Prupe.3G278100_v2.0.a1

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
N/A
Physical Location & Seq
Reverse (-)
25521676 .. 25522329
654 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G278100.1_v2.0.a1.CDS.1

Sequence Viewer

Length: 654 bp
ATGGCAATACCAACAATGATGACTACTTTAGATTCGGCCATCAAAACTTACCAAATCAATTACCACCTCAAGGTCACCGGAACATTAGATGCGAAAACCGGTACCTCCATGAGATCAGGAAAGAAAAGGGGAGGACATGGTTCAACTCACACAGTTGCCACTTTTCTTTCTTCCAAGGAAGCCCAAAATGGCCTGCCAATAAGTATCACCTCACCTATGGTTTTCTTCAAGGCACCCCATCTGAGGCTGTGGGCGCAGTTGCACGTGATTTTGCAACATGGCAGGGCAACACACACTTCACGTTCAGCCAAGCCCAAAGCTTTGAGAGCGCTGAAGATCGGTTTTGGGAGGGGTGATCATGGAGATGGGGCTAATAATGCATTTGATGGCCCAGGTAAGACCGCTGCCCATGCATTTCGGCCAACAGATGGGAGATTTCACTACGATGCTGATGAGACGTGGCCGGTTGTGGGTGCTGTGCCTGGTGGTGTGGACTTGGAGACTGTTGCTTTGCATGAAATCGGGCACCTTCTTGGGCTTGGCCTTAGCTCTGTTCCTGGAGCTATCATGCTTCCTGAAATCCTCATTGGAGTGACGCGGCAGAGCTTGCATGCGGATGATATTCAAGGAATTAGAGCTTTATACAACACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

218

Amino Acids

23.17

Weight (kDa)

9.69

Isoelectric Point (pI)

