Prupe.3G277900_v2.0.a1

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
N/A
Physical Location & Seq
Reverse (-)
25515175 .. 25516110
936 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G277900.1_v2.0.a1.CDS.1

Sequence Viewer

Length: 936 bp
ATGGCATCATCCAAAACTTCAACTCCTTCTCTCTTCGCAATCACTACCCTACTCCTCTTCATCCTCCTTTCTCTCCTCTCCTACGCAAAAGCCTCAGAAACTCACCACAAAAAAACATCTTCTTATGAGTTCCTTGAACATCTCAAAGGGTGTCACAAGGGTGACAAAGTTCAAGGCATCCAAGACCTCAAAAAATACCTTGAAAAGTTTGGTTACTTGAATGGCAATACCAACAATGATGACCACTTTGATGACGAACTGGAGTCGGCCATCAAAACTTACCAAATCAATTACCACCTCAAGGTCACTGGAACATTAGATGCGAAAACCGTAAAAAAAATGGAGATGCCTCGATGTGGTGTGCCGGATATCATCAACGGTACCACCTCCATGAGAACAGGAAAGAAAAGGGGAGGACATGGTTCAACTCACACAGTTGGTCATTACGCTTTCTTCCAAGGAAACCCAAAATGGCCTGCCAATAAGTATCACCTCACCTATGGTTTTCTTCAAGGCACCCAATCTGAGGCTGTGGGCGCAGTTGCACGTGCTTTTGCAACATGGCAGAGCAACACACACTTCACGTTCAGCCAAGCCCAAAGCTTTGAGAGCGCTGATCTGAAGATCGGTTTTGGGAGGGGTGATCATGGAGATGGGGCTAATAATGCATTTGATGGCCCAGGTAAGACCATAGCCCATGCTTTTCGGCCGACTAATGGGAGATTTCACTACGATGCTGATGAGACGTGGGTGGTGGGCGCTGTGCCGGGTGGTTTCGACTTGGAGACTGTGGCTTTGCACGAAATTGGACACCTTCTTGGACTTGACCATAGCTCTGTTCCAGGAGCTGTCATGCAATCTCAAATTCCTCCGGGATATACCCAAAGTTTGCATGCGGATGATGTTCAAGGAATTAGAGCTTTATACAACACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

312

Amino Acids

34.05

Weight (kDa)

7.02

Isoelectric Point (pI)

