Prupe.4G021800_v2.0.a1

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
N/A
Physical Location & Seq
Forward (+)
1032317 .. 1035005
2689 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G021800.1_v2.0.a1.CDS.5

Sequence Viewer

Length: 1518 bp
ATGGAGAACATGGACAGCGATCAGCACAATGAAGCCTTGTTACAATCCGGAGAAGCATCAGCGAGAGGGCCTATAAGTTCAGCACTGGAAGATATACTAACCAACCCTGATCTCTCATACTTCCAGCGCCTTGGGCAGGCTTCTTGGCGTGAAATTAAGACCCTCTTCTACCTAGCAGCTCCGGCGGTGGGGAATTACTTGCTCAGCAACATCACTGCAGTAGCCACCCTCATCTTCTGTGGCCGACTTGGCAATCTTGAATTCGCTGGTGCCTCTCTAGGGAACACTGGTGTCGCTGTCTTTGCTTACGGCCTCATGCTTGGAATGGGAAGTGCAGTGGAGACATTGTGTGGCCAAGCATTTGGAGCAAATAAGAACGAAATGCTGGGTGTATATTTACAGCGAGCAACAATTCTTCTCACGGCAACAGCAATCCCAGTGATGTTCATCTACATCTTCTCCAAGCAAATCTTGATCGGACTAGGCGAAACGGAGAGCATAGCATCTGCAGGAGCACTCTTCGTCTATGGCCTAATTCCACAGCTCTTTGCCCTGGCTTTCAATTTTCCCTTACAAAAATTCTTACAAGCTCAGAGCATAGTGTGGCCTAGCGCCTGCATATCTGGCGTTACAGTTGTGGTGCACTTAGTACTTAGTTGGGTGGTGGTGTACAAATTGGGGTGGGGTTTGCTGGGTGCATCTTTGGTTTTGAGCTTGTCCTGGTGGATCATGGTGGTGGCCCAGTTAATGTACATCTTGGTGAGCCCAAGGTGCAAATATACTTGGACTGGATTTAGCTCACAGGCTTTTACTGGGCTCTGGGAGTTTCTGAAGTTATCCACTGCATCCGCAGTCATGCTGTGCCTTGAGACTTGGTACTTTCAGATTTTGGTTTTGATCGCTGGGTTGCTCAAGAATGCTGAGATTGCCTTGGACGCACTCTCTGTTTGCATGGCTGTATCTGGATGGGTGTTCATGATCTCTGTTGGGTTCAATGCTGCAGCAAGTATAAGGGTGAGCAATGAGGTTGGTGCTGGGCATCCAAAATCAGCATCATTTGCAGTGGTGGTTGTAACTACAAGCTCCTTAATCATCTCCTTAATCCTTGCCATAATCATACTTGCACTCCGCCGTTACCTCAGCTATATATTCACTGAGGGCTCCACTGTTTCTAATGCCGTCGCTGAACTTTCTCCCTTCTTGGCTTGTACTCTTTTACTTAACGGCATCCAACCTGTTTTGTCTGGGGTGGCTGTTGGGTGTGGATGGCAAGCAATCGTGGCATGGATTAACGTAGGATGTTACTACATCATTGGTATCCCACTGGGTTGTGTTCTTGGCTTCAAGCTTGACTTAGGAGCTAAGGGAATATGGTCGGGAATGCTGGCGGGCACTTTTATTCAAACTCTTATACTCTGTTGGATCACATTCCGGACTGACTGGAATAAAGAGATTGCGAAATCAAAGATTCGTATAGATGAATGGGAAGACAAGAAGGAGCCTCTTCTCAATGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

506

Amino Acids

54.58

Weight (kDa)

5.77

Isoelectric Point (pI)

