Prupe.4G062500_v2.0.a1

PCO_ADO

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0020415
Physical Location & Seq
Reverse (-)
2996641 .. 3000080
3440 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G062500.2_v2.0.a1.CDS.5

Sequence Viewer

Length: 723 bp
ATGCCTGTGGTTCAGAAGTTGTATAATGCTTGCAAGGTGTCCTTCTCTAGTAATGGTCCAATATCAGAAGAAGACCTAGAGAGAGTTCGTGCTATCTTAGATGAATTGAAGGCTTCTAATGTGGGTCTCGAACAGGAAGCACAATTGGCACGCGGATTTCATGGTAGCAATGGCAGAAAAGGGCGTAATGGCACTCATCAGTACCCACCCGCAATCAAATATCTGCATTTGCATGAATGCGATAGATTTTCGATAGGAATCTTTTGCATGCCACCTGGCTCTATCATACCCCTTCATAATCATCCTGGAATGACTGTTTTGAGCAAGCTTCTTTATGGTTCTTTGCATGTGAGATCATACGATTGGCTTGATTTACCGGAGTGCTCTGATGTTTCTAAAGCAAGACCTGCAAAGCTTGTTCGCGATTGTGAAATGAGTGCACCTTGTGGTACAACAGTTCTTTATCCAAAAAGTGGAGGCAATATTCATTGTTTCAAAGCCTTGACGTCCTGTGCTCTCTTTGACATTCTTTCACCTCCTTACTCCTCAGAAGATGGGCGACACTGTTCTTATTTCCGGAAGTCCCCTCGTGTAGATCTTCCAAGTCTTGAAGAGTTGTGTGGCGCAGAACCATCTGAAGTAGCTTGGTTGGAAGAGATTCAACCTCCTGAGAACTTCGTAGTCCGCAGGGGGGTGTACAAGGGCCCGACCATTAGAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00430 ko01100 map00430 map01100
Pfam Domains
Protein Families
PCO_ADO

Protein Analysis

241

Amino Acids

26.69

Weight (kDa)

7.58

Isoelectric Point (pI)