26.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 380
AccB1I GGYRCC 2 cut(s) 380, 515
AciI CCGC 1 cut(s) 896
AcoI YGGCCR 2 cut(s) 267, 707
AcsI RAATTY 1 cut(s) 864
AcuI CTGAAG 1 cut(s) 641
AcvI CACGTG 1 cut(s) 548
AfaI GTAC 1 cut(s) 382
AfeI AGCGCT 1 cut(s) 613
AfiI CCNNNNNNNGG 4 cut(s) 301, 356, 526, 716
AgsI TTSAA 8 cut(s) 21, 137, 173, 203, 220, 426, 512, 908
AjiI CACGTC 1 cut(s) 747
AjnI CCWGG 2 cut(s) 679, 841
AleI CACNNNNGTG 1 cut(s) 159
AluBI AGCT 4 cut(s) 603, 834, 848, 920
AluI AGCT 4 cut(s) 603, 834, 848, 920
Alw26I GTCTC 2 cut(s) 737, 779
AlwNI CAGNNNCTG 1 cut(s) 848
Aor51HI AGCGCT 1 cut(s) 613
AoxI GGCC 4 cut(s) 267, 473, 676, 707
ApoI RAATTY 1 cut(s) 864
ArsI GACNNNNNNTTYG 2 cut(s) 778, 810
Asp718I GGTACC 1 cut(s) 380
AspLEI GCGC 3 cut(s) 539, 614, 761
AspS9I GGNCC 1 cut(s) 677
AsuC2I CCSGG 2 cut(s) 768, 873
AsuHPI GGTGA 5 cut(s) 95, 173, 482, 487, 653
BanI GGYRCC 2 cut(s) 380, 515
BbrPI CACGTG 1 cut(s) 548
BccI CCATC 3 cut(s) 278, 647, 668
BciT130I CCWGG 2 cut(s) 681, 843
BclI TGATCA 1 cut(s) 643
BcnI CCSGG 2 cut(s) 768, 873
BcoDI GTCTC 2 cut(s) 737, 779
BfoI RGCGCY 2 cut(s) 615, 762
Bme1390I CCNGG 4 cut(s) 681, 768, 843, 873
BmgBI CACGTC 1 cut(s) 747
BmgT120I GGNCC 1 cut(s) 677
BmiI GGNNCC 2 cut(s) 382, 517
BmrFI CCNGG 4 cut(s) 681, 768, 843, 873
BmsI GCATC 5 cut(s) 14, 186, 310, 336, 724
BpmI CTGGAG 1 cut(s) 281
BpuEI CTTGAG 1 cut(s) 284
BpuMI CCSGG 2 cut(s) 768, 873
BsaAI YACGTR 1 cut(s) 548
BsaJI CCNNGG 2 cut(s) 457, 679
BsaXI ACNNNNNCTCC 2 cut(s) 7, 37
Bsc4I CCNNNNNNNGG 4 cut(s) 301, 356, 526, 716
Bse1I ACTGG 2 cut(s) 264, 313
BseBI CCWGG 2 cut(s) 681, 843
BseDI CCNNGG 2 cut(s) 457, 679
BseGI GGATG 4 cut(s) 8, 60, 177, 904
BseLI CCNNNNNNNGG 4 cut(s) 301, 356, 526, 716
BseMII CTCAG 2 cut(s) 108, 516
BseNI ACTGG 2 cut(s) 264, 313
BseRI GAGGAG 2 cut(s) 44, 65
BseX3I CGGCCG 1 cut(s) 707
Bsh1285I CGRYCG 1 cut(s) 710
BshFI GGCC 4 cut(s) 269, 475, 678, 709
BshNI GGYRCC 2 cut(s) 380, 515
BsiEI CGRYCG 1 cut(s) 710
BsiSI CCGG 3 cut(s) 365, 767, 872
BslI CCNNNNNNNGG 4 cut(s) 301, 356, 526, 716
BsmAI GTCTC 2 cut(s) 737, 779
BsmBI CGTCTC 1 cut(s) 737
BsnI GGCC 4 cut(s) 269, 475, 678, 709
Bsp143I GATC 3 cut(s) 616, 624, 643
BspACI CCGC 1 cut(s) 896
BspANI GGCC 4 cut(s) 269, 475, 678, 709
BspCNI CTCAG 2 cut(s) 107, 517
BspLI GGNNCC 2 cut(s) 382, 517
BspT107I GGYRCC 2 cut(s) 380, 515
BsrI ACTGG 2 cut(s) 264, 313
BssECI CCNNGG 2 cut(s) 457, 679
