Prupe.1G532900_v2.0.a1

Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. Functions as Fe-S scaffold, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Essential for embryo development

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
N/A
Physical Location & Seq
Forward (+)
43569017 .. 43573023
4007 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G532900.1_v2.0.a1.CDS.2

Sequence Viewer

Length: 1302 bp
ATGAACCGCATCGCTCCCTCTCTCTCTCCCTCTCGCCTTCTCTTGCTCACCGTCTCTCTGTCCTCGTCGCGCTCTCTCTCTTGCTTTCAACTTGGTCGGTCTTTCTCAGCTCAGGTTGGAACTCCAATTTGTACTGTTGATATTGCAGAAAAGGGGCTGAAGGTATCCGTTAAGGCATTTACAGTAAAGCCAAGCCAATACTTAAAAGGAAAAGCGAGACCCCCAATCGTCATGGAGAATGGTGAAATTCCCGAAAACGCCAATGAACATTGTCCAGGTCCTCAGTCCGATTCTGCTGGAAAATCTGATGCATGCGAAGGATGCCCAAATCAACAAATTTGTGCTACTGCTCCAAAGGGGCCTGACCCAGACGTGGTTGCAATTGCAGAAAGGATGGCTACTGTAAAGCATAAGATACTGGTTTTATCAGGAAAAGGTGGAGTTGGCAAGAGCACATTCTCTGCCCAACTTGCATTTGCACTAGCTGCTATGGACTTCCAGGTGGGTCTTCTTGACATTGACATTTGTGGCCCGAGCATCCCAAAGATGCTTGGCCTAGAAGGCCAAGAAATCCACCAGAGCAACCTTGGCTGGTCTCCAGTCTATGTTGAGTCCAACCTTGGGGTCATGTCAATTGGGTTCATGCTTCCAGAACCTGATGAAGCTGTTATATGGAGGGGACCGCGCAAAAATGCGCTCATTAAGCAATTCTTGAAGGATGTTTATTGGGGTGAGCTTGATTTTCTAGTAGTTGATGCTCCTCCTGGGACCTCAGATGAGCACATTTCAATTGTTCAATACCTCGATGCTGGTTCAATAGATGGTGCAATCATAGTCACCACTCCACAACAGGTCTCCCTGATTGATGTTCGGAAAGAAGTAAGTTTCTGCAAGAAAGTTGGGGTTCAGGTCCTTGGGATTGTTGAGAACATGAGTGGCTTGTGCCAGCCAGTGATGGATTTCAAATTTTTGAGGATGACAGAGACCGGTGAGCAAATAGACGTCACAGAATGGGCAAGGGAGCATCTGAAAGAGAGAACACCAGAATTTTTAAACATAATAGCTTGCACTGAAGTGTTTGATAGTAGTGGTGGTGGAGCCGAAAGAATGTGCAGGGAAATGGGGGTGCCTTTTCTGGGGAAGGTACCATTGGACCCCCAGCTTTGCAAGGCAGCTGAAGAAGGTAGATCTTGTTTTGTTGATCAGAAGTGCAGGGTGAGTGCACCTGCACTGAAGAAAATCATAGATAAAATGATGGAAAATCCGGGATTATCTAGAGTGTTGGTAGACAACGATGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0042802 GO:0042803 GO:0044424 GO:0044444 GO:0044464 GO:0046983 GO:0048037 GO:0051536 GO:0051540
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ParA

Protein Analysis

434

Amino Acids

46.79

Weight (kDa)

5.38

Isoelectric Point (pI)

