Prupe.1G563100_v2.0.a1

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
N/A
Physical Location & Seq
Reverse (-)
46011882 .. 46015416
3535 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G563100.1_v2.0.a1.CDS.5

Sequence Viewer

Length: 1443 bp
ATGGCATCTCAGCCATGGATGTGCATAGGCTTTTTAACTTCCATGCTTGTTCTCATGGGTGCAGTGGAGGCCCTTCCCATGGCTAATAAAATTGTGAGTTTGCCTGGGCAGCCACGTGTCAGCTTCCAACAATTTGCAGGCTACGTTGATGTAGACGACCACCCCCAGAGATCTCTGTTTTACTATTTTGTTGAAGCAGAGTCTCGCCCTGCTTCGAAACCGCTTGTTGTCTGGTTCAATGGAGGGCCTGGTTGTTCGTCGGTTGGAGAGGGCGCTTTCGTTGAACACGGTCCTTTTAAACCAAGTGGAGGGGCTACTCTAATAAAGAATCCATACAGTTGGAACACAGAAGCAAACATGTTGTACCTAGAGTCACCAGCCGGTGTTGGTTTCTCATTTGCTGCTGATAAGTCATTTTACGAAAATGTGAATGACAACATAACAGCACGGGACAGTTTAACATTTCTTCAGCGCTGGTTTGACAAATTTCCAGAATACAACCACAGAGATTTGTTTCTTGTAGGAGAAAGCTATGCCGGTCACTATGTGCCACAACTTGCACAGCTAATAATTCAGTCTGGTGTGAATTTCAACCTCAAGGGGATAGCTATAGGGAACCCTCTGCTGGAATTCAGTACTGATGTCAATGCGGAGGATTTGTTTTACTGGTCTCATGGGCTGATTTCTGATGCTGCTTATGGACTTCTCACTTCAGTCTGCAACAGTTCTCAGTTATTGAGAGAGACCATAGCAGGCTCTCCCTCTGATGCTTGTTCTGATGTAATCACTCAAGTTTCCCAAGAACTTAGCAACTATGTCGACAAATACTACGTTACCGGCGACATTTGTTTGTCGCCCCCCTTTCCAACAAAATTGGAAATTCTGAACCCGTTAAGGTCAAACTTTCGAACTTCAGCATTTCATCGTTCACGTGCAGAAGCCGGCAATTATAATCAGCAACTTACGGACAAGATAGATGCCTGTGTAGAGGAAGAAACAGTGACTTATTTAAACAGAAAAGATGTACAAAAGGCCCTTCATGCTAAGCTTGTAGGAGTCTCTAATTGGAGTTTGTGCAGCCAGGTTCTGCAATATGATATGCGAGATATGGAAATTCCTACCATTCCTATTGTCGGCTCACTTGTCAAATCTGGCATTCGTGTAATGGTTTATAGTGGAGATCAAGATTCAGTGGTTCCATTTTTCGGAAGTCGAAGTTTGATTAATGGATTGGCAACGCAGCTGGGTCTCAAGTCAACTGTGCCTTACAGAGCTTGGTTTGAGGGAAAGCAGGTTGGTGGGTGGACACAAGTTTATGATGATATCCTATCATTCGCCATCATAAGAGGAGCTTCACACACAGCTCCATCTACACAACCAAAGAGATCACTTGCGCTGTTTAAGTCATTTCTAGGAGGCAAACCACTGCCAGCCCATGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

481

Amino Acids

52.9

Weight (kDa)

5.64

Isoelectric Point (pI)

40.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 951
Acc36I ACCTGC 1 cut(s) 1282
AccI GTMKAC 2 cut(s) 153, 819
AciI CCGC 2 cut(s) 221, 650
AcsI RAATTY 5 cut(s) 485, 586, 629, 879, 1113
AcuI CTGAAG 3 cut(s) 452, 696, 897