BssMI GATC 3 cut(s) 616, 624, 643
BssT1I CCWWGG 1 cut(s) 457
Bst2UI CCWGG 2 cut(s) 681, 843
Bst4CI ACNGT 4 cut(s) 331, 380, 436, 790
Bst6I CTCTTC 2 cut(s) 38, 62
BstBAI YACGTR 1 cut(s) 548
BstC8I GCNNGC 2 cut(s) 477, 894
BstDEI CTNAG 2 cut(s) 94, 525
BstF5I GGATG 4 cut(s) 8, 60, 177, 904
BstH2I RGCGCY 2 cut(s) 615, 762
BstHHI GCGC 3 cut(s) 539, 614, 761
BstKTI GATC 3 cut(s) 619, 627, 646
BstMAI GTCTC 2 cut(s) 737, 779
BstMBI GATC 3 cut(s) 616, 624, 643
BstMCI CGRYCG 1 cut(s) 710
BstMWI GCNNNNNNNGC 3 cut(s) 536, 609, 665
BstNI CCWGG 2 cut(s) 681, 843
BstNSI RCATGY 1 cut(s) 896
BstSCI CCNGG 4 cut(s) 679, 766, 841, 871
BstZI CGGCCG 1 cut(s) 707
BsuRI GGCC 4 cut(s) 269, 475, 678, 709
BtrI CACGTC 1 cut(s) 747
BtsCI GGATG 4 cut(s) 8, 60, 177, 904
BtsIMutI CAGTG 1 cut(s) 306
Cac8I GCNNGC 2 cut(s) 477, 894
CaiI CAGNNNCTG 1 cut(s) 848
CfoI GCGC 3 cut(s) 539, 614, 761
Cfr13I GGNCC 1 cut(s) 677
Csp6I GTAC 1 cut(s) 381
CviAII CATG 7 cut(s) 391, 419, 561, 647, 698, 853, 893
CviQI GTAC 1 cut(s) 381
DdeI CTNAG 2 cut(s) 94, 525
DpnI GATC 3 cut(s) 618, 626, 645
DpnII GATC 3 cut(s) 616, 624, 643
EaeI YGGCCR 2 cut(s) 267, 707
EagI CGGCCG 1 cut(s) 707
Eam1104I CTCTTC 2 cut(s) 38, 62
EarI CTCTTC 2 cut(s) 38, 62
EclXI CGGCCG 1 cut(s) 707
Eco130I CCWWGG 1 cut(s) 457
Eco32I GATATC 1 cut(s) 370
Eco47III AGCGCT 1 cut(s) 613
Eco52I CGGCCG 1 cut(s) 707
Eco57I CTGAAG 1 cut(s) 641
Eco72I CACGTG 1 cut(s) 548
EcoRII CCWGG 2 cut(s) 679, 841
EcoRV GATATC 1 cut(s) 370
EcoT14I CCWWGG 1 cut(s) 457
EcoT22I ATGCAT 1 cut(s) 670
ErhI CCWWGG 1 cut(s) 457
Esp3I CGTCTC 1 cut(s) 737
FaeI CATG 7 cut(s) 394, 422, 564, 650, 701, 856, 896
FatI CATG 7 cut(s) 390, 418, 560, 646, 697, 852, 892
FbaI TGATCA 1 cut(s) 643
FokI GGATG 3 cut(s) 47, 164, 911
GlaI GCGC 3 cut(s) 538, 613, 760
GsuI CTGGAG 1 cut(s) 281
HaeII RGCGCY 2 cut(s) 615, 762
HaeIII GGCC 4 cut(s) 269, 475, 678, 709
HapII CCGG 3 cut(s) 365, 767, 872
HhaI GCGC 3 cut(s) 539, 614, 761
Hin1II CATG 7 cut(s) 394, 422, 564, 650, 701, 856, 896
Hin6I GCGC 3 cut(s) 537, 612, 759
HinP1I GCGC 3 cut(s) 537, 612, 759
HindIII AAGCTT 1 cut(s) 601
HinfI GANTC 1 cut(s) 263
HpaII CCGG 3 cut(s) 365, 767, 872
HphI GGTGA 5 cut(s) 95, 173, 482, 487, 653
Hpy188I TCNGA 3 cut(s) 97, 526, 621
HpyAV CCTTC 2 cut(s) 36, 824
HpyCH4III ACNGT 4 cut(s) 331, 380, 436, 790
HpyCH4IV ACGT 3 cut(s) 547, 584, 746
HpyCH4V TGCA 6 cut(s) 545, 557, 668, 799, 856, 892
