Prupe.3G058100_v2.0.a1

negative regulation of Rho guanyl-nucleotide exchange factor activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
N/A
Physical Location & Seq
Reverse (-)
4114428 .. 4116134
1707 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G058100.1_v2.0.a1.CDS.1

Sequence Viewer

Length: 1398 bp
ATGATGAATATTATGGTCCTTTTGCTAACAGCAGCAGCAAGCCTAGGGTTTGTAGTTGAAGCTCAAATAGGCCTTCCACCTCCTCCTATGCCTGGCCTTCCTCCTCCTATCATCCCACCAGGCTTTCCAAACCCTAACATTCCTGGTCTTTTCCCTCCCATCACTCCACCAGGCCTTCCTGACCCTTATCTTCCCGGTCTTACTCCACCTGGCTTTCCTAACCCTTATCTTCCCGGTCTCACTCCACCAGCCTTTCCTAACCCTAATCTTCCCAGTCTTACTCCACCAGCCTTTCCTAACCCTAACCTTCCCGGTCTCACTCCACCAGCCTTTCCTAACCCATTAATCCCTGGCCTCTCTCCTCCAACTACTCCTAGTGCACCAGTTCCAAGCCCTCCTCCTTATTTTAATCCTTTGGATCCAAACATTCCACTCCCTCCATATAGTCCTCAACCATCACCTTCTGTAGAAGCTCCTGCCAACAGTCCTCAACCATCAACTTTTACACTCCCACCAGAACTTTATAAAAACATAGGAAAAGCACCACCCTTTGTTATAGCACCCAATACTCCTCTACCACCGGGTGTTGTTATACCACCACCCCCACCCCTAAACACTCCACCGCCACCTGGTTGGTCTGCTGAAAAGATCGCCCTTAAAACCAGCATTTGTGGTGTTTCCTGCGCTTACGAATGCCAAGCCCCCGCCGTCTGGAGCACTCCCACCGCAAAAAATACCAATGGGTTATTCCGTGGACGCGGCGGCGGTGGCGGAGGCGGAGGCTTTTTCCGTCCAGTCCGTCCTGTCCGTCCTGTTACTCCCGTCCCCGTTCCCGTTACTCCTGTCCCCGTTATCCCTTCACCCGTTATCCGCCCTCCCCCCATCCCTACTCCAACCAAACCAAATCCACCCTCACCGCCTAGTCCCGTTGCTCCAGCCAAACCGAATCCCCCATCCACTCCTCCCACCGGTCCATCTCCACCCTCACCGCCTAGTCCCGCTGCTCCAGCCAAACCGAATCCCCCACCCTCTCCTCCCACCGGTCCATCTCCACCCGGTTCTCCCACCGGTCCATCTCCACCCGGTGCTCCTGCTGGTCCATCTCCACCTGGTTCTCCGGCTAGTCCAGCCAAACCCGGTCCTCCGGCTAGTCCATCGCCACCCGGTTCTCCCTCCGCTCCATCTCCACCTGGTTCTCCCGCCGCTCCATCTTCACCAGGTACTCCTGTCACACCAGTGACACCTGCGAGTCCCGTGGATCCAGTTCATCCTGTGGACCCCAATCACCCCGTACCCGGTTACCGTTACTCAAGAAAACGGGGTATGAATCAGGCAGGTTGTGTCGCTGCGTGTATGCTTCAATGCAACCAGGATTATTTACTTAGTCTCGTTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PL48 WSC

Protein Analysis

466

Amino Acids

46.66

Weight (kDa)

9.39

Isoelectric Point (pI)