AcvI CACGTG 2 cut(s) 116, 932
AdeI CACNNNGTG 1 cut(s) 547
AfaI GTAC 3 cut(s) 365, 637, 1026
AfeI AGCGCT 1 cut(s) 473
AfiI CCNNNNNNNGG 5 cut(s) 79, 308, 625, 1133, 1205
AflIII ACRYGT 2 cut(s) 115, 357
AgsI TTSAA 4 cut(s) 194, 238, 284, 592
AjnI CCWGG 3 cut(s) 103, 247, 1080
AluBI AGCT 9 cut(s) 123, 531, 565, 608, 1048, 1243, 1274, 1352, 1364
AluI AGCT 9 cut(s) 123, 531, 565, 608, 1048, 1243, 1274, 1352, 1364
Alw26I GTCTC 5 cut(s) 207, 675, 737, 1063, 1253
AlwNI CAGNNNCTG 1 cut(s) 1087
Aor51HI AGCGCT 1 cut(s) 473
AoxI GGCC 3 cut(s) 69, 245, 1032
ApeKI GCWGC 5 cut(s) 109, 401, 692, 1077, 1240
ApoI RAATTY 5 cut(s) 485, 586, 629, 879, 1113
AseI ATTAAT 1 cut(s) 1224
AspLEI GCGC 3 cut(s) 275, 474, 1396
AspS9I GGNCC 4 cut(s) 70, 245, 290, 1033
AsuHPI GGTGA 1 cut(s) 366
AsuII TTCGAA 2 cut(s) 215, 907
AvaII GGWCC 1 cut(s) 290
BbrPI CACGTG 2 cut(s) 116, 932
BbvI GCAGC 5 cut(s) 121, 388, 679, 1089, 1252
BccI CCATC 2 cut(s) 1346, 1375
BcgI CGANNNNNNTGC 2 cut(s) 799, 833
BciT130I CCWGG 3 cut(s) 105, 249, 1082
BcoDI GTCTC 5 cut(s) 207, 675, 737, 1063, 1253
BfaI CTAG 2 cut(s) 368, 1412
BfmI CTRYAG 1 cut(s) 609
BfoI RGCGCY 2 cut(s) 276, 475
BfuAI ACCTGC 1 cut(s) 1282
BglII AGATCT 1 cut(s) 170
BisI GCNGC 5 cut(s) 110, 402, 693, 1078, 1241
BlpI GCTNAGC 1 cut(s) 1044
BlsI GCNGC 5 cut(s) 111, 403, 694, 1079, 1242
BmcAI AGTACT 1 cut(s) 637
Bme1390I CCNGG 3 cut(s) 105, 249, 1082
Bme18I GGWCC 1 cut(s) 290
BmgT120I GGNCC 4 cut(s) 70, 245, 290, 1033
BmiI GGNNCC 2 cut(s) 617, 1197
BmrFI CCNGG 3 cut(s) 105, 249, 1082
BmsI GCATC 4 cut(s) 14, 679, 757, 967
Bpu1102I GCTNAGC 1 cut(s) 1044
Bpu14I TTCGAA 2 cut(s) 215, 907
BpuEI CTTGAG 3 cut(s) 581, 774, 1235
BsaAI YACGTR 2 cut(s) 116, 932
BsaI GGTCTC 3 cut(s) 675, 737, 1253
BsaJI CCNNGG 3 cut(s) 14, 78, 104
BsaXI ACNNNNNCTCC 2 cut(s) 644, 674
Bsc4I CCNNNNNNNGG 5 cut(s) 79, 308, 625, 1133, 1205
Bse118I RCCGGY 4 cut(s) 380, 536, 836, 941
Bse1I ACTGG 1 cut(s) 671
BseBI CCWGG 3 cut(s) 105, 249, 1082
BseDI CCNNGG 3 cut(s) 14, 78, 104
BseGI GGATG 1 cut(s) 24
BseLI CCNNNNNNNGG 5 cut(s) 79, 308, 625, 1133, 1205