HpyF10VI GCNNNNNNNGC 3 cut(s) 536, 609, 665
HpyF3I CTNAG 2 cut(s) 94, 525
HpySE526I ACGT 3 cut(s) 547, 584, 746
Hsp92II CATG 7 cut(s) 394, 422, 564, 650, 701, 856, 896
HspAI GCGC 3 cut(s) 537, 612, 759
KpnI GGTACC 1 cut(s) 384
Ksp22I TGATCA 1 cut(s) 643
Kzo9I GATC 3 cut(s) 616, 624, 643
LmnI GCTCC 1 cut(s) 845
LweI GCATC 5 cut(s) 14, 186, 310, 336, 724
MaeII ACGT 3 cut(s) 547, 584, 746
MaeIII GTNAC 4 cut(s) 152, 161, 212, 304
MalI GATC 3 cut(s) 618, 626, 645
MboI GATC 3 cut(s) 616, 624, 643
MboII GAAGA 6 cut(s) 25, 49, 111, 445, 500, 634
MluCI AATT 4 cut(s) 289, 804, 864, 912
MlyI GAGTC 1 cut(s) 272
Mph1103I ATGCAT 1 cut(s) 670
MslI CAYNNNNRTG 6 cut(s) 159, 249, 389, 651, 732, 897
MspI CCGG 3 cut(s) 365, 767, 872
MspR9I CCNGG 4 cut(s) 681, 768, 843, 873
MvaI CCWGG 2 cut(s) 681, 843
MwoI GCNNNNNNNGC 3 cut(s) 536, 609, 665
NciI CCSGG 2 cut(s) 768, 873
NdeII GATC 3 cut(s) 616, 624, 643
NlaIII CATG 7 cut(s) 394, 422, 564, 650, 701, 856, 896
NlaIV GGNNCC 2 cut(s) 382, 517
NmuCI GTSAC 3 cut(s) 152, 161, 304
NsiI ATGCAT 1 cut(s) 670
NspI RCATGY 1 cut(s) 896
OliI CACNNNNGTG 1 cut(s) 159
PaeI GCATGC 1 cut(s) 896
PfoI TCCNGGA 2 cut(s) 841, 871
PleI GAGTC 1 cut(s) 271
PmaCI CACGTG 1 cut(s) 548
PmlI CACGTG 1 cut(s) 548
PpsI GAGTC 1 cut(s) 271
Ppu21I YACGTR 1 cut(s) 548
Psp6I CCWGG 2 cut(s) 679, 841
PspCI CACGTG 1 cut(s) 548
PspGI CCWGG 2 cut(s) 679, 841
PspN4I GGNNCC 2 cut(s) 382, 517
PspPI GGNCC 1 cut(s) 677
PstNI CAGNNNCTG 1 cut(s) 848
RsaI GTAC 1 cut(s) 382
RsaNI GTAC 1 cut(s) 381
RseI CAYNNNNRTG 6 cut(s) 159, 249, 389, 651, 732, 897
Sau3AI GATC 3 cut(s) 616, 624, 643
Sau96I GGNCC 1 cut(s) 677
SchI GAGTC 1 cut(s) 272
ScrFI CCNGG 4 cut(s) 681, 768, 843, 873
SfaNI GCATC 5 cut(s) 14, 186, 310, 336, 724
SmiMI CAYNNNNRTG 6 cut(s) 159, 249, 389, 651, 732, 897
SmlI CTYRAG 1 cut(s) 299
SmoI CTYRAG 1 cut(s) 299
SphI GCATGC 1 cut(s) 896
Sse9I AATT 4 cut(s) 289, 804, 864, 912
SsiI CCGC 1 cut(s) 896
StyD4I CCNGG 4 cut(s) 679, 766, 841, 871
StyI CCWWGG 1 cut(s) 457
TaaI ACNGT 4 cut(s) 331, 380, 436, 790
TaiI ACGT 3 cut(s) 550, 587, 749
TaqI TCGA 2 cut(s) 352, 777
TasI AATT 4 cut(s) 289, 804, 864, 912
TscAI CASTG 1 cut(s) 313
TseFI GTSAC 3 cut(s) 152, 161, 304
Tsp45I GTSAC 3 cut(s) 152, 161, 304
TspDTI ATGAA 1 cut(s) 49
TspRI CASTG 1 cut(s) 313
XapI RAATTY 1 cut(s) 864
XceI RCATGY 1 cut(s) 896
Zsp2I ATGCAT 1 cut(s) 670
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.