Prupe.3G277500_v2.0.a1

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
N/A
Physical Location & Seq
Reverse (-)
25494112 .. 25495044
933 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G277500.1_v2.0.a1.CDS.1

Sequence Viewer

Length: 933 bp
ATGGCATCAAAGTCTCATCTTTGTCTCTTGGCATGCACTCTCCTCTTCATCCTCCTTTCTCTCCCCTCCCATGCAAATTCATCCGAAACCCACAACCAAAAAACATCACCATTTGAGTTCCTTGAACATCTTAAGGGGTTCCAAAATGGTGACAAGGTCCCAGGAATCCAAGACCTCAAAAAATACCTCGGAAGGTTTGGTTACTTAAGCAGCAACAATAACCAAACCACTGATGATTTTGACGATCAATTGGCGTCGGCCATCAAAACCTACCAACTCAATTACAACCTCAAAACCACTGGAATATTGGACGCTCAAACAGTATCCAATATGATGATGCCACGTTGTGGCGTCCCAGATATCATCAATGGTACCTCCTCTATGCGATCAGGCAAGCAAAGTCACCCTCCTCCGCACCACCATCACAAGGGACACACAGTTGCTCATTATACTTTCTTCCCTGGAAAGCCAAAATGGCCAGCCTCTAAGTACCACCTCACCTACGCTTTTCTTCGGGGCACCCCGACGGCGGCCAAGGGCCCAGTTGCACGTGCTTTTAATACGTGGGCTGCCCACACACAGTTCAAGTTCAGCCAGGCTCGAAGGAATCAGAAGCCGGATCTGACCGTAAGTTTTCACCGGCGTAGCCATGGAGATGGGCATCCATTTGATGGCCCAGGAGGGATCCTAGCGCATTCTTTCGCGCCGAAAGATGGGAGATTTCACTATGACGCTGATGAGAAGTGGTCTGTGGGCGTTGTGAGGGGTGCTTTTCACTTGGAGACTGTGGCTTTGCATGAAATTGGTCACCTCCTAGGGCTTGGCCATAGCTCTGTTCGGAGAGCTATTATGTACCCGACTATAAGCTCTGGAGAAACCAAAGGCTTGAACAGGGATGATATTCAAGGAATTAAAGCATTATACCACGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

311

Amino Acids

34.39

Weight (kDa)

9.53

Isoelectric Point (pI)

