Prupe.3G224300_v2.0.a1

F-box FBD LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
N/A
Physical Location & Seq
Forward (+)
22377948 .. 22379462
1515 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G224300.1_v2.0.a1.CDS.2

Sequence Viewer

Length: 1224 bp
ATGGAAACCAGAGCTGCAAAGAAGAGAAGGCTTCTTATGGAGCAAGAAAACAAACACAACTTGAAAATCAAGGACGGGCTCAGTGATCTTCCAGATGATGTCTTGCACCACACACTCTCCTTCTTACCCATTAAATCTGCTGCACATACAAGTACTTTATCTAAAAGTTGGTTGCTTGATTGGGTTCCTTGTCTGGTGAAGCATAGGGTTGAAAAACTTGTTCTCTATGTCTGGTTGGTTCACTCGCTTGACTTGCCTTGCTCTCTATTTGAGTGTAACTCACTGAGAAGCCTCACATTGGAAGCCACTCGTTCTGAACATTGCCTCCTAATTAACAATCCCGACACCCGGTTTTGGCTTCCATCTTCTTGTGCTCGGGCTACTAGCGGCCTTCGCTCTCTGTCTTTAACCGGAGTGGATTTCGCAGGCAACGTATTATTTTCAGATCCTTCTTCATTCCCTTCCCTTGAGAAATTGACTGTAGATCATTGTAGAAGAATCAGCCATCTAAAAATATGCTGTGCCAACCTCAAATGCATAGAAGTTTGCAATATGGGTATAAAAAGCCTGGACATATCTGGAATGAAACTAGAGAAGTTGACAGTCAAACTCTGTTTCAACGGATGTGGCAAGGGAAGTAGTGTTAAGATTTTGGCTCCCAATCTACAAACATTTTGTTGGAACGATAACGACTTTGCTGAGAAGTGTTTGATTCAGAGCTTTCCAAAGCTCATGGAAGTTGGGAGCCTTTGTTTGTCCTATCGCATTCTTGAGATTTTGTCGAAAATATATCTTGAATTCGGTGGTCTACCATATTCATTTATGAACCTCAAGACCTTGCAAATTTATACTGATTTGAGAAAAGCTGATATCCCGGCTATAGCTTGCATTTTCAGGAGCTCTCCTGCAGTCCGAAATCTCACAATTACGATAACCGCTGGCTGTGATATCCATAGCCCACCCGCTGGCTGGGAAAATCAAGCTCAAAGTTTGAGCTCCTTCCTATGTCACCTAAAGGTGGTGAATATTGTGGTTTGGACAAATACGATACATGAAAGTGTGATTAACGTAGTGAGGTTTTTGCTTCAACATGGAAGAGCCTTGCAAAAATTTGTCATTTCTTCGTGGCACTCCAAGGTCCATGATCAACTTGCTACGTTATTTCCCCGGGCATCCACTCGTCTCGAAGGCTCATGGTTCTACACTAATTTTGTAAACACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

408

Amino Acids

45.87

Weight (kDa)

8.88

Isoelectric Point (pI)