29.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 101
AccB1I GGYRCC 3 cut(s) 101, 232, 525
AccB7I CCANNNNNTGG 1 cut(s) 428
AccII CGCG 1 cut(s) 598
AciI CCGC 3 cut(s) 402, 598, 614
AcoI YGGCCR 3 cut(s) 36, 419, 461
AcuI CTGAAG 1 cut(s) 353
AcvI CACGTG 1 cut(s) 265
AfaI GTAC 1 cut(s) 103
AfeI AGCGCT 1 cut(s) 330
AfiI CCNNNNNNNGG 4 cut(s) 70, 243, 428, 470
AgeI ACCGGT 1 cut(s) 98
AgsI TTSAA 3 cut(s) 144, 229, 626
AjiI CACGTC 1 cut(s) 459
AjnI CCWGG 3 cut(s) 391, 481, 556
AjuI GAANNNNNNNTTGG 2 cut(s) 570, 602
AluBI AGCT 5 cut(s) 320, 549, 563, 606, 638
AluI AGCT 5 cut(s) 320, 549, 563, 606, 638
Alw26I GTCTC 2 cut(s) 449, 494
Aor51HI AGCGCT 1 cut(s) 330
AoxI GGCC 6 cut(s) 36, 190, 388, 419, 461, 541
ApeKI GCWGC 1 cut(s) 404
ArsI GACNNNNNNTTYG 2 cut(s) 493, 525
AsiGI ACCGGT 1 cut(s) 98
Asp718I GGTACC 1 cut(s) 101
AspLEI GCGC 2 cut(s) 256, 331
AspS9I GGNCC 1 cut(s) 389
AsuHPI GGTGA 4 cut(s) 67, 199, 204, 365
BaeGI GKGCMC 1 cut(s) 528
BanI GGYRCC 3 cut(s) 101, 232, 525
BbrPI CACGTG 1 cut(s) 265
BbvI GCAGC 1 cut(s) 391
BccI CCATC 5 cut(s) 47, 246, 359, 380, 422
BciT130I CCWGG 3 cut(s) 393, 483, 558
BclI TGATCA 1 cut(s) 355
BcoDI GTCTC 2 cut(s) 449, 494
BfoI RGCGCY 1 cut(s) 332
BisI GCNGC 2 cut(s) 405, 599
BlsI GCNGC 2 cut(s) 406, 600
Bme1390I CCNGG 3 cut(s) 393, 483, 558
BmgBI CACGTC 1 cut(s) 459
BmgT120I GGNCC 1 cut(s) 389
BmiI GGNNCC 3 cut(s) 103, 234, 527
BmrFI CCNGG 3 cut(s) 393, 483, 558
BmsI GCATC 2 cut(s) 79, 436
BpmI CTGGAG 1 cut(s) 579
Bpu10I CCTNAGC 1 cut(s) 545
BpuEI CTTGAG 1 cut(s) 53
BsaAI YACGTR 1 cut(s) 265
BsaJI CCNNGG 2 cut(s) 174, 391
BsaWI WCCGGW 2 cut(s) 77, 98
Bsc4I CCNNNNNNNGG 4 cut(s) 70, 243, 428, 470
Bse118I RCCGGY 2 cut(s) 98, 463
BseBI CCWGG 3 cut(s) 393, 483, 558
BseDI CCNNGG 2 cut(s) 174, 391
BseGI GGATG 1 cut(s) 622
BseLI CCNNNNNNNGG 4 cut(s) 70, 243, 428, 470
BseMII CTCAG 1 cut(s) 233
BseSI GKGCMC 1 cut(s) 528
BseXI GCAGC 1 cut(s) 391
Bsh1236I CGCG 1 cut(s) 598
BshFI GGCC 6 cut(s) 38, 192, 390, 421, 463, 543
BshNI GGYRCC 3 cut(s) 101, 232, 525
BshTI ACCGGT 1 cut(s) 98
BsiSI CCGG 3 cut(s) 78, 99, 464
BslI CCNNNNNNNGG 4 cut(s) 70, 243, 428, 470
BsmAI GTCTC 2 cut(s) 449, 494
BsmBI CGTCTC 1 cut(s) 449
BsnI GGCC 6 cut(s) 38, 192, 390, 421, 463, 543
Bsp1286I GDGCHC 1 cut(s) 528
Bsp143I GATC 3 cut(s) 113, 336, 355
BspACI CCGC 3 cut(s) 402, 598, 614
BspANI GGCC 6 cut(s) 38, 192, 390, 421, 463, 543
BspCNI CTCAG 1 cut(s) 234
BspFNI CGCG 1 cut(s) 598
BspLI GGNNCC 3 cut(s) 103, 234, 527
BspT107I GGYRCC 3 cut(s) 101, 232, 525
BsrFI RCCGGY 2 cut(s) 98, 463