56.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 509
Acc36I ACCTGC 1 cut(s) 415
AccB7I CCANNNNNTGG 1 cut(s) 473
AccII CGCG 2 cut(s) 153, 423
AccIII TCCGGA 1 cut(s) 576
AciI CCGC 3 cut(s) 153, 210, 685
AcuI CTGAAG 1 cut(s) 657
AcyI GRCGYC 1 cut(s) 506
AdeI CACNNNGTG 1 cut(s) 446
AfaI GTAC 3 cut(s) 203, 451, 698
AfiI CCNNNNNNNGG 2 cut(s) 473, 691
AgsI TTSAA 4 cut(s) 109, 496, 611, 662
AjnI CCWGG 2 cut(s) 274, 304
AluBI AGCT 3 cut(s) 328, 415, 644
AluI AGCT 3 cut(s) 328, 415, 644
Alw21I GWGCWC 3 cut(s) 386, 442, 517
Alw26I GTCTC 1 cut(s) 131
Alw44I GTGCAC 1 cut(s) 438
Aor13HI TCCGGA 1 cut(s) 576
AoxI GGCC 1 cut(s) 703
ApaI GGGCCC 1 cut(s) 707
ApaLI GTGCAC 1 cut(s) 438
Asp700I GAANNNNTTC 1 cut(s) 657
AspLEI GCGC 1 cut(s) 626
AspS9I GGNCC 3 cut(s) 56, 703, 704
AsuHPI GGTGA 1 cut(s) 525
AvaII GGWCC 1 cut(s) 56
BaeGI GKGCMC 2 cut(s) 442, 707
BaeI ACNNNNGTAYC 2 cut(s) 441, 474
BanII GRGCYC 1 cut(s) 707
BauI CACGAG 1 cut(s) 588
BbsI GAAGAC 1 cut(s) 78
Bbv12I GWGCWC 3 cut(s) 386, 442, 517
BccI CCATC 2 cut(s) 548, 640
BciT130I CCWGG 2 cut(s) 276, 306
BcoDI GTCTC 1 cut(s) 131
BfaI CTAG 2 cut(s) 48, 77
BfuAI ACCTGC 1 cut(s) 415
BglII AGATCT 1 cut(s) 595
Bme1390I CCNGG 2 cut(s) 276, 306
Bme18I GGWCC 1 cut(s) 56
BmgT120I GGNCC 3 cut(s) 56, 703, 704
BmiI GGNNCC 1 cut(s) 705
BmrFI CCNGG 2 cut(s) 276, 306
BpiI GAAGAC 1 cut(s) 78
BsaBI GATNNNNATC 1 cut(s) 257
BsaHI GRCGYC 1 cut(s) 506
BsaI GGTCTC 1 cut(s) 131
BsaWI WCCGGW 2 cut(s) 376, 576
Bsc4I CCNNNNNNNGG 2 cut(s) 473, 691
Bse3DI GCAATG 1 cut(s) 175
Bse8I GATNNNNATC 1 cut(s) 257
BseAI TCCGGA 1 cut(s) 576
BseBI CCWGG 2 cut(s) 276, 306
BseGI GGATG 1 cut(s) 301
BseJI GATNNNNATC 1 cut(s) 257
BseLI CCNNNNNNNGG 2 cut(s) 473, 691
BseMI GCAATG 1 cut(s) 175
BseMII CTCAG 2 cut(s) 561, 660
BseRI GAGGAG 1 cut(s) 535
BseSI GKGCMC 2 cut(s) 442, 707
Bsh1236I CGCG 2 cut(s) 153, 423
BshFI GGCC 1 cut(s) 705
BsiHKAI GWGCWC 3 cut(s) 386, 442, 517
BsiSI CCGG 2 cut(s) 377, 577
BslFI GGGAC 1 cut(s) 568
BslI CCNNNNNNNGG 2 cut(s) 473, 691
BsmAI GTCTC 1 cut(s) 131
BsmFI GGGAC 1 cut(s) 568
BsmI GAATGC 1 cut(s) 242
BsnI GGCC 1 cut(s) 705
Bso31I GGTCTC 1 cut(s) 131
Bsp120I GGGCCC 1 cut(s) 703
Bsp1286I GDGCHC 4 cut(s) 386, 442, 517, 707
Bsp13I TCCGGA 1 cut(s) 576
Bsp1407I TGTACA 1 cut(s) 696
Bsp143I GATC 2 cut(s) 353, 595
Bsp68I TCGCGA 1 cut(s) 423
BspACI CCGC 3 cut(s) 153, 210, 685
BspANI GGCC 1 cut(s) 705
BspCNI CTCAG 2 cut(s) 560, 661
BspEI TCCGGA 1 cut(s) 576
BspFNI CGCG 2 cut(s) 153, 423
BspLI GGNNCC 1 cut(s) 705
BspMI ACCTGC 1 cut(s) 415