37.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1234
AatII GACGTC 1 cut(s) 1005
Acc36I ACCTGC 1 cut(s) 1234
Acc65I GGTACC 1 cut(s) 1144
AccB1I GGYRCC 2 cut(s) 1126, 1144
AccI GTMKAC 1 cut(s) 1287
AccII CGCG 2 cut(s) 70, 685
AciI CCGC 2 cut(s) 7, 683
AcsI RAATTY 4 cut(s) 246, 336, 965, 1046
AcuI CTGAAG 4 cut(s) 179, 1092, 1197, 1253
AcyI GRCGYC 1 cut(s) 1002
AfaI GTAC 2 cut(s) 133, 1146
AfiI CCNNNNNNNGG 3 cut(s) 373, 621, 1136
AgeI ACCGGT 1 cut(s) 986
AgsI TTSAA 6 cut(s) 89, 715, 789, 797, 816, 964
AjiI CACGTC 1 cut(s) 373
AjnI CCWGG 3 cut(s) 274, 498, 763
AjuI GAANNNNNNNTTGG 2 cut(s) 1133, 1165
AleI CACNNNNGTG 1 cut(s) 1073
AluBI AGCT 7 cut(s) 110, 485, 665, 736, 1064, 1162, 1175
AluI AGCT 7 cut(s) 110, 485, 665, 736, 1064, 1162, 1175
Alw21I GWGCWC 3 cut(s) 455, 783, 1225
Alw26I GTCTC 5 cut(s) 58, 211, 600, 859, 977
Alw44I GTGCAC 1 cut(s) 1221
AlwNI CAGNNNCTG 1 cut(s) 656
Ama87I CYCGRG 1 cut(s) 532
AoxI GGCC 4 cut(s) 359, 529, 553, 562
ApaLI GTGCAC 1 cut(s) 1221
ApeKI GCWGC 2 cut(s) 485, 1172
ApoI RAATTY 4 cut(s) 246, 336, 965, 1046
AsiGI ACCGGT 1 cut(s) 986
Asp718I GGTACC 1 cut(s) 1144
AspLEI GCGC 3 cut(s) 72, 687, 697
AspS9I GGNCC 7 cut(s) 278, 359, 530, 680, 768, 910, 1153
AsuC2I CCSGG 1 cut(s) 1266
AsuHPI GGTGA 6 cut(s) 40, 254, 743, 829, 1001, 1228
AvaI CYCGRG 1 cut(s) 532
AvaII GGWCC 5 cut(s) 278, 680, 768, 910, 1153
BaeGI GKGCMC 1 cut(s) 1225
BanI GGYRCC 2 cut(s) 1126, 1144
BbsI GAAGAC 1 cut(s) 500
Bbv12I GWGCWC 3 cut(s) 455, 783, 1225
BbvI GCAGC 2 cut(s) 472, 1184
BccI CCATC 4 cut(s) 388, 815, 949, 1249
BciT130I CCWGG 3 cut(s) 276, 500, 765
BciVI GTATCC 1 cut(s) 175
BclI TGATCA 1 cut(s) 1201
BcnI CCSGG 1 cut(s) 1266
BcoDI GTCTC 5 cut(s) 58, 211, 600, 859, 977
BfaI CTAG 4 cut(s) 482, 557, 746, 1275
BfuAI ACCTGC 1 cut(s) 1234
BfuI GTATCC 1 cut(s) 175
BglI GCCNNNNNGGC 1 cut(s) 561
BglII AGATCT 1 cut(s) 1187
BisI GCNGC 2 cut(s) 486, 1173
BlsI GCNGC 2 cut(s) 487, 1174
Bme1390I CCNGG 4 cut(s) 276, 500, 765, 1266
Bme18I GGWCC 5 cut(s) 278, 680, 768, 910, 1153
BmeT110I CYCGRG 1 cut(s) 532
BmgBI CACGTC 1 cut(s) 373
BmgT120I GGNCC 7 cut(s) 278, 359, 530, 680, 768, 910, 1153
BmiI GGNNCC 7 cut(s) 360, 681, 769, 1099, 1128, 1146, 1155
