cut(s) 154, 832, 1072, 1094
BccI CCATC 3 cut(s) 233, 368, 1055
BcgI CGANNNNNNTGC 2 cut(s) 245, 279
BciT130I CCWGG 1 cut(s) 393
BciVI GTATCC 2 cut(s) 675, 719
BcoDI GTCTC 2 cut(s) 12, 132
BfuI GTATCC 2 cut(s) 675, 719
BisI GCNGC 5 cut(s) 168, 171, 846, 1083, 1086
BlsI GCNGC 5 cut(s) 169, 172, 847, 1084, 1087
Bme1390I CCNGG 1 cut(s) 393
Bme18I GGWCC 1 cut(s) 315
BmgT120I GGNCC 2 cut(s) 315, 695
BmiI GGNNCC 2 cut(s) 316, 897
BmrFI CCNGG 1 cut(s) 393
BmrI ACTGGG 2 cut(s) 702, 1066
BmsI GCATC 5 cut(s) 359, 481, 489, 614, 1069
BmuI ACTGGG 2 cut(s) 702, 1066
BpiI GAAGAC 2 cut(s) 111, 1015
BpmI CTGGAG 1 cut(s) 414
Bsa29I ATCGAT 1 cut(s) 255
BsaJI CCNNGG 1 cut(s) 93
BsaWI WCCGGW 1 cut(s) 1115
Bsc4I CCNNNNNNNGG 3 cut(s) 94, 197, 392
Bse1I ACTGG 4 cut(s) 160, 697, 949, 1061
BseBI CCWGG 1 cut(s) 393
BseCI ATCGAT 1 cut(s) 255
BseDI CCNNGG 1 cut(s) 93
BseGI GGATG 2 cut(s) 751, 1066
BseLI CCNNNNNNNGG 3 cut(s) 94, 197, 392
BseMII CTCAG 3 cut(s) 249, 648, 797
BseNI ACTGG 4 cut(s) 160, 697, 949, 1061
BseXI GCAGC 4 cut(s) 154, 832, 1072, 1094
BseYI CCCAGC 1 cut(s) 733
BshFI GGCC 4 cut(s) 173, 549, 697, 812
BshVI ATCGAT 1 cut(s) 255
BsiSI CCGG 1 cut(s) 1116
BslFI GGGAC 2 cut(s) 328, 390
BslI CCNNNNNNNGG 3 cut(s) 94, 197, 392
BsmAI GTCTC 2 cut(s) 12, 132
BsmFI GGGAC 2 cut(s) 328, 390
BsnI GGCC 4 cut(s) 173, 549, 697, 812
Bsp1286I GDGCHC 1 cut(s) 89
Bsp143I GATC 5 cut(s) 373, 455, 671, 832, 1129
BspACI CCGC 2 cut(s) 170, 224
BspANI GGCC 4 cut(s) 173, 549, 697, 812
BspCNI CTCAG 3 cut(s) 248, 647, 796
BspDI ATCGAT 1 cut(s) 255
BspLI GGNNCC 2 cut(s) 316, 897
BspPI GGATC 3 cut(s) 368, 840, 1124
BsrI ACTGG 4 cut(s) 160, 697, 949, 1061
BssECI CCNNGG 1 cut(s) 93
BssMI GATC 5 cut(s) 373, 455, 671, 832, 1129
BssT1I CCWWGG 1 cut(s) 93
Bst2UI CCWGG 1 cut(s) 393
Bst4CI ACNGT 7 cut(s) 110, 589, 817, 968, 976, 994, 1000
BstAPI GCANNNNNTGC 2 cut(s) 341, 439
BstC8I GCNNGC 4 cut(s) 222, 245, 431, 1152
BstDEI CTNAG 6 cut(s) 235, 290, 634, 721, 783, 824
BstEII GGTNACC 1 cut(s) 278
BstF5I GGATG 2 cut(s) 751, 1066
BstHHI GCGC 1 cut(s) 296
BstKTI GATC 5 cut(s) 376, 458, 674, 835, 1132
BstMAI GTCTC 2 cut(s) 12, 132
BstMBI GATC 5 cut(s) 373, 455, 671, 832, 1129
BstMWI GCNNNNNNNGC 3 cut(s) 341, 439, 948
BstNI CCWGG 1 cut(s) 393
BstNSI RCATGY 1 cut(s) 1158
BstPI GGTNACC 1 cut(s) 278
BstSCI CCNGG 1 cut(s) 391
BstV1I GCAGC 4 cut(s) 154, 832, 1072, 1094
BstV2I GAAGAC 2 cut(s) 111, 1015
BstX2I RGATCY 2 cut(s) 373, 832
BstYI RGATCY 2 cut(s) 373, 832
Bsu15I ATCGAT 1 cut(s) 255
BsuI GTATCC 2 cut(s) 675, 719
BsuRI GGCC 4 cut(s) 173, 549, 697, 812
BsuTUI ATCGAT 1 cut(s) 255
BtsCI GGATG 2 cut(s) 751, 1066