42.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 965
AccI GTMKAC 1 cut(s) 808
AciI CCGC 3 cut(s) 387, 936, 963
AclWI GGATC 1 cut(s) 440
AcsI RAATTY 3 cut(s) 797, 843, 1109
AfaI GTAC 1 cut(s) 154
AfiI CCNNNNNNNGG 6 cut(s) 298, 348, 354, 965, 969, 1018
AgsI TTSAA 5 cut(s) 64, 212, 619, 797, 1088
AjnI CCWGG 1 cut(s) 567
AloI GAACNNNNNNTCC 2 cut(s) 309, 341
AluBI AGCT 8 cut(s) 14, 720, 730, 866, 884, 900, 983, 996
AluI AGCT 8 cut(s) 14, 720, 730, 866, 884, 900, 983, 996
Alw21I GWGCWC 3 cut(s) 376, 902, 998
Alw26I GTCTC 1 cut(s) 1187
AlwI GGATC 1 cut(s) 440
Ama87I CYCGRG 2 cut(s) 375, 1167
AoxI GGCC 1 cut(s) 388
ApeKI GCWGC 2 cut(s) 14, 140
ApoI RAATTY 3 cut(s) 797, 843, 1109
AspS9I GGNCC 1 cut(s) 1138
AsuC2I CCSGG 4 cut(s) 349, 875, 1168, 1169
AsuHPI GGTGA 3 cut(s) 208, 1001, 1033
AvaI CYCGRG 2 cut(s) 375, 1167
AvaII GGWCC 1 cut(s) 1138
BanII GRGCYC 3 cut(s) 81, 902, 998
Bbv12I GWGCWC 3 cut(s) 376, 902, 998
BbvI GCAGC 1 cut(s) 127
BccI CCATC 2 cut(s) 370, 513
BciT130I CCWGG 1 cut(s) 569
BclI TGATCA 1 cut(s) 1144
BcnI CCSGG 4 cut(s) 349, 875, 1168, 1169
BcoDI GTCTC 1 cut(s) 1187
BfaI CTAG 2 cut(s) 384, 590
BfmI CTRYAG 3 cut(s) 480, 879, 906
BisI GCNGC 3 cut(s) 15, 141, 388
BlsI GCNGC 3 cut(s) 16, 142, 389
BmcAI AGTACT 1 cut(s) 154
Bme1390I CCNGG 5 cut(s) 349, 569, 875, 1168, 1169
Bme18I GGWCC 1 cut(s) 1138
BmeT110I CYCGRG 2 cut(s) 375, 1167
BmgT120I GGNCC 1 cut(s) 1138
BmiI GGNNCC 3 cut(s) 186, 657, 746
BmrFI CCNGG 5 cut(s) 349, 569, 875, 1168, 1169
BmsI GCATC 1 cut(s) 1181
BplI GAGNNNNNCTC 2 cut(s) 263, 295
BpuEI CTTGAG 3 cut(s) 488, 791, 815
BpuMI CCSGG 4 cut(s) 349, 875, 1168, 1169
BsaJI CCNNGG 3 cut(s) 1134, 1166, 1167
BsaWI WCCGGW 1 cut(s) 410
BsaXI ACNNNNNCTCC 6 cut(s) 101, 131, 309, 339, 736, 766
Bsc4I CCNNNNNNNGG 6 cut(s) 298, 348, 354, 965, 969, 1018
Bse3DI GCAATG 1 cut(s) 319
BseBI CCWGG 1 cut(s) 569
BseDI CCNNGG 3 cut(s) 1134, 1166, 1167
BseGI GGATG 2 cut(s) 629, 1172
BseLI CCNNNNNNNGG 6 cut(s) 298, 348, 354, 965, 969, 1018
BseMI GCAATG 1 cut(s) 319
BseMII CTCAG 3 cut(s) 94, 275, 690
BseXI GCAGC 1 cut(s) 127
BseYI CCCAGC 1 cut(s) 969
BsgI GTGCAG 1 cut(s) 126
BshFI GGCC 1 cut(s) 390
BsiHKAI GWGCWC 3 cut(s) 376, 902, 998
BsiHKCI CYCGRG 2 cut(s) 375, 1167