BmrFI CCNGG 4 cut(s) 276, 500, 765, 1266
BmsI GCATC 9 cut(s) 18, 298, 311, 537, 546, 745, 796, 1033, 1285
BpiI GAAGAC 1 cut(s) 500
BpmI CTGGAG 1 cut(s) 582
Bpu10I CCTNAGC 1 cut(s) 111
BpuMI CCSGG 1 cut(s) 1266
BsaHI GRCGYC 1 cut(s) 1002
BsaI GGTCTC 4 cut(s) 211, 600, 859, 977
BsaJI CCNNGG 4 cut(s) 586, 619, 764, 913
BsaWI WCCGGW 1 cut(s) 986
Bsc4I CCNNNNNNNGG 3 cut(s) 373, 621, 1136
Bse118I RCCGGY 1 cut(s) 986
Bse1I ACTGG 3 cut(s) 423, 599, 950
BseBI CCWGG 3 cut(s) 276, 500, 765
BseDI CCNNGG 4 cut(s) 586, 619, 764, 913
BseGI GGATG 5 cut(s) 326, 399, 537, 724, 981
BseLI CCNNNNNNNGG 3 cut(s) 373, 621, 1136
BseMII CTCAG 4 cut(s) 120, 125, 296, 786
BseNI ACTGG 3 cut(s) 423, 599, 950
BseRI GAGGAG 1 cut(s) 750
BseSI GKGCMC 1 cut(s) 1225
BseXI GCAGC 2 cut(s) 472, 1184
BseYI CCCAGC 1 cut(s) 1158
BsgI GTGCAG 3 cut(s) 1132, 1212, 1231
Bsh1236I CGCG 2 cut(s) 70, 685
BshFI GGCC 4 cut(s) 361, 531, 555, 564
BshNI GGYRCC 2 cut(s) 1126, 1144
BshTI ACCGGT 1 cut(s) 986
BsiHKAI GWGCWC 3 cut(s) 455, 783, 1225
BsiHKCI CYCGRG 1 cut(s) 532
BsiSI CCGG 2 cut(s) 987, 1265
BslFI GGGAC 2 cut(s) 693, 781
BslI CCNNNNNNNGG 3 cut(s) 373, 621, 1136
BsmAI GTCTC 5 cut(s) 58, 211, 600, 859, 977
BsmBI CGTCTC 1 cut(s) 58
BsmFI GGGAC 2 cut(s) 693, 781
BsnI GGCC 4 cut(s) 361, 531, 555, 564
Bso31I GGTCTC 4 cut(s) 211, 600, 859, 977
BsoBI CYCGRG 1 cut(s) 532
Bsp1286I GDGCHC 3 cut(s) 455, 783, 1225
Bsp143I GATC 2 cut(s) 1187, 1201
BspACI CCGC 2 cut(s) 7, 683
BspANI GGCC 4 cut(s) 361, 531, 555, 564
BspCNI CTCAG 4 cut(s) 119, 124, 295, 785
BspFNI CGCG 2 cut(s) 70, 685
BspLI GGNNCC 7 cut(s) 360, 681, 769, 1099, 1128, 1146, 1155
BspMI ACCTGC 1 cut(s) 1234
BspT107I GGYRCC 2 cut(s) 1126, 1144
BspTNI GGTCTC 4 cut(s) 211, 600, 859, 977
BsrFI RCCGGY 1 cut(s) 986
BsrI ACTGG 3 cut(s) 423, 599, 950
BssAI RCCGGY 1 cut(s) 986
BssECI CCNNGG 4 cut(s) 586, 619, 764, 913
BssMI GATC 2 cut(s) 1187, 1201
BssNI GRCGYC 1 cut(s) 1002
BssT1I CCWWGG 3 cut(s) 586, 619, 913
Bst2UI CCWGG 3 cut(s) 276, 500, 765
Bst4CI ACNGT 4 cut(s) 52, 136, 184, 403
BstACI GRCGYC 1 cut(s) 1002
BstAPI GCANNNNNTGC 1 cut(s) 485
BstC8I GCNNGC 3 cut(s) 313, 947, 1066
BstDEI CTNAG 4 cut(s) 106, 111, 282, 772
BstF5I GGATG 5 cut(s) 326, 399, 537, 724, 981