BtsI GCAGTG 1 cut(s) 345
BtsIMutI CAGTG 3 cut(s) 345, 690, 942
Cac8I GCNNGC 4 cut(s) 222, 245, 431, 1152
CaiI CAGNNNCTG 2 cut(s) 155, 392
CfoI GCGC 1 cut(s) 296
Cfr13I GGNCC 2 cut(s) 315, 695
ClaI ATCGAT 1 cut(s) 255
Csp6I GTAC 2 cut(s) 627, 955
CviAII CATG 3 cut(s) 329, 1029, 1155
CviQI GTAC 2 cut(s) 627, 955
DdeI CTNAG 6 cut(s) 235, 290, 634, 721, 783, 824
DpnI GATC 5 cut(s) 375, 457, 673, 834, 1131
DpnII GATC 5 cut(s) 373, 455, 671, 832, 1129
EaeI YGGCCR 2 cut(s) 171, 547
Eco130I CCWWGG 1 cut(s) 93
Eco24I GRGCYC 1 cut(s) 89
Eco47I GGWCC 1 cut(s) 315
Eco57I CTGAAG 1 cut(s) 824
Eco91I GGTNACC 1 cut(s) 278
EcoO65I GGTNACC 1 cut(s) 278
EcoRII CCWGG 1 cut(s) 391
EcoT14I CCWWGG 1 cut(s) 93
EcoT22I ATGCAT 1 cut(s) 607
EcoT38I GRGCYC 1 cut(s) 89
ErhI CCWWGG 1 cut(s) 93
FaeI CATG 3 cut(s) 332, 1032, 1158
FalI AAGNNNNNCTT 2 cut(s) 944, 976
FaqI GGGAC 2 cut(s) 328, 390
FatI CATG 3 cut(s) 328, 1028, 1154
FauI CCCGC 1 cut(s) 217
FblI GTMKAC 1 cut(s) 861
Fnu4HI GCNGC 5 cut(s) 168, 171, 846, 1083, 1086
FokI GGATG 2 cut(s) 758, 1073
FriOI GRGCYC 1 cut(s) 89
Fsp4HI GCNGC 5 cut(s) 168, 171, 846, 1083, 1086
GlaI GCGC 1 cut(s) 295
GluI GCNGC 5 cut(s) 168, 171, 846, 1083, 1086
GsaI CCCAGC 1 cut(s) 737
GsuI CTGGAG 1 cut(s) 414
HaeIII GGCC 4 cut(s) 173, 549, 697, 812
HapII CCGG 1 cut(s) 1116
HhaI GCGC 1 cut(s) 296
Hin1II CATG 3 cut(s) 332, 1032, 1158
Hin6I GCGC 1 cut(s) 294
HinP1I GCGC 1 cut(s) 294
HinfI GANTC 8 cut(s) 5, 140, 231, 271, 305, 659, 1025, 1066
HpaII CCGG 1 cut(s) 1116
HphI GGTGA 3 cut(s) 67, 272, 403
Hpy166II GTNNAC 2 cut(s) 862, 1054
Hpy188I TCNGA 4 cut(s) 151, 238, 365, 1129
Hpy188III TCNNGA 5 cut(s) 302, 453, 563, 593, 663
Hpy8I GTNNAC 2 cut(s) 862, 1054
HpyAV CCTTC 6 cut(s) 418, 560, 569, 799, 823, 1148
HpyCH4III ACNGT 7 cut(s) 110, 589, 817, 968, 976, 994, 1000
HpyCH4IV ACGT 2 cut(s) 629, 1036
HpyCH4V TGCA 9 cut(s) 335, 350, 442, 491, 605, 761, 848, 1032, 1082
HpyF10VI GCNNNNNNNGC 3 cut(s) 341, 439, 948
HpyF3I CTNAG 6 cut(s) 235, 290, 634, 721, 783, 824
HpySE526I ACGT 2 cut(s) 629, 1036
Hsp92II CATG 3 cut(s) 332, 1032, 1158
HspAI GCGC 1 cut(s) 294
Kzo9I GATC 5 cut(s) 373, 455, 671, 832, 1129
LmnI GCTCC 2 cut(s) 394, 1118
Lsp1109I GCAGC 4 cut(s) 154, 832, 1072, 1094
LweI GCATC 5 cut(s) 359, 481, 489, 614, 1069
MaeII ACGT 2 cut(s) 629, 1036
MaeIII GTNAC 5 cut(s) 63, 110, 278, 994, 1157
MalI GATC 5 cut(s) 375, 457, 673, 834, 1131
MboI GATC 5 cut(s) 373, 455, 671, 832, 1129
MboII GAAGA 6 cut(s) 116, 240, 345, 520, 999, 1020
MfeI CAATTG 2 cut(s) 309, 699
MflI RGATCY 2 cut(s) 373, 832
MhlI GDGCHC 1 cut(s) 89
MlsI TGGCCA 1 cut(s) 549
