Prupe.3G251300_v2.0.a1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
N/A
Physical Location & Seq
Forward (+)
24121345 .. 24121995
651 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G251300.1_v2.0.a1.CDS.1

Sequence Viewer

Length: 486 bp
ATGTGGATCCATATCGAATTCATTAGAAATATGGGATCATCAGTCGTCGTAGGTGTTTTTGTTATTATGCTGACCACAGCGATGGTGTCTCAGCTGCCCAGCACAAGCCATTGCCTGATGAACAAGAGCAGCAGCAGCACTGGCTCCTTCTCTAACTGGTGCATTAATGGGAGCACCCACCAATATTGCCAGCAGCTTATTATTCCACAGGAGGAGGAGGACGTAGATCAGTTGGAGTCGGAGGCTATGAATATGATCATGAAGCATCTGCTGGTGGTGGATGATTCACATCACGAAATTAGCGACGAAATTAGCAACAGAATGCTGCTTGTCCGTCATGATAGCAAAATTACTCAAGAATCGTTTATAAAGTACAAGCCGGTAGTTAACTGTGGCAGAAGACTACTAAAACTATATCCTCCTTTCCCAAAAAGGAATAAGGGCACGAGGCTTTCTTCCGAAATTTCGGCAGAGTCTCCCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

162

Amino Acids

18.31

Weight (kDa)

6.37

Isoelectric Point (pI)

