Prupe.1G536000_v2.0.a1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
N/A
Physical Location & Seq
Reverse (-)
43806529 .. 43807460
932 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G536000.1_v2.0.a1.CDS.3

Sequence Viewer

Length: 477 bp
ATGAAGCCTTATTCTTCGTCCTCATCATCCTCCTCCTCACAACGTTGGATGCACGATGTGTTCTTGAGCTTCAGAGGTGATGATACATGCAAAGGCTTCACGGGCCACCTCTACGTGACAATGGAAGGGGCTGGGATCAATGCGATTATTGACAACCAGCTAAGAAGAGGGGAAGAAATAACAGCGGAATTGGAGCAGGCAATCCAAGGGTCCAGGATCCCCATCATTGAGCTGGTAAAGATAATGGACTGTAGGAGAACACTTAGGCAAATAGTTTTGCCAATATTCTATGATGTTGATCCTTCGGATGTCAGGAAACCGACTGATACTGATCATAAGTTAGTGAGTTGGAGAGCTGCTCTTACTGAAGCTTCAAACTTGTCTGGCTGGGATCTTAGAAACGCGTTGGATAGGCACGAGGCAAAGTTTATCCGGAAAATTATCAAGGACATCACTATTCTGCTGAACACATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

159

Amino Acids

17.95

Weight (kDa)

6.91

Isoelectric Point (pI)

