Prupe.3G277300_v2.0.a1

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
N/A
Physical Location & Seq
Reverse (-)
25489607 .. 25490443
837 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G277300.1_v2.0.a1.CDS.2

Sequence Viewer

Length: 750 bp
ATGACCTCGTCTCCGTTCGAGTTCCTTGAGCATCTCAAGGGGTGCCACAAGGGTGACAAGGTCCAAGGCATCCAAGACCTCAAAAAATACCTTGGAAAATTTGGTTACTTGAGCAGCAACAATGGTCACCTCAATGATGATGATTTTGACGACCAATTGGAGTCTGCCATCAAAACTTACCAAATTAATTACCACCTCAAAGCCACCGGAACATTGGATGCCAAAACCGTATCCAACATGATGATGCCACGTTGTGGTGTTGCAGATATCATCAATGAAAACCCAACATGGCCGGCCTCTAAGTATCACCTCACCTATGCTTTTCTTCAAGGCACCCCAGCTGAGGCCATGGGCCCAGTTTCCAGTGCTTTTCAAACATGGGCCGCCAACACACACTTCACCTTCAGTGAGGCACAGAACAACCAGAACCCAGATCTGACGGTCAGTTTTAACAGGGGCGATCATGGAGATGGGACCCCATTTGATGGGCCAGGTGGGACCATAGCCCATGCATTTGCGCCGACAAATGGGAGACTTCATTACGACGCTGATGAGCGGTTTTCCGTGGGCGCCGTAAGTGGTGCTTATGACTTTGAGACTGTGGCTTTGCATGAAATAGGGCACCTTCTTGGGCTCAGGCATAGCTCTGTTCAAGGAGCTATTATGTTCCCCTCTGTCCGCGCTGGAGTGACCCAGCAGAGCTTGCATGGGGATGATATTCAAGGAATTAAAGCTTTATACAACACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

250

Amino Acids

27.14

Weight (kDa)

5.63

Isoelectric Point (pI)