28.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 269
AccIII TCCGGA 2 cut(s) 47, 1431
AciI CCGC 4 cut(s) 185, 849, 1129, 1388
AclWI GGATC 2 cut(s) 734, 1430
AcoI YGGCCR 2 cut(s) 241, 352
AcsI RAATTY 2 cut(s) 260, 578
AcuI CTGAAG 1 cut(s) 851
AfaI GTAC 5 cut(s) 651, 671, 752, 878, 1210
AfiI CCNNNNNNNGG 3 cut(s) 136, 188, 279
AgsI TTSAA 5 cut(s) 260, 562, 994, 1345, 1403
AjnI CCWGG 2 cut(s) 552, 719
AluBI AGCT 9 cut(s) 179, 544, 590, 714, 798, 1083, 1143, 1348, 1361
AluI AGCT 9 cut(s) 179, 544, 590, 714, 798, 1083, 1143, 1348, 1361
Alw21I GWGCWC 2 cut(s) 517, 645
Alw26I GTCTC 2 cut(s) 335, 863
Alw44I GTGCAC 1 cut(s) 641
AlwI GGATC 2 cut(s) 734, 1430
Aor13HI TCCGGA 2 cut(s) 47, 1431
AoxI GGCC 7 cut(s) 68, 241, 310, 352, 529, 605, 738
ApaLI GTGCAC 1 cut(s) 641
ApeKI GCWGC 3 cut(s) 176, 998, 1001
ApoI RAATTY 2 cut(s) 260, 578
AspLEI GCGC 2 cut(s) 129, 614
AspS9I GGNCC 2 cut(s) 68, 739
AsuHPI GGTGA 2 cut(s) 772, 1027
BaeGI GKGCMC 2 cut(s) 645, 1394
BalI TGGCCA 1 cut(s) 354
BanI GGYRCC 1 cut(s) 269
BanII GRGCYC 3 cut(s) 767, 819, 1163
BarI GAAGNNNNNNTAC 2 cut(s) 24, 56
BbsI GAAGAC 1 cut(s) 1494
Bbv12I GWGCWC 2 cut(s) 517, 645
BbvCI CCTCAGC 1 cut(s) 1139
BbvI GCAGC 3 cut(s) 188, 985, 1013
BccI CCATC 2 cut(s) 960, 1260
BceAI ACGGC 5 cut(s) 325, 438, 1116, 1163, 1240
BciT130I CCWGG 2 cut(s) 554, 721
BciVI GTATCC 1 cut(s) 1328
BcoDI GTCTC 2 cut(s) 335, 863
BfaI CTAG 4 cut(s) 173, 278, 482, 609
BfmI CTRYAG 3 cut(s) 216, 507, 999
BfoI RGCGCY 2 cut(s) 130, 615
BfuI GTATCC 1 cut(s) 1328
BglI GCCNNNNNGGC 1 cut(s) 249
BisI GCNGC 3 cut(s) 177, 999, 1002
BlpI GCTNAGC 1 cut(s) 203
BlsI GCNGC 3 cut(s) 178, 1000, 1003
BmcAI AGTACT 1 cut(s) 651
Bme1390I CCNGG 2 cut(s) 554, 721
BmgT120I GGNCC 2 cut(s) 68, 739
BmiI GGNNCC 3 cut(s) 271, 1162, 1500
BmrFI CCNGG 2 cut(s) 554, 721
BmrI ACTGGG 4 cut(s) 431, 736, 822, 1334
BmsI GCATC 7 cut(s) 65, 512, 707, 854, 1048, 1061, 1236
BmuI ACTGGG 4 cut(s) 431, 736, 822, 1334
BpiI GAAGAC 1 cut(s) 1494
Bpu10I CCTNAGC 2 cut(s) 1139, 1362
Bpu1102I GCTNAGC 1 cut(s) 203
BpuEI CTTGAG 2 cut(s) 887, 896
BsaBI GATNNNNATC 1 cut(s) 446
BsaJI CCNNGG 4 cut(s) 130, 552, 767, 930
BsaWI WCCGGW 2 cut(s) 47, 1431
BsaXI ACNNNNNCTCC 6 cut(s) 332, 362, 443, 473, 1110, 1140
Bsc4I CCNNNNNNNGG 3 cut(s) 136, 188, 279
Bse1I ACTGG 8 cut(s) 90, 292, 437, 742, 793, 817, 1329, 1445
Bse3DI GCAATG 1 cut(s) 1027
Bse8I GATNNNNATC 1 cut(s) 446
BseAI TCCGGA 2 cut(s) 47, 1431
BseBI CCWGG 2 cut(s) 554, 721
BseDI CCNNGG 4 cut(s) 130, 552, 767, 930
BseGI GGATG 6 cut(s) 845, 971, 1039, 1227, 1271, 1304
BseJI GATNNNNATC 1 cut(s) 446