BseMII CTCAG 2 cut(s) 23, 743
BseNI ACTGG 1 cut(s) 671
BseRI GAGGAG 1 cut(s) 1362
BseXI GCAGC 5 cut(s) 121, 388, 679, 1089, 1252
BseYI CCCAGC 1 cut(s) 1243
BsgI GTGCAG 3 cut(s) 81, 954, 1096
BshFI GGCC 3 cut(s) 71, 247, 1034
BsiSI CCGG 4 cut(s) 381, 537, 837, 942
BslFI GGGAC 1 cut(s) 464
BslI CCNNNNNNNGG 5 cut(s) 79, 308, 625, 1133, 1205
BsmAI GTCTC 5 cut(s) 207, 675, 737, 1063, 1253
BsmFI GGGAC 1 cut(s) 464
BsmI GAATGC 1 cut(s) 1155
BsnI GGCC 3 cut(s) 71, 247, 1034
Bso31I GGTCTC 3 cut(s) 675, 737, 1253
Bsp119I TTCGAA 2 cut(s) 215, 907
Bsp1407I TGTACA 1 cut(s) 1024
Bsp143I GATC 3 cut(s) 170, 1180, 1385
Bsp1720I GCTNAGC 1 cut(s) 1044
Bsp19I CCATGG 2 cut(s) 14, 78
BspACI CCGC 2 cut(s) 221, 650
BspANI GGCC 3 cut(s) 71, 247, 1034
BspCNI CTCAG 2 cut(s) 22, 742
BspLI GGNNCC 2 cut(s) 617, 1197
BspMI ACCTGC 1 cut(s) 1282
BspT104I TTCGAA 2 cut(s) 215, 907
BspTNI GGTCTC 3 cut(s) 675, 737, 1253
BsrFI RCCGGY 4 cut(s) 380, 536, 836, 941
BsrGI TGTACA 1 cut(s) 1024
BsrI ACTGG 1 cut(s) 671
BssAI RCCGGY 4 cut(s) 380, 536, 836, 941
BssECI CCNNGG 3 cut(s) 14, 78, 104
BssMI GATC 3 cut(s) 170, 1180, 1385
BssT1I CCWWGG 2 cut(s) 14, 78
Bst2UI CCWGG 3 cut(s) 105, 249, 1082
Bst4CI ACNGT 6 cut(s) 290, 338, 455, 725, 1000, 1261
BstAUI TGTACA 1 cut(s) 1024
BstBAI YACGTR 2 cut(s) 116, 932
BstBI TTCGAA 2 cut(s) 215, 907
BstC8I GCNNGC 4 cut(s) 139, 754, 943, 1431
BstDEI CTNAG 4 cut(s) 9, 729, 806, 1044
BstDSI CCRYGG 2 cut(s) 14, 78
BstF5I GGATG 1 cut(s) 24
BstH2I RGCGCY 2 cut(s) 276, 475
BstHHI GCGC 3 cut(s) 275, 474, 1396
BstKTI GATC 3 cut(s) 173, 1183, 1388
BstMAI GTCTC 5 cut(s) 207, 675, 737, 1063, 1253
BstMBI GATC 3 cut(s) 170, 1180, 1385
BstMWI GCNNNNNNNGC 3 cut(s) 68, 109, 1040
BstNI CCWGG 3 cut(s) 105, 249, 1082
BstNSI RCATGY 1 cut(s) 361
BstSCI CCNGG 3 cut(s) 103, 247, 1080
BstSFI CTRYAG 1 cut(s) 609
BstV1I GCAGC 5 cut(s) 121, 388, 679, 1089, 1252
BstX2I RGATCY 1 cut(s) 170
BstXI CCANNNNNNTGG 1 cut(s) 339
BstYI RGATCY 1 cut(s) 170
BsuRI GGCC 3 cut(s) 71, 247, 1034
BtgI CCRYGG 2 cut(s) 14, 78
BtsCI GGATG 1 cut(s) 24