36.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 371
AccB1I GGYRCC 2 cut(s) 371, 518
AccB7I CCANNNNNTGG 2 cut(s) 347, 671
AccII CGCG 1 cut(s) 704
AciI CCGC 2 cut(s) 413, 530
AclWI GGATC 3 cut(s) 627, 679, 692
AcoI YGGCCR 4 cut(s) 258, 476, 531, 823
AcsI RAATTY 1 cut(s) 76
AcvI CACGTG 1 cut(s) 551
AcyI GRCGYC 2 cut(s) 254, 351
AdeI CACNNNGTG 1 cut(s) 347
AfaI GTAC 3 cut(s) 373, 491, 854
AfiI CCNNNNNNNGG 5 cut(s) 347, 427, 529, 671, 713
AflII CTTAAG 2 cut(s) 131, 205
AgsI TTSAA 4 cut(s) 125, 586, 889, 905
AjnI CCWGG 4 cut(s) 160, 460, 594, 676
AluBI AGCT 3 cut(s) 831, 845, 867
AluI AGCT 3 cut(s) 831, 845, 867
Alw26I GTCTC 3 cut(s) 18, 29, 776
AlwI GGATC 3 cut(s) 627, 679, 692
AoxI GGCC 6 cut(s) 258, 476, 531, 538, 673, 823
ApaI GGGCCC 1 cut(s) 542
ApeKI GCWGC 2 cut(s) 210, 569
ApoI RAATTY 1 cut(s) 76
ArsI GACNNNNNNTTYG 2 cut(s) 775, 807
Asp718I GGTACC 1 cut(s) 371
AspA2I CCTAGG 1 cut(s) 814
AspLEI GCGC 2 cut(s) 694, 706
AspS9I GGNCC 4 cut(s) 157, 538, 539, 674
AsuHPI GGTGA 6 cut(s) 99, 161, 395, 490, 629, 800
AvaII GGWCC 1 cut(s) 157
AvrII CCTAGG 1 cut(s) 814
BaeGI GKGCMC 2 cut(s) 521, 542
BalI TGGCCA 2 cut(s) 478, 825
BamHI GGATCC 1 cut(s) 684
BanI GGYRCC 2 cut(s) 371, 518
BanII GRGCYC 1 cut(s) 542
BbrPI CACGTG 1 cut(s) 551
BbvI GCAGC 2 cut(s) 222, 556
BccI CCATC 5 cut(s) 269, 429, 650, 665, 707
BceAI ACGGC 1 cut(s) 543
BciT130I CCWGG 4 cut(s) 162, 462, 596, 678
BciVI GTATCC 1 cut(s) 334
BcoDI GTCTC 3 cut(s) 18, 29, 776
BfaI CTAG 2 cut(s) 689, 815
BfrI CTTAAG 2 cut(s) 131, 205
BfuI GTATCC 1 cut(s) 334
BglI GCCNNNNNGGC 1 cut(s) 475
BisI GCNGC 3 cut(s) 211, 531, 570
BlnI CCTAGG 1 cut(s) 814
BlsI GCNGC 3 cut(s) 212, 532, 571
Bme1390I CCNGG 4 cut(s) 162, 462, 596, 678
Bme18I GGWCC 1 cut(s) 157
BmgT120I GGNCC 4 cut(s) 157, 538, 539, 674
BmiI GGNNCC 6 cut(s) 140, 159, 373, 520, 540, 686
BmrFI CCNGG 4 cut(s) 162, 462, 596, 678
BmrI ACTGGG 1 cut(s) 536
BmsI GCATC 3 cut(s) 14, 327, 670
BmuI ACTGGG 1 cut(s) 536
BpmI CTGGAG 1 cut(s) 891
BsaAI YACGTR 2 cut(s) 551, 564
BsaBI GATNNNNATC 1 cut(s) 660
BsaHI GRCGYC 2 cut(s) 254, 351
BsaJI CCNNGG 7 cut(s) 160, 187, 460, 534, 649, 676, 814
Bsc4I CCNNNNNNNGG 5 cut(s) 347, 427, 529, 671, 713
Bse118I RCCGGY 1 cut(s) 639
Bse1I ACTGG 2 cut(s) 304, 542
Bse8I GATNNNNATC 1 cut(s) 660
BseBI CCWGG 4 cut(s) 162, 462, 596, 678
BseDI CCNNGG 7 cut(s) 160, 187, 460, 534, 649, 676, 814
BseGI GGATG 4 cut(s) 48, 80, 661, 901
BseJI GATNNNNATC 1 cut(s) 660
BseLI CCNNNNNNNGG 5 cut(s) 347, 427, 529, 671, 713
BseNI ACTGG 2 cut(s) 304, 542