94.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 525
AarI CACCTGC 1 cut(s) 1252
Acc36I ACCTGC 2 cut(s) 1252, 1325
AccBSI CCGCTC 2 cut(s) 1178, 1205
AccII CGCG 1 cut(s) 759
AclWI GGATC 4 cut(s) 413, 426, 1253, 1266
AdeI CACNNNGTG 2 cut(s) 584, 1085
AfaI GTAC 2 cut(s) 1222, 1293
AfiI CCNNNNNNNGG 6 cut(s) 92, 629, 711, 968, 1040, 1295
AgeI ACCGGT 3 cut(s) 968, 1040, 1067
AgsI TTSAA 2 cut(s) 59, 1361
AjuI GAANNNNNNNTTGG 2 cut(s) 731, 763
AleI CACNNNNGTG 1 cut(s) 1235
AluBI AGCT 2 cut(s) 62, 473
AluI AGCT 2 cut(s) 62, 473
Alw21I GWGCWC 3 cut(s) 382, 719, 1090
Alw26I GTCTC 3 cut(s) 242, 320, 1391
Alw44I GTGCAC 1 cut(s) 378
AlwI GGATC 4 cut(s) 413, 426, 1253, 1266
AoxI GGCC 4 cut(s) 70, 94, 172, 352
ApaLI GTGCAC 1 cut(s) 378
ApeKI GCWGC 4 cut(s) 32, 35, 1001, 1346
AseI ATTAAT 1 cut(s) 344
AsiGI ACCGGT 3 cut(s) 968, 1040, 1067
AspA2I CCTAGG 1 cut(s) 43
AspLEI GCGC 1 cut(s) 686
AspS9I GGNCC 7 cut(s) 16, 971, 1043, 1070, 1097, 1139, 1276
AsuC2I CCSGG 9 cut(s) 195, 234, 312, 582, 1056, 1083, 1137, 1164, 1296
AsuHPI GGTGA 6 cut(s) 450, 852, 906, 978, 1206, 1277
AvaII GGWCC 7 cut(s) 16, 971, 1043, 1070, 1097, 1139, 1276
AvrII CCTAGG 1 cut(s) 43
BaeGI GKGCMC 1 cut(s) 382
BaeI ACNNNNGTAYC 2 cut(s) 1212, 1245
BamHI GGATCC 2 cut(s) 418, 1258
Bbv12I GWGCWC 3 cut(s) 382, 719, 1090
BbvI GCAGC 4 cut(s) 44, 47, 988, 1333
BceAI ACGGC 1 cut(s) 692
BcnI CCSGG 9 cut(s) 195, 234, 312, 582, 1056, 1083, 1137, 1164, 1296
BcoDI GTCTC 3 cut(s) 242, 320, 1391
BfaI CTAG 6 cut(s) 44, 375, 921, 993, 1122, 1149
BfmI CTRYAG 1 cut(s) 465
BfuAI ACCTGC 2 cut(s) 1252, 1325
BisI GCNGC 7 cut(s) 33, 36, 760, 763, 1002, 1203, 1347
BlnI CCTAGG 1 cut(s) 43
BlsI GCNGC 7 cut(s) 34, 37, 761, 764, 1003, 1204, 1348
Bme18I GGWCC 7 cut(s) 16, 971, 1043, 1070, 1097, 1139, 1276
BmgT120I GGNCC 7 cut(s) 16, 971, 1043, 1070, 1097, 1139, 1276
BmiI GGNNCC 3 cut(s) 420, 1260, 1278
BmrI ACTGGG 1 cut(s) 267
BmuI ACTGGG 1 cut(s) 267
BpmI CTGGAG 3 cut(s) 733, 918, 990
BpuEI CTTGAG 1 cut(s) 1294
BpuMI CCSGG 9 cut(s) 195, 234, 312, 582, 1056, 1083, 1137, 1164, 1296
BsaI GGTCTC 2 cut(s) 242, 320
BsaJI CCNNGG 4 cut(s) 43, 349, 751, 1254
BsaWI WCCGGW 3 cut(s) 968, 1040, 1067
Bsc4I CCNNNNNNNGG 6 cut(s) 92, 629, 711, 968, 1040, 1295
