Prupe.3G277600_v2.0.a1

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
N/A
Physical Location & Seq
Reverse (-)
25502315 .. 25503363
1049 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G277600.1_v2.0.a1.CDS.1

Sequence Viewer

Length: 828 bp
ATGGAAGGCCTAACTCTCAATCTCAATGTGGAAGTTAGAGTTGTTAAGGGGTGTCGCAAGGGTGACAATGTCCAAGGCATCCTAGACGTCAAAAAACACCTTGAAAAATTTGGTTACTTGAGTGGCCATTCCCAGAATGATGATTTTGACGACCAATTGGAGTCGGCCATCAAAACTTACCAAATCAATTACAACCTCGAGGCCACCGGAAGGTTGGATGCCAAAACTGTGTCAAACATGATGATACCACGTTGTGGTGTGCCAGATATCATCAATGGTACCACCTCCATGATATCAAGAAAGATACAGCACCACGAGGGGCTCACAGTTTCTCAATATGCTTTCTTCCCAGGAAACCCAAAATGGCCTTCGTCTAAGTATCGCCTCACCTATGCTTTTCTTCAGGGCACCCCAAATCAGGCCAAAGATCCGGTTAAACGTGCTTTTCAAACATGGGCCAATAACACACAGTTTACGTTCAGTCAGGTCGAAGAATATGGGACTCCAGATCTGACGATCAGTTTTCAGAGGCGCGATCATGGAGATGGGTATCCGTTTGATGGCCGAGGTGGGATAACCGCCCATGCTTTTTCGCCAACAGATGGGAGATTTCACTACGACGCTGATGAGATGTGGGTTGTGGGTTCTGTGAATGGTGGCATGGACTTGCAGAGTGTTGCTGTGCATGAAATAGGGCACCTTCTTGGGCTTAATCATAGCTTTGTTGAAGACGCTATTATGTACCCCTATCTCAACACTGCAACGGTCAAGCGGAATTTGAAGCCCGACGATATTCAAGGAATTAAAAACTTATACAACATTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

276

Amino Acids

30.9

Weight (kDa)

6.36

Isoelectric Point (pI)