BseRI GAGGAG 3 cut(s) 32, 367, 399
BseSI GKGCMC 2 cut(s) 521, 542
BseXI GCAGC 2 cut(s) 222, 556
Bsh1236I CGCG 1 cut(s) 704
BshFI GGCC 6 cut(s) 260, 478, 533, 540, 675, 825
BshNI GGYRCC 2 cut(s) 371, 518
BsiSI CCGG 2 cut(s) 617, 640
BslFI GGGAC 3 cut(s) 143, 338, 444
BslI CCNNNNNNNGG 5 cut(s) 347, 427, 529, 671, 713
BsmAI GTCTC 3 cut(s) 18, 29, 776
BsmFI GGGAC 3 cut(s) 143, 338, 444
BsmI GAATGC 1 cut(s) 694
BsnI GGCC 6 cut(s) 260, 478, 533, 540, 675, 825
Bsp120I GGGCCC 1 cut(s) 538
Bsp1286I GDGCHC 2 cut(s) 521, 542
Bsp143I GATC 4 cut(s) 244, 386, 619, 684
Bsp19I CCATGG 1 cut(s) 649
BspACI CCGC 2 cut(s) 413, 530
BspANI GGCC 6 cut(s) 260, 478, 533, 540, 675, 825
BspFNI CGCG 1 cut(s) 704
BspLI GGNNCC 6 cut(s) 140, 159, 373, 520, 540, 686
BspPI GGATC 3 cut(s) 627, 679, 692
BspT107I GGYRCC 2 cut(s) 371, 518
BspTI CTTAAG 2 cut(s) 131, 205
BsrFI RCCGGY 1 cut(s) 639
BsrI ACTGG 2 cut(s) 304, 542
BssAI RCCGGY 1 cut(s) 639
BssECI CCNNGG 7 cut(s) 160, 187, 460, 534, 649, 676, 814
BssMI GATC 4 cut(s) 244, 386, 619, 684
BssNI GRCGYC 2 cut(s) 254, 351
BssT1I CCWWGG 3 cut(s) 534, 649, 814
Bst2UI CCWGG 4 cut(s) 162, 462, 596, 678
Bst4CI ACNGT 5 cut(s) 322, 439, 582, 628, 787
Bst6I CTCTTC 1 cut(s) 50
BstACI GRCGYC 2 cut(s) 254, 351
BstAFI CTTAAG 2 cut(s) 131, 205
BstBAI YACGTR 2 cut(s) 551, 564
BstC8I GCNNGC 3 cut(s) 34, 395, 480
BstDEI CTNAG 1 cut(s) 486
BstDSI CCRYGG 1 cut(s) 649
BstEII GGTNACC 1 cut(s) 806
BstF5I GGATG 4 cut(s) 48, 80, 661, 901
BstFNI CGCG 1 cut(s) 704
BstHHI GCGC 2 cut(s) 694, 706
BstKTI GATC 4 cut(s) 247, 389, 622, 687
BstMAI GTCTC 3 cut(s) 18, 29, 776
BstMBI GATC 4 cut(s) 244, 386, 619, 684
BstMWI GCNNNNNNNGC 1 cut(s) 475
BstNI CCWGG 4 cut(s) 162, 462, 596, 678
BstNSI RCATGY 1 cut(s) 36
BstPI GGTNACC 1 cut(s) 806
BstSCI CCNGG 4 cut(s) 160, 460, 594, 676
BstSLI GKGCMC 2 cut(s) 521, 542
BstUI CGCG 1 cut(s) 704
BstV1I GCAGC 2 cut(s) 222, 556
BstX2I RGATCY 2 cut(s) 619, 684
BstXI CCANNNNNNTGG 1 cut(s) 656
BstYI RGATCY 2 cut(s) 619, 684
BsuI GTATCC 1 cut(s) 334
BsuRI GGCC 6 cut(s) 260, 478, 533, 540, 675, 825
BtgI CCRYGG 1 cut(s) 649
BtsCI GGATG 4 cut(s) 48, 80, 661, 901
BtsIMutI CAGTG 2 cut(s) 228, 297
Cac8I GCNNGC 3 cut(s) 34, 395, 480
CfoI GCGC 2 cut(s) 694, 706
Cfr10I RCCGGY 1 cut(s) 639
Cfr13I GGNCC 4 cut(s) 157, 538, 539, 674
CseI GACGC 4 cut(s) 243, 320, 340, 740
Csp6I GTAC 3 cut(s) 372, 490, 853
CviAII CATG 4 cut(s) 33, 71, 650, 797
CviQI GTAC 3 cut(s) 372, 490, 853
DdeI CTNAG 1 cut(s) 486
DpnI GATC 4 cut(s) 246, 388, 621, 686
DpnII GATC 4 cut(s) 244, 386, 619, 684