Bse118I RCCGGY 3 cut(s) 968, 1040, 1067
Bse1I ACTGG 5 cut(s) 273, 383, 794, 1235, 1262
BseDI CCNNGG 4 cut(s) 43, 349, 751, 1254
BseGI GGATG 4 cut(s) 111, 882, 953, 1267
BseLI CCNNNNNNNGG 6 cut(s) 92, 629, 711, 968, 1040, 1295
BseNI ACTGG 5 cut(s) 273, 383, 794, 1235, 1262
BseRI GAGGAG 7 cut(s) 72, 93, 351, 387, 561, 951, 1023
BseSI GKGCMC 1 cut(s) 382
BseXI GCAGC 4 cut(s) 44, 47, 988, 1333
Bsh1236I CGCG 1 cut(s) 759
BshFI GGCC 4 cut(s) 72, 96, 174, 354
BshTI ACCGGT 3 cut(s) 968, 1040, 1067
BsiHKAI GWGCWC 3 cut(s) 382, 719, 1090
BslFI GGGAC 5 cut(s) 809, 830, 909, 981, 1236
BslI CCNNNNNNNGG 6 cut(s) 92, 629, 711, 968, 1040, 1295
BsmAI GTCTC 3 cut(s) 242, 320, 1391
BsmFI GGGAC 5 cut(s) 809, 830, 909, 981, 1236
BsmI GAATGC 1 cut(s) 698
BsnI GGCC 4 cut(s) 72, 96, 174, 354
Bso31I GGTCTC 2 cut(s) 242, 320
Bsp1286I GDGCHC 3 cut(s) 382, 719, 1090
Bsp143I GATC 3 cut(s) 418, 648, 1258
BspANI GGCC 4 cut(s) 72, 96, 174, 354
BspFNI CGCG 1 cut(s) 759
BspLI GGNNCC 3 cut(s) 420, 1260, 1278
BspMI ACCTGC 2 cut(s) 1252, 1325
BspPI GGATC 4 cut(s) 413, 426, 1253, 1266
BspTNI GGTCTC 2 cut(s) 242, 320
BsrBI CCGCTC 2 cut(s) 1178, 1205
BsrFI RCCGGY 3 cut(s) 968, 1040, 1067
BsrI ACTGG 5 cut(s) 273, 383, 794, 1235, 1262
BssAI RCCGGY 3 cut(s) 968, 1040, 1067
BssECI CCNNGG 4 cut(s) 43, 349, 751, 1254
BssMI GATC 3 cut(s) 418, 648, 1258
BssT1I CCWWGG 1 cut(s) 43
Bst4CI ACNGT 2 cut(s) 485, 1304
BstC8I GCNNGC 1 cut(s) 40
BstDEI CTNAG 1 cut(s) 1382
BstDSI CCRYGG 2 cut(s) 751, 1254
BstEII GGTNACC 1 cut(s) 1298
BstF5I GGATG 4 cut(s) 111, 882, 953, 1267
BstFNI CGCG 1 cut(s) 759
BstHHI GCGC 1 cut(s) 686
BstKTI GATC 3 cut(s) 421, 651, 1261
BstMAI GTCTC 3 cut(s) 242, 320, 1391
BstMBI GATC 3 cut(s) 418, 648, 1258
BstMWI GCNNNNNNNGC 3 cut(s) 768, 1007, 1127
BstPI GGTNACC 1 cut(s) 1298
BstSFI CTRYAG 1 cut(s) 465
BstSLI GKGCMC 1 cut(s) 382
BstUI CGCG 1 cut(s) 759
BstV1I GCAGC 4 cut(s) 44, 47, 988, 1333
BstX2I RGATCY 2 cut(s) 418, 1258
BstXI CCANNNNNNTGG 1 cut(s) 633
BstYI RGATCY 2 cut(s) 418, 1258
BsuRI GGCC 4 cut(s) 72, 96, 174, 354
BtgI CCRYGG 2 cut(s) 751, 1254
BtgZI GCGATG 1 cut(s) 1140
BtsCI GGATG 4 cut(s) 111, 882, 953, 1267
BtsIMutI CAGTG 1 cut(s) 1242
BveI ACCTGC 2 cut(s) 1252, 1325
Cac8I GCNNGC 1 cut(s) 40