BseLI CCNNNNNNNGG 3 cut(s) 136, 188, 279
BseMI GCAATG 1 cut(s) 1027
BseMII CTCAG 5 cut(s) 217, 605, 912, 1146, 1153
BseNI ACTGG 8 cut(s) 90, 292, 437, 742, 793, 817, 1329, 1445
BseSI GKGCMC 2 cut(s) 645, 1394
BseXI GCAGC 3 cut(s) 188, 985, 1013
BseYI CCCAGC 4 cut(s) 385, 691, 902, 1034
BsgI GTGCAG 1 cut(s) 354
BshFI GGCC 7 cut(s) 70, 243, 312, 354, 531, 607, 740
BshNI GGYRCC 1 cut(s) 269
BsiHKAI GWGCWC 2 cut(s) 517, 645
BsiSI CCGG 3 cut(s) 48, 182, 1432
BslI CCNNNNNNNGG 3 cut(s) 136, 188, 279
BsmAI GTCTC 2 cut(s) 335, 863
BsmI GAATGC 2 cut(s) 922, 1386
BsnI GGCC 7 cut(s) 70, 243, 312, 354, 531, 607, 740
Bsp1286I GDGCHC 6 cut(s) 517, 645, 767, 819, 1163, 1394
Bsp13I TCCGGA 2 cut(s) 47, 1431
Bsp1407I TGTACA 2 cut(s) 669, 750
Bsp143I GATC 7 cut(s) 19, 109, 474, 726, 897, 978, 1422
Bsp1720I GCTNAGC 1 cut(s) 203
BspACI CCGC 4 cut(s) 185, 849, 1129, 1388
BspANI GGCC 7 cut(s) 70, 243, 312, 354, 531, 607, 740
BspCNI CTCAG 5 cut(s) 216, 604, 913, 1147, 1152
BspEI TCCGGA 2 cut(s) 47, 1431
BspHI TCATGA 1 cut(s) 975
BspLI GGNNCC 3 cut(s) 271, 1162, 1500
BspMAI CTGCAG 3 cut(s) 220, 511, 1003
BspPI GGATC 2 cut(s) 734, 1430
BspT107I GGYRCC 1 cut(s) 269
BsrDI GCAATG 1 cut(s) 1027
BsrGI TGTACA 2 cut(s) 669, 750
BsrI ACTGG 8 cut(s) 90, 292, 437, 742, 793, 817, 1329, 1445
BssECI CCNNGG 4 cut(s) 130, 552, 767, 930
BssMI GATC 7 cut(s) 19, 109, 474, 726, 897, 978, 1422
BssT1I CCWWGG 3 cut(s) 130, 767, 930
Bst2UI CCWGG 2 cut(s) 554, 721
Bst4CI ACNGT 2 cut(s) 634, 1168
Bst6I CTCTTC 3 cut(s) 170, 524, 1509
BstAPI GCANNNNNTGC 1 cut(s) 1058
BstAUI TGTACA 2 cut(s) 669, 750
BstC8I GCNNGC 6 cut(s) 138, 405, 616, 1272, 1386, 1390
BstDEI CTNAG 9 cut(s) 203, 591, 646, 653, 921, 1139, 1155, 1354, 1362
BstENI CCTNNNNNAGG 1 cut(s) 134
BstF5I GGATG 6 cut(s) 845, 971, 1039, 1227, 1271, 1304
BstH2I RGCGCY 2 cut(s) 130, 615
BstHHI GCGC 2 cut(s) 129, 614
BstKTI GATC 7 cut(s) 22, 112, 477, 729, 900, 981, 1425
BstMAI GTCTC 2 cut(s) 335, 863
BstMBI GATC 7 cut(s) 19, 109, 474, 726, 897, 978, 1422
BstNI CCWGG 2 cut(s) 554, 721
BstSCI CCNGG 2 cut(s) 552, 719
BstSFI CTRYAG 3 cut(s) 216, 507, 999
BstSLI GKGCMC 2 cut(s) 645, 1394
BstV1I GCAGC 3 cut(s) 188, 985, 1013
BstV2I GAAGAC 1 cut(s) 1494
BstXI CCANNNNNNTGG 2 cut(s) 131, 362
BsuI GTATCC 1 cut(s) 1328
BsuRI GGCC 7 cut(s) 70, 243, 312, 354, 531, 607, 740
BtsCI GGATG 6 cut(s) 845, 971, 1039, 1227, 1271, 1304
BtsI GCAGTG 4 cut(s) 213, 342, 840, 1068
Cac8I GCNNGC 6 cut(s) 138, 405, 616, 1272, 1386, 1390
CciI TCATGA 1 cut(s) 975
CfoI GCGC 2 cut(s) 129, 614
Cfr13I GGNCC 2 cut(s) 68, 739
CseI GACGC 1 cut(s) 944