BtsI GCAGTG 2 cut(s) 69, 1424
BtsIMutI CAGTG 4 cut(s) 69, 1005, 1197, 1424
BveI ACCTGC 1 cut(s) 1282
Cac8I GCNNGC 4 cut(s) 139, 754, 943, 1431
CaiI CAGNNNCTG 1 cut(s) 1087
CfoI GCGC 3 cut(s) 275, 474, 1396
Cfr10I RCCGGY 4 cut(s) 380, 536, 836, 941
Cfr13I GGNCC 4 cut(s) 70, 245, 290, 1033
Csp6I GTAC 3 cut(s) 364, 636, 1025
CviAII CATG 9 cut(s) 15, 43, 55, 79, 358, 674, 1040, 1436, 1440
CviQI GTAC 3 cut(s) 364, 636, 1025
DdeI CTNAG 4 cut(s) 9, 729, 806, 1044
DpnI GATC 3 cut(s) 172, 1182, 1387
DpnII GATC 3 cut(s) 170, 1180, 1385
DraI TTTAAA 2 cut(s) 298, 1011
DraIII CACNNNGTG 1 cut(s) 547
Eco130I CCWWGG 2 cut(s) 14, 78
Eco31I GGTCTC 3 cut(s) 675, 737, 1253
Eco32I GATATC 1 cut(s) 1324
Eco47I GGWCC 1 cut(s) 290
Eco47III AGCGCT 1 cut(s) 473
Eco57I CTGAAG 3 cut(s) 452, 696, 897
Eco72I CACGTG 2 cut(s) 116, 932
EcoO109I RGGNCCY 3 cut(s) 70, 245, 1033
EcoRI GAATTC 1 cut(s) 629
EcoRII CCWGG 3 cut(s) 103, 247, 1080
EcoRV GATATC 1 cut(s) 1324
EcoT14I CCWWGG 2 cut(s) 14, 78
EcoT22I ATGCAT 1 cut(s) 1441
ErhI CCWWGG 2 cut(s) 14, 78
FaeI CATG 9 cut(s) 18, 46, 58, 82, 361, 677, 1043, 1439, 1443
FalI AAGNNNNNCTT 2 cut(s) 1336, 1368
FaqI GGGAC 1 cut(s) 464
FatI CATG 9 cut(s) 14, 42, 54, 78, 357, 673, 1039, 1435, 1439
FblI GTMKAC 2 cut(s) 153, 819
Fnu4HI GCNGC 5 cut(s) 110, 402, 693, 1078, 1241
FokI GGATG 1 cut(s) 31
Fsp4HI GCNGC 5 cut(s) 110, 402, 693, 1078, 1241
FspBI CTAG 2 cut(s) 368, 1412
GlaI GCGC 3 cut(s) 274, 473, 1395
GluI GCNGC 5 cut(s) 110, 402, 693, 1078, 1241
GsaI CCCAGC 1 cut(s) 1247
HaeII RGCGCY 2 cut(s) 276, 475
HaeIII GGCC 3 cut(s) 71, 247, 1034
HapII CCGG 4 cut(s) 381, 537, 837, 942
HhaI GCGC 3 cut(s) 275, 474, 1396
Hin1II CATG 9 cut(s) 18, 46, 58, 82, 361, 677, 1043, 1439, 1443
Hin6I GCGC 3 cut(s) 273, 472, 1394
HinP1I GCGC 3 cut(s) 273, 472, 1394
HincII GTYRAC 2 cut(s) 820, 1257
HindII GTYRAC 2 cut(s) 820, 1257
HindIII AAGCTT 1 cut(s) 1046
HinfI GANTC 5 cut(s) 200, 328, 371, 1056, 1187
HpaII CCGG 4 cut(s) 381, 537, 837, 942
HphI GGTGA 1 cut(s) 366
Hpy166II GTNNAC 5 cut(s) 154, 820, 929, 1257, 1305