CfoI GCGC 1 cut(s) 686
Cfr10I RCCGGY 3 cut(s) 968, 1040, 1067
Cfr13I GGNCC 7 cut(s) 16, 971, 1043, 1070, 1097, 1139, 1276
CseI GACGC 1 cut(s) 765
CsiI ACCWGGT 4 cut(s) 628, 1108, 1189, 1216
Csp6I GTAC 2 cut(s) 1221, 1292
CspAI ACCGGT 3 cut(s) 968, 1040, 1067
CviQI GTAC 2 cut(s) 1221, 1292
DdeI CTNAG 1 cut(s) 1382
DpnI GATC 3 cut(s) 420, 650, 1260
DpnII GATC 3 cut(s) 418, 648, 1258
DraIII CACNNNGTG 2 cut(s) 584, 1085
EciI GGCGGA 3 cut(s) 786, 792, 860
Eco130I CCWWGG 1 cut(s) 43
Eco147I AGGCCT 2 cut(s) 72, 174
Eco31I GGTCTC 2 cut(s) 242, 320
Eco47I GGWCC 7 cut(s) 16, 971, 1043, 1070, 1097, 1139, 1276
Eco91I GGTNACC 1 cut(s) 1298
EcoO65I GGTNACC 1 cut(s) 1298
EcoT14I CCWWGG 1 cut(s) 43
ErhI CCWWGG 1 cut(s) 43
FaqI GGGAC 5 cut(s) 809, 830, 909, 981, 1236
FauI CCCGC 3 cut(s) 712, 1006, 1207
Fnu4HI GCNGC 7 cut(s) 33, 36, 760, 763, 1002, 1203, 1347
FokI GGATG 4 cut(s) 98, 869, 940, 1254
Fsp4HI GCNGC 7 cut(s) 33, 36, 760, 763, 1002, 1203, 1347
FspBI CTAG 6 cut(s) 44, 375, 921, 993, 1122, 1149
GlaI GCGC 1 cut(s) 685
GluI GCNGC 7 cut(s) 33, 36, 760, 763, 1002, 1203, 1347
GsuI CTGGAG 3 cut(s) 733, 918, 990
HaeIII GGCC 4 cut(s) 72, 96, 174, 354
HgaI GACGC 1 cut(s) 765
HhaI GCGC 1 cut(s) 686
Hin6I GCGC 1 cut(s) 684
HinP1I GCGC 1 cut(s) 684
HinfI GANTC 4 cut(s) 946, 1018, 1249, 1327
HphI GGTGA 6 cut(s) 450, 852, 906, 978, 1206, 1277
Hpy166II GTNNAC 3 cut(s) 380, 755, 1276
Hpy188III TCNNGA 3 cut(s) 179, 712, 1311
Hpy8I GTNNAC 3 cut(s) 380, 755, 1276
HpyAV CCTTC 6 cut(s) 83, 107, 185, 317, 471, 867
HpyCH4III ACNGT 2 cut(s) 485, 1304
HpyCH4V TGCA 2 cut(s) 380, 1365
HpyF10VI GCNNNNNNNGC 3 cut(s) 768, 1007, 1127
HpyF3I CTNAG 1 cut(s) 1382
HspAI GCGC 1 cut(s) 684
Kzo9I GATC 3 cut(s) 418, 648, 1258
LmnI GCTCC 7 cut(s) 478, 714, 937, 1009, 1093, 1183, 1210
Lsp1109I GCAGC 4 cut(s) 44, 47, 988, 1333
MabI ACCWGGT 4 cut(s) 628, 1108, 1189, 1216
MaeI CTAG 6 cut(s) 44, 375, 921, 993, 1122, 1149
MaeIII GTNAC 6 cut(s) 814, 835, 1228, 1237, 1298, 1304
MalI GATC 3 cut(s) 420, 650, 1260
MbiI CCGCTC 2 cut(s) 1178, 1205
MboI GATC 3 cut(s) 418, 648, 1258
MboII GAAGA 4 cut(s) 182, 221, 260, 1203
MflI RGATCY 2 cut(s) 418, 1258
MhlI GDGCHC 3 cut(s) 382, 719, 1090
MlyI GAGTC 1 cut(s) 1258
MmeI TCCRAC 2 cut(s) 389, 917