Csp6I GTAC 5 cut(s) 650, 670, 751, 877, 1209
CviAII CATG 7 cut(s) 10, 316, 730, 856, 952, 976, 1284
CviQI GTAC 5 cut(s) 650, 670, 751, 877, 1209
DdeI CTNAG 9 cut(s) 203, 591, 646, 653, 921, 1139, 1155, 1354, 1362
DpnI GATC 7 cut(s) 21, 111, 476, 728, 899, 980, 1424
DpnII GATC 7 cut(s) 19, 109, 474, 726, 897, 978, 1422
EaeI YGGCCR 2 cut(s) 241, 352
Eam1104I CTCTTC 3 cut(s) 170, 524, 1509
EarI CTCTTC 3 cut(s) 170, 524, 1509
EciI GGCGGA 1 cut(s) 1118
Eco130I CCWWGG 3 cut(s) 130, 767, 930
Eco24I GRGCYC 3 cut(s) 767, 819, 1163
Eco57I CTGAAG 1 cut(s) 851
EcoNI CCTNNNNNAGG 1 cut(s) 134
EcoO109I RGGNCCY 1 cut(s) 68
EcoRI GAATTC 1 cut(s) 260
EcoRII CCWGG 2 cut(s) 552, 719
EcoT14I CCWWGG 3 cut(s) 130, 767, 930
EcoT38I GRGCYC 3 cut(s) 767, 819, 1163
ErhI CCWWGG 3 cut(s) 130, 767, 930
FaeI CATG 7 cut(s) 13, 319, 733, 859, 955, 979, 1287
FalI AAGNNNNNCTT 6 cut(s) 149, 181, 455, 487, 1337, 1369
FatI CATG 7 cut(s) 9, 315, 729, 855, 951, 975, 1283
FauI CCCGC 1 cut(s) 1381
Fnu4HI GCNGC 3 cut(s) 177, 999, 1002
FokI GGATG 6 cut(s) 832, 978, 1026, 1214, 1278, 1311
FriOI GRGCYC 3 cut(s) 767, 819, 1163
Fsp4HI GCNGC 3 cut(s) 177, 999, 1002
FspBI CTAG 4 cut(s) 173, 278, 482, 609
GlaI GCGC 2 cut(s) 128, 613
GluI GCNGC 3 cut(s) 177, 999, 1002
GsaI CCCAGC 4 cut(s) 389, 695, 906, 1038
HaeII RGCGCY 2 cut(s) 130, 615
HaeIII GGCC 7 cut(s) 70, 243, 312, 354, 531, 607, 740
HapII CCGG 3 cut(s) 48, 182, 1432
HgaI GACGC 1 cut(s) 944
HhaI GCGC 2 cut(s) 129, 614
Hin1II CATG 7 cut(s) 13, 319, 733, 859, 955, 979, 1287
Hin6I GCGC 2 cut(s) 127, 612
HinP1I GCGC 2 cut(s) 127, 612
HindIII AAGCTT 1 cut(s) 1346
HinfI GANTC 1 cut(s) 1468
HpaII CCGG 3 cut(s) 48, 182, 1432
HphI GGTGA 2 cut(s) 772, 1027
Hpy166II GTNNAC 2 cut(s) 643, 670
Hpy188I TCNGA 4 cut(s) 479, 594, 831, 885
Hpy188III TCNNGA 8 cut(s) 48, 257, 472, 913, 963, 976, 1377, 1432
Hpy8I GTNNAC 2 cut(s) 643, 670
Hpy99I CGWCG 1 cut(s) 1184
HpyAV CCTTC 2 cut(s) 1207, 1489
HpyCH4III ACNGT 2 cut(s) 634, 1168
HpyCH4IV ACGT 1 cut(s) 1293
HpyF3I CTNAG 9 cut(s) 203, 591, 646, 653, 921, 1139, 1155, 1354, 1362
HpySE526I ACGT 1 cut(s) 1293
Hsp92II CATG 7 cut(s) 13, 319, 733, 859, 955, 979, 1287
HspAI GCGC 2 cut(s) 127, 612
Kpn2I TCCGGA 2 cut(s) 47, 1431
Kzo9I GATC 7 cut(s) 19, 109, 474, 726, 897, 978, 1422
LmnI GCTCC 7 cut(s) 184, 365, 512, 1088, 1166, 1358, 1498
Lsp1109I GCAGC 3 cut(s) 188, 985, 1013
LweI GCATC 7 cut(s) 65, 512, 707, 854, 1048, 1061, 1236
MaeI CTAG 4 cut(s) 173, 278, 482, 609
MaeII ACGT 1 cut(s) 1293
MaeIII GTNAC 5 cut(s) 39, 628, 1072, 1133, 1301
MalI GATC 7 cut(s) 21, 111, 476, 728, 899, 980, 1424