23.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 4 cut(s) 42, 332, 569, 621
AccB7I CCANNNNNTGG 2 cut(s) 254, 485
AccBSI CCGCTC 1 cut(s) 556
AccII CGCG 1 cut(s) 681
AciI CCGC 3 cut(s) 384, 556, 679
AcoI YGGCCR 1 cut(s) 290
AcsI RAATTY 1 cut(s) 98
AcuI CTGAAG 1 cut(s) 388
AcyI GRCGYC 1 cut(s) 570
AdeI CACNNNGTG 1 cut(s) 254
AfiI CCNNNNNNNGG 4 cut(s) 254, 343, 485, 527
AgsI TTSAA 4 cut(s) 329, 374, 653, 722
AjnI CCWGG 1 cut(s) 490
AjuI GAANNNNNNNTTGG 2 cut(s) 380, 412
AleI CACNNNNGTG 1 cut(s) 51
AluBI AGCT 5 cut(s) 341, 645, 659, 702, 734
AluI AGCT 5 cut(s) 341, 645, 659, 702, 734
Alw26I GTCTC 3 cut(s) 15, 526, 590
AoxI GGCC 6 cut(s) 290, 294, 345, 352, 381, 488
ApaI GGGCCC 1 cut(s) 356
ApeKI GCWGC 1 cut(s) 114
ApoI RAATTY 1 cut(s) 98
ArsI GACNNNNNNTTYG 2 cut(s) 589, 621
AseI ATTAAT 1 cut(s) 186
AspLEI GCGC 3 cut(s) 520, 572, 683
AspS9I GGNCC 7 cut(s) 61, 352, 353, 381, 474, 488, 498
AsuHPI GGTGA 5 cut(s) 65, 119, 299, 304, 391
AvaII GGWCC 3 cut(s) 61, 474, 498
BaeGI GKGCMC 2 cut(s) 356, 624
BanI GGYRCC 4 cut(s) 42, 332, 569, 621
BanII GRGCYC 2 cut(s) 356, 636
BbvCI CCTCAGC 1 cut(s) 342
BbvI GCAGC 1 cut(s) 126
BccI CCATC 3 cut(s) 176, 464, 479
BceAI ACGGC 1 cut(s) 557
BciT130I CCWGG 1 cut(s) 492
BciVI GTATCC 1 cut(s) 241
BcoDI GTCTC 3 cut(s) 15, 526, 590
BfoI RGCGCY 1 cut(s) 573
BfuI GTATCC 1 cut(s) 241
BglII AGATCT 1 cut(s) 433
BisI GCNGC 2 cut(s) 115, 384
BlsI GCNGC 2 cut(s) 116, 385
Bme1390I CCNGG 1 cut(s) 492
Bme18I GGWCC 3 cut(s) 61, 474, 498
BmgT120I GGNCC 7 cut(s) 61, 352, 353, 381, 474, 488, 498
BmiI GGNNCC 8 cut(s) 44, 334, 354, 475, 476, 499, 571, 623
BmrFI CCNGG 1 cut(s) 492
BmrI ACTGGG 1 cut(s) 350
BmsI GCATC 4 cut(s) 40, 78, 208, 234
BmuI ACTGGG 1 cut(s) 350
BpmI CTGGAG 1 cut(s) 705
Bpu10I CCTNAGC 2 cut(s) 342, 635
BpuEI CTTGAG 3 cut(s) 20, 47, 130
BsaHI GRCGYC 1 cut(s) 570
BsaJI CCNNGG 4 cut(s) 64, 91, 348, 564
BsaWI WCCGGW 1 cut(s) 206
BsaXI ACNNNNNCTCC 1 cut(s) 25
Bsc4I CCNNNNNNNGG 4 cut(s) 254, 343, 485, 527
Bse118I RCCGGY 1 cut(s) 292
Bse1I ACTGG 2 cut(s) 356, 363
BseBI CCWGG 1 cut(s) 492
BseDI CCNNGG 4 cut(s) 64, 91, 348, 564
BseGI GGATG 3 cut(s) 69, 223, 718
BseLI CCNNNNNNNGG 4 cut(s) 254, 343, 485, 527
BseMII CTCAG 2 cut(s) 333, 649
BseNI ACTGG 2 cut(s) 356, 363
BseSI GKGCMC 2 cut(s) 356, 624
BseXI GCAGC 1 cut(s) 126
BseYI CCCAGC 2 cut(s) 337, 693
Bsh1236I CGCG 1 cut(s) 681
BshFI GGCC 6 cut(s) 292, 296, 347, 354, 383, 490
BshNI GGYRCC 4 cut(s) 42, 332, 569, 621
