Prupe.3G042800_v2.0.a1

40S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
N/A
Physical Location & Seq
Forward (+)
3046741 .. 3051672
4932 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G042800.1_v2.0.a1.CDS.5

Sequence Viewer

Length: 555 bp
ATGGCTACTCAAACGAGCAAAAAGCGAAAGTTTGTAGCCAACGGAGTGTTTTTGGCGGAGCTGAACGAGGTGATGACCAGAGAGCTCGCAGAAGATGGGTACTCTGGCGTTGAAGTTAGGGTTACGCCAGTGCGGACCGAGATCATTATTAGAGCCATCCAGACTCAAAACGTCCTCGGTGAGAAGGGAAGAAGAATTAGAGAACTGACATCTGTTGTTCAGAAGCGGCTTTGTGCCATTGCTCAAGCAGAGTCTTTGCGTTACAAACTTCTTGGAGGGCTTGCTGTTCGGAGGGCATGCTATGGTGTTCTTAGATTTGTCATGGAGAGTGTAGCAAAGGAATGTGAGGTCATTGTCAGTGGCAAACTCAGGGCTCAGCCGCTGGGTGTTCTTGAGATGAAGGTCAAAATCATGCTCGATTGGGATCCCAAGGGTAAGCAAGGCCCCACAACGCCCCTACCGGATCTGGTCACCATCCACCAACCCAAAGAGGAAGAGGACTACATCAGGCCCTCAGTTGTGGCAGCAGCTGAAATAATAGTCCTTGCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
KH_2 Ribosomal_S3_C

Protein Analysis

185

Amino Acids

20.41

Weight (kDa)

9.32

Isoelectric Point (pI)