BssAI RCCGGY 2 cut(s) 98, 463
BssECI CCNNGG 2 cut(s) 174, 391
BssMI GATC 3 cut(s) 113, 336, 355
BssT1I CCWWGG 1 cut(s) 174
Bst2UI CCWGG 3 cut(s) 393, 483, 558
Bst4CI ACNGT 2 cut(s) 154, 505
BstAPI GCANNNNNTGC 1 cut(s) 607
BstBAI YACGTR 1 cut(s) 265
BstC8I GCNNGC 3 cut(s) 194, 608, 612
BstDEI CTNAG 2 cut(s) 242, 545
BstEII GGTNACC 1 cut(s) 73
BstF5I GGATG 1 cut(s) 622
BstFNI CGCG 1 cut(s) 598
BstH2I RGCGCY 1 cut(s) 332
BstHHI GCGC 2 cut(s) 256, 331
BstKTI GATC 3 cut(s) 116, 339, 358
BstMAI GTCTC 2 cut(s) 449, 494
BstMBI GATC 3 cut(s) 113, 336, 355
BstMWI GCNNNNNNNGC 5 cut(s) 253, 326, 377, 410, 607
BstNI CCWGG 3 cut(s) 393, 483, 558
BstNSI RCATGY 1 cut(s) 614
BstPI GGTNACC 1 cut(s) 73
BstSCI CCNGG 3 cut(s) 391, 481, 556
BstSLI GKGCMC 1 cut(s) 528
BstUI CGCG 1 cut(s) 598
BstV1I GCAGC 1 cut(s) 391
BsuRI GGCC 6 cut(s) 38, 192, 390, 421, 463, 543
BtrI CACGTC 1 cut(s) 459
BtsCI GGATG 1 cut(s) 622
Cac8I GCNNGC 3 cut(s) 194, 608, 612
CfoI GCGC 2 cut(s) 256, 331
Cfr10I RCCGGY 2 cut(s) 98, 463
Cfr13I GGNCC 1 cut(s) 389
CseI GACGC 1 cut(s) 604
Csp6I GTAC 1 cut(s) 102
CspAI ACCGGT 1 cut(s) 98
CviAII CATG 8 cut(s) 109, 137, 278, 359, 410, 515, 568, 611
CviQI GTAC 1 cut(s) 102
DdeI CTNAG 2 cut(s) 242, 545
DpnI GATC 3 cut(s) 115, 338, 357
DpnII GATC 3 cut(s) 113, 336, 355
EaeI YGGCCR 3 cut(s) 36, 419, 461
Eco130I CCWWGG 1 cut(s) 174
Eco47III AGCGCT 1 cut(s) 330
Eco57I CTGAAG 1 cut(s) 353
Eco72I CACGTG 1 cut(s) 265
Eco91I GGTNACC 1 cut(s) 73
EcoO65I GGTNACC 1 cut(s) 73
EcoRII CCWGG 3 cut(s) 391, 481, 556
EcoT14I CCWWGG 1 cut(s) 174
EcoT22I ATGCAT 2 cut(s) 382, 415
ErhI CCWWGG 1 cut(s) 174
Esp3I CGTCTC 1 cut(s) 449
FaeI CATG 8 cut(s) 112, 140, 281, 362, 413, 518, 571, 614
FatI CATG 8 cut(s) 108, 136, 277, 358, 409, 514, 567, 610
FbaI TGATCA 1 cut(s) 355
Fnu4HI GCNGC 2 cut(s) 405, 599
FokI GGATG 1 cut(s) 629
Fsp4HI GCNGC 2 cut(s) 405, 599
GlaI GCGC 2 cut(s) 255, 330
GluI GCNGC 2 cut(s) 405, 599
GsuI CTGGAG 1 cut(s) 579
HaeII RGCGCY 1 cut(s) 332
HaeIII GGCC 6 cut(s) 38, 192, 390, 421, 463, 543
HapII CCGG 3 cut(s) 78, 99, 464
HgaI GACGC 1 cut(s) 604
HhaI GCGC 2 cut(s) 256, 331
Hin1II CATG 8 cut(s) 112, 140, 281, 362, 413, 518, 571, 614
Hin6I GCGC 2 cut(s) 254, 329
HinP1I GCGC 2 cut(s) 254, 329
HindIII AAGCTT 1 cut(s) 318
HinfI GANTC 1 cut(s) 32
HpaII CCGG 3 cut(s) 78, 99, 464
HphI GGTGA 4 cut(s) 67, 199, 204, 365
Hpy166II GTNNAC 1 cut(s) 493
Hpy188I TCNGA 1 cut(s) 243
Hpy188III TCNNGA 2 cut(s) 117, 575
Hpy8I GTNNAC 1 cut(s) 493
HpyAV CCTTC 1 cut(s) 539
HpyCH4III ACNGT 2 cut(s) 154, 505
HpyCH4IV ACGT 3 cut(s) 264, 301, 458