BsiSI CCGG 2 cut(s) 207, 293
BslFI GGGAC 2 cut(s) 487, 511
BslI CCNNNNNNNGG 4 cut(s) 254, 343, 485, 527
BsmAI GTCTC 3 cut(s) 15, 526, 590
BsmBI CGTCTC 1 cut(s) 15
BsmFI GGGAC 2 cut(s) 487, 511
BsnI GGCC 6 cut(s) 292, 296, 347, 354, 383, 490
Bsp120I GGGCCC 1 cut(s) 352
Bsp1286I GDGCHC 3 cut(s) 356, 624, 636
Bsp143I GATC 2 cut(s) 433, 460
Bsp19I CCATGG 1 cut(s) 348
BspACI CCGC 3 cut(s) 384, 556, 679
BspANI GGCC 6 cut(s) 292, 296, 347, 354, 383, 490
BspCNI CTCAG 2 cut(s) 334, 648
BspFNI CGCG 1 cut(s) 681
BspLI GGNNCC 8 cut(s) 44, 334, 354, 475, 476, 499, 571, 623
BspT107I GGYRCC 4 cut(s) 42, 332, 569, 621
BsrBI CCGCTC 1 cut(s) 556
BsrFI RCCGGY 1 cut(s) 292
BsrI ACTGG 2 cut(s) 356, 363
BssAI RCCGGY 1 cut(s) 292
BssECI CCNNGG 4 cut(s) 64, 91, 348, 564
BssMI GATC 2 cut(s) 433, 460
BssNI GRCGYC 1 cut(s) 570
BssT1I CCWWGG 3 cut(s) 64, 91, 348
Bst2UI CCWGG 1 cut(s) 492
Bst4CI ACNGT 3 cut(s) 229, 442, 601
BstACI GRCGYC 1 cut(s) 570
BstAPI GCANNNNNTGC 1 cut(s) 703
BstC8I GCNNGC 2 cut(s) 294, 704
BstDEI CTNAG 3 cut(s) 300, 342, 635
BstDSI CCRYGG 2 cut(s) 348, 564
BstEII GGTNACC 1 cut(s) 125
BstF5I GGATG 3 cut(s) 69, 223, 718
BstFNI CGCG 1 cut(s) 681
BstH2I RGCGCY 1 cut(s) 573
BstHHI GCGC 3 cut(s) 520, 572, 683
BstKTI GATC 2 cut(s) 436, 463
BstMAI GTCTC 3 cut(s) 15, 526, 590
BstMBI GATC 2 cut(s) 433, 460
BstMWI GCNNNNNNNGC 1 cut(s) 703
BstNI CCWGG 1 cut(s) 492
BstPI GGTNACC 1 cut(s) 125
BstSCI CCNGG 1 cut(s) 490
BstSLI GKGCMC 2 cut(s) 356, 624
BstUI CGCG 1 cut(s) 681
BstV1I GCAGC 1 cut(s) 126
BstX2I RGATCY 1 cut(s) 433
BstYI RGATCY 1 cut(s) 433
BsuI GTATCC 1 cut(s) 241
BsuRI GGCC 6 cut(s) 292, 296, 347, 354, 383, 490
BtgI CCRYGG 2 cut(s) 348, 564
BtsCI GGATG 3 cut(s) 69, 223, 718
BtsIMutI CAGTG 2 cut(s) 370, 412
Cac8I GCNNGC 2 cut(s) 294, 704
CfoI GCGC 3 cut(s) 520, 572, 683
Cfr10I RCCGGY 1 cut(s) 292
Cfr13I GGNCC 7 cut(s) 61, 352, 353, 381, 474, 488, 498
CseI GACGC 1 cut(s) 554
CviAII CATG 8 cut(s) 238, 288, 349, 378, 464, 509, 611, 707
DdeI CTNAG 3 cut(s) 300, 342, 635
DinI GGCGCC 1 cut(s) 571
DpnI GATC 2 cut(s) 435, 462
DpnII GATC 2 cut(s) 433, 460
DraIII CACNNNGTG 1 cut(s) 254
EaeI YGGCCR 1 cut(s) 290
Eco130I CCWWGG 3 cut(s) 64, 91, 348
Eco24I GRGCYC 2 cut(s) 356, 636
Eco32I GATATC 1 cut(s) 268
Eco47I GGWCC 3 cut(s) 61, 474, 498
Eco57I CTGAAG 1 cut(s) 388
Eco91I GGTNACC 1 cut(s) 125
EcoO109I RGGNCCY 1 cut(s) 474
EcoO65I GGTNACC 1 cut(s) 125
EcoRII CCWGG 1 cut(s) 490
EcoRV GATATC 1 cut(s) 268