38.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 56, 133, 226, 380
AclWI GGATC 3 cut(s) 419, 432, 471
AfaI GTAC 1 cut(s) 101
AgsI TTSAA 1 cut(s) 113
AluBI AGCT 3 cut(s) 61, 85, 530
AluI AGCT 3 cut(s) 61, 85, 530
Alw21I GWGCWC 1 cut(s) 87
AlwI GGATC 3 cut(s) 419, 432, 471
AlwNI CAGNNNCTG 2 cut(s) 382, 530
AoxI GGCC 2 cut(s) 442, 509
ApeKI GCWGC 3 cut(s) 524, 527, 548
AspS9I GGNCC 3 cut(s) 135, 443, 510
AsuHPI GGTGA 3 cut(s) 82, 191, 463
AvaII GGWCC 1 cut(s) 135
BamHI GGATCC 1 cut(s) 424
BanII GRGCYC 2 cut(s) 87, 376
Bbv12I GWGCWC 1 cut(s) 87
BbvI GCAGC 3 cut(s) 535, 536, 539
BccI CCATC 3 cut(s) 89, 164, 482
BisI GCNGC 5 cut(s) 227, 380, 525, 528, 549
BlpI GCTNAGC 1 cut(s) 375
BlsI GCNGC 5 cut(s) 228, 381, 526, 529, 550
Bme18I GGWCC 1 cut(s) 135
BmgT120I GGNCC 3 cut(s) 135, 443, 510
BmiI GGNNCC 2 cut(s) 426, 445
Bpu1102I GCTNAGC 1 cut(s) 375
BpuEI CTTGAG 2 cut(s) 228, 413
BsaBI GATNNNNATC 1 cut(s) 423
BsaJI CCNNGG 2 cut(s) 175, 429
BsaWI WCCGGW 1 cut(s) 460
Bse1I ACTGG 1 cut(s) 128
Bse3DI GCAATG 1 cut(s) 237
Bse8I GATNNNNATC 1 cut(s) 423
BseDI CCNNGG 2 cut(s) 175, 429
BseGI GGATG 2 cut(s) 156, 474
BseJI GATNNNNATC 1 cut(s) 423
BseMI GCAATG 1 cut(s) 237
BseMII CTCAG 3 cut(s) 382, 389, 528
BseNI ACTGG 1 cut(s) 128
BseXI GCAGC 3 cut(s) 535, 536, 539
BseYI CCCAGC 1 cut(s) 382
BshFI GGCC 2 cut(s) 444, 511
BsiHKAI GWGCWC 1 cut(s) 87
BsiSI CCGG 1 cut(s) 461
BsnI GGCC 2 cut(s) 444, 511
Bsp1286I GDGCHC 2 cut(s) 87, 376
Bsp143I GATC 3 cut(s) 141, 424, 463
Bsp1720I GCTNAGC 1 cut(s) 375
BspACI CCGC 4 cut(s) 56, 133, 226, 380
BspANI GGCC 2 cut(s) 444, 511
BspCNI CTCAG 3 cut(s) 381, 388, 527
BspLI GGNNCC 2 cut(s) 426, 445
BspPI GGATC 3 cut(s) 419, 432, 471
BsrDI GCAATG 1 cut(s) 237
BsrI ACTGG 1 cut(s) 128
BssECI CCNNGG 2 cut(s) 175, 429
BssMI GATC 3 cut(s) 141, 424, 463
BssT1I CCWWGG 1 cut(s) 429
Bst6I CTCTTC 1 cut(s) 489
BstC8I GCNNGC 3 cut(s) 87, 282, 298
BstDEI CTNAG 5 cut(s) 311, 368, 375, 514, 552
BstEII GGTNACC 1 cut(s) 469
BstF5I GGATG 2 cut(s) 156, 474
BstKTI GATC 3 cut(s) 144, 427, 466
BstMBI GATC 3 cut(s) 141, 424, 463
BstNSI RCATGY 1 cut(s) 300
BstPI GGTNACC 1 cut(s) 469
BstV1I GCAGC 3 cut(s) 535, 536, 539
BstX2I RGATCY 2 cut(s) 424, 463
BstYI RGATCY 2 cut(s) 424, 463
BsuRI GGCC 2 cut(s) 444, 511
BtsCI GGATG 2 cut(s) 156, 474
BtsIMutI CAGTG 2 cut(s) 135, 364
Cac8I GCNNGC 3 cut(s) 87, 282, 298
CaiI CAGNNNCTG 2 cut(s) 382, 530
Cfr13I GGNCC 3 cut(s) 135, 443, 510
CpoI CGGWCCG 1 cut(s) 135
Csp6I GTAC 1 cut(s) 100
CspI CGGWCCG 1 cut(s) 135
CviAII CATG 3 cut(s) 297, 322, 412
CviQI GTAC 1 cut(s) 100
DdeI CTNAG 5 cut(s) 311, 368, 375, 514, 552
DpnI GATC 3 cut(s) 143, 426, 465
DpnII GATC 3 cut(s) 141, 424, 463
Eam1104I CTCTTC 1 cut(s) 489
EarI CTCTTC 1 cut(s) 489
EciI GGCGGA 1 cut(s) 71
Ecl136II GAGCTC 1 cut(s) 85
Eco130I CCWWGG 1 cut(s) 429
Eco24I GRGCYC 2 cut(s) 87, 376
Eco47I GGWCC 1 cut(s) 135
Eco53kI GAGCTC 1 cut(s) 85
Eco91I GGTNACC 1 cut(s) 469
EcoICRI GAGCTC 1 cut(s) 85