DraIII CACNNNGTG 1 cut(s) 347
EaeI YGGCCR 4 cut(s) 258, 476, 531, 823
Eam1104I CTCTTC 1 cut(s) 50
EarI CTCTTC 1 cut(s) 50
Eco130I CCWWGG 3 cut(s) 534, 649, 814
Eco24I GRGCYC 1 cut(s) 542
Eco32I GATATC 1 cut(s) 361
Eco47I GGWCC 1 cut(s) 157
Eco72I CACGTG 1 cut(s) 551
Eco91I GGTNACC 1 cut(s) 806
EcoO109I RGGNCCY 2 cut(s) 157, 538
EcoO65I GGTNACC 1 cut(s) 806
EcoRII CCWGG 4 cut(s) 160, 460, 594, 676
EcoRV GATATC 1 cut(s) 361
EcoT14I CCWWGG 3 cut(s) 534, 649, 814
EcoT38I GRGCYC 1 cut(s) 542
ErhI CCWWGG 3 cut(s) 534, 649, 814
FaeI CATG 4 cut(s) 36, 74, 653, 800
FaqI GGGAC 3 cut(s) 143, 338, 444
FatI CATG 4 cut(s) 32, 70, 649, 796
Fnu4HI GCNGC 3 cut(s) 211, 531, 570
FokI GGATG 4 cut(s) 35, 67, 648, 908
FriOI GRGCYC 1 cut(s) 542
Fsp4HI GCNGC 3 cut(s) 211, 531, 570
FspBI CTAG 2 cut(s) 689, 815
GlaI GCGC 2 cut(s) 693, 705
GluI GCNGC 3 cut(s) 211, 531, 570
GsuI CTGGAG 1 cut(s) 891
HaeIII GGCC 6 cut(s) 260, 478, 533, 540, 675, 825
HapII CCGG 2 cut(s) 617, 640
HgaI GACGC 4 cut(s) 243, 320, 340, 740
HhaI GCGC 2 cut(s) 694, 706
Hin1I GRCGYC 2 cut(s) 254, 351
Hin1II CATG 4 cut(s) 36, 74, 653, 800
Hin6I GCGC 2 cut(s) 692, 704
HinP1I GCGC 2 cut(s) 692, 704
HinfI GANTC 2 cut(s) 165, 607
HpaII CCGG 2 cut(s) 617, 640
HphI GGTGA 6 cut(s) 99, 161, 395, 490, 629, 800
Hpy188I TCNGA 5 cut(s) 85, 191, 612, 624, 840
Hpy188III TCNNGA 1 cut(s) 870
Hpy99I CGWCG 2 cut(s) 259, 529
HpyAV CCTTC 2 cut(s) 186, 597
HpyCH4III ACNGT 5 cut(s) 322, 439, 582, 628, 787
HpyCH4IV ACGT 3 cut(s) 343, 550, 563
HpyCH4V TGCA 4 cut(s) 36, 74, 548, 796
HpyF10VI GCNNNNNNNGC 1 cut(s) 475
HpyF3I CTNAG 1 cut(s) 486
HpySE526I ACGT 3 cut(s) 343, 550, 563
Hsp92I GRCGYC 2 cut(s) 254, 351
Hsp92II CATG 4 cut(s) 36, 74, 653, 800
HspAI GCGC 2 cut(s) 692, 704
KpnI GGTACC 1 cut(s) 375
Kzo9I GATC 4 cut(s) 244, 386, 619, 684
Lsp1109I GCAGC 2 cut(s) 222, 556
LweI GCATC 3 cut(s) 14, 327, 670
MaeI CTAG 2 cut(s) 689, 815
MaeII ACGT 3 cut(s) 343, 550, 563
MaeIII GTNAC 4 cut(s) 149, 200, 401, 806
MalI GATC 4 cut(s) 246, 388, 621, 686
MboI GATC 4 cut(s) 244, 386, 619, 684
MboII GAAGA 3 cut(s) 37, 448, 503
MfeI CAATTG 1 cut(s) 248
MflI RGATCY 2 cut(s) 619, 684
MhlI GDGCHC 2 cut(s) 521, 542
MlsI TGGCCA 2 cut(s) 478, 825
MluCI AATT 5 cut(s) 76, 248, 280, 801, 909
MluNI TGGCCA 2 cut(s) 478, 825
Mox20I TGGCCA 2 cut(s) 478, 825
MscI TGGCCA 2 cut(s) 478, 825
MseI TTAA 4 cut(s) 132, 206, 558, 912
MslI CAYNNNNRTG 1 cut(s) 654
Msp20I TGGCCA 2 cut(s) 478, 825
MspCI CTTAAG 2 cut(s) 131, 205
MspI CCGG 2 cut(s) 617, 640
MspR9I CCNGG 4 cut(s) 162, 462, 596, 678