Hpy188I TCNGA 5 cut(s) 688, 766, 778, 885, 1208
Hpy188III TCNNGA 2 cut(s) 491, 1184
Hpy8I GTNNAC 5 cut(s) 154, 820, 929, 1257, 1305
Hpy99I CGWCG 1 cut(s) 262
HpyAV CCTTC 2 cut(s) 83, 1046
HpyCH4III ACNGT 6 cut(s) 290, 338, 455, 725, 1000, 1261
HpyCH4IV ACGT 4 cut(s) 115, 144, 831, 931
HpyCH4V TGCA 9 cut(s) 24, 62, 137, 560, 720, 935, 1077, 1090, 1439
HpyF10VI GCNNNNNNNGC 3 cut(s) 68, 109, 1040
HpyF3I CTNAG 4 cut(s) 9, 729, 806, 1044
HpySE526I ACGT 4 cut(s) 115, 144, 831, 931
Hsp92II CATG 9 cut(s) 18, 46, 58, 82, 361, 677, 1043, 1439, 1443
HspAI GCGC 3 cut(s) 273, 472, 1394
KroI GCCGGC 1 cut(s) 941
KroNI GCCGGC 1 cut(s) 943
Kzo9I GATC 3 cut(s) 170, 1180, 1385
LmnI GCTCC 2 cut(s) 1349, 1369
Lsp1109I GCAGC 5 cut(s) 121, 388, 679, 1089, 1252
LweI GCATC 4 cut(s) 14, 679, 757, 967
MaeI CTAG 2 cut(s) 368, 1412
MaeII ACGT 4 cut(s) 115, 144, 831, 931
MaeIII GTNAC 4 cut(s) 372, 539, 832, 1000
MalI GATC 3 cut(s) 172, 1182, 1387
MboI GATC 3 cut(s) 170, 1180, 1385
MboII GAAGA 2 cut(s) 458, 1004
MflI RGATCY 1 cut(s) 170
MlyI GAGTC 3 cut(s) 209, 380, 1065
MmeI TCCRAC 4 cut(s) 151, 244, 320, 890
Mph1103I ATGCAT 1 cut(s) 1441
MroNI GCCGGC 1 cut(s) 941
MseI TTAA 7 cut(s) 35, 297, 458, 893, 1010, 1224, 1401
MslI CAYNNNNRTG 1 cut(s) 19
MspA1I CMGCKG 1 cut(s) 1243
MspI CCGG 4 cut(s) 381, 537, 837, 942
MspR9I CCNGG 3 cut(s) 105, 249, 1082
Mva1269I GAATGC 1 cut(s) 1155
MvaI CCWGG 3 cut(s) 105, 249, 1082
MwoI GCNNNNNNNGC 3 cut(s) 68, 109, 1040
NaeI GCCGGC 1 cut(s) 943
NcoI CCATGG 2 cut(s) 14, 78
NdeII GATC 3 cut(s) 170, 1180, 1385
NgoMIV GCCGGC 1 cut(s) 941
NlaIII CATG 9 cut(s) 18, 46, 58, 82, 361, 677, 1043, 1439, 1443
NlaIV GGNNCC 2 cut(s) 617, 1197
NmuCI GTSAC 3 cut(s) 372, 539, 1000
NsiI ATGCAT 1 cut(s) 1441
NspI RCATGY 1 cut(s) 361
NspV TTCGAA 2 cut(s) 215, 907
PciI ACATGT 1 cut(s) 357
PcsI WCGNNNNNNNCGW 1 cut(s) 837
PctI GAATGC 1 cut(s) 1155
PdiI GCCGGC 1 cut(s) 943
PfeI GAWTC 2 cut(s) 328, 1187
PkrI GCNGC 5 cut(s) 111, 403, 694, 1079, 1242
PleI GAGTC 3 cut(s) 208, 379, 1064
PmaCI CACGTG 2 cut(s) 116, 932