BspTNI GGTCTC 1 cut(s) 131
BsrDI GCAATG 1 cut(s) 175
BsrGI TGTACA 1 cut(s) 696
BssMI GATC 2 cut(s) 353, 595
BssNI GRCGYC 1 cut(s) 506
BssSI CACGAG 1 cut(s) 588
Bst2BI CACGAG 1 cut(s) 588
Bst2UI CCWGG 2 cut(s) 276, 306
Bst4CI ACNGT 3 cut(s) 316, 457, 566
Bst6I CTCTTC 2 cut(s) 606, 648
BstACI GRCGYC 1 cut(s) 506
BstAPI GCANNNNNTGC 1 cut(s) 407
BstAUI TGTACA 1 cut(s) 696
BstC8I GCNNGC 4 cut(s) 31, 151, 269, 326
BstDEI CTNAG 3 cut(s) 97, 547, 669
BstF5I GGATG 1 cut(s) 301
BstFNI CGCG 2 cut(s) 153, 423
BstHHI GCGC 1 cut(s) 626
BstKTI GATC 2 cut(s) 356, 598
BstMAI GTCTC 1 cut(s) 131
BstMBI GATC 2 cut(s) 353, 595
BstMWI GCNNNNNNNGC 2 cut(s) 146, 407
BstNI CCWGG 2 cut(s) 276, 306
BstNSI RCATGY 2 cut(s) 271, 350
BstSCI CCNGG 2 cut(s) 274, 304
BstSLI GKGCMC 2 cut(s) 442, 707
BstUI CGCG 2 cut(s) 153, 423
BstV2I GAAGAC 1 cut(s) 78
BstX2I RGATCY 1 cut(s) 595
BstYI RGATCY 1 cut(s) 595
BsuRI GGCC 1 cut(s) 705
BtsCI GGATG 1 cut(s) 301
BtsIMutI CAGTG 1 cut(s) 562
BtuMI TCGCGA 1 cut(s) 423
BveI ACCTGC 1 cut(s) 415
Cac8I GCNNGC 4 cut(s) 31, 151, 269, 326
CfoI GCGC 1 cut(s) 626
Cfr13I GGNCC 3 cut(s) 56, 703, 704
Csp6I GTAC 3 cut(s) 202, 450, 697
CviAII CATG 4 cut(s) 161, 233, 268, 347
CviJI RGCY 8 cut(s) 113, 279, 328, 367, 415, 500, 644, 705
CviKI_1 RGCY 8 cut(s) 113, 279, 328, 367, 415, 500, 644, 705
CviQI GTAC 3 cut(s) 202, 450, 697
DdeI CTNAG 3 cut(s) 97, 547, 669
DpnI GATC 2 cut(s) 355, 597
DpnII GATC 2 cut(s) 353, 595
DraIII CACNNNGTG 1 cut(s) 446
Eam1104I CTCTTC 2 cut(s) 606, 648
EarI CTCTTC 2 cut(s) 606, 648
Eco24I GRGCYC 1 cut(s) 707
Eco31I GGTCTC 1 cut(s) 131
Eco47I GGWCC 1 cut(s) 56
Eco57I CTGAAG 1 cut(s) 657
EcoO109I RGGNCCY 1 cut(s) 703
EcoRII CCWGG 2 cut(s) 274, 304
EcoT38I GRGCYC 1 cut(s) 707
FaeI CATG 4 cut(s) 164, 236, 271, 350
FaiI YATR 9 cut(s) 24, 162, 234, 269, 287, 297, 336, 348, 358
FalI AAGNNNNNCTT 2 cut(s) 26, 58
FaqI GGGAC 1 cut(s) 568
FatI CATG 4 cut(s) 160, 232, 267, 346
FauI CCCGC 1 cut(s) 217
FokI GGATG 1 cut(s) 288
FriOI GRGCYC 1 cut(s) 707
FspBI CTAG 2 cut(s) 48, 77
GlaI GCGC 1 cut(s) 625
HaeIII GGCC 1 cut(s) 705
HapII CCGG 2 cut(s) 377, 577
HhaI GCGC 1 cut(s) 626
Hin1I GRCGYC 1 cut(s) 506
Hin1II CATG 4 cut(s) 164, 236, 271, 350
Hin6I GCGC 1 cut(s) 624
HinP1I GCGC 1 cut(s) 624
HindIII AAGCTT 2 cut(s) 326, 413
HinfI GANTC 2 cut(s) 258, 658
HpaII CCGG 2 cut(s) 377, 577
HphI GGTGA 1 cut(s) 525
Hpy166II GTNNAC 2 cut(s) 440, 697
Hpy188I TCNGA 5 cut(s) 15, 67, 388, 550, 637
Hpy188III TCNNGA 5 cut(s) 128, 422, 577, 608, 668
Hpy8I GTNNAC 2 cut(s) 440, 697
