Prupe.3G020300_v2.0.a1

lactosylceramide 4-alpha-galactosyltransferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
N/A
Physical Location & Seq
Reverse (-)
1484854 .. 1485728
875 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G020300.1_v2.0.a1.CDS.3

Sequence Viewer

Length: 528 bp
ATGCAATTTATCACCATCCAGCCACTCAAGTGTAGGAGAACTAACTTGACCAAAAGCGACTTGAAAACTGCTTCCCAATTATCTTTCACACTGGCACCCCCAGTCACAATTTCGTCACTGTTCCAACCACTTTCTTCTAGCAAGTTCCTGGTCCTCTGGTTCCTCCAGAAAATGTCACAAAAGGACAAGCTCCCGGAACTGCAGATACTCAACTCAAACAATTTAACAGAGCAGTTTCATGGTCGAGTACTTGAGTTCCTCAACCATGGATGCAGTGCTCAGTTCTACATGATATGGTTTTCACCAGCAACCCAGTTTGGCAAAAGACAAGTCATGGCCACGGACGGTCTCTTAAAATTCAAACCTAAAGGCTACTTGATGATCCTATCAAAAAGCATGGATTCAGGAAGTGGATACAGAATCCTGAAGCCGCTTCTCGATCGAGGATTCAAAGTAAAAGCATTAACACCAGACCTGCCCTTTCTAGTCAAGAACACCCCAGCTGAAACTTGGCTTCAAGAATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00601 ko00603 ko01100 map00601 map00603 map01100
Pfam Domains
Protein Families
Gb3_synth Gly_transf_sug

Protein Analysis

176

Amino Acids

19.98

Weight (kDa)

9.88

Isoelectric Point (pI)