26.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 90
AbsI CCTCGAGG 1 cut(s) 197
Acc65I GGTACC 1 cut(s) 278
AccB1I GGYRCC 3 cut(s) 278, 407, 696
AccB7I CCANNNNNTGG 2 cut(s) 254, 602
AccII CGCG 1 cut(s) 534
AciI CCGC 2 cut(s) 579, 772
AclWI GGATC 1 cut(s) 422
AcoI YGGCCR 3 cut(s) 124, 165, 562
AcsI RAATTY 2 cut(s) 107, 775
AcuI CTGAAG 1 cut(s) 386
AcyI GRCGYC 1 cut(s) 87
AdeI CACNNNGTG 1 cut(s) 254
AfaI GTAC 2 cut(s) 280, 743
AfiI CCNNNNNNNGG 5 cut(s) 210, 254, 418, 560, 602
AgsI TTSAA 5 cut(s) 104, 449, 728, 781, 797
AjnI CCWGG 1 cut(s) 349
AjuI GAANNNNNNNTTGG 2 cut(s) 352, 384
AluBI AGCT 1 cut(s) 720
AluI AGCT 1 cut(s) 720
AlwI GGATC 1 cut(s) 422
Ama87I CYCGRG 1 cut(s) 197
AoxI GGCC 8 cut(s) 7, 124, 165, 201, 365, 420, 456, 562
ApoI RAATTY 2 cut(s) 107, 775
Asp718I GGTACC 1 cut(s) 278
AspLEI GCGC 1 cut(s) 534
AspS9I GGNCC 1 cut(s) 456
AsuHPI GGTGA 2 cut(s) 74, 379
AvaI CYCGRG 1 cut(s) 197
BaeGI GKGCMC 2 cut(s) 410, 699
BalI TGGCCA 1 cut(s) 126
BanI GGYRCC 3 cut(s) 278, 407, 696
BanII GRGCYC 1 cut(s) 324
BauI CACGAG 1 cut(s) 314
BbsI GAAGAC 1 cut(s) 735
BccI CCATC 4 cut(s) 176, 539, 554, 596
BciT130I CCWGG 1 cut(s) 351
BciVI GTATCC 1 cut(s) 561
BfaI CTAG 1 cut(s) 83
BfuI GTATCC 1 cut(s) 561
BglII AGATCT 1 cut(s) 508
Bme1390I CCNGG 1 cut(s) 351
BmeT110I CYCGRG 1 cut(s) 197
BmgT120I GGNCC 1 cut(s) 456
BmiI GGNNCC 3 cut(s) 280, 409, 698
BmrFI CCNGG 1 cut(s) 351
BmsI GCATC 2 cut(s) 87, 208
BpiI GAAGAC 1 cut(s) 735
BpmI CTGGAG 1 cut(s) 489
BpuEI CTTGAG 1 cut(s) 139
BsaBI GATNNNNATC 1 cut(s) 549
BsaHI GRCGYC 1 cut(s) 87
BsaJI CCNNGG 3 cut(s) 73, 349, 565
BsaWI WCCGGW 2 cut(s) 206, 430
Bsc4I CCNNNNNNNGG 5 cut(s) 210, 254, 418, 560, 602
Bse8I GATNNNNATC 1 cut(s) 549
BseBI CCWGG 1 cut(s) 351
BseDI CCNNGG 3 cut(s) 73, 349, 565
BseGI GGATG 2 cut(s) 78, 223
BseJI GATNNNNATC 1 cut(s) 549
BseLI CCNNNNNNNGG 5 cut(s) 210, 254, 418, 560, 602
BseSI GKGCMC 2 cut(s) 410, 699
Bsh1236I CGCG 1 cut(s) 534
BshFI GGCC 8 cut(s) 9, 126, 167, 203, 367, 422, 458, 564
BshNI GGYRCC 3 cut(s) 278, 407, 696
BsiHKCI CYCGRG 1 cut(s) 197
BsiSI CCGG 2 cut(s) 207, 431
BslFI GGGAC 1 cut(s) 514
BslI CCNNNNNNNGG 5 cut(s) 210, 254, 418, 560, 602
BsmFI GGGAC 1 cut(s) 514
BsnI GGCC 8 cut(s) 9, 126, 167, 203, 367, 422, 458, 564
BsoBI CYCGRG 1 cut(s) 197
Bsp1286I GDGCHC 3 cut(s) 324, 410, 699
Bsp143I GATC 4 cut(s) 427, 508, 516, 535
BspACI CCGC 2 cut(s) 579, 772
BspANI GGCC 8 cut(s) 9, 126, 167, 203, 367, 422, 458, 564
BspFNI CGCG 1 cut(s) 534
BspLI GGNNCC 3 cut(s) 280, 409, 698
BspPI GGATC 1 cut(s) 422
BspT107I GGYRCC 3 cut(s) 278, 407, 696
BssECI CCNNGG 3 cut(s) 73, 349, 565
BssMI GATC 4 cut(s) 427, 508, 516, 535