MluCI AATT 8 cut(s) 15, 145, 309, 337, 580, 699, 727, 1107
MluNI TGGCCA 1 cut(s) 549
MlyI GAGTC 4 cut(s) 14, 134, 280, 1019
MnlI CCTC 7 cut(s) 205, 389, 425, 470, 659, 742, 802
Mox20I TGGCCA 1 cut(s) 549
Mph1103I ATGCAT 1 cut(s) 607
MscI TGGCCA 1 cut(s) 549
MseI TTAA 7 cut(s) 129, 645, 726, 792, 963, 1002, 1047
Msp20I TGGCCA 1 cut(s) 549
MspA1I CMGCKG 2 cut(s) 224, 1085
MspI CCGG 1 cut(s) 1116
MspR9I CCNGG 1 cut(s) 393
MunI CAATTG 2 cut(s) 309, 699
MvaI CCWGG 1 cut(s) 393
MwoI GCNNNNNNNGC 3 cut(s) 341, 439, 948
NdeII GATC 5 cut(s) 373, 455, 671, 832, 1129
NlaIII CATG 3 cut(s) 332, 1032, 1158
NlaIV GGNNCC 2 cut(s) 316, 897
NmuCI GTSAC 4 cut(s) 63, 110, 278, 1157
NsiI ATGCAT 1 cut(s) 607
NspI RCATGY 1 cut(s) 1158
PfeI GAWTC 4 cut(s) 231, 305, 659, 1066
PflMI CCANNNNNTGG 2 cut(s) 197, 392
PfoI TCCNGGA 1 cut(s) 391
PkrI GCNGC 5 cut(s) 169, 172, 847, 1084, 1087
PleI GAGTC 4 cut(s) 13, 134, 279, 1019
PpsI GAGTC 4 cut(s) 13, 134, 279, 1019
Psp6I CCWGG 1 cut(s) 391
PspEI GGTNACC 1 cut(s) 278
PspFI CCCAGC 1 cut(s) 733
PspGI CCWGG 1 cut(s) 391
PspN4I GGNNCC 2 cut(s) 316, 897
PspPI GGNCC 2 cut(s) 315, 695
PstNI CAGNNNCTG 2 cut(s) 155, 392
PsuI RGATCY 2 cut(s) 373, 832
PvuII CAGCTG 1 cut(s) 1085
RsaI GTAC 2 cut(s) 628, 956
RsaNI GTAC 2 cut(s) 627, 955
SaqAI TTAA 7 cut(s) 129, 645, 726, 792, 963, 1002, 1047
SatI GCNGC 5 cut(s) 168, 171, 846, 1083, 1086
Sau3AI GATC 5 cut(s) 373, 455, 671, 832, 1129
Sau96I GGNCC 2 cut(s) 315, 695
SchI GAGTC 4 cut(s) 14, 134, 280, 1019
ScrFI CCNGG 1 cut(s) 393
SduI GDGCHC 1 cut(s) 89
SfaNI GCATC 5 cut(s) 359, 481, 489, 614, 1069
SinI GGWCC 1 cut(s) 315
Sse9I AATT 8 cut(s) 15, 145, 309, 337, 580, 699, 727, 1107
SsiI CCGC 2 cut(s) 170, 224
SspI AATATT 4 cut(s) 421, 486, 643, 1017
StyD4I CCNGG 1 cut(s) 391
StyI CCWWGG 1 cut(s) 93
TaaI ACNGT 7 cut(s) 110, 589, 817, 968, 976, 994, 1000
TaiI ACGT 2 cut(s) 632, 1039
TaqI TCGA 3 cut(s) 181, 255, 475
TasI AATT 8 cut(s) 15, 145, 309, 337, 580, 699, 727, 1107
TatI WGTACW 1 cut(s) 954
TauI GCSGC 1 cut(s) 173
TfiI GAWTC 4 cut(s) 231, 305, 659, 1066
Tru1I TTAA 7 cut(s) 129, 645, 726, 792, 963, 1002, 1047
Tru9I TTAA 7 cut(s) 129, 645, 726, 792, 963, 1002, 1047
TscAI CASTG 3 cut(s) 352, 697, 949
TseFI GTSAC 4 cut(s) 63, 110, 278, 1157
TseI GCWGC 4 cut(s) 167, 845, 1082, 1085
Tsp45I GTSAC 4 cut(s) 63, 110, 278, 1157
TspDTI ATGAA 3 cut(s) 203, 789, 1058
TspRI CASTG 3 cut(s) 352, 697, 949
Van91I CCANNNNNTGG 2 cut(s) 197, 392
VpaK11BI GGWCC 1 cut(s) 315
XapI RAATTY 3 cut(s) 145, 337, 1107
XceI RCATGY 1 cut(s) 1158
XmiI GTMKAC 1 cut(s) 861
Zsp2I ATGCAT 1 cut(s) 607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.