50.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 404
AccIII TCCGGA 1 cut(s) 432
AciI CCGC 1 cut(s) 185
AclI AACGTT 1 cut(s) 43
AclWI GGATC 5 cut(s) 143, 211, 224, 293, 399
AcuI CTGAAG 2 cut(s) 55, 387
AdeI CACNNNGTG 1 cut(s) 58
AflIII ACRYGT 1 cut(s) 402
AgsI TTSAA 1 cut(s) 375
AjnI CCWGG 1 cut(s) 212
AluBI AGCT 5 cut(s) 69, 160, 232, 356, 371
AluI AGCT 5 cut(s) 69, 160, 232, 356, 371
AlwI GGATC 5 cut(s) 143, 211, 224, 293, 399
Aor13HI TCCGGA 1 cut(s) 432
AoxI GGCC 1 cut(s) 103
ApeKI GCWGC 1 cut(s) 356
AspS9I GGNCC 2 cut(s) 103, 210
AsuHPI GGTGA 1 cut(s) 89
AvaII GGWCC 1 cut(s) 210
BamHI GGATCC 1 cut(s) 216
BauI CACGAG 1 cut(s) 416
BbvI GCAGC 1 cut(s) 343
BccI CCATC 1 cut(s) 230
BciT130I CCWGG 1 cut(s) 214
BclI TGATCA 1 cut(s) 331
BfmI CTRYAG 1 cut(s) 250
BisI GCNGC 1 cut(s) 357
BlsI GCNGC 1 cut(s) 358
Bme1390I CCNGG 1 cut(s) 214
Bme18I GGWCC 1 cut(s) 210
BmgT120I GGNCC 2 cut(s) 103, 210
BmiI GGNNCC 2 cut(s) 211, 218
BmrFI CCNGG 1 cut(s) 214
BmsI GCATC 1 cut(s) 39
BplI GAGNNNNNCTC 2 cut(s) 343, 375
BpuEI CTTGAG 1 cut(s) 85
BsaAI YACGTR 1 cut(s) 115
BsaBI GATNNNNATC 3 cut(s) 221, 297, 330
BsaJI CCNNGG 1 cut(s) 205
BsaWI WCCGGW 1 cut(s) 432
Bse8I GATNNNNATC 3 cut(s) 221, 297, 330
BseAI TCCGGA 1 cut(s) 432
BseBI CCWGG 1 cut(s) 214
BseDI CCNNGG 1 cut(s) 205
BseGI GGATG 3 cut(s) 26, 54, 313
BseJI GATNNNNATC 3 cut(s) 221, 297, 330
BseRI GAGGAG 2 cut(s) 22, 25
BseXI GCAGC 1 cut(s) 343
BseYI CCCAGC 2 cut(s) 131, 387
Bsh1236I CGCG 1 cut(s) 404
BshFI GGCC 1 cut(s) 105
BsiSI CCGG 1 cut(s) 433
BsnI GGCC 1 cut(s) 105
Bsp13I TCCGGA 1 cut(s) 432
Bsp143I GATC 5 cut(s) 135, 216, 298, 331, 391
BspACI CCGC 1 cut(s) 185
BspANI GGCC 1 cut(s) 105
BspEI TCCGGA 1 cut(s) 432
BspFNI CGCG 1 cut(s) 404
BspLI GGNNCC 2 cut(s) 211, 218
BspPI GGATC 5 cut(s) 143, 211, 224, 293, 399
BssECI CCNNGG 1 cut(s) 205
BssMI GATC 5 cut(s) 135, 216, 298, 331, 391
BssSI CACGAG 1 cut(s) 416
BssT1I CCWWGG 1 cut(s) 205
Bst2BI CACGAG 1 cut(s) 416
Bst2UI CCWGG 1 cut(s) 214
Bst4CI ACNGT 1 cut(s) 251
Bst6I CTCTTC 1 cut(s) 160
BstBAI YACGTR 1 cut(s) 115
BstC8I GCNNGC 1 cut(s) 198
BstDEI CTNAG 3 cut(s) 161, 263, 395
BstF5I GGATG 3 cut(s) 26, 54, 313
BstFNI CGCG 1 cut(s) 404
BstKTI GATC 5 cut(s) 138, 219, 301, 334, 394
BstMBI GATC 5 cut(s) 135, 216, 298, 331, 391
BstMWI GCNNNNNNNGC 1 cut(s) 102
BstNI CCWGG 1 cut(s) 214
BstNSI RCATGY 1 cut(s) 90
BstSCI CCNGG 1 cut(s) 212
BstSFI CTRYAG 1 cut(s) 250
BstUI CGCG 1 cut(s) 404
BstV1I GCAGC 1 cut(s) 343
BstX2I RGATCY 2 cut(s) 216, 391
BstYI RGATCY 2 cut(s) 216, 391
BsuRI GGCC 1 cut(s) 105
BtsCI GGATG 3 cut(s) 26, 54, 313
Cac8I GCNNGC 1 cut(s) 198
Cfr13I GGNCC 2 cut(s) 103, 210
CviAII CATG 1 cut(s) 87
DdeI CTNAG 3 cut(s) 161, 263, 395
DpnI GATC 5 cut(s) 137, 218, 300, 333, 393
DpnII GATC 5 cut(s) 135, 216, 298, 331, 391
DraIII CACNNNGTG 1 cut(s) 58