BsiSI CCGG 4 cut(s) 349, 411, 875, 1168
BslI CCNNNNNNNGG 6 cut(s) 298, 348, 354, 965, 969, 1018
BsmAI GTCTC 1 cut(s) 1187
BsmBI CGTCTC 1 cut(s) 1187
BsmI GAATGC 1 cut(s) 765
BsnI GGCC 1 cut(s) 390
BsoBI CYCGRG 2 cut(s) 375, 1167
Bsp1286I GDGCHC 4 cut(s) 81, 376, 902, 998
Bsp143I GATC 4 cut(s) 85, 445, 484, 1144
BspACI CCGC 3 cut(s) 387, 936, 963
BspANI GGCC 1 cut(s) 390
BspCNI CTCAG 3 cut(s) 93, 276, 691
BspLI GGNNCC 3 cut(s) 186, 657, 746
BspMAI CTGCAG 1 cut(s) 910
BspPI GGATC 1 cut(s) 440
BspQI GCTCTTC 1 cut(s) 1090
BsrDI GCAATG 1 cut(s) 319
BssECI CCNNGG 3 cut(s) 1134, 1166, 1167
BssMI GATC 4 cut(s) 85, 445, 484, 1144
BssT1I CCWWGG 1 cut(s) 1134
Bst2UI CCWGG 1 cut(s) 569
Bst4CI ACNGT 2 cut(s) 481, 604
Bst6I CTCTTC 2 cut(s) 17, 1090
BstC8I GCNNGC 4 cut(s) 427, 886, 940, 967
BstDEI CTNAG 3 cut(s) 80, 284, 699
BstF5I GGATG 2 cut(s) 629, 1172
BstKTI GATC 4 cut(s) 88, 448, 487, 1147
BstMAI GTCTC 1 cut(s) 1187
BstMBI GATC 4 cut(s) 85, 445, 484, 1144
BstMWI GCNNNNNNNGC 2 cut(s) 253, 393
BstNI CCWGG 1 cut(s) 569
BstSCI CCNGG 5 cut(s) 347, 567, 873, 1166, 1167
BstSFI CTRYAG 3 cut(s) 480, 879, 906
BstV1I GCAGC 1 cut(s) 127
BstX2I RGATCY 1 cut(s) 445
BstYI RGATCY 1 cut(s) 445
BsuRI GGCC 1 cut(s) 390
BtsCI GGATG 2 cut(s) 629, 1172
BtsIMutI CAGTG 2 cut(s) 88, 281
Cac8I GCNNGC 4 cut(s) 427, 886, 940, 967
Cfr13I GGNCC 1 cut(s) 1138
Cfr9I CCCGGG 1 cut(s) 1167
Csp6I GTAC 1 cut(s) 153
CspCI CAANNNNNGTGG 2 cut(s) 607, 642
CviAII CATG 5 cut(s) 733, 1052, 1091, 1142, 1194
CviQI GTAC 1 cut(s) 153
DdeI CTNAG 3 cut(s) 80, 284, 699
DpnI GATC 4 cut(s) 87, 447, 486, 1146
DpnII GATC 4 cut(s) 85, 445, 484, 1144
Eam1104I CTCTTC 2 cut(s) 17, 1090
EarI CTCTTC 2 cut(s) 17, 1090
Ecl136II GAGCTC 2 cut(s) 900, 996
Eco130I CCWWGG 1 cut(s) 1134
Eco24I GRGCYC 3 cut(s) 81, 902, 998
Eco32I GATATC 2 cut(s) 871, 949
Eco47I GGWCC 1 cut(s) 1138
Eco53kI GAGCTC 2 cut(s) 900, 996
Eco88I CYCGRG 2 cut(s) 375, 1167
EcoICRI GAGCTC 2 cut(s) 900, 996
EcoRI GAATTC 1 cut(s) 797
EcoRII CCWGG 1 cut(s) 567
EcoRV GATATC 2 cut(s) 871, 949
EcoT14I CCWWGG 1 cut(s) 1134
EcoT22I ATGCAT 1 cut(s) 539
EcoT38I GRGCYC 3 cut(s) 81, 902, 998
ErhI CCWWGG 1 cut(s) 1134
Esp3I CGTCTC 1 cut(s) 1187
FaeI CATG 5 cut(s) 736, 1055, 1094, 1145, 1197