58.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 368
AclWI GGATC 2 cut(s) 14, 43
AcsI RAATTY 2 cut(s) 17, 462
AfaI GTAC 1 cut(s) 374
AluBI AGCT 2 cut(s) 94, 196
AluI AGCT 2 cut(s) 94, 196
Alw21I GWGCWC 1 cut(s) 176
Alw26I GTCTC 2 cut(s) 93, 480
AlwI GGATC 2 cut(s) 14, 43
ApeKI GCWGC 6 cut(s) 94, 129, 132, 135, 193, 325
ApoI RAATTY 2 cut(s) 17, 462
AseI ATTAAT 1 cut(s) 165
BaeGI GKGCMC 1 cut(s) 446
BamHI GGATCC 1 cut(s) 6
BauI CACGAG 1 cut(s) 445
BbsI GAAGAC 1 cut(s) 406
Bbv12I GWGCWC 1 cut(s) 176
BbvI GCAGC 6 cut(s) 81, 141, 144, 147, 205, 312
BccI CCATC 1 cut(s) 76
BclI TGATCA 1 cut(s) 255
BcoDI GTCTC 2 cut(s) 93, 480
BisI GCNGC 6 cut(s) 95, 130, 133, 136, 194, 326
BlsI GCNGC 6 cut(s) 96, 131, 134, 137, 195, 327
BmiI GGNNCC 2 cut(s) 8, 145
BmsI GCATC 1 cut(s) 274
BpiI GAAGAC 1 cut(s) 406
BpuEI CTTGAG 1 cut(s) 339
BsaBI GATNNNNATC 2 cut(s) 11, 288
Bse118I RCCGGY 1 cut(s) 379
Bse1I ACTGG 2 cut(s) 145, 161
Bse3DI GCAATG 1 cut(s) 109
Bse8I GATNNNNATC 2 cut(s) 11, 288
BseGI GGATG 1 cut(s) 286
BseJI GATNNNNATC 2 cut(s) 11, 288
BseMI GCAATG 1 cut(s) 109
BseMII CTCAG 1 cut(s) 104
BseNI ACTGG 2 cut(s) 145, 161
BseRI GAGGAG 2 cut(s) 227, 230
BseSI GKGCMC 1 cut(s) 446
BseXI GCAGC 6 cut(s) 81, 141, 144, 147, 205, 312
BseYI CCCAGC 1 cut(s) 98
BsiHKAI GWGCWC 1 cut(s) 176
BsiSI CCGG 1 cut(s) 380
BsmAI GTCTC 2 cut(s) 93, 480
BsmI GAATGC 1 cut(s) 327
Bsp1286I GDGCHC 2 cut(s) 176, 446
Bsp143I GATC 4 cut(s) 6, 35, 226, 255
BspCNI CTCAG 1 cut(s) 103
BspHI TCATGA 2 cut(s) 258, 337
BspLI GGNNCC 2 cut(s) 8, 145
BspPI GGATC 2 cut(s) 14, 43
BsrDI GCAATG 1 cut(s) 109
BsrFI RCCGGY 1 cut(s) 379
BsrI ACTGG 2 cut(s) 145, 161
BssAI RCCGGY 1 cut(s) 379
BssMI GATC 4 cut(s) 6, 35, 226, 255
BssSI CACGAG 1 cut(s) 445
Bst2BI CACGAG 1 cut(s) 445
Bst4CI ACNGT 1 cut(s) 392
BstC8I GCNNGC 1 cut(s) 191
BstDEI CTNAG 1 cut(s) 90
BstF5I GGATG 1 cut(s) 286
BstKTI GATC 4 cut(s) 9, 38, 229, 258
BstMAI GTCTC 2 cut(s) 93, 480
BstMBI GATC 4 cut(s) 6, 35, 226, 255
BstMWI GCNNNNNNNGC 2 cut(s) 135, 141
BstSLI GKGCMC 1 cut(s) 446
BstV1I GCAGC 6 cut(s) 81, 141, 144, 147, 205, 312
BstV2I GAAGAC 1 cut(s) 406
BstX2I RGATCY 1 cut(s) 6
BstXI CCANNNNNNTGG 1 cut(s) 82
BstYI RGATCY 1 cut(s) 6
BtgZI GCGATG 1 cut(s) 95
BtsCI GGATG 1 cut(s) 286
BtsIMutI CAGTG 1 cut(s) 138
Cac8I GCNNGC 1 cut(s) 191
CciI TCATGA 2 cut(s) 258, 337
Cfr10I RCCGGY 1 cut(s) 379
Csp6I GTAC 1 cut(s) 373
CspCI CAANNNNNGTGG 2 cut(s) 167, 202
CviAII CATG 2 cut(s) 259, 338
CviJI RGCY 7 cut(s) 94, 108, 144, 196, 245, 379, 451
CviKI_1 RGCY 7 cut(s) 94, 108, 144, 196, 245, 379, 451
CviQI GTAC 1 cut(s) 373
DdeI CTNAG 1 cut(s) 90
DpnI GATC 4 cut(s) 8, 37, 228, 257
DpnII GATC 4 cut(s) 6, 35, 226, 255
EcoRI GAATTC 1 cut(s) 17
FaeI CATG 2 cut(s) 262, 341
FaiI YATR 9 cut(s) 12, 32, 68, 248, 254, 260, 339, 368, 415
FatI CATG 2 cut(s) 258, 337
FbaI TGATCA 1 cut(s) 255
Fnu4HI GCNGC 6 cut(s) 95, 130, 133, 136, 194, 326
FokI GGATG 1 cut(s) 293