Eam1104I CTCTTC 1 cut(s) 160
EarI CTCTTC 1 cut(s) 160
Eco130I CCWWGG 1 cut(s) 205
Eco47I GGWCC 1 cut(s) 210
Eco57I CTGAAG 2 cut(s) 55, 387
EcoRII CCWGG 1 cut(s) 212
EcoT14I CCWWGG 1 cut(s) 205
ErhI CCWWGG 1 cut(s) 205
FaeI CATG 1 cut(s) 90
FaiI YATR 4 cut(s) 88, 291, 336, 475
FatI CATG 1 cut(s) 86
FbaI TGATCA 1 cut(s) 331
Fnu4HI GCNGC 1 cut(s) 357
FokI GGATG 3 cut(s) 13, 61, 320
Fsp4HI GCNGC 1 cut(s) 357
GluI GCNGC 1 cut(s) 357
GsaI CCCAGC 2 cut(s) 135, 391
HaeIII GGCC 1 cut(s) 105
HapII CCGG 1 cut(s) 433
Hin1II CATG 1 cut(s) 90
HindIII AAGCTT 1 cut(s) 369
HpaII CCGG 1 cut(s) 433
HphI GGTGA 1 cut(s) 89
Hpy188I TCNGA 2 cut(s) 74, 307
Hpy188III TCNNGA 3 cut(s) 64, 313, 433
HpyAV CCTTC 2 cut(s) 119, 312
HpyCH4III ACNGT 1 cut(s) 251
HpyCH4IV ACGT 2 cut(s) 43, 114
HpyCH4V TGCA 2 cut(s) 52, 90
HpyF10VI GCNNNNNNNGC 1 cut(s) 102
HpyF3I CTNAG 3 cut(s) 161, 263, 395
HpySE526I ACGT 2 cut(s) 43, 114
Hsp92II CATG 1 cut(s) 90
Kpn2I TCCGGA 1 cut(s) 432
Ksp22I TGATCA 1 cut(s) 331
Kzo9I GATC 5 cut(s) 135, 216, 298, 331, 391
LmnI GCTCC 1 cut(s) 193
Lsp1109I GCAGC 1 cut(s) 343
LweI GCATC 1 cut(s) 39
MaeII ACGT 2 cut(s) 43, 114
MaeIII GTNAC 1 cut(s) 115
MalI GATC 5 cut(s) 137, 218, 300, 333, 393
MboI GATC 5 cut(s) 135, 216, 298, 331, 391
MboII GAAGA 3 cut(s) 6, 177, 185
MflI RGATCY 2 cut(s) 216, 391
MluCI AATT 2 cut(s) 188, 438
MluI ACGCGT 1 cut(s) 402
MmeI TCCRAC 3 cut(s) 26, 329, 387
MnlI CCTC 8 cut(s) 31, 40, 43, 46, 68, 119, 161, 412
MroI TCCGGA 1 cut(s) 432
MspA1I CMGCKG 1 cut(s) 185
MspI CCGG 1 cut(s) 433
MspR9I CCNGG 1 cut(s) 214
MvaI CCWGG 1 cut(s) 214
MvnI CGCG 1 cut(s) 404
MwoI GCNNNNNNNGC 1 cut(s) 102
NdeII GATC 5 cut(s) 135, 216, 298, 331, 391
NlaIII CATG 1 cut(s) 90
NlaIV GGNNCC 2 cut(s) 211, 218
NmuCI GTSAC 1 cut(s) 115
NspI RCATGY 1 cut(s) 90
PfoI TCCNGGA 1 cut(s) 212
PkrI GCNGC 1 cut(s) 358
Ppu21I YACGTR 1 cut(s) 115
Psp1406I AACGTT 1 cut(s) 43
Psp6I CCWGG 1 cut(s) 212
PspFI CCCAGC 2 cut(s) 131, 387
PspGI CCWGG 1 cut(s) 212
PspN4I GGNNCC 2 cut(s) 211, 218
PspPI GGNCC 2 cut(s) 103, 210
PsuI RGATCY 2 cut(s) 216, 391
SatI GCNGC 1 cut(s) 357
Sau3AI GATC 5 cut(s) 135, 216, 298, 331, 391
Sau96I GGNCC 2 cut(s) 103, 210
ScrFI CCNGG 1 cut(s) 214
SetI ASST 9 cut(s) 46, 71, 79, 111, 117, 162, 234, 358, 373
SfaNI GCATC 1 cut(s) 39
SfcI CTRYAG 1 cut(s) 250
SinI GGWCC 1 cut(s) 210
SmlI CTYRAG 1 cut(s) 64
SmoI CTYRAG 1 cut(s) 64
Sse9I AATT 2 cut(s) 188, 438
SsiI CCGC 1 cut(s) 185
SspI AATATT 1 cut(s) 285
StyD4I CCNGG 1 cut(s) 212
StyI CCWWGG 1 cut(s) 205
TaaI ACNGT 1 cut(s) 251
TaiI ACGT 2 cut(s) 46, 117
TasI AATT 2 cut(s) 188, 438
TseFI GTSAC 1 cut(s) 115
TseI GCWGC 1 cut(s) 356
Tsp45I GTSAC 1 cut(s) 115
TspDTI ATGAA 1 cut(s) 17
VpaK11BI GGWCC 1 cut(s) 210
XceI RCATGY 1 cut(s) 90
XcmI CCANNNNNNNNNTGG 1 cut(s) 229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.