MboI GATC 7 cut(s) 19, 109, 474, 726, 897, 978, 1422
MboII GAAGA 8 cut(s) 101, 157, 226, 407, 448, 511, 1496, 1499
MhlI GDGCHC 6 cut(s) 517, 645, 767, 819, 1163, 1394
MlsI TGGCCA 1 cut(s) 354
MluCI AATT 8 cut(s) 153, 193, 260, 411, 534, 562, 578, 674
MluNI TGGCCA 1 cut(s) 354
MmeI TCCRAC 2 cut(s) 1255, 1400
MnlI CCTC 9 cut(s) 59, 173, 239, 283, 323, 1018, 1148, 1150, 1512
Mox20I TGGCCA 1 cut(s) 354
MroI TCCGGA 2 cut(s) 47, 1431
MscI TGGCCA 1 cut(s) 354
MseI TTAA 6 cut(s) 156, 746, 1088, 1100, 1221, 1290
MslI CAYNNNNRTG 2 cut(s) 734, 758
Msp20I TGGCCA 1 cut(s) 354
MspI CCGG 3 cut(s) 48, 182, 1432
MspR9I CCNGG 2 cut(s) 554, 721
Mva1269I GAATGC 2 cut(s) 922, 1386
MvaI CCWGG 2 cut(s) 554, 721
NdeII GATC 7 cut(s) 19, 109, 474, 726, 897, 978, 1422
NlaIII CATG 7 cut(s) 13, 319, 733, 859, 955, 979, 1287
NlaIV GGNNCC 3 cut(s) 271, 1162, 1500
PagI TCATGA 1 cut(s) 975
PcsI WCGNNNNNNNCGW 1 cut(s) 483
PctI GAATGC 2 cut(s) 922, 1386
PfeI GAWTC 1 cut(s) 1468
PkrI GCNGC 3 cut(s) 178, 1000, 1003
Psp6I CCWGG 2 cut(s) 552, 719
PspFI CCCAGC 4 cut(s) 385, 691, 902, 1034
PspGI CCWGG 2 cut(s) 552, 719
PspN4I GGNNCC 3 cut(s) 271, 1162, 1500
PspPI GGNCC 2 cut(s) 68, 739
PstI CTGCAG 3 cut(s) 220, 511, 1003
RsaI GTAC 5 cut(s) 651, 671, 752, 878, 1210
RsaNI GTAC 5 cut(s) 650, 670, 751, 877, 1209
RseI CAYNNNNRTG 2 cut(s) 734, 758
SaqAI TTAA 6 cut(s) 156, 746, 1088, 1100, 1221, 1290
SatI GCNGC 3 cut(s) 177, 999, 1002
Sau3AI GATC 7 cut(s) 19, 109, 474, 726, 897, 978, 1422
Sau96I GGNCC 2 cut(s) 68, 739
ScaI AGTACT 1 cut(s) 651
ScrFI CCNGG 2 cut(s) 554, 721
SduI GDGCHC 6 cut(s) 517, 645, 767, 819, 1163, 1394
SfaNI GCATC 7 cut(s) 65, 512, 707, 854, 1048, 1061, 1236
SfcI CTRYAG 3 cut(s) 216, 507, 999
SmiMI CAYNNNNRTG 2 cut(s) 734, 758
SmlI CTYRAG 2 cut(s) 866, 911
SmoI CTYRAG 2 cut(s) 866, 911
Sse9I AATT 8 cut(s) 153, 193, 260, 411, 534, 562, 578, 674
SsiI CCGC 4 cut(s) 185, 849, 1129, 1388
SspMI CTAG 4 cut(s) 173, 278, 482, 609
StyD4I CCNGG 2 cut(s) 552, 719
StyI CCWWGG 3 cut(s) 130, 767, 930
TaaI ACNGT 2 cut(s) 634, 1168
TaiI ACGT 1 cut(s) 1296
TasI AATT 8 cut(s) 153, 193, 260, 411, 534, 562, 578, 674
TatI WGTACW 4 cut(s) 649, 669, 750, 1208
TfiI GAWTC 1 cut(s) 1468
Tru1I TTAA 6 cut(s) 156, 746, 1088, 1100, 1221, 1290
Tru9I TTAA 6 cut(s) 156, 746, 1088, 1100, 1221, 1290
TseI GCWGC 3 cut(s) 176, 998, 1001
TspDTI ATGAA 4 cut(s) 45, 436, 964, 1494
TspGWI ACGGA 1 cut(s) 506
VneI GTGCAC 1 cut(s) 641
XagI CCTNNNNNAGG 1 cut(s) 134
XapI RAATTY 2 cut(s) 260, 578
XspI CTAG 4 cut(s) 173, 278, 482, 609
ZrmI AGTACT 1 cut(s) 651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.