FatI CATG 5 cut(s) 732, 1051, 1090, 1141, 1193
FauI CCCGC 1 cut(s) 970
FbaI TGATCA 1 cut(s) 1144
FblI GTMKAC 1 cut(s) 808
Fnu4HI GCNGC 3 cut(s) 15, 141, 388
FokI GGATG 2 cut(s) 636, 1159
FriOI GRGCYC 3 cut(s) 81, 902, 998
Fsp4HI GCNGC 3 cut(s) 15, 141, 388
FspBI CTAG 2 cut(s) 384, 590
GluI GCNGC 3 cut(s) 15, 141, 388
GsaI CCCAGC 1 cut(s) 973
HaeIII GGCC 1 cut(s) 390
HapII CCGG 4 cut(s) 349, 411, 875, 1168
Hin1II CATG 5 cut(s) 736, 1055, 1094, 1145, 1197
HincII GTYRAC 1 cut(s) 600
HindII GTYRAC 1 cut(s) 600
HinfI GANTC 2 cut(s) 498, 712
HpaII CCGG 4 cut(s) 349, 411, 875, 1168
HphI GGTGA 3 cut(s) 208, 1001, 1033
Hpy166II GTNNAC 4 cut(s) 241, 600, 809, 1216
Hpy188I TCNGA 4 cut(s) 316, 445, 717, 914
Hpy188III TCNNGA 8 cut(s) 92, 341, 579, 770, 794, 832, 895, 1184
Hpy8I GTNNAC 4 cut(s) 241, 600, 809, 1216
HpyAV CCTTC 7 cut(s) 21, 130, 401, 459, 471, 1009, 1181
HpyCH4III ACNGT 2 cut(s) 481, 604
HpyCH4IV ACGT 3 cut(s) 432, 1068, 1157
HpyCH4V TGCA 9 cut(s) 17, 106, 143, 537, 549, 841, 888, 908, 1105
HpyF10VI GCNNNNNNNGC 2 cut(s) 253, 393
HpyF3I CTNAG 3 cut(s) 80, 284, 699
HpySE526I ACGT 3 cut(s) 432, 1068, 1157
Hsp92II CATG 5 cut(s) 736, 1055, 1094, 1145, 1197
Ksp22I TGATCA 1 cut(s) 1144
Kzo9I GATC 4 cut(s) 85, 445, 484, 1144
LguI GCTCTTC 1 cut(s) 1090
LmnI GCTCC 5 cut(s) 40, 661, 744, 897, 1001
Lsp1109I GCAGC 1 cut(s) 127
LweI GCATC 1 cut(s) 1181
MaeI CTAG 2 cut(s) 384, 590
MaeII ACGT 3 cut(s) 432, 1068, 1157
MaeIII GTNAC 2 cut(s) 275, 1007
MalI GATC 4 cut(s) 87, 447, 486, 1146
MboI GATC 4 cut(s) 85, 445, 484, 1144
MboII GAAGA 7 cut(s) 34, 80, 357, 444, 507, 1107, 1113
MflI RGATCY 1 cut(s) 445
MhlI GDGCHC 4 cut(s) 81, 376, 902, 998
MluCI AATT 7 cut(s) 330, 473, 797, 843, 924, 1109, 1207
MmeI TCCRAC 1 cut(s) 659
MnlI CCTC 5 cut(s) 302, 335, 539, 839, 1068
Mph1103I ATGCAT 1 cut(s) 539
MseI TTAA 5 cut(s) 132, 333, 407, 645, 1065
MslI CAYNNNNRTG 1 cut(s) 1056
MspA1I CMGCKG 2 cut(s) 938, 965
MspI CCGG 4 cut(s) 349, 411, 875, 1168
MspR9I CCNGG 5 cut(s) 349, 569, 875, 1168, 1169
Mva1269I GAATGC 1 cut(s) 765
MvaI CCWGG 1 cut(s) 569
MwoI GCNNNNNNNGC 2 cut(s) 253, 393
NciI CCSGG 4 cut(s) 349, 875, 1168, 1169
NdeII GATC 4 cut(s) 85, 445, 484, 1144
NlaIII CATG 5 cut(s) 736, 1055, 1094, 1145, 1197