BstFNI CGCG 2 cut(s) 70, 685
BstHHI GCGC 3 cut(s) 72, 687, 697
BstKTI GATC 2 cut(s) 1190, 1204
BstMAI GTCTC 5 cut(s) 58, 211, 600, 859, 977
BstMBI GATC 2 cut(s) 1187, 1201
BstMWI GCNNNNNNNGC 6 cut(s) 321, 470, 485, 561, 588, 703
BstNI CCWGG 3 cut(s) 276, 500, 765
BstNSI RCATGY 1 cut(s) 315
BstSCI CCNGG 4 cut(s) 274, 498, 763, 1264
BstSLI GKGCMC 1 cut(s) 1225
BstUI CGCG 2 cut(s) 70, 685
BstV1I GCAGC 2 cut(s) 472, 1184
BstV2I GAAGAC 1 cut(s) 500
BstX2I RGATCY 1 cut(s) 1187
BstYI RGATCY 1 cut(s) 1187
BsuI GTATCC 1 cut(s) 175
BsuRI GGCC 4 cut(s) 361, 531, 555, 564
BtrI CACGTC 1 cut(s) 373
BtsCI GGATG 5 cut(s) 326, 399, 537, 724, 981
BtsIMutI CAGTG 3 cut(s) 957, 1068, 1229
BveI ACCTGC 1 cut(s) 1234
Cac8I GCNNGC 3 cut(s) 313, 947, 1066
CaiI CAGNNNCTG 1 cut(s) 656
CfoI GCGC 3 cut(s) 72, 687, 697
Cfr10I RCCGGY 1 cut(s) 986
Cfr13I GGNCC 7 cut(s) 278, 359, 530, 680, 768, 910, 1153
Csp6I GTAC 2 cut(s) 132, 1145
CspAI ACCGGT 1 cut(s) 986
CviAII CATG 5 cut(s) 232, 312, 628, 643, 931
CviQI GTAC 2 cut(s) 132, 1145
DdeI CTNAG 4 cut(s) 106, 111, 282, 772
DpnI GATC 2 cut(s) 1189, 1203
DpnII GATC 2 cut(s) 1187, 1201
DraI TTTAAA 1 cut(s) 1053
Eco130I CCWWGG 3 cut(s) 586, 619, 913
Eco31I GGTCTC 4 cut(s) 211, 600, 859, 977
Eco47I GGWCC 5 cut(s) 278, 680, 768, 910, 1153
Eco57I CTGAAG 4 cut(s) 179, 1092, 1197, 1253
Eco88I CYCGRG 1 cut(s) 532
EcoO109I RGGNCCY 4 cut(s) 278, 359, 768, 910
EcoRII CCWGG 3 cut(s) 274, 498, 763
EcoT14I CCWWGG 3 cut(s) 586, 619, 913
EcoT22I ATGCAT 2 cut(s) 313, 1300
ErhI CCWWGG 3 cut(s) 586, 619, 913
Esp3I CGTCTC 1 cut(s) 58
FaeI CATG 5 cut(s) 235, 315, 631, 646, 934
FalI AAGNNNNNCTT 2 cut(s) 695, 727
FaqI GGGAC 2 cut(s) 693, 781
FatI CATG 5 cut(s) 231, 311, 627, 642, 930
FbaI TGATCA 1 cut(s) 1201
FblI GTMKAC 1 cut(s) 1287
Fnu4HI GCNGC 2 cut(s) 486, 1173
FokI GGATG 5 cut(s) 333, 406, 524, 731, 988
Fsp4HI GCNGC 2 cut(s) 486, 1173
FspBI CTAG 4 cut(s) 482, 557, 746, 1275
GlaI GCGC 3 cut(s) 71, 686, 696
GluI GCNGC 2 cut(s) 486, 1173
GsaI CCCAGC 1 cut(s) 1162
GsuI CTGGAG 1 cut(s) 582
HaeIII GGCC 4 cut(s) 361, 531, 555, 564
HapII CCGG 2 cut(s) 987, 1265
HhaI GCGC 3 cut(s) 72, 687, 697
Hin1I GRCGYC 1 cut(s) 1002
Hin1II CATG 5 cut(s) 235, 315, 631, 646, 934