43.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 483
AccB1I GGYRCC 1 cut(s) 94
AciI CCGC 1 cut(s) 431
AclWI GGATC 1 cut(s) 376
AcoI YGGCCR 1 cut(s) 336
AcsI RAATTY 1 cut(s) 356
AcuI CTGAAG 1 cut(s) 446
AfaI GTAC 1 cut(s) 249
AgsI TTSAA 4 cut(s) 64, 361, 451, 518
AjnI CCWGG 1 cut(s) 147
AleI CACNNNNGTG 1 cut(s) 28
AluBI AGCT 2 cut(s) 190, 503
AluI AGCT 2 cut(s) 190, 503
Alw21I GWGCWC 1 cut(s) 280
Alw26I GTCTC 1 cut(s) 353
AlwI GGATC 1 cut(s) 376
AoxI GGCC 1 cut(s) 336
ApoI RAATTY 1 cut(s) 356
ArsI GACNNNNNNTTYG 2 cut(s) 315, 347
AspS9I GGNCC 1 cut(s) 151
AsuC2I CCSGG 1 cut(s) 194
AsuHPI GGTGA 2 cut(s) 4, 294
AvaII GGWCC 1 cut(s) 151
BalI TGGCCA 1 cut(s) 338
BanI GGYRCC 1 cut(s) 94
Bbv12I GWGCWC 1 cut(s) 280
BccI CCATC 1 cut(s) 23
BciT130I CCWGG 1 cut(s) 149
BciVI GTATCC 1 cut(s) 407
BcnI CCSGG 1 cut(s) 194
BcoDI GTCTC 1 cut(s) 353
BfaI CTAG 2 cut(s) 138, 485
BfmI CTRYAG 1 cut(s) 200
BfuAI ACCTGC 1 cut(s) 483
BfuI GTATCC 1 cut(s) 407
BisI GCNGC 1 cut(s) 431
BlsI GCNGC 1 cut(s) 432
BmcAI AGTACT 1 cut(s) 249
Bme1390I CCNGG 2 cut(s) 149, 194
Bme18I GGWCC 1 cut(s) 151
BmgT120I GGNCC 1 cut(s) 151
BmiI GGNNCC 2 cut(s) 96, 161
BmrFI CCNGG 2 cut(s) 149, 194
BmrI ACTGGG 2 cut(s) 95, 307
BmsI GCATC 1 cut(s) 260
BmuI ACTGGG 2 cut(s) 95, 307
BpmI CTGGAG 1 cut(s) 149
BpuEI CTTGAG 2 cut(s) 11, 272
BpuMI CCSGG 1 cut(s) 194
BsaI GGTCTC 1 cut(s) 353
BsaJI CCNNGG 2 cut(s) 265, 339
Bse1I ACTGG 3 cut(s) 96, 101, 313
BseBI CCWGG 1 cut(s) 149
BseDI CCNNGG 2 cut(s) 265, 339
BseGI GGATG 2 cut(s) 15, 275
BseMII CTCAG 1 cut(s) 293
BseNI ACTGG 3 cut(s) 96, 101, 313
BseYI CCCAGC 1 cut(s) 499
Bsh1285I CGRYCG 1 cut(s) 442
BshFI GGCC 1 cut(s) 338
BshNI GGYRCC 1 cut(s) 94
BsiEI CGRYCG 1 cut(s) 442
BsiHKAI GWGCWC 1 cut(s) 280
BsiSI CCGG 1 cut(s) 194
BsmAI GTCTC 1 cut(s) 353
BsnI GGCC 1 cut(s) 338
Bso31I GGTCTC 1 cut(s) 353
Bsp1286I GDGCHC 1 cut(s) 280
Bsp143I GATC 2 cut(s) 381, 439
Bsp19I CCATGG 1 cut(s) 265
BspACI CCGC 1 cut(s) 431
BspANI GGCC 1 cut(s) 338
BspCNI CTCAG 1 cut(s) 292
BspLI GGNNCC 2 cut(s) 96, 161
BspMAI CTGCAG 1 cut(s) 204
BspMI ACCTGC 1 cut(s) 483
BspPI GGATC 1 cut(s) 376
BspT107I GGYRCC 1 cut(s) 94
BspTNI GGTCTC 1 cut(s) 353
BsrI ACTGG 3 cut(s) 96, 101, 313
BssECI CCNNGG 2 cut(s) 265, 339
BssMI GATC 2 cut(s) 381, 439
BssT1I CCWWGG 1 cut(s) 265
Bst2UI CCWGG 1 cut(s) 149
Bst4CI ACNGT 2 cut(s) 120, 347
BstDEI CTNAG 1 cut(s) 279
BstDSI CCRYGG 2 cut(s) 265, 339
BstF5I GGATG 2 cut(s) 15, 275
BstKTI GATC 2 cut(s) 384, 442
BstMAI GTCTC 1 cut(s) 353
BstMBI GATC 2 cut(s) 381, 439
BstMCI CGRYCG 1 cut(s) 442
BstNI CCWGG 1 cut(s) 149
BstSCI CCNGG 2 cut(s) 147, 192
BstSFI CTRYAG 1 cut(s) 200
BsuI GTATCC 1 cut(s) 407
BsuRI GGCC 1 cut(s) 338
BtgI CCRYGG 2 cut(s) 265, 339
BtsCI GGATG 2 cut(s) 15, 275
BtsI GCAGTG 1 cut(s) 280
BtsIMutI CAGTG 3 cut(s) 89, 116, 280
BveI ACCTGC 1 cut(s) 483
Cfr13I GGNCC 1 cut(s) 151
Csp6I GTAC 1 cut(s) 248
CviAII CATG 5 cut(s) 239, 266, 289, 334, 397
CviJI RGCY 7 cut(s) 22, 190, 338, 372, 430, 503, 514
CviKI_1 RGCY 7 cut(s) 22, 190, 338, 372, 430, 503, 514
CviQI GTAC 1 cut(s) 248
DdeI CTNAG 1 cut(s) 279
DpnI GATC 2 cut(s) 383, 441
DpnII GATC 2 cut(s) 381, 439
EaeI YGGCCR 1 cut(s) 336
Eco130I CCWWGG 1 cut(s) 265
Eco31I GGTCTC 1 cut(s) 353
Eco47I GGWCC 1 cut(s) 151
Eco57I CTGAAG 1 cut(s) 446