Fsp4HI GCNGC 6 cut(s) 95, 130, 133, 136, 194, 326
GluI GCNGC 6 cut(s) 95, 130, 133, 136, 194, 326
GsaI CCCAGC 1 cut(s) 102
HapII CCGG 1 cut(s) 380
Hin1II CATG 2 cut(s) 262, 341
HincII GTYRAC 1 cut(s) 388
HindII GTYRAC 1 cut(s) 388
HinfI GANTC 4 cut(s) 236, 284, 359, 473
HpaI GTTAAC 1 cut(s) 388
HpaII CCGG 1 cut(s) 380
Hpy166II GTNNAC 1 cut(s) 388
Hpy188I TCNGA 2 cut(s) 241, 460
Hpy188III TCNNGA 4 cut(s) 259, 293, 338, 356
Hpy8I GTNNAC 1 cut(s) 388
Hpy99I CGWCG 2 cut(s) 50, 308
HpyAV CCTTC 1 cut(s) 157
HpyCH4III ACNGT 1 cut(s) 392
HpyCH4IV ACGT 1 cut(s) 222
HpyCH4V TGCA 1 cut(s) 162
HpyF10VI GCNNNNNNNGC 2 cut(s) 135, 141
HpyF3I CTNAG 1 cut(s) 90
HpySE526I ACGT 1 cut(s) 222
Hsp92II CATG 2 cut(s) 262, 341
Ksp22I TGATCA 1 cut(s) 255
KspAI GTTAAC 1 cut(s) 388
Kzo9I GATC 4 cut(s) 6, 35, 226, 255
LmnI GCTCC 2 cut(s) 149, 171
LpnPI CCDG 8 cut(s) 112, 126, 128, 142, 194, 203, 257, 393
Lsp1109I GCAGC 6 cut(s) 81, 141, 144, 147, 205, 312
LweI GCATC 1 cut(s) 274
MaeII ACGT 1 cut(s) 222
MalI GATC 4 cut(s) 8, 37, 228, 257
MboI GATC 4 cut(s) 6, 35, 226, 255
MboII GAAGA 2 cut(s) 411, 447
MflI RGATCY 1 cut(s) 6
MhlI GDGCHC 2 cut(s) 176, 446
MluCI AATT 5 cut(s) 17, 297, 309, 348, 462
MlyI GAGTC 2 cut(s) 245, 482
MmeI TCCRAC 2 cut(s) 213, 219
MnlI CCTC 6 cut(s) 205, 208, 211, 235, 429, 441
MseI TTAA 2 cut(s) 165, 387
MslI CAYNNNNRTG 1 cut(s) 80
MspA1I CMGCKG 1 cut(s) 94
MspI CCGG 1 cut(s) 380
Mva1269I GAATGC 1 cut(s) 327
MwoI GCNNNNNNNGC 2 cut(s) 135, 141
NdeII GATC 4 cut(s) 6, 35, 226, 255
NlaIII CATG 2 cut(s) 262, 341
NlaIV GGNNCC 2 cut(s) 8, 145
PagI TCATGA 2 cut(s) 258, 337
PcsI WCGNNNNNNNCGW 1 cut(s) 300
PctI GAATGC 1 cut(s) 327
PfeI GAWTC 2 cut(s) 284, 359
PkrI GCNGC 6 cut(s) 96, 131, 134, 137, 195, 327
PleI GAGTC 2 cut(s) 244, 481
PpsI GAGTC 2 cut(s) 244, 481
PshBI ATTAAT 1 cut(s) 165
PsiI TTATAA 1 cut(s) 368
PspFI CCCAGC 1 cut(s) 98
PspN4I GGNNCC 2 cut(s) 8, 145
PsuI RGATCY 1 cut(s) 6
PvuII CAGCTG 1 cut(s) 94
RsaI GTAC 1 cut(s) 374
RsaNI GTAC 1 cut(s) 373
RseI CAYNNNNRTG 1 cut(s) 80
SaqAI TTAA 2 cut(s) 165, 387
SatI GCNGC 6 cut(s) 95, 130, 133, 136, 194, 326
Sau3AI GATC 4 cut(s) 6, 35, 226, 255
SchI GAGTC 2 cut(s) 245, 482
SduI GDGCHC 2 cut(s) 176, 446
SetI ASST 4 cut(s) 55, 96, 198, 225
SfaNI GCATC 1 cut(s) 274
SmiMI CAYNNNNRTG 1 cut(s) 80
SmlI CTYRAG 1 cut(s) 354
SmoI CTYRAG 1 cut(s) 354
Sse9I AATT 5 cut(s) 17, 297, 309, 348, 462
SspI AATATT 1 cut(s) 185
TaaI ACNGT 1 cut(s) 392
TaiI ACGT 1 cut(s) 225
TaqI TCGA 1 cut(s) 15
TasI AATT 5 cut(s) 17, 297, 309, 348, 462
TatI WGTACW 1 cut(s) 372
TfiI GAWTC 2 cut(s) 284, 359
Tru1I TTAA 2 cut(s) 165, 387
Tru9I TTAA 2 cut(s) 165, 387
TscAI CASTG 1 cut(s) 145
TseI GCWGC 6 cut(s) 94, 129, 132, 135, 193, 325
TspDTI ATGAA 4 cut(s) 10, 134, 263, 275
TspGWI ACGGA 1 cut(s) 323
TspRI CASTG 1 cut(s) 145
VspI ATTAAT 1 cut(s) 165
XapI RAATTY 2 cut(s) 17, 462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.