EcoT14I CCWWGG 3 cut(s) 64, 91, 348
EcoT22I ATGCAT 1 cut(s) 514
EcoT38I GRGCYC 2 cut(s) 356, 636
EgeI GGCGCC 1 cut(s) 571
EheI GGCGCC 1 cut(s) 571
ErhI CCWWGG 3 cut(s) 64, 91, 348
Esp3I CGTCTC 1 cut(s) 15
FaeI CATG 8 cut(s) 241, 291, 352, 381, 467, 512, 614, 710
FalI AAGNNNNNCTT 2 cut(s) 568, 600
FaqI GGGAC 2 cut(s) 487, 511
FatI CATG 8 cut(s) 237, 287, 348, 377, 463, 508, 610, 706
Fnu4HI GCNGC 2 cut(s) 115, 384
FokI GGATG 3 cut(s) 56, 230, 725
FriOI GRGCYC 2 cut(s) 356, 636
FseI GGCCGGCC 1 cut(s) 296
Fsp4HI GCNGC 2 cut(s) 115, 384
GlaI GCGC 3 cut(s) 519, 571, 682
GluI GCNGC 2 cut(s) 115, 384
GsaI CCCAGC 2 cut(s) 341, 697
GsuI CTGGAG 1 cut(s) 705
HaeII RGCGCY 1 cut(s) 573
HaeIII GGCC 6 cut(s) 292, 296, 347, 354, 383, 490
HapII CCGG 2 cut(s) 207, 293
HgaI GACGC 1 cut(s) 554
HhaI GCGC 3 cut(s) 520, 572, 683
Hin1I GRCGYC 1 cut(s) 570
Hin1II CATG 8 cut(s) 241, 291, 352, 381, 467, 512, 614, 710
Hin6I GCGC 3 cut(s) 518, 570, 681
HinP1I GCGC 3 cut(s) 518, 570, 681
HindIII AAGCTT 1 cut(s) 732
HinfI GANTC 1 cut(s) 161
HpaII CCGG 2 cut(s) 207, 293
HphI GGTGA 5 cut(s) 65, 119, 299, 304, 391
Hpy188I TCNGA 1 cut(s) 438
Hpy99I CGWCG 1 cut(s) 548
HpyAV CCTTC 2 cut(s) 412, 635
HpyCH4III ACNGT 3 cut(s) 229, 442, 601
HpyCH4IV ACGT 1 cut(s) 250
HpyCH4V TGCA 4 cut(s) 263, 512, 610, 706
HpyF10VI GCNNNNNNNGC 1 cut(s) 703
HpyF3I CTNAG 3 cut(s) 300, 342, 635
HpySE526I ACGT 1 cut(s) 250
Hsp92I GRCGYC 1 cut(s) 570
Hsp92II CATG 8 cut(s) 241, 291, 352, 381, 467, 512, 614, 710
HspAI GCGC 3 cut(s) 518, 570, 681
KasI GGCGCC 1 cut(s) 569
KflI GGGWCCC 1 cut(s) 474
KroI GCCGGC 1 cut(s) 292
KroNI GCCGGC 1 cut(s) 294
Kzo9I GATC 2 cut(s) 433, 460
LmnI GCTCC 1 cut(s) 656
Lsp1109I GCAGC 1 cut(s) 126
LweI GCATC 4 cut(s) 40, 78, 208, 234
MaeII ACGT 1 cut(s) 250
MaeIII GTNAC 4 cut(s) 53, 104, 125, 688
MalI GATC 2 cut(s) 435, 462
MbiI CCGCTC 1 cut(s) 556
MboI GATC 2 cut(s) 433, 460
MboII GAAGA 1 cut(s) 317
MfeI CAATTG 1 cut(s) 155
MflI RGATCY 1 cut(s) 433
MhlI GDGCHC 3 cut(s) 356, 624, 636
MluCI AATT 5 cut(s) 98, 155, 183, 187, 726
Mly113I GGCGCC 1 cut(s) 570
MlyI GAGTC 1 cut(s) 170
MmeI TCCRAC 1 cut(s) 258
MnlI CCTC 9 cut(s) 16, 89, 140, 206, 307, 320, 337, 403, 682
Mph1103I ATGCAT 1 cut(s) 514
MroNI GCCGGC 1 cut(s) 292
MseI TTAA 3 cut(s) 186, 450, 729
MslI CAYNNNNRTG 5 cut(s) 51, 132, 242, 468, 711
MspA1I CMGCKG 1 cut(s) 341
MspI CCGG 2 cut(s) 207, 293
MspR9I CCNGG 1 cut(s) 492
MunI CAATTG 1 cut(s) 155