HpyAV CCTTC 3 cut(s) 52, 103, 302
HpyCH4III ACNGT 3 cut(s) 316, 457, 566
HpyCH4IV ACGT 1 cut(s) 506
HpyCH4V TGCA 7 cut(s) 33, 226, 232, 267, 346, 410, 440
HpyF10VI GCNNNNNNNGC 2 cut(s) 146, 407
HpyF3I CTNAG 3 cut(s) 97, 547, 669
HpySE526I ACGT 1 cut(s) 506
Hsp92I GRCGYC 1 cut(s) 506
Hsp92II CATG 4 cut(s) 164, 236, 271, 350
HspAI GCGC 1 cut(s) 624
Kpn2I TCCGGA 1 cut(s) 576
Kzo9I GATC 2 cut(s) 353, 595
MaeI CTAG 2 cut(s) 48, 77
MaeII ACGT 1 cut(s) 506
MalI GATC 2 cut(s) 355, 597
MboI GATC 2 cut(s) 353, 595
MboII GAAGA 6 cut(s) 80, 83, 563, 590, 623, 665
MfeI CAATTG 1 cut(s) 143
MflI RGATCY 1 cut(s) 595
MhlI GDGCHC 4 cut(s) 386, 442, 517, 707
MluCI AATT 2 cut(s) 104, 143
MmeI TCCRAC 1 cut(s) 630
MnlI CCTC 5 cut(s) 470, 546, 556, 597, 675
MroI TCCGGA 1 cut(s) 576
MroXI GAANNNNTTC 1 cut(s) 657
MslI CAYNNNNRTG 1 cut(s) 231
MspI CCGG 2 cut(s) 377, 577
MspR9I CCNGG 2 cut(s) 276, 306
MunI CAATTG 1 cut(s) 143
Mva1269I GAATGC 1 cut(s) 242
MvaI CCWGG 2 cut(s) 276, 306
MvnI CGCG 2 cut(s) 153, 423
MwoI GCNNNNNNNGC 2 cut(s) 146, 407
NdeII GATC 2 cut(s) 353, 595
NlaIII CATG 4 cut(s) 164, 236, 271, 350
NlaIV GGNNCC 1 cut(s) 705
NruI TCGCGA 1 cut(s) 423
NspI RCATGY 2 cut(s) 271, 350
PaeI GCATGC 1 cut(s) 271
PctI GAATGC 1 cut(s) 242
PdmI GAANNNNTTC 1 cut(s) 657
PfeI GAWTC 2 cut(s) 258, 658
PflMI CCANNNNNTGG 1 cut(s) 473
PfoI TCCNGGA 1 cut(s) 304
Psp6I CCWGG 2 cut(s) 274, 304
PspGI CCWGG 2 cut(s) 274, 304
PspN4I GGNNCC 1 cut(s) 705
PspOMI GGGCCC 1 cut(s) 703
PspPI GGNCC 3 cut(s) 56, 703, 704
PsuI RGATCY 1 cut(s) 595
RruI TCGCGA 1 cut(s) 423
RsaI GTAC 3 cut(s) 203, 451, 698
RsaNI GTAC 3 cut(s) 202, 450, 697
RseI CAYNNNNRTG 1 cut(s) 231
Sau3AI GATC 2 cut(s) 353, 595
Sau96I GGNCC 3 cut(s) 56, 703, 704
ScrFI CCNGG 2 cut(s) 276, 306
SduI GDGCHC 4 cut(s) 386, 442, 517, 707
SinI GGWCC 1 cut(s) 56
SmiMI CAYNNNNRTG 1 cut(s) 231
SphI GCATGC 1 cut(s) 271
Sse9I AATT 2 cut(s) 104, 143
SsiI CCGC 3 cut(s) 153, 210, 685
SspI AATATT 1 cut(s) 484
SspMI CTAG 2 cut(s) 48, 77
StyD4I CCNGG 2 cut(s) 274, 304
TaaI ACNGT 3 cut(s) 316, 457, 566
TaiI ACGT 1 cut(s) 509
TaqI TCGA 2 cut(s) 129, 251
TasI AATT 2 cut(s) 104, 143
TatI WGTACW 1 cut(s) 696
TfiI GAWTC 2 cut(s) 258, 658
TscAI CASTG 1 cut(s) 569
TspDTI ATGAA 5 cut(s) 117, 149, 249, 284, 476
TspRI CASTG 1 cut(s) 569
Van91I CCANNNNNTGG 1 cut(s) 473
VneI GTGCAC 1 cut(s) 438
VpaK11BI GGWCC 1 cut(s) 56
XceI RCATGY 2 cut(s) 271, 350
XmnI GAANNNNTTC 1 cut(s) 657
XspI CTAG 2 cut(s) 48, 77
ZraI GACGTC 1 cut(s) 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.