BssNI GRCGYC 1 cut(s) 87
BssSI CACGAG 1 cut(s) 314
BssT1I CCWWGG 1 cut(s) 73
Bst2BI CACGAG 1 cut(s) 314
Bst2UI CCWGG 1 cut(s) 351
Bst4CI ACNGT 4 cut(s) 229, 328, 471, 766
BstACI GRCGYC 1 cut(s) 87
BstDEI CTNAG 1 cut(s) 375
BstF5I GGATG 2 cut(s) 78, 223
BstFNI CGCG 1 cut(s) 534
BstHHI GCGC 1 cut(s) 534
BstKTI GATC 4 cut(s) 430, 511, 519, 538
BstMBI GATC 4 cut(s) 427, 508, 516, 535
BstNI CCWGG 1 cut(s) 351
BstSCI CCNGG 1 cut(s) 349
BstSLI GKGCMC 2 cut(s) 410, 699
BstUI CGCG 1 cut(s) 534
BstV2I GAAGAC 1 cut(s) 735
BstX2I RGATCY 2 cut(s) 427, 508
BstYI RGATCY 2 cut(s) 427, 508
BsuI GTATCC 1 cut(s) 561
BsuRI GGCC 8 cut(s) 9, 126, 167, 203, 367, 422, 458, 564
BtsCI GGATG 2 cut(s) 78, 223
BtsI GCAGTG 1 cut(s) 756
BtsIMutI CAGTG 1 cut(s) 756
CfoI GCGC 1 cut(s) 534
Cfr13I GGNCC 1 cut(s) 456
CseI GACGC 2 cut(s) 629, 740
Csp6I GTAC 2 cut(s) 279, 742
CviAII CATG 7 cut(s) 238, 289, 453, 539, 584, 661, 686
CviQI GTAC 2 cut(s) 279, 742
DdeI CTNAG 1 cut(s) 375
DpnI GATC 4 cut(s) 429, 510, 518, 537
DpnII GATC 4 cut(s) 427, 508, 516, 535
DraIII CACNNNGTG 1 cut(s) 254
EaeI YGGCCR 3 cut(s) 124, 165, 562
Eco130I CCWWGG 1 cut(s) 73
Eco147I AGGCCT 1 cut(s) 9
Eco24I GRGCYC 1 cut(s) 324
Eco32I GATATC 2 cut(s) 268, 294
Eco57I CTGAAG 1 cut(s) 386
Eco88I CYCGRG 1 cut(s) 197
EcoRII CCWGG 1 cut(s) 349
EcoRV GATATC 2 cut(s) 268, 294
EcoT14I CCWWGG 1 cut(s) 73
EcoT38I GRGCYC 1 cut(s) 324
ErhI CCWWGG 1 cut(s) 73
FaeI CATG 7 cut(s) 241, 292, 456, 542, 587, 664, 689
FaqI GGGAC 1 cut(s) 514
FatI CATG 7 cut(s) 237, 288, 452, 538, 583, 660, 685
FokI GGATG 2 cut(s) 65, 230
FriOI GRGCYC 1 cut(s) 324
FspBI CTAG 1 cut(s) 83
GlaI GCGC 1 cut(s) 533
GsuI CTGGAG 1 cut(s) 489
HaeIII GGCC 8 cut(s) 9, 126, 167, 203, 367, 422, 458, 564
HapII CCGG 2 cut(s) 207, 431
HgaI GACGC 2 cut(s) 629, 740
HhaI GCGC 1 cut(s) 534
Hin1I GRCGYC 1 cut(s) 87
Hin1II CATG 7 cut(s) 241, 292, 456, 542, 587, 664, 689
Hin6I GCGC 1 cut(s) 532
HinP1I GCGC 1 cut(s) 532
HinfI GANTC 2 cut(s) 161, 502
HpaII CCGG 2 cut(s) 207, 431
HphI GGTGA 2 cut(s) 74, 379
Hpy166II GTNNAC 1 cut(s) 474
Hpy188I TCNGA 2 cut(s) 513, 528
Hpy188III TCNNGA 2 cut(s) 297, 506
Hpy8I GTNNAC 1 cut(s) 474
Hpy99I CGWCG 2 cut(s) 623, 791
HpyAV CCTTC 3 cut(s) 204, 378, 710
HpyCH4III ACNGT 4 cut(s) 229, 328, 471, 766
HpyCH4IV ACGT 4 cut(s) 87, 250, 439, 476
HpyCH4V TGCA 3 cut(s) 670, 685, 761
HpyF3I CTNAG 1 cut(s) 375
HpySE526I ACGT 4 cut(s) 87, 250, 439, 476
Hsp92I GRCGYC 1 cut(s) 87
Hsp92II CATG 7 cut(s) 241, 292, 456, 542, 587, 664, 689
HspAI GCGC 1 cut(s) 532
KpnI GGTACC 1 cut(s) 282
Kzo9I GATC 4 cut(s) 427, 508, 516, 535
LweI GCATC 2 cut(s) 87, 208
MaeI CTAG 1 cut(s) 83