MseI TTAA 3 cut(s) 344, 408, 657
MslI CAYNNNNRTG 1 cut(s) 1235
MspA1I CMGCKG 1 cut(s) 1001
Mva1269I GAATGC 1 cut(s) 698
MvnI CGCG 1 cut(s) 759
MwoI GCNNNNNNNGC 3 cut(s) 768, 1007, 1127
NciI CCSGG 9 cut(s) 195, 234, 312, 582, 1056, 1083, 1137, 1164, 1296
NdeII GATC 3 cut(s) 418, 648, 1258
NlaIV GGNNCC 3 cut(s) 420, 1260, 1278
NmuCI GTSAC 2 cut(s) 1228, 1237
OliI CACNNNNGTG 1 cut(s) 1235
PaqCI CACCTGC 1 cut(s) 1252
PceI AGGCCT 2 cut(s) 72, 174
PctI GAATGC 1 cut(s) 698
PfeI GAWTC 3 cut(s) 946, 1018, 1327
PinAI ACCGGT 3 cut(s) 968, 1040, 1067
PkrI GCNGC 7 cut(s) 34, 37, 761, 764, 1003, 1204, 1348
PleI GAGTC 1 cut(s) 1257
PpsI GAGTC 1 cut(s) 1257
PshBI ATTAAT 1 cut(s) 344
PsiI TTATAA 1 cut(s) 525
PspEI GGTNACC 1 cut(s) 1298
PspN4I GGNNCC 3 cut(s) 420, 1260, 1278
PspPI GGNCC 7 cut(s) 16, 971, 1043, 1070, 1097, 1139, 1276
PsuI RGATCY 2 cut(s) 418, 1258
RsaI GTAC 2 cut(s) 1222, 1293
RsaNI GTAC 2 cut(s) 1221, 1292
RseI CAYNNNNRTG 1 cut(s) 1235
SaqAI TTAA 3 cut(s) 344, 408, 657
SatI GCNGC 7 cut(s) 33, 36, 760, 763, 1002, 1203, 1347
Sau3AI GATC 3 cut(s) 418, 648, 1258
Sau96I GGNCC 7 cut(s) 16, 971, 1043, 1070, 1097, 1139, 1276
SchI GAGTC 1 cut(s) 1258
SduI GDGCHC 3 cut(s) 382, 719, 1090
SexAI ACCWGGT 4 cut(s) 628, 1108, 1189, 1216
SfcI CTRYAG 1 cut(s) 465
SinI GGWCC 7 cut(s) 16, 971, 1043, 1070, 1097, 1139, 1276
SmiMI CAYNNNNRTG 1 cut(s) 1235
SmlI CTYRAG 1 cut(s) 1309
SmoI CTYRAG 1 cut(s) 1309
SseBI AGGCCT 2 cut(s) 72, 174
SspI AATATT 1 cut(s) 10
SspMI CTAG 6 cut(s) 44, 375, 921, 993, 1122, 1149
StuI AGGCCT 2 cut(s) 72, 174
StyI CCWWGG 1 cut(s) 43
TaaI ACNGT 2 cut(s) 485, 1304
TauI GCSGC 3 cut(s) 762, 765, 1205
TfiI GAWTC 3 cut(s) 946, 1018, 1327
Tru1I TTAA 3 cut(s) 344, 408, 657
Tru9I TTAA 3 cut(s) 344, 408, 657
TscAI CASTG 1 cut(s) 1242
TseFI GTSAC 2 cut(s) 1228, 1237
TseI GCWGC 4 cut(s) 32, 35, 1001, 1346
Tsp45I GTSAC 2 cut(s) 1228, 1237
TspDTI ATGAA 3 cut(s) 20, 1256, 1340
TspGWI ACGGA 4 cut(s) 740, 779, 788, 797
TspRI CASTG 1 cut(s) 1242
VneI GTGCAC 1 cut(s) 378
VpaK11BI GGWCC 7 cut(s) 16, 971, 1043, 1070, 1097, 1139, 1276
VspI ATTAAT 1 cut(s) 344
XmaJI CCTAGG 1 cut(s) 43
XspI CTAG 6 cut(s) 44, 375, 921, 993, 1122, 1149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.