Prupe.3G284500_v2.0.a1

2-methylene-furan-3-one reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
N/A
Physical Location & Seq
Forward (+)
25789448 .. 25791723
2276 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G284500.1_v2.0.a1.CDS.6

Sequence Viewer

Length: 729 bp
ATGCCTCTTCCTCAAGTAGAGGAAGACCAGGTGCTCATCAAGGTGGCTGCTGCAGCACTTAACCCCATTGATCACACGAGGCTTCTTGGGTATTTCAAGGACACTGACAGTGCTCTACCTACAGTTCCAGGGTATGATGTTGCTGGTGTAGTGGTGAAGCTTGGAAGCCAAGCAAGGAAATTTAAGGCGGGGATGAAGTATATAGGACTATCAATGAGCAAGAAAATTGGGTCGTTAGCAGAGTACACGGCTATGGAAGAAACATTGTTGGCTGTGAAACCCAAAAATCTGAGTTTTGCTGAGGCTGCTAGCCCTCCTGTGGCTATTGAGACTGCCTATGAAGGGCTCGAACGAGCTCAAATTTTTGCTGGTAAATCCATTCTTGTTTTAGGAGGTGCTGGGGATGTAGGAACCCATGTTATTCAGCATGTTTTTGGTGTTTCCAGAGTGGCAGCTACTGCAAGCACTAAAAAACTGGATTCGTTGAGGAGCTTGGGTGCTGATCTTGCTATTGATTATACGAAGGACAACTTCGAAGAGCTGCCTGAGAAATTTGATGTGGTCTATGATGCAGTTGAAACTCAGCCCTTACTTGGAGAGTGTGGGAAGGTGAAGGCAGTGATTGATCCGAATGGCCCATATCCCTTTCCTAATACCCTTGAAGCATTTGCCTATCTTGAAGCTAGTAGCAGAGCTATAGGAAAGCGAGCATGTTATGTTCCCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N ADH_zinc_N ADH_zinc_N_2

Protein Analysis

243

Amino Acids

25.86

Weight (kDa)

5.71

Isoelectric Point (pI)