HpyCH4V TGCA 6 cut(s) 262, 274, 380, 413, 514, 610
HpyF10VI GCNNNNNNNGC 5 cut(s) 253, 326, 377, 410, 607
HpyF3I CTNAG 2 cut(s) 242, 545
HpySE526I ACGT 3 cut(s) 264, 301, 458
Hsp92II CATG 8 cut(s) 112, 140, 281, 362, 413, 518, 571, 614
HspAI GCGC 2 cut(s) 254, 329
KpnI GGTACC 1 cut(s) 105
Ksp22I TGATCA 1 cut(s) 355
Kzo9I GATC 3 cut(s) 113, 336, 355
LmnI GCTCC 1 cut(s) 560
Lsp1109I GCAGC 1 cut(s) 391
LweI GCATC 2 cut(s) 79, 436
MaeII ACGT 3 cut(s) 264, 301, 458
MaeIII GTNAC 2 cut(s) 73, 592
MalI GATC 3 cut(s) 115, 338, 357
MboI GATC 3 cut(s) 113, 336, 355
MboII GAAGA 3 cut(s) 162, 217, 346
MhlI GDGCHC 1 cut(s) 528
MluCI AATT 2 cut(s) 58, 630
MnlI CCTC 7 cut(s) 77, 115, 125, 220, 237, 342, 593
Mph1103I ATGCAT 2 cut(s) 382, 415
MslI CAYNNNNRTG 4 cut(s) 363, 444, 590, 615
MspA1I CMGCKG 1 cut(s) 404
MspI CCGG 3 cut(s) 78, 99, 464
MspR9I CCNGG 3 cut(s) 393, 483, 558
MvaI CCWGG 3 cut(s) 393, 483, 558
MvnI CGCG 1 cut(s) 598
MwoI GCNNNNNNNGC 5 cut(s) 253, 326, 377, 410, 607
NdeII GATC 3 cut(s) 113, 336, 355
NlaIII CATG 8 cut(s) 112, 140, 281, 362, 413, 518, 571, 614
NlaIV GGNNCC 3 cut(s) 103, 234, 527
NmuCI GTSAC 2 cut(s) 73, 592
NsiI ATGCAT 2 cut(s) 382, 415
NspI RCATGY 1 cut(s) 614
PaeI GCATGC 1 cut(s) 614
PfeI GAWTC 1 cut(s) 32
PflMI CCANNNNNTGG 1 cut(s) 428
PfoI TCCNGGA 1 cut(s) 556
PinAI ACCGGT 1 cut(s) 98
PkrI GCNGC 2 cut(s) 406, 600
PmaCI CACGTG 1 cut(s) 265
PmlI CACGTG 1 cut(s) 265
Ppu21I YACGTR 1 cut(s) 265
Psp6I CCWGG 3 cut(s) 391, 481, 556
PspCI CACGTG 1 cut(s) 265
PspEI GGTNACC 1 cut(s) 73
PspGI CCWGG 3 cut(s) 391, 481, 556
PspN4I GGNNCC 3 cut(s) 103, 234, 527
PspPI GGNCC 1 cut(s) 389
RsaI GTAC 1 cut(s) 103
RsaNI GTAC 1 cut(s) 102
RseI CAYNNNNRTG 4 cut(s) 363, 444, 590, 615
SatI GCNGC 2 cut(s) 405, 599
Sau3AI GATC 3 cut(s) 113, 336, 355
Sau96I GGNCC 1 cut(s) 389
ScrFI CCNGG 3 cut(s) 393, 483, 558
SduI GDGCHC 1 cut(s) 528
SfaNI GCATC 2 cut(s) 79, 436
SmiMI CAYNNNNRTG 4 cut(s) 363, 444, 590, 615
SmlI CTYRAG 1 cut(s) 68
SmoI CTYRAG 1 cut(s) 68
SphI GCATGC 1 cut(s) 614
Sse9I AATT 2 cut(s) 58, 630
SsiI CCGC 3 cut(s) 402, 598, 614
StyD4I CCNGG 3 cut(s) 391, 481, 556
StyI CCWWGG 1 cut(s) 174
TaaI ACNGT 2 cut(s) 154, 505
TaiI ACGT 3 cut(s) 267, 304, 461
TasI AATT 2 cut(s) 58, 630
TauI GCSGC 1 cut(s) 601
TfiI GAWTC 1 cut(s) 32
TseFI GTSAC 2 cut(s) 73, 592
TseI GCWGC 1 cut(s) 404
Tsp45I GTSAC 2 cut(s) 73, 592
TspDTI ATGAA 1 cut(s) 531
Van91I CCANNNNNTGG 1 cut(s) 428
XceI RCATGY 1 cut(s) 614
Zsp2I ATGCAT 2 cut(s) 382, 415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.