MunI CAATTG 1 cut(s) 248
Mva1269I GAATGC 1 cut(s) 694
MvaI CCWGG 4 cut(s) 162, 462, 596, 678
MvnI CGCG 1 cut(s) 704
MwoI GCNNNNNNNGC 1 cut(s) 475
NcoI CCATGG 1 cut(s) 649
NdeII GATC 4 cut(s) 244, 386, 619, 684
NlaIII CATG 4 cut(s) 36, 74, 653, 800
NlaIV GGNNCC 6 cut(s) 140, 159, 373, 520, 540, 686
NmuCI GTSAC 3 cut(s) 149, 401, 806
NspI RCATGY 1 cut(s) 36
PaeI GCATGC 1 cut(s) 36
PctI GAATGC 1 cut(s) 694
PfeI GAWTC 2 cut(s) 165, 607
PflFI GACNNNGTC 1 cut(s) 155
PflMI CCANNNNNTGG 2 cut(s) 347, 671
PkrI GCNGC 3 cut(s) 212, 532, 571
PmaCI CACGTG 1 cut(s) 551
PmlI CACGTG 1 cut(s) 551
Ppu21I YACGTR 2 cut(s) 551, 564
PpuMI RGGWCCY 1 cut(s) 157
Psp5II RGGWCCY 1 cut(s) 157
Psp6I CCWGG 4 cut(s) 160, 460, 594, 676
PspCI CACGTG 1 cut(s) 551
PspEI GGTNACC 1 cut(s) 806
PspGI CCWGG 4 cut(s) 160, 460, 594, 676
PspN4I GGNNCC 6 cut(s) 140, 159, 373, 520, 540, 686
PspOMI GGGCCC 1 cut(s) 538
PspPI GGNCC 4 cut(s) 157, 538, 539, 674
PspPPI RGGWCCY 1 cut(s) 157
PsuI RGATCY 2 cut(s) 619, 684
PsyI GACNNNGTC 1 cut(s) 155
RsaI GTAC 3 cut(s) 373, 491, 854
RsaNI GTAC 3 cut(s) 372, 490, 853
RseI CAYNNNNRTG 1 cut(s) 654
SaqAI TTAA 4 cut(s) 132, 206, 558, 912
SatI GCNGC 3 cut(s) 211, 531, 570
Sau3AI GATC 4 cut(s) 244, 386, 619, 684
Sau96I GGNCC 4 cut(s) 157, 538, 539, 674
ScrFI CCNGG 4 cut(s) 162, 462, 596, 678
SduI GDGCHC 2 cut(s) 521, 542
SfaNI GCATC 3 cut(s) 14, 327, 670
SgrAI CRCCGGYG 1 cut(s) 639
SinI GGWCC 1 cut(s) 157
SmiMI CAYNNNNRTG 1 cut(s) 654
SmlI CTYRAG 2 cut(s) 131, 205
SmoI CTYRAG 2 cut(s) 131, 205
SphI GCATGC 1 cut(s) 36
Sse9I AATT 5 cut(s) 76, 248, 280, 801, 909
SsiI CCGC 2 cut(s) 413, 530
SspI AATATT 1 cut(s) 306
SspMI CTAG 2 cut(s) 689, 815
StyD4I CCNGG 4 cut(s) 160, 460, 594, 676
StyI CCWWGG 3 cut(s) 534, 649, 814
TaaI ACNGT 5 cut(s) 322, 439, 582, 628, 787
TaiI ACGT 3 cut(s) 346, 553, 566
TaqI TCGA 1 cut(s) 601
TasI AATT 5 cut(s) 76, 248, 280, 801, 909
TauI GCSGC 1 cut(s) 533
TfiI GAWTC 2 cut(s) 165, 607
Tru1I TTAA 4 cut(s) 132, 206, 558, 912
Tru9I TTAA 4 cut(s) 132, 206, 558, 912
TscAI CASTG 2 cut(s) 235, 304
TseFI GTSAC 3 cut(s) 149, 401, 806
TseI GCWGC 2 cut(s) 210, 569
Tsp45I GTSAC 3 cut(s) 149, 401, 806
TspDTI ATGAA 3 cut(s) 37, 69, 813
TspRI CASTG 2 cut(s) 235, 304
Tth111I GACNNNGTC 1 cut(s) 155
Van91I CCANNNNNTGG 2 cut(s) 347, 671
Vha464I CTTAAG 2 cut(s) 131, 205
VpaK11BI GGWCC 1 cut(s) 157
XapI RAATTY 1 cut(s) 76
XceI RCATGY 1 cut(s) 36
XcmI CCANNNNNNNNNTGG 1 cut(s) 304
XmaJI CCTAGG 1 cut(s) 814
XspI CTAG 2 cut(s) 689, 815
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.