35.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 188
AclWI GGATC 1 cut(s) 620
AcsI RAATTY 3 cut(s) 179, 360, 551
AfaI GTAC 1 cut(s) 245
AfiI CCNNNNNNNGG 3 cut(s) 319, 342, 593
AgsI TTSAA 4 cut(s) 97, 578, 662, 680
AjnI CCWGG 2 cut(s) 27, 127
AluBI AGCT 7 cut(s) 160, 356, 455, 492, 541, 683, 695
AluI AGCT 7 cut(s) 160, 356, 455, 492, 541, 683, 695
Alw21I GWGCWC 3 cut(s) 36, 115, 358
Alw26I GTCTC 1 cut(s) 323
AlwI GGATC 1 cut(s) 620
AlwNI CAGNNNCTG 1 cut(s) 458
AoxI GGCC 1 cut(s) 634
ApeKI GCWGC 6 cut(s) 47, 50, 53, 305, 452, 541
ApoI RAATTY 3 cut(s) 179, 360, 551
AspS9I GGNCC 1 cut(s) 635
AsuHPI GGTGA 2 cut(s) 166, 622
AsuII TTCGAA 1 cut(s) 534
AsuNHI GCTAGC 1 cut(s) 308
BanII GRGCYC 2 cut(s) 348, 358
BauI CACGAG 1 cut(s) 76
BbsI GAAGAC 1 cut(s) 30
Bbv12I GWGCWC 3 cut(s) 36, 115, 358
BbvCI CCTCAGC 1 cut(s) 300
BbvI GCAGC 6 cut(s) 34, 37, 65, 292, 464, 528
BceAI ACGGC 1 cut(s) 264
BciT130I CCWGG 2 cut(s) 29, 129
BclI TGATCA 1 cut(s) 70
BcoDI GTCTC 1 cut(s) 323
BfaI CTAG 2 cut(s) 309, 684
BfmI CTRYAG 3 cut(s) 51, 120, 696
BisI GCNGC 6 cut(s) 48, 51, 54, 306, 453, 542
BlsI GCNGC 6 cut(s) 49, 52, 55, 307, 454, 543
Bme1390I CCNGG 2 cut(s) 29, 129
BmgT120I GGNCC 1 cut(s) 635
BmiI GGNNCC 1 cut(s) 412
BmrFI CCNGG 2 cut(s) 29, 129
BmsI GCATC 1 cut(s) 559
BmtI GCTAGC 1 cut(s) 312
BpiI GAAGAC 1 cut(s) 30
Bpu10I CCTNAGC 1 cut(s) 300
Bpu14I TTCGAA 1 cut(s) 534
BsaJI CCNNGG 1 cut(s) 128
BsaXI ACNNNNNCTCC 2 cut(s) 481, 511
Bsc4I CCNNNNNNNGG 3 cut(s) 319, 342, 593
Bse1I ACTGG 1 cut(s) 480
BseBI CCWGG 2 cut(s) 29, 129
BseDI CCNNGG 1 cut(s) 128
BseGI GGATG 2 cut(s) 198, 409
BseLI CCNNNNNNNGG 3 cut(s) 319, 342, 593
BseMII CTCAG 4 cut(s) 281, 291, 537, 596
BseNI ACTGG 1 cut(s) 480
BseRI GAGGAG 1 cut(s) 502
BseXI GCAGC 6 cut(s) 34, 37, 65, 292, 464, 528
BseYI CCCAGC 1 cut(s) 398
BshFI GGCC 1 cut(s) 636
BsiHKAI GWGCWC 3 cut(s) 36, 115, 358
BslI CCNNNNNNNGG 3 cut(s) 319, 342, 593
BsmAI GTCTC 1 cut(s) 323
BsnI GGCC 1 cut(s) 636
Bsp119I TTCGAA 1 cut(s) 534
Bsp1286I GDGCHC 4 cut(s) 36, 115, 348, 358
Bsp143I GATC 3 cut(s) 70, 502, 625
BspACI CCGC 1 cut(s) 188
BspANI GGCC 1 cut(s) 636
BspCNI CTCAG 4 cut(s) 282, 292, 538, 595
BspLI GGNNCC 1 cut(s) 412
BspMAI CTGCAG 1 cut(s) 55
BspOI GCTAGC 1 cut(s) 312
BspPI GGATC 1 cut(s) 620
BspQI GCTCTTC 1 cut(s) 531
BspT104I TTCGAA 1 cut(s) 534
BsrI ACTGG 1 cut(s) 480
BssECI CCNNGG 1 cut(s) 128
BssMI GATC 3 cut(s) 70, 502, 625
BssSI CACGAG 1 cut(s) 76
Bst2BI CACGAG 1 cut(s) 76
Bst2UI CCWGG 2 cut(s) 29, 129
Bst4CI ACNGT 2 cut(s) 110, 124
Bst6I CTCTTC 2 cut(s) 12, 531
BstAPI GCANNNNNTGC 1 cut(s) 458
BstBI TTCGAA 1 cut(s) 534
BstC8I GCNNGC 3 cut(s) 310, 463, 708
BstDEI CTNAG 4 cut(s) 290, 300, 546, 582
BstF5I GGATG 2 cut(s) 198, 409
BstKTI GATC 3 cut(s) 73, 505, 628
BstMAI GTCTC 1 cut(s) 323
BstMBI GATC 3 cut(s) 70, 502, 625
BstMWI GCNNNNNNNGC 4 cut(s) 53, 305, 458, 506
BstNI CCWGG 2 cut(s) 29, 129
BstNSI RCATGY 2 cut(s) 431, 714
BstSCI CCNGG 2 cut(s) 27, 127
BstSFI CTRYAG 3 cut(s) 51, 120, 696
BstV1I GCAGC 6 cut(s) 34, 37, 65, 292, 464, 528
BstV2I GAAGAC 1 cut(s) 30
BsuRI GGCC 1 cut(s) 636
BtsCI GGATG 2 cut(s) 198, 409
BtsI GCAGTG 1 cut(s) 624
BtsIMutI CAGTG 3 cut(s) 102, 115, 624
Cac8I GCNNGC 3 cut(s) 310, 463, 708
CaiI CAGNNNCTG 1 cut(s) 458
Cfr13I GGNCC 1 cut(s) 635
CsiI ACCWGGT 1 cut(s) 27
Csp6I GTAC 1 cut(s) 244
CviAII CATG 3 cut(s) 416, 428, 711
CviQI GTAC 1 cut(s) 244
DdeI CTNAG 4 cut(s) 290, 300, 546, 582
DpnI GATC 3 cut(s) 72, 504, 627
DpnII GATC 3 cut(s) 70, 502, 625