MvaI CCWGG 1 cut(s) 492
MvnI CGCG 1 cut(s) 681
MwoI GCNNNNNNNGC 1 cut(s) 703
NaeI GCCGGC 1 cut(s) 294
NarI GGCGCC 1 cut(s) 570
NcoI CCATGG 1 cut(s) 348
NdeII GATC 2 cut(s) 433, 460
NgoMIV GCCGGC 1 cut(s) 292
NlaIII CATG 8 cut(s) 241, 291, 352, 381, 467, 512, 614, 710
NlaIV GGNNCC 8 cut(s) 44, 334, 354, 475, 476, 499, 571, 623
NmuCI GTSAC 3 cut(s) 53, 125, 688
NsiI ATGCAT 1 cut(s) 514
OliI CACNNNNGTG 1 cut(s) 51
PdiI GCCGGC 1 cut(s) 294
PflFI GACNNNGTC 2 cut(s) 7, 59
PflMI CCANNNNNTGG 2 cut(s) 254, 485
PkrI GCNGC 2 cut(s) 116, 385
PleI GAGTC 1 cut(s) 169
PluTI GGCGCC 1 cut(s) 573
PpsI GAGTC 1 cut(s) 169
PpuMI RGGWCCY 1 cut(s) 474
PshBI ATTAAT 1 cut(s) 186
Psp5II RGGWCCY 1 cut(s) 474
Psp6I CCWGG 1 cut(s) 490
PspEI GGTNACC 1 cut(s) 125
PspFI CCCAGC 2 cut(s) 337, 693
PspGI CCWGG 1 cut(s) 490
PspN4I GGNNCC 8 cut(s) 44, 334, 354, 475, 476, 499, 571, 623
PspOMI GGGCCC 1 cut(s) 352
PspPI GGNCC 7 cut(s) 61, 352, 353, 381, 474, 488, 498
PspPPI RGGWCCY 1 cut(s) 474
PsuI RGATCY 1 cut(s) 433
PsyI GACNNNGTC 2 cut(s) 7, 59
PvuII CAGCTG 1 cut(s) 341
RigI GGCCGGCC 1 cut(s) 296
RseI CAYNNNNRTG 5 cut(s) 51, 132, 242, 468, 711
SaqAI TTAA 3 cut(s) 186, 450, 729
SatI GCNGC 2 cut(s) 115, 384
Sau3AI GATC 2 cut(s) 433, 460
Sau96I GGNCC 7 cut(s) 61, 352, 353, 381, 474, 488, 498
SchI GAGTC 1 cut(s) 170
ScrFI CCNGG 1 cut(s) 492
SduI GDGCHC 3 cut(s) 356, 624, 636
SfaNI GCATC 4 cut(s) 40, 78, 208, 234
SfoI GGCGCC 1 cut(s) 571
SinI GGWCC 3 cut(s) 61, 474, 498
SmiMI CAYNNNNRTG 5 cut(s) 51, 132, 242, 468, 711
SmlI CTYRAG 3 cut(s) 26, 35, 109
SmoI CTYRAG 3 cut(s) 26, 35, 109
Sse9I AATT 5 cut(s) 98, 155, 183, 187, 726
SsiI CCGC 3 cut(s) 384, 556, 679
SspDI GGCGCC 1 cut(s) 569
StyD4I CCNGG 1 cut(s) 490
StyI CCWWGG 3 cut(s) 64, 91, 348
TaaI ACNGT 3 cut(s) 229, 442, 601
TaiI ACGT 1 cut(s) 253
TaqI TCGA 1 cut(s) 18
TasI AATT 5 cut(s) 98, 155, 183, 187, 726
TauI GCSGC 1 cut(s) 386
Tru1I TTAA 3 cut(s) 186, 450, 729
Tru9I TTAA 3 cut(s) 186, 450, 729
TscAI CASTG 2 cut(s) 370, 412
TseFI GTSAC 3 cut(s) 53, 125, 688
TseI GCWGC 1 cut(s) 114
Tsp45I GTSAC 3 cut(s) 53, 125, 688
TspDTI ATGAA 3 cut(s) 291, 527, 627
TspGWI ACGGA 1 cut(s) 553
TspRI CASTG 2 cut(s) 370, 412
Tth111I GACNNNGTC 2 cut(s) 7, 59
Van91I CCANNNNNTGG 2 cut(s) 254, 485
VpaK11BI GGWCC 3 cut(s) 61, 474, 498
VspI ATTAAT 1 cut(s) 186
XapI RAATTY 1 cut(s) 98
XcmI CCANNNNNNNNNTGG 1 cut(s) 211
Zsp2I ATGCAT 1 cut(s) 514
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.