NlaIV GGNNCC 3 cut(s) 186, 657, 746
NmuCI GTSAC 1 cut(s) 1007
NsiI ATGCAT 1 cut(s) 539
PciSI GCTCTTC 1 cut(s) 1090
PcsI WCGNNNNNNNCGW 1 cut(s) 429
PctI GAATGC 1 cut(s) 765
PfeI GAWTC 2 cut(s) 498, 712
PflMI CCANNNNNTGG 1 cut(s) 965
PkrI GCNGC 3 cut(s) 16, 142, 389
Psp124BI GAGCTC 2 cut(s) 902, 998
Psp6I CCWGG 1 cut(s) 567
PspFI CCCAGC 1 cut(s) 969
PspGI CCWGG 1 cut(s) 567
PspN4I GGNNCC 3 cut(s) 186, 657, 746
PspPI GGNCC 1 cut(s) 1138
PstI CTGCAG 1 cut(s) 910
PsuI RGATCY 1 cut(s) 445
RsaI GTAC 1 cut(s) 154
RsaNI GTAC 1 cut(s) 153
RseI CAYNNNNRTG 1 cut(s) 1056
SacI GAGCTC 2 cut(s) 902, 998
SapI GCTCTTC 1 cut(s) 1090
SaqAI TTAA 5 cut(s) 132, 333, 407, 645, 1065
SatI GCNGC 3 cut(s) 15, 141, 388
Sau3AI GATC 4 cut(s) 85, 445, 484, 1144
Sau96I GGNCC 1 cut(s) 1138
ScaI AGTACT 1 cut(s) 154
ScrFI CCNGG 5 cut(s) 349, 569, 875, 1168, 1169
SduI GDGCHC 4 cut(s) 81, 376, 902, 998
SfaNI GCATC 1 cut(s) 1181
SfcI CTRYAG 3 cut(s) 480, 879, 906
SinI GGWCC 1 cut(s) 1138
SmaI CCCGGG 1 cut(s) 1169
SmiMI CAYNNNNRTG 1 cut(s) 1056
SmlI CTYRAG 3 cut(s) 467, 770, 830
SmoI CTYRAG 3 cut(s) 467, 770, 830
Sse9I AATT 7 cut(s) 330, 473, 797, 843, 924, 1109, 1207
SsiI CCGC 3 cut(s) 387, 936, 963
SspI AATATT 1 cut(s) 1027
SspMI CTAG 2 cut(s) 384, 590
SstI GAGCTC 2 cut(s) 902, 998
StyD4I CCNGG 5 cut(s) 347, 567, 873, 1166, 1167
StyI CCWWGG 1 cut(s) 1134
TaaI ACNGT 2 cut(s) 481, 604
TaiI ACGT 3 cut(s) 435, 1071, 1160
TaqI TCGA 2 cut(s) 782, 1185
TasI AATT 7 cut(s) 330, 473, 797, 843, 924, 1109, 1207
TatI WGTACW 1 cut(s) 152
TauI GCSGC 1 cut(s) 390
TfiI GAWTC 2 cut(s) 498, 712
Tru1I TTAA 5 cut(s) 132, 333, 407, 645, 1065
Tru9I TTAA 5 cut(s) 132, 333, 407, 645, 1065
TscAI CASTG 2 cut(s) 88, 288
TseFI GTSAC 1 cut(s) 1007
TseI GCWGC 2 cut(s) 14, 140
Tsp45I GTSAC 1 cut(s) 1007
TspDTI ATGAA 5 cut(s) 444, 599, 807, 839, 1068
TspGWI ACGGA 1 cut(s) 636
TspMI CCCGGG 1 cut(s) 1167
TspRI CASTG 2 cut(s) 88, 288
Van91I CCANNNNNTGG 1 cut(s) 965
VpaK11BI GGWCC 1 cut(s) 1138
XapI RAATTY 3 cut(s) 797, 843, 1109
XcmI CCANNNNNNNNNTGG 1 cut(s) 966
XmaI CCCGGG 1 cut(s) 1167
XmiI GTMKAC 1 cut(s) 808
XspI CTAG 2 cut(s) 384, 590
ZrmI AGTACT 1 cut(s) 154
Zsp2I ATGCAT 1 cut(s) 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.