Hin6I GCGC 3 cut(s) 70, 685, 695
HinP1I GCGC 3 cut(s) 70, 685, 695
HinfI GANTC 2 cut(s) 290, 611
HpaII CCGG 2 cut(s) 987, 1265
HphI GGTGA 6 cut(s) 40, 254, 743, 829, 1001, 1228
Hpy166II GTNNAC 2 cut(s) 1223, 1288
Hpy188I TCNGA 6 cut(s) 289, 307, 775, 873, 1029, 1206
Hpy188III TCNNGA 6 cut(s) 251, 429, 512, 650, 712, 1275
Hpy8I GTNNAC 2 cut(s) 1223, 1288
Hpy99I CGWCG 1 cut(s) 70
HpyAV CCTTC 7 cut(s) 47, 154, 311, 554, 709, 1135, 1175
HpyCH4III ACNGT 4 cut(s) 52, 136, 184, 403
HpyCH4IV ACGT 2 cut(s) 372, 1002
HpyF10VI GCNNNNNNNGC 6 cut(s) 321, 470, 485, 561, 588, 703
HpyF3I CTNAG 4 cut(s) 106, 111, 282, 772
HpySE526I ACGT 2 cut(s) 372, 1002
Hsp92I GRCGYC 1 cut(s) 1002
Hsp92II CATG 5 cut(s) 235, 315, 631, 646, 934
HspAI GCGC 3 cut(s) 70, 685, 695
KpnI GGTACC 1 cut(s) 1148
Ksp22I TGATCA 1 cut(s) 1201
Kzo9I GATC 2 cut(s) 1187, 1201
LmnI GCTCC 5 cut(s) 19, 355, 763, 1021, 1097
Lsp1109I GCAGC 2 cut(s) 472, 1184
LweI GCATC 9 cut(s) 18, 298, 311, 537, 546, 745, 796, 1033, 1285
MaeI CTAG 4 cut(s) 482, 557, 746, 1275
MaeII ACGT 2 cut(s) 372, 1002
MaeIII GTNAC 2 cut(s) 835, 1003
MalI GATC 2 cut(s) 1189, 1203
MboI GATC 2 cut(s) 1187, 1201
MboII GAAGA 3 cut(s) 500, 1190, 1246
MfeI CAATTG 3 cut(s) 381, 633, 789
MflI RGATCY 1 cut(s) 1187
MhlI GDGCHC 3 cut(s) 455, 783, 1225
MluCI AATT 9 cut(s) 126, 246, 336, 381, 633, 707, 789, 965, 1046
MlyI GAGTC 1 cut(s) 620
MmeI TCCRAC 2 cut(s) 97, 639
MnlI CCTC 9 cut(s) 28, 40, 73, 291, 669, 771, 781, 812, 966
Mph1103I ATGCAT 2 cut(s) 313, 1300
MseI TTAA 4 cut(s) 171, 203, 702, 1052
MslI CAYNNNNRTG 1 cut(s) 1073
MspA1I CMGCKG 1 cut(s) 1175
MspI CCGG 2 cut(s) 987, 1265
MspR9I CCNGG 4 cut(s) 276, 500, 765, 1266
MunI CAATTG 3 cut(s) 381, 633, 789
MvaI CCWGG 3 cut(s) 276, 500, 765
MvnI CGCG 2 cut(s) 70, 685
MwoI GCNNNNNNNGC 6 cut(s) 321, 470, 485, 561, 588, 703
NciI CCSGG 1 cut(s) 1266
NdeII GATC 2 cut(s) 1187, 1201
NlaIII CATG 5 cut(s) 235, 315, 631, 646, 934
NlaIV GGNNCC 7 cut(s) 360, 681, 769, 1099, 1128, 1146, 1155
NmuCI GTSAC 2 cut(s) 835, 1003
NsiI ATGCAT 2 cut(s) 313, 1300
NspI RCATGY 1 cut(s) 315
OliI CACNNNNGTG 1 cut(s) 1073
PaeI GCATGC 1 cut(s) 315
PaqCI CACCTGC 1 cut(s) 1234
PfeI GAWTC 1 cut(s) 290
PfoI TCCNGGA 1 cut(s) 1264