EcoRII CCWGG 1 cut(s) 147
EcoT14I CCWWGG 1 cut(s) 265
ErhI CCWWGG 1 cut(s) 265
FaeI CATG 5 cut(s) 242, 269, 292, 337, 400
FaiI YATR 7 cut(s) 240, 267, 290, 295, 335, 398, 526
FatI CATG 5 cut(s) 238, 265, 288, 333, 396
Fnu4HI GCNGC 1 cut(s) 431
FokI GGATG 2 cut(s) 2, 282
Fsp4HI GCNGC 1 cut(s) 431
FspBI CTAG 2 cut(s) 138, 485
GluI GCNGC 1 cut(s) 431
GsaI CCCAGC 1 cut(s) 503
GsuI CTGGAG 1 cut(s) 149
HaeIII GGCC 1 cut(s) 338
HapII CCGG 1 cut(s) 194
Hin1II CATG 5 cut(s) 242, 269, 292, 337, 400
HinfI GANTC 3 cut(s) 401, 420, 447
HpaII CCGG 1 cut(s) 194
HphI GGTGA 2 cut(s) 4, 294
Hpy188III TCNNGA 6 cut(s) 166, 405, 424, 437, 490, 518
HpyCH4III ACNGT 2 cut(s) 120, 347
HpyCH4V TGCA 3 cut(s) 4, 202, 273
HpyF3I CTNAG 1 cut(s) 279
Hsp92II CATG 5 cut(s) 242, 269, 292, 337, 400
Kzo9I GATC 2 cut(s) 381, 439
LmnI GCTCC 1 cut(s) 195
LweI GCATC 1 cut(s) 260
MaeI CTAG 2 cut(s) 138, 485
MaeIII GTNAC 3 cut(s) 103, 114, 174
MalI GATC 2 cut(s) 383, 441
MboI GATC 2 cut(s) 381, 439
MboII GAAGA 1 cut(s) 126
MhlI GDGCHC 1 cut(s) 280
MlsI TGGCCA 1 cut(s) 338
MluCI AATT 6 cut(s) 5, 77, 108, 220, 356, 521
MluNI TGGCCA 1 cut(s) 338
MmeI TCCRAC 1 cut(s) 148
MnlI CCTC 4 cut(s) 164, 173, 269, 437
Mox20I TGGCCA 1 cut(s) 338
MscI TGGCCA 1 cut(s) 338
MseI TTAA 3 cut(s) 224, 353, 464
MslI CAYNNNNRTG 1 cut(s) 28
Msp20I TGGCCA 1 cut(s) 338
MspA1I CMGCKG 1 cut(s) 503
MspI CCGG 1 cut(s) 194
MspR9I CCNGG 2 cut(s) 149, 194
MvaI CCWGG 1 cut(s) 149
NciI CCSGG 1 cut(s) 194
NcoI CCATGG 1 cut(s) 265
NdeII GATC 2 cut(s) 381, 439
NlaIII CATG 5 cut(s) 242, 269, 292, 337, 400
NlaIV GGNNCC 2 cut(s) 96, 161
NmuCI GTSAC 3 cut(s) 103, 114, 174
OliI CACNNNNGTG 1 cut(s) 28
PfeI GAWTC 3 cut(s) 401, 420, 447
PfoI TCCNGGA 1 cut(s) 192
PkrI GCNGC 1 cut(s) 432
Ple19I CGATCG 1 cut(s) 442
Psp6I CCWGG 1 cut(s) 147
PspFI CCCAGC 1 cut(s) 499
PspGI CCWGG 1 cut(s) 147
PspN4I GGNNCC 2 cut(s) 96, 161
PspPI GGNCC 1 cut(s) 151
PsrI GAACNNNNNNTAC 2 cut(s) 189, 221
PstI CTGCAG 1 cut(s) 204
PvuI CGATCG 1 cut(s) 442
PvuII CAGCTG 1 cut(s) 503
RsaI GTAC 1 cut(s) 249
RsaNI GTAC 1 cut(s) 248
RseI CAYNNNNRTG 1 cut(s) 28
SaqAI TTAA 3 cut(s) 224, 353, 464
SatI GCNGC 1 cut(s) 431
Sau3AI GATC 2 cut(s) 381, 439
Sau96I GGNCC 1 cut(s) 151
ScaI AGTACT 1 cut(s) 249
ScrFI CCNGG 2 cut(s) 149, 194
SduI GDGCHC 1 cut(s) 280
SetI ASST 4 cut(s) 192, 367, 477, 505
SfaNI GCATC 1 cut(s) 260
SfcI CTRYAG 1 cut(s) 200
SinI GGWCC 1 cut(s) 151
SmiMI CAYNNNNRTG 1 cut(s) 28
SmlI CTYRAG 2 cut(s) 26, 251
SmoI CTYRAG 2 cut(s) 26, 251
Sse9I AATT 6 cut(s) 5, 77, 108, 220, 356, 521
SsiI CCGC 1 cut(s) 431
SspMI CTAG 2 cut(s) 138, 485
StyD4I CCNGG 2 cut(s) 147, 192
StyI CCWWGG 1 cut(s) 265
TaaI ACNGT 2 cut(s) 120, 347
TaqI TCGA 3 cut(s) 244, 438, 442
TasI AATT 6 cut(s) 5, 77, 108, 220, 356, 521
TatI WGTACW 1 cut(s) 247
TauI GCSGC 1 cut(s) 433
TfiI GAWTC 3 cut(s) 401, 420, 447
Tru1I TTAA 3 cut(s) 224, 353, 464
Tru9I TTAA 3 cut(s) 224, 353, 464
TscAI CASTG 3 cut(s) 96, 123, 280
TseFI GTSAC 3 cut(s) 103, 114, 174
Tsp45I GTSAC 3 cut(s) 103, 114, 174
TspDTI ATGAA 1 cut(s) 227
TspGWI ACGGA 1 cut(s) 356
TspRI CASTG 3 cut(s) 96, 123, 280
VpaK11BI GGWCC 1 cut(s) 151
XapI RAATTY 1 cut(s) 356
XcmI CCANNNNNNNNNTGG 1 cut(s) 507
XspI CTAG 2 cut(s) 138, 485
ZrmI AGTACT 1 cut(s) 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.