MaeII ACGT 4 cut(s) 87, 250, 439, 476
MaeIII GTNAC 2 cut(s) 62, 113
MalI GATC 4 cut(s) 429, 510, 518, 537
MboI GATC 4 cut(s) 427, 508, 516, 535
MboII GAAGA 4 cut(s) 337, 392, 503, 740
MfeI CAATTG 1 cut(s) 155
MflI RGATCY 2 cut(s) 427, 508
MhlI GDGCHC 3 cut(s) 324, 410, 699
MlsI TGGCCA 1 cut(s) 126
MluCI AATT 5 cut(s) 107, 155, 187, 775, 801
MluNI TGGCCA 1 cut(s) 126
MlyI GAGTC 2 cut(s) 170, 496
MmeI TCCRAC 1 cut(s) 195
MnlI CCTC 7 cut(s) 193, 206, 295, 310, 395, 522, 560
Mox20I TGGCCA 1 cut(s) 126
MscI TGGCCA 1 cut(s) 126
MseI TTAA 5 cut(s) 45, 435, 711, 804, 822
MslI CAYNNNNRTG 2 cut(s) 287, 543
Msp20I TGGCCA 1 cut(s) 126
MspI CCGG 2 cut(s) 207, 431
MspR9I CCNGG 1 cut(s) 351
MunI CAATTG 1 cut(s) 155
MvaI CCWGG 1 cut(s) 351
MvnI CGCG 1 cut(s) 534
NdeII GATC 4 cut(s) 427, 508, 516, 535
NlaIII CATG 7 cut(s) 241, 292, 456, 542, 587, 664, 689
NlaIV GGNNCC 3 cut(s) 280, 409, 698
NmeAIII GCCGAG 1 cut(s) 590
NmuCI GTSAC 1 cut(s) 62
PaeR7I CTCGAG 1 cut(s) 197
PceI AGGCCT 1 cut(s) 9
PflFI GACNNNGTC 1 cut(s) 68
PflMI CCANNNNNTGG 2 cut(s) 254, 602
PleI GAGTC 2 cut(s) 169, 496
PpsI GAGTC 2 cut(s) 169, 496
Psp6I CCWGG 1 cut(s) 349
PspGI CCWGG 1 cut(s) 349
PspN4I GGNNCC 3 cut(s) 280, 409, 698
PspPI GGNCC 1 cut(s) 456
PspXI VCTCGAGB 1 cut(s) 197
PsuI RGATCY 2 cut(s) 427, 508
PsyI GACNNNGTC 1 cut(s) 68
RsaI GTAC 2 cut(s) 280, 743
RsaNI GTAC 2 cut(s) 279, 742
RseI CAYNNNNRTG 2 cut(s) 287, 543
SaqAI TTAA 5 cut(s) 45, 435, 711, 804, 822
Sau3AI GATC 4 cut(s) 427, 508, 516, 535
Sau96I GGNCC 1 cut(s) 456
SchI GAGTC 2 cut(s) 170, 496
ScrFI CCNGG 1 cut(s) 351
SduI GDGCHC 3 cut(s) 324, 410, 699
SfaNI GCATC 2 cut(s) 87, 208
Sfr274I CTCGAG 1 cut(s) 197
SlaI CTCGAG 1 cut(s) 197
SmiMI CAYNNNNRTG 2 cut(s) 287, 543
SmlI CTYRAG 2 cut(s) 118, 197
SmoI CTYRAG 2 cut(s) 118, 197
Sse9I AATT 5 cut(s) 107, 155, 187, 775, 801
SseBI AGGCCT 1 cut(s) 9
SsiI CCGC 2 cut(s) 579, 772
SspMI CTAG 1 cut(s) 83
StuI AGGCCT 1 cut(s) 9
StyD4I CCNGG 1 cut(s) 349
StyI CCWWGG 1 cut(s) 73
TaaI ACNGT 4 cut(s) 229, 328, 471, 766
TaiI ACGT 4 cut(s) 90, 253, 442, 479
TaqI TCGA 2 cut(s) 198, 489
TasI AATT 5 cut(s) 107, 155, 187, 775, 801
Tru1I TTAA 5 cut(s) 45, 435, 711, 804, 822
Tru9I TTAA 5 cut(s) 45, 435, 711, 804, 822
TscAI CASTG 1 cut(s) 763
TseFI GTSAC 1 cut(s) 62
Tsp45I GTSAC 1 cut(s) 62
TspDTI ATGAA 1 cut(s) 702
TspGWI ACGGA 1 cut(s) 543
TspRI CASTG 1 cut(s) 763
Tth111I GACNNNGTC 1 cut(s) 68
Van91I CCANNNNNTGG 2 cut(s) 254, 602
XapI RAATTY 2 cut(s) 107, 775
XcmI CCANNNNNNNNNTGG 1 cut(s) 211
XhoI CTCGAG 1 cut(s) 197
XspI CTAG 1 cut(s) 83
ZraI GACGTC 1 cut(s) 88
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.