PinAI ACCGGT 1 cut(s) 986
PkrI GCNGC 2 cut(s) 487, 1174
PleI GAGTC 1 cut(s) 619
PpsI GAGTC 1 cut(s) 619
PpuMI RGGWCCY 3 cut(s) 278, 768, 910
Psp5II RGGWCCY 3 cut(s) 278, 768, 910
Psp6I CCWGG 3 cut(s) 274, 498, 763
PspFI CCCAGC 1 cut(s) 1158
PspGI CCWGG 3 cut(s) 274, 498, 763
PspN4I GGNNCC 7 cut(s) 360, 681, 769, 1099, 1128, 1146, 1155
PspPI GGNCC 7 cut(s) 278, 359, 530, 680, 768, 910, 1153
PspPPI RGGWCCY 3 cut(s) 278, 768, 910
PstNI CAGNNNCTG 1 cut(s) 656
PsuI RGATCY 1 cut(s) 1187
PvuII CAGCTG 1 cut(s) 1175
RsaI GTAC 2 cut(s) 133, 1146
RsaNI GTAC 2 cut(s) 132, 1145
RseI CAYNNNNRTG 1 cut(s) 1073
SaqAI TTAA 4 cut(s) 171, 203, 702, 1052
SatI GCNGC 2 cut(s) 486, 1173
Sau3AI GATC 2 cut(s) 1187, 1201
Sau96I GGNCC 7 cut(s) 278, 359, 530, 680, 768, 910, 1153
SchI GAGTC 1 cut(s) 620
ScrFI CCNGG 4 cut(s) 276, 500, 765, 1266
SduI GDGCHC 3 cut(s) 455, 783, 1225
SfaNI GCATC 9 cut(s) 18, 298, 311, 537, 546, 745, 796, 1033, 1285
SfiI GGCCNNNNNGGCC 1 cut(s) 561
SinI GGWCC 5 cut(s) 278, 680, 768, 910, 1153
SmiMI CAYNNNNRTG 1 cut(s) 1073
SphI GCATGC 1 cut(s) 315
Sse9I AATT 9 cut(s) 126, 246, 336, 381, 633, 707, 789, 965, 1046
SsiI CCGC 2 cut(s) 7, 683
SspMI CTAG 4 cut(s) 482, 557, 746, 1275
StyD4I CCNGG 4 cut(s) 274, 498, 763, 1264
StyI CCWWGG 3 cut(s) 586, 619, 913
TaaI ACNGT 4 cut(s) 52, 136, 184, 403
TaiI ACGT 2 cut(s) 375, 1005
TaqI TCGA 1 cut(s) 804
TaqII GACCGA 1 cut(s) 87
TasI AATT 9 cut(s) 126, 246, 336, 381, 633, 707, 789, 965, 1046
TatI WGTACW 1 cut(s) 131
TfiI GAWTC 1 cut(s) 290
Tru1I TTAA 4 cut(s) 171, 203, 702, 1052
Tru9I TTAA 4 cut(s) 171, 203, 702, 1052
TscAI CASTG 3 cut(s) 957, 1075, 1236
TseFI GTSAC 2 cut(s) 835, 1003
TseI GCWGC 2 cut(s) 485, 1172
Tsp45I GTSAC 2 cut(s) 835, 1003
TspDTI ATGAA 4 cut(s) 17, 279, 631, 675
TspGWI ACGGA 1 cut(s) 157
TspRI CASTG 3 cut(s) 957, 1075, 1236
VneI GTGCAC 1 cut(s) 1221
VpaK11BI GGWCC 5 cut(s) 278, 680, 768, 910, 1153
XapI RAATTY 4 cut(s) 246, 336, 965, 1046
XbaI TCTAGA 1 cut(s) 1274
XceI RCATGY 1 cut(s) 315
XcmI CCANNNNNNNNNTGG 1 cut(s) 584
XmiI GTMKAC 1 cut(s) 1287
XspI CTAG 4 cut(s) 482, 557, 746, 1275
ZraI GACGTC 1 cut(s) 1003
Zsp2I ATGCAT 2 cut(s) 313, 1300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.