EcoO109I RGGNCCY 2 cut(s) 443, 510
EcoO65I GGTNACC 1 cut(s) 469
EcoT14I CCWWGG 1 cut(s) 429
EcoT38I GRGCYC 2 cut(s) 87, 376
ErhI CCWWGG 1 cut(s) 429
FaeI CATG 3 cut(s) 300, 325, 415
FaiI YATR 4 cut(s) 298, 303, 323, 413
FatI CATG 3 cut(s) 296, 321, 411
Fnu4HI GCNGC 5 cut(s) 227, 380, 525, 528, 549
FokI GGATG 2 cut(s) 143, 461
FriOI GRGCYC 2 cut(s) 87, 376
Fsp4HI GCNGC 5 cut(s) 227, 380, 525, 528, 549
GluI GCNGC 5 cut(s) 227, 380, 525, 528, 549
GsaI CCCAGC 1 cut(s) 386
HaeIII GGCC 2 cut(s) 444, 511
HapII CCGG 1 cut(s) 461
Hin1II CATG 3 cut(s) 300, 325, 415
HinfI GANTC 2 cut(s) 163, 251
HpaII CCGG 1 cut(s) 461
HphI GGTGA 3 cut(s) 82, 191, 463
Hpy188I TCNGA 2 cut(s) 222, 291
Hpy188III TCNNGA 2 cut(s) 160, 392
HpyAV CCTTC 2 cut(s) 178, 394
HpyCH4IV ACGT 1 cut(s) 171
HpyF3I CTNAG 5 cut(s) 311, 368, 375, 514, 552
HpySE526I ACGT 1 cut(s) 171
Hsp92II CATG 3 cut(s) 300, 325, 415
Kzo9I GATC 3 cut(s) 141, 424, 463
LmnI GCTCC 1 cut(s) 58
LpnPI CCDG 9 cut(s) 90, 91, 141, 173, 355, 368, 452, 474, 493
Lsp1109I GCAGC 3 cut(s) 535, 536, 539
MaeII ACGT 1 cut(s) 171
MaeIII GTNAC 3 cut(s) 121, 260, 469
MalI GATC 3 cut(s) 143, 426, 465
MboI GATC 3 cut(s) 141, 424, 463
MboII GAAGA 4 cut(s) 104, 201, 204, 506
MflI RGATCY 2 cut(s) 424, 463
MhlI GDGCHC 2 cut(s) 87, 376
MluCI AATT 1 cut(s) 195
MlyI GAGTC 2 cut(s) 157, 260
MnlI CCTC 8 cut(s) 61, 185, 269, 285, 340, 484, 490, 523
MspA1I CMGCKG 2 cut(s) 382, 530
MspI CCGG 1 cut(s) 461
NdeII GATC 3 cut(s) 141, 424, 463
NlaIII CATG 3 cut(s) 300, 325, 415
NlaIV GGNNCC 2 cut(s) 426, 445
NmuCI GTSAC 1 cut(s) 469
NspI RCATGY 1 cut(s) 300
PaeI GCATGC 1 cut(s) 300
PkrI GCNGC 5 cut(s) 228, 381, 526, 529, 550
PleI GAGTC 2 cut(s) 157, 259
PpsI GAGTC 2 cut(s) 157, 259
Psp124BI GAGCTC 1 cut(s) 87
PspEI GGTNACC 1 cut(s) 469
PspFI CCCAGC 1 cut(s) 382
PspN4I GGNNCC 2 cut(s) 426, 445
PspPI GGNCC 3 cut(s) 135, 443, 510
PstNI CAGNNNCTG 2 cut(s) 382, 530
PsuI RGATCY 2 cut(s) 424, 463
PvuII CAGCTG 1 cut(s) 530
RsaI GTAC 1 cut(s) 101
RsaNI GTAC 1 cut(s) 100
Rsr2I CGGWCCG 1 cut(s) 135
RsrII CGGWCCG 1 cut(s) 135
SacI GAGCTC 1 cut(s) 87
SatI GCNGC 5 cut(s) 227, 380, 525, 528, 549
Sau3AI GATC 3 cut(s) 141, 424, 463
Sau96I GGNCC 3 cut(s) 135, 443, 510
SchI GAGTC 2 cut(s) 157, 260
SduI GDGCHC 2 cut(s) 87, 376
SetI ASST 7 cut(s) 63, 72, 87, 174, 351, 405, 532
SinI GGWCC 1 cut(s) 135
SmlI CTYRAG 2 cut(s) 243, 392
SmoI CTYRAG 2 cut(s) 243, 392
SphI GCATGC 1 cut(s) 300
Sse9I AATT 1 cut(s) 195
SsiI CCGC 4 cut(s) 56, 133, 226, 380
SstI GAGCTC 1 cut(s) 87
StyI CCWWGG 1 cut(s) 429
TaiI ACGT 1 cut(s) 174
TaqI TCGA 1 cut(s) 417
TaqII GACCGA 1 cut(s) 152
TasI AATT 1 cut(s) 195
TauI GCSGC 2 cut(s) 229, 382
TscAI CASTG 2 cut(s) 135, 364
TseFI GTSAC 1 cut(s) 469
TseI GCWGC 3 cut(s) 524, 527, 548
Tsp45I GTSAC 1 cut(s) 469
TspDTI ATGAA 1 cut(s) 413
TspGWI ACGGA 1 cut(s) 57
TspRI CASTG 2 cut(s) 135, 364
VpaK11BI GGWCC 1 cut(s) 135
XceI RCATGY 1 cut(s) 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.