PmlI CACGTG 2 cut(s) 116, 932
PpsI GAGTC 3 cut(s) 208, 379, 1064
Ppu21I YACGTR 2 cut(s) 116, 932
PscI ACATGT 1 cut(s) 357
PshBI ATTAAT 1 cut(s) 1224
PsiI TTATAA 1 cut(s) 951
Psp6I CCWGG 3 cut(s) 103, 247, 1080
PspCI CACGTG 2 cut(s) 116, 932
PspFI CCCAGC 1 cut(s) 1243
PspGI CCWGG 3 cut(s) 103, 247, 1080
PspN4I GGNNCC 2 cut(s) 617, 1197
PspPI GGNCC 4 cut(s) 70, 245, 290, 1033
PstNI CAGNNNCTG 1 cut(s) 1087
PsuI RGATCY 1 cut(s) 170
PvuII CAGCTG 1 cut(s) 1243
RsaI GTAC 3 cut(s) 365, 637, 1026
RsaNI GTAC 3 cut(s) 364, 636, 1025
RseI CAYNNNNRTG 1 cut(s) 19
SalI GTCGAC 1 cut(s) 818
SaqAI TTAA 7 cut(s) 35, 297, 458, 893, 1010, 1224, 1401
SatI GCNGC 5 cut(s) 110, 402, 693, 1078, 1241
Sau3AI GATC 3 cut(s) 170, 1180, 1385
Sau96I GGNCC 4 cut(s) 70, 245, 290, 1033
ScaI AGTACT 1 cut(s) 637
SchI GAGTC 3 cut(s) 209, 380, 1065
ScrFI CCNGG 3 cut(s) 105, 249, 1082
SfaNI GCATC 4 cut(s) 14, 679, 757, 967
SfcI CTRYAG 1 cut(s) 609
SfuI TTCGAA 2 cut(s) 215, 907
SinI GGWCC 1 cut(s) 290
SmiMI CAYNNNNRTG 1 cut(s) 19
SmlI CTYRAG 3 cut(s) 596, 789, 1250
SmoI CTYRAG 3 cut(s) 596, 789, 1250
SsiI CCGC 2 cut(s) 221, 650
SspMI CTAG 2 cut(s) 368, 1412
StyD4I CCNGG 3 cut(s) 103, 247, 1080
StyI CCWWGG 2 cut(s) 14, 78
TaaI ACNGT 6 cut(s) 290, 338, 455, 725, 1000, 1261
TaiI ACGT 4 cut(s) 118, 147, 834, 934
TaqI TCGA 4 cut(s) 215, 819, 907, 1213
TatI WGTACW 2 cut(s) 635, 1024
TfiI GAWTC 2 cut(s) 328, 1187
Tru1I TTAA 7 cut(s) 35, 297, 458, 893, 1010, 1224, 1401
Tru9I TTAA 7 cut(s) 35, 297, 458, 893, 1010, 1224, 1401
TscAI CASTG 4 cut(s) 69, 1005, 1197, 1431
TseFI GTSAC 3 cut(s) 372, 539, 1000
TseI GCWGC 5 cut(s) 109, 401, 692, 1077, 1240
Tsp45I GTSAC 3 cut(s) 372, 539, 1000
TspDTI ATGAA 2 cut(s) 911, 1028
TspGWI ACGGA 1 cut(s) 980
TspRI CASTG 4 cut(s) 69, 1005, 1197, 1431
VpaK11BI GGWCC 1 cut(s) 290
VspI ATTAAT 1 cut(s) 1224
XapI RAATTY 5 cut(s) 485, 586, 629, 879, 1113
XceI RCATGY 1 cut(s) 361
XmiI GTMKAC 2 cut(s) 153, 819
XspI CTAG 2 cut(s) 368, 1412
ZrmI AGTACT 1 cut(s) 637
Zsp2I ATGCAT 1 cut(s) 1441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.