Eam1104I CTCTTC 2 cut(s) 12, 531
EarI CTCTTC 2 cut(s) 12, 531
Ecl136II GAGCTC 1 cut(s) 356
Eco24I GRGCYC 2 cut(s) 348, 358
Eco53kI GAGCTC 1 cut(s) 356
EcoICRI GAGCTC 1 cut(s) 356
EcoRII CCWGG 2 cut(s) 27, 127
EcoT38I GRGCYC 2 cut(s) 348, 358
FaeI CATG 3 cut(s) 419, 431, 714
FalI AAGNNNNNCTT 2 cut(s) 515, 547
FatI CATG 3 cut(s) 415, 427, 710
FauI CCCGC 1 cut(s) 181
FbaI TGATCA 1 cut(s) 70
Fnu4HI GCNGC 6 cut(s) 48, 51, 54, 306, 453, 542
FokI GGATG 2 cut(s) 205, 416
FriOI GRGCYC 2 cut(s) 348, 358
Fsp4HI GCNGC 6 cut(s) 48, 51, 54, 306, 453, 542
FspBI CTAG 2 cut(s) 309, 684
GluI GCNGC 6 cut(s) 48, 51, 54, 306, 453, 542
GsaI CCCAGC 1 cut(s) 402
HaeIII GGCC 1 cut(s) 636
Hin1II CATG 3 cut(s) 419, 431, 714
HindIII AAGCTT 1 cut(s) 158
HinfI GANTC 1 cut(s) 479
HphI GGTGA 2 cut(s) 166, 622
Hpy166II GTNNAC 1 cut(s) 246
Hpy188I TCNGA 2 cut(s) 291, 630
Hpy188III TCNNGA 2 cut(s) 444, 677
Hpy8I GTNNAC 1 cut(s) 246
HpyAV CCTTC 4 cut(s) 335, 517, 601, 607
HpyCH4III ACNGT 2 cut(s) 110, 124
HpyCH4V TGCA 3 cut(s) 53, 461, 572
HpyF10VI GCNNNNNNNGC 4 cut(s) 53, 305, 458, 506
HpyF3I CTNAG 4 cut(s) 290, 300, 546, 582
Hsp92II CATG 3 cut(s) 419, 431, 714
Ksp22I TGATCA 1 cut(s) 70
Kzo9I GATC 3 cut(s) 70, 502, 625
LguI GCTCTTC 1 cut(s) 531
LmnI GCTCC 1 cut(s) 489
Lsp1109I GCAGC 6 cut(s) 34, 37, 65, 292, 464, 528
LweI GCATC 1 cut(s) 559
MabI ACCWGGT 1 cut(s) 27
MaeI CTAG 2 cut(s) 309, 684
MalI GATC 3 cut(s) 72, 504, 627
MboI GATC 3 cut(s) 70, 502, 625
MboII GAAGA 3 cut(s) 35, 269, 548
MhlI GDGCHC 4 cut(s) 36, 115, 348, 358
MluCI AATT 4 cut(s) 179, 225, 360, 551
MnlI CCTC 8 cut(s) 13, 15, 21, 72, 295, 324, 386, 480
MseI TTAA 2 cut(s) 60, 183
MslI CAYNNNNRTG 2 cut(s) 41, 251
MspR9I CCNGG 2 cut(s) 29, 129
MvaI CCWGG 2 cut(s) 29, 129
MwoI GCNNNNNNNGC 4 cut(s) 53, 305, 458, 506
NdeII GATC 3 cut(s) 70, 502, 625
NheI GCTAGC 1 cut(s) 308
NlaIII CATG 3 cut(s) 419, 431, 714
NlaIV GGNNCC 1 cut(s) 412
NspI RCATGY 2 cut(s) 431, 714
NspV TTCGAA 1 cut(s) 534
PciSI GCTCTTC 1 cut(s) 531
PfeI GAWTC 1 cut(s) 479
PkrI GCNGC 6 cut(s) 49, 52, 55, 307, 454, 543
Psp124BI GAGCTC 1 cut(s) 358
Psp6I CCWGG 2 cut(s) 27, 127
PspFI CCCAGC 1 cut(s) 398
PspGI CCWGG 2 cut(s) 27, 127
PspN4I GGNNCC 1 cut(s) 412
PspPI GGNCC 1 cut(s) 635
PstI CTGCAG 1 cut(s) 55
PstNI CAGNNNCTG 1 cut(s) 458
RsaI GTAC 1 cut(s) 245
RsaNI GTAC 1 cut(s) 244
RseI CAYNNNNRTG 2 cut(s) 41, 251
SacI GAGCTC 1 cut(s) 358
SapI GCTCTTC 1 cut(s) 531
SaqAI TTAA 2 cut(s) 60, 183
SatI GCNGC 6 cut(s) 48, 51, 54, 306, 453, 542
Sau3AI GATC 3 cut(s) 70, 502, 625
Sau96I GGNCC 1 cut(s) 635
ScrFI CCNGG 2 cut(s) 29, 129
SduI GDGCHC 4 cut(s) 36, 115, 348, 358
SexAI ACCWGGT 1 cut(s) 27
SfaNI GCATC 1 cut(s) 559
SfcI CTRYAG 3 cut(s) 51, 120, 696
SfuI TTCGAA 1 cut(s) 534
SmiMI CAYNNNNRTG 2 cut(s) 41, 251
SmlI CTYRAG 1 cut(s) 12
SmoI CTYRAG 1 cut(s) 12
Sse9I AATT 4 cut(s) 179, 225, 360, 551
SsiI CCGC 1 cut(s) 188
SspMI CTAG 2 cut(s) 309, 684
SstI GAGCTC 1 cut(s) 358
StyD4I CCNGG 2 cut(s) 27, 127
TaaI ACNGT 2 cut(s) 110, 124
TaqI TCGA 2 cut(s) 348, 534
TasI AATT 4 cut(s) 179, 225, 360, 551
TatI WGTACW 1 cut(s) 243
TfiI GAWTC 1 cut(s) 479
Tru1I TTAA 2 cut(s) 60, 183
Tru9I TTAA 2 cut(s) 60, 183
TscAI CASTG 3 cut(s) 109, 115, 624
TseI GCWGC 6 cut(s) 47, 50, 53, 305, 452, 541
TspDTI ATGAA 2 cut(s) 209, 354
TspRI CASTG 3 cut(s) 109, 115, 624
XapI RAATTY 3 cut(s) 179, 360, 551
XceI RCATGY 2 cut(s) 431, 714
XspI CTAG 2 cut(s) 309, 684
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.